Rorug01G0117400

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001999
Physical Location & Seq
Forward (+)
20617642 .. 20623032
5391 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0117400.1

Sequence Viewer

Length: 771 bp
ATGTCTTCAATTCTTCATTGGAAAGGAGGTTCACAGAAAAAGTTAGTCGCAACAGGTGCATCCCTGGGTTCATTTGTTCTCGTACTACTTGTTGCTGCAGTTTATCGTGTCTACAGTTCTGACAGGAAAGAAAAAGAGAATCAATTAAAGCTTGAAGTATTTTTAGAGGATTACAGAGCACTCAAACCAAGCAGATATTCTTATGCAGATATTAAGAGGATTACAAATCAATTCAAGGAAAAATTAGGCCAAGGAGCCTATGGGACTGTTTTTAAGGGAAAACTTTCTGCTGAATGTTTTGTTGCGGTGAAAGTCCTCAATAATACCAAGGGGAATGGGGAAGAGTTTGTAAATGAAGTAGGAACAATGGGACATATCCACCATGTCAATGTGGTTCGATTGTTTGGATTCTGCGCGGATGGATTTAGACGAGCTCTTGTTGATGACTTCTTACCTAATGGTTCACTACAAGATTTCTTATCATCAGCAGACAATAATAATTCTTTCCTGGGTTGGGACCATAACTTCAACGCAAAGATTTCTGATTTTGGTTTGGCCAAGTTATGTTCCAAGGATCAAAGTATAGTGTCAATGACTACCGCCAGGGGAACGATGGGGTACATTGCACCTGAAGTGTTCTCCAGGAATTTCGGAAATGTGTCCTATAAGTCAGATGTCTATCGCTATGGAATGGTACTGCTTGAGATTGTAGGAAGGAGAAAGAACATTGGTTCAACCACAGAGAACACCAATGAAGTTTACTACCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

256

Amino Acids

28.57

Weight (kDa)

9.19

Isoelectric Point (pI)

19.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 77 - 166 3.4e-17 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 78 - 162 1.2e-12 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 174 - 239 1.7e-10 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 174 - 244 5.7e-11 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 111
AccII CGCG 1 cut(s) 416
AciI CCGC 3 cut(s) 305, 416, 600
AclWI GGATC 1 cut(s) 582
AcoI YGGCCR 1 cut(s) 555
AcsI RAATTY 1 cut(s) 646
AcuI CTGAAG 1 cut(s) 651
AfaI GTAC 3 cut(s) 84, 620, 696
AfiI CCNNNNNNNGG 1 cut(s) 514
AgsI TTSAA 5 cut(s) 9, 155, 235, 529, 735
AjnI CCWGG 4 cut(s) 63, 507, 602, 641
AjuI GAANNNNNNNTTGG 2 cut(s) 181, 213
AloI GAACNNNNNNTCC 2 cut(s) 13, 45
AluBI AGCT 2 cut(s) 151, 434
AluI AGCT 2 cut(s) 151, 434
Alw21I GWGCWC 2 cut(s) 181, 436
AlwI GGATC 1 cut(s) 582
AoxI GGCC 2 cut(s) 247, 555
ApeKI GCWGC 1 cut(s) 95
ApoI RAATTY 1 cut(s) 646
Asp700I GAANNNNTTC 1 cut(s) 283
AspLEI GCGC 1 cut(s) 416
AspS9I GGNCC 1 cut(s) 517
AsuHPI GGTGA 1 cut(s) 319
AvaII GGWCC 1 cut(s) 517
BalI TGGCCA 1 cut(s) 557
BanII GRGCYC 1 cut(s) 436
Bbv12I GWGCWC 2 cut(s) 181, 436
BbvI GCAGC 1 cut(s) 82
BccI CCATC 2 cut(s) 413, 607
BciT130I CCWGG 4 cut(s) 65, 509, 604, 643
BfmI CTRYAG 2 cut(s) 96, 112
BisI GCNGC 1 cut(s) 96
BlsI GCNGC 1 cut(s) 97
Bme1390I CCNGG 4 cut(s) 65, 509, 604, 643
Bme18I GGWCC 1 cut(s) 517
BmgT120I GGNCC 1 cut(s) 517
BmiI GGNNCC 2 cut(s) 256, 518
BmrFI CCNGG 4 cut(s) 65, 509, 604, 643
BmsI GCATC 1 cut(s) 68
BpmI CTGGAG 1 cut(s) 625
BpuEI CTTGAG 1 cut(s) 722
BsaBI GATNNNNATC 1 cut(s) 678
BsaJI CCNNGG 7 cut(s) 63, 64, 250, 327, 508, 570, 603
Bsc4I CCNNNNNNNGG 1 cut(s) 514
Bse3DI GCAATG 1 cut(s) 621
Bse8I GATNNNNATC 1 cut(s) 678
BseBI CCWGG 4 cut(s) 65, 509, 604, 643
BseDI CCNNGG 7 cut(s) 63, 64, 250, 327, 508, 570, 603
BseGI GGATG 2 cut(s) 59, 424
BseJI GATNNNNATC 1 cut(s) 678
BseLI CCNNNNNNNGG 1 cut(s) 514
BseMI GCAATG 1 cut(s) 621
BseXI GCAGC 1 cut(s) 82
Bsh1236I CGCG 1 cut(s) 416
BshFI GGCC 2 cut(s) 249, 557
BsiHKAI GWGCWC 2 cut(s) 181, 436
BslFI GGGAC 3 cut(s) 277, 384, 530
BslI CCNNNNNNNGG 1 cut(s) 514
BsmFI GGGAC 3 cut(s) 277, 384, 530
BsnI GGCC 2 cut(s) 249, 557
Bsp1286I GDGCHC 2 cut(s) 181, 436
Bsp143I GATC 1 cut(s) 574
BspACI CCGC 3 cut(s) 305, 416, 600
BspANI GGCC 2 cut(s) 249, 557
BspFNI CGCG 1 cut(s) 416
BspLI GGNNCC 2 cut(s) 256, 518
BspMAI CTGCAG 1 cut(s) 100
BspPI GGATC 1 cut(s) 582
BsrDI GCAATG 1 cut(s) 621
BssECI CCNNGG 7 cut(s) 63, 64, 250, 327, 508, 570, 603
BssMI GATC 1 cut(s) 574
BssT1I CCWWGG 3 cut(s) 250, 327, 570
Bst2UI CCWGG 4 cut(s) 65, 509, 604, 643
Bst4CI ACNGT 2 cut(s) 116, 268
Bst6I CTCTTC 1 cut(s) 336
BstAPI GCANNNNNTGC 1 cut(s) 56
BstF5I GGATG 2 cut(s) 59, 424
BstFNI CGCG 1 cut(s) 416
BstHHI GCGC 1 cut(s) 416
BstKTI GATC 1 cut(s) 577
BstMBI GATC 1 cut(s) 574
BstMWI GCNNNNNNNGC 1 cut(s) 56
BstNI CCWGG 4 cut(s) 65, 509, 604, 643
BstSCI CCNGG 4 cut(s) 63, 507, 602, 641
BstSFI CTRYAG 2 cut(s) 96, 112
BstUI CGCG 1 cut(s) 416
BstV1I GCAGC 1 cut(s) 82
BsuRI GGCC 2 cut(s) 249, 557
BtsCI GGATG 2 cut(s) 59, 424
CfoI GCGC 1 cut(s) 416
Cfr13I GGNCC 1 cut(s) 517
Csp6I GTAC 3 cut(s) 83, 619, 695
CviAII CATG 1 cut(s) 383
CviJI RGCY 5 cut(s) 151, 249, 257, 434, 557
CviKI_1 RGCY 5 cut(s) 151, 249, 257, 434, 557
CviQI GTAC 3 cut(s) 83, 619, 695
DpnI GATC 1 cut(s) 576
DpnII GATC 1 cut(s) 574
EaeI YGGCCR 1 cut(s) 555
Eam1104I CTCTTC 1 cut(s) 336
EarI CTCTTC 1 cut(s) 336
Ecl136II GAGCTC 1 cut(s) 434
Eco130I CCWWGG 3 cut(s) 250, 327, 570
Eco24I GRGCYC 1 cut(s) 436
Eco47I GGWCC 1 cut(s) 517
Eco53kI GAGCTC 1 cut(s) 434
Eco57I CTGAAG 1 cut(s) 651
EcoICRI GAGCTC 1 cut(s) 434
EcoRII CCWGG 4 cut(s) 63, 507, 602, 641
EcoT14I CCWWGG 3 cut(s) 250, 327, 570
EcoT38I GRGCYC 1 cut(s) 436
ErhI CCWWGG 3 cut(s) 250, 327, 570
FaeI CATG 1 cut(s) 386
FaqI GGGAC 3 cut(s) 277, 384, 530
FatI CATG 1 cut(s) 382
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 1 cut(s) 96
FokI GGATG 2 cut(s) 46, 431
FriOI GRGCYC 1 cut(s) 436
Fsp4HI GCNGC 1 cut(s) 96
GlaI GCGC 1 cut(s) 415
GluI GCNGC 1 cut(s) 96
GsuI CTGGAG 1 cut(s) 625
HaeIII GGCC 2 cut(s) 249, 557
HhaI GCGC 1 cut(s) 416
Hin1II CATG 1 cut(s) 386
Hin6I GCGC 1 cut(s) 414
HinP1I GCGC 1 cut(s) 414
HindIII AAGCTT 1 cut(s) 149
HinfI GANTC 2 cut(s) 139, 408
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 4 cut(s) 32, 112, 464, 760
Hpy188I TCNGA 4 cut(s) 121, 544, 653, 673
Hpy8I GTNNAC 4 cut(s) 32, 112, 464, 760
HpyAV CCTTC 1 cut(s) 708
HpyCH4III ACNGT 2 cut(s) 116, 268
HpyCH4V TGCA 4 cut(s) 59, 98, 206, 626
HpyF10VI GCNNNNNNNGC 1 cut(s) 56
Hsp92II CATG 1 cut(s) 386
HspAI GCGC 1 cut(s) 414
Kzo9I GATC 1 cut(s) 574
LmnI GCTCC 1 cut(s) 254
Lsp1109I GCAGC 1 cut(s) 82
LweI GCATC 1 cut(s) 68
MalI GATC 1 cut(s) 576
MboI GATC 1 cut(s) 574
MboII GAAGA 2 cut(s) 5, 353
MhlI GDGCHC 2 cut(s) 181, 436
MlsI TGGCCA 1 cut(s) 557
MluCI AATT 6 cut(s) 9, 143, 230, 242, 499, 646
MluNI TGGCCA 1 cut(s) 557
MnlI CCTC 4 cut(s) 20, 160, 210, 326
Mox20I TGGCCA 1 cut(s) 557
MroXI GAANNNNTTC 1 cut(s) 283
MscI TGGCCA 1 cut(s) 557
MseI TTAA 3 cut(s) 146, 213, 273
MslI CAYNNNNRTG 1 cut(s) 387
Msp20I TGGCCA 1 cut(s) 557
MspR9I CCNGG 4 cut(s) 65, 509, 604, 643
MvaI CCWGG 4 cut(s) 65, 509, 604, 643
MvnI CGCG 1 cut(s) 416
MwoI GCNNNNNNNGC 1 cut(s) 56
NdeII GATC 1 cut(s) 574
NlaIII CATG 1 cut(s) 386
NlaIV GGNNCC 2 cut(s) 256, 518
PasI CCCWGGG 1 cut(s) 64
PdmI GAANNNNTTC 1 cut(s) 283
PfeI GAWTC 2 cut(s) 139, 408
PfoI TCCNGGA 1 cut(s) 641
PkrI GCNGC 1 cut(s) 97
Psp124BI GAGCTC 1 cut(s) 436
Psp6I CCWGG 4 cut(s) 63, 507, 602, 641
PspGI CCWGG 4 cut(s) 63, 507, 602, 641
PspN4I GGNNCC 2 cut(s) 256, 518
PspPI GGNCC 1 cut(s) 517
PstI CTGCAG 1 cut(s) 100
RsaI GTAC 3 cut(s) 84, 620, 696
RsaNI GTAC 3 cut(s) 83, 619, 695
RseI CAYNNNNRTG 1 cut(s) 387
SacI GAGCTC 1 cut(s) 436
SaqAI TTAA 3 cut(s) 146, 213, 273
SatI GCNGC 1 cut(s) 96
Sau3AI GATC 1 cut(s) 574
Sau96I GGNCC 1 cut(s) 517
ScrFI CCNGG 4 cut(s) 65, 509, 604, 643
SduI GDGCHC 2 cut(s) 181, 436
SetI ASST 6 cut(s) 31, 58, 153, 436, 457, 631
SfaNI GCATC 1 cut(s) 68
SfcI CTRYAG 2 cut(s) 96, 112
SinI GGWCC 1 cut(s) 517
SmiMI CAYNNNNRTG 1 cut(s) 387
SmlI CTYRAG 1 cut(s) 701
SmoI CTYRAG 1 cut(s) 701
Sse9I AATT 6 cut(s) 9, 143, 230, 242, 499, 646
SsiI CCGC 3 cut(s) 305, 416, 600
SstI GAGCTC 1 cut(s) 436
StyD4I CCNGG 4 cut(s) 63, 507, 602, 641
StyI CCWWGG 3 cut(s) 250, 327, 570
TaaI ACNGT 2 cut(s) 116, 268
TaqI TCGA 1 cut(s) 397
TasI AATT 6 cut(s) 9, 143, 230, 242, 499, 646
TfiI GAWTC 2 cut(s) 139, 408
Tru1I TTAA 3 cut(s) 146, 213, 273
Tru9I TTAA 3 cut(s) 146, 213, 273
TseI GCWGC 1 cut(s) 95
TspDTI ATGAA 4 cut(s) 5, 60, 369, 768
VpaK11BI GGWCC 1 cut(s) 517
XapI RAATTY 1 cut(s) 646
XcmI CCANNNNNNNNNTGG 2 cut(s) 257, 610
XmiI GTMKAC 1 cut(s) 111
XmnI GAANNNNTTC 1 cut(s) 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.