FvH4_7g18510

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002588
Physical Location & Seq
Reverse (-)
15436321 .. 15438114
1794 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g18510.t1

Sequence Viewer

Length: 1794 bp
ATGGCGGGTTCCGGTCCTCGAGGATGGGCCGACACCAAAAGCCTGACCCTCCAGGTCCTCAACGGCCGGTGGTTCATGGTCTTTGCATGTCTCTTAATCATGTCCGCCGCCGGCGCCACCTACATGTTCGGCCTCTACTCTCCCGACATCAAGACAGCTCTAGCCTACGACCAATCTACACTCAACCTCCTCAGCTTCTTCAAAGATTTTGGATCCAACGTCGGCATCCTCTCCGGCCTCCTCAACGAGGTCACGCCGCCGTGGGTGGTCCTGCTGGTCGGCGCCGTCCTCAACTTCTTAGGATACCTAATGATCTGGCTCTCCGTCACCAAAAAAATCGCGAGACCAAAAGTCTGGCAGATGTGCTTGTACATTTGCATCGGCGCCAATTCTCAGTCGTTCGCCAACACTGGCTCGCTGGTCACCTGCGTCAAGAACTTCCCCGAGAGCCGCGGCGTCGTTTTAGGGCTTCTCAAGGGCTACGTCGGCCTCAGTGGCGCTATCATCACGCAGATGTACTTTGCTTTCTACTACAACGACACCAGGGCCTTGATTCTGCTCATCGGGTGGCTTCCGGCGGCGATCTCGTTCGCTTTTCTGAGGACTATTAGGATCATGAAGGTGATTAGACAAAGCAACGAGGTCCAGGTGTTCTACAACATGTTGTACATCTCTCTCGGCCTCGCCGGATTTCTGATGATCATGATCATAGTAGAGAATAGGGTGAACTTTACTCAACAAGAGTACGGCGCCAGCACGGCGATGGTGCTCTTCTTGCTCGCTCTTCCCGTTGTTGTTGTTGTAATCGAGGAGTACAAGCTTTGGGAGAGAAAACAGCTCGAGATGAATGGTGACGTCAAAATAGTGACGGAAAAGCCAGTCGTTCCCACGGAAGAACAATTAGAGGTGTCAAAGGAGCCGGTCACATATGAAACTCCTGAGTCCACAGAGGAACAACCTAGCCAATCTTGTTGGAAAACTTGTTTTAGGCCACCAAATAGAGGAGAAGACTACACTATTCTTCAAGCCCTATTCAGCATTGACATGCTCATACTATTCATGGCGTGCATATGTGGTGTTGGGGGCACATTGACAGCAATTGACAACTTGGGTCAGATTGGCACTTCGTTGGGATATCCCACACGCAGCAGGAAAACTTTCGTCGGCCTGGTCAGCATATGGAATTATCTCGGTAGAGTCGTCGCCGGATTCGTCTCGGAATTTTTACTAAAACGATACAAGTTCCCTCGTCCTCTCATTCTAACCCTAACCCTCTTTGTCTCTTGCTTTGGTCACCTCCTCATTGCCTTCAATGTTAAAAATGGTCTCTACCTAGCCTCGGTCATAATAGGGTTTTGCTTCGGCGCTCAATGGCCTTTGCTCTTCGCCATAATTTCTGAGCTCTTCGGGCTCAAGTACTACTCGACTTTGTACAATTTTGGTTCAGCCGCGAGTCCCGTCGGCTTGTACTTGCTCAACGTGAAACTCACCGGTCATGTATACGATAAGGAGGCGATGAAGCAATTGGTGGCCAAAGGGCTCGAGAGGAATAAAGACGAGGAGTTGACTTGTCTTGGTGGAGAGTGCTTCAAGTTGTCCTTCATTATAATCACAGCTGCCACATTTTTTGGGGCATTGGTTTCATTGATTTTAGTGATGAGGACAAGGAAGTTTTACCAGAGTGATATATACAAGAAGTTCCGAGAGGAGGCAGATAAAGCTGCTGAGACTGAAATGGCCATGGCACGAAATGGTGTCACCAATGGCCATAACCAAACCAATGGAAGATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 Nodulin-like

Protein Analysis

598

Amino Acids

66.58

Weight (kDa)

8.87

Isoelectric Point (pI)

37.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 16 - 168 5.1e-07 Major Facilitator Superfamily
Nodulin-like PF06813 23 - 268 1.1e-92 Nodulin-like
MFS_1 PF07690 262 - 485 8.3e-08 Major Facilitator Superfamily
NFD4_C PF23262 344 - 559 1.2e-17 NFD4, C-terminal domain
MFS_1 PF07690 373 - 546 8.2e-06 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1607
AarI CACCTGC 1 cut(s) 434
AatII GACGTC 1 cut(s) 858
AbsI CCTCGAGG 1 cut(s) 18
Acc36I ACCTGC 1 cut(s) 434
AccB1I GGYRCC 4 cut(s) 113, 281, 383, 749
AccI GTMKAC 1 cut(s) 1500
AccII CGCG 3 cut(s) 341, 453, 1451
AciI CCGC 8 cut(s) 5, 105, 108, 257, 451, 453, 578, 1449
AclWI GGATC 3 cut(s) 207, 220, 620
AcoI YGGCCR 4 cut(s) 64, 1530, 1737, 1765
AcsI RAATTY 1 cut(s) 1220
AcyI GRCGYC 6 cut(s) 114, 282, 384, 456, 750, 855
AfaI GTAC 8 cut(s) 371, 518, 668, 746, 815, 1418, 1433, 1469
AfiI CCNNNNNNNGG 4 cut(s) 247, 1001, 1339, 1708
AflIII ACRYGT 2 cut(s) 123, 660
AgeI ACCGGT 1 cut(s) 1490
AgsI TTSAA 4 cut(s) 202, 1025, 1312, 1591
AhdI GACNNNNNGTC 1 cut(s) 350
AjnI CCWGG 4 cut(s) 51, 542, 645, 1167
AluBI AGCT 7 cut(s) 158, 195, 820, 838, 1402, 1616, 1721
AluI AGCT 7 cut(s) 158, 195, 820, 838, 1402, 1616, 1721
Alw21I GWGCWC 2 cut(s) 771, 1404
Alw26I GTCTC 6 cut(s) 95, 337, 1219, 1285, 1331, 1721
AlwI GGATC 3 cut(s) 207, 220, 620
Ama87I CYCGRG 4 cut(s) 18, 443, 839, 1541
ApeKI GCWGC 3 cut(s) 1146, 1616, 1721
ApoI RAATTY 1 cut(s) 1220
AsiGI ACCGGT 1 cut(s) 1490
Asp700I GAANNNNTTC 1 cut(s) 1157
AspLEI GCGC 6 cut(s) 116, 284, 386, 500, 752, 1367
AspS9I GGNCC 6 cut(s) 14, 27, 55, 268, 546, 643
AsuHPI GGTGA 8 cut(s) 319, 415, 634, 736, 863, 1286, 1480, 1750
AvaI CYCGRG 4 cut(s) 18, 443, 839, 1541
AvaII GGWCC 4 cut(s) 14, 55, 268, 643
BaeGI GKGCMC 1 cut(s) 1088
BalI TGGCCA 3 cut(s) 1532, 1739, 1767
BamHI GGATCC 1 cut(s) 212
BanI GGYRCC 4 cut(s) 113, 281, 383, 749
BanII GRGCYC 3 cut(s) 1404, 1413, 1542
BbsI GAAGAC 1 cut(s) 1014
Bbv12I GWGCWC 2 cut(s) 771, 1404
BbvCI CCTCAGC 1 cut(s) 191
BbvI GCAGC 3 cut(s) 1158, 1603, 1708
BccI CCATC 2 cut(s) 18, 757
BceAI ACGGC 5 cut(s) 79, 244, 269, 763, 774
BciT130I CCWGG 4 cut(s) 53, 544, 647, 1169
BciVI GTATCC 1 cut(s) 296
BclI TGATCA 2 cut(s) 699, 705
BcoDI GTCTC 6 cut(s) 95, 337, 1219, 1285, 1331, 1721
BfaI CTAG 3 cut(s) 161, 960, 1334
BfoI RGCGCY 6 cut(s) 117, 285, 387, 501, 753, 1368
BfuAI ACCTGC 1 cut(s) 434
BfuI GTATCC 1 cut(s) 296
BglI GCCNNNNNGGC 2 cut(s) 495, 758
BisI GCNGC 9 cut(s) 108, 257, 451, 454, 579, 1147, 1449, 1617, 1722
BlsI GCNGC 9 cut(s) 109, 258, 452, 455, 580, 1148, 1450, 1618, 1723
BmcAI AGTACT 1 cut(s) 1418
Bme1390I CCNGG 4 cut(s) 53, 544, 647, 1169
Bme18I GGWCC 4 cut(s) 14, 55, 268, 643
BmeRI GACNNNNNGTC 1 cut(s) 350
BmeT110I CYCGRG 4 cut(s) 18, 443, 839, 1541
BmgT120I GGNCC 6 cut(s) 14, 27, 55, 268, 546, 643
BmiI GGNNCC 7 cut(s) 10, 115, 214, 283, 385, 751, 918
BmrFI CCNGG 4 cut(s) 53, 544, 647, 1169
BmsI GCATC 2 cut(s) 234, 387
BpiI GAAGAC 1 cut(s) 1014
BpmI CTGGAG 1 cut(s) 35
Bpu10I CCTNAGC 1 cut(s) 191
BpuEI CTTGAG 2 cut(s) 458, 1397
BsaBI GATNNNNATC 1 cut(s) 704
BsaHI GRCGYC 6 cut(s) 114, 282, 384, 456, 750, 855
BsaI GGTCTC 2 cut(s) 337, 1331
BsaJI CCNNGG 6 cut(s) 260, 451, 543, 888, 1338, 1740
BsaWI WCCGGW 2 cut(s) 11, 1490
BsaXI ACNNNNNCTCC 2 cut(s) 171, 201
Bsc4I CCNNNNNNNGG 4 cut(s) 247, 1001, 1339, 1708
Bse118I RCCGGY 4 cut(s) 66, 110, 919, 1490
Bse1I ACTGG 2 cut(s) 415, 878
Bse3DI GCAATG 1 cut(s) 1302
Bse8I GATNNNNATC 1 cut(s) 704
BseBI CCWGG 4 cut(s) 53, 544, 647, 1169
BseDI CCNNGG 6 cut(s) 260, 451, 543, 888, 1338, 1740
BseGI GGATG 2 cut(s) 29, 225
BseJI GATNNNNATC 1 cut(s) 704
BseLI CCNNNNNNNGG 4 cut(s) 247, 1001, 1339, 1708
BseMI GCAATG 1 cut(s) 1302
BseMII CTCAG 7 cut(s) 205, 407, 505, 590, 930, 1389, 1716
BseNI ACTGG 2 cut(s) 415, 878
BseRI GAGGAG 7 cut(s) 179, 230, 824, 1017, 1289, 1574, 1721
BseSI GKGCMC 1 cut(s) 1088
BseX3I CGGCCG 1 cut(s) 64
BseXI GCAGC 3 cut(s) 1158, 1603, 1708
Bsh1236I CGCG 3 cut(s) 341, 453, 1451
Bsh1285I CGRYCG 1 cut(s) 67
BshNI GGYRCC 4 cut(s) 113, 281, 383, 749
BshTI ACCGGT 1 cut(s) 1490
BsiEI CGRYCG 1 cut(s) 67
BsiHKAI GWGCWC 2 cut(s) 771, 1404
BsiHKCI CYCGRG 4 cut(s) 18, 443, 839, 1541
BsiSI CCGG 9 cut(s) 12, 67, 111, 234, 575, 687, 920, 1206, 1491
BslFI GGGAC 1 cut(s) 1440
BslI CCNNNNNNNGG 4 cut(s) 247, 1001, 1339, 1708
BsmAI GTCTC 6 cut(s) 95, 337, 1219, 1285, 1331, 1721
BsmBI CGTCTC 1 cut(s) 1219
BsmFI GGGAC 1 cut(s) 1440
Bso31I GGTCTC 2 cut(s) 337, 1331
BsoBI CYCGRG 4 cut(s) 18, 443, 839, 1541
Bsp1286I GDGCHC 5 cut(s) 771, 1088, 1404, 1413, 1542
Bsp1407I TGTACA 3 cut(s) 369, 666, 1431
Bsp143I GATC 7 cut(s) 212, 312, 582, 612, 699, 705, 1787
Bsp19I CCATGG 1 cut(s) 1740
Bsp68I TCGCGA 1 cut(s) 341
BspACI CCGC 8 cut(s) 5, 105, 108, 257, 451, 453, 578, 1449
BspCNI CTCAG 7 cut(s) 204, 406, 504, 591, 931, 1390, 1717
BspFNI CGCG 3 cut(s) 341, 453, 1451
BspHI TCATGA 2 cut(s) 615, 702
BspLI GGNNCC 7 cut(s) 10, 115, 214, 283, 385, 751, 918
BspMI ACCTGC 1 cut(s) 434
BspPI GGATC 3 cut(s) 207, 220, 620
BspQI GCTCTTC 4 cut(s) 776, 789, 1388, 1409
BspT107I GGYRCC 4 cut(s) 113, 281, 383, 749
BspTNI GGTCTC 2 cut(s) 337, 1331
BsrDI GCAATG 1 cut(s) 1302
BsrFI RCCGGY 4 cut(s) 66, 110, 919, 1490
BsrGI TGTACA 3 cut(s) 369, 666, 1431
BsrI ACTGG 2 cut(s) 415, 878
BssAI RCCGGY 4 cut(s) 66, 110, 919, 1490
BssECI CCNNGG 6 cut(s) 260, 451, 543, 888, 1338, 1740
BssMI GATC 7 cut(s) 212, 312, 582, 612, 699, 705, 1787
BssNAI GTATAC 1 cut(s) 1501
BssNI GRCGYC 6 cut(s) 114, 282, 384, 456, 750, 855
BssT1I CCWWGG 1 cut(s) 1740
Bst1107I GTATAC 1 cut(s) 1501
Bst2UI CCWGG 4 cut(s) 53, 544, 647, 1169
Bst6I CTCTTC 4 cut(s) 776, 789, 1388, 1409
BstACI GRCGYC 6 cut(s) 114, 282, 384, 456, 750, 855
BstAUI TGTACA 3 cut(s) 369, 666, 1431
BstC8I GCNNGC 5 cut(s) 112, 416, 754, 780, 1066
BstDEI CTNAG 8 cut(s) 191, 298, 393, 491, 599, 939, 1398, 1725
BstDSI CCRYGG 4 cut(s) 260, 451, 888, 1740
BstEII GGTNACC 2 cut(s) 421, 1292
BstENI CCTNNNNNAGG 1 cut(s) 245
BstF5I GGATG 2 cut(s) 29, 225
BstFNI CGCG 3 cut(s) 341, 453, 1451
BstH2I RGCGCY 6 cut(s) 117, 285, 387, 501, 753, 1368
BstHHI GCGC 6 cut(s) 116, 284, 386, 500, 752, 1367
BstKTI GATC 7 cut(s) 215, 315, 585, 615, 702, 708, 1790
BstMAI GTCTC 6 cut(s) 95, 337, 1219, 1285, 1331, 1721
BstMBI GATC 7 cut(s) 212, 312, 582, 612, 699, 705, 1787
BstMCI CGRYCG 1 cut(s) 67
BstMWI GCNNNNNNNGC 8 cut(s) 113, 486, 495, 758, 775, 1173, 1408, 1718
BstNI CCWGG 4 cut(s) 53, 544, 647, 1169
BstNSI RCATGY 4 cut(s) 90, 127, 664, 1048
BstPI GGTNACC 2 cut(s) 421, 1292
BstSCI CCNGG 4 cut(s) 51, 542, 645, 1167
BstSLI GKGCMC 1 cut(s) 1088
BstUI CGCG 3 cut(s) 341, 453, 1451
BstV1I GCAGC 3 cut(s) 1158, 1603, 1708
BstV2I GAAGAC 1 cut(s) 1014
BstX2I RGATCY 1 cut(s) 212
BstXI CCANNNNNNTGG 2 cut(s) 354, 1781
BstYI RGATCY 1 cut(s) 212
BstZ17I GTATAC 1 cut(s) 1501
BstZI CGGCCG 1 cut(s) 64
BsuI GTATCC 1 cut(s) 296
BtgI CCRYGG 4 cut(s) 260, 451, 888, 1740
BtgZI GCGATG 2 cut(s) 776, 1529
BtsCI GGATG 2 cut(s) 29, 225
BtsIMutI CAGTG 2 cut(s) 408, 499
BtuMI TCGCGA 1 cut(s) 341
BveI ACCTGC 1 cut(s) 434
Cac8I GCNNGC 5 cut(s) 112, 416, 754, 780, 1066
CciI TCATGA 2 cut(s) 615, 702
CfoI GCGC 6 cut(s) 116, 284, 386, 500, 752, 1367
Cfr10I RCCGGY 4 cut(s) 66, 110, 919, 1490
Cfr13I GGNCC 6 cut(s) 14, 27, 55, 268, 546, 643
Cfr42I CCGCGG 1 cut(s) 454
CseI GACGC 2 cut(s) 418, 445
Csp6I GTAC 8 cut(s) 370, 517, 667, 745, 814, 1417, 1432, 1468
CspAI ACCGGT 1 cut(s) 1490
CspCI CAANNNNNGTGG 2 cut(s) 1609, 1644
CviQI GTAC 8 cut(s) 370, 517, 667, 745, 814, 1417, 1432, 1468
DdeI CTNAG 8 cut(s) 191, 298, 393, 491, 599, 939, 1398, 1725
DinI GGCGCC 4 cut(s) 115, 283, 385, 751
DpnI GATC 7 cut(s) 214, 314, 584, 614, 701, 707, 1789
DpnII GATC 7 cut(s) 212, 312, 582, 612, 699, 705, 1787
DriI GACNNNNNGTC 1 cut(s) 350
EaeI YGGCCR 4 cut(s) 64, 1530, 1737, 1765
EagI CGGCCG 1 cut(s) 64
Eam1104I CTCTTC 4 cut(s) 776, 789, 1388, 1409
Eam1105I GACNNNNNGTC 1 cut(s) 350
EarI CTCTTC 4 cut(s) 776, 789, 1388, 1409
EciI GGCGGA 1 cut(s) 94
Ecl136II GAGCTC 1 cut(s) 1402
EclXI CGGCCG 1 cut(s) 64
Eco130I CCWWGG 1 cut(s) 1740
Eco24I GRGCYC 3 cut(s) 1404, 1413, 1542
Eco31I GGTCTC 2 cut(s) 337, 1331
Eco32I GATATC 1 cut(s) 1136
Eco47I GGWCC 4 cut(s) 14, 55, 268, 643
Eco52I CGGCCG 1 cut(s) 64
Eco53kI GAGCTC 1 cut(s) 1402
Eco88I CYCGRG 4 cut(s) 18, 443, 839, 1541
Eco91I GGTNACC 2 cut(s) 421, 1292
EcoICRI GAGCTC 1 cut(s) 1402
EcoNI CCTNNNNNAGG 1 cut(s) 245
EcoO109I RGGNCCY 2 cut(s) 55, 546
EcoO65I GGTNACC 2 cut(s) 421, 1292
EcoRII CCWGG 4 cut(s) 51, 542, 645, 1167
EcoRV GATATC 1 cut(s) 1136
EcoT14I CCWWGG 1 cut(s) 1740
EcoT38I GRGCYC 3 cut(s) 1404, 1413, 1542
EgeI GGCGCC 4 cut(s) 115, 283, 385, 751
EheI GGCGCC 4 cut(s) 115, 283, 385, 751
ErhI CCWWGG 1 cut(s) 1740
Esp3I CGTCTC 1 cut(s) 1219
FalI AAGNNNNNCTT 2 cut(s) 1583, 1615
FaqI GGGAC 1 cut(s) 1440
FauNDI CATATG 3 cut(s) 928, 1070, 1178
FbaI TGATCA 2 cut(s) 699, 705
FblI GTMKAC 1 cut(s) 1500
Fnu4HI GCNGC 9 cut(s) 108, 257, 451, 454, 579, 1147, 1449, 1617, 1722
FokI GGATG 2 cut(s) 36, 212
FriOI GRGCYC 3 cut(s) 1404, 1413, 1542
Fsp4HI GCNGC 9 cut(s) 108, 257, 451, 454, 579, 1147, 1449, 1617, 1722
FspBI CTAG 3 cut(s) 161, 960, 1334
GlaI GCGC 6 cut(s) 115, 283, 385, 499, 751, 1366
GluI GCNGC 9 cut(s) 108, 257, 451, 454, 579, 1147, 1449, 1617, 1722
GsuI CTGGAG 1 cut(s) 35
HaeII RGCGCY 6 cut(s) 117, 285, 387, 501, 753, 1368
HapII CCGG 9 cut(s) 12, 67, 111, 234, 575, 687, 920, 1206, 1491
HgaI GACGC 2 cut(s) 418, 445
HhaI GCGC 6 cut(s) 116, 284, 386, 500, 752, 1367
Hin1I GRCGYC 6 cut(s) 114, 282, 384, 456, 750, 855
Hin6I GCGC 6 cut(s) 114, 282, 384, 498, 750, 1365
HinP1I GCGC 6 cut(s) 114, 282, 384, 498, 750, 1365
HincII GTYRAC 1 cut(s) 1566
HindII GTYRAC 1 cut(s) 1566
HindIII AAGCTT 1 cut(s) 818
HinfI GANTC 5 cut(s) 553, 941, 1197, 1209, 1453
HpaII CCGG 9 cut(s) 12, 67, 111, 234, 575, 687, 920, 1206, 1491
HphI GGTGA 8 cut(s) 319, 415, 634, 736, 863, 1286, 1480, 1750
Hpy166II GTNNAC 4 cut(s) 727, 945, 1501, 1566
Hpy188I TCNGA 6 cut(s) 600, 696, 1116, 1219, 1399, 1703
Hpy188III TCNNGA 9 cut(s) 143, 151, 340, 433, 616, 703, 841, 938, 1543
Hpy8I GTNNAC 4 cut(s) 727, 945, 1501, 1566
Hpy99I CGWCG 6 cut(s) 224, 461, 488, 1166, 1205, 1463
HpyAV CCTTC 3 cut(s) 613, 1318, 1609
HpyCH4IV ACGT 4 cut(s) 219, 483, 855, 1479
HpyCH4V TGCA 3 cut(s) 86, 378, 1068
HpyF10VI GCNNNNNNNGC 8 cut(s) 113, 486, 495, 758, 775, 1173, 1408, 1718
HpyF3I CTNAG 8 cut(s) 191, 298, 393, 491, 599, 939, 1398, 1725
HpySE526I ACGT 4 cut(s) 219, 483, 855, 1479
Hsp92I GRCGYC 6 cut(s) 114, 282, 384, 456, 750, 855
HspAI GCGC 6 cut(s) 114, 282, 384, 498, 750, 1365
KasI GGCGCC 4 cut(s) 113, 281, 383, 749
KroI GCCGGC 1 cut(s) 110
KroNI GCCGGC 1 cut(s) 112
Ksp22I TGATCA 2 cut(s) 699, 705
KspI CCGCGG 1 cut(s) 454
Kzo9I GATC 7 cut(s) 212, 312, 582, 612, 699, 705, 1787
LguI GCTCTTC 4 cut(s) 776, 789, 1388, 1409
LmnI GCTCC 1 cut(s) 916
Lsp1109I GCAGC 3 cut(s) 1158, 1603, 1708
LweI GCATC 2 cut(s) 234, 387
MaeI CTAG 3 cut(s) 161, 960, 1334
MaeII ACGT 4 cut(s) 219, 483, 855, 1479
MaeIII GTNAC 8 cut(s) 250, 325, 421, 851, 865, 922, 1292, 1756
MalI GATC 7 cut(s) 214, 314, 584, 614, 701, 707, 1789
MboI GATC 7 cut(s) 212, 312, 582, 612, 699, 705, 1787
MboII GAAGA 8 cut(s) 190, 763, 776, 905, 1013, 1019, 1375, 1396
MfeI CAATTG 2 cut(s) 1098, 1523
MflI RGATCY 1 cut(s) 212
MhlI GDGCHC 5 cut(s) 771, 1088, 1404, 1413, 1542
MlsI TGGCCA 3 cut(s) 1532, 1739, 1767
MluCI AATT 8 cut(s) 388, 899, 1098, 1183, 1220, 1392, 1435, 1523
MluNI TGGCCA 3 cut(s) 1532, 1739, 1767
Mly113I GGCGCC 4 cut(s) 114, 282, 384, 750
MlyI GAGTC 3 cut(s) 950, 1206, 1462
MmeI TCCRAC 2 cut(s) 240, 953
Mox20I TGGCCA 3 cut(s) 1532, 1739, 1767
MreI CGCCGGCG 1 cut(s) 110
MroNI GCCGGC 1 cut(s) 110
MroXI GAANNNNTTC 1 cut(s) 1157
MscI TGGCCA 3 cut(s) 1532, 1739, 1767
MseI TTAA 2 cut(s) 95, 1317
MslI CAYNNNNRTG 5 cut(s) 122, 512, 620, 761, 1043
Msp20I TGGCCA 3 cut(s) 1532, 1739, 1767
MspA1I CMGCKG 2 cut(s) 453, 1616
MspI CCGG 9 cut(s) 12, 67, 111, 234, 575, 687, 920, 1206, 1491
MspR9I CCNGG 4 cut(s) 53, 544, 647, 1169
MunI CAATTG 2 cut(s) 1098, 1523
MvaI CCWGG 4 cut(s) 53, 544, 647, 1169
MvnI CGCG 3 cut(s) 341, 453, 1451
MwoI GCNNNNNNNGC 8 cut(s) 113, 486, 495, 758, 775, 1173, 1408, 1718
NaeI GCCGGC 1 cut(s) 112
NarI GGCGCC 4 cut(s) 114, 282, 384, 750
NcoI CCATGG 1 cut(s) 1740
NdeI CATATG 3 cut(s) 928, 1070, 1178
NdeII GATC 7 cut(s) 212, 312, 582, 612, 699, 705, 1787
NgoMIV GCCGGC 1 cut(s) 110
NlaIV GGNNCC 7 cut(s) 10, 115, 214, 283, 385, 751, 918
NmeAIII GCCGAG 1 cut(s) 657
NmuCI GTSAC 8 cut(s) 250, 325, 421, 851, 865, 922, 1292, 1756
NruI TCGCGA 1 cut(s) 341
NspI RCATGY 4 cut(s) 90, 127, 664, 1048
PaeR7I CTCGAG 3 cut(s) 18, 839, 1541
PagI TCATGA 2 cut(s) 615, 702
PaqCI CACCTGC 1 cut(s) 434
PciI ACATGT 2 cut(s) 123, 660
PciSI GCTCTTC 4 cut(s) 776, 789, 1388, 1409
PcsI WCGNNNNNNNCGW 3 cut(s) 786, 1197, 1209
PdiI GCCGGC 1 cut(s) 112
PdmI GAANNNNTTC 1 cut(s) 1157
PfeI GAWTC 2 cut(s) 553, 1209
PinAI ACCGGT 1 cut(s) 1490
PkrI GCNGC 9 cut(s) 109, 258, 452, 455, 580, 1148, 1450, 1618, 1723
PleI GAGTC 3 cut(s) 949, 1205, 1461
PluTI GGCGCC 4 cut(s) 117, 285, 387, 753
PpsI GAGTC 3 cut(s) 949, 1205, 1461
PpuMI RGGWCCY 1 cut(s) 55
PscI ACATGT 2 cut(s) 123, 660
PsiI TTATAA 1 cut(s) 1607
Psp124BI GAGCTC 1 cut(s) 1404
Psp5II RGGWCCY 1 cut(s) 55
Psp6I CCWGG 4 cut(s) 51, 542, 645, 1167
PspEI GGTNACC 2 cut(s) 421, 1292
PspGI CCWGG 4 cut(s) 51, 542, 645, 1167
PspN4I GGNNCC 7 cut(s) 10, 115, 214, 283, 385, 751, 918
PspPI GGNCC 6 cut(s) 14, 27, 55, 268, 546, 643
PspPPI RGGWCCY 1 cut(s) 55
PspXI VCTCGAGB 1 cut(s) 18
PsuI RGATCY 1 cut(s) 212
PvuII CAGCTG 1 cut(s) 1616
RruI TCGCGA 1 cut(s) 341
RsaI GTAC 8 cut(s) 371, 518, 668, 746, 815, 1418, 1433, 1469
RsaNI GTAC 8 cut(s) 370, 517, 667, 745, 814, 1417, 1432, 1468
RseI CAYNNNNRTG 5 cut(s) 122, 512, 620, 761, 1043
SacI GAGCTC 1 cut(s) 1404
SacII CCGCGG 1 cut(s) 454
SapI GCTCTTC 4 cut(s) 776, 789, 1388, 1409
SaqAI TTAA 2 cut(s) 95, 1317
SatI GCNGC 9 cut(s) 108, 257, 451, 454, 579, 1147, 1449, 1617, 1722
Sau3AI GATC 7 cut(s) 212, 312, 582, 612, 699, 705, 1787
Sau96I GGNCC 6 cut(s) 14, 27, 55, 268, 546, 643
ScaI AGTACT 1 cut(s) 1418
SchI GAGTC 3 cut(s) 950, 1206, 1462
ScrFI CCNGG 4 cut(s) 53, 544, 647, 1169
SduI GDGCHC 5 cut(s) 771, 1088, 1404, 1413, 1542
SfaNI GCATC 2 cut(s) 234, 387
SfoI GGCGCC 4 cut(s) 115, 283, 385, 751
Sfr274I CTCGAG 3 cut(s) 18, 839, 1541
Sfr303I CCGCGG 1 cut(s) 454
SgrAI CRCCGGYG 1 cut(s) 110
SgrBI CCGCGG 1 cut(s) 454
SinI GGWCC 4 cut(s) 14, 55, 268, 643
SlaI CTCGAG 3 cut(s) 18, 839, 1541
SmiMI CAYNNNNRTG 5 cut(s) 122, 512, 620, 761, 1043
SmlI CTYRAG 5 cut(s) 18, 473, 839, 1412, 1541
SmoI CTYRAG 5 cut(s) 18, 473, 839, 1412, 1541
Sse9I AATT 8 cut(s) 388, 899, 1098, 1183, 1220, 1392, 1435, 1523
SsiI CCGC 8 cut(s) 5, 105, 108, 257, 451, 453, 578, 1449
SspDI GGCGCC 4 cut(s) 113, 281, 383, 749
SspMI CTAG 3 cut(s) 161, 960, 1334
SstI GAGCTC 1 cut(s) 1404
StyD4I CCNGG 4 cut(s) 51, 542, 645, 1167
StyI CCWWGG 1 cut(s) 1740
TaiI ACGT 4 cut(s) 222, 486, 858, 1482
TaqI TCGA 5 cut(s) 19, 807, 840, 1424, 1542
TaqII GACCGA 1 cut(s) 1330
TasI AATT 8 cut(s) 388, 899, 1098, 1183, 1220, 1392, 1435, 1523
TatI WGTACW 7 cut(s) 369, 516, 666, 813, 1416, 1431, 1467
TauI GCSGC 6 cut(s) 110, 259, 453, 456, 581, 1451
TfiI GAWTC 2 cut(s) 553, 1209
Tru1I TTAA 2 cut(s) 95, 1317
Tru9I TTAA 2 cut(s) 95, 1317
TscAI CASTG 2 cut(s) 415, 499
TseFI GTSAC 8 cut(s) 250, 325, 421, 851, 865, 922, 1292, 1756
TseI GCWGC 3 cut(s) 1146, 1616, 1721
Tsp45I GTSAC 8 cut(s) 250, 325, 421, 851, 865, 922, 1292, 1756
TspDTI ATGAA 8 cut(s) 64, 632, 860, 945, 1048, 1532, 1591, 1632
TspGWI ACGGA 3 cut(s) 313, 884, 905
TspRI CASTG 2 cut(s) 415, 499
VpaK11BI GGWCC 4 cut(s) 14, 55, 268, 643
XagI CCTNNNNNAGG 1 cut(s) 245
XapI RAATTY 1 cut(s) 1220
XceI RCATGY 4 cut(s) 90, 127, 664, 1048
XcmI CCANNNNNNNNNTGG 1 cut(s) 760
XhoI CTCGAG 3 cut(s) 18, 839, 1541
XmiI GTMKAC 1 cut(s) 1500
XmnI GAANNNNTTC 1 cut(s) 1157
XspI CTAG 3 cut(s) 161, 960, 1334
ZraI GACGTC 1 cut(s) 856
ZrmI AGTACT 1 cut(s) 1418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.