Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0001999

Physical Location & Seq

Genomic Coordinates Forward (+)

14073777 .. 14079126

Gene Structure 5350 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1094200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1584 bp

ATGCATTCGAATCACCTTCTTCTGGAAGAGCCAATACGGATGGCTTCGATTCTCGAGCCGTCCAAGGCTACTTTTTTCCCCGCAATGACGAAGATTGTGGGTACGCTCGGCCCGAAATCGCGATCCGTGGAGGTTATCGCTTCCTGCCTCAATGCTGGAATGTCTGTGGCCCGATTCGACTTCTCGTGGGGCAACCGGGAATATCACCAGGAGACTCTGGAGAATCTCAAGGCCGCTGTTAAGAGTACTAAGAAGCTTTGCGCTGTTATGTTGGACACTGTGGGTCCTGAGTTGCAAGTCGCCAACAAAACTGGGAATCCTATTGCACTCCTCGGAGATGGACTCGTTGTTCTGACGCCAGATCGCGGACAAGAACCATCCTCAGAGCTGTTGCCCATCAACTTCGATGGATTGGCAAAGGCGGTGAAGACGGGAGATACCATTTTTGTTGGTCAGTACCTGTTCACGGGAAGTGAAACGACTTCTGTATGGTTGGAGGTTTCTGAAATAAAAGGAGAAGATGTCGTTTGTTCCGTAAAGAATTCTGCAACATTGGCAGGTTCATTGTTCACTTTGCATGCATCTCAAATTCACATTGACCTGCCTACTCTCTCCGATCAAGATAATGAGGTTATAAGTAGTTGGGGACTTCAAAACAAAATCGACTTCCTATCATTGTCATATACGAGGCATGCAGAAGATGTCCGCCACGCTCGTAAGTTCCTTAATAAGCTGGGTGACCTCGGTCAGACACAAATTTTTGCTAAGATTGAGAATATGGAGGGTTTAACCCATTTTGATGAGATTCTGCAAGAAGCTGATGGCATCATCCTATCCCGTGGGAACCTGGGCATTGATCTCCCACCAGAGAAGGTCTTTTTATTTCAAAAGGCTGCCCTTTACAAGTGTAACATGGCTGGAAAGCCTGCTGTGGTTACTCGTGTTGTCGATAGTATGACTGACAACTTGAGGCCAACCCGTGCTGAAGCAACGGATGTTGCAAATGCTGTATTGGATGGAAGTGATGCAATTCTTCTGGGTGCTGAGACTCTTCGTGGTTTGTACCCTGTTGATACAATCTCCACTGTTGGTAGAATTTGTGCTGAGGCAGAGAAGGTTTTCAACCAAGACTTGTATTTTAAGAGGACTGTCAAATATGTTGGAGAGCCAATGACACACTTGGAATCTATTGCGTCCTCCGCGGTACGAGCAGCGATTAAGGTGAAGGCATCTGTTATTATTTGCTTCACTTCTTCAGGAAGGGCTGCGAGATTGATTGCAAAGTATCGACCCACAATGCCAGTTCTTTCAGTTGTCGTTCCCCGGCTCAAGACAAATCAGCTTAAGTGGAGCTTTACTGGAGCCTTTGAGGCAAGGCAATCGCTTTTAGTCAGGGGTCTGTACCCTATGCTTGCTGATCCTCGACATCCTGCCGAATCTACCAGTGCAACAAACGAGTCAGTTCTGAAGGTTGCCCTTGATCATGGAAAGGCATCCGGAGTGGTGAAGTCACATGATCGAGTAGTCGTTTGCCAGAAAGTTGGTGATACCTCCGTGGTCAAGATTATAGAGCTTGAAGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230

Pfam Domains

Protein Families

PK PK_C

Protein Analysis

528

Amino Acids

57.35

Weight (kDa)

6.48

Isoelectric Point (pI)

30.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK	PF00224	30 - 360	1.5e-86	Pyruvate kinase, barrel domain
PK_C	PF02887	395 - 522	9.2e-24	Pyruvate kinase, alpha/beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001999)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36580 AT3G52990 AT3G52990
fragaria_vesca	FvH4_6g17480 FvH4_6g17480 FvH4_6g17480 FvH4_6g17480 FvH4_7g06490 FvH4_7g06490 FvH4_7g06490 FvH4_7g06490 FvH4_7g06490
malus_domestica	MD02G1244000.v1.1 MD07G1072900.v1.1 MD07G1073000.v1.1 MD12G1096900.v1.1 MD14G1094200.v1.1
prunus_persica	Prupe.2G091200_v2.0.a1 Prupe.2G091200_v2.0.a1 Prupe.2G091200_v2.0.a1 Prupe.7G120700_v2.0.a1 Prupe.7G120700_v2.0.a1
pyrus_communis	pycom07g05600 pycom07g05630 pycom12g09800 pycom14g09200
rosa_chinensis	RchiOBHm_Chr1g0334861 RchiOBHm_Chr3g0471721
rosa_laevigata	RLG00000024139 RLG00000029414
rosa_multiflora	Rmu_sc0000574.1_g000046 Rmu_sc0005631.1_g000026 Rmu_sc0026537.1_g000003
rosa_roxburghii	Rroxscaffold_4G00316390 Rroxscaffold_6G00409610
rosa_rugosa	Rorug01G0117400 Rorug03G0119000 Rorug03G0119100 Rorug03G0119200
rosa_samantha	Rh1CG133000 Rh3AG170600 Rh3BG196300 Rh3DG178700
rosa_wichuraiana	Rw0G009040 Rw1G011670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	635
Acc36I	ACCTGC	2 cut(s)	548, 609
AccII	CGCG	3 cut(s)	121, 366, 1202
AccIII	TCCGGA	1 cut(s)	1496
AciI	CCGC	7 cut(s)	81, 234, 366, 422, 706, 1200, 1202
AclWI	GGATC	2 cut(s)	117, 1412
AcsI	RAATTY	4 cut(s)	541, 588, 756, 1095
AcuI	CTGAAG	3 cut(s)	1005, 1239, 1487
AcyI	GRCGYC	1 cut(s)	356
AfaI	GTAC	6 cut(s)	103, 247, 458, 1064, 1206, 1403
AfiI	CCNNNNNNNGG	3 cut(s)	22, 365, 466
AflII	CTTAAG	1 cut(s)	1343
AgsI	TTSAA	4 cut(s)	653, 887, 1123, 1577
AjnI	CCWGG	2 cut(s)	207, 846
AloI	GAACNNNNNNTCC	2 cut(s)	333, 365
AluBI	AGCT	7 cut(s)	256, 388, 733, 818, 1342, 1353, 1573
AluI	AGCT	7 cut(s)	256, 388, 733, 818, 1342, 1353, 1573
Alw26I	GTCTC	2 cut(s)	206, 1040
AlwI	GGATC	2 cut(s)	117, 1412
AlwNI	CAGNNNCTG	1 cut(s)	460
Ama87I	CYCGRG	1 cut(s)	53
Aor13HI	TCCGGA	1 cut(s)	1496
AoxI	GGCC	4 cut(s)	109, 168, 231, 971
ApeKI	GCWGC	3 cut(s)	893, 1211, 1265
ApoI	RAATTY	4 cut(s)	541, 588, 756, 1095
Asp700I	GAANNNNTTC	1 cut(s)	1118
AspLEI	GCGC	1 cut(s)	263
AspS9I	GGNCC	3 cut(s)	110, 169, 284
AsuC2I	CCSGG	2 cut(s)	197, 1324
AsuHPI	GGTGA	7 cut(s)	5, 197, 436, 749, 1234, 1516, 1556
AsuII	TTCGAA	1 cut(s)	8
AvaI	CYCGRG	1 cut(s)	53
AvaII	GGWCC	1 cut(s)	284
BaeI	ACNNNNGTAYC	2 cut(s)	1539, 1572
BarI	GAAGNNNNNNTAC	2 cut(s)	18, 50
BauI	CACGAG	2 cut(s)	184, 939
BbsI	GAAGAC	1 cut(s)	434
BbvCI	CCTCAGC	1 cut(s)	1104
BbvI	GCAGC	3 cut(s)	880, 1223, 1252
BccI	CCATC	7 cut(s)	34, 332, 385, 401, 404, 815, 1010
BceAI	ACGGC	1 cut(s)	43
BcgI	CGANNNNNNTGC	2 cut(s)	1362, 1396
BciT130I	CCWGG	2 cut(s)	209, 848
BclI	TGATCA	1 cut(s)	1480
BcnI	CCSGG	2 cut(s)	197, 1324
BcoDI	GTCTC	2 cut(s)	206, 1040
BfrI	CTTAAG	1 cut(s)	1343
BfuAI	ACCTGC	2 cut(s)	548, 609
BglI	GCCNNNNNGGC	1 cut(s)	1370
BisI	GCNGC	4 cut(s)	234, 894, 1212, 1266
BlsI	GCNGC	4 cut(s)	235, 895, 1213, 1267
BmcAI	AGTACT	1 cut(s)	247
Bme1390I	CCNGG	4 cut(s)	197, 209, 848, 1324
Bme18I	GGWCC	1 cut(s)	284
BmeT110I	CYCGRG	1 cut(s)	53
BmgT120I	GGNCC	3 cut(s)	110, 169, 284
BmiI	GGNNCC	3 cut(s)	285, 845, 1363
BmrFI	CCNGG	4 cut(s)	197, 209, 848, 1324
BmrI	ACTGGG	1 cut(s)	321
BmsI	GCATC	5 cut(s)	590, 834, 1015, 1238, 1502
BmuI	ACTGGG	1 cut(s)	321
BoxI	GACNNNNGTC	1 cut(s)	744
BpiI	GAAGAC	1 cut(s)	434
BplI	GAGNNNNNCTC	2 cut(s)	327, 359
BpmI	CTGGAG	2 cut(s)	239, 1380
Bpu10I	CCTNAGC	1 cut(s)	1104
Bpu14I	TTCGAA	1 cut(s)	8
BpuEI	CTTGAG	3 cut(s)	212, 988, 1313
BpuMI	CCSGG	2 cut(s)	197, 1324
BsaHI	GRCGYC	1 cut(s)	356
BsaJI	CCNNGG	9 cut(s)	63, 126, 331, 742, 838, 847, 1200, 1322, 1554
BsaWI	WCCGGW	1 cut(s)	1496
BsaXI	ACNNNNNCTCC	2 cut(s)	507, 537
Bsc4I	CCNNNNNNNGG	3 cut(s)	22, 365, 466
Bse1I	ACTGG	4 cut(s)	316, 1301, 1363, 1443
Bse3DI	GCAATG	1 cut(s)	90
BseAI	TCCGGA	1 cut(s)	1496
BseBI	CCWGG	2 cut(s)	209, 848
BseDI	CCNNGG	9 cut(s)	63, 126, 331, 742, 838, 847, 1200, 1322, 1554
BseGI	GGATG	7 cut(s)	45, 377, 828, 1000, 1021, 1426, 1493
BseLI	CCNNNNNNNGG	3 cut(s)	22, 365, 466
BseMI	GCAATG	1 cut(s)	90
BseMII	CTCAG	4 cut(s)	279, 396, 1035, 1095
BseNI	ACTGG	4 cut(s)	316, 1301, 1363, 1443
BseRI	GAGGAG	1 cut(s)	320
BseXI	GCAGC	3 cut(s)	880, 1223, 1252
BseYI	CCCAGC	1 cut(s)	733
Bsh1236I	CGCG	3 cut(s)	121, 366, 1202
BshFI	GGCC	4 cut(s)	111, 170, 233, 973
BsiHKCI	CYCGRG	1 cut(s)	53
BsiSI	CCGG	3 cut(s)	196, 1324, 1497
BslFI	GGGAC	1 cut(s)	660
BslI	CCNNNNNNNGG	3 cut(s)	22, 365, 466
BsmAI	GTCTC	2 cut(s)	206, 1040
BsmFI	GGGAC	1 cut(s)	660
BsmI	GAATGC	1 cut(s)	4
BsnI	GGCC	4 cut(s)	111, 170, 233, 973
BsoBI	CYCGRG	1 cut(s)	53
Bsp119I	TTCGAA	1 cut(s)	8
Bsp13I	TCCGGA	1 cut(s)	1496
Bsp143I	GATC	7 cut(s)	122, 361, 616, 856, 1417, 1480, 1516
Bsp68I	TCGCGA	1 cut(s)	121
BspACI	CCGC	7 cut(s)	81, 234, 366, 422, 706, 1200, 1202
BspANI	GGCC	4 cut(s)	111, 170, 233, 973
BspCNI	CTCAG	4 cut(s)	280, 395, 1036, 1096
BspEI	TCCGGA	1 cut(s)	1496
BspFNI	CGCG	3 cut(s)	121, 366, 1202
BspLI	GGNNCC	3 cut(s)	285, 845, 1363
BspMI	ACCTGC	2 cut(s)	548, 609
BspPI	GGATC	2 cut(s)	117, 1412
BspQI	GCTCTTC	1 cut(s)	21
BspT104I	TTCGAA	1 cut(s)	8
BspTI	CTTAAG	1 cut(s)	1343
BsrDI	GCAATG	1 cut(s)	90
BsrI	ACTGG	4 cut(s)	316, 1301, 1363, 1443
BssECI	CCNNGG	9 cut(s)	63, 126, 331, 742, 838, 847, 1200, 1322, 1554
BssMI	GATC	7 cut(s)	122, 361, 616, 856, 1417, 1480, 1516
BssNI	GRCGYC	1 cut(s)	356
BssSI	CACGAG	2 cut(s)	184, 939
BssT1I	CCWWGG	1 cut(s)	63
Bst2BI	CACGAG	2 cut(s)	184, 939
Bst2UI	CCWGG	2 cut(s)	209, 848
Bst4CI	ACNGT	3 cut(s)	280, 1087, 1150
Bst6I	CTCTTC	2 cut(s)	21, 1056
BstACI	GRCGYC	1 cut(s)	356
BstAFI	CTTAAG	1 cut(s)	1343
BstBI	TTCGAA	1 cut(s)	8
BstC8I	GCNNGC	4 cut(s)	579, 693, 927, 1413
BstDEI	CTNAG	6 cut(s)	249, 288, 382, 765, 1044, 1104
BstDSI	CCRYGG	4 cut(s)	126, 838, 1200, 1554
BstEII	GGTNACC	1 cut(s)	737
BstF5I	GGATG	7 cut(s)	45, 377, 828, 1000, 1021, 1426, 1493
BstFNI	CGCG	3 cut(s)	121, 366, 1202
BstHHI	GCGC	1 cut(s)	263
BstKTI	GATC	7 cut(s)	125, 364, 619, 859, 1420, 1483, 1519
BstMAI	GTCTC	2 cut(s)	206, 1040
BstMBI	GATC	7 cut(s)	122, 361, 616, 856, 1417, 1480, 1516
BstMWI	GCNNNNNNNGC	4 cut(s)	554, 1199, 1208, 1370
BstNI	CCWGG	2 cut(s)	209, 848
BstNSI	RCATGY	2 cut(s)	581, 695
BstPAI	GACNNNNGTC	1 cut(s)	744
BstPI	GGTNACC	1 cut(s)	737
BstSCI	CCNGG	4 cut(s)	195, 207, 846, 1322
BstUI	CGCG	3 cut(s)	121, 366, 1202
BstV1I	GCAGC	3 cut(s)	880, 1223, 1252
BstV2I	GAAGAC	1 cut(s)	434
BstXI	CCANNNNNNTGG	1 cut(s)	1541
BsuRI	GGCC	4 cut(s)	111, 170, 233, 973
BtgI	CCRYGG	4 cut(s)	126, 838, 1200, 1554
BtsCI	GGATG	7 cut(s)	45, 377, 828, 1000, 1021, 1426, 1493
BtsIMutI	CAGTG	3 cut(s)	276, 1083, 1450
BtuMI	TCGCGA	1 cut(s)	121
BveI	ACCTGC	2 cut(s)	548, 609
Cac8I	GCNNGC	4 cut(s)	579, 693, 927, 1413
CaiI	CAGNNNCTG	1 cut(s)	460
CfoI	GCGC	1 cut(s)	263
Cfr13I	GGNCC	3 cut(s)	110, 169, 284
Cfr42I	CCGCGG	1 cut(s)	1203
CseI	GACGC	2 cut(s)	364, 1182
Csp6I	GTAC	6 cut(s)	102, 246, 457, 1063, 1205, 1402
CviAII	CATG	5 cut(s)	578, 692, 913, 1484, 1514
CviQI	GTAC	6 cut(s)	102, 246, 457, 1063, 1205, 1402
DdeI	CTNAG	6 cut(s)	249, 288, 382, 765, 1044, 1104
DpnI	GATC	7 cut(s)	124, 363, 618, 858, 1419, 1482, 1518
DpnII	GATC	7 cut(s)	122, 361, 616, 856, 1417, 1480, 1516
Eam1104I	CTCTTC	2 cut(s)	21, 1056
EarI	CTCTTC	2 cut(s)	21, 1056
EciI	GGCGGA	1 cut(s)	695
Eco130I	CCWWGG	1 cut(s)	63
Eco47I	GGWCC	1 cut(s)	284
Eco57I	CTGAAG	3 cut(s)	1005, 1239, 1487
Eco88I	CYCGRG	1 cut(s)	53
Eco91I	GGTNACC	1 cut(s)	737
EcoO109I	RGGNCCY	1 cut(s)	284
EcoO65I	GGTNACC	1 cut(s)	737
EcoRI	GAATTC	1 cut(s)	541
EcoRII	CCWGG	2 cut(s)	207, 846
EcoT14I	CCWWGG	1 cut(s)	63
EcoT22I	ATGCAT	2 cut(s)	6, 583
ErhI	CCWWGG	1 cut(s)	63
FaeI	CATG	5 cut(s)	581, 695, 916, 1487, 1517
FalI	AAGNNNNNCTT	2 cut(s)	1337, 1369
FaqI	GGGAC	1 cut(s)	660
FatI	CATG	5 cut(s)	577, 691, 912, 1483, 1513
FauI	CCCGC	1 cut(s)	88
FbaI	TGATCA	1 cut(s)	1480
Fnu4HI	GCNGC	4 cut(s)	234, 894, 1212, 1266
FokI	GGATG	7 cut(s)	52, 364, 815, 1007, 1028, 1413, 1480
Fsp4HI	GCNGC	4 cut(s)	234, 894, 1212, 1266
GlaI	GCGC	1 cut(s)	262
GluI	GCNGC	4 cut(s)	234, 894, 1212, 1266
GsaI	CCCAGC	1 cut(s)	737
GsuI	CTGGAG	2 cut(s)	239, 1380
HaeIII	GGCC	4 cut(s)	111, 170, 233, 973
HapII	CCGG	3 cut(s)	196, 1324, 1497
HgaI	GACGC	2 cut(s)	364, 1182
HhaI	GCGC	1 cut(s)	263
Hin1I	GRCGYC	1 cut(s)	356
Hin1II	CATG	5 cut(s)	581, 695, 916, 1487, 1517
Hin6I	GCGC	1 cut(s)	261
HinP1I	GCGC	1 cut(s)	261
HindIII	AAGCTT	1 cut(s)	254
HpaII	CCGG	3 cut(s)	196, 1324, 1497
HphI	GGTGA	7 cut(s)	5, 197, 436, 749, 1234, 1516, 1556
Hpy166II	GTNNAC	2 cut(s)	465, 570
Hpy188I	TCNGA	7 cut(s)	335, 354, 385, 505, 616, 750, 1467
Hpy8I	GTNNAC	2 cut(s)	465, 570
HpyAV	CCTTC	6 cut(s)	26, 865, 1108, 1219, 1254, 1462
HpyCH4III	ACNGT	3 cut(s)	280, 1087, 1150
HpyF10VI	GCNNNNNNNGC	4 cut(s)	554, 1199, 1208, 1370
HpyF3I	CTNAG	6 cut(s)	249, 288, 382, 765, 1044, 1104
Hsp92I	GRCGYC	1 cut(s)	356
Hsp92II	CATG	5 cut(s)	581, 695, 916, 1487, 1517
HspAI	GCGC	1 cut(s)	261
Kpn2I	TCCGGA	1 cut(s)	1496
Ksp22I	TGATCA	1 cut(s)	1480
KspI	CCGCGG	1 cut(s)	1203
Kzo9I	GATC	7 cut(s)	122, 361, 616, 856, 1417, 1480, 1516
LguI	GCTCTTC	1 cut(s)	21
LmnI	GCTCC	2 cut(s)	1350, 1361
Lsp1109I	GCAGC	3 cut(s)	880, 1223, 1252
LweI	GCATC	5 cut(s)	590, 834, 1015, 1238, 1502
MaeIII	GTNAC	4 cut(s)	737, 908, 934, 1509
MalI	GATC	7 cut(s)	124, 363, 618, 858, 1419, 1482, 1518
MboI	GATC	7 cut(s)	122, 361, 616, 856, 1417, 1480, 1516
MboII	GAAGA	9 cut(s)	11, 38, 103, 439, 530, 710, 1025, 1043, 1245
MluCI	AATT	5 cut(s)	541, 588, 756, 1029, 1095
MlyI	GAGTC	4 cut(s)	208, 336, 1042, 1466
MmeI	TCCRAC	3 cut(s)	252, 474, 1141
Mph1103I	ATGCAT	2 cut(s)	6, 583
MroI	TCCGGA	1 cut(s)	1496
MroXI	GAANNNNTTC	1 cut(s)	1118
MseI	TTAA	6 cut(s)	240, 726, 788, 1140, 1218, 1344
MslI	CAYNNNNRTG	1 cut(s)	798
MspA1I	CMGCKG	2 cut(s)	236, 1202
MspCI	CTTAAG	1 cut(s)	1343
MspI	CCGG	3 cut(s)	196, 1324, 1497
MspR9I	CCNGG	4 cut(s)	197, 209, 848, 1324
Mva1269I	GAATGC	1 cut(s)	4
MvaI	CCWGG	2 cut(s)	209, 848
MvnI	CGCG	3 cut(s)	121, 366, 1202
MwoI	GCNNNNNNNGC	4 cut(s)	554, 1199, 1208, 1370
NciI	CCSGG	2 cut(s)	197, 1324
NdeII	GATC	7 cut(s)	122, 361, 616, 856, 1417, 1480, 1516
NlaIII	CATG	5 cut(s)	581, 695, 916, 1487, 1517
NlaIV	GGNNCC	3 cut(s)	285, 845, 1363
NmeAIII	GCCGAG	1 cut(s)	87
NmuCI	GTSAC	2 cut(s)	737, 1509
NruI	TCGCGA	1 cut(s)	121
NsiI	ATGCAT	2 cut(s)	6, 583
NspI	RCATGY	2 cut(s)	581, 695
NspV	TTCGAA	1 cut(s)	8
PaeI	GCATGC	2 cut(s)	581, 695
PaeR7I	CTCGAG	1 cut(s)	53
PciSI	GCTCTTC	1 cut(s)	21
PcsI	WCGNNNNNNNCGW	2 cut(s)	110, 531
PctI	GAATGC	1 cut(s)	4
PdmI	GAANNNNTTC	1 cut(s)	1118
PfeI	GAWTC	8 cut(s)	10, 49, 174, 223, 316, 805, 1184, 1436
PkrI	GCNGC	4 cut(s)	235, 895, 1213, 1267
PleI	GAGTC	4 cut(s)	208, 336, 1042, 1465
PpsI	GAGTC	4 cut(s)	208, 336, 1042, 1465
PpuMI	RGGWCCY	1 cut(s)	284
PshAI	GACNNNNGTC	1 cut(s)	744
PsiI	TTATAA	1 cut(s)	635
Psp5II	RGGWCCY	1 cut(s)	284
Psp6I	CCWGG	2 cut(s)	207, 846
PspEI	GGTNACC	1 cut(s)	737
PspFI	CCCAGC	1 cut(s)	733
PspGI	CCWGG	2 cut(s)	207, 846
PspN4I	GGNNCC	3 cut(s)	285, 845, 1363
PspPI	GGNCC	3 cut(s)	110, 169, 284
PspPPI	RGGWCCY	1 cut(s)	284
PstNI	CAGNNNCTG	1 cut(s)	460
RruI	TCGCGA	1 cut(s)	121
RsaI	GTAC	6 cut(s)	103, 247, 458, 1064, 1206, 1403
RsaNI	GTAC	6 cut(s)	102, 246, 457, 1063, 1205, 1402
RseI	CAYNNNNRTG	1 cut(s)	798
SacII	CCGCGG	1 cut(s)	1203
SapI	GCTCTTC	1 cut(s)	21
SaqAI	TTAA	6 cut(s)	240, 726, 788, 1140, 1218, 1344
SatI	GCNGC	4 cut(s)	234, 894, 1212, 1266
Sau3AI	GATC	7 cut(s)	122, 361, 616, 856, 1417, 1480, 1516
Sau96I	GGNCC	3 cut(s)	110, 169, 284
ScaI	AGTACT	1 cut(s)	247
SchI	GAGTC	4 cut(s)	208, 336, 1042, 1466
ScrFI	CCNGG	4 cut(s)	197, 209, 848, 1324
SfaNI	GCATC	5 cut(s)	590, 834, 1015, 1238, 1502
Sfr274I	CTCGAG	1 cut(s)	53
Sfr303I	CCGCGG	1 cut(s)	1203
SfuI	TTCGAA	1 cut(s)	8
SgrBI	CCGCGG	1 cut(s)	1203
SinI	GGWCC	1 cut(s)	284
SlaI	CTCGAG	1 cut(s)	53
SmiMI	CAYNNNNRTG	1 cut(s)	798
SmlI	CTYRAG	5 cut(s)	53, 227, 967, 1328, 1343
SmoI	CTYRAG	5 cut(s)	53, 227, 967, 1328, 1343
SphI	GCATGC	2 cut(s)	581, 695
Sse9I	AATT	5 cut(s)	541, 588, 756, 1029, 1095
SsiI	CCGC	7 cut(s)	81, 234, 366, 422, 706, 1200, 1202
StyD4I	CCNGG	4 cut(s)	195, 207, 846, 1322
StyI	CCWWGG	1 cut(s)	63
TaaI	ACNGT	3 cut(s)	280, 1087, 1150
TaqII	GACCGA	1 cut(s)	734
TasI	AATT	5 cut(s)	541, 588, 756, 1029, 1095
TatI	WGTACW	1 cut(s)	245
TauI	GCSGC	1 cut(s)	236
TfiI	GAWTC	8 cut(s)	10, 49, 174, 223, 316, 805, 1184, 1436
Tru1I	TTAA	6 cut(s)	240, 726, 788, 1140, 1218, 1344
Tru9I	TTAA	6 cut(s)	240, 726, 788, 1140, 1218, 1344
TscAI	CASTG	3 cut(s)	283, 1090, 1450
TseFI	GTSAC	2 cut(s)	737, 1509
TseI	GCWGC	3 cut(s)	893, 1211, 1265
Tsp45I	GTSAC	2 cut(s)	737, 1509
TspDTI	ATGAA	1 cut(s)	552
TspGWI	ACGGA	5 cut(s)	52, 115, 523, 1007, 1543
TspRI	CASTG	3 cut(s)	283, 1090, 1450
Vha464I	CTTAAG	1 cut(s)	1343
VpaK11BI	GGWCC	1 cut(s)	284
XapI	RAATTY	4 cut(s)	541, 588, 756, 1095
XceI	RCATGY	2 cut(s)	581, 695
XhoI	CTCGAG	1 cut(s)	53
XmnI	GAANNNNTTC	1 cut(s)	1118
ZrmI	AGTACT	1 cut(s)	247
Zsp2I	ATGCAT	2 cut(s)	6, 583

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.