Prupe.7G120700_v2.0.a1

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0001999
Physical Location & Seq
Forward (+)
14410619 .. 14416964
6346 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G120700.2

Sequence Viewer

Length: 1584 bp
ATGCATTCGAATCACCTCCTTCTAGAAGAGCCCATTCGGATGGCTTCGATTCTCGAGCCATCCAAGGCTAGTTTTTTCCCCGCAATGACGAAGATTGTGGGTACGCTCGGGCCCAAATCTCGATCCGTGGAGGTTATCGCTTCTTGCCTCAATGCTGGAATGTCTGTGGCCCGATTCGACTTTTCATGGGGAAACACGGAATATCACCAGGAGACGCTGGAGAATCTGAAGGCGGCTGTGAAGAGTACTAAGAAGCTTTGCGCTGTTATGTTGGACACTGTGGGTCCTGAATTGCAGGTTGCTAACAAAACCGGGAATCCTATTTCACTCCTGGGAGATGGCCTCGTTGTTCTGACACCAGATCGCGGACAGGAACCATCCTCACAGCTGTTGCCCATCAATTTTGATGGATTGTCAAAGGCAGTGAAGAAGGGAGATACCATTTTTGTTGGCCAGTACCTGTTCACTGGAAGTGAAACTACTTCTGTGTGGTTGGAGGTTTCTGAAGTGAAAGGAGAAGATGTTGTTTGTTCAGTAAAGAACTCTGCAACATTGGCTGGTTCATTGTTCACTTTGCATGCCTCTCAAATTCATATTGACCTGCCTACTCTCTCTGCTCAAGATAAGGAAGTTATAAGCAGTTGGGGACTTCAAAACAAAATTGACTTTCTCTCACTGTCATATACCCGGCATGCAGAAGATGTTCGCCAAGCCCGCGAGTTCCTTAATAAGTTGGGTGACCTCAGTCAGACACAGATTTTTGCTAAGATTGAAAATATGGAGGGATTAACCCATTTTGATGAGATTCTACAAGAAGCTGATGGTATTATCCTCTCCCGTGGGAATTTGGGCATTGATCTCCCACCAGAAAAGGTCTTTTTATTTCAGAAGGCTGCCCTTTACAAATGTAACATGGCTGGAAAGCCTGCCGTGGTAACTCGTGTTGTGGATAGTATGACTGACAACTTGAGGCCAACCCGTGCAGAAGCAACTGATGTTGCAAATGCTGTATTGGATGGAAGTGATGCAATTCTTCTTGGTGCCGAGACTCTTCGTGGTTTGTACCCTGTTGATACTATCTCTACGGTTGGTAGAATTTGTGCCGAGGCAGAGAAGGTTTTCAACCAAGACTTATATTTCAAAAAGACTGTCAAATATGTTGGAGAGCCAATGACACACTTGGAATCTATTGCATCCTCAGCGGTACGGGCAGCCATTAAGGTGAAGGCATCTGTTATTATTTGCTTCACTTCTTCTGGAAGGGCTGCAAGATTGATTGCTAAGTATCGGCCCACGATGCCCGTTCTTTCAGTCGTCGTTCCTCGGCTCAAGACTAATCAACTTAAATGGAGCTTTACTGGAGCCTTTGAGGCAAGGCAATCGCTTTTAGTCAGGGGTCTTTACCCTATGCTTGCTGATCCTCGACATCCTGCTGAGTCTACCAGTGCAACAAACGAGTCAGTTCTGAAGGTTGCTCTTGATCACGGAAAAGCTTCCGGAGTGGTAAAGTCGCACGACCGAGTAGTTGTTTGCCAGAAAGTTGGTGATGCCTCTGTTGTCAAGATTATAGAGCTTGAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

528

Amino Acids

57.26

Weight (kDa)

6.62

Isoelectric Point (pI)

29.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 635
Acc36I ACCTGC 2 cut(s) 286, 609
AccB1I GGYRCC 1 cut(s) 1040
AccI GTMKAC 1 cut(s) 1439
AccII CGCG 2 cut(s) 366, 717
AccIII TCCGGA 1 cut(s) 1496
AciI CCGC 5 cut(s) 81, 233, 366, 715, 1202
AclWI GGATC 2 cut(s) 117, 1412
AcoI YGGCCR 1 cut(s) 451
AcsI RAATTY 3 cut(s) 588, 844, 1095
AcuI CTGAAG 3 cut(s) 248, 525, 1487
AfaI GTAC 5 cut(s) 103, 247, 458, 1064, 1206
AfiI CCNNNNNNNGG 1 cut(s) 365
AgsI TTSAA 5 cut(s) 653, 773, 1123, 1141, 1577
AjnI CCWGG 2 cut(s) 207, 330
AluBI AGCT 6 cut(s) 256, 388, 818, 1353, 1493, 1573
AluI AGCT 6 cut(s) 256, 388, 818, 1353, 1493, 1573
Alw26I GTCTC 2 cut(s) 206, 1040
AlwI GGATC 2 cut(s) 117, 1412
AlwNI CAGNNNCTG 1 cut(s) 460
Ama87I CYCGRG 2 cut(s) 53, 107
Aor13HI TCCGGA 1 cut(s) 1496
AoxI GGCC 6 cut(s) 110, 168, 340, 451, 971, 1289
ApaI GGGCCC 1 cut(s) 114
ApeKI GCWGC 3 cut(s) 893, 1211, 1265
ApoI RAATTY 3 cut(s) 588, 844, 1095
ArsI GACNNNNNNTTYG 2 cut(s) 1134, 1166
Asp700I GAANNNNTTC 3 cut(s) 702, 1118, 1492
AspLEI GCGC 1 cut(s) 263
AspS9I GGNCC 5 cut(s) 110, 111, 169, 284, 1290
AsuC2I CCSGG 2 cut(s) 313, 688
AsuHPI GGTGA 5 cut(s) 5, 197, 749, 1234, 1556
AsuII TTCGAA 1 cut(s) 8
AvaI CYCGRG 2 cut(s) 53, 107
AvaII GGWCC 1 cut(s) 284
BaeGI GKGCMC 1 cut(s) 114
BalI TGGCCA 1 cut(s) 453
BanI GGYRCC 1 cut(s) 1040
BanII GRGCYC 2 cut(s) 33, 114
BarI GAAGNNNNNNTAC 2 cut(s) 463, 495
BauI CACGAG 1 cut(s) 939
BbvCI CCTCAGC 1 cut(s) 1198
BbvI GCAGC 3 cut(s) 880, 1223, 1252
BccI CCATC 8 cut(s) 34, 67, 332, 385, 401, 404, 815, 1010
BceAI ACGGC 1 cut(s) 914
BcgI CGANNNNNNTGC 2 cut(s) 1362, 1396
BciT130I CCWGG 2 cut(s) 209, 332
BclI TGATCA 1 cut(s) 1480
BcnI CCSGG 2 cut(s) 313, 688
BcoDI GTCTC 2 cut(s) 206, 1040
BfaI CTAG 2 cut(s) 23, 69
BfuAI ACCTGC 2 cut(s) 286, 609
BglI GCCNNNNNGGC 1 cut(s) 1370
BisI GCNGC 4 cut(s) 234, 894, 1212, 1266
BlsI GCNGC 4 cut(s) 235, 895, 1213, 1267
BmcAI AGTACT 1 cut(s) 247
Bme1390I CCNGG 4 cut(s) 209, 313, 332, 688
Bme18I GGWCC 1 cut(s) 284
BmeT110I CYCGRG 2 cut(s) 53, 107
BmgT120I GGNCC 5 cut(s) 110, 111, 169, 284, 1290
BmiI GGNNCC 5 cut(s) 112, 285, 375, 1042, 1363
BmrFI CCNGG 4 cut(s) 209, 313, 332, 688
BmsI GCATC 5 cut(s) 1015, 1202, 1238, 1287, 1537
BoxI GACNNNNGTC 1 cut(s) 744
BplI GAGNNNNNCTC 2 cut(s) 327, 359
BpmI CTGGAG 2 cut(s) 239, 1380
Bpu10I CCTNAGC 1 cut(s) 1198
Bpu14I TTCGAA 1 cut(s) 8
BpuEI CTTGAG 3 cut(s) 603, 988, 1313
BpuMI CCSGG 2 cut(s) 313, 688
BsaJI CCNNGG 7 cut(s) 63, 126, 331, 838, 930, 1104, 1322
BsaWI WCCGGW 1 cut(s) 1496
BsaXI ACNNNNNCTCC 2 cut(s) 507, 537
Bsc4I CCNNNNNNNGG 1 cut(s) 365
Bse1I ACTGG 4 cut(s) 454, 472, 1363, 1443
Bse3DI GCAATG 1 cut(s) 90
BseAI TCCGGA 1 cut(s) 1496
BseBI CCWGG 2 cut(s) 209, 332
BseDI CCNNGG 7 cut(s) 63, 126, 331, 838, 930, 1104, 1322
BseGI GGATG 6 cut(s) 45, 59, 377, 1021, 1193, 1426
BseLI CCNNNNNNNGG 1 cut(s) 365
BseMI GCAATG 1 cut(s) 90
BseMII CTCAG 3 cut(s) 757, 1212, 1425
BseNI ACTGG 4 cut(s) 454, 472, 1363, 1443
BseSI GKGCMC 1 cut(s) 114
BseXI GCAGC 3 cut(s) 880, 1223, 1252
BsgI GTGCAG 1 cut(s) 1002
Bsh1236I CGCG 2 cut(s) 366, 717
Bsh1285I CGRYCG 1 cut(s) 1519
BshFI GGCC 6 cut(s) 112, 170, 342, 453, 973, 1291
BshNI GGYRCC 1 cut(s) 1040
BsiEI CGRYCG 1 cut(s) 1519
BsiHKCI CYCGRG 2 cut(s) 53, 107
BsiSI CCGG 3 cut(s) 312, 688, 1497
BslFI GGGAC 1 cut(s) 660
BslI CCNNNNNNNGG 1 cut(s) 365
BsmAI GTCTC 2 cut(s) 206, 1040
BsmBI CGTCTC 1 cut(s) 206
BsmFI GGGAC 1 cut(s) 660
BsmI GAATGC 1 cut(s) 4
BsnI GGCC 6 cut(s) 112, 170, 342, 453, 973, 1291
BsoBI CYCGRG 2 cut(s) 53, 107
Bsp119I TTCGAA 1 cut(s) 8
Bsp120I GGGCCC 1 cut(s) 110
Bsp1286I GDGCHC 2 cut(s) 33, 114
Bsp13I TCCGGA 1 cut(s) 1496
Bsp143I GATC 5 cut(s) 122, 361, 856, 1417, 1480
BspACI CCGC 5 cut(s) 81, 233, 366, 715, 1202
BspANI GGCC 6 cut(s) 112, 170, 342, 453, 973, 1291
BspCNI CTCAG 3 cut(s) 756, 1211, 1426
BspEI TCCGGA 1 cut(s) 1496
BspFNI CGCG 2 cut(s) 366, 717
BspLI GGNNCC 5 cut(s) 112, 285, 375, 1042, 1363
BspMI ACCTGC 2 cut(s) 286, 609
BspPI GGATC 2 cut(s) 117, 1412
BspQI GCTCTTC 1 cut(s) 21
BspT104I TTCGAA 1 cut(s) 8
BspT107I GGYRCC 1 cut(s) 1040
BsrDI GCAATG 1 cut(s) 90
BsrI ACTGG 4 cut(s) 454, 472, 1363, 1443
BssECI CCNNGG 7 cut(s) 63, 126, 331, 838, 930, 1104, 1322
BssMI GATC 5 cut(s) 122, 361, 856, 1417, 1480
BssSI CACGAG 1 cut(s) 939
BssT1I CCWWGG 1 cut(s) 63
Bst2BI CACGAG 1 cut(s) 939
Bst2UI CCWGG 2 cut(s) 209, 332
Bst4CI ACNGT 4 cut(s) 280, 678, 1087, 1150
Bst6I CTCTTC 3 cut(s) 21, 236, 1056
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 5 cut(s) 579, 693, 715, 927, 1413
BstDEI CTNAG 6 cut(s) 249, 743, 765, 1198, 1281, 1434
BstDSI CCRYGG 3 cut(s) 126, 838, 930
BstEII GGTNACC 1 cut(s) 737
BstF5I GGATG 6 cut(s) 45, 59, 377, 1021, 1193, 1426
BstFNI CGCG 2 cut(s) 366, 717
BstHHI GCGC 1 cut(s) 263
BstKTI GATC 5 cut(s) 125, 364, 859, 1420, 1483
BstMAI GTCTC 2 cut(s) 206, 1040
BstMBI GATC 5 cut(s) 122, 361, 856, 1417, 1480
BstMCI CGRYCG 1 cut(s) 1519
BstMWI GCNNNNNNNGC 6 cut(s) 554, 714, 1199, 1208, 1297, 1370
BstNI CCWGG 2 cut(s) 209, 332
BstNSI RCATGY 2 cut(s) 581, 695
BstPAI GACNNNNGTC 1 cut(s) 744
BstPI GGTNACC 1 cut(s) 737
BstSCI CCNGG 4 cut(s) 207, 311, 330, 686
BstSLI GKGCMC 1 cut(s) 114
BstUI CGCG 2 cut(s) 366, 717
BstV1I GCAGC 3 cut(s) 880, 1223, 1252
BstXI CCANNNNNNTGG 2 cut(s) 40, 1541
BsuRI GGCC 6 cut(s) 112, 170, 342, 453, 973, 1291
BtgI CCRYGG 3 cut(s) 126, 838, 930
BtsCI GGATG 6 cut(s) 45, 59, 377, 1021, 1193, 1426
BtsI GCAGTG 1 cut(s) 429
BtsIMutI CAGTG 5 cut(s) 276, 429, 465, 674, 1450
BveI ACCTGC 2 cut(s) 286, 609
Cac8I GCNNGC 5 cut(s) 579, 693, 715, 927, 1413
CaiI CAGNNNCTG 1 cut(s) 460
CfoI GCGC 1 cut(s) 263
Cfr13I GGNCC 5 cut(s) 110, 111, 169, 284, 1290
CseI GACGC 1 cut(s) 223
Csp6I GTAC 5 cut(s) 102, 246, 457, 1063, 1205
CviAII CATG 4 cut(s) 186, 578, 692, 913
CviQI GTAC 5 cut(s) 102, 246, 457, 1063, 1205
DdeI CTNAG 6 cut(s) 249, 743, 765, 1198, 1281, 1434
DpnI GATC 5 cut(s) 124, 363, 858, 1419, 1482
DpnII GATC 5 cut(s) 122, 361, 856, 1417, 1480
EaeI YGGCCR 1 cut(s) 451
Eam1104I CTCTTC 3 cut(s) 21, 236, 1056
EarI CTCTTC 3 cut(s) 21, 236, 1056
Eco130I CCWWGG 1 cut(s) 63
Eco24I GRGCYC 2 cut(s) 33, 114
Eco47I GGWCC 1 cut(s) 284
Eco57I CTGAAG 3 cut(s) 248, 525, 1487
Eco88I CYCGRG 2 cut(s) 53, 107
Eco91I GGTNACC 1 cut(s) 737
EcoO109I RGGNCCY 1 cut(s) 284
EcoO65I GGTNACC 1 cut(s) 737
EcoRII CCWGG 2 cut(s) 207, 330
EcoT14I CCWWGG 1 cut(s) 63
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 33, 114
ErhI CCWWGG 1 cut(s) 63
Esp3I CGTCTC 1 cut(s) 206
FaeI CATG 4 cut(s) 189, 581, 695, 916
FaqI GGGAC 1 cut(s) 660
FatI CATG 4 cut(s) 185, 577, 691, 912
FauI CCCGC 2 cut(s) 88, 722
FbaI TGATCA 1 cut(s) 1480
FblI GTMKAC 1 cut(s) 1439
Fnu4HI GCNGC 4 cut(s) 234, 894, 1212, 1266
FokI GGATG 6 cut(s) 46, 52, 364, 1028, 1180, 1413
FriOI GRGCYC 2 cut(s) 33, 114
Fsp4HI GCNGC 4 cut(s) 234, 894, 1212, 1266
FspBI CTAG 2 cut(s) 23, 69
GlaI GCGC 1 cut(s) 262
GluI GCNGC 4 cut(s) 234, 894, 1212, 1266
GsuI CTGGAG 2 cut(s) 239, 1380
HaeIII GGCC 6 cut(s) 112, 170, 342, 453, 973, 1291
HapII CCGG 3 cut(s) 312, 688, 1497
HgaI GACGC 1 cut(s) 223
HhaI GCGC 1 cut(s) 263
Hin1II CATG 4 cut(s) 189, 581, 695, 916
Hin6I GCGC 1 cut(s) 261
HinP1I GCGC 1 cut(s) 261
HindIII AAGCTT 2 cut(s) 254, 1491
HpaII CCGG 3 cut(s) 312, 688, 1497
HphI GGTGA 5 cut(s) 5, 197, 749, 1234, 1556
Hpy166II GTNNAC 3 cut(s) 465, 570, 1440
Hpy188I TCNGA 7 cut(s) 39, 228, 354, 505, 750, 888, 1467
Hpy8I GTNNAC 3 cut(s) 465, 570, 1440
Hpy99I CGWCG 1 cut(s) 1319
HpyAV CCTTC 8 cut(s) 29, 223, 424, 883, 1108, 1219, 1254, 1462
HpyCH4III ACNGT 4 cut(s) 280, 678, 1087, 1150
HpyF10VI GCNNNNNNNGC 6 cut(s) 554, 714, 1199, 1208, 1297, 1370
HpyF3I CTNAG 6 cut(s) 249, 743, 765, 1198, 1281, 1434
Hsp92II CATG 4 cut(s) 189, 581, 695, 916
HspAI GCGC 1 cut(s) 261
Kpn2I TCCGGA 1 cut(s) 1496
Ksp22I TGATCA 1 cut(s) 1480
Kzo9I GATC 5 cut(s) 122, 361, 856, 1417, 1480
LguI GCTCTTC 1 cut(s) 21
LmnI GCTCC 2 cut(s) 1350, 1361
Lsp1109I GCAGC 3 cut(s) 880, 1223, 1252
LweI GCATC 5 cut(s) 1015, 1202, 1238, 1287, 1537
MaeI CTAG 2 cut(s) 23, 69
MaeIII GTNAC 3 cut(s) 737, 908, 934
MalI GATC 5 cut(s) 124, 363, 858, 1419, 1482
MboI GATC 5 cut(s) 122, 361, 856, 1417, 1480
MboII GAAGA 9 cut(s) 38, 103, 253, 439, 530, 710, 1025, 1043, 1245
MhlI GDGCHC 2 cut(s) 33, 114
MlsI TGGCCA 1 cut(s) 453
MluCI AATT 7 cut(s) 290, 400, 588, 660, 844, 1029, 1095
MluNI TGGCCA 1 cut(s) 453
MlyI GAGTC 3 cut(s) 1042, 1445, 1466
MmeI TCCRAC 3 cut(s) 252, 474, 1141
Mox20I TGGCCA 1 cut(s) 453
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 1496
MroXI GAANNNNTTC 3 cut(s) 702, 1118, 1492
MscI TGGCCA 1 cut(s) 453
MseI TTAA 4 cut(s) 726, 788, 1218, 1344
MslI CAYNNNNRTG 3 cut(s) 38, 798, 1220
Msp20I TGGCCA 1 cut(s) 453
MspA1I CMGCKG 2 cut(s) 388, 1202
MspI CCGG 3 cut(s) 312, 688, 1497
MspR9I CCNGG 4 cut(s) 209, 313, 332, 688
Mva1269I GAATGC 1 cut(s) 4
MvaI CCWGG 2 cut(s) 209, 332
MvnI CGCG 2 cut(s) 366, 717
MwoI GCNNNNNNNGC 6 cut(s) 554, 714, 1199, 1208, 1297, 1370
NciI CCSGG 2 cut(s) 313, 688
NdeII GATC 5 cut(s) 122, 361, 856, 1417, 1480
NlaIII CATG 4 cut(s) 189, 581, 695, 916
NlaIV GGNNCC 5 cut(s) 112, 285, 375, 1042, 1363
NmeAIII GCCGAG 3 cut(s) 1069, 1129, 1303
NmuCI GTSAC 1 cut(s) 737
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 581, 695
NspV TTCGAA 1 cut(s) 8
PaeI GCATGC 2 cut(s) 581, 695
PaeR7I CTCGAG 1 cut(s) 53
PciSI GCTCTTC 1 cut(s) 21
PctI GAATGC 1 cut(s) 4
PdmI GAANNNNTTC 3 cut(s) 702, 1118, 1492
PfeI GAWTC 7 cut(s) 10, 49, 174, 223, 316, 805, 1184
PkrI GCNGC 4 cut(s) 235, 895, 1213, 1267
PleI GAGTC 3 cut(s) 1042, 1444, 1465
PpsI GAGTC 3 cut(s) 1042, 1444, 1465
PpuMI RGGWCCY 1 cut(s) 284
PshAI GACNNNNGTC 1 cut(s) 744
PsiI TTATAA 1 cut(s) 635
Psp5II RGGWCCY 1 cut(s) 284
Psp6I CCWGG 2 cut(s) 207, 330
PspEI GGTNACC 1 cut(s) 737
PspGI CCWGG 2 cut(s) 207, 330
PspN4I GGNNCC 5 cut(s) 112, 285, 375, 1042, 1363
PspOMI GGGCCC 1 cut(s) 110
PspPI GGNCC 5 cut(s) 110, 111, 169, 284, 1290
PspPPI RGGWCCY 1 cut(s) 284
PstNI CAGNNNCTG 1 cut(s) 460
PvuII CAGCTG 1 cut(s) 388
RsaI GTAC 5 cut(s) 103, 247, 458, 1064, 1206
RsaNI GTAC 5 cut(s) 102, 246, 457, 1063, 1205
RseI CAYNNNNRTG 3 cut(s) 38, 798, 1220
SapI GCTCTTC 1 cut(s) 21
SaqAI TTAA 4 cut(s) 726, 788, 1218, 1344
SatI GCNGC 4 cut(s) 234, 894, 1212, 1266
Sau3AI GATC 5 cut(s) 122, 361, 856, 1417, 1480
Sau96I GGNCC 5 cut(s) 110, 111, 169, 284, 1290
ScaI AGTACT 1 cut(s) 247
SchI GAGTC 3 cut(s) 1042, 1445, 1466
ScrFI CCNGG 4 cut(s) 209, 313, 332, 688
SduI GDGCHC 2 cut(s) 33, 114
SfaNI GCATC 5 cut(s) 1015, 1202, 1238, 1287, 1537
Sfr274I CTCGAG 1 cut(s) 53
SfuI TTCGAA 1 cut(s) 8
SinI GGWCC 1 cut(s) 284
SlaI CTCGAG 1 cut(s) 53
SmiMI CAYNNNNRTG 3 cut(s) 38, 798, 1220
SmlI CTYRAG 4 cut(s) 53, 618, 967, 1328
SmoI CTYRAG 4 cut(s) 53, 618, 967, 1328
SphI GCATGC 2 cut(s) 581, 695
Sse9I AATT 7 cut(s) 290, 400, 588, 660, 844, 1029, 1095
SsiI CCGC 5 cut(s) 81, 233, 366, 715, 1202
SspMI CTAG 2 cut(s) 23, 69
StyD4I CCNGG 4 cut(s) 207, 311, 330, 686
StyI CCWWGG 1 cut(s) 63
TaaI ACNGT 4 cut(s) 280, 678, 1087, 1150
TaqI TCGA 6 cut(s) 8, 47, 54, 121, 177, 1423
TaqII GACCGA 1 cut(s) 1533
TasI AATT 7 cut(s) 290, 400, 588, 660, 844, 1029, 1095
TatI WGTACW 1 cut(s) 245
TauI GCSGC 1 cut(s) 236
TfiI GAWTC 7 cut(s) 10, 49, 174, 223, 316, 805, 1184
Tru1I TTAA 4 cut(s) 726, 788, 1218, 1344
Tru9I TTAA 4 cut(s) 726, 788, 1218, 1344
TscAI CASTG 5 cut(s) 283, 429, 472, 681, 1450
TseFI GTSAC 1 cut(s) 737
TseI GCWGC 3 cut(s) 893, 1211, 1265
Tsp45I GTSAC 1 cut(s) 737
TspDTI ATGAA 3 cut(s) 174, 552, 581
TspGWI ACGGA 3 cut(s) 115, 212, 1500
TspRI CASTG 5 cut(s) 283, 429, 472, 681, 1450
VpaK11BI GGWCC 1 cut(s) 284
XapI RAATTY 3 cut(s) 588, 844, 1095
XbaI TCTAGA 1 cut(s) 22
XceI RCATGY 2 cut(s) 581, 695
XhoI CTCGAG 1 cut(s) 53
XmiI GTMKAC 1 cut(s) 1439
XmnI GAANNNNTTC 3 cut(s) 702, 1118, 1492
XspI CTAG 2 cut(s) 23, 69
ZrmI AGTACT 1 cut(s) 247
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.