pycom14g09200

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0001999
Physical Location & Seq
Forward (+)
10822964 .. 10828058
5095 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g09200.1

Sequence Viewer

Length: 1584 bp
ATGCATTCGAATCACCTTCTTCTGGAAGAGCCAATCCGGATGGCTTCGATTCTCGAGCCGTCCAAGGCTACTTTTTTCCCCGCAATGACGAAGATTGTGGGTACGCTCGGCCCCAAATCGCGATCCGTGGAGGTTATCGCTTCCTGCCTCAATGCTGGAATGTCTGTGGCCCGATTCGACTTCTCGTGGGGCAACCGGGAATATCACCAGGAGACTCTGGAGAATCTCAAGGCCGCTGTTAAGAGTACTAAGAAGCTTTGCGCTGTTATGTTGGATACTGTGGGTCCTGAGTTGCAAGTCGCCAACAAAACTGGGAATCCTATTGCACTCCTCGGAGATGGACTCGTTGTTCTGACGCCAGATCGCGGACAAGAACCATCCTCAGAGCTGTTGCCCATCAACTTCGATGGATTGGCAAAGGCGGTGAAGACGGGAGATACCATTTTTGTTGGTCAGTACCTGTTCACGGGAAGTGAAACGACTTCTGTATGGTTGGAGGTTTCTGAAATAAAAGGAGAAGATGTTGTTTGTTCCGTAAAGAATTCTGCAACATTGGCAGGTTCATTGTTCACTTTGCATGCATCTCAAATTCACATTGACTTGCCTACTCTCTCTGATCAAGATACTGAGGTTATAAGTAGTTGGGGGCTTCAAAACAAAATCGACTTCCTCTCATTGTCATATACGAGGCATGCAGAAGATGTCCGCCATGCTCGTAAGTTCCTTAATAAGTTGGGCGACCTTGGTCAGACACAAATTTTTGCTAAGATTGAGAATATGGAGGGTTTAACCCATTTTGATGAGATTCTGCAAGAAGCTGATGGCATCATCCTATCCCGTGGGAACCTGGGCATTGATCTCCCACCAGAGAAGGTCTTTTTATTTCAAAAGGCTGCCCTTTACAAGTGTAACATGGCTGGAAAGCCTGCTGTGGTTACTCGTGTTGTGGATAGTATGACTGACAACTTGAGGCCAACCCGTGCTGAAGCAACAGATGTTGCAAATGCTGTATTGGATGGAAGTGATGCAATTCTTCTGGGTGCCGAGACTCTTCGTGGTTTGTACCCTGTTGATACAATCTCCACTGTTGGTAGAATTTGTGCTGAGGCAGAGAAGGTTTTCAACCAAGACTTGTATTTTAAGAGGACTGTCAAATATGTTGGAGAGCCAATGACACACTTGGAATCTATTGCATCCTCCGCGGTACGAGCAGCGATTAAGGTGAAGGCATCTGTTATTATTTGCTTCACTTCTTCAGGAAGGGCTGCGAGATTGATTGCAAAGTATCGACCCACAATGCCAGTTCTTTCAGTTGTCATTCCCCGGCTCAAGACAAATCAGCTGAAGTGGAGCTTTACTGGAGCCTTCGAGGCAAGGCAATCACTTTTAGTCAGGGGTCTGTACCCTATGCTTGCTGATCCTCGGCATCCTGCCGAGTCTACCAGTGCAACAAACGAGTCAGTTCTGAAGGTTGCCCTTGATCATGGAAAGGCATCCGGAGTGGTGAAGTCACACGATCGAGTAGTCGTTTGCCAGAAAGTTGGTGATACCTCTGTGGTCAAGATTATAGAGCTTGAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

528

Amino Acids

57.35

Weight (kDa)

6.48

Isoelectric Point (pI)

29.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 30 - 360 1.5e-86 Pyruvate kinase, barrel domain
PK_C PF02887 395 - 522 1.2e-23 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 635
Acc36I ACCTGC 1 cut(s) 548
AccB1I GGYRCC 1 cut(s) 1040
AccI GTMKAC 1 cut(s) 1439
AccII CGCG 3 cut(s) 121, 366, 1202
AccIII TCCGGA 2 cut(s) 36, 1496
AciI CCGC 7 cut(s) 81, 234, 366, 422, 706, 1200, 1202
AclWI GGATC 2 cut(s) 117, 1412
AcsI RAATTY 4 cut(s) 541, 588, 756, 1095
AcuI CTGAAG 4 cut(s) 1005, 1239, 1364, 1487
AcyI GRCGYC 1 cut(s) 356
AfaI GTAC 6 cut(s) 103, 247, 458, 1064, 1206, 1403
AfiI CCNNNNNNNGG 3 cut(s) 22, 365, 466
AgsI TTSAA 4 cut(s) 653, 887, 1123, 1577
AjnI CCWGG 2 cut(s) 207, 846
AloI GAACNNNNNNTCC 2 cut(s) 333, 365
AluBI AGCT 6 cut(s) 256, 388, 818, 1342, 1353, 1573
AluI AGCT 6 cut(s) 256, 388, 818, 1342, 1353, 1573
Alw26I GTCTC 2 cut(s) 206, 1040
AlwI GGATC 2 cut(s) 117, 1412
AlwNI CAGNNNCTG 1 cut(s) 460
Ama87I CYCGRG 1 cut(s) 53
Aor13HI TCCGGA 2 cut(s) 36, 1496
AoxI GGCC 4 cut(s) 109, 168, 231, 971
ApeKI GCWGC 3 cut(s) 893, 1211, 1265
ApoI RAATTY 4 cut(s) 541, 588, 756, 1095
Asp700I GAANNNNTTC 1 cut(s) 1118
AspLEI GCGC 1 cut(s) 263
AspS9I GGNCC 3 cut(s) 110, 169, 284
AsuC2I CCSGG 2 cut(s) 197, 1324
AsuHPI GGTGA 6 cut(s) 5, 197, 436, 1234, 1516, 1556
AsuII TTCGAA 1 cut(s) 8
AvaI CYCGRG 1 cut(s) 53
AvaII GGWCC 1 cut(s) 284
BaeI ACNNNNGTAYC 4 cut(s) 615, 648, 1539, 1572
BanI GGYRCC 1 cut(s) 1040
BauI CACGAG 2 cut(s) 184, 939
BbsI GAAGAC 1 cut(s) 434
BbvCI CCTCAGC 1 cut(s) 1104
BbvI GCAGC 3 cut(s) 880, 1223, 1252
BccI CCATC 7 cut(s) 34, 332, 385, 401, 404, 815, 1010
BceAI ACGGC 1 cut(s) 43
BciT130I CCWGG 2 cut(s) 209, 848
BciVI GTATCC 1 cut(s) 268
BclI TGATCA 2 cut(s) 616, 1480
BcnI CCSGG 2 cut(s) 197, 1324
BcoDI GTCTC 2 cut(s) 206, 1040
BfuAI ACCTGC 1 cut(s) 548
BfuI GTATCC 1 cut(s) 268
BglI GCCNNNNNGGC 1 cut(s) 1370
BisI GCNGC 4 cut(s) 234, 894, 1212, 1266
BlsI GCNGC 4 cut(s) 235, 895, 1213, 1267
BmcAI AGTACT 1 cut(s) 247
Bme1390I CCNGG 4 cut(s) 197, 209, 848, 1324
Bme18I GGWCC 1 cut(s) 284
BmeT110I CYCGRG 1 cut(s) 53
BmgT120I GGNCC 3 cut(s) 110, 169, 284
BmiI GGNNCC 5 cut(s) 112, 285, 845, 1042, 1363
BmrFI CCNGG 4 cut(s) 197, 209, 848, 1324
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 7 cut(s) 590, 834, 1015, 1202, 1238, 1435, 1502
BmuI ACTGGG 1 cut(s) 321
BoxI GACNNNNGTC 1 cut(s) 744
BpiI GAAGAC 1 cut(s) 434
BplI GAGNNNNNCTC 2 cut(s) 327, 359
BpmI CTGGAG 2 cut(s) 239, 1380
Bpu10I CCTNAGC 1 cut(s) 1104
Bpu14I TTCGAA 1 cut(s) 8
BpuEI CTTGAG 3 cut(s) 212, 988, 1313
BpuMI CCSGG 2 cut(s) 197, 1324
BsaHI GRCGYC 1 cut(s) 356
BsaJI CCNNGG 9 cut(s) 63, 126, 331, 742, 838, 847, 1200, 1322, 1421
BsaWI WCCGGW 2 cut(s) 36, 1496
BsaXI ACNNNNNCTCC 2 cut(s) 507, 537
Bsc4I CCNNNNNNNGG 3 cut(s) 22, 365, 466
Bse1I ACTGG 4 cut(s) 316, 1301, 1363, 1443
Bse3DI GCAATG 1 cut(s) 90
BseAI TCCGGA 2 cut(s) 36, 1496
BseBI CCWGG 2 cut(s) 209, 848
BseDI CCNNGG 9 cut(s) 63, 126, 331, 742, 838, 847, 1200, 1322, 1421
BseGI GGATG 7 cut(s) 45, 377, 828, 1021, 1193, 1426, 1493
BseLI CCNNNNNNNGG 3 cut(s) 22, 365, 466
BseMI GCAATG 1 cut(s) 90
BseMII CTCAG 4 cut(s) 279, 396, 618, 1095
BseNI ACTGG 4 cut(s) 316, 1301, 1363, 1443
BseRI GAGGAG 1 cut(s) 320
BseXI GCAGC 3 cut(s) 880, 1223, 1252
Bsh1236I CGCG 3 cut(s) 121, 366, 1202
Bsh1285I CGRYCG 1 cut(s) 1519
BshFI GGCC 4 cut(s) 111, 170, 233, 973
BshNI GGYRCC 1 cut(s) 1040
BsiEI CGRYCG 1 cut(s) 1519
BsiHKCI CYCGRG 1 cut(s) 53
BsiSI CCGG 4 cut(s) 37, 196, 1324, 1497
BslI CCNNNNNNNGG 3 cut(s) 22, 365, 466
BsmAI GTCTC 2 cut(s) 206, 1040
BsmI GAATGC 1 cut(s) 4
BsnI GGCC 4 cut(s) 111, 170, 233, 973
BsoBI CYCGRG 1 cut(s) 53
Bsp119I TTCGAA 1 cut(s) 8
Bsp13I TCCGGA 2 cut(s) 36, 1496
Bsp143I GATC 7 cut(s) 122, 361, 616, 856, 1417, 1480, 1516
Bsp68I TCGCGA 1 cut(s) 121
BspACI CCGC 7 cut(s) 81, 234, 366, 422, 706, 1200, 1202
BspANI GGCC 4 cut(s) 111, 170, 233, 973
BspCNI CTCAG 4 cut(s) 280, 395, 619, 1096
BspEI TCCGGA 2 cut(s) 36, 1496
BspFNI CGCG 3 cut(s) 121, 366, 1202
BspLI GGNNCC 5 cut(s) 112, 285, 845, 1042, 1363
BspMI ACCTGC 1 cut(s) 548
BspPI GGATC 2 cut(s) 117, 1412
BspQI GCTCTTC 1 cut(s) 21
BspT104I TTCGAA 1 cut(s) 8
BspT107I GGYRCC 1 cut(s) 1040
BsrDI GCAATG 1 cut(s) 90
BsrI ACTGG 4 cut(s) 316, 1301, 1363, 1443
BssECI CCNNGG 9 cut(s) 63, 126, 331, 742, 838, 847, 1200, 1322, 1421
BssMI GATC 7 cut(s) 122, 361, 616, 856, 1417, 1480, 1516
BssNI GRCGYC 1 cut(s) 356
BssSI CACGAG 2 cut(s) 184, 939
BssT1I CCWWGG 2 cut(s) 63, 742
Bst2BI CACGAG 2 cut(s) 184, 939
Bst2UI CCWGG 2 cut(s) 209, 848
Bst4CI ACNGT 3 cut(s) 280, 1087, 1150
Bst6I CTCTTC 2 cut(s) 21, 1056
BstACI GRCGYC 1 cut(s) 356
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 4 cut(s) 579, 693, 927, 1413
BstDEI CTNAG 6 cut(s) 249, 288, 382, 627, 765, 1104
BstDSI CCRYGG 3 cut(s) 126, 838, 1200
BstF5I GGATG 7 cut(s) 45, 377, 828, 1021, 1193, 1426, 1493
BstFNI CGCG 3 cut(s) 121, 366, 1202
BstHHI GCGC 1 cut(s) 263
BstKTI GATC 7 cut(s) 125, 364, 619, 859, 1420, 1483, 1519
BstMAI GTCTC 2 cut(s) 206, 1040
BstMBI GATC 7 cut(s) 122, 361, 616, 856, 1417, 1480, 1516
BstMCI CGRYCG 1 cut(s) 1519
BstMWI GCNNNNNNNGC 4 cut(s) 554, 1199, 1208, 1370
BstNI CCWGG 2 cut(s) 209, 848
BstNSI RCATGY 2 cut(s) 581, 695
BstPAI GACNNNNGTC 1 cut(s) 744
BstSCI CCNGG 4 cut(s) 195, 207, 846, 1322
BstUI CGCG 3 cut(s) 121, 366, 1202
BstV1I GCAGC 3 cut(s) 880, 1223, 1252
BstV2I GAAGAC 1 cut(s) 434
BstXI CCANNNNNNTGG 1 cut(s) 1541
BsuI GTATCC 1 cut(s) 268
BsuRI GGCC 4 cut(s) 111, 170, 233, 973
BtgI CCRYGG 3 cut(s) 126, 838, 1200
BtsCI GGATG 7 cut(s) 45, 377, 828, 1021, 1193, 1426, 1493
BtsIMutI CAGTG 2 cut(s) 1083, 1450
BtuMI TCGCGA 1 cut(s) 121
BveI ACCTGC 1 cut(s) 548
Cac8I GCNNGC 4 cut(s) 579, 693, 927, 1413
CaiI CAGNNNCTG 1 cut(s) 460
CfoI GCGC 1 cut(s) 263
Cfr13I GGNCC 3 cut(s) 110, 169, 284
Cfr42I CCGCGG 1 cut(s) 1203
CseI GACGC 1 cut(s) 364
Csp6I GTAC 6 cut(s) 102, 246, 457, 1063, 1205, 1402
CviAII CATG 5 cut(s) 578, 692, 710, 913, 1484
CviQI GTAC 6 cut(s) 102, 246, 457, 1063, 1205, 1402
DdeI CTNAG 6 cut(s) 249, 288, 382, 627, 765, 1104
DpnI GATC 7 cut(s) 124, 363, 618, 858, 1419, 1482, 1518
DpnII GATC 7 cut(s) 122, 361, 616, 856, 1417, 1480, 1516
Eam1104I CTCTTC 2 cut(s) 21, 1056
EarI CTCTTC 2 cut(s) 21, 1056
EciI GGCGGA 1 cut(s) 695
Eco130I CCWWGG 2 cut(s) 63, 742
Eco47I GGWCC 1 cut(s) 284
Eco57I CTGAAG 4 cut(s) 1005, 1239, 1364, 1487
Eco88I CYCGRG 1 cut(s) 53
EcoO109I RGGNCCY 1 cut(s) 284
EcoRI GAATTC 1 cut(s) 541
EcoRII CCWGG 2 cut(s) 207, 846
EcoT14I CCWWGG 2 cut(s) 63, 742
EcoT22I ATGCAT 2 cut(s) 6, 583
ErhI CCWWGG 2 cut(s) 63, 742
FaeI CATG 5 cut(s) 581, 695, 713, 916, 1487
FalI AAGNNNNNCTT 2 cut(s) 1337, 1369
FatI CATG 5 cut(s) 577, 691, 709, 912, 1483
FauI CCCGC 1 cut(s) 88
FbaI TGATCA 2 cut(s) 616, 1480
FblI GTMKAC 1 cut(s) 1439
Fnu4HI GCNGC 4 cut(s) 234, 894, 1212, 1266
FokI GGATG 7 cut(s) 52, 364, 815, 1028, 1180, 1413, 1480
Fsp4HI GCNGC 4 cut(s) 234, 894, 1212, 1266
GlaI GCGC 1 cut(s) 262
GluI GCNGC 4 cut(s) 234, 894, 1212, 1266
GsuI CTGGAG 2 cut(s) 239, 1380
HaeIII GGCC 4 cut(s) 111, 170, 233, 973
HapII CCGG 4 cut(s) 37, 196, 1324, 1497
HgaI GACGC 1 cut(s) 364
HhaI GCGC 1 cut(s) 263
Hin1I GRCGYC 1 cut(s) 356
Hin1II CATG 5 cut(s) 581, 695, 713, 916, 1487
Hin6I GCGC 1 cut(s) 261
HinP1I GCGC 1 cut(s) 261
HindIII AAGCTT 1 cut(s) 254
HpaII CCGG 4 cut(s) 37, 196, 1324, 1497
HphI GGTGA 6 cut(s) 5, 197, 436, 1234, 1516, 1556
Hpy166II GTNNAC 3 cut(s) 465, 570, 1440
Hpy188I TCNGA 7 cut(s) 335, 354, 385, 505, 616, 750, 1467
Hpy8I GTNNAC 3 cut(s) 465, 570, 1440
HpyAV CCTTC 7 cut(s) 26, 865, 1108, 1219, 1254, 1375, 1462
HpyCH4III ACNGT 3 cut(s) 280, 1087, 1150
HpyF10VI GCNNNNNNNGC 4 cut(s) 554, 1199, 1208, 1370
HpyF3I CTNAG 6 cut(s) 249, 288, 382, 627, 765, 1104
Hsp92I GRCGYC 1 cut(s) 356
Hsp92II CATG 5 cut(s) 581, 695, 713, 916, 1487
HspAI GCGC 1 cut(s) 261
Kpn2I TCCGGA 2 cut(s) 36, 1496
Ksp22I TGATCA 2 cut(s) 616, 1480
KspI CCGCGG 1 cut(s) 1203
Kzo9I GATC 7 cut(s) 122, 361, 616, 856, 1417, 1480, 1516
LguI GCTCTTC 1 cut(s) 21
LmnI GCTCC 2 cut(s) 1350, 1361
Lsp1109I GCAGC 3 cut(s) 880, 1223, 1252
LweI GCATC 7 cut(s) 590, 834, 1015, 1202, 1238, 1435, 1502
MaeIII GTNAC 3 cut(s) 908, 934, 1509
MalI GATC 7 cut(s) 124, 363, 618, 858, 1419, 1482, 1518
MboI GATC 7 cut(s) 122, 361, 616, 856, 1417, 1480, 1516
MboII GAAGA 9 cut(s) 11, 38, 103, 439, 530, 710, 1025, 1043, 1245
MluCI AATT 5 cut(s) 541, 588, 756, 1029, 1095
MlyI GAGTC 5 cut(s) 208, 336, 1042, 1445, 1466
MmeI TCCRAC 3 cut(s) 252, 474, 1141
Mph1103I ATGCAT 2 cut(s) 6, 583
MroI TCCGGA 2 cut(s) 36, 1496
MroXI GAANNNNTTC 1 cut(s) 1118
MseI TTAA 5 cut(s) 240, 726, 788, 1140, 1218
MslI CAYNNNNRTG 1 cut(s) 798
MspA1I CMGCKG 3 cut(s) 236, 1202, 1342
MspI CCGG 4 cut(s) 37, 196, 1324, 1497
MspR9I CCNGG 4 cut(s) 197, 209, 848, 1324
Mva1269I GAATGC 1 cut(s) 4
MvaI CCWGG 2 cut(s) 209, 848
MvnI CGCG 3 cut(s) 121, 366, 1202
MwoI GCNNNNNNNGC 4 cut(s) 554, 1199, 1208, 1370
NciI CCSGG 2 cut(s) 197, 1324
NdeII GATC 7 cut(s) 122, 361, 616, 856, 1417, 1480, 1516
NlaIII CATG 5 cut(s) 581, 695, 713, 916, 1487
NlaIV GGNNCC 5 cut(s) 112, 285, 845, 1042, 1363
NmeAIII GCCGAG 4 cut(s) 87, 1069, 1402, 1459
NmuCI GTSAC 1 cut(s) 1509
NruI TCGCGA 1 cut(s) 121
NsiI ATGCAT 2 cut(s) 6, 583
NspI RCATGY 2 cut(s) 581, 695
NspV TTCGAA 1 cut(s) 8
PaeI GCATGC 2 cut(s) 581, 695
PaeR7I CTCGAG 1 cut(s) 53
PciSI GCTCTTC 1 cut(s) 21
PctI GAATGC 1 cut(s) 4
PdmI GAANNNNTTC 1 cut(s) 1118
PfeI GAWTC 7 cut(s) 10, 49, 174, 223, 316, 805, 1184
PkrI GCNGC 4 cut(s) 235, 895, 1213, 1267
Ple19I CGATCG 1 cut(s) 1519
PleI GAGTC 5 cut(s) 208, 336, 1042, 1444, 1465
PpsI GAGTC 5 cut(s) 208, 336, 1042, 1444, 1465
PpuMI RGGWCCY 1 cut(s) 284
PshAI GACNNNNGTC 1 cut(s) 744
PsiI TTATAA 1 cut(s) 635
Psp5II RGGWCCY 1 cut(s) 284
Psp6I CCWGG 2 cut(s) 207, 846
PspGI CCWGG 2 cut(s) 207, 846
PspN4I GGNNCC 5 cut(s) 112, 285, 845, 1042, 1363
PspPI GGNCC 3 cut(s) 110, 169, 284
PspPPI RGGWCCY 1 cut(s) 284
PstNI CAGNNNCTG 1 cut(s) 460
PvuI CGATCG 1 cut(s) 1519
PvuII CAGCTG 1 cut(s) 1342
RruI TCGCGA 1 cut(s) 121
RsaI GTAC 6 cut(s) 103, 247, 458, 1064, 1206, 1403
RsaNI GTAC 6 cut(s) 102, 246, 457, 1063, 1205, 1402
RseI CAYNNNNRTG 1 cut(s) 798
SacII CCGCGG 1 cut(s) 1203
SapI GCTCTTC 1 cut(s) 21
SaqAI TTAA 5 cut(s) 240, 726, 788, 1140, 1218
SatI GCNGC 4 cut(s) 234, 894, 1212, 1266
Sau3AI GATC 7 cut(s) 122, 361, 616, 856, 1417, 1480, 1516
Sau96I GGNCC 3 cut(s) 110, 169, 284
ScaI AGTACT 1 cut(s) 247
SchI GAGTC 5 cut(s) 208, 336, 1042, 1445, 1466
ScrFI CCNGG 4 cut(s) 197, 209, 848, 1324
SfaNI GCATC 7 cut(s) 590, 834, 1015, 1202, 1238, 1435, 1502
Sfr274I CTCGAG 1 cut(s) 53
Sfr303I CCGCGG 1 cut(s) 1203
SfuI TTCGAA 1 cut(s) 8
SgrBI CCGCGG 1 cut(s) 1203
SinI GGWCC 1 cut(s) 284
SlaI CTCGAG 1 cut(s) 53
SmiMI CAYNNNNRTG 1 cut(s) 798
SmlI CTYRAG 4 cut(s) 53, 227, 967, 1328
SmoI CTYRAG 4 cut(s) 53, 227, 967, 1328
SphI GCATGC 2 cut(s) 581, 695
Sse9I AATT 5 cut(s) 541, 588, 756, 1029, 1095
SsiI CCGC 7 cut(s) 81, 234, 366, 422, 706, 1200, 1202
StyD4I CCNGG 4 cut(s) 195, 207, 846, 1322
StyI CCWWGG 2 cut(s) 63, 742
TaaI ACNGT 3 cut(s) 280, 1087, 1150
TaqI TCGA 9 cut(s) 8, 47, 54, 177, 405, 663, 1288, 1368, 1519
TasI AATT 5 cut(s) 541, 588, 756, 1029, 1095
TatI WGTACW 1 cut(s) 245
TauI GCSGC 1 cut(s) 236
TfiI GAWTC 7 cut(s) 10, 49, 174, 223, 316, 805, 1184
Tru1I TTAA 5 cut(s) 240, 726, 788, 1140, 1218
Tru9I TTAA 5 cut(s) 240, 726, 788, 1140, 1218
TscAI CASTG 2 cut(s) 1090, 1450
TseFI GTSAC 1 cut(s) 1509
TseI GCWGC 3 cut(s) 893, 1211, 1265
Tsp45I GTSAC 1 cut(s) 1509
TspDTI ATGAA 1 cut(s) 552
TspGWI ACGGA 2 cut(s) 115, 523
TspRI CASTG 2 cut(s) 1090, 1450
VpaK11BI GGWCC 1 cut(s) 284
XapI RAATTY 4 cut(s) 541, 588, 756, 1095
XceI RCATGY 2 cut(s) 581, 695
XhoI CTCGAG 1 cut(s) 53
XmiI GTMKAC 1 cut(s) 1439
XmnI GAANNNNTTC 1 cut(s) 1118
ZrmI AGTACT 1 cut(s) 247
Zsp2I ATGCAT 2 cut(s) 6, 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.