MD07G1072900.v1.1

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001999
Physical Location & Seq
Forward (+)
6900660 .. 6910159
9500 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1072900.v1.1.491

Sequence Viewer

Length: 1584 bp
ATGCATTCGAGTCACTTGCTTCTGGAAGAGCCGATCAGGATGGCTTCGATCCTCGAGCCTTCCAAGCCTACATTCTTTCCGGCAATGACAAGGATTGTGGGAACGCTTGGTCCAAAATCACAATCAGTCGAGACAATCTCCGGTTGCTTGAAGGCAGGAATGTCTGTGGCACGGTTTGATTTTTCATGGGGCGATGCTCAGTTTCACCAAGAGACATTGGGAAACCTGAAGGTTGCAATGAAGAGTACCAATAAACTGTGTGCAGTAATGCTAGACACAGTGGGTCCAGAGTTGCAGGTAGTAAATAAAAGTGAGCATCCCATATCTCTTCAGGAAGATACCCTGGTTGTCCTAACACCTGATCAAGACCAAGAGGCCACCTCAAATCTACTACCCGTAAATTTTAGTGGCCTGGCAAGGGCAGTGAAGAAGGGAGATACAATTTTTATTGGTCAATACCTCTTTACGGGGAGTGAAACTACTTCTGTGTGGCTGGAGGTCACTGAGGTGAATGGTGAAGATGTGGTTTGTCTCATAAAGAATTCTGCTACCTTGGCAGGGTCATTGTATACTTTGAATGTCTCTCAGATTCGTATTGATCTGCCTACACTTACCGATAAAGATAAGGAGATCATAAGCACTTGGGGTGTTCGTAACACCATCGACTTCCTCTCATTATCATATACGCGTCATGCTGAAGATGTTCGCCATGCCCGTGAGTTTCTTTCAAAGTTAGGCGACCTCAACCAAACTCAGATTTTTGCAAAAATTGAAAATGTAGAGGGGTTAAATCATTTTGATGAAATTTTGCAAGAAGCTGATGGTATTATCGTCTCTCGTGGAATTCTGGGGATAGATCTCCCACCAGAGAAGGTATTTTTATTTCAAAAAGCTGCTGTTTACAAGTGCAACATGGCTGGAAAGCCAGCAGTGGTGACTCGGGTTGTGGACAGTATGACCGACAACTTAAGACCTACTCGTGCTGAAGCAACTGATGTTGCCAACGCTGTACTGGATGGAAGTGATGCAATTCTTCTAGGTGCAGAGACCCTGCGCGGTTTGTACCCTGTTGAGACGATCTCCATTGTTGGAAAGATTTGCTCTGAGGCGGAGAAAGTTTTCAATCAAGATTTGTATTTTAAGAGGGCTGTCAAATATGTTGGAGAACCAATGACCCACTTGGAATCTATTGCTTCCTCTGCGGTACGTGCAGCCATCAAGGTGAAGGCCTCTGTAATTATTTGCTTCACTACATCTGGAAGAGCTGCAAGGTTGATTGCAAAGTACAGACCAACAATGCCTGTGCTTTCTGTTGTCATTCCTCGGGTCAAGACAAATCAACTCCGATGGACATTTTCTGGTGCTTTTGAGGCAAGGCAATCGCTCATTGTTAGAGGCATTTTTCCCATGCTAGCGGATCCTCGACATCCAGCTGAAATAACCGTTGCAACAAATGAGTCCATTCTTAAAATCGCTTTGGACCATGGCAAGGCCACTGGTGTCGTAAAATCACATGACCGTGTGGTCATTTGCCAAAAAATTGGCGATACTTCTGTGATCAAGATCATGGAGCTTGAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

528

Amino Acids

57.75

Weight (kDa)

6.17

Isoelectric Point (pI)

27.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 30 - 360 1.4e-88 Pyruvate kinase, barrel domain
PK_C PF02887 395 - 522 2.7e-22 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1523
Acc36I ACCTGC 1 cut(s) 286
AccI GTMKAC 1 cut(s) 569
AccII CGCG 2 cut(s) 688, 1056
AciI CCGC 4 cut(s) 1056, 1109, 1202, 1415
AclWI GGATC 3 cut(s) 43, 1412, 1425
AcsI RAATTY 4 cut(s) 400, 541, 804, 843
AcuI CTGAAG 4 cut(s) 248, 314, 717, 1005
AfaI GTAC 5 cut(s) 247, 1011, 1064, 1206, 1286
AfiI CCNNNNNNNGG 4 cut(s) 418, 466, 558, 1489
AflII CTTAAG 1 cut(s) 967
AflIII ACRYGT 1 cut(s) 686
AgsI TTSAA 7 cut(s) 151, 577, 729, 773, 887, 1123, 1577
AjnI CCWGG 2 cut(s) 342, 411
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AleI CACNNNNGTG 1 cut(s) 506
AloI GAACNNNNNNTCC 2 cut(s) 94, 126
AluBI AGCT 5 cut(s) 818, 893, 1265, 1433, 1573
AluI AGCT 5 cut(s) 818, 893, 1265, 1433, 1573
Alw26I GTCTC 7 cut(s) 125, 206, 536, 586, 838, 1040, 1067
AlwI GGATC 3 cut(s) 43, 1412, 1425
Ama87I CYCGRG 3 cut(s) 53, 939, 1323
AoxI GGCC 4 cut(s) 375, 409, 1227, 1491
ApeKI GCWGC 3 cut(s) 893, 1211, 1265
ApoI RAATTY 4 cut(s) 400, 541, 804, 843
Asp700I GAANNNNTTC 2 cut(s) 702, 1118
AspLEI GCGC 1 cut(s) 1056
AspS9I GGNCC 3 cut(s) 110, 284, 1480
AsuHPI GGTGA 5 cut(s) 197, 520, 527, 946, 1234
AsuNHI GCTAGC 1 cut(s) 1411
AvaI CYCGRG 3 cut(s) 53, 939, 1323
AvaII GGWCC 3 cut(s) 110, 284, 1480
BaeI ACNNNNGTAYC 4 cut(s) 330, 363, 1539, 1572
BamHI GGATCC 1 cut(s) 1417
BauI CACGAG 2 cut(s) 837, 978
BbvI GCAGC 3 cut(s) 880, 1223, 1252
BccI CCATC 6 cut(s) 34, 668, 815, 1010, 1223, 1341
BcgI CGANNNNNNTGC 3 cut(s) 32, 1362, 1396
BciT130I CCWGG 2 cut(s) 344, 413
BclI TGATCA 2 cut(s) 361, 1557
BcoDI GTCTC 7 cut(s) 125, 206, 536, 586, 838, 1040, 1067
BfaI CTAG 3 cut(s) 272, 1037, 1412
BfrI CTTAAG 1 cut(s) 967
BfuAI ACCTGC 1 cut(s) 286
BglII AGATCT 1 cut(s) 856
BisI GCNGC 3 cut(s) 894, 1212, 1266
BlsI GCNGC 3 cut(s) 895, 1213, 1267
Bme1390I CCNGG 2 cut(s) 344, 413
Bme18I GGWCC 3 cut(s) 110, 284, 1480
BmeT110I CYCGRG 3 cut(s) 53, 939, 1323
BmgT120I GGNCC 3 cut(s) 110, 284, 1480
BmiI GGNNCC 2 cut(s) 285, 1419
BmrFI CCNGG 2 cut(s) 344, 413
BmsI GCATC 3 cut(s) 184, 325, 1015
BmtI GCTAGC 1 cut(s) 1415
BplI GAGNNNNNCTC 6 cut(s) 122, 154, 365, 397, 1064, 1096
BpmI CTGGAG 1 cut(s) 515
BsaAI YACGTR 1 cut(s) 1208
BsaBI GATNNNNATC 1 cut(s) 1562
BsaI GGTCTC 1 cut(s) 1040
BsaJI CCNNGG 4 cut(s) 342, 552, 1322, 1483
BsaWI WCCGGW 1 cut(s) 140
Bsc4I CCNNNNNNNGG 4 cut(s) 418, 466, 558, 1489
Bse1I ACTGG 2 cut(s) 1017, 1501
Bse3DI GCAATG 2 cut(s) 90, 243
Bse8I GATNNNNATC 1 cut(s) 1562
BseBI CCWGG 2 cut(s) 344, 413
BseDI CCNNGG 4 cut(s) 342, 552, 1322, 1483
BseGI GGATG 4 cut(s) 45, 316, 1021, 1426
BseJI GATNNNNATC 1 cut(s) 1562
BseLI CCNNNNNNNGG 4 cut(s) 418, 466, 558, 1489
BseMI GCAATG 2 cut(s) 90, 243
BseMII CTCAG 5 cut(s) 212, 495, 599, 767, 1095
BseNI ACTGG 2 cut(s) 1017, 1501
BseXI GCAGC 3 cut(s) 880, 1223, 1252
BsgI GTGCAG 3 cut(s) 282, 1062, 1230
Bsh1236I CGCG 2 cut(s) 688, 1056
BshFI GGCC 4 cut(s) 377, 411, 1229, 1493
BsiHKCI CYCGRG 3 cut(s) 53, 939, 1323
BsiSI CCGG 2 cut(s) 80, 141
BslI CCNNNNNNNGG 4 cut(s) 418, 466, 558, 1489
BsmAI GTCTC 7 cut(s) 125, 206, 536, 586, 838, 1040, 1067
BsmBI CGTCTC 2 cut(s) 838, 1067
BsmI GAATGC 1 cut(s) 4
BsnI GGCC 4 cut(s) 377, 411, 1229, 1493
Bso31I GGTCTC 1 cut(s) 1040
BsoBI CYCGRG 3 cut(s) 53, 939, 1323
Bsp19I CCATGG 1 cut(s) 1483
BspACI CCGC 4 cut(s) 1056, 1109, 1202, 1415
BspANI GGCC 4 cut(s) 377, 411, 1229, 1493
BspCNI CTCAG 5 cut(s) 211, 496, 598, 766, 1096
BspFNI CGCG 2 cut(s) 688, 1056
BspLI GGNNCC 2 cut(s) 285, 1419
BspMI ACCTGC 1 cut(s) 286
BspOI GCTAGC 1 cut(s) 1415
BspPI GGATC 3 cut(s) 43, 1412, 1425
BspQI GCTCTTC 2 cut(s) 21, 1255
BspTI CTTAAG 1 cut(s) 967
BspTNI GGTCTC 1 cut(s) 1040
BsrDI GCAATG 2 cut(s) 90, 243
BsrI ACTGG 2 cut(s) 1017, 1501
BssECI CCNNGG 4 cut(s) 342, 552, 1322, 1483
BssNAI GTATAC 1 cut(s) 570
BssSI CACGAG 2 cut(s) 837, 978
BssT1I CCWWGG 2 cut(s) 552, 1483
Bst1107I GTATAC 1 cut(s) 570
Bst2BI CACGAG 2 cut(s) 837, 978
Bst2UI CCWGG 2 cut(s) 344, 413
Bst4CI ACNGT 6 cut(s) 174, 258, 280, 953, 1444, 1520
Bst6I CTCTTC 4 cut(s) 21, 236, 333, 1255
BstAFI CTTAAG 1 cut(s) 967
BstBAI YACGTR 1 cut(s) 1208
BstC8I GCNNGC 2 cut(s) 927, 1413
BstDEI CTNAG 5 cut(s) 198, 504, 585, 753, 1104
BstDSI CCRYGG 1 cut(s) 1483
BstF5I GGATG 4 cut(s) 45, 316, 1021, 1426
BstFNI CGCG 2 cut(s) 688, 1056
BstHHI GCGC 1 cut(s) 1056
BstMAI GTCTC 7 cut(s) 125, 206, 536, 586, 838, 1040, 1067
BstMWI GCNNNNNNNGC 5 cut(s) 64, 554, 1199, 1208, 1370
BstNI CCWGG 2 cut(s) 344, 413
BstSCI CCNGG 2 cut(s) 342, 411
BstUI CGCG 2 cut(s) 688, 1056
BstV1I GCAGC 3 cut(s) 880, 1223, 1252
BstX2I RGATCY 2 cut(s) 856, 1417
BstXI CCANNNNNNTGG 1 cut(s) 1541
BstYI RGATCY 2 cut(s) 856, 1417
BstZ17I GTATAC 1 cut(s) 570
BsuRI GGCC 4 cut(s) 377, 411, 1229, 1493
BtgI CCRYGG 1 cut(s) 1483
BtgZI GCGATG 1 cut(s) 207
BtsCI GGATG 4 cut(s) 45, 316, 1021, 1426
BtsI GCAGTG 2 cut(s) 429, 936
BtsIMutI CAGTG 5 cut(s) 285, 429, 501, 936, 1494
BveI ACCTGC 1 cut(s) 286
Cac8I GCNNGC 2 cut(s) 927, 1413
CfoI GCGC 1 cut(s) 1056
Cfr13I GGNCC 3 cut(s) 110, 284, 1480
CseI GACGC 1 cut(s) 677
Csp6I GTAC 5 cut(s) 246, 1010, 1063, 1205, 1285
CspCI CAANNNNNGTGG 2 cut(s) 78, 113
CviAII CATG 8 cut(s) 186, 692, 710, 913, 1408, 1484, 1514, 1567
CviQI GTAC 5 cut(s) 246, 1010, 1063, 1205, 1285
DdeI CTNAG 5 cut(s) 198, 504, 585, 753, 1104
DrdI GACNNNNNNGTC 1 cut(s) 1523
DseDI GACNNNNNNGTC 1 cut(s) 1523
Eam1104I CTCTTC 4 cut(s) 21, 236, 333, 1255
EarI CTCTTC 4 cut(s) 21, 236, 333, 1255
EciI GGCGGA 1 cut(s) 1124
Eco130I CCWWGG 2 cut(s) 552, 1483
Eco147I AGGCCT 1 cut(s) 1229
Eco31I GGTCTC 1 cut(s) 1040
Eco47I GGWCC 3 cut(s) 110, 284, 1480
Eco57I CTGAAG 4 cut(s) 248, 314, 717, 1005
Eco88I CYCGRG 3 cut(s) 53, 939, 1323
EcoRI GAATTC 2 cut(s) 541, 843
EcoRII CCWGG 2 cut(s) 342, 411
EcoT14I CCWWGG 2 cut(s) 552, 1483
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 552, 1483
Esp3I CGTCTC 2 cut(s) 838, 1067
FaeI CATG 8 cut(s) 189, 695, 713, 916, 1411, 1487, 1517, 1570
FatI CATG 8 cut(s) 185, 691, 709, 912, 1407, 1483, 1513, 1566
FbaI TGATCA 2 cut(s) 361, 1557
FblI GTMKAC 1 cut(s) 569
Fnu4HI GCNGC 3 cut(s) 894, 1212, 1266
FokI GGATG 4 cut(s) 52, 303, 1028, 1413
Fsp4HI GCNGC 3 cut(s) 894, 1212, 1266
FspBI CTAG 3 cut(s) 272, 1037, 1412
GlaI GCGC 1 cut(s) 1055
GluI GCNGC 3 cut(s) 894, 1212, 1266
GsuI CTGGAG 1 cut(s) 515
HaeIII GGCC 4 cut(s) 377, 411, 1229, 1493
HapII CCGG 2 cut(s) 80, 141
HgaI GACGC 1 cut(s) 677
HhaI GCGC 1 cut(s) 1056
Hin1II CATG 8 cut(s) 189, 695, 713, 916, 1411, 1487, 1517, 1570
Hin6I GCGC 1 cut(s) 1054
HinP1I GCGC 1 cut(s) 1054
HinfI GANTC 5 cut(s) 10, 589, 937, 1184, 1457
HpaII CCGG 2 cut(s) 80, 141
HphI GGTGA 5 cut(s) 197, 520, 527, 946, 1234
Hpy166II GTNNAC 3 cut(s) 570, 901, 949
Hpy188I TCNGA 4 cut(s) 588, 756, 1105, 1346
Hpy8I GTNNAC 3 cut(s) 570, 901, 949
HpyAV CCTTC 6 cut(s) 69, 145, 223, 424, 865, 1219
HpyCH4III ACNGT 6 cut(s) 174, 258, 280, 953, 1444, 1520
HpyCH4IV ACGT 1 cut(s) 1207
HpyF10VI GCNNNNNNNGC 5 cut(s) 64, 554, 1199, 1208, 1370
HpyF3I CTNAG 5 cut(s) 198, 504, 585, 753, 1104
HpySE526I ACGT 1 cut(s) 1207
Hsp92II CATG 8 cut(s) 189, 695, 713, 916, 1411, 1487, 1517, 1570
HspAI GCGC 1 cut(s) 1054
Ksp22I TGATCA 2 cut(s) 361, 1557
LguI GCTCTTC 2 cut(s) 21, 1255
LmnI GCTCC 1 cut(s) 1570
Lsp1109I GCAGC 3 cut(s) 880, 1223, 1252
LweI GCATC 3 cut(s) 184, 325, 1015
MaeI CTAG 3 cut(s) 272, 1037, 1412
MaeII ACGT 1 cut(s) 1207
MaeIII GTNAC 4 cut(s) 11, 499, 653, 934
MboII GAAGA 9 cut(s) 38, 253, 320, 347, 439, 530, 710, 1025, 1272
MflI RGATCY 2 cut(s) 856, 1417
MluCI AATT 9 cut(s) 400, 441, 541, 768, 804, 843, 1029, 1236, 1539
MluI ACGCGT 1 cut(s) 686
MlyI GAGTC 3 cut(s) 19, 931, 1466
MmeI TCCRAC 2 cut(s) 1069, 1141
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 702, 1118
MseI TTAA 4 cut(s) 788, 968, 1140, 1467
MslI CAYNNNNRTG 5 cut(s) 506, 714, 798, 1220, 1518
MspA1I CMGCKG 1 cut(s) 1433
MspCI CTTAAG 1 cut(s) 967
MspI CCGG 2 cut(s) 80, 141
MspR9I CCNGG 2 cut(s) 344, 413
Mva1269I GAATGC 1 cut(s) 4
MvaI CCWGG 2 cut(s) 344, 413
MvnI CGCG 2 cut(s) 688, 1056
MwoI GCNNNNNNNGC 5 cut(s) 64, 554, 1199, 1208, 1370
NcoI CCATGG 1 cut(s) 1483
NheI GCTAGC 1 cut(s) 1411
NlaIII CATG 8 cut(s) 189, 695, 713, 916, 1411, 1487, 1517, 1570
NlaIV GGNNCC 2 cut(s) 285, 1419
NmuCI GTSAC 3 cut(s) 11, 499, 934
NsiI ATGCAT 1 cut(s) 6
OliI CACNNNNGTG 1 cut(s) 506
PaeR7I CTCGAG 1 cut(s) 53
PceI AGGCCT 1 cut(s) 1229
PciSI GCTCTTC 2 cut(s) 21, 1255
PcsI WCGNNNNNNNCGW 1 cut(s) 712
PctI GAATGC 1 cut(s) 4
PdmI GAANNNNTTC 2 cut(s) 702, 1118
PfeI GAWTC 2 cut(s) 589, 1184
PkrI GCNGC 3 cut(s) 895, 1213, 1267
PleI GAGTC 3 cut(s) 18, 931, 1465
PpsI GAGTC 3 cut(s) 18, 931, 1465
Ppu21I YACGTR 1 cut(s) 1208
Psp6I CCWGG 2 cut(s) 342, 411
PspGI CCWGG 2 cut(s) 342, 411
PspN4I GGNNCC 2 cut(s) 285, 1419
PspPI GGNCC 3 cut(s) 110, 284, 1480
PspXI VCTCGAGB 1 cut(s) 53
PsuI RGATCY 2 cut(s) 856, 1417
PvuII CAGCTG 1 cut(s) 1433
RsaI GTAC 5 cut(s) 247, 1011, 1064, 1206, 1286
RsaNI GTAC 5 cut(s) 246, 1010, 1063, 1205, 1285
RseI CAYNNNNRTG 5 cut(s) 506, 714, 798, 1220, 1518
SapI GCTCTTC 2 cut(s) 21, 1255
SaqAI TTAA 4 cut(s) 788, 968, 1140, 1467
SatI GCNGC 3 cut(s) 894, 1212, 1266
Sau96I GGNCC 3 cut(s) 110, 284, 1480
SchI GAGTC 3 cut(s) 19, 931, 1466
ScrFI CCNGG 2 cut(s) 344, 413
SfaNI GCATC 3 cut(s) 184, 325, 1015
Sfr274I CTCGAG 1 cut(s) 53
SinI GGWCC 3 cut(s) 110, 284, 1480
SlaI CTCGAG 1 cut(s) 53
SmiMI CAYNNNNRTG 5 cut(s) 506, 714, 798, 1220, 1518
SmlI CTYRAG 2 cut(s) 53, 967
SmoI CTYRAG 2 cut(s) 53, 967
Sse9I AATT 9 cut(s) 400, 441, 541, 768, 804, 843, 1029, 1236, 1539
SseBI AGGCCT 1 cut(s) 1229
SsiI CCGC 4 cut(s) 1056, 1109, 1202, 1415
SspMI CTAG 3 cut(s) 272, 1037, 1412
StuI AGGCCT 1 cut(s) 1229
StyD4I CCNGG 2 cut(s) 342, 411
StyI CCWWGG 2 cut(s) 552, 1483
TaaI ACNGT 6 cut(s) 174, 258, 280, 953, 1444, 1520
TaiI ACGT 1 cut(s) 1210
TaqI TCGA 6 cut(s) 8, 47, 54, 129, 663, 1423
TaqII GACCGA 1 cut(s) 974
TasI AATT 9 cut(s) 400, 441, 541, 768, 804, 843, 1029, 1236, 1539
TatI WGTACW 2 cut(s) 1009, 1284
TfiI GAWTC 2 cut(s) 589, 1184
Tru1I TTAA 4 cut(s) 788, 968, 1140, 1467
Tru9I TTAA 4 cut(s) 788, 968, 1140, 1467
TscAI CASTG 5 cut(s) 285, 429, 508, 936, 1501
TseFI GTSAC 3 cut(s) 11, 499, 934
TseI GCWGC 3 cut(s) 893, 1211, 1265
Tsp45I GTSAC 3 cut(s) 11, 499, 934
TspDTI ATGAA 3 cut(s) 174, 254, 816
TspRI CASTG 5 cut(s) 285, 429, 508, 936, 1501
Vha464I CTTAAG 1 cut(s) 967
VpaK11BI GGWCC 3 cut(s) 110, 284, 1480
XapI RAATTY 4 cut(s) 400, 541, 804, 843
XcmI CCANNNNNNNNNTGG 1 cut(s) 1009
XhoI CTCGAG 1 cut(s) 53
XmiI GTMKAC 1 cut(s) 569
XmnI GAANNNNTTC 2 cut(s) 702, 1118
XspI CTAG 3 cut(s) 272, 1037, 1412
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.