pycom07g08150

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0012492
Physical Location & Seq
Reverse (-)
7521313 .. 7522188
876 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g08150.1

Sequence Viewer

Length: 876 bp
ATGTTTTTTTGCACCACTCACATTTGTTTCTGTAAAACTCTGGAGCTAGTTAGGCCCCCTATCATGGCAAACCAAATCAACATAGAGAGTGATGCTCAAGAAAACCTCCTCACCACCACTACCACCTGCTCCTCCTCCTCCTCTTCCTCCTCCTCCACGATAACGAATTCCCATTCATTGGCGGATCATAAAAGCACACGAGTTTCTCTATCAGAGAATTGTACTCCAAAAAAAGGGCAAGAGCCAAACAATGAGAAAGAGCAAGGCAACAACAAGAAGAGGCGGAGGACAACTAGTGATGACAGTAATGGCAAGCACCCGACTTTCCGCGGCGTACGAATGCGCAGTTGGGGAAAATGGGTGTCGGAAATTCGCGAGCCGAGGAAGAAATCAAGAATATGGTTAGGGACGTACCAAACAGCTGAAATGGCAGCTAGAGCTCATGATGTGGCAGCTCTAGCCATCAAAGGTGCTTCTGCTTATCTCAATTTCCCTGAACTGGCCCAAGCACTTCCCCAGCCAAACTCCAAGTCTCCAAAGGACATCCAAGCTGCAGCTGCCAAGGCAGCAGCAGAAGCAGCATCTGCTTCTGCTTCTGAGGAAAAGCAGCTTATTTGTGAAGCAGAAACTAAAGAAGCAGAGGCCAAGCTCCCCATGTCAGCACATTCAACTTTGTCCAATGCTCAAGAATCAAGTAGCACTTGTGCTTCTGCTTCAACAGATTATGATGAGGAGCAATTGTTTGACCTACCAGATCTTGTCATGGATGGGAAAGATGGAAGCGATAATTTTTGCTATTATTCTTCTTCAACTTGGCATTTGTGTGCAGCTGACACTGGATTCAGGCTAGAGGAGCCATTCTTGTGGGAGTATTAG

Protein Analysis

292

Amino Acids

31.98

Weight (kDa)

5.72

Isoelectric Point (pI)

52.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 108 - 157 6.9e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012492)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44940
fragaria_vesca FvH4_7g09550
malus_domestica MD02G1217900.v1.1 MD07G1099400.v1.1
prunus_persica Prupe.2G123200_v2.0.a1
pyrus_communis pycom07g08150 pycom1310g00030
rosa_chinensis RchiOBHm_Chr1g0346421
rosa_laevigata RLG00000007751 RLG00000028801
rosa_multiflora Rmu_sc0003363.1_g000069 Rmu_sc0022454.1_g000001
rosa_roxburghii Rroxscaffold_4G00308600
rosa_rugosa Rorug01G0179600
rosa_samantha Rh1AG199100 Rh1BG164800 Rh1CG184100 Rh1DG195600
rosa_wichuraiana Rw1G016700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 134
Acc16I TGCGCA 1 cut(s) 344
Acc36I ACCTGC 1 cut(s) 134
AccB7I CCANNNNNTGG 1 cut(s) 178
AccII CGCG 2 cut(s) 330, 375
AciI CCGC 4 cut(s) 182, 283, 328, 330
AclWI GGATC 1 cut(s) 192
AcsI RAATTY 2 cut(s) 166, 369
AfaI GTAC 3 cut(s) 223, 336, 413
AfiI CCNNNNNNNGG 4 cut(s) 64, 178, 233, 499
AgsI TTSAA 3 cut(s) 669, 717, 810
AhlI ACTAGT 1 cut(s) 293
AjuI GAANNNNNNNTTGG 2 cut(s) 331, 363
Alw21I GWGCWC 1 cut(s) 442
Alw26I GTCTC 1 cut(s) 537
AlwI GGATC 1 cut(s) 192
AlwNI CAGNNNCTG 1 cut(s) 584
AoxI GGCC 3 cut(s) 53, 501, 642
ApoI RAATTY 2 cut(s) 166, 369
ArsI GACNNNNNNTTYG 2 cut(s) 515, 547
AspLEI GCGC 1 cut(s) 345
AspS9I GGNCC 2 cut(s) 54, 502
AsuHPI GGTGA 1 cut(s) 103
BanII GRGCYC 1 cut(s) 442
BauI CACGAG 1 cut(s) 198
Bbv12I GWGCWC 1 cut(s) 442
BccI CCATC 3 cut(s) 470, 761, 770
BcoDI GTCTC 1 cut(s) 537
BcuI ACTAGT 1 cut(s) 293
BfaI CTAG 5 cut(s) 47, 294, 435, 458, 848
BfmI CTRYAG 1 cut(s) 552
BfuAI ACCTGC 1 cut(s) 134
BglII AGATCT 1 cut(s) 754
BmgT120I GGNCC 2 cut(s) 54, 502
BmiI GGNNCC 2 cut(s) 56, 855
BmsI GCATC 2 cut(s) 82, 590
BplI GAGNNNNNCTC 2 cut(s) 79, 111
BpmI CTGGAG 1 cut(s) 62
BpuEI CTTGAG 2 cut(s) 81, 669
BsaJI CCNNGG 3 cut(s) 328, 380, 561
BsaXI ACNNNNNCTCC 2 cut(s) 725, 755
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 178, 233, 499
Bse1I ACTGG 2 cut(s) 504, 841
BseDI CCNNGG 3 cut(s) 328, 380, 561
BseGI GGATG 2 cut(s) 543, 772
BseLI CCNNNNNNNGG 4 cut(s) 64, 178, 233, 499
BseMII CTCAG 1 cut(s) 588
BseNI ACTGG 2 cut(s) 504, 841
BseRI GAGGAG 9 cut(s) 98, 121, 124, 127, 130, 139, 142, 746, 866
BseYI CCCAGC 1 cut(s) 516
BsgI GTGCAG 1 cut(s) 846
Bsh1236I CGCG 2 cut(s) 330, 375
BshFI GGCC 3 cut(s) 55, 503, 644
BsiHKAI GWGCWC 1 cut(s) 442
BsiWI CGTACG 1 cut(s) 334
BslFI GGGAC 1 cut(s) 421
BslI CCNNNNNNNGG 4 cut(s) 64, 178, 233, 499
BsmAI GTCTC 1 cut(s) 537
BsmFI GGGAC 1 cut(s) 421
BsmI GAATGC 1 cut(s) 345
BsnI GGCC 3 cut(s) 55, 503, 644
Bsp1286I GDGCHC 1 cut(s) 442
Bsp143I GATC 2 cut(s) 184, 754
Bsp68I TCGCGA 1 cut(s) 375
BspACI CCGC 4 cut(s) 182, 283, 328, 330
BspANI GGCC 3 cut(s) 55, 503, 644
BspCNI CTCAG 1 cut(s) 589
BspFNI CGCG 2 cut(s) 330, 375
BspHI TCATGA 1 cut(s) 442
BspLI GGNNCC 2 cut(s) 56, 855
BspMAI CTGCAG 1 cut(s) 556
BspMI ACCTGC 1 cut(s) 134
BspPI GGATC 1 cut(s) 192
BsrI ACTGG 2 cut(s) 504, 841
BssECI CCNNGG 3 cut(s) 328, 380, 561
BssMI GATC 2 cut(s) 184, 754
BssSI CACGAG 1 cut(s) 198
BssT1I CCWWGG 1 cut(s) 561
Bst2BI CACGAG 1 cut(s) 198
Bst4CI ACNGT 1 cut(s) 305
Bst6I CTCTTC 2 cut(s) 148, 272
BstAPI GCANNNNNTGC 1 cut(s) 584
BstC8I GCNNGC 2 cut(s) 314, 377
BstDEI CTNAG 1 cut(s) 597
BstDSI CCRYGG 1 cut(s) 328
BstF5I GGATG 2 cut(s) 543, 772
BstFNI CGCG 2 cut(s) 330, 375
BstHHI GCGC 1 cut(s) 345
BstKTI GATC 2 cut(s) 187, 757
BstMAI GTCTC 1 cut(s) 537
BstMBI GATC 2 cut(s) 184, 754
BstSFI CTRYAG 1 cut(s) 552
BstUI CGCG 2 cut(s) 330, 375
BstX2I RGATCY 1 cut(s) 754
BstXI CCANNNNNNTGG 1 cut(s) 864
BstYI RGATCY 1 cut(s) 754
BsuRI GGCC 3 cut(s) 55, 503, 644
BtgI CCRYGG 1 cut(s) 328
BtsCI GGATG 2 cut(s) 543, 772
BtsIMutI CAGTG 1 cut(s) 834
BtuMI TCGCGA 1 cut(s) 375
BveI ACCTGC 1 cut(s) 134
Cac8I GCNNGC 2 cut(s) 314, 377
CaiI CAGNNNCTG 1 cut(s) 584
CciI TCATGA 1 cut(s) 442
CfoI GCGC 1 cut(s) 345
Cfr13I GGNCC 2 cut(s) 54, 502
Cfr42I CCGCGG 1 cut(s) 331
Csp6I GTAC 3 cut(s) 222, 335, 412
CviAII CATG 4 cut(s) 64, 443, 655, 763
CviQI GTAC 3 cut(s) 222, 335, 412
DdeI CTNAG 1 cut(s) 597
DpnI GATC 2 cut(s) 186, 756
DpnII GATC 2 cut(s) 184, 754
Eam1104I CTCTTC 2 cut(s) 148, 272
EarI CTCTTC 2 cut(s) 148, 272
EciI GGCGGA 2 cut(s) 197, 298
Ecl136II GAGCTC 1 cut(s) 440
Eco130I CCWWGG 1 cut(s) 561
Eco24I GRGCYC 1 cut(s) 442
Eco53kI GAGCTC 1 cut(s) 440
EcoICRI GAGCTC 1 cut(s) 440
EcoO109I RGGNCCY 1 cut(s) 54
EcoRI GAATTC 1 cut(s) 166
EcoT14I CCWWGG 1 cut(s) 561
EcoT38I GRGCYC 1 cut(s) 442
ErhI CCWWGG 1 cut(s) 561
FaeI CATG 4 cut(s) 67, 446, 658, 766
FaiI YATR 8 cut(s) 65, 83, 189, 400, 444, 656, 726, 764
FalI AAGNNNNNCTT 2 cut(s) 685, 717
FaqI GGGAC 1 cut(s) 421
FatI CATG 4 cut(s) 63, 442, 654, 762
FokI GGATG 2 cut(s) 530, 779
FriOI GRGCYC 1 cut(s) 442
FspBI CTAG 5 cut(s) 47, 294, 435, 458, 848
FspI TGCGCA 1 cut(s) 344
GlaI GCGC 1 cut(s) 344
GsaI CCCAGC 1 cut(s) 520
GsuI CTGGAG 1 cut(s) 62
HaeIII GGCC 3 cut(s) 55, 503, 644
HhaI GCGC 1 cut(s) 345
Hin1II CATG 4 cut(s) 67, 446, 658, 766
Hin6I GCGC 1 cut(s) 343
HinP1I GCGC 1 cut(s) 343
HinfI GANTC 2 cut(s) 689, 840
HphI GGTGA 1 cut(s) 103
Hpy188I TCNGA 3 cut(s) 214, 367, 598
Hpy188III TCNNGA 6 cut(s) 41, 98, 374, 393, 443, 686
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4IV ACGT 1 cut(s) 410
HpyCH4V TGCA 3 cut(s) 12, 554, 827
HpyF3I CTNAG 1 cut(s) 597
HpySE526I ACGT 1 cut(s) 410
Hsp92II CATG 4 cut(s) 67, 446, 658, 766
HspAI GCGC 1 cut(s) 343
KspI CCGCGG 1 cut(s) 331
Kzo9I GATC 2 cut(s) 184, 754
LmnI GCTCC 5 cut(s) 43, 134, 654, 733, 853
LpnPI CCDG 8 cut(s) 26, 139, 485, 507, 530, 765, 822, 829
LweI GCATC 2 cut(s) 82, 590
MaeI CTAG 5 cut(s) 47, 294, 435, 458, 848
MaeII ACGT 1 cut(s) 410
MalI GATC 2 cut(s) 186, 756
MboI GATC 2 cut(s) 184, 754
MboII GAAGA 5 cut(s) 135, 289, 397, 795, 798
MfeI CAATTG 1 cut(s) 737
MflI RGATCY 1 cut(s) 754
MhlI GDGCHC 1 cut(s) 442
MluCI AATT 6 cut(s) 166, 217, 369, 487, 737, 787
MmeI TCCRAC 1 cut(s) 345
MslI CAYNNNNRTG 2 cut(s) 822, 862
MspA1I CMGCKG 4 cut(s) 330, 422, 557, 830
MunI CAATTG 1 cut(s) 737
Mva1269I GAATGC 1 cut(s) 345
MvnI CGCG 2 cut(s) 330, 375
NdeII GATC 2 cut(s) 184, 754
NlaIII CATG 4 cut(s) 67, 446, 658, 766
NlaIV GGNNCC 2 cut(s) 56, 855
NmeAIII GCCGAG 1 cut(s) 405
NruI TCGCGA 1 cut(s) 375
NsbI TGCGCA 1 cut(s) 344
PagI TCATGA 1 cut(s) 442
PaqCI CACCTGC 1 cut(s) 134
PctI GAATGC 1 cut(s) 345
PfeI GAWTC 2 cut(s) 689, 840
Pfl23II CGTACG 1 cut(s) 334
PflMI CCANNNNNTGG 1 cut(s) 178
Psp124BI GAGCTC 1 cut(s) 442
PspFI CCCAGC 1 cut(s) 516
PspLI CGTACG 1 cut(s) 334
PspN4I GGNNCC 2 cut(s) 56, 855
PspPI GGNCC 2 cut(s) 54, 502
PstI CTGCAG 1 cut(s) 556
PstNI CAGNNNCTG 1 cut(s) 584
PsuI RGATCY 1 cut(s) 754
PvuII CAGCTG 3 cut(s) 422, 557, 830
RruI TCGCGA 1 cut(s) 375
RsaI GTAC 3 cut(s) 223, 336, 413
RsaNI GTAC 3 cut(s) 222, 335, 412
RseI CAYNNNNRTG 2 cut(s) 822, 862
SacI GAGCTC 1 cut(s) 442
SacII CCGCGG 1 cut(s) 331
Sau3AI GATC 2 cut(s) 184, 754
Sau96I GGNCC 2 cut(s) 54, 502
SduI GDGCHC 1 cut(s) 442
SfaNI GCATC 2 cut(s) 82, 590
SfcI CTRYAG 1 cut(s) 552
Sfr303I CCGCGG 1 cut(s) 331
SgrBI CCGCGG 1 cut(s) 331
SmiMI CAYNNNNRTG 2 cut(s) 822, 862
SmlI CTYRAG 2 cut(s) 96, 684
SmoI CTYRAG 2 cut(s) 96, 684
SpeI ACTAGT 1 cut(s) 293
Sse9I AATT 6 cut(s) 166, 217, 369, 487, 737, 787
SsiI CCGC 4 cut(s) 182, 283, 328, 330
SspMI CTAG 5 cut(s) 47, 294, 435, 458, 848
SstI GAGCTC 1 cut(s) 442
StyI CCWWGG 1 cut(s) 561
TaaI ACNGT 1 cut(s) 305
TaiI ACGT 1 cut(s) 413
TasI AATT 6 cut(s) 166, 217, 369, 487, 737, 787
TatI WGTACW 1 cut(s) 221
TauI GCSGC 1 cut(s) 333
TfiI GAWTC 2 cut(s) 689, 840
TscAI CASTG 1 cut(s) 841
TspDTI ATGAA 1 cut(s) 165
TspRI CASTG 1 cut(s) 841
Van91I CCANNNNNTGG 1 cut(s) 178
XapI RAATTY 2 cut(s) 166, 369
XspI CTAG 5 cut(s) 47, 294, 435, 458, 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.