FvH4_6g17480

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001999
Physical Location & Seq
Reverse (-)
11416481 .. 11423480
7000 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g17480.t3

Sequence Viewer

Length: 1446 bp
ATGCTGGGATGTCCGGTGTGGATTGCAGTGGCTCGATTCGATTTCTCGTGGGGCGACCCGGAGTATCACCAGGAGACTTTGGATAATCTCAAGGTGGCTGTGAAGAGTACTAAGAAGCTTTGCGCGGTTATGTTGGACACTGTGGGTCCCGAGTTGCAGGTTGTAAACCGAGGCGAGAAGATCATTGAACTTAAGGGAGATGGCTTGGTTATTCTGACCCCTGATCAGGGACAAGAACCATCTTCAGAGCTTTTGCCCATCAACTTTAATGCATTGTCAAAGGCAGTGACGAAGGGAGATACCGTTTTCATAGGCCAGTACCTGTTCACTGGAAGTGAAACTACTTCTGCATGGTTGGAGGTTTCTGAAGTGAAAGGAGATGATGTTGTATGTGTAGTTAAGAATTCTGCAACACTGGCTGGTTCCTTGTTCACTTTGCATATCTCTCAAGTTCATATCGACTTGCCTACCCTCTCCGATAAAGATAAGCAGGTTATCAGTAGTTGGGGAATGCAAAACAAAATTGATTTCCTCTCACTATCATATACTCGACATGCAGAAGATGTCCGCCAAGCCCGTGAGTATCTTTCTAAGTTGGGTGACCTCAGTCAGACACAAATTTTCGCTAAGATTGAAAATATGGAGGGATTAACCCATTTTGACGAGATTCTACAAGAAGCTGATGGTATTATCCTATCCCGTGGAAATTTGGGCATTGACCTGCCACCTGAGAAGGTGTTTTTATTTCAAAAAGCTGCCCTTTACAAGTGTAACATGGCTGGAAAGCCTGCTGTGGTTACTCGCGTTGTGGATAGTATGACTGACAACTTGAGGCCAACTCGTGCTGAAGCAACTGATGTTGCAAATGCTGTATTAGACGGAAGTGATGCAATTCTTCTTGGTGCCGAGACTCTTCGTGGGTTATACCCTGTTGATACCATCTCTACTGTAGGAAAAATTTGTGCTGAGGCTGAGAAGGTCTTCAACCAAGACTTGTATTTCAAGAGGACTGTCAAATATGTTGGAGAGCCTATGACACACTTGGAATCTATTGCATCCTCAGCGGTTCGAGCAGCCATAAAGGTGAAGGCATCTGTCATTATCTGCTTCACTTCTTCAGGACGGGCTGCAAGATTGATTGCTAAGTACCGACCCACAATGCCAGTTCTTTCAGTCGTCATCCCCCGGCTCAAGACAAATCAGCTGAAGTGGAGCTTTACTGGAGCCTTTGAGGCAAGGCAATCACTCTTAGTCAGGGGTCTTTACCCTATGCTTGCTGATCCTCGACACCCTGCTGAGTCTACCAGTTCAACAAACGAGTCAGTTCTAAAGGTCGCTCTTGATCATGGAAAGGCATCAGGAGTTGTAAAGTCGCACGACCGAGTAGTTGTTTGCCAGAAAGTTGGTGATGCTTCTGTTGTCAAGATTATTGAGCTCGAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

482

Amino Acids

52.58

Weight (kDa)

6.06

Isoelectric Point (pI)

24.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 2 - 332 5.9e-87 Pyruvate kinase, barrel domain
PK_C PF02887 367 - 487 1.3e-23 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 148, 481, 729
AccB1I GGYRCC 1 cut(s) 902
AccI GTMKAC 1 cut(s) 1301
AccII CGCG 2 cut(s) 125, 804
AciI CCGC 3 cut(s) 125, 568, 1064
AclWI GGATC 1 cut(s) 1274
AcsI RAATTY 4 cut(s) 403, 618, 706, 957
AcuI CTGAAG 5 cut(s) 228, 387, 867, 1101, 1226
AfaI GTAC 3 cut(s) 109, 320, 1148
AfiI CCNNNNNNNGG 2 cut(s) 226, 227
AflII CTTAAG 1 cut(s) 191
AgsI TTSAA 6 cut(s) 188, 635, 749, 985, 1003, 1311
AjnI CCWGG 1 cut(s) 69
AluBI AGCT 7 cut(s) 118, 250, 680, 755, 1204, 1215, 1435
AluI AGCT 7 cut(s) 118, 250, 680, 755, 1204, 1215, 1435
Alw21I GWGCWC 1 cut(s) 1437
Alw26I GTCTC 2 cut(s) 68, 902
AlwI GGATC 1 cut(s) 1274
AlwNI CAGNNNCTG 1 cut(s) 322
Ama87I CYCGRG 1 cut(s) 149
AoxI GGCC 2 cut(s) 313, 833
ApeKI GCWGC 3 cut(s) 755, 1073, 1127
ApoI RAATTY 4 cut(s) 403, 618, 706, 957
Asp700I GAANNNNTTC 1 cut(s) 980
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 1 cut(s) 146
AsuC2I CCSGG 2 cut(s) 59, 1186
AsuHPI GGTGA 4 cut(s) 59, 611, 1096, 1418
AvaI CYCGRG 1 cut(s) 149
AvaII GGWCC 1 cut(s) 146
BanI GGYRCC 1 cut(s) 902
BanII GRGCYC 1 cut(s) 1437
BarI GAAGNNNNNNTAC 2 cut(s) 325, 357
BauI CACGAG 2 cut(s) 46, 840
BbsI GAAGAC 1 cut(s) 973
Bbv12I GWGCWC 1 cut(s) 1437
BbvCI CCTCAGC 2 cut(s) 966, 1060
BbvI GCAGC 3 cut(s) 742, 1085, 1114
BccI CCATC 5 cut(s) 194, 247, 266, 677, 947
BciT130I CCWGG 1 cut(s) 71
BclI TGATCA 2 cut(s) 223, 1342
BcnI CCSGG 2 cut(s) 59, 1186
BcoDI GTCTC 2 cut(s) 68, 902
BfmI CTRYAG 1 cut(s) 948
BfrI CTTAAG 1 cut(s) 191
BfuAI ACCTGC 3 cut(s) 148, 481, 729
BglI GCCNNNNNGGC 1 cut(s) 1232
BisI GCNGC 3 cut(s) 756, 1074, 1128
BlsI GCNGC 3 cut(s) 757, 1075, 1129
BmcAI AGTACT 1 cut(s) 109
Bme1390I CCNGG 3 cut(s) 59, 71, 1186
Bme18I GGWCC 1 cut(s) 146
BmeT110I CYCGRG 1 cut(s) 149
BmgT120I GGNCC 1 cut(s) 146
BmiI GGNNCC 5 cut(s) 147, 148, 424, 904, 1225
BmrFI CCNGG 3 cut(s) 59, 71, 1186
BmsI GCATC 5 cut(s) 877, 1064, 1100, 1364, 1399
BoxI GACNNNNGTC 1 cut(s) 606
BpiI GAAGAC 1 cut(s) 973
BplI GAGNNNNNCTC 2 cut(s) 823, 855
BpmI CTGGAG 1 cut(s) 1242
Bpu10I CCTNAGC 2 cut(s) 966, 1060
BpuEI CTTGAG 4 cut(s) 74, 432, 850, 1175
BpuMI CCSGG 2 cut(s) 59, 1186
BsaJI CCNNGG 3 cut(s) 169, 700, 1184
BsaWI WCCGGW 1 cut(s) 13
BsaXI ACNNNNNCTCC 4 cut(s) 288, 318, 369, 399
Bsc4I CCNNNNNNNGG 2 cut(s) 226, 227
Bse1I ACTGG 6 cut(s) 316, 334, 420, 1163, 1225, 1305
BseBI CCWGG 1 cut(s) 71
BseDI CCNNGG 3 cut(s) 169, 700, 1184
BseGI GGATG 3 cut(s) 14, 1055, 1179
BseLI CCNNNNNNNGG 2 cut(s) 226, 227
BseMII CTCAG 6 cut(s) 619, 720, 957, 963, 1074, 1287
BseNI ACTGG 6 cut(s) 316, 334, 420, 1163, 1225, 1305
BseXI GCAGC 3 cut(s) 742, 1085, 1114
BseYI CCCAGC 1 cut(s) 4
Bsh1236I CGCG 2 cut(s) 125, 804
Bsh1285I CGRYCG 1 cut(s) 1381
BshFI GGCC 2 cut(s) 315, 835
BshNI GGYRCC 1 cut(s) 902
BsiEI CGRYCG 1 cut(s) 1381
BsiHKAI GWGCWC 1 cut(s) 1437
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 3 cut(s) 14, 59, 1186
BslFI GGGAC 2 cut(s) 132, 243
BslI CCNNNNNNNGG 2 cut(s) 226, 227
BsmAI GTCTC 2 cut(s) 68, 902
BsmFI GGGAC 2 cut(s) 132, 243
BsmI GAATGC 1 cut(s) 516
BsnI GGCC 2 cut(s) 315, 835
BsoBI CYCGRG 1 cut(s) 149
Bsp1286I GDGCHC 1 cut(s) 1437
Bsp143I GATC 4 cut(s) 180, 223, 1279, 1342
BspACI CCGC 3 cut(s) 125, 568, 1064
BspANI GGCC 2 cut(s) 315, 835
BspCNI CTCAG 6 cut(s) 618, 721, 958, 964, 1073, 1288
BspFNI CGCG 2 cut(s) 125, 804
BspLI GGNNCC 5 cut(s) 147, 148, 424, 904, 1225
BspMI ACCTGC 3 cut(s) 148, 481, 729
BspPI GGATC 1 cut(s) 1274
BspT107I GGYRCC 1 cut(s) 902
BspTI CTTAAG 1 cut(s) 191
BsrI ACTGG 6 cut(s) 316, 334, 420, 1163, 1225, 1305
BssECI CCNNGG 3 cut(s) 169, 700, 1184
BssMI GATC 4 cut(s) 180, 223, 1279, 1342
BssSI CACGAG 2 cut(s) 46, 840
Bst2BI CACGAG 2 cut(s) 46, 840
Bst2UI CCWGG 1 cut(s) 71
Bst4CI ACNGT 4 cut(s) 142, 304, 949, 1012
Bst6I CTCTTC 2 cut(s) 98, 918
BstAFI CTTAAG 1 cut(s) 191
BstC8I GCNNGC 2 cut(s) 789, 1275
BstDSI CCRYGG 1 cut(s) 700
BstEII GGTNACC 1 cut(s) 599
BstF5I GGATG 3 cut(s) 14, 1055, 1179
BstFNI CGCG 2 cut(s) 125, 804
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 4 cut(s) 183, 226, 1282, 1345
BstMAI GTCTC 2 cut(s) 68, 902
BstMBI GATC 4 cut(s) 180, 223, 1279, 1342
BstMCI CGRYCG 1 cut(s) 1381
BstMWI GCNNNNNNNGC 4 cut(s) 416, 1061, 1070, 1232
BstNI CCWGG 1 cut(s) 71
BstNSI RCATGY 1 cut(s) 557
BstPAI GACNNNNGTC 1 cut(s) 606
BstPI GGTNACC 1 cut(s) 599
BstSCI CCNGG 3 cut(s) 57, 69, 1184
BstSFI CTRYAG 1 cut(s) 948
BstUI CGCG 2 cut(s) 125, 804
BstV1I GCAGC 3 cut(s) 742, 1085, 1114
BstV2I GAAGAC 1 cut(s) 973
BstXI CCANNNNNNTGG 1 cut(s) 1403
BsuRI GGCC 2 cut(s) 315, 835
BtgI CCRYGG 1 cut(s) 700
BtsCI GGATG 3 cut(s) 14, 1055, 1179
BtsI GCAGTG 2 cut(s) 33, 291
BtsIMutI CAGTG 5 cut(s) 33, 138, 291, 327, 413
BveI ACCTGC 3 cut(s) 148, 481, 729
Cac8I GCNNGC 2 cut(s) 789, 1275
CaiI CAGNNNCTG 1 cut(s) 322
CfoI GCGC 1 cut(s) 125
Cfr13I GGNCC 1 cut(s) 146
Csp6I GTAC 3 cut(s) 108, 319, 1147
CviAII CATG 4 cut(s) 351, 554, 775, 1346
CviQI GTAC 3 cut(s) 108, 319, 1147
DpnI GATC 4 cut(s) 182, 225, 1281, 1344
DpnII GATC 4 cut(s) 180, 223, 1279, 1342
Eam1104I CTCTTC 2 cut(s) 98, 918
EarI CTCTTC 2 cut(s) 98, 918
EciI GGCGGA 1 cut(s) 557
Ecl136II GAGCTC 1 cut(s) 1435
Eco24I GRGCYC 1 cut(s) 1437
Eco47I GGWCC 1 cut(s) 146
Eco53kI GAGCTC 1 cut(s) 1435
Eco57I CTGAAG 5 cut(s) 228, 387, 867, 1101, 1226
Eco88I CYCGRG 1 cut(s) 149
Eco91I GGTNACC 1 cut(s) 599
EcoICRI GAGCTC 1 cut(s) 1435
EcoO109I RGGNCCY 1 cut(s) 146
EcoO65I GGTNACC 1 cut(s) 599
EcoRI GAATTC 1 cut(s) 403
EcoRII CCWGG 1 cut(s) 69
EcoT22I ATGCAT 1 cut(s) 274
EcoT38I GRGCYC 1 cut(s) 1437
FaeI CATG 4 cut(s) 354, 557, 778, 1349
FalI AAGNNNNNCTT 4 cut(s) 744, 776, 1199, 1231
FaqI GGGAC 2 cut(s) 132, 243
FatI CATG 4 cut(s) 350, 553, 774, 1345
FbaI TGATCA 2 cut(s) 223, 1342
FblI GTMKAC 1 cut(s) 1301
Fnu4HI GCNGC 3 cut(s) 756, 1074, 1128
FokI GGATG 3 cut(s) 21, 1042, 1166
FriOI GRGCYC 1 cut(s) 1437
Fsp4HI GCNGC 3 cut(s) 756, 1074, 1128
GlaI GCGC 1 cut(s) 124
GluI GCNGC 3 cut(s) 756, 1074, 1128
GsaI CCCAGC 1 cut(s) 8
GsuI CTGGAG 1 cut(s) 1242
HaeIII GGCC 2 cut(s) 315, 835
HapII CCGG 3 cut(s) 14, 59, 1186
HhaI GCGC 1 cut(s) 125
Hin1II CATG 4 cut(s) 354, 557, 778, 1349
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HindIII AAGCTT 1 cut(s) 116
HinfI GANTC 6 cut(s) 36, 667, 910, 1046, 1298, 1319
HpaII CCGG 3 cut(s) 14, 59, 1186
HphI GGTGA 4 cut(s) 59, 611, 1096, 1418
Hpy166II GTNNAC 4 cut(s) 166, 327, 432, 1302
Hpy188I TCNGA 5 cut(s) 216, 247, 367, 478, 612
Hpy188III TCNNGA 7 cut(s) 149, 1003, 1119, 1192, 1340, 1359, 1423
Hpy8I GTNNAC 4 cut(s) 166, 327, 432, 1302
HpyAV CCTTC 4 cut(s) 286, 727, 970, 1081
HpyCH4III ACNGT 4 cut(s) 142, 304, 949, 1012
HpyF10VI GCNNNNNNNGC 4 cut(s) 416, 1061, 1070, 1232
Hsp92II CATG 4 cut(s) 354, 557, 778, 1349
HspAI GCGC 1 cut(s) 123
KflI GGGWCCC 1 cut(s) 146
Ksp22I TGATCA 2 cut(s) 223, 1342
Kzo9I GATC 4 cut(s) 180, 223, 1279, 1342
LmnI GCTCC 2 cut(s) 1212, 1223
Lsp1109I GCAGC 3 cut(s) 742, 1085, 1114
LweI GCATC 5 cut(s) 877, 1064, 1100, 1364, 1399
MaeIII GTNAC 4 cut(s) 286, 599, 770, 796
MalI GATC 4 cut(s) 182, 225, 1281, 1344
MboI GATC 4 cut(s) 180, 223, 1279, 1342
MboII GAAGA 8 cut(s) 115, 190, 234, 572, 887, 905, 973, 1107
MhlI GDGCHC 1 cut(s) 1437
MluCI AATT 6 cut(s) 403, 522, 618, 706, 891, 957
MlyI GAGTC 3 cut(s) 904, 1307, 1328
MmeI TCCRAC 3 cut(s) 114, 336, 1003
Mph1103I ATGCAT 1 cut(s) 274
MroXI GAANNNNTTC 1 cut(s) 980
MseI TTAA 4 cut(s) 192, 267, 399, 650
MslI CAYNNNNRTG 1 cut(s) 1082
MspA1I CMGCKG 2 cut(s) 1064, 1204
MspCI CTTAAG 1 cut(s) 191
MspI CCGG 3 cut(s) 14, 59, 1186
MspR9I CCNGG 3 cut(s) 59, 71, 1186
Mva1269I GAATGC 1 cut(s) 516
MvaI CCWGG 1 cut(s) 71
MvnI CGCG 2 cut(s) 125, 804
MwoI GCNNNNNNNGC 4 cut(s) 416, 1061, 1070, 1232
NciI CCSGG 2 cut(s) 59, 1186
NdeII GATC 4 cut(s) 180, 223, 1279, 1342
NlaIII CATG 4 cut(s) 354, 557, 778, 1349
NlaIV GGNNCC 5 cut(s) 147, 148, 424, 904, 1225
NmeAIII GCCGAG 1 cut(s) 931
NmuCI GTSAC 2 cut(s) 286, 599
NsiI ATGCAT 1 cut(s) 274
NspI RCATGY 1 cut(s) 557
PctI GAATGC 1 cut(s) 516
PdmI GAANNNNTTC 1 cut(s) 980
PfeI GAWTC 3 cut(s) 36, 667, 1046
PkrI GCNGC 3 cut(s) 757, 1075, 1129
PleI GAGTC 3 cut(s) 904, 1306, 1327
PpsI GAGTC 3 cut(s) 904, 1306, 1327
PpuMI RGGWCCY 1 cut(s) 146
PshAI GACNNNNGTC 1 cut(s) 606
Psp124BI GAGCTC 1 cut(s) 1437
Psp5II RGGWCCY 1 cut(s) 146
Psp6I CCWGG 1 cut(s) 69
PspEI GGTNACC 1 cut(s) 599
PspFI CCCAGC 1 cut(s) 4
PspGI CCWGG 1 cut(s) 69
PspN4I GGNNCC 5 cut(s) 147, 148, 424, 904, 1225
PspPI GGNCC 1 cut(s) 146
PspPPI RGGWCCY 1 cut(s) 146
PstNI CAGNNNCTG 1 cut(s) 322
PvuII CAGCTG 1 cut(s) 1204
RsaI GTAC 3 cut(s) 109, 320, 1148
RsaNI GTAC 3 cut(s) 108, 319, 1147
RseI CAYNNNNRTG 1 cut(s) 1082
SacI GAGCTC 1 cut(s) 1437
SaqAI TTAA 4 cut(s) 192, 267, 399, 650
SatI GCNGC 3 cut(s) 756, 1074, 1128
Sau3AI GATC 4 cut(s) 180, 223, 1279, 1342
Sau96I GGNCC 1 cut(s) 146
ScaI AGTACT 1 cut(s) 109
SchI GAGTC 3 cut(s) 904, 1307, 1328
ScrFI CCNGG 3 cut(s) 59, 71, 1186
SduI GDGCHC 1 cut(s) 1437
SfaNI GCATC 5 cut(s) 877, 1064, 1100, 1364, 1399
SfcI CTRYAG 1 cut(s) 948
SinI GGWCC 1 cut(s) 146
SmiMI CAYNNNNRTG 1 cut(s) 1082
SmlI CTYRAG 5 cut(s) 89, 191, 447, 829, 1190
SmoI CTYRAG 5 cut(s) 89, 191, 447, 829, 1190
Sse9I AATT 6 cut(s) 403, 522, 618, 706, 891, 957
SsiI CCGC 3 cut(s) 125, 568, 1064
SstI GAGCTC 1 cut(s) 1437
StyD4I CCNGG 3 cut(s) 57, 69, 1184
TaaI ACNGT 4 cut(s) 142, 304, 949, 1012
TaqI TCGA 7 cut(s) 34, 39, 459, 550, 1069, 1285, 1437
TaqII GACCGA 1 cut(s) 1395
TasI AATT 6 cut(s) 403, 522, 618, 706, 891, 957
TatI WGTACW 1 cut(s) 107
TfiI GAWTC 3 cut(s) 36, 667, 1046
Tru1I TTAA 4 cut(s) 192, 267, 399, 650
Tru9I TTAA 4 cut(s) 192, 267, 399, 650
TscAI CASTG 5 cut(s) 33, 145, 291, 334, 420
TseFI GTSAC 2 cut(s) 286, 599
TseI GCWGC 3 cut(s) 755, 1073, 1127
Tsp45I GTSAC 2 cut(s) 286, 599
TspDTI ATGAA 2 cut(s) 298, 443
TspGWI ACGGA 1 cut(s) 894
TspRI CASTG 5 cut(s) 33, 145, 291, 334, 420
Vha464I CTTAAG 1 cut(s) 191
VpaK11BI GGWCC 1 cut(s) 146
XapI RAATTY 4 cut(s) 403, 618, 706, 957
XceI RCATGY 1 cut(s) 557
XmiI GTMKAC 1 cut(s) 1301
XmnI GAANNNNTTC 1 cut(s) 980
ZrmI AGTACT 1 cut(s) 109
Zsp2I ATGCAT 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.