MD02G1217900.v1.1

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012492
Physical Location & Seq
Forward (+)
25320309 .. 25321145
837 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1217900.v1.1.491

Sequence Viewer

Length: 837 bp
ATGGCAAACCAAATCAACATAGAGAGTGATGCTCAAGAAAACCTTCTCCCCTCCTTCACCTCCACCACCTCCTCCTCTTCCTCCACCTCCTCCTCCTCCTCCACCTCTTCCTCCTCCACGATAACGACAAATTTCAATTCATTGACGGATCATAAAACCACACAAGTTTCTCTAAATTCAGATAATTGTACATCAAAAAAAGGGCAAAGGCCAAACAATGAGAAAGAGCAAGGCAACAAGAAGAAGAGGCAGAGGACTACTAGTGATCACAGTGATGGCAAGCACCCGACGTACCGCGGCGTAAGAATGCGCAGTTGGGGAAAATGGGTGTCGGAAATCCGCGAGCCGAGGAAGAAATCGAGAATATGGTTAGGGACATACCCAACTGCTGAAATGGCAGCCAGGGCTCATGATGTGGCAACTCTAGCCATCAAAGGTGTTTCTGCTTATCTCAATTTCCCTGAATTGGCTCAAGCACTTCCCCAGCCAGACTCCAAGTCTCCAAAGGACATCCAAGCTGCTGCTGCCAAGGCAGCTGCTGCTGCTGCTTCTGCTTCTGAGGATAAGCAGCTTATATGTGAAGCAGAAACTAAAGAAGCGGAGCCCAAGCTCCCTATATCAACAAATTCAACCTTGTCCAATGCTCAAGAATCAAGTAGCACTTGTGCTTCTGGTTCAACAGATTACGATGATGAGAAATTGTTTGACCTACCAGATCTTTTCATGGATGGGAAAGATGGAAGTGATAATTTTTGCAATTATTCTTCTTCAACTTGGCATTTGTGTGCAGCTGACACTGGATTCAGGCTAGGGGACCCATTCTTATGGGAGTATTAG

Protein Analysis

279

Amino Acids

30.25

Weight (kDa)

5.79

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 97 - 146 8.8e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012492)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44940
fragaria_vesca FvH4_7g09550
malus_domestica MD02G1217900.v1.1 MD07G1099400.v1.1
prunus_persica Prupe.2G123200_v2.0.a1
pyrus_communis pycom07g08150 pycom1310g00030
rosa_chinensis RchiOBHm_Chr1g0346421
rosa_laevigata RLG00000007751 RLG00000028801
rosa_multiflora Rmu_sc0003363.1_g000069 Rmu_sc0022454.1_g000001
rosa_roxburghii Rroxscaffold_4G00308600
rosa_rugosa Rorug01G0179600
rosa_samantha Rh1AG199100 Rh1BG164800 Rh1CG184100 Rh1DG195600
rosa_wichuraiana Rw1G016700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 311
AccII CGCG 2 cut(s) 297, 342
AciI CCGC 4 cut(s) 295, 297, 340, 599
AclWI GGATC 1 cut(s) 156
AcsI RAATTY 3 cut(s) 130, 175, 625
AfaI GTAC 2 cut(s) 190, 293
AfiI CCNNNNNNNGG 1 cut(s) 466
AgsI TTSAA 4 cut(s) 136, 630, 678, 771
AhdI GACNNNNNGTC 1 cut(s) 496
AhlI ACTAGT 1 cut(s) 260
AjnI CCWGG 1 cut(s) 401
AjuI GAANNNNNNNTTGG 2 cut(s) 298, 330
AluBI AGCT 5 cut(s) 518, 536, 571, 610, 791
AluI AGCT 5 cut(s) 518, 536, 571, 610, 791
Alw26I GTCTC 1 cut(s) 504
AlwI GGATC 1 cut(s) 156
AlwNI CAGNNNCTG 1 cut(s) 539
AoxI GGCC 1 cut(s) 209
ApoI RAATTY 3 cut(s) 130, 175, 625
Asp700I GAANNNNTTC 1 cut(s) 42
AspLEI GCGC 1 cut(s) 312
AspS9I GGNCC 1 cut(s) 814
AsuHPI GGTGA 1 cut(s) 49
AvaII GGWCC 1 cut(s) 814
BanII GRGCYC 2 cut(s) 409, 606
BccI CCATC 4 cut(s) 269, 437, 722, 731
BciT130I CCWGG 1 cut(s) 403
BclI TGATCA 1 cut(s) 265
BcoDI GTCTC 1 cut(s) 504
BcuI ACTAGT 1 cut(s) 260
BfaI CTAG 3 cut(s) 261, 425, 809
BglII AGATCT 1 cut(s) 715
Bme1390I CCNGG 1 cut(s) 403
Bme18I GGWCC 1 cut(s) 814
BmeRI GACNNNNNGTC 1 cut(s) 496
BmgT120I GGNCC 1 cut(s) 814
BmiI GGNNCC 3 cut(s) 603, 815, 816
BmrFI CCNGG 1 cut(s) 403
BmsI GCATC 1 cut(s) 19
BplI GAGNNNNNCTC 2 cut(s) 16, 48
BpuEI CTTGAG 3 cut(s) 18, 456, 630
BsaJI CCNNGG 4 cut(s) 295, 347, 402, 528
Bsc4I CCNNNNNNNGG 1 cut(s) 466
Bse1I ACTGG 1 cut(s) 802
BseBI CCWGG 1 cut(s) 403
BseDI CCNNGG 4 cut(s) 295, 347, 402, 528
BseGI GGATG 2 cut(s) 510, 733
BseLI CCNNNNNNNGG 1 cut(s) 466
BseMII CTCAG 1 cut(s) 549
BseNI ACTGG 1 cut(s) 802
BseRI GAGGAG 7 cut(s) 61, 64, 79, 82, 85, 88, 103
BseYI CCCAGC 1 cut(s) 483
BsgI GTGCAG 1 cut(s) 807
Bsh1236I CGCG 2 cut(s) 297, 342
BshFI GGCC 1 cut(s) 211
BslFI GGGAC 2 cut(s) 388, 827
BslI CCNNNNNNNGG 1 cut(s) 466
BsmAI GTCTC 1 cut(s) 504
BsmFI GGGAC 2 cut(s) 388, 827
BsmI GAATGC 1 cut(s) 312
BsnI GGCC 1 cut(s) 211
Bsp1286I GDGCHC 2 cut(s) 409, 606
Bsp1407I TGTACA 1 cut(s) 188
Bsp143I GATC 3 cut(s) 148, 265, 715
BspACI CCGC 4 cut(s) 295, 297, 340, 599
BspANI GGCC 1 cut(s) 211
BspCNI CTCAG 1 cut(s) 550
BspFNI CGCG 2 cut(s) 297, 342
BspHI TCATGA 1 cut(s) 409
BspLI GGNNCC 3 cut(s) 603, 815, 816
BspPI GGATC 1 cut(s) 156
BsrGI TGTACA 1 cut(s) 188
BsrI ACTGG 1 cut(s) 802
BssECI CCNNGG 4 cut(s) 295, 347, 402, 528
BssMI GATC 3 cut(s) 148, 265, 715
BssT1I CCWWGG 1 cut(s) 528
Bst2UI CCWGG 1 cut(s) 403
Bst4CI ACNGT 1 cut(s) 272
Bst6I CTCTTC 3 cut(s) 82, 112, 239
BstAPI GCANNNNNTGC 1 cut(s) 539
BstAUI TGTACA 1 cut(s) 188
BstC8I GCNNGC 2 cut(s) 281, 344
BstDEI CTNAG 1 cut(s) 558
BstDSI CCRYGG 1 cut(s) 295
BstF5I GGATG 2 cut(s) 510, 733
BstFNI CGCG 2 cut(s) 297, 342
BstHHI GCGC 1 cut(s) 312
BstKTI GATC 3 cut(s) 151, 268, 718
BstMAI GTCTC 1 cut(s) 504
BstMBI GATC 3 cut(s) 148, 265, 715
BstNI CCWGG 1 cut(s) 403
BstSCI CCNGG 1 cut(s) 401
BstUI CGCG 2 cut(s) 297, 342
BstX2I RGATCY 1 cut(s) 715
BstXI CCANNNNNNTGG 1 cut(s) 825
BstYI RGATCY 1 cut(s) 715
BsuRI GGCC 1 cut(s) 211
BtgI CCRYGG 1 cut(s) 295
BtsCI GGATG 2 cut(s) 510, 733
BtsIMutI CAGTG 2 cut(s) 277, 795
Cac8I GCNNGC 2 cut(s) 281, 344
CaiI CAGNNNCTG 1 cut(s) 539
CciI TCATGA 1 cut(s) 409
CfoI GCGC 1 cut(s) 312
Cfr13I GGNCC 1 cut(s) 814
Cfr42I CCGCGG 1 cut(s) 298
Csp6I GTAC 2 cut(s) 189, 292
CviAII CATG 2 cut(s) 410, 724
CviQI GTAC 2 cut(s) 189, 292
DdeI CTNAG 1 cut(s) 558
DpnI GATC 3 cut(s) 150, 267, 717
DpnII GATC 3 cut(s) 148, 265, 715
DriI GACNNNNNGTC 1 cut(s) 496
Eam1104I CTCTTC 3 cut(s) 82, 112, 239
Eam1105I GACNNNNNGTC 1 cut(s) 496
EarI CTCTTC 3 cut(s) 82, 112, 239
Eco130I CCWWGG 1 cut(s) 528
Eco24I GRGCYC 2 cut(s) 409, 606
Eco47I GGWCC 1 cut(s) 814
EcoO109I RGGNCCY 1 cut(s) 814
EcoRII CCWGG 1 cut(s) 401
EcoT14I CCWWGG 1 cut(s) 528
EcoT38I GRGCYC 2 cut(s) 409, 606
ErhI CCWWGG 1 cut(s) 528
FaeI CATG 2 cut(s) 413, 727
FalI AAGNNNNNCTT 4 cut(s) 27, 59, 646, 678
FaqI GGGAC 2 cut(s) 388, 827
FatI CATG 2 cut(s) 409, 723
FbaI TGATCA 1 cut(s) 265
FokI GGATG 2 cut(s) 497, 740
FriOI GRGCYC 2 cut(s) 409, 606
FspBI CTAG 3 cut(s) 261, 425, 809
FspI TGCGCA 1 cut(s) 311
GlaI GCGC 1 cut(s) 311
GsaI CCCAGC 1 cut(s) 487
HaeIII GGCC 1 cut(s) 211
HhaI GCGC 1 cut(s) 312
Hin1II CATG 2 cut(s) 413, 727
Hin6I GCGC 1 cut(s) 310
HinP1I GCGC 1 cut(s) 310
HinfI GANTC 3 cut(s) 491, 650, 801
HphI GGTGA 1 cut(s) 49
Hpy188I TCNGA 3 cut(s) 181, 334, 559
Hpy188III TCNNGA 4 cut(s) 35, 360, 410, 647
Hpy99I CGWCG 1 cut(s) 292
HpyAV CCTTC 2 cut(s) 53, 64
HpyCH4III ACNGT 1 cut(s) 272
HpyCH4IV ACGT 1 cut(s) 290
HpyCH4V TGCA 2 cut(s) 756, 788
HpyF3I CTNAG 1 cut(s) 558
HpySE526I ACGT 1 cut(s) 290
Hsp92II CATG 2 cut(s) 413, 727
HspAI GCGC 1 cut(s) 310
KflI GGGWCCC 1 cut(s) 814
Ksp22I TGATCA 1 cut(s) 265
KspI CCGCGG 1 cut(s) 298
Kzo9I GATC 3 cut(s) 148, 265, 715
LmnI GCTCC 2 cut(s) 601, 615
LpnPI CCDG 9 cut(s) 388, 415, 474, 497, 501, 657, 726, 783, 790
LweI GCATC 1 cut(s) 19
MaeI CTAG 3 cut(s) 261, 425, 809
MaeII ACGT 1 cut(s) 290
MalI GATC 3 cut(s) 150, 267, 717
MboI GATC 3 cut(s) 148, 265, 715
MboII GAAGA 7 cut(s) 69, 99, 253, 256, 364, 756, 759
MflI RGATCY 1 cut(s) 715
MhlI GDGCHC 2 cut(s) 409, 606
MlyI GAGTC 1 cut(s) 485
MmeI TCCRAC 1 cut(s) 312
MroXI GAANNNNTTC 1 cut(s) 42
MslI CAYNNNNRTG 3 cut(s) 273, 783, 823
MspA1I CMGCKG 3 cut(s) 297, 536, 791
MspR9I CCNGG 1 cut(s) 403
Mva1269I GAATGC 1 cut(s) 312
MvaI CCWGG 1 cut(s) 403
MvnI CGCG 2 cut(s) 297, 342
NdeII GATC 3 cut(s) 148, 265, 715
NlaIII CATG 2 cut(s) 413, 727
NlaIV GGNNCC 3 cut(s) 603, 815, 816
NmeAIII GCCGAG 1 cut(s) 372
NsbI TGCGCA 1 cut(s) 311
PagI TCATGA 1 cut(s) 409
PctI GAATGC 1 cut(s) 312
PdmI GAANNNNTTC 1 cut(s) 42
PfeI GAWTC 2 cut(s) 650, 801
PleI GAGTC 1 cut(s) 485
PpsI GAGTC 1 cut(s) 485
PpuMI RGGWCCY 1 cut(s) 814
Psp5II RGGWCCY 1 cut(s) 814
Psp6I CCWGG 1 cut(s) 401
PspFI CCCAGC 1 cut(s) 483
PspGI CCWGG 1 cut(s) 401
PspN4I GGNNCC 3 cut(s) 603, 815, 816
PspPI GGNCC 1 cut(s) 814
PspPPI RGGWCCY 1 cut(s) 814
PstNI CAGNNNCTG 1 cut(s) 539
PsuI RGATCY 1 cut(s) 715
PvuII CAGCTG 2 cut(s) 536, 791
RsaI GTAC 2 cut(s) 190, 293
RsaNI GTAC 2 cut(s) 189, 292
RseI CAYNNNNRTG 3 cut(s) 273, 783, 823
SacII CCGCGG 1 cut(s) 298
Sau3AI GATC 3 cut(s) 148, 265, 715
Sau96I GGNCC 1 cut(s) 814
SchI GAGTC 1 cut(s) 485
ScrFI CCNGG 1 cut(s) 403
SduI GDGCHC 2 cut(s) 409, 606
SfaNI GCATC 1 cut(s) 19
Sfr303I CCGCGG 1 cut(s) 298
SgrBI CCGCGG 1 cut(s) 298
SinI GGWCC 1 cut(s) 814
SmiMI CAYNNNNRTG 3 cut(s) 273, 783, 823
SmlI CTYRAG 3 cut(s) 33, 471, 645
SmoI CTYRAG 3 cut(s) 33, 471, 645
SpeI ACTAGT 1 cut(s) 260
SsiI CCGC 4 cut(s) 295, 297, 340, 599
SspMI CTAG 3 cut(s) 261, 425, 809
StyD4I CCNGG 1 cut(s) 401
StyI CCWWGG 1 cut(s) 528
TaaI ACNGT 1 cut(s) 272
TaiI ACGT 1 cut(s) 293
TaqI TCGA 1 cut(s) 359
TatI WGTACW 1 cut(s) 188
TauI GCSGC 1 cut(s) 300
TfiI GAWTC 2 cut(s) 650, 801
TscAI CASTG 2 cut(s) 277, 802
TspDTI ATGAA 2 cut(s) 129, 712
TspGWI ACGGA 1 cut(s) 161
TspRI CASTG 2 cut(s) 277, 802
VpaK11BI GGWCC 1 cut(s) 814
XapI RAATTY 3 cut(s) 130, 175, 625
XmnI GAANNNNTTC 1 cut(s) 42
XspI CTAG 3 cut(s) 261, 425, 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.