Rw1G016700

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0012492
Physical Location & Seq
Reverse (-)
37217813 .. 37218629
817 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G016700.1

Sequence Viewer

Length: 774 bp
ATGGCTAAGAAAATCAACATAGAGAGTGATGCTCAAGCAAACCTCCACACATCCTCTTCGTCCTCCTCCTCCTCCTCCTCAACCTTGACAACCCATGATTCTTTGATCGATAAAAGCACAGAGAATGATCAGAATTCAGACAAGGTCTCAAGCTCAAAGGGGCAAGAGCTAAACAATGGGAGTAATGAGCCTAACACCAAGAAGAGGCAGAGGAGCAGCAGCACTGATCATGGCGATGGAGGCAGGCACCCGACTTTTCGGGGAGTACGAATGCGCTCTTGGGGCAAATGGGTGTCCGAAATCCGCGAGCCGAGGAAGAAGTCAAGAATTTGGTTAGGGACATACCCAACAGCTGAAATGGCAGCCCGAGCGCACGATGTGGCTGCTCGGGCTATCAAAGGTGCTTCTGCTTACCTAAATTTCCCTGAATTGGCTCAAACGCTTCCACAACCGGCTTCTGCTTCTCCCAAGGACATTCAAGCTGCTGCTGCAAAAGCTGCTTCTGCTTTTGAGGAGGAAGCAACACTGCCCAGCAAAGATGAACAGACAACTACTACTGCTAATACAAGTATCAGTACTAGTAATAGTACAGCTTGTTCTTCATCTTCCACCTCAAATGATTATGATGATAAATTGTTTGACCTTCCAGATCTTTTCATGGATGGCACAAATGGGAGTGATCAATTTTGCTTTTCTTCTTCATCATCATCATGGCATCTGTGTGCAGCAGATACTGGATTCAGACTAGAAGAGCCATTCTTGTGGGAGTATTAA

Protein Analysis

257

Amino Acids

27.84

Weight (kDa)

5.55

Isoelectric Point (pI)

52.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 85 - 134 3.3e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012492)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44940
fragaria_vesca FvH4_7g09550
malus_domestica MD02G1217900.v1.1 MD07G1099400.v1.1
prunus_persica Prupe.2G123200_v2.0.a1
pyrus_communis pycom07g08150 pycom1310g00030
rosa_chinensis RchiOBHm_Chr1g0346421
rosa_laevigata RLG00000007751 RLG00000028801
rosa_multiflora Rmu_sc0003363.1_g000069 Rmu_sc0022454.1_g000001
rosa_roxburghii Rroxscaffold_4G00308600
rosa_rugosa Rorug01G0179600
rosa_samantha Rh1AG199100 Rh1BG164800 Rh1CG184100 Rh1DG195600
rosa_wichuraiana Rw1G016700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 246
AccII CGCG 1 cut(s) 306
AciI CCGC 1 cut(s) 304
AcsI RAATTY 3 cut(s) 133, 327, 418
AdeI CACNNNGTG 1 cut(s) 379
AfaI GTAC 3 cut(s) 267, 577, 589
AfiI CCNNNNNNNGG 2 cut(s) 204, 430
AgsI TTSAA 1 cut(s) 479
AhlI ACTAGT 1 cut(s) 578
AjuI GAANNNNNNNTTGG 2 cut(s) 262, 294
AluBI AGCT 6 cut(s) 153, 169, 353, 482, 497, 593
AluI AGCT 6 cut(s) 153, 169, 353, 482, 497, 593
Alw26I GTCTC 1 cut(s) 151
AlwNI CAGNNNCTG 1 cut(s) 734
Ama87I CYCGRG 2 cut(s) 366, 387
ApeKI GCWGC 9 cut(s) 216, 219, 362, 383, 482, 485, 488, 497, 725
ApoI RAATTY 3 cut(s) 133, 327, 418
AspLEI GCGC 2 cut(s) 276, 373
AvaI CYCGRG 2 cut(s) 366, 387
BanI GGYRCC 1 cut(s) 246
BbvI GCAGC 9 cut(s) 228, 231, 370, 374, 469, 472, 475, 484, 737
BccI CCATC 2 cut(s) 230, 656
BcgI CGANNNNNNTGC 2 cut(s) 365, 399
BclI TGATCA 3 cut(s) 127, 226, 679
BcoDI GTCTC 1 cut(s) 151
BcuI ACTAGT 1 cut(s) 578
BfaI CTAG 2 cut(s) 579, 746
BglII AGATCT 1 cut(s) 649
BisI GCNGC 9 cut(s) 217, 220, 363, 384, 483, 486, 489, 498, 726
BlsI GCNGC 9 cut(s) 218, 221, 364, 385, 484, 487, 490, 499, 727
BmcAI AGTACT 1 cut(s) 577
BmeT110I CYCGRG 2 cut(s) 366, 387
BmiI GGNNCC 1 cut(s) 248
BmsI GCATC 2 cut(s) 19, 724
BplI GAGNNNNNCTC 2 cut(s) 16, 48
BpuEI CTTGAG 2 cut(s) 18, 133
Bsa29I ATCGAT 1 cut(s) 108
BsaI GGTCTC 1 cut(s) 151
BsaJI CCNNGG 2 cut(s) 311, 468
Bsc4I CCNNNNNNNGG 2 cut(s) 204, 430
Bse118I RCCGGY 1 cut(s) 451
Bse1I ACTGG 1 cut(s) 739
BseCI ATCGAT 1 cut(s) 108
BseDI CCNNGG 2 cut(s) 311, 468
BseGI GGATG 2 cut(s) 50, 667
BseLI CCNNNNNNNGG 2 cut(s) 204, 430
BseNI ACTGG 1 cut(s) 739
BseRI GAGGAG 7 cut(s) 55, 58, 61, 64, 67, 226, 527
BseXI GCAGC 9 cut(s) 228, 231, 370, 374, 469, 472, 475, 484, 737
BseYI CCCAGC 1 cut(s) 530
BsgI GTGCAG 1 cut(s) 744
Bsh1236I CGCG 1 cut(s) 306
BshNI GGYRCC 1 cut(s) 246
BshVI ATCGAT 1 cut(s) 108
BsiHKCI CYCGRG 2 cut(s) 366, 387
BsiSI CCGG 1 cut(s) 452
BslFI GGGAC 1 cut(s) 352
BslI CCNNNNNNNGG 2 cut(s) 204, 430
BsmAI GTCTC 1 cut(s) 151
BsmFI GGGAC 1 cut(s) 352
BsmI GAATGC 1 cut(s) 276
Bso31I GGTCTC 1 cut(s) 151
BsoBI CYCGRG 2 cut(s) 366, 387
Bsp143I GATC 5 cut(s) 105, 127, 226, 649, 679
BspACI CCGC 1 cut(s) 304
BspDI ATCGAT 1 cut(s) 108
BspFNI CGCG 1 cut(s) 306
BspLI GGNNCC 1 cut(s) 248
BspQI GCTCTTC 1 cut(s) 744
BspT107I GGYRCC 1 cut(s) 246
BspTNI GGTCTC 1 cut(s) 151
BsrFI RCCGGY 1 cut(s) 451
BsrI ACTGG 1 cut(s) 739
BssAI RCCGGY 1 cut(s) 451
BssECI CCNNGG 2 cut(s) 311, 468
BssMI GATC 5 cut(s) 105, 127, 226, 649, 679
BssT1I CCWWGG 1 cut(s) 468
Bst6I CTCTTC 3 cut(s) 61, 197, 744
BstAPI GCANNNNNTGC 1 cut(s) 497
BstC8I GCNNGC 2 cut(s) 245, 308
BstDEI CTNAG 1 cut(s) 6
BstF5I GGATG 2 cut(s) 50, 667
BstFNI CGCG 1 cut(s) 306
BstHHI GCGC 2 cut(s) 276, 373
BstKTI GATC 5 cut(s) 108, 130, 229, 652, 682
BstMAI GTCTC 1 cut(s) 151
BstMBI GATC 5 cut(s) 105, 127, 226, 649, 679
BstMWI GCNNNNNNNGC 9 cut(s) 240, 282, 359, 368, 389, 488, 494, 497, 503
BstUI CGCG 1 cut(s) 306
BstV1I GCAGC 9 cut(s) 228, 231, 370, 374, 469, 472, 475, 484, 737
BstX2I RGATCY 1 cut(s) 649
BstXI CCANNNNNNTGG 1 cut(s) 762
BstYI RGATCY 1 cut(s) 649
Bsu15I ATCGAT 1 cut(s) 108
BsuTUI ATCGAT 1 cut(s) 108
BtgZI GCGATG 1 cut(s) 249
BtsCI GGATG 2 cut(s) 50, 667
BtsI GCAGTG 1 cut(s) 524
BtsIMutI CAGTG 2 cut(s) 222, 524
Cac8I GCNNGC 2 cut(s) 245, 308
CaiI CAGNNNCTG 1 cut(s) 734
CfoI GCGC 2 cut(s) 276, 373
Cfr10I RCCGGY 1 cut(s) 451
ClaI ATCGAT 1 cut(s) 108
Csp6I GTAC 3 cut(s) 266, 576, 588
CviAII CATG 4 cut(s) 95, 230, 658, 711
CviQI GTAC 3 cut(s) 266, 576, 588
DdeI CTNAG 1 cut(s) 6
DpnI GATC 5 cut(s) 107, 129, 228, 651, 681
DpnII GATC 5 cut(s) 105, 127, 226, 649, 679
DraIII CACNNNGTG 1 cut(s) 379
Eam1104I CTCTTC 3 cut(s) 61, 197, 744
EarI CTCTTC 3 cut(s) 61, 197, 744
Eco130I CCWWGG 1 cut(s) 468
Eco31I GGTCTC 1 cut(s) 151
Eco88I CYCGRG 2 cut(s) 366, 387
EcoRI GAATTC 1 cut(s) 133
EcoT14I CCWWGG 1 cut(s) 468
ErhI CCWWGG 1 cut(s) 468
FaeI CATG 4 cut(s) 98, 233, 661, 714
FaiI YATR 7 cut(s) 20, 96, 231, 343, 624, 659, 712
FaqI GGGAC 1 cut(s) 352
FatI CATG 4 cut(s) 94, 229, 657, 710
FbaI TGATCA 3 cut(s) 127, 226, 679
Fnu4HI GCNGC 9 cut(s) 217, 220, 363, 384, 483, 486, 489, 498, 726
FokI GGATG 2 cut(s) 37, 674
Fsp4HI GCNGC 9 cut(s) 217, 220, 363, 384, 483, 486, 489, 498, 726
FspBI CTAG 2 cut(s) 579, 746
GlaI GCGC 2 cut(s) 275, 372
GluI GCNGC 9 cut(s) 217, 220, 363, 384, 483, 486, 489, 498, 726
GsaI CCCAGC 1 cut(s) 534
HapII CCGG 1 cut(s) 452
HhaI GCGC 2 cut(s) 276, 373
Hin1II CATG 4 cut(s) 98, 233, 661, 714
Hin6I GCGC 2 cut(s) 274, 371
HinP1I GCGC 2 cut(s) 274, 371
HinfI GANTC 2 cut(s) 98, 738
HpaII CCGG 1 cut(s) 452
Hpy188I TCNGA 4 cut(s) 132, 139, 298, 743
Hpy188III TCNNGA 2 cut(s) 324, 647
HpyAV CCTTC 1 cut(s) 653
HpyCH4V TGCA 2 cut(s) 491, 725
HpyF10VI GCNNNNNNNGC 9 cut(s) 240, 282, 359, 368, 389, 488, 494, 497, 503
HpyF3I CTNAG 1 cut(s) 6
Hsp92II CATG 4 cut(s) 98, 233, 661, 714
HspAI GCGC 2 cut(s) 274, 371
Ksp22I TGATCA 3 cut(s) 127, 226, 679
Kzo9I GATC 5 cut(s) 105, 127, 226, 649, 679
LguI GCTCTTC 1 cut(s) 744
LmnI GCTCC 1 cut(s) 213
LpnPI CCDG 6 cut(s) 229, 438, 465, 544, 660, 720
Lsp1109I GCAGC 9 cut(s) 228, 231, 370, 374, 469, 472, 475, 484, 737
LweI GCATC 2 cut(s) 19, 724
MaeI CTAG 2 cut(s) 579, 746
MalI GATC 5 cut(s) 107, 129, 228, 651, 681
MboI GATC 5 cut(s) 105, 127, 226, 649, 679
MboII GAAGA 8 cut(s) 48, 214, 328, 591, 597, 687, 690, 761
MflI RGATCY 1 cut(s) 649
MluCI AATT 6 cut(s) 133, 327, 418, 428, 632, 683
MseI TTAA 1 cut(s) 772
MslI CAYNNNNRTG 4 cut(s) 234, 709, 720, 760
MspA1I CMGCKG 1 cut(s) 353
MspI CCGG 1 cut(s) 452
Mva1269I GAATGC 1 cut(s) 276
MvnI CGCG 1 cut(s) 306
MwoI GCNNNNNNNGC 9 cut(s) 240, 282, 359, 368, 389, 488, 494, 497, 503
NdeII GATC 5 cut(s) 105, 127, 226, 649, 679
NlaIII CATG 4 cut(s) 98, 233, 661, 714
NlaIV GGNNCC 1 cut(s) 248
NmeAIII GCCGAG 1 cut(s) 336
PciSI GCTCTTC 1 cut(s) 744
PcsI WCGNNNNNNNCGW 1 cut(s) 265
PctI GAATGC 1 cut(s) 276
PfeI GAWTC 2 cut(s) 98, 738
PflFI GACNNNGTC 1 cut(s) 143
PkrI GCNGC 9 cut(s) 218, 221, 364, 385, 484, 487, 490, 499, 727
PspFI CCCAGC 1 cut(s) 530
PspN4I GGNNCC 1 cut(s) 248
PsrI GAACNNNNNNTAC 2 cut(s) 580, 612
PstNI CAGNNNCTG 1 cut(s) 734
PsuI RGATCY 1 cut(s) 649
PsyI GACNNNGTC 1 cut(s) 143
PvuII CAGCTG 1 cut(s) 353
RsaI GTAC 3 cut(s) 267, 577, 589
RsaNI GTAC 3 cut(s) 266, 576, 588
RseI CAYNNNNRTG 4 cut(s) 234, 709, 720, 760
SapI GCTCTTC 1 cut(s) 744
SaqAI TTAA 1 cut(s) 772
SatI GCNGC 9 cut(s) 217, 220, 363, 384, 483, 486, 489, 498, 726
Sau3AI GATC 5 cut(s) 105, 127, 226, 649, 679
ScaI AGTACT 1 cut(s) 577
SfaNI GCATC 2 cut(s) 19, 724
SmiMI CAYNNNNRTG 4 cut(s) 234, 709, 720, 760
SmlI CTYRAG 2 cut(s) 33, 148
SmoI CTYRAG 2 cut(s) 33, 148
SpeI ACTAGT 1 cut(s) 578
Sse9I AATT 6 cut(s) 133, 327, 418, 428, 632, 683
SsiI CCGC 1 cut(s) 304
SspMI CTAG 2 cut(s) 579, 746
StyI CCWWGG 1 cut(s) 468
TaqI TCGA 1 cut(s) 108
TasI AATT 6 cut(s) 133, 327, 418, 428, 632, 683
TatI WGTACW 2 cut(s) 575, 587
TfiI GAWTC 2 cut(s) 98, 738
Tru1I TTAA 1 cut(s) 772
Tru9I TTAA 1 cut(s) 772
TscAI CASTG 2 cut(s) 229, 531
TseI GCWGC 9 cut(s) 216, 219, 362, 383, 482, 485, 488, 497, 725
TspDTI ATGAA 4 cut(s) 555, 591, 646, 690
TspRI CASTG 2 cut(s) 229, 531
Tth111I GACNNNGTC 1 cut(s) 143
XapI RAATTY 3 cut(s) 133, 327, 418
XspI CTAG 2 cut(s) 579, 746
ZrmI AGTACT 1 cut(s) 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.