RchiOBHm_Chr1g0346421

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0012492
Physical Location & Seq
Reverse (-)
38777696 .. 38778970
1275 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57269

Sequence Viewer

Length: 834 bp
ATGGCTAAGAAAATCAACATAGAGAGTGATGCTCAAGCAAACCTCCACACCTCCTCTTCTTCCTCCTCCTCCTCCTCCTCCTCCTCCTCAACCTTGACAACCCATGATTCTTTGATCGATAAAAGCACAGAGAATGATCAGAATTCAGACAAGGTCTCAAGCTCAAAAGGGCAAGAGCTGAACAATGGGAGTAATGAGCCTAACACCAAGAAGAGGCTGAGGCAGAGGAGCAGCAACACTGATCATGGCGATGGAGGCAGGCACCCGACTTTTCGGGGAGTACGAATGCGCTCTTGGGGCAAATGGGTGTCCGAAATCCGCGAGCCGAGGAAGAAGTCCAGAATTTGGTTAGGGACATACCCAACAGCTGAAATGGCAGCCCGAGCGCACGATGTGGCTGCTCGGGCTATCAAAGGTGCCTCTGCTTATCTAAATTTCCCTGAATTGGCTCAAACGCTTCCACAACCGGCTTCTGCTTCTCCCAAGGACATTCAAGCTGCTGCTGCAAAAGCTGCTTCTGCTTTTGAGGAGGTGAAGAAATGTGAAGCTGAAACGGAAGAAGAAGAAGAAGAAGAGGAAGCAACACTGCCAAGCAAAGATGAACAGACAACTACTACTGCTAATACTAGTATCAGTACTAGTAATAGTACAGCTTGTTCTTCATCTTCCACTTCAAATGATTATGATGACAAATTGTTTGACCTTCCAGATCTTTTCATGGATGGCACAAATGGAAGTGATCAATTTTGCTTTTCTTCTTCATCATCATCATGGCATCTGTGTGCAGCAGATACTGGATTCAGACTAGAAGAGTCATTCTTGTGGGAGTATTAA

Protein Analysis

277

Amino Acids

30.18

Weight (kDa)

5.04

Isoelectric Point (pI)

58.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 90 - 139 3.8e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012492)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44940
fragaria_vesca FvH4_7g09550
malus_domestica MD02G1217900.v1.1 MD07G1099400.v1.1
prunus_persica Prupe.2G123200_v2.0.a1
pyrus_communis pycom07g08150 pycom1310g00030
rosa_chinensis RchiOBHm_Chr1g0346421
rosa_laevigata RLG00000007751 RLG00000028801
rosa_multiflora Rmu_sc0003363.1_g000069 Rmu_sc0022454.1_g000001
rosa_roxburghii Rroxscaffold_4G00308600
rosa_rugosa Rorug01G0179600
rosa_samantha Rh1AG199100 Rh1BG164800 Rh1CG184100 Rh1DG195600
rosa_wichuraiana Rw1G016700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 261, 416
AccB7I CCANNNNNTGG 1 cut(s) 345
AccII CGCG 1 cut(s) 321
AciI CCGC 1 cut(s) 319
AcsI RAATTY 3 cut(s) 142, 342, 433
AdeI CACNNNGTG 1 cut(s) 394
AfaI GTAC 3 cut(s) 282, 637, 649
AfiI CCNNNNNNNGG 3 cut(s) 213, 345, 445
AgsI TTSAA 2 cut(s) 494, 675
AhlI ACTAGT 2 cut(s) 626, 638
AjuI GAANNNNNNNTTGG 2 cut(s) 277, 309
AluBI AGCT 7 cut(s) 162, 178, 368, 497, 512, 548, 653
AluI AGCT 7 cut(s) 162, 178, 368, 497, 512, 548, 653
Alw26I GTCTC 1 cut(s) 160
AlwNI CAGNNNCTG 1 cut(s) 794
Ama87I CYCGRG 2 cut(s) 381, 402
ApeKI GCWGC 8 cut(s) 231, 377, 398, 497, 500, 503, 512, 785
ApoI RAATTY 3 cut(s) 142, 342, 433
ArsI GACNNNNNNTTYG 2 cut(s) 680, 712
AspLEI GCGC 2 cut(s) 291, 388
AsuHPI GGTGA 1 cut(s) 544
AvaI CYCGRG 2 cut(s) 381, 402
BanI GGYRCC 2 cut(s) 261, 416
BbvCI CCTCAGC 1 cut(s) 218
BbvI GCAGC 8 cut(s) 243, 385, 389, 484, 487, 490, 499, 797
BccI CCATC 2 cut(s) 245, 716
BcgI CGANNNNNNTGC 2 cut(s) 380, 414
BclI TGATCA 3 cut(s) 136, 241, 739
BcoDI GTCTC 1 cut(s) 160
BcuI ACTAGT 2 cut(s) 626, 638
BfaI CTAG 3 cut(s) 627, 639, 806
BglII AGATCT 1 cut(s) 709
BisI GCNGC 8 cut(s) 232, 378, 399, 498, 501, 504, 513, 786
BlsI GCNGC 8 cut(s) 233, 379, 400, 499, 502, 505, 514, 787
BmcAI AGTACT 1 cut(s) 637
BmeT110I CYCGRG 2 cut(s) 381, 402
BmiI GGNNCC 2 cut(s) 263, 418
BmsI GCATC 2 cut(s) 19, 784
BplI GAGNNNNNCTC 2 cut(s) 16, 48
Bpu10I CCTNAGC 1 cut(s) 218
BpuEI CTTGAG 2 cut(s) 18, 142
Bsa29I ATCGAT 1 cut(s) 117
BsaI GGTCTC 1 cut(s) 160
BsaJI CCNNGG 2 cut(s) 326, 483
Bsc4I CCNNNNNNNGG 3 cut(s) 213, 345, 445
Bse118I RCCGGY 1 cut(s) 466
Bse1I ACTGG 1 cut(s) 799
BseCI ATCGAT 1 cut(s) 117
BseDI CCNNGG 2 cut(s) 326, 483
BseGI GGATG 1 cut(s) 727
BseLI CCNNNNNNNGG 3 cut(s) 213, 345, 445
BseMII CTCAG 1 cut(s) 209
BseNI ACTGG 1 cut(s) 799
BseXI GCAGC 8 cut(s) 243, 385, 389, 484, 487, 490, 499, 797
BsgI GTGCAG 1 cut(s) 804
Bsh1236I CGCG 1 cut(s) 321
BshNI GGYRCC 2 cut(s) 261, 416
BshVI ATCGAT 1 cut(s) 117
BsiHKCI CYCGRG 2 cut(s) 381, 402
BsiSI CCGG 1 cut(s) 467
BslFI GGGAC 1 cut(s) 367
BslI CCNNNNNNNGG 3 cut(s) 213, 345, 445
BsmAI GTCTC 1 cut(s) 160
BsmFI GGGAC 1 cut(s) 367
BsmI GAATGC 1 cut(s) 291
Bso31I GGTCTC 1 cut(s) 160
BsoBI CYCGRG 2 cut(s) 381, 402
Bsp143I GATC 5 cut(s) 114, 136, 241, 709, 739
BspACI CCGC 1 cut(s) 319
BspCNI CTCAG 1 cut(s) 210
BspDI ATCGAT 1 cut(s) 117
BspFNI CGCG 1 cut(s) 321
BspLI GGNNCC 2 cut(s) 263, 418
BspT107I GGYRCC 2 cut(s) 261, 416
BspTNI GGTCTC 1 cut(s) 160
BsrFI RCCGGY 1 cut(s) 466
BsrI ACTGG 1 cut(s) 799
BssAI RCCGGY 1 cut(s) 466
BssECI CCNNGG 2 cut(s) 326, 483
BssMI GATC 5 cut(s) 114, 136, 241, 709, 739
BssT1I CCWWGG 1 cut(s) 483
Bst6I CTCTTC 4 cut(s) 61, 206, 567, 804
BstAPI GCANNNNNTGC 1 cut(s) 512
BstC8I GCNNGC 2 cut(s) 260, 323
BstDEI CTNAG 2 cut(s) 6, 218
BstF5I GGATG 1 cut(s) 727
BstFNI CGCG 1 cut(s) 321
BstHHI GCGC 2 cut(s) 291, 388
BstKTI GATC 5 cut(s) 117, 139, 244, 712, 742
BstMAI GTCTC 1 cut(s) 160
BstMBI GATC 5 cut(s) 114, 136, 241, 709, 739
BstMWI GCNNNNNNNGC 9 cut(s) 255, 297, 374, 383, 404, 503, 509, 512, 518
BstUI CGCG 1 cut(s) 321
BstV1I GCAGC 8 cut(s) 243, 385, 389, 484, 487, 490, 499, 797
BstX2I RGATCY 1 cut(s) 709
BstYI RGATCY 1 cut(s) 709
Bsu15I ATCGAT 1 cut(s) 117
BsuTUI ATCGAT 1 cut(s) 117
BtgZI GCGATG 1 cut(s) 264
BtsCI GGATG 1 cut(s) 727
BtsI GCAGTG 1 cut(s) 584
BtsIMutI CAGTG 2 cut(s) 237, 584
Cac8I GCNNGC 2 cut(s) 260, 323
CaiI CAGNNNCTG 1 cut(s) 794
CfoI GCGC 2 cut(s) 291, 388
Cfr10I RCCGGY 1 cut(s) 466
ClaI ATCGAT 1 cut(s) 117
Csp6I GTAC 3 cut(s) 281, 636, 648
CviAII CATG 4 cut(s) 104, 245, 718, 771
CviQI GTAC 3 cut(s) 281, 636, 648
DdeI CTNAG 2 cut(s) 6, 218
DpnI GATC 5 cut(s) 116, 138, 243, 711, 741
DpnII GATC 5 cut(s) 114, 136, 241, 709, 739
DraIII CACNNNGTG 1 cut(s) 394
Eam1104I CTCTTC 4 cut(s) 61, 206, 567, 804
EarI CTCTTC 4 cut(s) 61, 206, 567, 804
Eco130I CCWWGG 1 cut(s) 483
Eco31I GGTCTC 1 cut(s) 160
Eco88I CYCGRG 2 cut(s) 381, 402
EcoRI GAATTC 1 cut(s) 142
EcoT14I CCWWGG 1 cut(s) 483
ErhI CCWWGG 1 cut(s) 483
FaeI CATG 4 cut(s) 107, 248, 721, 774
FaiI YATR 7 cut(s) 20, 105, 246, 358, 684, 719, 772
FaqI GGGAC 1 cut(s) 367
FatI CATG 4 cut(s) 103, 244, 717, 770
FbaI TGATCA 3 cut(s) 136, 241, 739
Fnu4HI GCNGC 8 cut(s) 232, 378, 399, 498, 501, 504, 513, 786
FokI GGATG 1 cut(s) 734
Fsp4HI GCNGC 8 cut(s) 232, 378, 399, 498, 501, 504, 513, 786
FspBI CTAG 3 cut(s) 627, 639, 806
GlaI GCGC 2 cut(s) 290, 387
GluI GCNGC 8 cut(s) 232, 378, 399, 498, 501, 504, 513, 786
HapII CCGG 1 cut(s) 467
HhaI GCGC 2 cut(s) 291, 388
Hin1II CATG 4 cut(s) 107, 248, 721, 774
Hin6I GCGC 2 cut(s) 289, 386
HinP1I GCGC 2 cut(s) 289, 386
HinfI GANTC 3 cut(s) 107, 798, 812
HpaII CCGG 1 cut(s) 467
HphI GGTGA 1 cut(s) 544
Hpy188I TCNGA 4 cut(s) 141, 148, 313, 803
Hpy188III TCNNGA 2 cut(s) 339, 707
HpyAV CCTTC 1 cut(s) 713
HpyCH4V TGCA 2 cut(s) 506, 785
HpyF10VI GCNNNNNNNGC 9 cut(s) 255, 297, 374, 383, 404, 503, 509, 512, 518
HpyF3I CTNAG 2 cut(s) 6, 218
Hsp92II CATG 4 cut(s) 107, 248, 721, 774
HspAI GCGC 2 cut(s) 289, 386
Ksp22I TGATCA 3 cut(s) 136, 241, 739
Kzo9I GATC 5 cut(s) 114, 136, 241, 709, 739
LmnI GCTCC 1 cut(s) 228
LpnPI CCDG 6 cut(s) 244, 352, 453, 480, 720, 780
Lsp1109I GCAGC 8 cut(s) 243, 385, 389, 484, 487, 490, 499, 797
LweI GCATC 2 cut(s) 19, 784
MaeI CTAG 3 cut(s) 627, 639, 806
MalI GATC 5 cut(s) 116, 138, 243, 711, 741
MboI GATC 5 cut(s) 114, 136, 241, 709, 739
MflI RGATCY 1 cut(s) 709
MluCI AATT 6 cut(s) 142, 342, 433, 443, 692, 743
MlyI GAGTC 1 cut(s) 821
MseI TTAA 1 cut(s) 832
MslI CAYNNNNRTG 4 cut(s) 249, 769, 780, 820
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 1 cut(s) 467
Mva1269I GAATGC 1 cut(s) 291
MvnI CGCG 1 cut(s) 321
MwoI GCNNNNNNNGC 9 cut(s) 255, 297, 374, 383, 404, 503, 509, 512, 518
NdeII GATC 5 cut(s) 114, 136, 241, 709, 739
NlaIII CATG 4 cut(s) 107, 248, 721, 774
NlaIV GGNNCC 2 cut(s) 263, 418
NmeAIII GCCGAG 1 cut(s) 351
PcsI WCGNNNNNNNCGW 1 cut(s) 280
PctI GAATGC 1 cut(s) 291
PfeI GAWTC 2 cut(s) 107, 798
PflFI GACNNNGTC 1 cut(s) 152
PflMI CCANNNNNTGG 1 cut(s) 345
PkrI GCNGC 8 cut(s) 233, 379, 400, 499, 502, 505, 514, 787
PleI GAGTC 1 cut(s) 820
PpsI GAGTC 1 cut(s) 820
PspN4I GGNNCC 2 cut(s) 263, 418
PsrI GAACNNNNNNTAC 2 cut(s) 640, 672
PstNI CAGNNNCTG 1 cut(s) 794
PsuI RGATCY 1 cut(s) 709
PsyI GACNNNGTC 1 cut(s) 152
PvuII CAGCTG 1 cut(s) 368
RsaI GTAC 3 cut(s) 282, 637, 649
RsaNI GTAC 3 cut(s) 281, 636, 648
RseI CAYNNNNRTG 4 cut(s) 249, 769, 780, 820
SaqAI TTAA 1 cut(s) 832
SatI GCNGC 8 cut(s) 232, 378, 399, 498, 501, 504, 513, 786
Sau3AI GATC 5 cut(s) 114, 136, 241, 709, 739
ScaI AGTACT 1 cut(s) 637
SchI GAGTC 1 cut(s) 821
SfaNI GCATC 2 cut(s) 19, 784
SmiMI CAYNNNNRTG 4 cut(s) 249, 769, 780, 820
SmlI CTYRAG 2 cut(s) 33, 157
SmoI CTYRAG 2 cut(s) 33, 157
SpeI ACTAGT 2 cut(s) 626, 638
Sse9I AATT 6 cut(s) 142, 342, 433, 443, 692, 743
SsiI CCGC 1 cut(s) 319
SspMI CTAG 3 cut(s) 627, 639, 806
StyI CCWWGG 1 cut(s) 483
TaqI TCGA 1 cut(s) 117
TasI AATT 6 cut(s) 142, 342, 433, 443, 692, 743
TatI WGTACW 2 cut(s) 635, 647
TfiI GAWTC 2 cut(s) 107, 798
Tru1I TTAA 1 cut(s) 832
Tru9I TTAA 1 cut(s) 832
TscAI CASTG 2 cut(s) 244, 591
TseI GCWGC 8 cut(s) 231, 377, 398, 497, 500, 503, 512, 785
TspDTI ATGAA 4 cut(s) 615, 651, 706, 750
TspGWI ACGGA 1 cut(s) 569
TspRI CASTG 2 cut(s) 244, 591
Tth111I GACNNNGTC 1 cut(s) 152
Van91I CCANNNNNTGG 1 cut(s) 345
XapI RAATTY 3 cut(s) 142, 342, 433
XspI CTAG 3 cut(s) 627, 639, 806
ZrmI AGTACT 1 cut(s) 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.