Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0002588

Physical Location & Seq

Genomic Coordinates Reverse (-)

12986272 .. 12988110

Gene Structure 1839 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g10870.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1839 bp

ATGGTGGCGGCTGAGTTCACCTCGGGTTTTGGGAACAAGGAGATGAGGAGCTTTGGCTTTCAAGTTCTCCATGGCAGATGGTTCATGGTTTTTGCATCAATGCTTATCTTATCCATGTCCGGCGCGACGTACATGTTCGGCCTCTACTCAAATGAGGTCAAAACCTCTTTAGGCTATGATCAAACGACAGTCAATCTTCTAAGCTTCTTCAAGGACTTGGGAGGTAATGTCGGGATCATAGCCGGGCTCATCAACGAGGTAACAGGGCCATGGGTGGTGCTGTTAATCGGGGCGCTGATGAACTTTTTCGGGTACTTCATGATATGGCTGGCGGTGACTGGTCGGACGTCGAAGCCTCGCATGTGGCAGATGTGTCTGTACATCTGCATAGGTGCAAATTCACAGACGTTTGCAAACACCGGCGCGGTGGTGAGCTGTGTGAAGAATTTCCCGGAAAGTAGAGGCAGTGTTCTGGGGATTTTGAAGGGACTTGTTGGCCTGAGTGGTGCAATTATCACGCAGCTCTTCCATGCTTTCTATGGAAACGACGTAAAGTCTCTGATTTTGTTAATTGGTTGGCTTCCGGCTTCTGTTTCGACGGTGTTTCTTCCGATTATTCGGTTGATAAAGTACGTCAGACAATCGAATGAGCTCAAGGTTTTCTACAATCTTCTCTACATTACTCTCGGCCTAGCTGGATTTCTCATGGCTATAATTATTATACAGCACAAGCTCAAATTTTCAAGAATTGAGTACATCGCAAGTGCTTCATTTGTGACTCTGTTACTCTGTCTACCCCTCAGCGTTGTTATATTCGAAGAATTGAAGATCCGAAAGAGTAAAATAAGAGCGTTGAACGATCAATCTGAGCTCAAAGTAGTAGCTGAGAATCCAATTCCAGCTCCTCCTCCTCCTCCTCCTGTTGAAGTAGCACCGCAACTTGCTGCAGAGTCTGCACAAACACAGAGCCAGCAGAAGCCAACTTCTTGCTGCTTCAGCCGGCCGAATAGAGGTGAGGACTACACCATACTGCAAGCAGTTTTCAGCATCGACATGCTGATCTTGTTCCTTGCCACAAGCTGTGGTGTCGGTGGGACTTTAACCGCCATCGACAACTTGGGTCAGATTGGGAGCTCATTAGGTTACCCATCTGACAGCATAAGCACTTTTATATCTCTGGTGAGCATATGGAATTACCTCGGAAGAGTCACTTCTGGCTTCTCCTCGGAAGTGCTTTTGAAAAAATACATGTTCCCTCGGCCCCTCATGCTCACTCTTGTGCTTCTCTTGTCATGTGTTGGCCATCTTCTCATAGCCTTTGGCGTCCCCAATTCTCTTTACTTTGCTTCGGTAATCATTGGGTTCTGTTTTGGGGCACAATGGCCATTGATTTTCGCCATCGTATCGGAGATCTTTGGCCTCAAGTACTACGCTACTCTGATCAATGTTAGTGGTGCTGCTAGCCCTATTGGGGCTTATCTTCTCAATGTCAAAGTGGCCGGAAATTTATACGATGCGGAGGCCTTGAAGCAATTGAAGGCTTTGGGAAGGATTAGAAAAGCAGGGGAGGACTTACGTTGTGTGGGAGTGCAATGCTACAAATTGGCTTTCATTATAATGGCTGCTGTGGCATTTTTTGGGAGCCTTGTTTCTTTTATTTTGGTAATTAGGACAAGAAAATTTTACAAAAGTGATATTTACAAGAAGTTCAGAGAGGCAGCGGAGGCAACTGAATCGGATAATGTAGCTGCTGCTTCAGGTGGTTTGGTGCCTCCGAGAGAAATGGAGTCCAAGGCTCCAGTGAATTCTGCAAATCCATCAACCTCCTCAGATGGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MFS_1 Nodulin-like

Protein Analysis

613

Amino Acids

66.65

Weight (kDa)

9.16

Isoelectric Point (pI)

34.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MFS_1	PF07690	21 - 171	1e-06	Major Facilitator Superfamily
Nodulin-like	PF06813	26 - 271	1.9e-86	Nodulin-like
NFD4_C	PF23262	351 - 562	7.4e-17	NFD4, C-terminal domain
MFS_1	PF07690	363 - 559	1.6e-06	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002588)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39210
fragaria_vesca	FvH4_6g16630 FvH4_7g18510
malus_domestica	MD01G1101400.v1.1 MD04G1090400.v1.1 MD07G1168000.v1.1
prunus_persica	Prupe.2G206600_v2.0.a1 Prupe.6G220200_v2.0.a1 Prupe.7G127100_v2.0.a1
pyrus_communis	pycom07g16250 pycom12g10870
rosa_chinensis	RchiOBHm_Chr1g0334871 RchiOBHm_Chr1g0362291 RchiOBHm_Chr3g0470591
rosa_laevigata	RLG00000024257 RLG00000027655 RLG00000029413
rosa_multiflora	Rmu_co8175752.1_g000001 Rmu_sc0000574.1_g000047 Rmu_sc0002115.1_g000014 Rmu_sc0007125.1_g000010 Rmu_ssc0000009.1_g000029
rosa_roxburghii	Rroxscaffold_4G00294280 Rroxscaffold_6G00410710
rosa_rugosa	Rorug01G0117400 Rorug01G0297200 Rorug03G0110100.1
rosa_samantha	Rh1AG306000 Rh1BG108600 Rh1BG269500 Rh1CG287000 Rh1DG145500 Rh1DG299600 Rh3AG160300 Rh3BG184800 Rh3CG175300 Rh3DG189900
rosa_wichuraiana	Rw1G011680 Rw1G027150 Rw3G015160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1616
AatII	GACGTC	1 cut(s)	350
AccB1I	GGYRCC	1 cut(s)	1768
AccI	GTMKAC	1 cut(s)	793
AccII	CGCG	2 cut(s)	125, 425
AciI	CCGC	7 cut(s)	8, 332, 425, 935, 1104, 1517, 1721
AclWI	GGATC	2 cut(s)	242, 823
AcoI	YGGCCR	4 cut(s)	1001, 1300, 1382, 1497
AcsI	RAATTY	6 cut(s)	397, 445, 737, 1504, 1679, 1804
AcuI	CTGAAG	2 cut(s)	979, 1740
AcyI	GRCGYC	2 cut(s)	347, 1323
AfaI	GTAC	6 cut(s)	131, 314, 380, 632, 755, 1427
AfiI	CCNNNNNNNGG	2 cut(s)	1010, 1471
AflIII	ACRYGT	2 cut(s)	132, 1248
AhdI	GACNNNNNGTC	1 cut(s)	553
AleI	CACNNNNGTG	1 cut(s)	1277
Alw21I	GWGCWC	3 cut(s)	654, 873, 1136
Alw26I	GTCTC	1 cut(s)	561
AlwI	GGATC	2 cut(s)	242, 823
AlwNI	CAGNNNCTG	1 cut(s)	953
Ama87I	CYCGRG	1 cut(s)	22
ApeKI	GCWGC	8 cut(s)	520, 944, 990, 1457, 1622, 1718, 1748, 1751
ApoI	RAATTY	6 cut(s)	397, 445, 737, 1504, 1679, 1804
Asp700I	GAANNNNTTC	2 cut(s)	305, 446
AspLEI	GCGC	3 cut(s)	125, 295, 425
AspS9I	GGNCC	2 cut(s)	266, 1260
AsuC2I	CCSGG	2 cut(s)	244, 452
AsuHPI	GGTGA	5 cut(s)	10, 346, 442, 1025, 1192
AsuII	TTCGAA	1 cut(s)	816
AsuNHI	GCTAGC	1 cut(s)	1460
AvaI	CYCGRG	1 cut(s)	22
BaeGI	GKGCMC	1 cut(s)	1378
BalI	TGGCCA	2 cut(s)	1302, 1384
BanI	GGYRCC	1 cut(s)	1768
BanII	GRGCYC	4 cut(s)	249, 654, 873, 1136
Bbv12I	GWGCWC	3 cut(s)	654, 873, 1136
BbvCI	CCTCAGC	1 cut(s)	800
BbvI	GCAGC	8 cut(s)	532, 931, 977, 1444, 1609, 1730, 1735, 1738
BccI	CCATC	7 cut(s)	72, 1115, 1156, 1311, 1406, 1825, 1826
BcgI	CGANNNNNNTGC	2 cut(s)	1716, 1750
BclI	TGATCA	2 cut(s)	178, 1440
BcnI	CCSGG	2 cut(s)	244, 452
BcoDI	GTCTC	1 cut(s)	561
BfaI	CTAG	2 cut(s)	692, 1461
BfmI	CTRYAG	1 cut(s)	945
BfoI	RGCGCY	1 cut(s)	296
BglII	AGATCT	1 cut(s)	1410
BisI	GCNGC	9 cut(s)	9, 521, 945, 991, 1458, 1623, 1719, 1749, 1752
BlsI	GCNGC	9 cut(s)	10, 522, 946, 992, 1459, 1624, 1720, 1750, 1753
BmcAI	AGTACT	1 cut(s)	1427
Bme1390I	CCNGG	2 cut(s)	244, 452
BmeRI	GACNNNNNGTC	1 cut(s)	553
BmeT110I	CYCGRG	1 cut(s)	22
BmgT120I	GGNCC	2 cut(s)	266, 1260
BmiI	GGNNCC	4 cut(s)	1262, 1643, 1770, 1797
BmrFI	CCNGG	2 cut(s)	244, 452
BmsI	GCATC	3 cut(s)	104, 1056, 1504
BmtI	GCTAGC	1 cut(s)	1464
BplI	GAGNNNNNCTC	1 cut(s)	37
BpmI	CTGGAG	1 cut(s)	1782
Bpu10I	CCTNAGC	1 cut(s)	800
Bpu14I	TTCGAA	1 cut(s)	816
BpuEI	CTTGAG	2 cut(s)	638, 1406
BpuMI	CCSGG	2 cut(s)	244, 452
BsaHI	GRCGYC	2 cut(s)	347, 1323
BsaJI	CCNNGG	7 cut(s)	21, 70, 269, 1198, 1224, 1256, 1791
BsaXI	ACNNNNNCTCC	2 cut(s)	213, 243
Bsc4I	CCNNNNNNNGG	2 cut(s)	1010, 1471
Bse118I	RCCGGY	2 cut(s)	419, 999
Bse1I	ACTGG	2 cut(s)	343, 1799
Bse3DI	GCAATG	1 cut(s)	1598
BseDI	CCNNGG	7 cut(s)	21, 70, 269, 1198, 1224, 1256, 1791
BseLI	CCNNNNNNNGG	2 cut(s)	1010, 1471
BseMI	GCAATG	1 cut(s)	1598
BseMII	CTCAG	4 cut(s)	491, 814, 858, 876
BseNI	ACTGG	2 cut(s)	343, 1799
BseRI	GAGGAG	8 cut(s)	61, 894, 897, 900, 903, 906, 1213, 1816
BseSI	GKGCMC	1 cut(s)	1378
BseX3I	CGGCCG	1 cut(s)	1001
BseXI	GCAGC	8 cut(s)	532, 931, 977, 1444, 1609, 1730, 1735, 1738
BsgI	GTGCAG	1 cut(s)	939
Bsh1236I	CGCG	2 cut(s)	125, 425
Bsh1285I	CGRYCG	1 cut(s)	1004
BshNI	GGYRCC	1 cut(s)	1768
BsiEI	CGRYCG	1 cut(s)	1004
BsiHKAI	GWGCWC	3 cut(s)	654, 873, 1136
BsiHKCI	CYCGRG	1 cut(s)	22
BsiSI	CCGG	7 cut(s)	120, 243, 420, 452, 584, 1000, 1500
BslFI	GGGAC	3 cut(s)	501, 1108, 1310
BslI	CCNNNNNNNGG	2 cut(s)	1010, 1471
BsmAI	GTCTC	1 cut(s)	561
BsmFI	GGGAC	3 cut(s)	501, 1108, 1310
BsoBI	CYCGRG	1 cut(s)	22
Bsp119I	TTCGAA	1 cut(s)	816
Bsp1286I	GDGCHC	5 cut(s)	249, 654, 873, 1136, 1378
Bsp1407I	TGTACA	1 cut(s)	378
Bsp143I	GATC	7 cut(s)	178, 234, 828, 859, 1059, 1410, 1440
Bsp19I	CCATGG	2 cut(s)	70, 269
BspACI	CCGC	7 cut(s)	8, 332, 425, 935, 1104, 1517, 1721
BspCNI	CTCAG	5 cut(s)	4, 492, 813, 859, 877
BspFNI	CGCG	2 cut(s)	125, 425
BspHI	TCATGA	1 cut(s)	318
BspLI	GGNNCC	4 cut(s)	1262, 1643, 1770, 1797
BspMAI	CTGCAG	1 cut(s)	949
BspOI	GCTAGC	1 cut(s)	1464
BspPI	GGATC	2 cut(s)	242, 823
BspQI	GCTCTTC	1 cut(s)	530
BspT104I	TTCGAA	1 cut(s)	816
BspT107I	GGYRCC	1 cut(s)	1768
BsrDI	GCAATG	1 cut(s)	1598
BsrFI	RCCGGY	2 cut(s)	419, 999
BsrGI	TGTACA	1 cut(s)	378
BsrI	ACTGG	2 cut(s)	343, 1799
BssAI	RCCGGY	2 cut(s)	419, 999
BssECI	CCNNGG	7 cut(s)	21, 70, 269, 1198, 1224, 1256, 1791
BssMI	GATC	7 cut(s)	178, 234, 828, 859, 1059, 1410, 1440
BssNI	GRCGYC	2 cut(s)	347, 1323
BssT1I	CCWWGG	3 cut(s)	70, 269, 1791
Bst4CI	ACNGT	2 cut(s)	190, 601
Bst6I	CTCTTC	2 cut(s)	530, 1198
BstACI	GRCGYC	2 cut(s)	347, 1323
BstAPI	GCANNNNNTGC	1 cut(s)	953
BstAUI	TGTACA	1 cut(s)	378
BstBI	TTCGAA	1 cut(s)	816
BstC8I	GCNNGC	5 cut(s)	330, 971, 1001, 1035, 1462
BstDEI	CTNAG	7 cut(s)	12, 200, 500, 800, 867, 885, 1828
BstDSI	CCRYGG	2 cut(s)	70, 269
BstEII	GGTNACC	1 cut(s)	1142
BstFNI	CGCG	2 cut(s)	125, 425
BstH2I	RGCGCY	1 cut(s)	296
BstHHI	GCGC	3 cut(s)	125, 295, 425
BstKTI	GATC	7 cut(s)	181, 237, 831, 862, 1062, 1413, 1443
BstMAI	GTCTC	1 cut(s)	561
BstMBI	GATC	7 cut(s)	178, 234, 828, 859, 1059, 1410, 1440
BstMCI	CGRYCG	1 cut(s)	1004
BstMWI	GCNNNNNNNGC	5 cut(s)	953, 996, 1267, 1628, 1724
BstNSI	RCATGY	4 cut(s)	136, 364, 1057, 1252
BstPI	GGTNACC	1 cut(s)	1142
BstSCI	CCNGG	2 cut(s)	242, 450
BstSFI	CTRYAG	1 cut(s)	945
BstSLI	GKGCMC	1 cut(s)	1378
BstUI	CGCG	2 cut(s)	125, 425
BstV1I	GCAGC	8 cut(s)	532, 931, 977, 1444, 1609, 1730, 1735, 1738
BstX2I	RGATCY	2 cut(s)	828, 1410
BstYI	RGATCY	2 cut(s)	828, 1410
BstZI	CGGCCG	1 cut(s)	1001
BtgI	CCRYGG	2 cut(s)	70, 269
BtgZI	GCGATG	1 cut(s)	742
BtsI	GCAGTG	1 cut(s)	472
BtsIMutI	CAGTG	2 cut(s)	472, 1806
Cac8I	GCNNGC	5 cut(s)	330, 971, 1001, 1035, 1462
CaiI	CAGNNNCTG	1 cut(s)	953
CciI	TCATGA	1 cut(s)	318
CfoI	GCGC	3 cut(s)	125, 295, 425
Cfr10I	RCCGGY	2 cut(s)	419, 999
Cfr13I	GGNCC	2 cut(s)	266, 1260
CseI	GACGC	1 cut(s)	1312
Csp6I	GTAC	6 cut(s)	130, 313, 379, 631, 754, 1426
CspCI	CAANNNNNGTGG	2 cut(s)	1432, 1467
CviQI	GTAC	6 cut(s)	130, 313, 379, 631, 754, 1426
DdeI	CTNAG	7 cut(s)	12, 200, 500, 800, 867, 885, 1828
DpnI	GATC	7 cut(s)	180, 236, 830, 861, 1061, 1412, 1442
DpnII	GATC	7 cut(s)	178, 234, 828, 859, 1059, 1410, 1440
DriI	GACNNNNNGTC	1 cut(s)	553
EaeI	YGGCCR	4 cut(s)	1001, 1300, 1382, 1497
EagI	CGGCCG	1 cut(s)	1001
Eam1104I	CTCTTC	2 cut(s)	530, 1198
Eam1105I	GACNNNNNGTC	1 cut(s)	553
EarI	CTCTTC	2 cut(s)	530, 1198
Ecl136II	GAGCTC	3 cut(s)	652, 871, 1134
EclXI	CGGCCG	1 cut(s)	1001
Eco130I	CCWWGG	3 cut(s)	70, 269, 1791
Eco147I	AGGCCT	1 cut(s)	1523
Eco24I	GRGCYC	4 cut(s)	249, 654, 873, 1136
Eco52I	CGGCCG	1 cut(s)	1001
Eco53kI	GAGCTC	3 cut(s)	652, 871, 1134
Eco57I	CTGAAG	2 cut(s)	979, 1740
Eco88I	CYCGRG	1 cut(s)	22
Eco91I	GGTNACC	1 cut(s)	1142
EcoICRI	GAGCTC	3 cut(s)	652, 871, 1134
EcoO65I	GGTNACC	1 cut(s)	1142
EcoRI	GAATTC	1 cut(s)	1804
EcoT14I	CCWWGG	3 cut(s)	70, 269, 1791
EcoT38I	GRGCYC	4 cut(s)	249, 654, 873, 1136
ErhI	CCWWGG	3 cut(s)	70, 269, 1791
FalI	AAGNNNNNCTT	2 cut(s)	1195, 1227
FaqI	GGGAC	3 cut(s)	501, 1108, 1310
FauNDI	CATATG	1 cut(s)	1187
FbaI	TGATCA	2 cut(s)	178, 1440
FblI	GTMKAC	1 cut(s)	793
Fnu4HI	GCNGC	9 cut(s)	9, 521, 945, 991, 1458, 1623, 1719, 1749, 1752
FriOI	GRGCYC	4 cut(s)	249, 654, 873, 1136
Fsp4HI	GCNGC	9 cut(s)	9, 521, 945, 991, 1458, 1623, 1719, 1749, 1752
FspBI	CTAG	2 cut(s)	692, 1461
GlaI	GCGC	3 cut(s)	124, 294, 424
GluI	GCNGC	9 cut(s)	9, 521, 945, 991, 1458, 1623, 1719, 1749, 1752
GsuI	CTGGAG	1 cut(s)	1782
HaeII	RGCGCY	1 cut(s)	296
HapII	CCGG	7 cut(s)	120, 243, 420, 452, 584, 1000, 1500
HgaI	GACGC	1 cut(s)	1312
HhaI	GCGC	3 cut(s)	125, 295, 425
Hin1I	GRCGYC	2 cut(s)	347, 1323
Hin6I	GCGC	3 cut(s)	123, 293, 423
HinP1I	GCGC	3 cut(s)	123, 293, 423
HindIII	AAGCTT	1 cut(s)	202
HinfI	GANTC	6 cut(s)	778, 889, 950, 1206, 1733, 1787
HpaII	CCGG	7 cut(s)	120, 243, 420, 452, 584, 1000, 1500
HphI	GGTGA	5 cut(s)	10, 346, 442, 1025, 1192
Hpy166II	GTNNAC	2 cut(s)	18, 794
Hpy188III	TCNNGA	3 cut(s)	232, 319, 744
Hpy8I	GTNNAC	2 cut(s)	18, 794
Hpy99I	CGWCG	4 cut(s)	130, 352, 551, 601
HpyAV	CCTTC	3 cut(s)	478, 1531, 1542
HpyCH4III	ACNGT	2 cut(s)	190, 601
HpyCH4IV	ACGT	6 cut(s)	128, 347, 407, 549, 633, 1576
HpyF10VI	GCNNNNNNNGC	5 cut(s)	953, 996, 1267, 1628, 1724
HpyF3I	CTNAG	7 cut(s)	12, 200, 500, 800, 867, 885, 1828
HpySE526I	ACGT	6 cut(s)	128, 347, 407, 549, 633, 1576
Hsp92I	GRCGYC	2 cut(s)	347, 1323
HspAI	GCGC	3 cut(s)	123, 293, 423
KroI	GCCGGC	1 cut(s)	999
KroNI	GCCGGC	1 cut(s)	1001
Ksp22I	TGATCA	2 cut(s)	178, 1440
Kzo9I	GATC	7 cut(s)	178, 234, 828, 859, 1059, 1410, 1440
LguI	GCTCTTC	1 cut(s)	530
LmnI	GCTCC	5 cut(s)	48, 907, 1131, 1641, 1801
Lsp1109I	GCAGC	8 cut(s)	532, 931, 977, 1444, 1609, 1730, 1735, 1738
LweI	GCATC	3 cut(s)	104, 1056, 1504
MaeI	CTAG	2 cut(s)	692, 1461
MaeII	ACGT	6 cut(s)	128, 347, 407, 549, 633, 1576
MaeIII	GTNAC	6 cut(s)	259, 334, 775, 783, 1142, 1207
MalI	GATC	7 cut(s)	180, 236, 830, 861, 1061, 1412, 1442
MboI	GATC	7 cut(s)	178, 234, 828, 859, 1059, 1410, 1440
MfeI	CAATTG	1 cut(s)	1532
MflI	RGATCY	2 cut(s)	828, 1410
MhlI	GDGCHC	5 cut(s)	249, 654, 873, 1136, 1378
MlsI	TGGCCA	2 cut(s)	1302, 1384
MluNI	TGGCCA	2 cut(s)	1302, 1384
MlyI	GAGTC	4 cut(s)	772, 959, 1215, 1796
MmeI	TCCRAC	1 cut(s)	323
Mox20I	TGGCCA	2 cut(s)	1302, 1384
MroNI	GCCGGC	1 cut(s)	999
MroXI	GAANNNNTTC	2 cut(s)	305, 446
MscI	TGGCCA	2 cut(s)	1302, 1384
MseI	TTAA	3 cut(s)	284, 569, 1100
MslI	CAYNNNNRTG	3 cut(s)	1052, 1277, 1616
Msp20I	TGGCCA	2 cut(s)	1302, 1384
MspA1I	CMGCKG	1 cut(s)	1721
MspI	CCGG	7 cut(s)	120, 243, 420, 452, 584, 1000, 1500
MspR9I	CCNGG	2 cut(s)	244, 452
MunI	CAATTG	1 cut(s)	1532
MvnI	CGCG	2 cut(s)	125, 425
MwoI	GCNNNNNNNGC	5 cut(s)	953, 996, 1267, 1628, 1724
NaeI	GCCGGC	1 cut(s)	1001
NciI	CCSGG	2 cut(s)	244, 452
NcoI	CCATGG	2 cut(s)	70, 269
NdeI	CATATG	1 cut(s)	1187
NdeII	GATC	7 cut(s)	178, 234, 828, 859, 1059, 1410, 1440
NgoMIV	GCCGGC	1 cut(s)	999
NheI	GCTAGC	1 cut(s)	1460
NlaIV	GGNNCC	4 cut(s)	1262, 1643, 1770, 1797
NmeAIII	GCCGAG	2 cut(s)	666, 1237
NmuCI	GTSAC	3 cut(s)	334, 775, 1207
NspI	RCATGY	4 cut(s)	136, 364, 1057, 1252
NspV	TTCGAA	1 cut(s)	816
OliI	CACNNNNGTG	1 cut(s)	1277
PagI	TCATGA	1 cut(s)	318
PceI	AGGCCT	1 cut(s)	1523
PciI	ACATGT	2 cut(s)	132, 1248
PciSI	GCTCTTC	1 cut(s)	530
PdiI	GCCGGC	1 cut(s)	1001
PdmI	GAANNNNTTC	2 cut(s)	305, 446
PfeI	GAWTC	2 cut(s)	889, 1733
PfoI	TCCNGGA	1 cut(s)	450
PkrI	GCNGC	9 cut(s)	10, 522, 946, 992, 1459, 1624, 1720, 1750, 1753
PleI	GAGTC	4 cut(s)	772, 958, 1214, 1795
PpsI	GAGTC	4 cut(s)	772, 958, 1214, 1795
PscI	ACATGT	2 cut(s)	132, 1248
PsiI	TTATAA	1 cut(s)	1616
Psp124BI	GAGCTC	3 cut(s)	654, 873, 1136
PspEI	GGTNACC	1 cut(s)	1142
PspN4I	GGNNCC	4 cut(s)	1262, 1643, 1770, 1797
PspPI	GGNCC	2 cut(s)	266, 1260
PstI	CTGCAG	1 cut(s)	949
PstNI	CAGNNNCTG	1 cut(s)	953
PsuI	RGATCY	2 cut(s)	828, 1410
RsaI	GTAC	6 cut(s)	131, 314, 380, 632, 755, 1427
RsaNI	GTAC	6 cut(s)	130, 313, 379, 631, 754, 1426
RseI	CAYNNNNRTG	3 cut(s)	1052, 1277, 1616
SacI	GAGCTC	3 cut(s)	654, 873, 1136
SapI	GCTCTTC	1 cut(s)	530
SaqAI	TTAA	3 cut(s)	284, 569, 1100
SatI	GCNGC	9 cut(s)	9, 521, 945, 991, 1458, 1623, 1719, 1749, 1752
Sau3AI	GATC	7 cut(s)	178, 234, 828, 859, 1059, 1410, 1440
Sau96I	GGNCC	2 cut(s)	266, 1260
ScaI	AGTACT	1 cut(s)	1427
SchI	GAGTC	4 cut(s)	772, 959, 1215, 1796
ScrFI	CCNGG	2 cut(s)	244, 452
SduI	GDGCHC	5 cut(s)	249, 654, 873, 1136, 1378
SfaNI	GCATC	3 cut(s)	104, 1056, 1504
SfcI	CTRYAG	1 cut(s)	945
SfuI	TTCGAA	1 cut(s)	816
SgrAI	CRCCGGYG	1 cut(s)	419
SmiMI	CAYNNNNRTG	3 cut(s)	1052, 1277, 1616
SmlI	CTYRAG	2 cut(s)	653, 1421
SmoI	CTYRAG	2 cut(s)	653, 1421
SseBI	AGGCCT	1 cut(s)	1523
SsiI	CCGC	7 cut(s)	8, 332, 425, 935, 1104, 1517, 1721
SspMI	CTAG	2 cut(s)	692, 1461
SstI	GAGCTC	3 cut(s)	654, 873, 1136
StuI	AGGCCT	1 cut(s)	1523
StyD4I	CCNGG	2 cut(s)	242, 450
StyI	CCWWGG	3 cut(s)	70, 269, 1791
TaaI	ACNGT	2 cut(s)	190, 601
TaiI	ACGT	6 cut(s)	131, 350, 410, 552, 636, 1579
TaqI	TCGA	6 cut(s)	350, 596, 644, 816, 1050, 1110
TatI	WGTACW	3 cut(s)	378, 753, 1425
TauI	GCSGC	1 cut(s)	11
TfiI	GAWTC	2 cut(s)	889, 1733
Tru1I	TTAA	3 cut(s)	284, 569, 1100
Tru9I	TTAA	3 cut(s)	284, 569, 1100
TscAI	CASTG	2 cut(s)	472, 1806
TseFI	GTSAC	3 cut(s)	334, 775, 1207
TseI	GCWGC	8 cut(s)	520, 944, 990, 1457, 1622, 1718, 1748, 1751
Tsp45I	GTSAC	3 cut(s)	334, 775, 1207
TspDTI	ATGAA	5 cut(s)	73, 307, 314, 759, 1600
TspRI	CASTG	2 cut(s)	472, 1806
XapI	RAATTY	6 cut(s)	397, 445, 737, 1504, 1679, 1804
XceI	RCATGY	4 cut(s)	136, 364, 1057, 1252
XcmI	CCANNNNNNNNNTGG	2 cut(s)	536, 1114
XmiI	GTMKAC	1 cut(s)	793
XmnI	GAANNNNTTC	2 cut(s)	305, 446
XspI	CTAG	2 cut(s)	692, 1461
ZraI	GACGTC	1 cut(s)	348
ZrmI	AGTACT	1 cut(s)	1427

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.