Rorug02G0463200

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004903
Physical Location & Seq
Forward (+)
59435532 .. 59437132
1601 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0463200.1

Sequence Viewer

Length: 666 bp
ATGGCATCAGTCATGGTTTCCCAGGCTCTCTTCTCCCAAACCCCAATCCTAATCAGACCCCCCACCCGCACCCCCAAATTCAAATCCTTCTCCTCTCCCTCTCCCTCCCTCGTCCACATTCGCACTCCCAATCCCAACCTCCGTCTCTCTCTCCGCCGACCCCTCTGCATCTCCGCCACCACCCAAACCCTAACCCCACCGCCGCCGCCTTCTCCGCTCTCCCACGCCCCCACTCGCACTCTCACCACCCTCTTCGCCGTAACCCTAGCCGCCTTCCGCGCCGTCGCCCTCCCCGTCTTCAAATTCGCCTCCCAACACGCCCCCGCCGTCGCCTCCACCGCCGGACCTCTCTTCTTCGCCGCCCTCGGTGACCGCCCCAGCGGCTACCTCAACACTCCGCTCACGGTCGTCGCCGCCGGGCTCTCCAAGTGGCTGGACATCTACAGCGGCGTTCTCATGGTTCGGGTTCTGCTGAGCTGGTTCCCCAATATTCCCTGGGACCGGCAGCCGCTCTCCGCCATCCGGGACCTCTGTGATCCCTACCTCAACCTCTTCCGCAACATCATTCCTCCGATTTTCGACACCTTGGACGTGAGCCCCCTCCTGGCTTTCGCAGTGTTGGGCACGCTCGGCTCGATCCTCAACAACAGTAGGGGAATGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

221

Amino Acids

24.0

Weight (kDa)

10.87

Isoelectric Point (pI)

51.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCB3_YggT PF02325 145 - 213 1.3e-17 CCB3/YggT
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 3 cut(s) 217, 400, 511
AccII CGCG 1 cut(s) 279
AclWI GGATC 2 cut(s) 530, 631
AcsI RAATTY 2 cut(s) 77, 302
AfaI GTAC 1 cut(s) 662
AfiI CCNNNNNNNGG 3 cut(s) 501, 522, 604
AgsI TTSAA 2 cut(s) 82, 301
AjiI CACGTC 1 cut(s) 592
AjnI CCWGG 3 cut(s) 21, 494, 603
AluBI AGCT 1 cut(s) 477
AluI AGCT 1 cut(s) 477
Alw26I GTCTC 1 cut(s) 149
AlwI GGATC 2 cut(s) 530, 631
ApeKI GCWGC 1 cut(s) 505
ApoI RAATTY 2 cut(s) 77, 302
AspLEI GCGC 1 cut(s) 281
AspS9I GGNCC 3 cut(s) 344, 499, 526
AsuC2I CCSGG 2 cut(s) 418, 524
AsuHPI GGTGA 2 cut(s) 235, 380
AvaII GGWCC 3 cut(s) 344, 499, 526
BaeGI GKGCMC 1 cut(s) 626
BanII GRGCYC 2 cut(s) 423, 599
BbsI GAAGAC 1 cut(s) 289
BbvI GCAGC 1 cut(s) 517
BccI CCATC 1 cut(s) 527
BceAI ACGGC 3 cut(s) 242, 266, 311
BcgI CGANNNNNNTGC 2 cut(s) 147, 181
BciT130I CCWGG 3 cut(s) 23, 496, 605
BcnI CCSGG 2 cut(s) 418, 524
BcoDI GTCTC 1 cut(s) 149
BfaI CTAG 1 cut(s) 266
BfmI CTRYAG 1 cut(s) 442
BglI GCCNNNNNGGC 1 cut(s) 381
BisI GCNGC 9 cut(s) 203, 206, 270, 360, 382, 414, 448, 506, 509
BlpI GCTNAGC 1 cut(s) 473
BlsI GCNGC 9 cut(s) 204, 207, 271, 361, 383, 415, 449, 507, 510
Bme1390I CCNGG 5 cut(s) 23, 418, 496, 524, 605
Bme18I GGWCC 3 cut(s) 344, 499, 526
BmgBI CACGTC 1 cut(s) 592
BmgT120I GGNCC 3 cut(s) 344, 499, 526
BmiI GGNNCC 3 cut(s) 482, 500, 527
BmrFI CCNGG 5 cut(s) 23, 418, 496, 524, 605
BmsI GCATC 2 cut(s) 14, 177
BpiI GAAGAC 1 cut(s) 289
Bpu1102I GCTNAGC 1 cut(s) 473
BpuMI CCSGG 2 cut(s) 418, 524
BsaJI CCNNGG 5 cut(s) 21, 364, 494, 495, 585
Bsc4I CCNNNNNNNGG 3 cut(s) 501, 522, 604
Bse118I RCCGGY 1 cut(s) 501
BseBI CCWGG 3 cut(s) 23, 496, 605
BseDI CCNNGG 5 cut(s) 21, 364, 494, 495, 585
BseGI GGATG 1 cut(s) 519
BseLI CCNNNNNNNGG 3 cut(s) 501, 522, 604
BseMII CTCAG 1 cut(s) 464
BseRI GAGGAG 1 cut(s) 82
BseSI GKGCMC 1 cut(s) 626
BseXI GCAGC 1 cut(s) 517
BseYI CCCAGC 1 cut(s) 377
Bsh1236I CGCG 1 cut(s) 279
Bsh1285I CGRYCG 1 cut(s) 408
BsiEI CGRYCG 1 cut(s) 408
BsiSI CCGG 4 cut(s) 342, 417, 502, 523
BslFI GGGAC 2 cut(s) 512, 539
BslI CCNNNNNNNGG 3 cut(s) 501, 522, 604
BsmAI GTCTC 1 cut(s) 149
BsmBI CGTCTC 1 cut(s) 149
BsmFI GGGAC 2 cut(s) 512, 539
Bsp1286I GDGCHC 3 cut(s) 423, 599, 626
Bsp143I GATC 2 cut(s) 535, 636
Bsp1720I GCTNAGC 1 cut(s) 473
BspCNI CTCAG 1 cut(s) 465
BspFNI CGCG 1 cut(s) 279
BspLI GGNNCC 3 cut(s) 482, 500, 527
BspPI GGATC 2 cut(s) 530, 631
BsrBI CCGCTC 3 cut(s) 217, 400, 511
BsrFI RCCGGY 1 cut(s) 501
BssAI RCCGGY 1 cut(s) 501
BssECI CCNNGG 5 cut(s) 21, 364, 494, 495, 585
BssMI GATC 2 cut(s) 535, 636
BssT1I CCWWGG 1 cut(s) 585
Bst2UI CCWGG 3 cut(s) 23, 496, 605
Bst4CI ACNGT 2 cut(s) 406, 650
Bst6I CTCTTC 4 cut(s) 35, 257, 356, 557
BstC8I GCNNGC 1 cut(s) 626
BstDEI CTNAG 1 cut(s) 473
BstEII GGTNACC 1 cut(s) 368
BstF5I GGATG 1 cut(s) 519
BstFNI CGCG 1 cut(s) 279
BstHHI GCGC 1 cut(s) 281
BstKTI GATC 2 cut(s) 538, 639
BstMAI GTCTC 1 cut(s) 149
BstMBI GATC 2 cut(s) 535, 636
BstMCI CGRYCG 1 cut(s) 408
BstMWI GCNNNNNNNGC 5 cut(s) 214, 278, 338, 381, 630
BstNI CCWGG 3 cut(s) 23, 496, 605
BstPI GGTNACC 1 cut(s) 368
BstSCI CCNGG 5 cut(s) 21, 416, 494, 522, 603
BstSFI CTRYAG 1 cut(s) 442
BstSLI GKGCMC 1 cut(s) 626
BstUI CGCG 1 cut(s) 279
BstV1I GCAGC 1 cut(s) 517
BstV2I GAAGAC 1 cut(s) 289
BstXI CCANNNNNNTGG 1 cut(s) 433
BtrI CACGTC 1 cut(s) 592
BtsCI GGATG 1 cut(s) 519
BtsI GCAGTG 1 cut(s) 621
BtsIMutI CAGTG 1 cut(s) 621
Cac8I GCNNGC 1 cut(s) 626
CfoI GCGC 1 cut(s) 281
Cfr10I RCCGGY 1 cut(s) 501
Cfr13I GGNCC 3 cut(s) 344, 499, 526
Csp6I GTAC 1 cut(s) 661
CviAII CATG 2 cut(s) 13, 457
CviQI GTAC 1 cut(s) 661
DdeI CTNAG 1 cut(s) 473
DpnI GATC 2 cut(s) 537, 638
DpnII GATC 2 cut(s) 535, 636
Eam1104I CTCTTC 4 cut(s) 35, 257, 356, 557
EarI CTCTTC 4 cut(s) 35, 257, 356, 557
EciI GGCGGA 3 cut(s) 143, 163, 505
Eco130I CCWWGG 1 cut(s) 585
Eco24I GRGCYC 2 cut(s) 423, 599
Eco47I GGWCC 3 cut(s) 344, 499, 526
Eco91I GGTNACC 1 cut(s) 368
EcoO109I RGGNCCY 1 cut(s) 526
EcoO65I GGTNACC 1 cut(s) 368
EcoRII CCWGG 3 cut(s) 21, 494, 603
EcoT14I CCWWGG 1 cut(s) 585
EcoT38I GRGCYC 2 cut(s) 423, 599
ErhI CCWWGG 1 cut(s) 585
Esp3I CGTCTC 1 cut(s) 149
FaeI CATG 2 cut(s) 16, 460
FaiI YATR 2 cut(s) 14, 458
FaqI GGGAC 2 cut(s) 512, 539
FatI CATG 2 cut(s) 12, 456
FauI CCCGC 2 cut(s) 74, 331
Fnu4HI GCNGC 9 cut(s) 203, 206, 270, 360, 382, 414, 448, 506, 509
FokI GGATG 1 cut(s) 506
FriOI GRGCYC 2 cut(s) 423, 599
Fsp4HI GCNGC 9 cut(s) 203, 206, 270, 360, 382, 414, 448, 506, 509
FspBI CTAG 1 cut(s) 266
GlaI GCGC 1 cut(s) 280
GluI GCNGC 9 cut(s) 203, 206, 270, 360, 382, 414, 448, 506, 509
GsaI CCCAGC 1 cut(s) 381
HapII CCGG 4 cut(s) 342, 417, 502, 523
HhaI GCGC 1 cut(s) 281
Hin1II CATG 2 cut(s) 16, 460
Hin6I GCGC 1 cut(s) 279
HinP1I GCGC 1 cut(s) 279
HpaII CCGG 4 cut(s) 342, 417, 502, 523
HphI GGTGA 2 cut(s) 235, 380
Hpy166II GTNNAC 1 cut(s) 115
Hpy188I TCNGA 2 cut(s) 56, 573
Hpy8I GTNNAC 1 cut(s) 115
Hpy99I CGWCG 3 cut(s) 287, 332, 413
HpyAV CCTTC 3 cut(s) 97, 219, 283
HpyCH4III ACNGT 2 cut(s) 406, 650
HpyCH4IV ACGT 1 cut(s) 591
HpyCH4V TGCA 1 cut(s) 168
HpyF10VI GCNNNNNNNGC 5 cut(s) 214, 278, 338, 381, 630
HpyF3I CTNAG 1 cut(s) 473
HpySE526I ACGT 1 cut(s) 591
Hsp92II CATG 2 cut(s) 16, 460
HspAI GCGC 1 cut(s) 279
Kzo9I GATC 2 cut(s) 535, 636
Lsp1109I GCAGC 1 cut(s) 517
LweI GCATC 2 cut(s) 14, 177
MaeI CTAG 1 cut(s) 266
MaeII ACGT 1 cut(s) 591
MaeIII GTNAC 2 cut(s) 259, 368
MalI GATC 2 cut(s) 537, 638
MbiI CCGCTC 3 cut(s) 217, 400, 511
MboI GATC 2 cut(s) 535, 636
MboII GAAGA 6 cut(s) 22, 244, 289, 343, 346, 544
MhlI GDGCHC 3 cut(s) 423, 599, 626
MluCI AATT 2 cut(s) 77, 302
MspA1I CMGCKG 2 cut(s) 381, 447
MspI CCGG 4 cut(s) 342, 417, 502, 523
MspR9I CCNGG 5 cut(s) 23, 418, 496, 524, 605
MvaI CCWGG 3 cut(s) 23, 496, 605
MvnI CGCG 1 cut(s) 279
MwoI GCNNNNNNNGC 5 cut(s) 214, 278, 338, 381, 630
NciI CCSGG 2 cut(s) 418, 524
NdeII GATC 2 cut(s) 535, 636
NlaIII CATG 2 cut(s) 16, 460
NlaIV GGNNCC 3 cut(s) 482, 500, 527
NmeAIII GCCGAG 1 cut(s) 609
NmuCI GTSAC 1 cut(s) 368
PasI CCCWGGG 1 cut(s) 495
PcsI WCGNNNNNNNCGW 3 cut(s) 291, 324, 632
PfoI TCCNGGA 1 cut(s) 522
PkrI GCNGC 9 cut(s) 204, 207, 271, 361, 383, 415, 449, 507, 510
PpuMI RGGWCCY 1 cut(s) 526
Psp5II RGGWCCY 1 cut(s) 526
Psp6I CCWGG 3 cut(s) 21, 494, 603
PspEI GGTNACC 1 cut(s) 368
PspFI CCCAGC 1 cut(s) 377
PspGI CCWGG 3 cut(s) 21, 494, 603
PspN4I GGNNCC 3 cut(s) 482, 500, 527
PspPI GGNCC 3 cut(s) 344, 499, 526
PspPPI RGGWCCY 1 cut(s) 526
RsaI GTAC 1 cut(s) 662
RsaNI GTAC 1 cut(s) 661
SatI GCNGC 9 cut(s) 203, 206, 270, 360, 382, 414, 448, 506, 509
Sau3AI GATC 2 cut(s) 535, 636
Sau96I GGNCC 3 cut(s) 344, 499, 526
ScrFI CCNGG 5 cut(s) 23, 418, 496, 524, 605
SduI GDGCHC 3 cut(s) 423, 599, 626
SetI ASST 9 cut(s) 141, 349, 390, 479, 531, 546, 552, 587, 594
SfaNI GCATC 2 cut(s) 14, 177
SfcI CTRYAG 1 cut(s) 442
SinI GGWCC 3 cut(s) 344, 499, 526
Sse9I AATT 2 cut(s) 77, 302
SspI AATATT 1 cut(s) 490
SspMI CTAG 1 cut(s) 266
StyD4I CCNGG 5 cut(s) 21, 416, 494, 522, 603
StyI CCWWGG 1 cut(s) 585
TaaI ACNGT 2 cut(s) 406, 650
TaiI ACGT 1 cut(s) 594
TaqI TCGA 2 cut(s) 579, 635
TasI AATT 2 cut(s) 77, 302
TatI WGTACW 1 cut(s) 660
TauI GCSGC 8 cut(s) 205, 208, 272, 362, 384, 416, 450, 511
TscAI CASTG 1 cut(s) 621
TseFI GTSAC 1 cut(s) 368
TseI GCWGC 1 cut(s) 505
Tsp45I GTSAC 1 cut(s) 368
TspGWI ACGGA 1 cut(s) 131
TspRI CASTG 1 cut(s) 621
VpaK11BI GGWCC 3 cut(s) 344, 499, 526
XapI RAATTY 2 cut(s) 77, 302
XspI CTAG 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.