Rorug03G0119100

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001999
Physical Location & Seq
Forward (+)
9643906 .. 9647783
3878 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0119100.1

Sequence Viewer

Length: 3135 bp
ATGAAGACTCTGCTGCCTTTCTTCCTTTTCTTGATCGCTGCAGTTCACAGCCAGCAGTGTACCAAAGACCAGCAACTAACGTTGCTCGATTTCAAGAATGGCCTCGTTTTTAATTCTTCTCTTTCCTCCAAGCTTGTATCATGGAATTCAAGAACCGACTGCTGTTCTTGGTCGGGTGTAACTTGCAGTACTAACGGGACTGTTGTTGCTCTTGACATCAGCCGCGAGTCTATCTCAGGTGGAATCGACAACTCTAGCAGTTTGTTTGATCTTCAACATCTTCAAAGCCTCAGTTTGGCCTTTAACAACTTCACTGGCTCTCAAATTTCAACTGCAACCGGAAAGCTCACAAGTTTGAGGTACCTAAACTTATCCTCTGCTGGTTATGCAGGGCAAATTCCCATTGAGATTTCGTTGTTGGCAAAGTTGGTTATCCTTGATATTTCCAATCAAGGACTTGAGATCCAGAATTTGAGCTTGCTGGTTCAGAACCTCACGAAACTTGCAGAGTTATATCTTGACAATGTGAATATATTGTCACAGAGAAGTGACTGGTCCCTAGCCATATCGTCATCACTGCCAAACCTGAAGGTGTTGAGCTTATCCAACTGTCATCTTGCAGGCCCTATTGACAAGTCCCTGGCTAAGCTCTCATCTCTATCCGTGATTCGGTTGGATTTTAACAAGATATCTTCTCCCATTCCTGGATTCATTGCCAACTTTACAAACTTGACTACCTTGAGTCTCAGTAGCTGTGGTTTGCAGGGAACATTTCCAAAAGAGATCATTCAGGTACCTTCTTTACAAACTATTGATCTTTCCTATAACTATGGTCTTGGTGGTTCCTTGCCCGAATTTTCAAAGAATGCATCTCTTCAATCCTTGAATCTATTTGTGACAAACTTTTCAGGGGTCTTACCTGACTCCATTGGAAACCTCAGTATGTTGTCAACAATAGATCTTACAGGATGCAAATTTACTGGATCAATTCCAAGGTCAATGGGAAACCTTACAAAATTGGTTCATTTTGACTTGTCAGGGAATCAGTTTACTGGTTCAATTCCATGCTTCAGTAGCGCGAAAAATCTAGCCGAAATAAATCTGTCCGGCAATGATCTAACTGGTCATATTAGTTGCACTCAGTGGCAAAACCTTACTAGCCTAGTGACTATCAACTTAAGTAATAATATGCTCCAAGGGAACCTTTCATCCTCTTTGTTTTGTCTTCCCTTGCTGAAGAGCTTAGATCTTGGCCACAATCAATTCTCTGGTCAATTCCCTGAAATTTGCAATGCCTCTTCTTACTTGTTGGAAATTGTTGACTTAAGTGATAACAACCTTGAAGGGCCAATACCCATGTCTATCTTTAATCTCAGAGGACTTCAGAGACTTCTTCTTAATTCAAACAGTCTCAATGGCTCATTTCCTCTTGATGGTCTTCACAAGCTCATAAATCTTCAGTTTCTTTATCTTTCAGAGAATAGCTTGGTGCTCAGTTATGATGCTACCAATTCCTCTTATTCCTCATTTCCTCAGCTTTATCAATTGATGTTGGCTTCAGGAAAGTTGACAACATTCCCTGATTTCTTGAGAAATCAATCCGAATTGGCCTTTCTGGACCTTTCAAATAACCTGATACATGGCATGATACCTAACTGGCTTTGGAAGCTTAATCTTCATCATCTGAACCTTTCCTGTAACTCCCTACGAACTATAGAAGGTCCTTTACTCAATGTCACCTCTCCGTGGTTGCTGGACCTCAGTTACAACAGGCTTCAAGGGCAGATCCCAAATTTCTCTTCTTATTATCTGGATTGCTCCAGCAATAATTTCAGCTCTGGCATACCGACTGGTGCAGATTTCTCTAGGTATACTTCATTTTTCTCTGTTGGAAACAATAACCTCAATGGGATCATTCCCGGATCAATTTGCAATTCAGCAGCTCTTGAGGTTCTTGATCTATCCAATAATTCCTTCACTGGCTCTCTTCCTCAGTGTTTCACTACAATGAGTAGCCTAGCAGTACTTGATTTAAGTGGAAACAATGTTACAAATGTTCATGCATTACCTCAGAATTGTAACTTGGGAAGTCTAGACCTAAGTGGAAATCAGTTAGAAGGCCAGCTTCCAAAATCTCTTGTCAACTGCACAAAGTTGGAGGTTTTAAACCTCGGAAACAATCAGATAACGGATACTTTTCCATGCTTCTTGAAGAACATATCCACCTTGCGTCTCCTTAGTTTACGGTCCAATAACTTTTATGGACCCACTGGTTGTCCCAAGACTAATGGAGCTTGGCCAATGCTTCAGGTTATAGACCTAGCTGACAACAATTTTGATGGTGAAATATCTGGAAGATTTTTGTCAACCTGGCAAACAATGATAGCCAACAAAGATGATGCTGCATCAAATCGCTATTACCTTGGAATGCAGGGAGGTATGCATTATGTGATTAATTATCGAGATGCAGTTACAGTTAGTAGCAAAGGTTTACAGATGAACTTGGCAAAGATTCTAACTGTTTTCACCTTACTTGATTTCTCATGCAACAAGTTCAATGGATCAATACCAGAGGAAATTGGAGGACTCAAATCATTACGTGTCCTCAACTTGTCTAATAATGCTTTCACAGGTGCAATCCCATCATCATTAAGTAACTTGCAAGTAGTTGAGTCTTTAGACCTCTCGCAGAACAACCTGAGCGGTGAAATTCCACCACAGTTTGCGAAGCTTACTTTCCTTTCATTCTTGAATCTCTCAAATAACCAACTGGTGGGGAAGATTCCTACAAGTACTCAGTTTTCAACATTTCCAAAAACTTCATTTGAAGGAAATAAAGATCTATGGGGACCTCCTTTAACAGAAGAAAACACAGCGGGATCATCACCACCAACAATAGAAAAAAGTAGTCAAATTTCTGGACATGAGGTTGATTGGGATGTTATCAGTGTTGAAATTGGATTTGCATCTGGCTTTGGAGTTGCCATTGCGTCACTATTCTTATGCAAGAGATGGAGGCAGTGGTATTACATGGCTATGTATAACATGCTTGTTAGGATATTCCCTCAGCTAGAACAAAGATTTGGCAATCACAGAAGACATGTTTCTATCCATCAACGGTATGGGAGGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

1044

Amino Acids

114.28

Weight (kDa)

6.16

Isoelectric Point (pI)

37.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 23 - 63 3.7e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 80 - 177 1.4e-08 Leucine-rich repeat region
LRR_14 PF23598 288 - 422 4.9e-07 Leucine-rich repeat region
LRR_8 PF13855 630 - 682 2.7e-08 Leucine rich repeat
LRR_14 PF23598 833 - 923 1.5e-07 Leucine-rich repeat region
LRR_8 PF13855 869 - 924 2.7e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 360, 793
AccB1I GGYRCC 2 cut(s) 360, 793
AccB7I CCANNNNNTGG 1 cut(s) 2773
AccBSI CCGCTC 1 cut(s) 2703
AccI GTMKAC 1 cut(s) 1871
AccII CGCG 2 cut(s) 225, 1079
AciI CCGC 3 cut(s) 223, 2703, 2876
AclI AACGTT 1 cut(s) 80
AclWI GGATC 7 cut(s) 457, 991, 1780, 1919, 1930, 2569, 2887
AcoI YGGCCR 2 cut(s) 1252, 2297
AcuI CTGAAG 7 cut(s) 608, 1054, 1256, 1367, 1442, 1542, 2291
AdeI CACNNNGTG 1 cut(s) 1143
AfaI GTAC 6 cut(s) 61, 190, 362, 795, 2025, 2794
AfiI CCNNNNNNNGG 6 cut(s) 295, 669, 704, 1433, 1746, 2773
AflII CTTAAG 2 cut(s) 1177, 1324
AflIII ACRYGT 2 cut(s) 2599, 3100
AjnI CCWGG 3 cut(s) 639, 703, 2369
AjuI GAANNNNNNNTTGG 8 cut(s) 1783, 1815, 1958, 1990, 2066, 2098, 3066, 3098
AloI GAACNNNNNNTCC 2 cut(s) 1193, 1225
Alw21I GWGCWC 1 cut(s) 1494
Alw26I GTCTC 4 cut(s) 749, 1381, 1415, 2237
AlwI GGATC 7 cut(s) 457, 991, 1780, 1919, 1930, 2569, 2887
AlwNI CAGNNNCTG 1 cut(s) 753
AoxI GGCC 8 cut(s) 100, 297, 622, 1252, 1346, 1608, 2119, 2297
ApeKI GCWGC 4 cut(s) 13, 38, 1940, 2402
ArsI GACNNNNNNTTYG 2 cut(s) 2318, 2350
AseI ATTAAT 1 cut(s) 2454
Asp718I GGTACC 2 cut(s) 360, 793
AspLEI GCGC 1 cut(s) 1079
AspS9I GGNCC 9 cut(s) 555, 623, 1346, 1618, 1721, 1756, 2247, 2264, 2849
AsuC2I CCSGG 1 cut(s) 1920
AsuHPI GGTGA 5 cut(s) 1729, 2354, 2518, 2717, 2877
AvaII GGWCC 7 cut(s) 555, 1618, 1721, 1756, 2247, 2264, 2849
BalI TGGCCA 2 cut(s) 1254, 2299
BanI GGYRCC 2 cut(s) 360, 793
BarI GAAGNNNNNNTAC 4 cut(s) 1335, 1367, 1710, 1742
BbsI GAAGAC 4 cut(s) 11, 1217, 1430, 3103
Bbv12I GWGCWC 1 cut(s) 1494
BbvCI CCTCAGC 2 cut(s) 1533, 3066
BbvI GCAGC 3 cut(s) 25, 1952, 2389
BccI CCATC 5 cut(s) 1427, 2333, 2650, 3006, 3120
BciT130I CCWGG 3 cut(s) 641, 705, 2371
BciVI GTATCC 1 cut(s) 2185
BcnI CCSGG 1 cut(s) 1920
BcoDI GTCTC 4 cut(s) 749, 1381, 1415, 2237
BfmI CTRYAG 2 cut(s) 39, 1713
BfrI CTTAAG 2 cut(s) 1177, 1324
BfuI GTATCC 1 cut(s) 2185
BglII AGATCT 3 cut(s) 958, 1246, 2839
BisI GCNGC 5 cut(s) 14, 39, 223, 1941, 2403
BlpI GCTNAGC 1 cut(s) 645
BlsI GCNGC 5 cut(s) 15, 40, 224, 1942, 2404
BmcAI AGTACT 3 cut(s) 190, 2025, 2794
Bme1390I CCNGG 4 cut(s) 641, 705, 1920, 2371
Bme18I GGWCC 7 cut(s) 555, 1618, 1721, 1756, 2247, 2264, 2849
BmgT120I GGNCC 9 cut(s) 555, 623, 1346, 1618, 1721, 1756, 2247, 2264, 2849
BmiI GGNNCC 7 cut(s) 362, 557, 795, 844, 1202, 2266, 2850
BmrFI CCNGG 4 cut(s) 641, 705, 1920, 2371
BmsI GCATC 7 cut(s) 878, 959, 1492, 2389, 2414, 2455, 2975
BpiI GAAGAC 4 cut(s) 11, 1217, 1430, 3103
BplI GAGNNNNNCTC 2 cut(s) 218, 250
BpmI CTGGAG 1 cut(s) 1804
Bpu10I CCTNAGC 3 cut(s) 1533, 2699, 3066
Bpu1102I GCTNAGC 1 cut(s) 645
BpuEI CTTGAG 4 cut(s) 479, 760, 1609, 1967
BpuMI CCSGG 1 cut(s) 1920
BsaAI YACGTR 1 cut(s) 2600
BsaBI GATNNNNATC 1 cut(s) 2965
BsaJI CCNNGG 6 cut(s) 639, 992, 1195, 1745, 2170, 2422
BsaWI WCCGGW 1 cut(s) 338
Bsc4I CCNNNNNNNGG 6 cut(s) 295, 669, 704, 1433, 1746, 2773
Bse3DI GCAATG 4 cut(s) 711, 1117, 1297, 2985
Bse8I GATNNNNATC 1 cut(s) 2965
BseBI CCWGG 3 cut(s) 641, 705, 2371
BseDI CCNNGG 6 cut(s) 639, 992, 1195, 1745, 2170, 2422
BseGI GGATG 3 cut(s) 974, 1208, 2944
BseJI GATNNNNATC 1 cut(s) 2965
BseLI CCNNNNNNNGG 6 cut(s) 295, 669, 704, 1433, 1746, 2773
BseMI GCAATG 4 cut(s) 711, 1117, 1297, 2985
BseXI GCAGC 3 cut(s) 25, 1952, 2389
BsgI GTGCAG 2 cut(s) 1875, 2131
Bsh1236I CGCG 2 cut(s) 225, 1079
BshFI GGCC 8 cut(s) 102, 299, 624, 1254, 1348, 1610, 2121, 2299
BshNI GGYRCC 2 cut(s) 360, 793
BsiHKAI GWGCWC 1 cut(s) 1494
BsiSI CCGG 3 cut(s) 339, 1107, 1920
BslFI GGGAC 5 cut(s) 211, 541, 622, 2262, 2862
BslI CCNNNNNNNGG 6 cut(s) 295, 669, 704, 1433, 1746, 2773
BsmAI GTCTC 4 cut(s) 749, 1381, 1415, 2237
BsmBI CGTCTC 1 cut(s) 2237
BsmFI GGGAC 5 cut(s) 211, 541, 622, 2262, 2862
BsmI GAATGC 2 cut(s) 871, 2433
BsnI GGCC 8 cut(s) 102, 299, 624, 1254, 1348, 1610, 2121, 2299
Bsp1286I GDGCHC 1 cut(s) 1494
Bsp1720I GCTNAGC 1 cut(s) 645
BspACI CCGC 3 cut(s) 223, 2703, 2876
BspANI GGCC 8 cut(s) 102, 299, 624, 1254, 1348, 1610, 2121, 2299
BspFNI CGCG 2 cut(s) 225, 1079
BspLI GGNNCC 7 cut(s) 362, 557, 795, 844, 1202, 2266, 2850
BspMAI CTGCAG 1 cut(s) 43
BspPI GGATC 7 cut(s) 457, 991, 1780, 1919, 1930, 2569, 2887
BspQI GCTCTTC 1 cut(s) 1232
BspT107I GGYRCC 2 cut(s) 360, 793
BspTI CTTAAG 2 cut(s) 1177, 1324
BsrBI CCGCTC 1 cut(s) 2703
BsrDI GCAATG 4 cut(s) 711, 1117, 1297, 2985
BssECI CCNNGG 6 cut(s) 639, 992, 1195, 1745, 2170, 2422
BssNAI GTATAC 1 cut(s) 1872
BssT1I CCWWGG 3 cut(s) 992, 1195, 2422
Bst1107I GTATAC 1 cut(s) 1872
Bst2UI CCWGG 3 cut(s) 641, 705, 2371
Bst4CI ACNGT 8 cut(s) 202, 611, 1409, 2247, 2476, 2521, 2721, 3120
Bst6I CTCTTC 5 cut(s) 879, 1232, 1303, 1804, 1992
BstAFI CTTAAG 2 cut(s) 1177, 1324
BstBAI YACGTR 1 cut(s) 2600
BstC8I GCNNGC 4 cut(s) 53, 479, 622, 2123
BstDSI CCRYGG 1 cut(s) 1745
BstF5I GGATG 3 cut(s) 974, 1208, 2944
BstFNI CGCG 2 cut(s) 225, 1079
BstHHI GCGC 1 cut(s) 1079
BstMAI GTCTC 4 cut(s) 749, 1381, 1415, 2237
BstMWI GCNNNNNNNGC 4 cut(s) 386, 1074, 1666, 1780
BstNI CCWGG 3 cut(s) 641, 705, 2371
BstNSI RCATGY 2 cut(s) 3049, 3104
BstSCI CCNGG 4 cut(s) 639, 703, 1918, 2369
BstSFI CTRYAG 2 cut(s) 39, 1713
BstUI CGCG 2 cut(s) 225, 1079
BstV1I GCAGC 3 cut(s) 25, 1952, 2389
BstV2I GAAGAC 4 cut(s) 11, 1217, 1430, 3103
BstX2I RGATCY 5 cut(s) 462, 958, 1246, 1785, 2839
BstYI RGATCY 5 cut(s) 462, 958, 1246, 1785, 2839
BstZ17I GTATAC 1 cut(s) 1872
BsuI GTATCC 1 cut(s) 2185
BsuRI GGCC 8 cut(s) 102, 299, 624, 1254, 1348, 1610, 2121, 2299
BtgI CCRYGG 1 cut(s) 1745
BtsCI GGATG 3 cut(s) 974, 1208, 2944
BtsI GCAGTG 3 cut(s) 62, 575, 3026
BtsIMutI CAGTG 9 cut(s) 62, 312, 575, 1148, 1977, 2000, 2268, 2953, 3026
Cac8I GCNNGC 4 cut(s) 53, 479, 622, 2123
CaiI CAGNNNCTG 1 cut(s) 753
CfoI GCGC 1 cut(s) 1079
Cfr13I GGNCC 9 cut(s) 555, 623, 1346, 1618, 1721, 1756, 2247, 2264, 2849
CseI GACGC 2 cut(s) 2219, 2979
Csp6I GTAC 6 cut(s) 60, 189, 361, 794, 2024, 2793
CviQI GTAC 6 cut(s) 60, 189, 361, 794, 2024, 2793
DraI TTTAAA 1 cut(s) 2166
DraIII CACNNNGTG 1 cut(s) 1143
EaeI YGGCCR 2 cut(s) 1252, 2297
Eam1104I CTCTTC 5 cut(s) 879, 1232, 1303, 1804, 1992
EarI CTCTTC 5 cut(s) 879, 1232, 1303, 1804, 1992
Eco130I CCWWGG 3 cut(s) 992, 1195, 2422
Eco32I GATATC 1 cut(s) 690
Eco47I GGWCC 7 cut(s) 555, 1618, 1721, 1756, 2247, 2264, 2849
Eco57I CTGAAG 7 cut(s) 608, 1054, 1256, 1367, 1442, 1542, 2291
EcoO109I RGGNCCY 3 cut(s) 623, 1721, 2849
EcoRI GAATTC 1 cut(s) 145
EcoRII CCWGG 3 cut(s) 639, 703, 2369
EcoRV GATATC 1 cut(s) 690
EcoT14I CCWWGG 3 cut(s) 992, 1195, 2422
EcoT22I ATGCAT 3 cut(s) 871, 2065, 2445
ErhI CCWWGG 3 cut(s) 992, 1195, 2422
Esp3I CGTCTC 1 cut(s) 2237
FalI AAGNNNNNCTT 4 cut(s) 1188, 1220, 2109, 2141
FaqI GGGAC 5 cut(s) 211, 541, 622, 2262, 2862
FauI CCCGC 1 cut(s) 2869
FblI GTMKAC 1 cut(s) 1871
Fnu4HI GCNGC 5 cut(s) 14, 39, 223, 1941, 2403
FokI GGATG 3 cut(s) 981, 1195, 2951
Fsp4HI GCNGC 5 cut(s) 14, 39, 223, 1941, 2403
GlaI GCGC 1 cut(s) 1078
GluI GCNGC 5 cut(s) 14, 39, 223, 1941, 2403
GsuI CTGGAG 1 cut(s) 1804
HaeIII GGCC 8 cut(s) 102, 299, 624, 1254, 1348, 1610, 2121, 2299
HapII CCGG 3 cut(s) 339, 1107, 1920
HgaI GACGC 2 cut(s) 2219, 2979
HhaI GCGC 1 cut(s) 1079
Hin6I GCGC 1 cut(s) 1077
HinP1I GCGC 1 cut(s) 1077
HincII GTYRAC 5 cut(s) 951, 1321, 1569, 2143, 2367
HindII GTYRAC 5 cut(s) 951, 1321, 1569, 2143, 2367
HindIII AAGCTT 3 cut(s) 131, 1667, 2729
HpaII CCGG 3 cut(s) 339, 1107, 1920
HphI GGTGA 5 cut(s) 1729, 2354, 2518, 2717, 2877
Hpy188I TCNGA 9 cut(s) 489, 1376, 1386, 1477, 1603, 1686, 2073, 2174, 2184
HpyAV CCTTC 7 cut(s) 583, 807, 1337, 1712, 1984, 2111, 2822
HpyCH4III ACNGT 8 cut(s) 202, 611, 1409, 2247, 2476, 2521, 2721, 3120
HpyCH4IV ACGT 2 cut(s) 80, 2599
HpyF10VI GCNNNNNNNGC 4 cut(s) 386, 1074, 1666, 1780
HpySE526I ACGT 2 cut(s) 80, 2599
HspAI GCGC 1 cut(s) 1077
KpnI GGTACC 2 cut(s) 364, 797
LguI GCTCTTC 1 cut(s) 1232
LmnI GCTCC 3 cut(s) 1197, 1823, 2291
Lsp1109I GCAGC 3 cut(s) 25, 1952, 2389
LweI GCATC 7 cut(s) 878, 959, 1492, 2389, 2414, 2455, 2975
MaeII ACGT 2 cut(s) 80, 2599
MbiI CCGCTC 1 cut(s) 2703
MfeI CAATTG 1 cut(s) 1544
MflI RGATCY 5 cut(s) 462, 958, 1246, 1785, 2839
MhlI GDGCHC 1 cut(s) 1494
MlsI TGGCCA 2 cut(s) 1254, 2299
MluNI TGGCCA 2 cut(s) 1254, 2299
MlyI GAGTC 5 cut(s) 236, 751, 917, 2580, 2681
MmeI TCCRAC 5 cut(s) 630, 654, 1290, 1870, 2136
Mox20I TGGCCA 2 cut(s) 1254, 2299
Mph1103I ATGCAT 3 cut(s) 871, 2065, 2445
MscI TGGCCA 2 cut(s) 1254, 2299
MslI CAYNNNNRTG 2 cut(s) 2006, 3035
Msp20I TGGCCA 2 cut(s) 1254, 2299
MspA1I CMGCKG 1 cut(s) 2876
MspCI CTTAAG 2 cut(s) 1177, 1324
MspI CCGG 3 cut(s) 339, 1107, 1920
MspR9I CCNGG 4 cut(s) 641, 705, 1920, 2371
MunI CAATTG 1 cut(s) 1544
Mva1269I GAATGC 2 cut(s) 871, 2433
MvaI CCWGG 3 cut(s) 641, 705, 2371
MvnI CGCG 2 cut(s) 225, 1079
MwoI GCNNNNNNNGC 4 cut(s) 386, 1074, 1666, 1780
NciI CCSGG 1 cut(s) 1920
NlaIV GGNNCC 7 cut(s) 362, 557, 795, 844, 1202, 2266, 2850
NmuCI GTSAC 6 cut(s) 537, 548, 895, 1165, 1735, 2991
NsiI ATGCAT 3 cut(s) 871, 2065, 2445
NspI RCATGY 2 cut(s) 3049, 3104
PciI ACATGT 1 cut(s) 3100
PciSI GCTCTTC 1 cut(s) 1232
PctI GAATGC 2 cut(s) 871, 2433
PfeI GAWTC 8 cut(s) 243, 667, 708, 886, 1042, 2512, 2752, 2782
PflMI CCANNNNNTGG 1 cut(s) 2773
PfoI TCCNGGA 2 cut(s) 703, 1918
PkrI GCNGC 5 cut(s) 15, 40, 224, 1942, 2404
PleI GAGTC 5 cut(s) 235, 750, 917, 2580, 2680
PpsI GAGTC 5 cut(s) 235, 750, 917, 2580, 2680
Ppu21I YACGTR 1 cut(s) 2600
PpuMI RGGWCCY 2 cut(s) 1721, 2849
PscI ACATGT 1 cut(s) 3100
PshBI ATTAAT 1 cut(s) 2454
Psp1406I AACGTT 1 cut(s) 80
Psp5II RGGWCCY 2 cut(s) 1721, 2849
Psp6I CCWGG 3 cut(s) 639, 703, 2369
PspGI CCWGG 3 cut(s) 639, 703, 2369
PspN4I GGNNCC 7 cut(s) 362, 557, 795, 844, 1202, 2266, 2850
PspPI GGNCC 9 cut(s) 555, 623, 1346, 1618, 1721, 1756, 2247, 2264, 2849
PspPPI RGGWCCY 2 cut(s) 1721, 2849
PstI CTGCAG 1 cut(s) 43
PstNI CAGNNNCTG 1 cut(s) 753
PsuI RGATCY 5 cut(s) 462, 958, 1246, 1785, 2839
RsaI GTAC 6 cut(s) 61, 190, 362, 795, 2025, 2794
RsaNI GTAC 6 cut(s) 60, 189, 361, 794, 2024, 2793
RseI CAYNNNNRTG 2 cut(s) 2006, 3035
SapI GCTCTTC 1 cut(s) 1232
SatI GCNGC 5 cut(s) 14, 39, 223, 1941, 2403
Sau96I GGNCC 9 cut(s) 555, 623, 1346, 1618, 1721, 1756, 2247, 2264, 2849
ScaI AGTACT 3 cut(s) 190, 2025, 2794
SchI GAGTC 5 cut(s) 236, 751, 917, 2580, 2681
ScrFI CCNGG 4 cut(s) 641, 705, 1920, 2371
SduI GDGCHC 1 cut(s) 1494
SfaNI GCATC 7 cut(s) 878, 959, 1492, 2389, 2414, 2455, 2975
SfcI CTRYAG 2 cut(s) 39, 1713
SinI GGWCC 7 cut(s) 555, 1618, 1721, 1756, 2247, 2264, 2849
SmiMI CAYNNNNRTG 2 cut(s) 2006, 3035
SmlI CTYRAG 6 cut(s) 458, 739, 1177, 1324, 1588, 1946
SmoI CTYRAG 6 cut(s) 458, 739, 1177, 1324, 1588, 1946
SsiI CCGC 3 cut(s) 223, 2703, 2876
StyD4I CCNGG 4 cut(s) 639, 703, 1918, 2369
StyI CCWWGG 3 cut(s) 992, 1195, 2422
TaaI ACNGT 8 cut(s) 202, 611, 1409, 2247, 2476, 2521, 2721, 3120
TaiI ACGT 2 cut(s) 83, 2602
TaqI TCGA 3 cut(s) 87, 246, 2461
TatI WGTACW 3 cut(s) 188, 2023, 2792
TauI GCSGC 1 cut(s) 225
TfiI GAWTC 8 cut(s) 243, 667, 708, 886, 1042, 2512, 2752, 2782
TscAI CASTG 9 cut(s) 62, 319, 582, 1148, 1984, 2000, 2275, 2953, 3026
TseFI GTSAC 6 cut(s) 537, 548, 895, 1165, 1735, 2991
TseI GCWGC 4 cut(s) 13, 38, 1940, 2402
Tsp45I GTSAC 6 cut(s) 537, 548, 895, 1165, 1735, 2991
TspGWI ACGGA 3 cut(s) 652, 1734, 2204
TspRI CASTG 9 cut(s) 62, 319, 582, 1148, 1984, 2000, 2275, 2953, 3026
Van91I CCANNNNNTGG 1 cut(s) 2773
Vha464I CTTAAG 2 cut(s) 1177, 1324
VpaK11BI GGWCC 7 cut(s) 555, 1618, 1721, 1756, 2247, 2264, 2849
VspI ATTAAT 1 cut(s) 2454
XbaI TCTAGA 1 cut(s) 2092
XceI RCATGY 2 cut(s) 3049, 3104
XcmI CCANNNNNNNNNTGG 1 cut(s) 3119
XmiI GTMKAC 1 cut(s) 1871
ZrmI AGTACT 3 cut(s) 190, 2025, 2794
Zsp2I ATGCAT 3 cut(s) 871, 2065, 2445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.