Rroxscaffold_4G00308600

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0012492
Physical Location & Seq
Forward (+)
30142133 .. 30142963
831 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_4G00308600.1

Sequence Viewer

Length: 831 bp
ATGGCTAAGAAAATCAACATAGAGAGTGATGCTCAAGCAAACCTCCACACCTCCTCTTTTTCGTCCTCTTCCTCCTCCTCCTCAACCTTGACAACCCATGATTCTTTGATCGATAAAAGCACAGAGAATGATCAGAATTCAGACAAGGTCTCAAGCTCAAAAGGGCAAGAGCTAAACAATGGGAGTAATGAGCCTAACACCAAGAAGAGGCAGAGGAGCAGCAACACTGATCATGGCGATGGAGGCAGGCACCCGACTTTTCGGGGAGTACGAATGCGCTCTTGGGGCAAATGGGTGTCCGAAATCCGCGAGCCGAGGAAGAAGTCAAGAATTTGGTTAGGGACATACCCAACAGCTGAAATGGCAGCCAGAGCGCACGATGTGGCCGCTCGGGCTATCAAAGGTGCTTCTGCTTATCTCAATTTCCCTGAATTGGCTCAAACGCTTCCACAACCGGCTTCTGCTTCTCCCAAGGACATTCAAGCTGCTGCTGCAAAAGCTGCTTCTGCTTTTGAGGAGGTGAAGAAATGTGAAGCTGAAATGGAAGAAGAAGAAGAAGAAGAAGAAGAAGAGGAAGCAACACTGCCGAGCAAAGATGAACAGACAACTACTACTGCTAATACTAGTATTAGTACTAGTAATAGTACAGCTTGTTCTTCATCTTCCACTTCAAATGATTATGATGACAAATTGTTTGACCTTCCAGATCTTTTCATGGATGGCACAAATGGGAGTGATCAATTTTGCTTTTCTTCTTCATCATCATCATGGCATCTGTGTGCAGCAGATACTGGATTCAGACTAGAAGAGCCATTCTTGTGGGAGTATTAA

Protein Analysis

276

Amino Acids

30.22

Weight (kDa)

4.84

Isoelectric Point (pI)

58.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 86 - 135 3.8e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012492)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44940
fragaria_vesca FvH4_7g09550
malus_domestica MD02G1217900.v1.1 MD07G1099400.v1.1
prunus_persica Prupe.2G123200_v2.0.a1
pyrus_communis pycom07g08150 pycom1310g00030
rosa_chinensis RchiOBHm_Chr1g0346421
rosa_laevigata RLG00000007751 RLG00000028801
rosa_multiflora Rmu_sc0003363.1_g000069 Rmu_sc0022454.1_g000001
rosa_roxburghii Rroxscaffold_4G00308600
rosa_rugosa Rorug01G0179600
rosa_samantha Rh1AG199100 Rh1BG164800 Rh1CG184100 Rh1DG195600
rosa_wichuraiana Rw1G016700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 249
AccBSI CCGCTC 1 cut(s) 389
AccII CGCG 1 cut(s) 309
AciI CCGC 2 cut(s) 307, 387
AcoI YGGCCR 1 cut(s) 384
AcsI RAATTY 2 cut(s) 136, 330
AdeI CACNNNGTG 1 cut(s) 382
AfaI GTAC 3 cut(s) 270, 634, 646
AfiI CCNNNNNNNGG 2 cut(s) 207, 433
AgsI TTSAA 2 cut(s) 482, 672
AhlI ACTAGT 2 cut(s) 623, 635
AjuI GAANNNNNNNTTGG 2 cut(s) 265, 297
AluBI AGCT 7 cut(s) 156, 172, 356, 485, 500, 536, 650
AluI AGCT 7 cut(s) 156, 172, 356, 485, 500, 536, 650
Alw26I GTCTC 1 cut(s) 154
AlwNI CAGNNNCTG 1 cut(s) 791
Ama87I CYCGRG 1 cut(s) 390
AoxI GGCC 1 cut(s) 384
ApeKI GCWGC 7 cut(s) 219, 365, 485, 488, 491, 500, 782
ApoI RAATTY 2 cut(s) 136, 330
ArsI GACNNNNNNTTYG 2 cut(s) 677, 709
AspLEI GCGC 2 cut(s) 279, 376
AsuHPI GGTGA 1 cut(s) 532
AvaI CYCGRG 1 cut(s) 390
BanI GGYRCC 1 cut(s) 249
BbvI GCAGC 7 cut(s) 231, 377, 472, 475, 478, 487, 794
BccI CCATC 2 cut(s) 233, 713
BclI TGATCA 3 cut(s) 130, 229, 736
BcoDI GTCTC 1 cut(s) 154
BcuI ACTAGT 2 cut(s) 623, 635
BfaI CTAG 3 cut(s) 624, 636, 803
BglI GCCNNNNNGGC 1 cut(s) 392
BglII AGATCT 1 cut(s) 706
BisI GCNGC 8 cut(s) 220, 366, 387, 486, 489, 492, 501, 783
BlsI GCNGC 8 cut(s) 221, 367, 388, 487, 490, 493, 502, 784
BmcAI AGTACT 1 cut(s) 634
BmeT110I CYCGRG 1 cut(s) 390
BmiI GGNNCC 1 cut(s) 251
BmsI GCATC 2 cut(s) 19, 781
BplI GAGNNNNNCTC 2 cut(s) 16, 48
BpuEI CTTGAG 2 cut(s) 18, 136
Bsa29I ATCGAT 1 cut(s) 111
BsaI GGTCTC 1 cut(s) 154
BsaJI CCNNGG 2 cut(s) 314, 471
Bsc4I CCNNNNNNNGG 2 cut(s) 207, 433
Bse118I RCCGGY 1 cut(s) 454
Bse1I ACTGG 1 cut(s) 796
BseCI ATCGAT 1 cut(s) 111
BseDI CCNNGG 2 cut(s) 314, 471
BseGI GGATG 1 cut(s) 724
BseLI CCNNNNNNNGG 2 cut(s) 207, 433
BseNI ACTGG 1 cut(s) 796
BseRI GAGGAG 6 cut(s) 43, 64, 67, 70, 229, 530
BseXI GCAGC 7 cut(s) 231, 377, 472, 475, 478, 487, 794
BsgI GTGCAG 1 cut(s) 801
Bsh1236I CGCG 1 cut(s) 309
BshFI GGCC 1 cut(s) 386
BshNI GGYRCC 1 cut(s) 249
BshVI ATCGAT 1 cut(s) 111
BsiHKCI CYCGRG 1 cut(s) 390
BsiSI CCGG 1 cut(s) 455
BslFI GGGAC 1 cut(s) 355
BslI CCNNNNNNNGG 2 cut(s) 207, 433
BsmAI GTCTC 1 cut(s) 154
BsmFI GGGAC 1 cut(s) 355
BsmI GAATGC 1 cut(s) 279
BsnI GGCC 1 cut(s) 386
Bso31I GGTCTC 1 cut(s) 154
BsoBI CYCGRG 1 cut(s) 390
Bsp143I GATC 5 cut(s) 108, 130, 229, 706, 736
BspACI CCGC 2 cut(s) 307, 387
BspANI GGCC 1 cut(s) 386
BspDI ATCGAT 1 cut(s) 111
BspFNI CGCG 1 cut(s) 309
BspLI GGNNCC 1 cut(s) 251
BspQI GCTCTTC 1 cut(s) 801
BspT107I GGYRCC 1 cut(s) 249
BspTNI GGTCTC 1 cut(s) 154
BsrBI CCGCTC 1 cut(s) 389
BsrFI RCCGGY 1 cut(s) 454
BsrI ACTGG 1 cut(s) 796
BssAI RCCGGY 1 cut(s) 454
BssECI CCNNGG 2 cut(s) 314, 471
BssMI GATC 5 cut(s) 108, 130, 229, 706, 736
BssT1I CCWWGG 1 cut(s) 471
Bst6I CTCTTC 4 cut(s) 73, 200, 564, 801
BstAPI GCANNNNNTGC 1 cut(s) 500
BstC8I GCNNGC 2 cut(s) 248, 311
BstDEI CTNAG 1 cut(s) 6
BstF5I GGATG 1 cut(s) 724
BstFNI CGCG 1 cut(s) 309
BstHHI GCGC 2 cut(s) 279, 376
BstKTI GATC 5 cut(s) 111, 133, 232, 709, 739
BstMAI GTCTC 1 cut(s) 154
BstMBI GATC 5 cut(s) 108, 130, 229, 706, 736
BstMWI GCNNNNNNNGC 9 cut(s) 243, 285, 362, 371, 392, 491, 497, 500, 506
BstUI CGCG 1 cut(s) 309
BstV1I GCAGC 7 cut(s) 231, 377, 472, 475, 478, 487, 794
BstX2I RGATCY 1 cut(s) 706
BstXI CCANNNNNNTGG 1 cut(s) 819
BstYI RGATCY 1 cut(s) 706
Bsu15I ATCGAT 1 cut(s) 111
BsuRI GGCC 1 cut(s) 386
BsuTUI ATCGAT 1 cut(s) 111
BtgZI GCGATG 1 cut(s) 252
BtsCI GGATG 1 cut(s) 724
BtsI GCAGTG 1 cut(s) 581
BtsIMutI CAGTG 2 cut(s) 225, 581
Cac8I GCNNGC 2 cut(s) 248, 311
CaiI CAGNNNCTG 1 cut(s) 791
CfoI GCGC 2 cut(s) 279, 376
Cfr10I RCCGGY 1 cut(s) 454
ClaI ATCGAT 1 cut(s) 111
Csp6I GTAC 3 cut(s) 269, 633, 645
CviAII CATG 4 cut(s) 98, 233, 715, 768
CviQI GTAC 3 cut(s) 269, 633, 645
DdeI CTNAG 1 cut(s) 6
DpnI GATC 5 cut(s) 110, 132, 231, 708, 738
DpnII GATC 5 cut(s) 108, 130, 229, 706, 736
DraIII CACNNNGTG 1 cut(s) 382
EaeI YGGCCR 1 cut(s) 384
Eam1104I CTCTTC 4 cut(s) 73, 200, 564, 801
EarI CTCTTC 4 cut(s) 73, 200, 564, 801
Eco130I CCWWGG 1 cut(s) 471
Eco31I GGTCTC 1 cut(s) 154
Eco88I CYCGRG 1 cut(s) 390
EcoRI GAATTC 1 cut(s) 136
EcoT14I CCWWGG 1 cut(s) 471
ErhI CCWWGG 1 cut(s) 471
FaeI CATG 4 cut(s) 101, 236, 718, 771
FaiI YATR 7 cut(s) 20, 99, 234, 346, 681, 716, 769
FaqI GGGAC 1 cut(s) 355
FatI CATG 4 cut(s) 97, 232, 714, 767
FbaI TGATCA 3 cut(s) 130, 229, 736
Fnu4HI GCNGC 8 cut(s) 220, 366, 387, 486, 489, 492, 501, 783
FokI GGATG 1 cut(s) 731
Fsp4HI GCNGC 8 cut(s) 220, 366, 387, 486, 489, 492, 501, 783
FspBI CTAG 3 cut(s) 624, 636, 803
GlaI GCGC 2 cut(s) 278, 375
GluI GCNGC 8 cut(s) 220, 366, 387, 486, 489, 492, 501, 783
HaeIII GGCC 1 cut(s) 386
HapII CCGG 1 cut(s) 455
HhaI GCGC 2 cut(s) 279, 376
Hin1II CATG 4 cut(s) 101, 236, 718, 771
Hin6I GCGC 2 cut(s) 277, 374
HinP1I GCGC 2 cut(s) 277, 374
HinfI GANTC 2 cut(s) 101, 795
HpaII CCGG 1 cut(s) 455
HphI GGTGA 1 cut(s) 532
Hpy188I TCNGA 4 cut(s) 135, 142, 301, 800
Hpy188III TCNNGA 2 cut(s) 327, 704
HpyAV CCTTC 1 cut(s) 710
HpyCH4V TGCA 2 cut(s) 494, 782
HpyF10VI GCNNNNNNNGC 9 cut(s) 243, 285, 362, 371, 392, 491, 497, 500, 506
HpyF3I CTNAG 1 cut(s) 6
Hsp92II CATG 4 cut(s) 101, 236, 718, 771
HspAI GCGC 2 cut(s) 277, 374
Ksp22I TGATCA 3 cut(s) 130, 229, 736
Kzo9I GATC 5 cut(s) 108, 130, 229, 706, 736
LguI GCTCTTC 1 cut(s) 801
LmnI GCTCC 1 cut(s) 216
LpnPI CCDG 6 cut(s) 232, 382, 441, 468, 717, 777
Lsp1109I GCAGC 7 cut(s) 231, 377, 472, 475, 478, 487, 794
LweI GCATC 2 cut(s) 19, 781
MaeI CTAG 3 cut(s) 624, 636, 803
MalI GATC 5 cut(s) 110, 132, 231, 708, 738
MbiI CCGCTC 1 cut(s) 389
MboI GATC 5 cut(s) 108, 130, 229, 706, 736
MflI RGATCY 1 cut(s) 706
MluCI AATT 6 cut(s) 136, 330, 421, 431, 689, 740
MseI TTAA 1 cut(s) 829
MslI CAYNNNNRTG 4 cut(s) 237, 766, 777, 817
MspA1I CMGCKG 1 cut(s) 356
MspI CCGG 1 cut(s) 455
Mva1269I GAATGC 1 cut(s) 279
MvnI CGCG 1 cut(s) 309
MwoI GCNNNNNNNGC 9 cut(s) 243, 285, 362, 371, 392, 491, 497, 500, 506
NdeII GATC 5 cut(s) 108, 130, 229, 706, 736
NlaIII CATG 4 cut(s) 101, 236, 718, 771
NlaIV GGNNCC 1 cut(s) 251
NmeAIII GCCGAG 2 cut(s) 339, 612
PciSI GCTCTTC 1 cut(s) 801
PcsI WCGNNNNNNNCGW 1 cut(s) 268
PctI GAATGC 1 cut(s) 279
PfeI GAWTC 2 cut(s) 101, 795
PflFI GACNNNGTC 1 cut(s) 146
PkrI GCNGC 8 cut(s) 221, 367, 388, 487, 490, 493, 502, 784
PspN4I GGNNCC 1 cut(s) 251
PsrI GAACNNNNNNTAC 2 cut(s) 637, 669
PstNI CAGNNNCTG 1 cut(s) 791
PsuI RGATCY 1 cut(s) 706
PsyI GACNNNGTC 1 cut(s) 146
PvuII CAGCTG 1 cut(s) 356
RsaI GTAC 3 cut(s) 270, 634, 646
RsaNI GTAC 3 cut(s) 269, 633, 645
RseI CAYNNNNRTG 4 cut(s) 237, 766, 777, 817
SapI GCTCTTC 1 cut(s) 801
SaqAI TTAA 1 cut(s) 829
SatI GCNGC 8 cut(s) 220, 366, 387, 486, 489, 492, 501, 783
Sau3AI GATC 5 cut(s) 108, 130, 229, 706, 736
ScaI AGTACT 1 cut(s) 634
SfaNI GCATC 2 cut(s) 19, 781
SmiMI CAYNNNNRTG 4 cut(s) 237, 766, 777, 817
SmlI CTYRAG 2 cut(s) 33, 151
SmoI CTYRAG 2 cut(s) 33, 151
SpeI ACTAGT 2 cut(s) 623, 635
Sse9I AATT 6 cut(s) 136, 330, 421, 431, 689, 740
SsiI CCGC 2 cut(s) 307, 387
SspMI CTAG 3 cut(s) 624, 636, 803
StyI CCWWGG 1 cut(s) 471
TaqI TCGA 1 cut(s) 111
TasI AATT 6 cut(s) 136, 330, 421, 431, 689, 740
TatI WGTACW 2 cut(s) 632, 644
TauI GCSGC 1 cut(s) 389
TfiI GAWTC 2 cut(s) 101, 795
Tru1I TTAA 1 cut(s) 829
Tru9I TTAA 1 cut(s) 829
TscAI CASTG 2 cut(s) 232, 588
TseI GCWGC 7 cut(s) 219, 365, 485, 488, 491, 500, 782
TspDTI ATGAA 4 cut(s) 612, 648, 703, 747
TspRI CASTG 2 cut(s) 232, 588
Tth111I GACNNNGTC 1 cut(s) 146
XapI RAATTY 2 cut(s) 136, 330
XspI CTAG 3 cut(s) 624, 636, 803
ZrmI AGTACT 1 cut(s) 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.