FvH4_7g09550

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0012492
Physical Location & Seq
Reverse (-)
9175646 .. 9176470
825 bp
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UTR
Exon/CDS
Intron
FvH4_7g09550.t1

Sequence Viewer

Length: 825 bp
ATGGCTAAGAAAATCAACATAGAGAGTGATCCTCATGGAAACCTCCACACCTCCTCTTCTTCCTCCTCCTCCACCTCAATAGTAACAACAACAACATCCCATGATTCTGTGATTGATAAAAGAACAGAGAGTGATCAGAATTCAGAGAAGCTCTCAAGCTCAACAGGGCAAGAGGATAACAATGGGAGTAACAATGAGCCTAACACCAAGAAGAGGCAGAGGAGCAGCAATGATCATGGCGATGGAGGCAAGCACCCGACTTTTCGAGGAGTACGAATGCGTTCTTGGGGCAAATGGGTGTCTGAAATCCGTGAGCCGAGGAAGAAATCAAGAATTTGGTTAGGGACATATCCTACAGCTGAAATGGCTGCCCGAGCGCACGATGTGGCTGCTCGGGCTATCAAAGGTGCTTCTGCTTATCTCAATTTCCCTGAATTGGCTCAAACTCTTCCACAGCCGGCCTCTGCTTCTCCCAAGGACATTCAAGCTGCTGCTGCAAAAGCTGCTTCTGCTTTGGAGGAGATGAAGAAATGTGGAGCTGAAGTGGAAGAAGAAGAAGAGCAAGCAACACAACCCAGCAAAGATGAAGAGACAACTACTACTACTGCTAATACTAGTACTAGTACTAGTAACAGTACAGCTTGTTCTTCATCTTCCTCTTCAGTTGATTATGATGACAAATTGTTTGACCTTCCAGATCTTTTCATGGATGGCACAAATGGAAGTTATCAGTTTTGCTTTTCTTCTTCTTCATCATCATCATGGCATCTCTGTGCAGCAGATACTGGATTCAGACTAGAAGAGCCATTCTCGTGGGAGTATTAA

Protein Analysis

275

Amino Acids

29.76

Weight (kDa)

5.24

Isoelectric Point (pI)

53.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 87 - 136 3.7e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012492)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44940
fragaria_vesca FvH4_7g09550
malus_domestica MD02G1217900.v1.1 MD07G1099400.v1.1
prunus_persica Prupe.2G123200_v2.0.a1
pyrus_communis pycom07g08150 pycom1310g00030
rosa_chinensis RchiOBHm_Chr1g0346421
rosa_laevigata RLG00000007751 RLG00000028801
rosa_multiflora Rmu_sc0003363.1_g000069 Rmu_sc0022454.1_g000001
rosa_roxburghii Rroxscaffold_4G00308600
rosa_rugosa Rorug01G0179600
rosa_samantha Rh1AG199100 Rh1BG164800 Rh1CG184100 Rh1DG195600
rosa_wichuraiana Rw1G016700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 23
AcsI RAATTY 2 cut(s) 139, 333
AcuI CTGAAG 2 cut(s) 561, 645
AdeI CACNNNGTG 1 cut(s) 385
AfaI GTAC 4 cut(s) 273, 619, 625, 637
AfiI CCNNNNNNNGG 2 cut(s) 213, 436
AgsI TTSAA 1 cut(s) 485
AhlI ACTAGT 3 cut(s) 614, 620, 626
AjuI GAANNNNNNNTTGG 2 cut(s) 268, 300
AluBI AGCT 7 cut(s) 151, 159, 359, 488, 503, 539, 641
AluI AGCT 7 cut(s) 151, 159, 359, 488, 503, 539, 641
Alw26I GTCTC 1 cut(s) 584
AlwI GGATC 1 cut(s) 23
AlwNI CAGNNNCTG 1 cut(s) 785
Ama87I CYCGRG 2 cut(s) 372, 393
AoxI GGCC 1 cut(s) 459
ApeKI GCWGC 8 cut(s) 225, 368, 389, 488, 491, 494, 503, 776
ApoI RAATTY 2 cut(s) 139, 333
ArsI GACNNNNNNTTYG 2 cut(s) 668, 700
Asp700I GAANNNNTTC 1 cut(s) 280
AspLEI GCGC 1 cut(s) 379
AvaI CYCGRG 2 cut(s) 372, 393
BauI CACGAG 1 cut(s) 811
BbvI GCAGC 8 cut(s) 237, 355, 376, 475, 478, 481, 490, 788
BccI CCATC 2 cut(s) 236, 704
BcgI CGANNNNNNTGC 2 cut(s) 371, 405
BclI TGATCA 2 cut(s) 133, 232
BcoDI GTCTC 1 cut(s) 584
BcuI ACTAGT 3 cut(s) 614, 620, 626
BfaI CTAG 4 cut(s) 615, 621, 627, 797
BfmI CTRYAG 1 cut(s) 354
BglII AGATCT 1 cut(s) 697
BisI GCNGC 8 cut(s) 226, 369, 390, 489, 492, 495, 504, 777
BlsI GCNGC 8 cut(s) 227, 370, 391, 490, 493, 496, 505, 778
BmcAI AGTACT 2 cut(s) 619, 625
BmeT110I CYCGRG 2 cut(s) 372, 393
BmsI GCATC 1 cut(s) 775
BplI GAGNNNNNCTC 5 cut(s) 16, 48, 137, 169, 794
BpuEI CTTGAG 1 cut(s) 139
BsaJI CCNNGG 2 cut(s) 317, 474
BsaXI ACNNNNNCTCC 2 cut(s) 528, 558
Bsc4I CCNNNNNNNGG 2 cut(s) 213, 436
Bse118I RCCGGY 1 cut(s) 457
Bse1I ACTGG 1 cut(s) 790
Bse3DI GCAATG 1 cut(s) 235
BseDI CCNNGG 2 cut(s) 317, 474
BseGI GGATG 2 cut(s) 95, 715
BseLI CCNNNNNNNGG 2 cut(s) 213, 436
BseMI GCAATG 1 cut(s) 235
BseNI ACTGG 1 cut(s) 790
BseRI GAGGAG 6 cut(s) 43, 55, 58, 235, 282, 533
BseXI GCAGC 8 cut(s) 237, 355, 376, 475, 478, 481, 490, 788
BseYI CCCAGC 1 cut(s) 575
BsgI GTGCAG 1 cut(s) 795
BshFI GGCC 1 cut(s) 461
BsiHKCI CYCGRG 2 cut(s) 372, 393
BsiSI CCGG 1 cut(s) 458
BslFI GGGAC 1 cut(s) 358
BslI CCNNNNNNNGG 2 cut(s) 213, 436
BsmAI GTCTC 1 cut(s) 584
BsmFI GGGAC 1 cut(s) 358
BsmI GAATGC 1 cut(s) 282
BsnI GGCC 1 cut(s) 461
BsoBI CYCGRG 2 cut(s) 372, 393
Bsp143I GATC 4 cut(s) 28, 133, 232, 697
BspANI GGCC 1 cut(s) 461
BspPI GGATC 1 cut(s) 23
BspQI GCTCTTC 2 cut(s) 552, 795
BsrDI GCAATG 1 cut(s) 235
BsrFI RCCGGY 1 cut(s) 457
BsrI ACTGG 1 cut(s) 790
BssAI RCCGGY 1 cut(s) 457
BssECI CCNNGG 2 cut(s) 317, 474
BssMI GATC 4 cut(s) 28, 133, 232, 697
BssSI CACGAG 1 cut(s) 811
BssT1I CCWWGG 1 cut(s) 474
Bst2BI CACGAG 1 cut(s) 811
Bst4CI ACNGT 1 cut(s) 635
Bst6I CTCTTC 7 cut(s) 61, 206, 453, 552, 582, 664, 795
BstAPI GCANNNNNTGC 1 cut(s) 503
BstC8I GCNNGC 3 cut(s) 251, 459, 564
BstDEI CTNAG 1 cut(s) 6
BstF5I GGATG 2 cut(s) 95, 715
BstHHI GCGC 1 cut(s) 379
BstKTI GATC 4 cut(s) 31, 136, 235, 700
BstMAI GTCTC 1 cut(s) 584
BstMBI GATC 4 cut(s) 28, 133, 232, 697
BstMWI GCNNNNNNNGC 8 cut(s) 246, 365, 374, 395, 494, 500, 503, 509
BstSFI CTRYAG 1 cut(s) 354
BstV1I GCAGC 8 cut(s) 237, 355, 376, 475, 478, 481, 490, 788
BstX2I RGATCY 1 cut(s) 697
BstXI CCANNNNNNTGG 1 cut(s) 813
BstYI RGATCY 1 cut(s) 697
BsuRI GGCC 1 cut(s) 461
BtgZI GCGATG 1 cut(s) 255
BtsCI GGATG 2 cut(s) 95, 715
Cac8I GCNNGC 3 cut(s) 251, 459, 564
CaiI CAGNNNCTG 1 cut(s) 785
CfoI GCGC 1 cut(s) 379
Cfr10I RCCGGY 1 cut(s) 457
Csp6I GTAC 4 cut(s) 272, 618, 624, 636
CviAII CATG 5 cut(s) 35, 101, 236, 706, 762
CviQI GTAC 4 cut(s) 272, 618, 624, 636
DdeI CTNAG 1 cut(s) 6
DpnI GATC 4 cut(s) 30, 135, 234, 699
DpnII GATC 4 cut(s) 28, 133, 232, 697
DraIII CACNNNGTG 1 cut(s) 385
Eam1104I CTCTTC 7 cut(s) 61, 206, 453, 552, 582, 664, 795
EarI CTCTTC 7 cut(s) 61, 206, 453, 552, 582, 664, 795
Eco130I CCWWGG 1 cut(s) 474
Eco57I CTGAAG 2 cut(s) 561, 645
Eco88I CYCGRG 2 cut(s) 372, 393
EcoRI GAATTC 1 cut(s) 139
EcoT14I CCWWGG 1 cut(s) 474
ErhI CCWWGG 1 cut(s) 474
FaeI CATG 5 cut(s) 38, 104, 239, 709, 765
FaiI YATR 8 cut(s) 20, 36, 102, 237, 349, 672, 707, 763
FaqI GGGAC 1 cut(s) 358
FatI CATG 5 cut(s) 34, 100, 235, 705, 761
FbaI TGATCA 2 cut(s) 133, 232
Fnu4HI GCNGC 8 cut(s) 226, 369, 390, 489, 492, 495, 504, 777
FokI GGATG 2 cut(s) 82, 722
Fsp4HI GCNGC 8 cut(s) 226, 369, 390, 489, 492, 495, 504, 777
FspBI CTAG 4 cut(s) 615, 621, 627, 797
GlaI GCGC 1 cut(s) 378
GluI GCNGC 8 cut(s) 226, 369, 390, 489, 492, 495, 504, 777
GsaI CCCAGC 1 cut(s) 579
HaeIII GGCC 1 cut(s) 461
HapII CCGG 1 cut(s) 458
HhaI GCGC 1 cut(s) 379
Hin1II CATG 5 cut(s) 38, 104, 239, 709, 765
Hin6I GCGC 1 cut(s) 377
HinP1I GCGC 1 cut(s) 377
HinfI GANTC 2 cut(s) 104, 789
HpaII CCGG 1 cut(s) 458
Hpy188I TCNGA 4 cut(s) 138, 145, 304, 794
Hpy188III TCNNGA 2 cut(s) 330, 695
HpyAV CCTTC 1 cut(s) 701
HpyCH4III ACNGT 1 cut(s) 635
HpyCH4V TGCA 2 cut(s) 497, 776
HpyF10VI GCNNNNNNNGC 8 cut(s) 246, 365, 374, 395, 494, 500, 503, 509
HpyF3I CTNAG 1 cut(s) 6
Hsp92II CATG 5 cut(s) 38, 104, 239, 709, 765
HspAI GCGC 1 cut(s) 377
KroI GCCGGC 1 cut(s) 457
KroNI GCCGGC 1 cut(s) 459
Ksp22I TGATCA 2 cut(s) 133, 232
Kzo9I GATC 4 cut(s) 28, 133, 232, 697
LguI GCTCTTC 2 cut(s) 552, 795
LmnI GCTCC 2 cut(s) 222, 536
LpnPI CCDG 6 cut(s) 150, 444, 471, 589, 708, 771
Lsp1109I GCAGC 8 cut(s) 237, 355, 376, 475, 478, 481, 490, 788
LweI GCATC 1 cut(s) 775
MaeI CTAG 4 cut(s) 615, 621, 627, 797
MaeIII GTNAC 3 cut(s) 82, 188, 629
MalI GATC 4 cut(s) 30, 135, 234, 699
MboI GATC 4 cut(s) 28, 133, 232, 697
MflI RGATCY 1 cut(s) 697
MluCI AATT 5 cut(s) 139, 333, 424, 434, 680
MroNI GCCGGC 1 cut(s) 457
MroXI GAANNNNTTC 1 cut(s) 280
MseI TTAA 1 cut(s) 823
MslI CAYNNNNRTG 4 cut(s) 240, 760, 771, 811
MspA1I CMGCKG 1 cut(s) 359
MspI CCGG 1 cut(s) 458
Mva1269I GAATGC 1 cut(s) 282
MwoI GCNNNNNNNGC 8 cut(s) 246, 365, 374, 395, 494, 500, 503, 509
NaeI GCCGGC 1 cut(s) 459
NdeII GATC 4 cut(s) 28, 133, 232, 697
NgoMIV GCCGGC 1 cut(s) 457
NlaIII CATG 5 cut(s) 38, 104, 239, 709, 765
NmeAIII GCCGAG 1 cut(s) 342
PciSI GCTCTTC 2 cut(s) 552, 795
PcsI WCGNNNNNNNCGW 1 cut(s) 271
PctI GAATGC 1 cut(s) 282
PdiI GCCGGC 1 cut(s) 459
PdmI GAANNNNTTC 1 cut(s) 280
PfeI GAWTC 2 cut(s) 104, 789
PkrI GCNGC 8 cut(s) 227, 370, 391, 490, 493, 496, 505, 778
PspFI CCCAGC 1 cut(s) 575
PsrI GAACNNNNNNTAC 2 cut(s) 628, 660
PstNI CAGNNNCTG 1 cut(s) 785
PsuI RGATCY 1 cut(s) 697
PvuII CAGCTG 1 cut(s) 359
RsaI GTAC 4 cut(s) 273, 619, 625, 637
RsaNI GTAC 4 cut(s) 272, 618, 624, 636
RseI CAYNNNNRTG 4 cut(s) 240, 760, 771, 811
SapI GCTCTTC 2 cut(s) 552, 795
SaqAI TTAA 1 cut(s) 823
SatI GCNGC 8 cut(s) 226, 369, 390, 489, 492, 495, 504, 777
Sau3AI GATC 4 cut(s) 28, 133, 232, 697
ScaI AGTACT 2 cut(s) 619, 625
SfaNI GCATC 1 cut(s) 775
SfcI CTRYAG 1 cut(s) 354
SmiMI CAYNNNNRTG 4 cut(s) 240, 760, 771, 811
SmlI CTYRAG 1 cut(s) 154
SmoI CTYRAG 1 cut(s) 154
SpeI ACTAGT 3 cut(s) 614, 620, 626
Sse9I AATT 5 cut(s) 139, 333, 424, 434, 680
SspMI CTAG 4 cut(s) 615, 621, 627, 797
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 1 cut(s) 635
TaqI TCGA 1 cut(s) 265
TasI AATT 5 cut(s) 139, 333, 424, 434, 680
TatI WGTACW 3 cut(s) 617, 623, 635
TfiI GAWTC 2 cut(s) 104, 789
Tru1I TTAA 1 cut(s) 823
Tru9I TTAA 1 cut(s) 823
TseI GCWGC 8 cut(s) 225, 368, 389, 488, 491, 494, 503, 776
TspDTI ATGAA 5 cut(s) 539, 600, 639, 694, 741
TspGWI ACGGA 1 cut(s) 299
XapI RAATTY 2 cut(s) 139, 333
XmnI GAANNNNTTC 1 cut(s) 280
XspI CTAG 4 cut(s) 615, 621, 627, 797
ZrmI AGTACT 2 cut(s) 619, 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.