pycom07g05600

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001999
Physical Location & Seq
Forward (+)
4878518 .. 4888198
9681 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g05600.19

Sequence Viewer

Length: 1701 bp
ATGGATACAGTACAGTACATTAAAAATGAGCGAACAAAAGAAGCTGATCAAAATCTCACTTGTTGTTGCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCGTCGGCGGCGAAAATGCATTCGTCTCACTTGCTTCTGGAAGAGCCGATCAGGATGGCTTCGATCCTCGAGCCTTCCAAGCCTACATTCTTTCCGGCAATGACATCGATCGTGGGAACGCTTGGTCCAAAATCACAATCGGTTGAGACAATCTCCGGTTGCTTGAAGGCAGGAATGTCTGGTATTGCTCACCTGCTATGCATAATTTTCATTTTTCACCAAGAGACATTGGGAAACCTGAAGGTTGCAATGAAGAGTACCAATAAACTGTGTGCAGTAATGCTAGACACAGTTGGTCCAGAGTTGCAGGTAGTGAATAAAAGTGAGCATCCCATATCTCTTCAGGAAGATACCCTGGTTGTCCTAACACCTGATCAAGACCAAGAGGCCACCTCAAATCTACTACCCGTAAATTTTAGTGGCCTGGCAAAGGCAGTGAAGAAGGGAGATACAATTTTTATTGGTCAATACCTCTTTACGGGAAGTGAAACTACTTCTGTGTGGCTGGAGGTCACTGAGGTGAATGGTGAAGATGTGGTTTGTCTCATAAAGAATTCTGCTACCTTGGCAGGGTCATTGTATACTTTGAATGTCTCTCAGATTCGTATTGATCTGCCTACACTTACCGATAAAGATAAGGAGATCATAAGTACTTGGGGTGTTCGTAACACCATCGACTTCCTCTCATTATCGTATACGCGTCATGCTGAAGATGTTCGCCACGCCCGTGAGTTTCTTTCAAAGTTAGGCGACCTCAACCAAACTCAGATTTTTGCAAAAATTGAAAATGTAGAGGGGTTAAATCATTTTGATGAAATTTTGCAAGAAGCTGATGGTATTATCGTCTCTCGTGGAATTCTGGGGATCGATCTCCCACCAGAGAAGGTATTTTTATTTCAAAAGGCTGCTGTTTACAAGTGCAACATGGCTGGAAAGCCAGCAGTGGTGACTCGGGTTGTGGACAGCATGACCGACAACCTAAGACCTACTCGTGCTGAAGCAACTGATGTTGCCAACGCTGTACTGGATGGAAGTGATGCAATTCTTCTAGGTGCAGAGACCCTGCGCGGTTTGTACCCTGTTGAGACAATCTCCATTGTTGGAAAAATTTGCTCTGAGGCGGAGAAAGTTTTCAATCAAGATTTCTATTTTAAGAGGGCTGTCAAAAATGTTGGAGAACCAATGACCCACTTGGAATCTATTGCTTCCTCTGCGGTACGTGCGGCCATCAAGGTGAAGGCCTCTGTAATTATTTGCTTCACTACATCTGGAAGAGCTGCAAGGTTGATTGCAAAGTACAGACCAACAATGCCCGTGCTTTCTGTTGTCATTCCTCGGGTCAAGACAAATCAACTTCGATGGACATTTTCTGGTGCTTTTGAGGCAAGGCAATCACTCATTGTTAGAGGCATTTTTCCCATGCTAGCAGATCCTCGACATCCAGCTGAAACTACCGATGCAACAAATGAATCGATTCTTAAAATCGCTTTGGACCATGGCAAGGCCACTGGTGTCGTAAAATCACATGACCGTGTGGTCATTTGCCAAAAAATTGGCGATACTTCTGTGATCAAGATCATGGAGCATGAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

567

Amino Acids

61.66

Weight (kDa)

6.15

Isoelectric Point (pI)

30.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 306
AasI GACNNNNNNGTC 1 cut(s) 1640
Acc36I ACCTGC 2 cut(s) 306, 403
AccI GTMKAC 2 cut(s) 686, 800
AccII CGCG 2 cut(s) 805, 1173
AciI CCGC 5 cut(s) 113, 1173, 1226, 1319, 1328
AclWI GGATC 3 cut(s) 163, 977, 1529
AcoI YGGCCR 1 cut(s) 1329
AcsI RAATTY 5 cut(s) 517, 658, 921, 960, 1212
AcuI CTGAAG 4 cut(s) 365, 431, 834, 1122
AfaI GTAC 8 cut(s) 12, 17, 364, 757, 1128, 1181, 1323, 1403
AfiI CCNNNNNNNGG 4 cut(s) 535, 583, 675, 1606
AflIII ACRYGT 1 cut(s) 803
AgsI TTSAA 6 cut(s) 271, 694, 846, 890, 1004, 1240
AjnI CCWGG 2 cut(s) 459, 528
AjuI GAANNNNNNNTTGG 2 cut(s) 176, 208
AleI CACNNNNGTG 2 cut(s) 623, 831
AloI GAACNNNNNNTCC 2 cut(s) 214, 246
AluBI AGCT 4 cut(s) 44, 935, 1382, 1550
AluI AGCT 4 cut(s) 44, 935, 1382, 1550
Alw26I GTCTC 8 cut(s) 135, 245, 323, 653, 703, 955, 1157, 1184
AlwI GGATC 3 cut(s) 163, 977, 1529
Ama87I CYCGRG 3 cut(s) 173, 1056, 1440
AoxI GGCC 5 cut(s) 492, 526, 1329, 1344, 1608
ApeKI GCWGC 2 cut(s) 1010, 1382
ApoI RAATTY 5 cut(s) 517, 658, 921, 960, 1212
Asp700I GAANNNNTTC 3 cut(s) 819, 1235, 1578
AspLEI GCGC 1 cut(s) 1173
AspS9I GGNCC 3 cut(s) 230, 401, 1597
AsuHPI GGTGA 6 cut(s) 287, 314, 637, 644, 1063, 1351
AsuNHI GCTAGC 1 cut(s) 1528
AvaI CYCGRG 3 cut(s) 173, 1056, 1440
AvaII GGWCC 3 cut(s) 230, 401, 1597
BaeI ACNNNNGTAYC 4 cut(s) 447, 480, 1656, 1689
BarI GAAGNNNNNNTAC 2 cut(s) 580, 612
BauI CACGAG 2 cut(s) 954, 1095
BbvI GCAGC 2 cut(s) 997, 1369
BccI CCATC 6 cut(s) 154, 785, 932, 1127, 1340, 1458
BcgI CGANNNNNNTGC 2 cut(s) 192, 226
BciT130I CCWGG 2 cut(s) 461, 530
BclI TGATCA 3 cut(s) 46, 478, 1674
BcoDI GTCTC 8 cut(s) 135, 245, 323, 653, 703, 955, 1157, 1184
BfaI CTAG 3 cut(s) 389, 1154, 1529
BfuAI ACCTGC 2 cut(s) 306, 403
BisI GCNGC 4 cut(s) 114, 1011, 1329, 1383
BlsI GCNGC 4 cut(s) 115, 1012, 1330, 1384
BmcAI AGTACT 1 cut(s) 757
Bme1390I CCNGG 2 cut(s) 461, 530
Bme18I GGWCC 3 cut(s) 230, 401, 1597
BmeT110I CYCGRG 3 cut(s) 173, 1056, 1440
BmgT120I GGNCC 3 cut(s) 230, 401, 1597
BmrFI CCNGG 2 cut(s) 461, 530
BmsI GCATC 3 cut(s) 442, 1132, 1552
BmtI GCTAGC 1 cut(s) 1532
BplI GAGNNNNNCTC 6 cut(s) 242, 274, 482, 514, 1181, 1213
BpmI CTGGAG 1 cut(s) 632
Bsa29I ATCGAT 3 cut(s) 212, 972, 1577
BsaAI YACGTR 1 cut(s) 1325
BsaBI GATNNNNATC 2 cut(s) 51, 1679
BsaI GGTCTC 1 cut(s) 1157
BsaJI CCNNGG 4 cut(s) 459, 669, 1439, 1600
BsaWI WCCGGW 1 cut(s) 260
Bsc4I CCNNNNNNNGG 4 cut(s) 535, 583, 675, 1606
Bse1I ACTGG 2 cut(s) 1134, 1618
Bse3DI GCAATG 2 cut(s) 210, 360
Bse8I GATNNNNATC 2 cut(s) 51, 1679
BseBI CCWGG 2 cut(s) 461, 530
BseCI ATCGAT 3 cut(s) 212, 972, 1577
BseDI CCNNGG 4 cut(s) 459, 669, 1439, 1600
BseGI GGATG 4 cut(s) 165, 433, 1138, 1543
BseJI GATNNNNATC 2 cut(s) 51, 1679
BseLI CCNNNNNNNGG 4 cut(s) 535, 583, 675, 1606
BseMI GCAATG 2 cut(s) 210, 360
BseMII CTCAG 4 cut(s) 612, 716, 884, 1212
BseNI ACTGG 2 cut(s) 1134, 1618
BseXI GCAGC 2 cut(s) 997, 1369
BsgI GTGCAG 2 cut(s) 399, 1179
Bsh1236I CGCG 2 cut(s) 805, 1173
Bsh1285I CGRYCG 1 cut(s) 216
BshFI GGCC 5 cut(s) 494, 528, 1331, 1346, 1610
BshVI ATCGAT 3 cut(s) 212, 972, 1577
BsiEI CGRYCG 1 cut(s) 216
BsiHKCI CYCGRG 3 cut(s) 173, 1056, 1440
BsiSI CCGG 2 cut(s) 200, 261
BslI CCNNNNNNNGG 4 cut(s) 535, 583, 675, 1606
BsmAI GTCTC 8 cut(s) 135, 245, 323, 653, 703, 955, 1157, 1184
BsmBI CGTCTC 2 cut(s) 135, 955
BsmI GAATGC 1 cut(s) 124
BsnI GGCC 5 cut(s) 494, 528, 1331, 1346, 1610
Bso31I GGTCTC 1 cut(s) 1157
BsoBI CYCGRG 3 cut(s) 173, 1056, 1440
Bsp19I CCATGG 1 cut(s) 1600
BspACI CCGC 5 cut(s) 113, 1173, 1226, 1319, 1328
BspANI GGCC 5 cut(s) 494, 528, 1331, 1346, 1610
BspCNI CTCAG 4 cut(s) 613, 715, 883, 1213
BspDI ATCGAT 3 cut(s) 212, 972, 1577
BspFNI CGCG 2 cut(s) 805, 1173
BspMI ACCTGC 2 cut(s) 306, 403
BspOI GCTAGC 1 cut(s) 1532
BspPI GGATC 3 cut(s) 163, 977, 1529
BspQI GCTCTTC 3 cut(s) 76, 141, 1372
BspTNI GGTCTC 1 cut(s) 1157
BsrDI GCAATG 2 cut(s) 210, 360
BsrI ACTGG 2 cut(s) 1134, 1618
BssECI CCNNGG 4 cut(s) 459, 669, 1439, 1600
BssNAI GTATAC 2 cut(s) 687, 801
BssSI CACGAG 2 cut(s) 954, 1095
BssT1I CCWWGG 2 cut(s) 669, 1600
Bst1107I GTATAC 2 cut(s) 687, 801
Bst2BI CACGAG 2 cut(s) 954, 1095
Bst2UI CCWGG 2 cut(s) 461, 530
Bst4CI ACNGT 5 cut(s) 10, 15, 375, 397, 1637
Bst6I CTCTTC 5 cut(s) 76, 141, 353, 450, 1372
BstBAI YACGTR 1 cut(s) 1325
BstC8I GCNNGC 2 cut(s) 1044, 1530
BstDEI CTNAG 5 cut(s) 621, 702, 870, 1085, 1221
BstDSI CCRYGG 1 cut(s) 1600
BstENI CCTNNNNNAGG 1 cut(s) 533
BstF5I GGATG 4 cut(s) 165, 433, 1138, 1543
BstFNI CGCG 2 cut(s) 805, 1173
BstHHI GCGC 1 cut(s) 1173
BstMAI GTCTC 8 cut(s) 135, 245, 323, 653, 703, 955, 1157, 1184
BstMCI CGRYCG 1 cut(s) 216
BstMWI GCNNNNNNNGC 5 cut(s) 184, 671, 1316, 1325, 1487
BstNI CCWGG 2 cut(s) 461, 530
BstSCI CCNGG 2 cut(s) 459, 528
BstUI CGCG 2 cut(s) 805, 1173
BstV1I GCAGC 2 cut(s) 997, 1369
BstX2I RGATCY 1 cut(s) 1534
BstXI CCANNNNNNTGG 1 cut(s) 1658
BstYI RGATCY 1 cut(s) 1534
BstZ17I GTATAC 2 cut(s) 687, 801
Bsu15I ATCGAT 3 cut(s) 212, 972, 1577
BsuRI GGCC 5 cut(s) 494, 528, 1331, 1346, 1610
BsuTUI ATCGAT 3 cut(s) 212, 972, 1577
BtgI CCRYGG 1 cut(s) 1600
BtsCI GGATG 4 cut(s) 165, 433, 1138, 1543
BtsI GCAGTG 2 cut(s) 546, 1053
BtsIMutI CAGTG 4 cut(s) 546, 618, 1053, 1611
BveI ACCTGC 2 cut(s) 306, 403
Cac8I GCNNGC 2 cut(s) 1044, 1530
CfoI GCGC 1 cut(s) 1173
Cfr13I GGNCC 3 cut(s) 230, 401, 1597
ClaI ATCGAT 3 cut(s) 212, 972, 1577
CseI GACGC 1 cut(s) 794
Csp6I GTAC 8 cut(s) 11, 16, 363, 756, 1127, 1180, 1322, 1402
CviAII CATG 8 cut(s) 809, 1030, 1072, 1525, 1601, 1631, 1684, 1691
CviQI GTAC 8 cut(s) 11, 16, 363, 756, 1127, 1180, 1322, 1402
DdeI CTNAG 5 cut(s) 621, 702, 870, 1085, 1221
DrdI GACNNNNNNGTC 1 cut(s) 1640
DseDI GACNNNNNNGTC 1 cut(s) 1640
EaeI YGGCCR 1 cut(s) 1329
Eam1104I CTCTTC 5 cut(s) 76, 141, 353, 450, 1372
EarI CTCTTC 5 cut(s) 76, 141, 353, 450, 1372
EciI GGCGGA 1 cut(s) 1241
Eco130I CCWWGG 2 cut(s) 669, 1600
Eco147I AGGCCT 1 cut(s) 1346
Eco31I GGTCTC 1 cut(s) 1157
Eco47I GGWCC 3 cut(s) 230, 401, 1597
Eco57I CTGAAG 4 cut(s) 365, 431, 834, 1122
Eco88I CYCGRG 3 cut(s) 173, 1056, 1440
EcoNI CCTNNNNNAGG 1 cut(s) 533
EcoRI GAATTC 2 cut(s) 658, 960
EcoRII CCWGG 2 cut(s) 459, 528
EcoT14I CCWWGG 2 cut(s) 669, 1600
EcoT22I ATGCAT 2 cut(s) 126, 308
ErhI CCWWGG 2 cut(s) 669, 1600
Esp3I CGTCTC 2 cut(s) 135, 955
FaeI CATG 8 cut(s) 812, 1033, 1075, 1528, 1604, 1634, 1687, 1694
FatI CATG 8 cut(s) 808, 1029, 1071, 1524, 1600, 1630, 1683, 1690
FbaI TGATCA 3 cut(s) 46, 478, 1674
FblI GTMKAC 2 cut(s) 686, 800
Fnu4HI GCNGC 4 cut(s) 114, 1011, 1329, 1383
FokI GGATG 4 cut(s) 172, 420, 1145, 1530
Fsp4HI GCNGC 4 cut(s) 114, 1011, 1329, 1383
FspBI CTAG 3 cut(s) 389, 1154, 1529
GlaI GCGC 1 cut(s) 1172
GluI GCNGC 4 cut(s) 114, 1011, 1329, 1383
GsuI CTGGAG 1 cut(s) 632
HaeIII GGCC 5 cut(s) 494, 528, 1331, 1346, 1610
HapII CCGG 2 cut(s) 200, 261
HgaI GACGC 1 cut(s) 794
HhaI GCGC 1 cut(s) 1173
Hin1II CATG 8 cut(s) 812, 1033, 1075, 1528, 1604, 1634, 1687, 1694
Hin6I GCGC 1 cut(s) 1171
HinP1I GCGC 1 cut(s) 1171
HinfI GANTC 5 cut(s) 706, 1054, 1301, 1574, 1579
HpaII CCGG 2 cut(s) 200, 261
HphI GGTGA 6 cut(s) 287, 314, 637, 644, 1063, 1351
Hpy166II GTNNAC 4 cut(s) 687, 801, 1018, 1066
Hpy188I TCNGA 3 cut(s) 705, 873, 1222
Hpy188III TCNNGA 9 cut(s) 143, 157, 404, 449, 482, 1244, 1374, 1447, 1678
Hpy8I GTNNAC 4 cut(s) 687, 801, 1018, 1066
Hpy99I CGWCG 1 cut(s) 112
HpyAV CCTTC 6 cut(s) 189, 265, 340, 541, 982, 1336
HpyCH4III ACNGT 5 cut(s) 10, 15, 375, 397, 1637
HpyCH4IV ACGT 1 cut(s) 1324
HpyF10VI GCNNNNNNNGC 5 cut(s) 184, 671, 1316, 1325, 1487
HpyF3I CTNAG 5 cut(s) 621, 702, 870, 1085, 1221
HpySE526I ACGT 1 cut(s) 1324
Hsp92II CATG 8 cut(s) 812, 1033, 1075, 1528, 1604, 1634, 1687, 1694
HspAI GCGC 1 cut(s) 1171
Ksp22I TGATCA 3 cut(s) 46, 478, 1674
LguI GCTCTTC 3 cut(s) 76, 141, 1372
LmnI GCTCC 1 cut(s) 1687
Lsp1109I GCAGC 2 cut(s) 997, 1369
LweI GCATC 3 cut(s) 442, 1132, 1552
MaeI CTAG 3 cut(s) 389, 1154, 1529
MaeII ACGT 1 cut(s) 1324
MaeIII GTNAC 3 cut(s) 616, 770, 1051
MflI RGATCY 1 cut(s) 1534
MluI ACGCGT 1 cut(s) 803
MlyI GAGTC 1 cut(s) 1048
MmeI TCCRAC 2 cut(s) 1186, 1258
Mph1103I ATGCAT 2 cut(s) 126, 308
MroXI GAANNNNTTC 3 cut(s) 819, 1235, 1578
MseI TTAA 4 cut(s) 21, 905, 1257, 1584
MslI CAYNNNNRTG 5 cut(s) 623, 831, 915, 1337, 1635
MspA1I CMGCKG 1 cut(s) 1550
MspI CCGG 2 cut(s) 200, 261
MspR9I CCNGG 2 cut(s) 461, 530
Mva1269I GAATGC 1 cut(s) 124
MvaI CCWGG 2 cut(s) 461, 530
MvnI CGCG 2 cut(s) 805, 1173
MwoI GCNNNNNNNGC 5 cut(s) 184, 671, 1316, 1325, 1487
NcoI CCATGG 1 cut(s) 1600
NheI GCTAGC 1 cut(s) 1528
NlaIII CATG 8 cut(s) 812, 1033, 1075, 1528, 1604, 1634, 1687, 1694
NmuCI GTSAC 2 cut(s) 616, 1051
NsiI ATGCAT 2 cut(s) 126, 308
OliI CACNNNNGTG 2 cut(s) 623, 831
PaeR7I CTCGAG 1 cut(s) 173
PaqCI CACCTGC 1 cut(s) 306
PceI AGGCCT 1 cut(s) 1346
PciSI GCTCTTC 3 cut(s) 76, 141, 1372
PcsI WCGNNNNNNNCGW 2 cut(s) 113, 829
PctI GAATGC 1 cut(s) 124
PdmI GAANNNNTTC 3 cut(s) 819, 1235, 1578
PfeI GAWTC 4 cut(s) 706, 1301, 1574, 1579
PkrI GCNGC 4 cut(s) 115, 1012, 1330, 1384
Ple19I CGATCG 1 cut(s) 216
PleI GAGTC 1 cut(s) 1048
PpsI GAGTC 1 cut(s) 1048
Ppu21I YACGTR 1 cut(s) 1325
Psp6I CCWGG 2 cut(s) 459, 528
PspGI CCWGG 2 cut(s) 459, 528
PspPI GGNCC 3 cut(s) 230, 401, 1597
PspXI VCTCGAGB 1 cut(s) 173
PsuI RGATCY 1 cut(s) 1534
PvuI CGATCG 1 cut(s) 216
PvuII CAGCTG 1 cut(s) 1550
RsaI GTAC 8 cut(s) 12, 17, 364, 757, 1128, 1181, 1323, 1403
RsaNI GTAC 8 cut(s) 11, 16, 363, 756, 1127, 1180, 1322, 1402
RseI CAYNNNNRTG 5 cut(s) 623, 831, 915, 1337, 1635
SapI GCTCTTC 3 cut(s) 76, 141, 1372
SaqAI TTAA 4 cut(s) 21, 905, 1257, 1584
SatI GCNGC 4 cut(s) 114, 1011, 1329, 1383
Sau96I GGNCC 3 cut(s) 230, 401, 1597
ScaI AGTACT 1 cut(s) 757
SchI GAGTC 1 cut(s) 1048
ScrFI CCNGG 2 cut(s) 461, 530
SfaNI GCATC 3 cut(s) 442, 1132, 1552
Sfr274I CTCGAG 1 cut(s) 173
SinI GGWCC 3 cut(s) 230, 401, 1597
SlaI CTCGAG 1 cut(s) 173
SmiMI CAYNNNNRTG 5 cut(s) 623, 831, 915, 1337, 1635
SmlI CTYRAG 1 cut(s) 173
SmoI CTYRAG 1 cut(s) 173
SseBI AGGCCT 1 cut(s) 1346
SsiI CCGC 5 cut(s) 113, 1173, 1226, 1319, 1328
SspMI CTAG 3 cut(s) 389, 1154, 1529
StuI AGGCCT 1 cut(s) 1346
StyD4I CCNGG 2 cut(s) 459, 528
StyI CCWWGG 2 cut(s) 669, 1600
TaaI ACNGT 5 cut(s) 10, 15, 375, 397, 1637
TaiI ACGT 1 cut(s) 1327
TaqI TCGA 8 cut(s) 167, 174, 212, 780, 972, 1462, 1540, 1577
TaqII GACCGA 1 cut(s) 1091
TatI WGTACW 5 cut(s) 10, 15, 755, 1126, 1401
TauI GCSGC 2 cut(s) 116, 1331
TfiI GAWTC 4 cut(s) 706, 1301, 1574, 1579
Tru1I TTAA 4 cut(s) 21, 905, 1257, 1584
Tru9I TTAA 4 cut(s) 21, 905, 1257, 1584
TscAI CASTG 4 cut(s) 546, 625, 1053, 1618
TseFI GTSAC 2 cut(s) 616, 1051
TseI GCWGC 2 cut(s) 1010, 1382
Tsp45I GTSAC 2 cut(s) 616, 1051
TspDTI ATGAA 4 cut(s) 304, 371, 933, 1587
TspRI CASTG 4 cut(s) 546, 625, 1053, 1618
VpaK11BI GGWCC 3 cut(s) 230, 401, 1597
XagI CCTNNNNNAGG 1 cut(s) 533
XapI RAATTY 5 cut(s) 517, 658, 921, 960, 1212
XcmI CCANNNNNNNNNTGG 1 cut(s) 1126
XhoI CTCGAG 1 cut(s) 173
XmiI GTMKAC 2 cut(s) 686, 800
XmnI GAANNNNTTC 3 cut(s) 819, 1235, 1578
XspI CTAG 3 cut(s) 389, 1154, 1529
ZrmI AGTACT 1 cut(s) 757
Zsp2I ATGCAT 2 cut(s) 126, 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.