AT3G52990

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001999
Physical Location & Seq
Forward (+)
19648711 .. 19652515
3805 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G52990.1

Sequence Viewer

Length: 1584 bp
ATGCATTCCAGTCATCTTCTTCTCGAGGAGCCGATCAGGATGGCTTCAATCCTCGAGCCTTCCAAATCTAGTTTCTTCCCGGCATTGACTAAGATCGTCGGAACTCTAGGTCCTAAATCCCGATCCGTCGAGGCTCTCTCCGGCTGTCTCAAAGCCGGCATGTCTGTGGCTCGATTTGATTTCTCGTGGGGAGATGCTGATTATCACCAGGAGACACTTGATAATTTGAAAGTTGCTGTGAGGAGCACTAAGAAGCTTTGTGCTGTTATGCTTGATACTGTTGGACCTGAGCTACAAGTTATTAACAAATCTGAGAAAGCTATTACTTTGAAAGCTGATGGCCTTGTAACTTTGACACCGAATCAAGATCAAGAAGCTTCTTCTGAAGTTCTTCCCATTAATTTCAATGGACTTGCCAAGGCAGTGAAGAAAGGAGACACCATCTTCGTTGGGCAATACCTATTCACTGGTAGTGAAACAACTTCAGTTTGGCTCGAGGTTGATGAAGTTAAAGGAGATGATGTCATTTGCCTTTCAAGGAATGCTGCTACTCTGGCTGGTTCTTTGTTCACTTTACACTCCTCTCAAGTTCACATTGATCTCCCAACGCTTACAGAGAAGGATAAGGAGGTTATAAGCACATGGGGAGTTCAAAATAAAATCGATTTTCTCTCATTGTCTTATTGTCGTCATGCTGAGGATGTTCGCCAGACCCGTGAAATGCTTAAAAAGTTGGGCGACCTCTCTCAAACACAAATATTTGCTAAGATTGAGAACGTAGAGGGACTAACCCACTTCGATGAGATTCTACAAGAAGCTGATGGCATTATTCTTTCTCGTGGGAATTTGGGTATAGATTTACCCCCGGAAAAGGTGTTTTTGTTTCAAAAGGCTGCTCTTTACAAGTGCAACATGGCTGGAAAGCCAGCTGTTCTTACCCGTGTTGTTGACAGTATGACTGACAACTTGCGACCAACTCGTGCTGAGGCCACGGATGTTGCTAATGCTGTTTTAGATGGAAGTGATGCAATTCTTCTTGGTGCTGAGACCCTTCGTGGATTGTACCCTGTTGAGACAATATCAACTGTTGGTAGGATCTGTGCTGAGGCAGAAAAGGTTTTCAATCAAGATTTGTACTTTAAGAAGACTGTCAAGTATGTTGGAGAACCAATGACTCACTTGGAATCGATTGCTTCTTCAGCTGTACGGGCAGCCATTAAGGTTAAGGCATCCGTAATTATATGCTTCACCTCTTCTGGAAGAGCAGCCAGATTGATTGCCAAATACAGGCCAACGATGCCGGTTATTTCTGTTGTCATTCCCCGGGTTAAGACAAATCAACTGAAATGGAGCTTTAGTGGAGCTTTTGAGGCGAGGCAGTCACTTATTGTCAGAGGCCTCTTCCCTATGCTTGCTGACCCTCGTCACCCTGCGGAATCTACAAGTGCGACAAACGAGTCAGTCCTGAAGGTTGCTCTAGACCATGGGAAGCATGCTGGAGTAATCAAGTCGCATGACAGAGTAGTGGTGTGTCAGAAAGTTGGTGATGCATCTGTGGTTAAGATCATTGAGCTTGAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

527

Amino Acids

57.49

Weight (kDa)

6.66

Isoelectric Point (pI)

28.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 30 - 360 6.5e-89 Pyruvate kinase, barrel domain
PK_C PF02887 395 - 515 1.5e-22 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 635
AasI GACNNNNNNGTC 1 cut(s) 1456
AciI CCGC 1 cut(s) 1433
AclWI GGATC 2 cut(s) 117, 1103
AcsI RAATTY 1 cut(s) 844
AcuI CTGAAG 4 cut(s) 405, 468, 1182, 1487
AfaI GTAC 3 cut(s) 1064, 1136, 1206
AfiI CCNNNNNNNGG 2 cut(s) 871, 1287
AgsI TTSAA 8 cut(s) 48, 229, 331, 406, 537, 653, 887, 1123
AjnI CCWGG 1 cut(s) 207
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AloI GAACNNNNNNTCC 2 cut(s) 94, 126
Alw21I GWGCWC 1 cut(s) 248
Alw26I GTCTC 5 cut(s) 152, 206, 429, 1040, 1067
AlwI GGATC 2 cut(s) 117, 1103
Ama87I CYCGRG 4 cut(s) 23, 53, 494, 1323
AoxI GGCC 4 cut(s) 340, 987, 1289, 1396
ApeKI GCWGC 4 cut(s) 545, 893, 1211, 1265
ApoI RAATTY 1 cut(s) 844
ArsI GACNNNNNNTTYG 2 cut(s) 428, 460
AseI ATTAAT 1 cut(s) 399
Asp700I GAANNNNTTC 1 cut(s) 390
AspS9I GGNCC 2 cut(s) 110, 284
AsuC2I CCSGG 4 cut(s) 80, 866, 1324, 1325
AsuHPI GGTGA 4 cut(s) 197, 1240, 1418, 1556
AvaI CYCGRG 4 cut(s) 23, 53, 494, 1323
AvaII GGWCC 2 cut(s) 110, 284
BauI CACGAG 3 cut(s) 184, 837, 978
BbsI GAAGAC 1 cut(s) 1151
Bbv12I GWGCWC 1 cut(s) 248
BbvCI CCTCAGC 3 cut(s) 696, 984, 1104
BbvI GCAGC 4 cut(s) 532, 880, 1223, 1277
BccI CCATC 5 cut(s) 34, 332, 449, 815, 1010
BciT130I CCWGG 1 cut(s) 209
BcnI CCSGG 4 cut(s) 80, 866, 1324, 1325
BcoDI GTCTC 5 cut(s) 152, 206, 429, 1040, 1067
BfaI CTAG 3 cut(s) 69, 107, 1478
BisI GCNGC 4 cut(s) 546, 894, 1212, 1266
BlsI GCNGC 4 cut(s) 547, 895, 1213, 1267
Bme1390I CCNGG 5 cut(s) 80, 209, 866, 1324, 1325
Bme18I GGWCC 2 cut(s) 110, 284
BmeT110I CYCGRG 4 cut(s) 23, 53, 494, 1323
BmgT120I GGNCC 2 cut(s) 110, 284
BmiI GGNNCC 1 cut(s) 30
BmrFI CCNGG 5 cut(s) 80, 209, 866, 1324, 1325
BmsI GCATC 6 cut(s) 184, 1015, 1238, 1287, 1537, 1559
BoxI GACNNNNGTC 1 cut(s) 1422
BpiI GAAGAC 1 cut(s) 1151
BplI GAGNNNNNCTC 2 cut(s) 122, 154
BpmI CTGGAG 1 cut(s) 1518
Bpu10I CCTNAGC 4 cut(s) 288, 696, 984, 1104
BpuEI CTTGAG 1 cut(s) 570
BpuMI CCSGG 4 cut(s) 80, 866, 1324, 1325
Bsa29I ATCGAT 2 cut(s) 663, 1187
BsaI GGTCTC 1 cut(s) 1040
BsaJI CCNNGG 6 cut(s) 417, 864, 990, 1322, 1323, 1483
BsaXI ACNNNNNCTCC 4 cut(s) 507, 537, 1342, 1372
Bsc4I CCNNNNNNNGG 2 cut(s) 871, 1287
Bse118I RCCGGY 2 cut(s) 155, 1300
Bse1I ACTGG 2 cut(s) 9, 472
BseBI CCWGG 1 cut(s) 209
BseCI ATCGAT 2 cut(s) 663, 1187
BseDI CCNNGG 6 cut(s) 417, 864, 990, 1322, 1323, 1483
BseGI GGATG 4 cut(s) 45, 706, 1000, 1229
BseLI CCNNNNNNNGG 2 cut(s) 871, 1287
BseMII CTCAG 6 cut(s) 279, 303, 687, 975, 1035, 1095
BseNI ACTGG 2 cut(s) 9, 472
BseRI GAGGAG 3 cut(s) 41, 256, 571
BseXI GCAGC 4 cut(s) 532, 880, 1223, 1277
BshFI GGCC 4 cut(s) 342, 989, 1291, 1398
BshVI ATCGAT 2 cut(s) 663, 1187
BsiHKAI GWGCWC 1 cut(s) 248
BsiHKCI CYCGRG 4 cut(s) 23, 53, 494, 1323
BsiSI CCGG 6 cut(s) 80, 141, 156, 866, 1301, 1324
BslFI GGGAC 1 cut(s) 798
BslI CCNNNNNNNGG 2 cut(s) 871, 1287
BsmAI GTCTC 5 cut(s) 152, 206, 429, 1040, 1067
BsmFI GGGAC 1 cut(s) 798
BsmI GAATGC 2 cut(s) 4, 547
BsnI GGCC 4 cut(s) 342, 989, 1291, 1398
Bso31I GGTCTC 1 cut(s) 1040
BsoBI CYCGRG 4 cut(s) 23, 53, 494, 1323
Bsp1286I GDGCHC 1 cut(s) 248
Bsp143I GATC 7 cut(s) 33, 93, 122, 367, 598, 1095, 1563
Bsp19I CCATGG 1 cut(s) 1483
BspACI CCGC 1 cut(s) 1433
BspANI GGCC 4 cut(s) 342, 989, 1291, 1398
BspCNI CTCAG 6 cut(s) 280, 304, 688, 976, 1036, 1096
BspDI ATCGAT 2 cut(s) 663, 1187
BspLI GGNNCC 1 cut(s) 30
BspPI GGATC 2 cut(s) 117, 1103
BspQI GCTCTTC 1 cut(s) 1255
BspTNI GGTCTC 1 cut(s) 1040
BsrFI RCCGGY 2 cut(s) 155, 1300
BsrI ACTGG 2 cut(s) 9, 472
BssAI RCCGGY 2 cut(s) 155, 1300
BssECI CCNNGG 6 cut(s) 417, 864, 990, 1322, 1323, 1483
BssMI GATC 7 cut(s) 33, 93, 122, 367, 598, 1095, 1563
BssSI CACGAG 3 cut(s) 184, 837, 978
BssT1I CCWWGG 2 cut(s) 417, 1483
Bst2BI CACGAG 3 cut(s) 184, 837, 978
Bst2UI CCWGG 1 cut(s) 209
Bst4CI ACNGT 4 cut(s) 280, 953, 1087, 1150
Bst6I CTCTTC 3 cut(s) 1255, 1258, 1406
BstC8I GCNNGC 4 cut(s) 157, 927, 1413, 1494
BstDEI CTNAG 9 cut(s) 90, 249, 288, 312, 696, 765, 984, 1044, 1104
BstDSI CCRYGG 2 cut(s) 990, 1483
BstF5I GGATG 4 cut(s) 45, 706, 1000, 1229
BstKTI GATC 7 cut(s) 36, 96, 125, 370, 601, 1098, 1566
BstMAI GTCTC 5 cut(s) 152, 206, 429, 1040, 1067
BstMBI GATC 7 cut(s) 33, 93, 122, 367, 598, 1095, 1563
BstMWI GCNNNNNNNGC 5 cut(s) 554, 1199, 1208, 1297, 1370
BstNI CCWGG 1 cut(s) 209
BstNSI RCATGY 2 cut(s) 163, 1496
BstPAI GACNNNNGTC 1 cut(s) 1422
BstSCI CCNGG 5 cut(s) 78, 207, 864, 1322, 1323
BstV1I GCAGC 4 cut(s) 532, 880, 1223, 1277
BstV2I GAAGAC 1 cut(s) 1151
BstX2I RGATCY 1 cut(s) 1095
BstYI RGATCY 1 cut(s) 1095
Bsu15I ATCGAT 2 cut(s) 663, 1187
BsuRI GGCC 4 cut(s) 342, 989, 1291, 1398
BsuTUI ATCGAT 2 cut(s) 663, 1187
BtgI CCRYGG 2 cut(s) 990, 1483
BtsCI GGATG 4 cut(s) 45, 706, 1000, 1229
BtsI GCAGTG 1 cut(s) 429
BtsIMutI CAGTG 2 cut(s) 429, 465
Cac8I GCNNGC 4 cut(s) 157, 927, 1413, 1494
Cfr10I RCCGGY 2 cut(s) 155, 1300
Cfr13I GGNCC 2 cut(s) 110, 284
Cfr9I CCCGGG 1 cut(s) 1323
ClaI ATCGAT 2 cut(s) 663, 1187
Csp6I GTAC 3 cut(s) 1063, 1135, 1205
CspCI CAANNNNNGTGG 2 cut(s) 979, 1014
CviAII CATG 7 cut(s) 160, 642, 692, 913, 1484, 1493, 1514
CviQI GTAC 3 cut(s) 1063, 1135, 1205
DdeI CTNAG 9 cut(s) 90, 249, 288, 312, 696, 765, 984, 1044, 1104
DpnI GATC 7 cut(s) 35, 95, 124, 369, 600, 1097, 1565
DpnII GATC 7 cut(s) 33, 93, 122, 367, 598, 1095, 1563
DrdI GACNNNNNNGTC 1 cut(s) 1456
DseDI GACNNNNNNGTC 1 cut(s) 1456
Eam1104I CTCTTC 3 cut(s) 1255, 1258, 1406
EarI CTCTTC 3 cut(s) 1255, 1258, 1406
Eco130I CCWWGG 2 cut(s) 417, 1483
Eco147I AGGCCT 1 cut(s) 1398
Eco31I GGTCTC 1 cut(s) 1040
Eco47I GGWCC 2 cut(s) 110, 284
Eco57I CTGAAG 4 cut(s) 405, 468, 1182, 1487
Eco88I CYCGRG 4 cut(s) 23, 53, 494, 1323
EcoO109I RGGNCCY 1 cut(s) 110
EcoRII CCWGG 1 cut(s) 207
EcoT14I CCWWGG 2 cut(s) 417, 1483
EcoT22I ATGCAT 2 cut(s) 6, 1552
ErhI CCWWGG 2 cut(s) 417, 1483
FaeI CATG 7 cut(s) 163, 645, 695, 916, 1487, 1496, 1517
FaqI GGGAC 1 cut(s) 798
FatI CATG 7 cut(s) 159, 641, 691, 912, 1483, 1492, 1513
Fnu4HI GCNGC 4 cut(s) 546, 894, 1212, 1266
FokI GGATG 4 cut(s) 52, 713, 1007, 1216
Fsp4HI GCNGC 4 cut(s) 546, 894, 1212, 1266
FspBI CTAG 3 cut(s) 69, 107, 1478
GluI GCNGC 4 cut(s) 546, 894, 1212, 1266
GsuI CTGGAG 1 cut(s) 1518
HaeIII GGCC 4 cut(s) 342, 989, 1291, 1398
HapII CCGG 6 cut(s) 80, 141, 156, 866, 1301, 1324
Hin1II CATG 7 cut(s) 163, 645, 695, 916, 1487, 1496, 1517
HincII GTYRAC 1 cut(s) 949
HindII GTYRAC 1 cut(s) 949
HindIII AAGCTT 2 cut(s) 254, 375
HinfI GANTC 6 cut(s) 361, 805, 1174, 1184, 1436, 1457
HpaII CCGG 6 cut(s) 80, 141, 156, 866, 1301, 1324
HphI GGTGA 4 cut(s) 197, 1240, 1418, 1556
Hpy166II GTNNAC 3 cut(s) 570, 592, 949
Hpy188I TCNGA 5 cut(s) 101, 313, 385, 1394, 1536
Hpy188III TCNNGA 9 cut(s) 23, 37, 120, 365, 371, 1127, 1257, 1465, 1478
Hpy8I GTNNAC 3 cut(s) 570, 592, 949
Hpy99I CGWCG 2 cut(s) 101, 131
HpyAV CCTTC 4 cut(s) 69, 613, 1061, 1462
HpyCH4III ACNGT 4 cut(s) 280, 953, 1087, 1150
HpyCH4IV ACGT 1 cut(s) 777
HpyCH4V TGCA 4 cut(s) 4, 909, 1028, 1550
HpyF10VI GCNNNNNNNGC 5 cut(s) 554, 1199, 1208, 1297, 1370
HpyF3I CTNAG 9 cut(s) 90, 249, 288, 312, 696, 765, 984, 1044, 1104
HpySE526I ACGT 1 cut(s) 777
Hsp92II CATG 7 cut(s) 163, 645, 695, 916, 1487, 1496, 1517
KroI GCCGGC 1 cut(s) 155
KroNI GCCGGC 1 cut(s) 157
Kzo9I GATC 7 cut(s) 33, 93, 122, 367, 598, 1095, 1563
LguI GCTCTTC 1 cut(s) 1255
LmnI GCTCC 4 cut(s) 28, 243, 1350, 1361
Lsp1109I GCAGC 4 cut(s) 532, 880, 1223, 1277
LweI GCATC 6 cut(s) 184, 1015, 1238, 1287, 1537, 1559
MaeI CTAG 3 cut(s) 69, 107, 1478
MaeII ACGT 1 cut(s) 777
MaeIII GTNAC 3 cut(s) 346, 1380, 1424
MalI GATC 7 cut(s) 35, 95, 124, 369, 600, 1097, 1565
MboI GATC 7 cut(s) 33, 93, 122, 367, 598, 1095, 1563
MflI RGATCY 1 cut(s) 1095
MhlI GDGCHC 1 cut(s) 248
MluCI AATT 5 cut(s) 223, 400, 844, 1029, 1236
MlyI GAGTC 2 cut(s) 1168, 1466
MmeI TCCRAC 3 cut(s) 79, 262, 1141
Mph1103I ATGCAT 2 cut(s) 6, 1552
MroNI GCCGGC 1 cut(s) 155
MroXI GAANNNNTTC 1 cut(s) 390
MslI CAYNNNNRTG 2 cut(s) 164, 798
MspA1I CMGCKG 2 cut(s) 929, 1202
MspI CCGG 6 cut(s) 80, 141, 156, 866, 1301, 1324
MspR9I CCNGG 5 cut(s) 80, 209, 866, 1324, 1325
Mva1269I GAATGC 2 cut(s) 4, 547
MvaI CCWGG 1 cut(s) 209
MwoI GCNNNNNNNGC 5 cut(s) 554, 1199, 1208, 1297, 1370
NaeI GCCGGC 1 cut(s) 157
NciI CCSGG 4 cut(s) 80, 866, 1324, 1325
NcoI CCATGG 1 cut(s) 1483
NdeII GATC 7 cut(s) 33, 93, 122, 367, 598, 1095, 1563
NgoMIV GCCGGC 1 cut(s) 155
NlaIII CATG 7 cut(s) 163, 645, 695, 916, 1487, 1496, 1517
NlaIV GGNNCC 1 cut(s) 30
NmuCI GTSAC 2 cut(s) 1380, 1424
NsiI ATGCAT 2 cut(s) 6, 1552
NspI RCATGY 2 cut(s) 163, 1496
PaeI GCATGC 1 cut(s) 1496
PaeR7I CTCGAG 3 cut(s) 23, 53, 494
PceI AGGCCT 1 cut(s) 1398
PciSI GCTCTTC 1 cut(s) 1255
PcsI WCGNNNNNNNCGW 1 cut(s) 712
PctI GAATGC 2 cut(s) 4, 547
PdiI GCCGGC 1 cut(s) 157
PdmI GAANNNNTTC 1 cut(s) 390
PfeI GAWTC 4 cut(s) 361, 805, 1184, 1436
PkrI GCNGC 4 cut(s) 547, 895, 1213, 1267
PleI GAGTC 2 cut(s) 1168, 1465
PpsI GAGTC 2 cut(s) 1168, 1465
PpuMI RGGWCCY 1 cut(s) 110
PshAI GACNNNNGTC 1 cut(s) 1422
PshBI ATTAAT 1 cut(s) 399
PsiI TTATAA 1 cut(s) 635
Psp5II RGGWCCY 1 cut(s) 110
Psp6I CCWGG 1 cut(s) 207
PspGI CCWGG 1 cut(s) 207
PspN4I GGNNCC 1 cut(s) 30
PspPI GGNCC 2 cut(s) 110, 284
PspPPI RGGWCCY 1 cut(s) 110
PspXI VCTCGAGB 2 cut(s) 53, 494
PsuI RGATCY 1 cut(s) 1095
PvuII CAGCTG 2 cut(s) 929, 1202
RsaI GTAC 3 cut(s) 1064, 1136, 1206
RsaNI GTAC 3 cut(s) 1063, 1135, 1205
RseI CAYNNNNRTG 2 cut(s) 164, 798
SapI GCTCTTC 1 cut(s) 1255
SatI GCNGC 4 cut(s) 546, 894, 1212, 1266
Sau3AI GATC 7 cut(s) 33, 93, 122, 367, 598, 1095, 1563
Sau96I GGNCC 2 cut(s) 110, 284
SchI GAGTC 2 cut(s) 1168, 1466
ScrFI CCNGG 5 cut(s) 80, 209, 866, 1324, 1325
SduI GDGCHC 1 cut(s) 248
SfaNI GCATC 6 cut(s) 184, 1015, 1238, 1287, 1537, 1559
Sfr274I CTCGAG 3 cut(s) 23, 53, 494
SinI GGWCC 2 cut(s) 110, 284
SlaI CTCGAG 3 cut(s) 23, 53, 494
SmaI CCCGGG 1 cut(s) 1325
SmiMI CAYNNNNRTG 2 cut(s) 164, 798
SmlI CTYRAG 5 cut(s) 23, 53, 494, 585, 1574
SmoI CTYRAG 5 cut(s) 23, 53, 494, 585, 1574
SphI GCATGC 1 cut(s) 1496
Sse9I AATT 5 cut(s) 223, 400, 844, 1029, 1236
SseBI AGGCCT 1 cut(s) 1398
SsiI CCGC 1 cut(s) 1433
SspI AATATT 1 cut(s) 759
SspMI CTAG 3 cut(s) 69, 107, 1478
StuI AGGCCT 1 cut(s) 1398
StyD4I CCNGG 5 cut(s) 78, 207, 864, 1322, 1323
StyI CCWWGG 2 cut(s) 417, 1483
TaaI ACNGT 4 cut(s) 280, 953, 1087, 1150
TaiI ACGT 1 cut(s) 780
TaqI TCGA 8 cut(s) 24, 54, 129, 172, 495, 663, 798, 1187
TasI AATT 5 cut(s) 223, 400, 844, 1029, 1236
TatI WGTACW 1 cut(s) 1134
TfiI GAWTC 4 cut(s) 361, 805, 1184, 1436
TscAI CASTG 2 cut(s) 429, 472
TseFI GTSAC 2 cut(s) 1380, 1424
TseI GCWGC 4 cut(s) 545, 893, 1211, 1265
Tsp45I GTSAC 2 cut(s) 1380, 1424
TspDTI ATGAA 1 cut(s) 519
TspGWI ACGGA 3 cut(s) 115, 1007, 1222
TspMI CCCGGG 1 cut(s) 1323
TspRI CASTG 2 cut(s) 429, 472
VpaK11BI GGWCC 2 cut(s) 110, 284
VspI ATTAAT 1 cut(s) 399
XapI RAATTY 1 cut(s) 844
XbaI TCTAGA 1 cut(s) 1477
XceI RCATGY 2 cut(s) 163, 1496
XhoI CTCGAG 3 cut(s) 23, 53, 494
XmaI CCCGGG 1 cut(s) 1323
XmnI GAANNNNTTC 1 cut(s) 390
XspI CTAG 3 cut(s) 69, 107, 1478
Zsp2I ATGCAT 2 cut(s) 6, 1552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.