pycom07g05630

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001999
Physical Location & Seq
Forward (+)
4900281 .. 4909786
9506 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g05630.10

Sequence Viewer

Length: 1734 bp
ATGCAAATGGACTTAACCGGTAATTTCCGCAAGCGTACAAAAGACGCTAATCAACATTTCTCGCACTTGCTCAACAGAACATTTTCCACAAGCAGTTGCAGTTGCAGTTGCTGTTGCTCGTCTTCTTCTTCTTCTTCGTCGGCGGACAAAATGCATTCGACTCACTTGTTTCTGGAAGAGCCGATCAGGATGGCTTCGATTCTCGAGCCTTCCAAGCCTACATTCTTCCCGGCAATGACAAGGATCGTGGGAACGCTTGGTCCAAAATCACGATCGGTTGAGACAATCTCTGGTTGCTTGAAGGCAGGAATGTCTGTGGCACGGTTTGATTTTTCATGGGGCGATGCTCAGTTTCACCAAGAGACATTGGGAAACCTGAAGGTTGCAATGAAGAGTACCAATAAACTGTGTGCAGTAATGCTAGACACGGTGGGTCCAGAGTTGCAGGTAGTGAATAAAAGTGAGCATCCCATATCTCTTCAGGAAGATACCCTGGTTGTCCTAACACCCGATCAAGACCAAGAGGCCACCTCAAATCAACTACCCGTCAATTTTAGTGGCCTGGCAAAGGCAGTGAAGAAGGGAGATACAATTTTTATTGGTCAATACCTCTTTACGGGAAGTGAAACTACTTCTGTGTGGCTGGAGGTCACTGAGGTGAATGGTGAAGATGTGGTTTGTCTCATAAAGAATTCTGCTACCTTGGCAGGGTCATTGTATACTTTGCATGTCTCTCAGATTCGTATTGATCTGCCTACACTTAACGATAAAGATAAGGAGGTCATAAGCACTTGGGGTGTTCGTAACACCATCGACTTCCTCTCATTATCATATACGCGTCATGCTGAAGATGTTCGCCACGCCCGTGAGTTTCTTTCAAAGTTAGGCAACCTCAACCAAACTCAGATTTTTGCAAAAATTGAAAATGTAGAGGGGTTAATTCATTTTGATGAAATTTTGCAAGAAGCTGATGGTATTATCCTCTCTCGTGGAAATCTGGGGATAGATCTCCCACCAGAGAAGCTATTTTTATTTCAAAAGGCTGCTGTTTACAAGTGCAACATGGCTGGAAAGCCAGCAGTGGTGACTCGGGTTGTGGACAGTATGACCGACAGCCTAAGACCTACTCGTGCTGAAGCAACTGATGTTGCCAATGCTGTACTGGATGGAAGTGATGCAATTCTTCTAGGTGCAGAGACCCTGCGCGGTTTGTACCCTGTTGAGACGATCTCCACTGTTGGAAAGATTTGCTCTGAGGCGGAGAAAGTTTTTAATCAAGATTTGTATTTTAAGAGGGCTGTCAAATATGTTGGAGAACCAATGACCCACTTGGAATCTATTGCTTCCTCTGCGGTACGTGCAGCCATCAAGGTGAAGGCCTCTGTAATTATTTGCTTCACTACATCTGGAAGAGCTGCAAGGTTGATTGCAAAGTACAGACCAACAATGCCTGTGCTTTCTGTTGTCATACCTCGGGTCAAGACAAATCAACTCCGATGGACATTTTCTGGTGCTTTTGAGGCAAGGCAATCACTCATTGTTAGAGGCCTTTTTCCCATGCTAGCAGATCCTCGACATCCAGCTGAAACTACCGGTGCAACAAATGAGTCAATTCTTAAAATCGCTTTGGACCATGGCAAGGCCATTGGTGTCGTAAAATCACATGATCGTGTGGTTATTTGCCAAAAAATTGGCGACACTTCTGTGATCAAGATCATGGAGCTTGAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

578

Amino Acids

63.24

Weight (kDa)

7.2

Isoelectric Point (pI)

29.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 436
AccI GTMKAC 1 cut(s) 719
AccII CGCG 2 cut(s) 838, 1206
AciI CCGC 5 cut(s) 28, 143, 1206, 1259, 1352
AclWI GGATC 2 cut(s) 251, 1562
AcsI RAATTY 2 cut(s) 691, 954
AcuI CTGAAG 4 cut(s) 398, 464, 867, 1155
AfaI GTAC 6 cut(s) 37, 397, 1161, 1214, 1356, 1436
AfiI CCNNNNNNNGG 4 cut(s) 568, 616, 708, 1639
AflIII ACRYGT 1 cut(s) 836
AgeI ACCGGT 2 cut(s) 17, 1592
AgsI TTSAA 5 cut(s) 301, 879, 923, 1037, 1727
AjnI CCWGG 2 cut(s) 492, 561
AjuI GAANNNNNNNTTGG 2 cut(s) 206, 238
AleI CACNNNNGTG 3 cut(s) 656, 864, 1703
AloI GAACNNNNNNTCC 2 cut(s) 244, 276
AluBI AGCT 5 cut(s) 968, 1024, 1415, 1583, 1723
AluI AGCT 5 cut(s) 968, 1024, 1415, 1583, 1723
Alw26I GTCTC 6 cut(s) 275, 356, 686, 736, 1190, 1217
AlwI GGATC 2 cut(s) 251, 1562
AlwNI CAGNNNCTG 1 cut(s) 111
Ama87I CYCGRG 3 cut(s) 203, 1089, 1473
AoxI GGCC 5 cut(s) 525, 559, 1377, 1546, 1641
ApeKI GCWGC 3 cut(s) 1043, 1361, 1415
ApoI RAATTY 2 cut(s) 691, 954
AsiGI ACCGGT 2 cut(s) 17, 1592
Asp700I GAANNNNTTC 2 cut(s) 82, 852
AspLEI GCGC 1 cut(s) 1206
AspS9I GGNCC 3 cut(s) 260, 434, 1630
AsuC2I CCSGG 1 cut(s) 230
AsuHPI GGTGA 5 cut(s) 347, 670, 677, 1096, 1384
AsuNHI GCTAGC 1 cut(s) 1561
AvaI CYCGRG 3 cut(s) 203, 1089, 1473
AvaII GGWCC 3 cut(s) 260, 434, 1630
BaeI ACNNNNGTAYC 2 cut(s) 480, 513
BarI GAAGNNNNNNTAC 2 cut(s) 613, 645
BauI CACGAG 2 cut(s) 987, 1128
BbsI GAAGAC 1 cut(s) 114
BbvI GCAGC 3 cut(s) 1030, 1373, 1402
BccI CCATC 6 cut(s) 184, 818, 965, 1160, 1373, 1491
BciT130I CCWGG 2 cut(s) 494, 563
BclI TGATCA 1 cut(s) 1707
BcnI CCSGG 1 cut(s) 230
BcoDI GTCTC 6 cut(s) 275, 356, 686, 736, 1190, 1217
BfaI CTAG 3 cut(s) 422, 1187, 1562
BfuAI ACCTGC 1 cut(s) 436
BglII AGATCT 1 cut(s) 1006
BisI GCNGC 3 cut(s) 1044, 1362, 1416
BlsI GCNGC 3 cut(s) 1045, 1363, 1417
Bme1390I CCNGG 3 cut(s) 230, 494, 563
Bme18I GGWCC 3 cut(s) 260, 434, 1630
BmeT110I CYCGRG 3 cut(s) 203, 1089, 1473
BmgT120I GGNCC 3 cut(s) 260, 434, 1630
BmiI GGNNCC 1 cut(s) 435
BmrFI CCNGG 3 cut(s) 230, 494, 563
BmsI GCATC 3 cut(s) 334, 475, 1165
BmtI GCTAGC 1 cut(s) 1565
BpiI GAAGAC 1 cut(s) 114
BplI GAGNNNNNCTC 6 cut(s) 272, 304, 515, 547, 1214, 1246
BpmI CTGGAG 1 cut(s) 665
BpuMI CCSGG 1 cut(s) 230
BsaAI YACGTR 1 cut(s) 1358
BsaBI GATNNNNATC 1 cut(s) 1712
BsaI GGTCTC 1 cut(s) 1190
BsaJI CCNNGG 4 cut(s) 492, 702, 1472, 1633
BsaWI WCCGGW 2 cut(s) 17, 1592
Bsc4I CCNNNNNNNGG 4 cut(s) 568, 616, 708, 1639
Bse118I RCCGGY 2 cut(s) 17, 1592
Bse1I ACTGG 1 cut(s) 1167
Bse3DI GCAATG 2 cut(s) 240, 393
Bse8I GATNNNNATC 1 cut(s) 1712
BseBI CCWGG 2 cut(s) 494, 563
BseDI CCNNGG 4 cut(s) 492, 702, 1472, 1633
BseGI GGATG 4 cut(s) 195, 466, 1171, 1576
BseJI GATNNNNATC 1 cut(s) 1712
BseLI CCNNNNNNNGG 4 cut(s) 568, 616, 708, 1639
BseMI GCAATG 2 cut(s) 240, 393
BseMII CTCAG 5 cut(s) 362, 645, 749, 917, 1245
BseNI ACTGG 1 cut(s) 1167
BseXI GCAGC 3 cut(s) 1030, 1373, 1402
BsgI GTGCAG 3 cut(s) 432, 1212, 1380
Bsh1236I CGCG 2 cut(s) 838, 1206
Bsh1285I CGRYCG 1 cut(s) 275
BshFI GGCC 5 cut(s) 527, 561, 1379, 1548, 1643
BshTI ACCGGT 2 cut(s) 17, 1592
BsiEI CGRYCG 1 cut(s) 275
BsiHKCI CYCGRG 3 cut(s) 203, 1089, 1473
BsiSI CCGG 3 cut(s) 18, 230, 1593
BslI CCNNNNNNNGG 4 cut(s) 568, 616, 708, 1639
BsmAI GTCTC 6 cut(s) 275, 356, 686, 736, 1190, 1217
BsmBI CGTCTC 1 cut(s) 1217
BsmI GAATGC 1 cut(s) 154
BsnI GGCC 5 cut(s) 527, 561, 1379, 1548, 1643
Bso31I GGTCTC 1 cut(s) 1190
BsoBI CYCGRG 3 cut(s) 203, 1089, 1473
Bsp19I CCATGG 1 cut(s) 1633
BspACI CCGC 5 cut(s) 28, 143, 1206, 1259, 1352
BspANI GGCC 5 cut(s) 527, 561, 1379, 1548, 1643
BspCNI CTCAG 5 cut(s) 361, 646, 748, 916, 1246
BspFNI CGCG 2 cut(s) 838, 1206
BspLI GGNNCC 1 cut(s) 435
BspMI ACCTGC 1 cut(s) 436
BspOI GCTAGC 1 cut(s) 1565
BspPI GGATC 2 cut(s) 251, 1562
BspQI GCTCTTC 2 cut(s) 171, 1405
BspTNI GGTCTC 1 cut(s) 1190
BsrDI GCAATG 2 cut(s) 240, 393
BsrFI RCCGGY 2 cut(s) 17, 1592
BsrI ACTGG 1 cut(s) 1167
BssAI RCCGGY 2 cut(s) 17, 1592
BssECI CCNNGG 4 cut(s) 492, 702, 1472, 1633
BssNAI GTATAC 1 cut(s) 720
BssSI CACGAG 2 cut(s) 987, 1128
BssT1I CCWWGG 2 cut(s) 702, 1633
Bst1107I GTATAC 1 cut(s) 720
Bst2BI CACGAG 2 cut(s) 987, 1128
Bst2UI CCWGG 2 cut(s) 494, 563
Bst4CI ACNGT 5 cut(s) 324, 408, 430, 1103, 1237
Bst6I CTCTTC 4 cut(s) 171, 386, 483, 1405
BstBAI YACGTR 1 cut(s) 1358
BstC8I GCNNGC 3 cut(s) 32, 1077, 1563
BstDEI CTNAG 6 cut(s) 348, 654, 735, 903, 1118, 1254
BstDSI CCRYGG 1 cut(s) 1633
BstENI CCTNNNNNAGG 1 cut(s) 566
BstF5I GGATG 4 cut(s) 195, 466, 1171, 1576
BstFNI CGCG 2 cut(s) 838, 1206
BstHHI GCGC 1 cut(s) 1206
BstMAI GTCTC 6 cut(s) 275, 356, 686, 736, 1190, 1217
BstMCI CGRYCG 1 cut(s) 275
BstMWI GCNNNNNNNGC 5 cut(s) 214, 704, 1349, 1358, 1520
BstNI CCWGG 2 cut(s) 494, 563
BstNSI RCATGY 1 cut(s) 731
BstSCI CCNGG 3 cut(s) 228, 492, 561
BstUI CGCG 2 cut(s) 838, 1206
BstV1I GCAGC 3 cut(s) 1030, 1373, 1402
BstV2I GAAGAC 1 cut(s) 114
BstX2I RGATCY 2 cut(s) 1006, 1567
BstXI CCANNNNNNTGG 1 cut(s) 1691
BstYI RGATCY 2 cut(s) 1006, 1567
BstZ17I GTATAC 1 cut(s) 720
BsuRI GGCC 5 cut(s) 527, 561, 1379, 1548, 1643
BtgI CCRYGG 1 cut(s) 1633
BtgZI GCGATG 1 cut(s) 357
BtsCI GGATG 4 cut(s) 195, 466, 1171, 1576
BtsI GCAGTG 2 cut(s) 579, 1086
BtsIMutI CAGTG 4 cut(s) 579, 651, 1086, 1233
BveI ACCTGC 1 cut(s) 436
Cac8I GCNNGC 3 cut(s) 32, 1077, 1563
CaiI CAGNNNCTG 1 cut(s) 111
CfoI GCGC 1 cut(s) 1206
Cfr10I RCCGGY 2 cut(s) 17, 1592
Cfr13I GGNCC 3 cut(s) 260, 434, 1630
CseI GACGC 2 cut(s) 53, 827
Csp6I GTAC 6 cut(s) 36, 396, 1160, 1213, 1355, 1435
CspAI ACCGGT 2 cut(s) 17, 1592
CspCI CAANNNNNGTGG 4 cut(s) 228, 263, 538, 573
CviAII CATG 8 cut(s) 336, 728, 842, 1063, 1558, 1634, 1664, 1717
CviQI GTAC 6 cut(s) 36, 396, 1160, 1213, 1355, 1435
DdeI CTNAG 6 cut(s) 348, 654, 735, 903, 1118, 1254
Eam1104I CTCTTC 4 cut(s) 171, 386, 483, 1405
EarI CTCTTC 4 cut(s) 171, 386, 483, 1405
EciI GGCGGA 2 cut(s) 158, 1274
Eco130I CCWWGG 2 cut(s) 702, 1633
Eco147I AGGCCT 2 cut(s) 1379, 1548
Eco31I GGTCTC 1 cut(s) 1190
Eco47I GGWCC 3 cut(s) 260, 434, 1630
Eco57I CTGAAG 4 cut(s) 398, 464, 867, 1155
Eco88I CYCGRG 3 cut(s) 203, 1089, 1473
EcoNI CCTNNNNNAGG 1 cut(s) 566
EcoRI GAATTC 1 cut(s) 691
EcoRII CCWGG 2 cut(s) 492, 561
EcoT14I CCWWGG 2 cut(s) 702, 1633
EcoT22I ATGCAT 1 cut(s) 156
ErhI CCWWGG 2 cut(s) 702, 1633
Esp3I CGTCTC 1 cut(s) 1217
FaeI CATG 8 cut(s) 339, 731, 845, 1066, 1561, 1637, 1667, 1720
FatI CATG 8 cut(s) 335, 727, 841, 1062, 1557, 1633, 1663, 1716
FbaI TGATCA 1 cut(s) 1707
FblI GTMKAC 1 cut(s) 719
Fnu4HI GCNGC 3 cut(s) 1044, 1362, 1416
FokI GGATG 4 cut(s) 202, 453, 1178, 1563
Fsp4HI GCNGC 3 cut(s) 1044, 1362, 1416
FspBI CTAG 3 cut(s) 422, 1187, 1562
GlaI GCGC 1 cut(s) 1205
GluI GCNGC 3 cut(s) 1044, 1362, 1416
GsuI CTGGAG 1 cut(s) 665
HaeIII GGCC 5 cut(s) 527, 561, 1379, 1548, 1643
HapII CCGG 3 cut(s) 18, 230, 1593
HgaI GACGC 2 cut(s) 53, 827
HhaI GCGC 1 cut(s) 1206
Hin1II CATG 8 cut(s) 339, 731, 845, 1066, 1561, 1637, 1667, 1720
Hin6I GCGC 1 cut(s) 1204
HinP1I GCGC 1 cut(s) 1204
HinfI GANTC 6 cut(s) 160, 199, 739, 1087, 1334, 1607
HpaII CCGG 3 cut(s) 18, 230, 1593
HphI GGTGA 5 cut(s) 347, 670, 677, 1096, 1384
Hpy166II GTNNAC 3 cut(s) 720, 1051, 1099
Hpy188I TCNGA 4 cut(s) 738, 906, 1255, 1496
Hpy8I GTNNAC 3 cut(s) 720, 1051, 1099
Hpy99I CGWCG 1 cut(s) 142
HpyAV CCTTC 5 cut(s) 219, 295, 373, 574, 1369
HpyCH4III ACNGT 5 cut(s) 324, 408, 430, 1103, 1237
HpyCH4IV ACGT 1 cut(s) 1357
HpyF10VI GCNNNNNNNGC 5 cut(s) 214, 704, 1349, 1358, 1520
HpyF3I CTNAG 6 cut(s) 348, 654, 735, 903, 1118, 1254
HpySE526I ACGT 1 cut(s) 1357
Hsp92II CATG 8 cut(s) 339, 731, 845, 1066, 1561, 1637, 1667, 1720
HspAI GCGC 1 cut(s) 1204
Ksp22I TGATCA 1 cut(s) 1707
LguI GCTCTTC 2 cut(s) 171, 1405
LmnI GCTCC 1 cut(s) 1720
Lsp1109I GCAGC 3 cut(s) 1030, 1373, 1402
LweI GCATC 3 cut(s) 334, 475, 1165
MaeI CTAG 3 cut(s) 422, 1187, 1562
MaeII ACGT 1 cut(s) 1357
MaeIII GTNAC 3 cut(s) 649, 803, 1084
MflI RGATCY 2 cut(s) 1006, 1567
MluI ACGCGT 1 cut(s) 836
MlyI GAGTC 3 cut(s) 154, 1081, 1616
MmeI TCCRAC 2 cut(s) 1219, 1291
Mph1103I ATGCAT 1 cut(s) 156
MroXI GAANNNNTTC 2 cut(s) 82, 852
MseI TTAA 6 cut(s) 14, 762, 938, 1272, 1290, 1617
MslI CAYNNNNRTG 6 cut(s) 656, 864, 948, 1370, 1668, 1703
MspA1I CMGCKG 1 cut(s) 1583
MspI CCGG 3 cut(s) 18, 230, 1593
MspR9I CCNGG 3 cut(s) 230, 494, 563
Mva1269I GAATGC 1 cut(s) 154
MvaI CCWGG 2 cut(s) 494, 563
MvnI CGCG 2 cut(s) 838, 1206
MwoI GCNNNNNNNGC 5 cut(s) 214, 704, 1349, 1358, 1520
NciI CCSGG 1 cut(s) 230
NcoI CCATGG 1 cut(s) 1633
NheI GCTAGC 1 cut(s) 1561
NlaIII CATG 8 cut(s) 339, 731, 845, 1066, 1561, 1637, 1667, 1720
NlaIV GGNNCC 1 cut(s) 435
NmuCI GTSAC 2 cut(s) 649, 1084
NsiI ATGCAT 1 cut(s) 156
NspI RCATGY 1 cut(s) 731
OliI CACNNNNGTG 3 cut(s) 656, 864, 1703
PaeR7I CTCGAG 1 cut(s) 203
PceI AGGCCT 2 cut(s) 1379, 1548
PciSI GCTCTTC 2 cut(s) 171, 1405
PcsI WCGNNNNNNNCGW 1 cut(s) 862
PctI GAATGC 1 cut(s) 154
PdmI GAANNNNTTC 2 cut(s) 82, 852
PfeI GAWTC 3 cut(s) 199, 739, 1334
PinAI ACCGGT 2 cut(s) 17, 1592
PkrI GCNGC 3 cut(s) 1045, 1363, 1417
Ple19I CGATCG 1 cut(s) 275
PleI GAGTC 3 cut(s) 154, 1081, 1615
PpsI GAGTC 3 cut(s) 154, 1081, 1615
Ppu21I YACGTR 1 cut(s) 1358
Psp6I CCWGG 2 cut(s) 492, 561
PspGI CCWGG 2 cut(s) 492, 561
PspN4I GGNNCC 1 cut(s) 435
PspPI GGNCC 3 cut(s) 260, 434, 1630
PstNI CAGNNNCTG 1 cut(s) 111
PsuI RGATCY 2 cut(s) 1006, 1567
PvuI CGATCG 1 cut(s) 275
PvuII CAGCTG 1 cut(s) 1583
RsaI GTAC 6 cut(s) 37, 397, 1161, 1214, 1356, 1436
RsaNI GTAC 6 cut(s) 36, 396, 1160, 1213, 1355, 1435
RseI CAYNNNNRTG 6 cut(s) 656, 864, 948, 1370, 1668, 1703
SapI GCTCTTC 2 cut(s) 171, 1405
SaqAI TTAA 6 cut(s) 14, 762, 938, 1272, 1290, 1617
SatI GCNGC 3 cut(s) 1044, 1362, 1416
Sau96I GGNCC 3 cut(s) 260, 434, 1630
SchI GAGTC 3 cut(s) 154, 1081, 1616
ScrFI CCNGG 3 cut(s) 230, 494, 563
SfaNI GCATC 3 cut(s) 334, 475, 1165
Sfr274I CTCGAG 1 cut(s) 203
SinI GGWCC 3 cut(s) 260, 434, 1630
SlaI CTCGAG 1 cut(s) 203
SmiMI CAYNNNNRTG 6 cut(s) 656, 864, 948, 1370, 1668, 1703
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
SseBI AGGCCT 2 cut(s) 1379, 1548
SsiI CCGC 5 cut(s) 28, 143, 1206, 1259, 1352
SspMI CTAG 3 cut(s) 422, 1187, 1562
StuI AGGCCT 2 cut(s) 1379, 1548
StyD4I CCNGG 3 cut(s) 228, 492, 561
StyI CCWWGG 2 cut(s) 702, 1633
TaaI ACNGT 5 cut(s) 324, 408, 430, 1103, 1237
TaiI ACGT 1 cut(s) 1360
TaqI TCGA 5 cut(s) 158, 197, 204, 813, 1573
TaqII GACCGA 1 cut(s) 1124
TatI WGTACW 2 cut(s) 1159, 1434
TfiI GAWTC 3 cut(s) 199, 739, 1334
Tru1I TTAA 6 cut(s) 14, 762, 938, 1272, 1290, 1617
Tru9I TTAA 6 cut(s) 14, 762, 938, 1272, 1290, 1617
TscAI CASTG 4 cut(s) 579, 658, 1086, 1240
TseFI GTSAC 2 cut(s) 649, 1084
TseI GCWGC 3 cut(s) 1043, 1361, 1415
Tsp45I GTSAC 2 cut(s) 649, 1084
TspDTI ATGAA 4 cut(s) 324, 404, 932, 966
TspRI CASTG 4 cut(s) 579, 658, 1086, 1240
VpaK11BI GGWCC 3 cut(s) 260, 434, 1630
XagI CCTNNNNNAGG 1 cut(s) 566
XapI RAATTY 2 cut(s) 691, 954
XceI RCATGY 1 cut(s) 731
XcmI CCANNNNNNNNNTGG 1 cut(s) 1159
XhoI CTCGAG 1 cut(s) 203
XmiI GTMKAC 1 cut(s) 719
XmnI GAANNNNTTC 2 cut(s) 82, 852
XspI CTAG 3 cut(s) 422, 1187, 1562
Zsp2I ATGCAT 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.