Rorug03G0119200

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001999
Physical Location & Seq
Forward (+)
9648839 .. 9650209
1371 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0119200.1

Sequence Viewer

Length: 1371 bp
ATGCCTTGTAGTCTACGTGTGCTTGACCTTCATTCTAACCAGCTCCAGGGGAAAATCCCAGTTCTCTCATCACCTCATGTTTTTGCTCTAGATTACTCAAGGAATAATTTCAGCTCTAGCATTCCGGATGACATCGGTGCATATTTCACTAGTAACACTATATTCTTCTCTGTTGCAAGCAATAACCTCCATGGGATCATTCCAAGGTCAATATGCAATTCACAATCTCTCGAGATACTTGATCTGTCCAACAATTCATTGAGCGGAAGCTTTCCTCAGTGCTTGACTACAATGAAAGCTCTTGCAGTGCTTAATTTGAGGAGAAACAACCTTACAGATTATATTGCTGATAAATTTCCTCAAAGTTGCAGCTTACAAAGTCTAGACTTAAGTGGAAATCAGATACAAGGACAATTTCCAAAATCTCTAACCAACTGCACGAAGCGAGAGGTTTTAATTCTTGGAAAAAATCAGATAACAGATGCCTTTCCATGCTTTTTGAAGAACATATCTACTTTGCGTGTCCTTGCTTTGCAATCCAATAAGTTTTATGGGCACATTGGATGTGCCAAGACTAATGGCACTTGGCCAATGCTTCAGATCATAGATTTAGCAAACAACAATTTTGATGGTGAAATACCAGGATCATCTTTGTCAACCTGGCAGGCAATGATGGCTAACAAAGATGATGCTGCATCAAAGCTCCATCACCTTCAATTTCACAACCAGTATTTTTGTCAGGATGCAATTACTGTGACTAGCAAAGGCATAGATATGGAGTTGGTAAAGGTTCTAACTGTCTTCACCTCAATTGACTTCTCATGCAACAAGTTCAATGGACCAATACCTGAGGAAATTGGAGAATTGAAATCCTTGCGTGTACTGAACCTGTCGGGTAATGCTTTTACAGGTGAAATCCCATCATCATTAGGAAACTTGCAACAACTCGAGTCCTTAGACCTCTCACAGAACAACCTGGGCGGCCAAATTCCACCACAGCTTGCAAAACTTACTTTCCTTTCATTCATGAATGTGTCATACAATCAACTGGTTGGGGAGATCCCAAAAGGTACTCAGATTTCAACATTTTCGAAAGCTTCATTTGTAGGTAATAAAGGATTATATGGGCCACCGTTGACAGCAGAAAACAGACCAGTGTTGTCACCACCAACATTAGAAGGAAGCCATCAAAATTCTGGAGACGAGATTTATTGGGGTCTAATCAGTGCTGAAATTGGATTTACATTTGGAATCGGAATGGCGATTGGATCACTTTTGTTTTGCAAGAGATGGAGAAATTGGTACTACAAAGCTATGTTTAACATCCTTGTTAAGATATTCCCTCAATTGGAAGAAAGATGTGGTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

456

Amino Acids

50.28

Weight (kDa)

7.05

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 58 - 111 2.7e-07 Leucine rich repeat
LRR_8 PF13855 76 - 135 4.7e-10 Leucine rich repeat
LRR_14 PF23598 274 - 349 1.6e-08 Leucine-rich repeat region
LRR_4 PF12799 290 - 326 9.2e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 264
AccI GTMKAC 1 cut(s) 13
AccIII TCCGGA 1 cut(s) 124
AciI CCGC 2 cut(s) 264, 981
AclWI GGATC 4 cut(s) 203, 652, 1054, 1276
AcoI YGGCCR 2 cut(s) 587, 982
AcsI RAATTY 3 cut(s) 353, 987, 1192
AcuI CTGAAG 1 cut(s) 581
AfaI GTAC 3 cut(s) 882, 1072, 1304
AfiI CCNNNNNNNGG 2 cut(s) 46, 1348
AflII CTTAAG 1 cut(s) 388
AflIII ACRYGT 1 cut(s) 16
AgsI TTSAA 5 cut(s) 502, 716, 835, 868, 1083
AhlI ACTAGT 1 cut(s) 149
AjnI CCWGG 4 cut(s) 45, 640, 659, 975
AluBI AGCT 9 cut(s) 43, 114, 270, 299, 372, 703, 1000, 1097, 1313
AluI AGCT 9 cut(s) 43, 114, 270, 299, 372, 703, 1000, 1097, 1313
Alw26I GTCTC 1 cut(s) 1194
AlwI GGATC 4 cut(s) 203, 652, 1054, 1276
Ama87I CYCGRG 2 cut(s) 230, 947
Aor13HI TCCGGA 1 cut(s) 124
AoxI GGCC 3 cut(s) 587, 982, 1127
ApeKI GCWGC 2 cut(s) 369, 692
ApoI RAATTY 3 cut(s) 353, 987, 1192
Asp700I GAANNNNTTC 1 cut(s) 107
AspS9I GGNCC 2 cut(s) 839, 1127
AsuHPI GGTGA 6 cut(s) 63, 644, 701, 796, 923, 1155
AsuII TTCGAA 1 cut(s) 1091
AvaI CYCGRG 2 cut(s) 230, 947
AvaII GGWCC 1 cut(s) 839
AxyI CCTNAGG 1 cut(s) 849
BaeGI GKGCMC 1 cut(s) 558
BalI TGGCCA 1 cut(s) 589
BbsI GAAGAC 1 cut(s) 793
BbvI GCAGC 2 cut(s) 381, 679
BccI CCATC 6 cut(s) 623, 667, 714, 928, 1194, 1284
BciT130I CCWGG 4 cut(s) 47, 642, 661, 977
BcoDI GTCTC 1 cut(s) 1194
BcuI ACTAGT 1 cut(s) 149
BfaI CTAG 5 cut(s) 89, 117, 150, 383, 759
BfrI CTTAAG 1 cut(s) 388
BisI GCNGC 3 cut(s) 370, 693, 982
BlsI GCNGC 3 cut(s) 371, 694, 983
Bme1390I CCNGG 4 cut(s) 47, 642, 661, 977
Bme18I GGWCC 1 cut(s) 839
BmeT110I CYCGRG 2 cut(s) 230, 947
BmgT120I GGNCC 2 cut(s) 839, 1127
BmrFI CCNGG 4 cut(s) 47, 642, 661, 977
BmrI ACTGGG 1 cut(s) 53
BmsI GCATC 4 cut(s) 472, 679, 704, 733
BmuI ACTGGG 1 cut(s) 53
BpiI GAAGAC 1 cut(s) 793
BpmI CTGGAG 2 cut(s) 29, 1218
Bpu14I TTCGAA 1 cut(s) 1091
BpuEI CTTGAG 1 cut(s) 82
BsaAI YACGTR 1 cut(s) 17
BsaJI CCNNGG 4 cut(s) 46, 190, 203, 976
BsaWI WCCGGW 1 cut(s) 124
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 1348
Bse1I ACTGG 4 cut(s) 59, 727, 1053, 1154
Bse21I CCTNAGG 1 cut(s) 849
Bse3DI GCAATG 1 cut(s) 675
BseAI TCCGGA 1 cut(s) 124
BseBI CCWGG 4 cut(s) 47, 642, 661, 977
BseDI CCNNGG 4 cut(s) 46, 190, 203, 976
BseGI GGATG 4 cut(s) 133, 569, 748, 1323
BseLI CCNNNNNNNGG 2 cut(s) 46, 1348
BseMI GCAATG 1 cut(s) 675
BseMII CTCAG 3 cut(s) 290, 840, 1088
BseNI ACTGG 4 cut(s) 59, 727, 1053, 1154
BseRI GAGGAG 1 cut(s) 334
BseSI GKGCMC 1 cut(s) 558
BseXI GCAGC 2 cut(s) 381, 679
BsgI GTGCAG 1 cut(s) 421
BshFI GGCC 3 cut(s) 589, 984, 1129
BsiHKCI CYCGRG 2 cut(s) 230, 947
BsiSI CCGG 1 cut(s) 125
BslI CCNNNNNNNGG 2 cut(s) 46, 1348
BsmAI GTCTC 1 cut(s) 1194
BsmBI CGTCTC 1 cut(s) 1194
BsmI GAATGC 1 cut(s) 120
BsnI GGCC 3 cut(s) 589, 984, 1129
BsoBI CYCGRG 2 cut(s) 230, 947
Bsp119I TTCGAA 1 cut(s) 1091
Bsp1286I GDGCHC 1 cut(s) 558
Bsp13I TCCGGA 1 cut(s) 124
Bsp143I GATC 6 cut(s) 195, 241, 600, 644, 1059, 1268
Bsp19I CCATGG 1 cut(s) 190
BspACI CCGC 2 cut(s) 264, 981
BspANI GGCC 3 cut(s) 589, 984, 1129
BspCNI CTCAG 3 cut(s) 289, 841, 1087
BspEI TCCGGA 1 cut(s) 124
BspHI TCATGA 1 cut(s) 1026
BspPI GGATC 4 cut(s) 203, 652, 1054, 1276
BspT104I TTCGAA 1 cut(s) 1091
BspTI CTTAAG 1 cut(s) 388
BsrBI CCGCTC 1 cut(s) 264
BsrDI GCAATG 1 cut(s) 675
BsrI ACTGG 4 cut(s) 59, 727, 1053, 1154
BssECI CCNNGG 4 cut(s) 46, 190, 203, 976
BssMI GATC 6 cut(s) 195, 241, 600, 644, 1059, 1268
BssT1I CCWWGG 2 cut(s) 190, 203
Bst2UI CCWGG 4 cut(s) 47, 642, 661, 977
Bst4CI ACNGT 3 cut(s) 754, 799, 1134
BstAFI CTTAAG 1 cut(s) 388
BstBAI YACGTR 1 cut(s) 17
BstBI TTCGAA 1 cut(s) 1091
BstC8I GCNNGC 3 cut(s) 178, 666, 1002
BstDEI CTNAG 4 cut(s) 276, 849, 955, 1074
BstDSI CCRYGG 1 cut(s) 190
BstF5I GGATG 4 cut(s) 133, 569, 748, 1323
BstKTI GATC 6 cut(s) 198, 244, 603, 647, 1062, 1271
BstMAI GTCTC 1 cut(s) 1194
BstMBI GATC 6 cut(s) 195, 241, 600, 644, 1059, 1268
BstMWI GCNNNNNNNGC 1 cut(s) 674
BstNI CCWGG 4 cut(s) 47, 642, 661, 977
BstSCI CCNGG 4 cut(s) 45, 640, 659, 975
BstSLI GKGCMC 1 cut(s) 558
BstV1I GCAGC 2 cut(s) 381, 679
BstV2I GAAGAC 1 cut(s) 793
BstX2I RGATCY 1 cut(s) 1059
BstYI RGATCY 1 cut(s) 1059
Bsu36I CCTNAGG 1 cut(s) 849
BsuRI GGCC 3 cut(s) 589, 984, 1129
BtgI CCRYGG 1 cut(s) 190
BtsCI GGATG 4 cut(s) 133, 569, 748, 1323
BtsI GCAGTG 1 cut(s) 312
BtsIMutI CAGTG 4 cut(s) 284, 312, 1161, 1231
Cac8I GCNNGC 3 cut(s) 178, 666, 1002
CciI TCATGA 1 cut(s) 1026
Cfr13I GGNCC 2 cut(s) 839, 1127
Csp6I GTAC 3 cut(s) 881, 1071, 1303
CviAII CATG 5 cut(s) 77, 191, 492, 822, 1027
CviQI GTAC 3 cut(s) 881, 1071, 1303
DdeI CTNAG 4 cut(s) 276, 849, 955, 1074
DpnI GATC 6 cut(s) 197, 243, 602, 646, 1061, 1270
DpnII GATC 6 cut(s) 195, 241, 600, 644, 1059, 1268
EaeI YGGCCR 2 cut(s) 587, 982
Eco130I CCWWGG 2 cut(s) 190, 203
Eco47I GGWCC 1 cut(s) 839
Eco57I CTGAAG 1 cut(s) 581
Eco81I CCTNAGG 1 cut(s) 849
Eco88I CYCGRG 2 cut(s) 230, 947
EcoRII CCWGG 4 cut(s) 45, 640, 659, 975
EcoT14I CCWWGG 2 cut(s) 190, 203
ErhI CCWWGG 2 cut(s) 190, 203
Esp3I CGTCTC 1 cut(s) 1194
FaeI CATG 5 cut(s) 80, 194, 495, 825, 1030
FatI CATG 5 cut(s) 76, 190, 491, 821, 1026
FblI GTMKAC 1 cut(s) 13
Fnu4HI GCNGC 3 cut(s) 370, 693, 982
FokI GGATG 4 cut(s) 140, 576, 755, 1310
Fsp4HI GCNGC 3 cut(s) 370, 693, 982
FspBI CTAG 5 cut(s) 89, 117, 150, 383, 759
GluI GCNGC 3 cut(s) 370, 693, 982
GsuI CTGGAG 2 cut(s) 29, 1218
HaeIII GGCC 3 cut(s) 589, 984, 1129
HapII CCGG 1 cut(s) 125
Hin1II CATG 5 cut(s) 80, 194, 495, 825, 1030
HincII GTYRAC 2 cut(s) 657, 1137
HindII GTYRAC 2 cut(s) 657, 1137
HindIII AAGCTT 2 cut(s) 268, 1095
HinfI GANTC 2 cut(s) 950, 1251
HpaII CCGG 1 cut(s) 125
HphI GGTGA 6 cut(s) 63, 644, 701, 796, 923, 1155
Hpy166II GTNNAC 4 cut(s) 14, 657, 881, 1137
Hpy188I TCNGA 5 cut(s) 402, 474, 600, 1077, 1256
Hpy188III TCNNGA 8 cut(s) 89, 125, 230, 232, 383, 740, 1027, 1197
Hpy8I GTNNAC 4 cut(s) 14, 657, 881, 1137
HpyAV CCTTC 3 cut(s) 38, 722, 1172
HpyCH4III ACNGT 3 cut(s) 754, 799, 1134
HpyCH4IV ACGT 1 cut(s) 16
HpyF10VI GCNNNNNNNGC 1 cut(s) 674
HpyF3I CTNAG 4 cut(s) 276, 849, 955, 1074
HpySE526I ACGT 1 cut(s) 16
Hsp92II CATG 5 cut(s) 80, 194, 495, 825, 1030
Kpn2I TCCGGA 1 cut(s) 124
Kzo9I GATC 6 cut(s) 195, 241, 600, 644, 1059, 1268
LmnI GCTCC 2 cut(s) 48, 708
Lsp1109I GCAGC 2 cut(s) 381, 679
LweI GCATC 4 cut(s) 472, 679, 704, 733
MaeI CTAG 5 cut(s) 89, 117, 150, 383, 759
MaeII ACGT 1 cut(s) 16
MaeIII GTNAC 3 cut(s) 152, 754, 1161
MalI GATC 6 cut(s) 197, 243, 602, 646, 1061, 1270
MbiI CCGCTC 1 cut(s) 264
MboI GATC 6 cut(s) 195, 241, 600, 644, 1059, 1268
MboII GAAGA 4 cut(s) 157, 514, 793, 1364
MfeI CAATTG 2 cut(s) 810, 1346
MflI RGATCY 1 cut(s) 1059
MhlI GDGCHC 1 cut(s) 558
MlsI TGGCCA 1 cut(s) 589
MluNI TGGCCA 1 cut(s) 589
MlyI GAGTC 1 cut(s) 959
MmeI TCCRAC 1 cut(s) 273
Mox20I TGGCCA 1 cut(s) 589
MroI TCCGGA 1 cut(s) 124
MroXI GAANNNNTTC 1 cut(s) 107
MscI TGGCCA 1 cut(s) 589
MseI TTAA 6 cut(s) 312, 389, 455, 1320, 1332, 1369
MslI CAYNNNNRTG 2 cut(s) 773, 1031
Msp20I TGGCCA 1 cut(s) 589
MspCI CTTAAG 1 cut(s) 388
MspI CCGG 1 cut(s) 125
MspR9I CCNGG 4 cut(s) 47, 642, 661, 977
MunI CAATTG 2 cut(s) 810, 1346
Mva1269I GAATGC 1 cut(s) 120
MvaI CCWGG 4 cut(s) 47, 642, 661, 977
MwoI GCNNNNNNNGC 1 cut(s) 674
NcoI CCATGG 1 cut(s) 190
NdeII GATC 6 cut(s) 195, 241, 600, 644, 1059, 1268
NlaIII CATG 5 cut(s) 80, 194, 495, 825, 1030
NmuCI GTSAC 2 cut(s) 754, 1161
NspV TTCGAA 1 cut(s) 1091
PaeR7I CTCGAG 2 cut(s) 230, 947
PagI TCATGA 1 cut(s) 1026
PctI GAATGC 1 cut(s) 120
PdmI GAANNNNTTC 1 cut(s) 107
PfeI GAWTC 1 cut(s) 1251
PkrI GCNGC 3 cut(s) 371, 694, 983
PleI GAGTC 1 cut(s) 958
PpsI GAGTC 1 cut(s) 958
Ppu21I YACGTR 1 cut(s) 17
Psp6I CCWGG 4 cut(s) 45, 640, 659, 975
PspGI CCWGG 4 cut(s) 45, 640, 659, 975
PspPI GGNCC 2 cut(s) 839, 1127
PspXI VCTCGAGB 1 cut(s) 947
PsuI RGATCY 1 cut(s) 1059
RsaI GTAC 3 cut(s) 882, 1072, 1304
RsaNI GTAC 3 cut(s) 881, 1071, 1303
RseI CAYNNNNRTG 2 cut(s) 773, 1031
SaqAI TTAA 6 cut(s) 312, 389, 455, 1320, 1332, 1369
SatI GCNGC 3 cut(s) 370, 693, 982
Sau3AI GATC 6 cut(s) 195, 241, 600, 644, 1059, 1268
Sau96I GGNCC 2 cut(s) 839, 1127
SchI GAGTC 1 cut(s) 959
ScrFI CCNGG 4 cut(s) 47, 642, 661, 977
SduI GDGCHC 1 cut(s) 558
SfaNI GCATC 4 cut(s) 472, 679, 704, 733
Sfr274I CTCGAG 2 cut(s) 230, 947
SfuI TTCGAA 1 cut(s) 1091
SinI GGWCC 1 cut(s) 839
SlaI CTCGAG 2 cut(s) 230, 947
SmiMI CAYNNNNRTG 2 cut(s) 773, 1031
SmlI CTYRAG 4 cut(s) 97, 230, 388, 947
SmoI CTYRAG 4 cut(s) 97, 230, 388, 947
SpeI ACTAGT 1 cut(s) 149
SsiI CCGC 2 cut(s) 264, 981
SspMI CTAG 5 cut(s) 89, 117, 150, 383, 759
StyD4I CCNGG 4 cut(s) 45, 640, 659, 975
StyI CCWWGG 2 cut(s) 190, 203
TaaI ACNGT 3 cut(s) 754, 799, 1134
TaiI ACGT 1 cut(s) 19
TaqI TCGA 3 cut(s) 231, 948, 1091
TatI WGTACW 1 cut(s) 880
TauI GCSGC 1 cut(s) 984
TfiI GAWTC 1 cut(s) 1251
Tru1I TTAA 6 cut(s) 312, 389, 455, 1320, 1332, 1369
Tru9I TTAA 6 cut(s) 312, 389, 455, 1320, 1332, 1369
TscAI CASTG 4 cut(s) 284, 312, 1161, 1231
TseFI GTSAC 2 cut(s) 754, 1161
TseI GCWGC 2 cut(s) 369, 692
Tsp45I GTSAC 2 cut(s) 754, 1161
TspDTI ATGAA 7 cut(s) 20, 246, 308, 1011, 1015, 1043, 1089
TspRI CASTG 4 cut(s) 284, 312, 1161, 1231
Vha464I CTTAAG 1 cut(s) 388
VpaK11BI GGWCC 1 cut(s) 839
XapI RAATTY 3 cut(s) 353, 987, 1192
XbaI TCTAGA 2 cut(s) 88, 382
XcmI CCANNNNNNNNNTGG 1 cut(s) 1193
XhoI CTCGAG 2 cut(s) 230, 947
XmiI GTMKAC 1 cut(s) 13
XmnI GAANNNNTTC 1 cut(s) 107
XspI CTAG 5 cut(s) 89, 117, 150, 383, 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.