Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0001999

Physical Location & Seq

Genomic Coordinates Forward (+)

6915190 .. 6932986

Gene Structure 17797 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1073000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1584 bp

ATGCATTCGACTCACTTGTTTCTGGAAGAGCCGATCAGGATGGCTTCGATTCTCGAGCCTTCCAAGCCTACATTCTTTCCAGCAATGACAAGGATCGTGGGAACGCTTGGTCCAAAATCACGATCGGTTGAGACAATCTCCGGTTGCTTGAAGGCAGGAATGTCTGTGGCACGGTTTGATTTTTCATGGGGCGATGCTCAGTTTCACCAAGAGACATTGGGAAACCTGAAGGTTGCAATGAAGAGTACCAATAAACTGTGTGCAGTAATGCTTGACACAGTGGGTCCAGAGTTGCAGGTAGTGAATAGAAGTGAGCATCCCATATCTCTTCAGGAAGATACCCTGGTTGTCCTAACACCTGATCAAGACAAAGAGGCCACCTCAAATCTACTACCCATAAATTTTAGTGGCCTGGCAAAGGCAGTGAAGAAGGGAGATACAATTTTTATTGGTCAATACCTCTTTACGGGAAGTGAAACTACTTCTGTGTGGCTGGAGGTCACTGAGGTAAATGGTGAAGACGTGGTTTGTCTCATAAAGAATTCTGCTACCTTGTCAGGGTCATTGTATACTTTGCATGTCTCTCAGATTCGTATTGATCTGCCTACACTTAACGATAAAGATAAGGAGGTCATAAGCACTTGGGGTGTTCGTAACACCATCGACTTCCTCTCATTATCATATACGCGTCATGCTGAAGATATTCGCCACGCCCGTGAGTTTCTTTCAAAGTTAGGCGACCTCAACCAAACTCAGATTTTTGCAAAAATTGAAAATGTAGAGGGTTTAAATCATTTTGATGAAATTTTGCAAGAAGCTGATGGTATTATCCTCTCTCGTGGAAATCTGGGGATAGATCTCCCACCAGAGAAGGTATTTTTATTTCAAAAGGCTGCTGTTTACAAGTGCAACATGGCTGGAAAGCCAGCAGTGGTGACTCGGGTTGTGGACAGTATGACCGACAACCTTAGACCTACTCGTGCTGAAGCAACTGATGTTGCCAACGCTGTACTGGATGGAAGTGATGCAATTCTTCTAGGTGCAGAGACCCTGCGCGGTTTGTACCCTGTTGAGACGATCTCCATAGTTGGAAAGATTTGCTCTGAGGCGGAGAAAGTTTTCAATCAAGATTTGTATTTTAAGAGGGCTGTCAAATATGTTGGAGAACCAATGACCCACTTGGAATCTATTGCTTCCTCTGCGGTACGTGCAGCCATCAAGGTGAAGGCCTCTGTAATTATTTGCTTCACTACATCTGGAAGAGCTGCAAGGTTGATTGCAAAGTACAGACCAACAATGCCTGTGCTTTCTGTTGTCATACCTCGGGTCAAGACAAATCAACTCCGATGGACATTTTCTGGTGCTTTTGAGGCAAGGCAATCACTCATTGTTAGAGGCCTTTTTCCCATGCTAGCAGATCCTCGACATCCAGCTGAAACTACCGGTGCAACAAATGAGTCCATTCTTAAAATCGCTTTGGACCATGGCAAGGCCATTGGTGTCATAAAATCACATGATCGTGTGGTCATTTGCCAAAAAATTGGCGATACTTCTGTGATCAAGATCATGGAGCTTGAAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230

Pfam Domains

Protein Families

PK PK_C

Protein Analysis

528

Amino Acids

57.88

Weight (kDa)

6.49

Isoelectric Point (pI)

25.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK	PF00224	30 - 360	7.1e-87	Pyruvate kinase, barrel domain
PK_C	PF02887	395 - 522	1.1e-21	Pyruvate kinase, alpha/beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001999)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36580 AT3G52990 AT3G52990
fragaria_vesca	FvH4_6g17480 FvH4_6g17480 FvH4_6g17480 FvH4_6g17480 FvH4_7g06490 FvH4_7g06490 FvH4_7g06490 FvH4_7g06490 FvH4_7g06490
malus_domestica	MD02G1244000.v1.1 MD07G1072900.v1.1 MD07G1073000.v1.1 MD12G1096900.v1.1 MD14G1094200.v1.1
prunus_persica	Prupe.2G091200_v2.0.a1 Prupe.2G091200_v2.0.a1 Prupe.2G091200_v2.0.a1 Prupe.7G120700_v2.0.a1 Prupe.7G120700_v2.0.a1
pyrus_communis	pycom07g05600 pycom07g05630 pycom12g09800 pycom14g09200
rosa_chinensis	RchiOBHm_Chr1g0334861 RchiOBHm_Chr3g0471721
rosa_laevigata	RLG00000024139 RLG00000029414
rosa_multiflora	Rmu_sc0000574.1_g000046 Rmu_sc0005631.1_g000026 Rmu_sc0026537.1_g000003
rosa_roxburghii	Rroxscaffold_4G00316390 Rroxscaffold_6G00409610
rosa_rugosa	Rorug01G0117400 Rorug03G0119000 Rorug03G0119100 Rorug03G0119200
rosa_samantha	Rh1CG133000 Rh3AG170600 Rh3BG196300 Rh3DG178700
rosa_wichuraiana	Rw0G009040 Rw1G011670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	286
AccI	GTMKAC	1 cut(s)	569
AccII	CGCG	2 cut(s)	688, 1056
AciI	CCGC	3 cut(s)	1056, 1109, 1202
AclWI	GGATC	2 cut(s)	101, 1412
AcsI	RAATTY	3 cut(s)	400, 541, 804
AcuI	CTGAAG	4 cut(s)	248, 314, 717, 1005
AfaI	GTAC	5 cut(s)	247, 1011, 1064, 1206, 1286
AfiI	CCNNNNNNNGG	4 cut(s)	418, 466, 558, 1489
AflIII	ACRYGT	1 cut(s)	686
AgeI	ACCGGT	1 cut(s)	1442
AgsI	TTSAA	6 cut(s)	151, 729, 773, 887, 1123, 1577
AjiI	CACGTC	1 cut(s)	523
AjnI	CCWGG	2 cut(s)	342, 411
AjuI	GAANNNNNNNTTGG	2 cut(s)	56, 88
AleI	CACNNNNGTG	1 cut(s)	714
AloI	GAACNNNNNNTCC	2 cut(s)	94, 126
AluBI	AGCT	4 cut(s)	818, 1265, 1433, 1573
AluI	AGCT	4 cut(s)	818, 1265, 1433, 1573
Alw26I	GTCTC	6 cut(s)	125, 206, 536, 586, 1040, 1067
AlwI	GGATC	2 cut(s)	101, 1412
Ama87I	CYCGRG	3 cut(s)	53, 939, 1323
AoxI	GGCC	5 cut(s)	375, 409, 1227, 1396, 1491
ApeKI	GCWGC	3 cut(s)	893, 1211, 1265
ApoI	RAATTY	3 cut(s)	400, 541, 804
AsiGI	ACCGGT	1 cut(s)	1442
Asp700I	GAANNNNTTC	2 cut(s)	702, 1118
AspLEI	GCGC	1 cut(s)	1056
AspS9I	GGNCC	3 cut(s)	110, 284, 1480
AsuHPI	GGTGA	4 cut(s)	197, 527, 946, 1234
AsuNHI	GCTAGC	1 cut(s)	1411
AvaI	CYCGRG	3 cut(s)	53, 939, 1323
AvaII	GGWCC	3 cut(s)	110, 284, 1480
BaeI	ACNNNNGTAYC	4 cut(s)	330, 363, 1539, 1572
BarI	GAAGNNNNNNTAC	2 cut(s)	463, 495
BauI	CACGAG	2 cut(s)	837, 978
BbsI	GAAGAC	1 cut(s)	525
BbvI	GCAGC	3 cut(s)	880, 1223, 1252
BccI	CCATC	6 cut(s)	34, 668, 815, 1010, 1223, 1341
BciT130I	CCWGG	2 cut(s)	344, 413
BclI	TGATCA	2 cut(s)	361, 1557
BcoDI	GTCTC	6 cut(s)	125, 206, 536, 586, 1040, 1067
BfaI	CTAG	2 cut(s)	1037, 1412
BfuAI	ACCTGC	1 cut(s)	286
BglII	AGATCT	1 cut(s)	856
BisI	GCNGC	3 cut(s)	894, 1212, 1266
BlsI	GCNGC	3 cut(s)	895, 1213, 1267
Bme1390I	CCNGG	2 cut(s)	344, 413
Bme18I	GGWCC	3 cut(s)	110, 284, 1480
BmeT110I	CYCGRG	3 cut(s)	53, 939, 1323
BmgBI	CACGTC	1 cut(s)	523
BmgT120I	GGNCC	3 cut(s)	110, 284, 1480
BmiI	GGNNCC	1 cut(s)	285
BmrFI	CCNGG	2 cut(s)	344, 413
BmsI	GCATC	3 cut(s)	184, 325, 1015
BmtI	GCTAGC	1 cut(s)	1415
BpiI	GAAGAC	1 cut(s)	525
BplI	GAGNNNNNCTC	6 cut(s)	122, 154, 365, 397, 1064, 1096
BpmI	CTGGAG	1 cut(s)	515
BsaAI	YACGTR	1 cut(s)	1208
BsaBI	GATNNNNATC	1 cut(s)	1562
BsaI	GGTCTC	1 cut(s)	1040
BsaJI	CCNNGG	3 cut(s)	342, 1322, 1483
BsaWI	WCCGGW	2 cut(s)	140, 1442
Bsc4I	CCNNNNNNNGG	4 cut(s)	418, 466, 558, 1489
Bse118I	RCCGGY	1 cut(s)	1442
Bse1I	ACTGG	1 cut(s)	1017
Bse3DI	GCAATG	2 cut(s)	90, 243
Bse8I	GATNNNNATC	1 cut(s)	1562
BseBI	CCWGG	2 cut(s)	344, 413
BseDI	CCNNGG	3 cut(s)	342, 1322, 1483
BseGI	GGATG	4 cut(s)	45, 316, 1021, 1426
BseJI	GATNNNNATC	1 cut(s)	1562
BseLI	CCNNNNNNNGG	4 cut(s)	418, 466, 558, 1489
BseMI	GCAATG	2 cut(s)	90, 243
BseMII	CTCAG	5 cut(s)	212, 495, 599, 767, 1095
BseNI	ACTGG	1 cut(s)	1017
BseXI	GCAGC	3 cut(s)	880, 1223, 1252
BsgI	GTGCAG	3 cut(s)	282, 1062, 1230
Bsh1236I	CGCG	2 cut(s)	688, 1056
Bsh1285I	CGRYCG	1 cut(s)	125
BshFI	GGCC	5 cut(s)	377, 411, 1229, 1398, 1493
BshTI	ACCGGT	1 cut(s)	1442
BsiEI	CGRYCG	1 cut(s)	125
BsiHKCI	CYCGRG	3 cut(s)	53, 939, 1323
BsiSI	CCGG	2 cut(s)	141, 1443
BslI	CCNNNNNNNGG	4 cut(s)	418, 466, 558, 1489
BsmAI	GTCTC	6 cut(s)	125, 206, 536, 586, 1040, 1067
BsmBI	CGTCTC	1 cut(s)	1067
BsmI	GAATGC	1 cut(s)	4
BsnI	GGCC	5 cut(s)	377, 411, 1229, 1398, 1493
Bso31I	GGTCTC	1 cut(s)	1040
BsoBI	CYCGRG	3 cut(s)	53, 939, 1323
Bsp19I	CCATGG	1 cut(s)	1483
BspACI	CCGC	3 cut(s)	1056, 1109, 1202
BspANI	GGCC	5 cut(s)	377, 411, 1229, 1398, 1493
BspCNI	CTCAG	5 cut(s)	211, 496, 598, 766, 1096
BspFNI	CGCG	2 cut(s)	688, 1056
BspLI	GGNNCC	1 cut(s)	285
BspMI	ACCTGC	1 cut(s)	286
BspOI	GCTAGC	1 cut(s)	1415
BspPI	GGATC	2 cut(s)	101, 1412
BspQI	GCTCTTC	2 cut(s)	21, 1255
BspTNI	GGTCTC	1 cut(s)	1040
BsrDI	GCAATG	2 cut(s)	90, 243
BsrFI	RCCGGY	1 cut(s)	1442
BsrI	ACTGG	1 cut(s)	1017
BssAI	RCCGGY	1 cut(s)	1442
BssECI	CCNNGG	3 cut(s)	342, 1322, 1483
BssNAI	GTATAC	1 cut(s)	570
BssSI	CACGAG	2 cut(s)	837, 978
BssT1I	CCWWGG	1 cut(s)	1483
Bst1107I	GTATAC	1 cut(s)	570
Bst2BI	CACGAG	2 cut(s)	837, 978
Bst2UI	CCWGG	2 cut(s)	344, 413
Bst4CI	ACNGT	4 cut(s)	174, 258, 280, 953
Bst6I	CTCTTC	4 cut(s)	21, 236, 333, 1255
BstBAI	YACGTR	1 cut(s)	1208
BstC8I	GCNNGC	2 cut(s)	927, 1413
BstDEI	CTNAG	6 cut(s)	198, 504, 585, 753, 968, 1104
BstDSI	CCRYGG	1 cut(s)	1483
BstENI	CCTNNNNNAGG	1 cut(s)	416
BstF5I	GGATG	4 cut(s)	45, 316, 1021, 1426
BstFNI	CGCG	2 cut(s)	688, 1056
BstHHI	GCGC	1 cut(s)	1056
BstMAI	GTCTC	6 cut(s)	125, 206, 536, 586, 1040, 1067
BstMCI	CGRYCG	1 cut(s)	125
BstMWI	GCNNNNNNNGC	4 cut(s)	64, 1199, 1208, 1370
BstNI	CCWGG	2 cut(s)	344, 413
BstNSI	RCATGY	1 cut(s)	581
BstSCI	CCNGG	2 cut(s)	342, 411
BstUI	CGCG	2 cut(s)	688, 1056
BstV1I	GCAGC	3 cut(s)	880, 1223, 1252
BstV2I	GAAGAC	1 cut(s)	525
BstX2I	RGATCY	2 cut(s)	856, 1417
BstXI	CCANNNNNNTGG	1 cut(s)	1541
BstYI	RGATCY	2 cut(s)	856, 1417
BstZ17I	GTATAC	1 cut(s)	570
BsuRI	GGCC	5 cut(s)	377, 411, 1229, 1398, 1493
BtgI	CCRYGG	1 cut(s)	1483
BtgZI	GCGATG	1 cut(s)	207
BtrI	CACGTC	1 cut(s)	523
BtsCI	GGATG	4 cut(s)	45, 316, 1021, 1426
BtsI	GCAGTG	2 cut(s)	429, 936
BtsIMutI	CAGTG	4 cut(s)	285, 429, 501, 936
BveI	ACCTGC	1 cut(s)	286
Cac8I	GCNNGC	2 cut(s)	927, 1413
CfoI	GCGC	1 cut(s)	1056
Cfr10I	RCCGGY	1 cut(s)	1442
Cfr13I	GGNCC	3 cut(s)	110, 284, 1480
CseI	GACGC	1 cut(s)	677
Csp6I	GTAC	5 cut(s)	246, 1010, 1063, 1205, 1285
CspAI	ACCGGT	1 cut(s)	1442
CspCI	CAANNNNNGTGG	2 cut(s)	78, 113
CviAII	CATG	8 cut(s)	186, 578, 692, 913, 1408, 1484, 1514, 1567
CviQI	GTAC	5 cut(s)	246, 1010, 1063, 1205, 1285
DdeI	CTNAG	6 cut(s)	198, 504, 585, 753, 968, 1104
DraI	TTTAAA	1 cut(s)	789
Eam1104I	CTCTTC	4 cut(s)	21, 236, 333, 1255
EarI	CTCTTC	4 cut(s)	21, 236, 333, 1255
EciI	GGCGGA	1 cut(s)	1124
Eco130I	CCWWGG	1 cut(s)	1483
Eco147I	AGGCCT	2 cut(s)	1229, 1398
Eco31I	GGTCTC	1 cut(s)	1040
Eco47I	GGWCC	3 cut(s)	110, 284, 1480
Eco57I	CTGAAG	4 cut(s)	248, 314, 717, 1005
Eco88I	CYCGRG	3 cut(s)	53, 939, 1323
EcoNI	CCTNNNNNAGG	1 cut(s)	416
EcoRI	GAATTC	1 cut(s)	541
EcoRII	CCWGG	2 cut(s)	342, 411
EcoT14I	CCWWGG	1 cut(s)	1483
EcoT22I	ATGCAT	1 cut(s)	6
ErhI	CCWWGG	1 cut(s)	1483
Esp3I	CGTCTC	1 cut(s)	1067
FaeI	CATG	8 cut(s)	189, 581, 695, 916, 1411, 1487, 1517, 1570
FatI	CATG	8 cut(s)	185, 577, 691, 912, 1407, 1483, 1513, 1566
FbaI	TGATCA	2 cut(s)	361, 1557
FblI	GTMKAC	1 cut(s)	569
Fnu4HI	GCNGC	3 cut(s)	894, 1212, 1266
FokI	GGATG	4 cut(s)	52, 303, 1028, 1413
Fsp4HI	GCNGC	3 cut(s)	894, 1212, 1266
FspBI	CTAG	2 cut(s)	1037, 1412
GlaI	GCGC	1 cut(s)	1055
GluI	GCNGC	3 cut(s)	894, 1212, 1266
GsuI	CTGGAG	1 cut(s)	515
HaeIII	GGCC	5 cut(s)	377, 411, 1229, 1398, 1493
HapII	CCGG	2 cut(s)	141, 1443
HgaI	GACGC	1 cut(s)	677
HhaI	GCGC	1 cut(s)	1056
Hin1II	CATG	8 cut(s)	189, 581, 695, 916, 1411, 1487, 1517, 1570
Hin6I	GCGC	1 cut(s)	1054
HinP1I	GCGC	1 cut(s)	1054
HinfI	GANTC	6 cut(s)	10, 49, 589, 937, 1184, 1457
HpaII	CCGG	2 cut(s)	141, 1443
HphI	GGTGA	4 cut(s)	197, 527, 946, 1234
Hpy166II	GTNNAC	3 cut(s)	570, 901, 949
Hpy188I	TCNGA	4 cut(s)	588, 756, 1105, 1346
Hpy8I	GTNNAC	3 cut(s)	570, 901, 949
HpyAV	CCTTC	6 cut(s)	69, 145, 223, 424, 865, 1219
HpyCH4III	ACNGT	4 cut(s)	174, 258, 280, 953
HpyCH4IV	ACGT	2 cut(s)	522, 1207
HpyF10VI	GCNNNNNNNGC	4 cut(s)	64, 1199, 1208, 1370
HpyF3I	CTNAG	6 cut(s)	198, 504, 585, 753, 968, 1104
HpySE526I	ACGT	2 cut(s)	522, 1207
Hsp92II	CATG	8 cut(s)	189, 581, 695, 916, 1411, 1487, 1517, 1570
HspAI	GCGC	1 cut(s)	1054
Ksp22I	TGATCA	2 cut(s)	361, 1557
LguI	GCTCTTC	2 cut(s)	21, 1255
LmnI	GCTCC	1 cut(s)	1570
Lsp1109I	GCAGC	3 cut(s)	880, 1223, 1252
LweI	GCATC	3 cut(s)	184, 325, 1015
MaeI	CTAG	2 cut(s)	1037, 1412
MaeII	ACGT	2 cut(s)	522, 1207
MaeIII	GTNAC	3 cut(s)	499, 653, 934
MboII	GAAGA	9 cut(s)	38, 253, 320, 347, 439, 530, 710, 1025, 1272
MflI	RGATCY	2 cut(s)	856, 1417
MluCI	AATT	8 cut(s)	400, 441, 541, 768, 804, 1029, 1236, 1539
MluI	ACGCGT	1 cut(s)	686
MlyI	GAGTC	3 cut(s)	4, 931, 1466
MmeI	TCCRAC	2 cut(s)	1069, 1141
Mph1103I	ATGCAT	1 cut(s)	6
MroXI	GAANNNNTTC	2 cut(s)	702, 1118
MseI	TTAA	4 cut(s)	612, 788, 1140, 1467
MslI	CAYNNNNRTG	4 cut(s)	714, 798, 1220, 1518
MspA1I	CMGCKG	1 cut(s)	1433
MspI	CCGG	2 cut(s)	141, 1443
MspR9I	CCNGG	2 cut(s)	344, 413
Mva1269I	GAATGC	1 cut(s)	4
MvaI	CCWGG	2 cut(s)	344, 413
MvnI	CGCG	2 cut(s)	688, 1056
MwoI	GCNNNNNNNGC	4 cut(s)	64, 1199, 1208, 1370
NcoI	CCATGG	1 cut(s)	1483
NheI	GCTAGC	1 cut(s)	1411
NlaIII	CATG	8 cut(s)	189, 581, 695, 916, 1411, 1487, 1517, 1570
NlaIV	GGNNCC	1 cut(s)	285
NmuCI	GTSAC	2 cut(s)	499, 934
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	1 cut(s)	581
OliI	CACNNNNGTG	1 cut(s)	714
PaeR7I	CTCGAG	1 cut(s)	53
PceI	AGGCCT	2 cut(s)	1229, 1398
PciSI	GCTCTTC	2 cut(s)	21, 1255
PcsI	WCGNNNNNNNCGW	1 cut(s)	712
PctI	GAATGC	1 cut(s)	4
PdmI	GAANNNNTTC	2 cut(s)	702, 1118
PfeI	GAWTC	3 cut(s)	49, 589, 1184
PinAI	ACCGGT	1 cut(s)	1442
PkrI	GCNGC	3 cut(s)	895, 1213, 1267
Ple19I	CGATCG	1 cut(s)	125
PleI	GAGTC	3 cut(s)	4, 931, 1465
PpsI	GAGTC	3 cut(s)	4, 931, 1465
Ppu21I	YACGTR	1 cut(s)	1208
Psp6I	CCWGG	2 cut(s)	342, 411
PspGI	CCWGG	2 cut(s)	342, 411
PspN4I	GGNNCC	1 cut(s)	285
PspPI	GGNCC	3 cut(s)	110, 284, 1480
PsuI	RGATCY	2 cut(s)	856, 1417
PvuI	CGATCG	1 cut(s)	125
PvuII	CAGCTG	1 cut(s)	1433
RsaI	GTAC	5 cut(s)	247, 1011, 1064, 1206, 1286
RsaNI	GTAC	5 cut(s)	246, 1010, 1063, 1205, 1285
RseI	CAYNNNNRTG	4 cut(s)	714, 798, 1220, 1518
SapI	GCTCTTC	2 cut(s)	21, 1255
SaqAI	TTAA	4 cut(s)	612, 788, 1140, 1467
SatI	GCNGC	3 cut(s)	894, 1212, 1266
Sau96I	GGNCC	3 cut(s)	110, 284, 1480
SchI	GAGTC	3 cut(s)	4, 931, 1466
ScrFI	CCNGG	2 cut(s)	344, 413
SfaNI	GCATC	3 cut(s)	184, 325, 1015
Sfr274I	CTCGAG	1 cut(s)	53
SinI	GGWCC	3 cut(s)	110, 284, 1480
SlaI	CTCGAG	1 cut(s)	53
SmiMI	CAYNNNNRTG	4 cut(s)	714, 798, 1220, 1518
SmlI	CTYRAG	1 cut(s)	53
SmoI	CTYRAG	1 cut(s)	53
Sse9I	AATT	8 cut(s)	400, 441, 541, 768, 804, 1029, 1236, 1539
SseBI	AGGCCT	2 cut(s)	1229, 1398
SsiI	CCGC	3 cut(s)	1056, 1109, 1202
SspMI	CTAG	2 cut(s)	1037, 1412
StuI	AGGCCT	2 cut(s)	1229, 1398
StyD4I	CCNGG	2 cut(s)	342, 411
StyI	CCWWGG	1 cut(s)	1483
TaaI	ACNGT	4 cut(s)	174, 258, 280, 953
TaiI	ACGT	2 cut(s)	525, 1210
TaqI	TCGA	5 cut(s)	8, 47, 54, 663, 1423
TaqII	GACCGA	1 cut(s)	974
TasI	AATT	8 cut(s)	400, 441, 541, 768, 804, 1029, 1236, 1539
TatI	WGTACW	2 cut(s)	1009, 1284
TfiI	GAWTC	3 cut(s)	49, 589, 1184
Tru1I	TTAA	4 cut(s)	612, 788, 1140, 1467
Tru9I	TTAA	4 cut(s)	612, 788, 1140, 1467
TscAI	CASTG	4 cut(s)	285, 429, 508, 936
TseFI	GTSAC	2 cut(s)	499, 934
TseI	GCWGC	3 cut(s)	893, 1211, 1265
Tsp45I	GTSAC	2 cut(s)	499, 934
TspDTI	ATGAA	3 cut(s)	174, 254, 816
TspRI	CASTG	4 cut(s)	285, 429, 508, 936
VpaK11BI	GGWCC	3 cut(s)	110, 284, 1480
XagI	CCTNNNNNAGG	1 cut(s)	416
XapI	RAATTY	3 cut(s)	400, 541, 804
XceI	RCATGY	1 cut(s)	581
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1009
XhoI	CTCGAG	1 cut(s)	53
XmiI	GTMKAC	1 cut(s)	569
XmnI	GAANNNNTTC	2 cut(s)	702, 1118
XspI	CTAG	2 cut(s)	1037, 1412
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.