RLG00000024139

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001999
Physical Location & Seq
Forward (+)
32382313 .. 32387979
5667 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024139

Sequence Viewer

Length: 1584 bp
ATGCATTCGAATCATCTGCTTCTGGAGGAGCCGATAAGGATGGCTTCGATACTGGAGCCGTCGAAGGCGAGTTTTTTTCCTGCAATGACGAAGATTGTGGGGACGCTGGGGCCGAAATCCCGATCTGTGGAGGTCATCTCTTCTTGCCTGAATGCTGGAATGTCTGTGGCCCGATTCGATTTCTCGTGGGGCAACCCGGAGTATCACCAGGAGACTTTGGACAATCTCAAGGCGGCTGTTAAGTGTACCAAGAAGCTTTGCGCGGTTATGTTGGACACTGTGGGTCCCGAATTGCAGGTTGTAAACAGAGGCGAGAAAATCATTGAACTCAAGGGAGATGGCTTGGTTATTCTTACCCCTGATCAGGGACAAGAACCATCTTCGGAGCTTTTGCCCATCAATTTTGATGGATTGTCAAAGGCAGTGACAAAGGGAGATACCGTTTTCATAGGCCAGTACCTGTTCACTGGAAGTGAAACTACTTCTGCATGGTTGGAGGTGTCTGAAGTGAAAGGAGATGATGTCGTTTGTGTAGTAAAGAATTCTGCAACATTGGCTGGTTCTTTGTTCACTTTGCATATCTCTCAAGTTCATATTGACTTGCCTACTCTCTCTGATAAAGATAAGCAGGTTATTAGTAGTTGGGGACTTAAAAACAAAATTGATTTCCTCTCACTGTCATATACACGACATGCAGAAGATGTTCGCCAAGCCCGCGAGTACCTTTCTAAGTTGGGTGACCTCAGTCAGACACAAATTTTTGCAAAAATTGAAAATATGGAGGGATTAACCCATTTTGACGAGATTCTACAAGAAGCTGACGGTATTATCCTATCCCGTGGAAATTTGGGCATTGACCTGCCACCTGAGAAGGTGTTTTTATTTCAAAAGGCTGCTCTTTACAAGTGTAACATGGCTGGAAAGCCTGCTGTGGTTACTCGTGTTGTGGATAGTATGACCGACAACTTGAGGCCAACTCGTGCAGAAGCAACTGATGTTGCAAATGCTGTATTAGACGGAAGTGATGCAATTCTTCTCGGTGCTGAGACTCTTCGTGGGTTATACCCTGTTGATACCATCTCTACTGTTGGCAAAATTTGTGCTGAGGCAGAGAAGGTCTTCAACCAGGACTTGTATTTCAAGAGGACAGTCAAATACGTTGGAGAGCCAATGACACACTTGGAATCTATTGCATCATCTGCGGTTCGGGCAGCCATAAAGGTGAAGGCATCTGTTATTATTTGCTTCACTTCTTCAGGACGGGCTGCAAGATTGATTGCTAAGTACCGACCCACAATGCCAGTTCTTTCAGTCGTCATACCCCGGCTCAAGACAAATCAGCTTAAGTGGAGCTTTACTGGAGCCTTTGAGGCAAGGCAATCACTCTTAGTCAGGGGTCTCTACCCTATGCTTGCTGATCCTCGACACCCTGCTGAGTCCACCAGTTCAACAAACGAGTCGGTTCTAAAGGTTGCTCTTGATCATGGAAAGGCATCAGGAGTTGTAAAGTCGCACGACCGAGTAGTTGTTTGCCAGAAAGTGGGTGATGCCTCTGTTGTCAAGATTATTGAGCTCGAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

528

Amino Acids

57.46

Weight (kDa)

6.45

Isoelectric Point (pI)

30.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 30 - 360 4.3e-87 Pyruvate kinase, barrel domain
PK_C PF02887 395 - 515 1.4e-23 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 286, 619, 867
AccB7I CCANNNNNTGG 1 cut(s) 1540
AccII CGCG 2 cut(s) 263, 717
AciI CCGC 4 cut(s) 233, 263, 715, 1202
AclWI GGATC 1 cut(s) 1412
AcsI RAATTY 4 cut(s) 541, 756, 844, 1095
AcuI CTGAAG 2 cut(s) 525, 1239
AfaI GTAC 4 cut(s) 247, 458, 722, 1286
AfiI CCNNNNNNNGG 4 cut(s) 127, 364, 365, 1540
AflII CTTAAG 1 cut(s) 1343
AgsI TTSAA 6 cut(s) 326, 773, 887, 1123, 1141, 1449
AjnI CCWGG 2 cut(s) 207, 1125
AluBI AGCT 6 cut(s) 256, 388, 818, 1342, 1353, 1573
AluI AGCT 6 cut(s) 256, 388, 818, 1342, 1353, 1573
Alw21I GWGCWC 1 cut(s) 1575
Alw26I GTCTC 3 cut(s) 206, 1040, 1403
AlwI GGATC 1 cut(s) 1412
AlwNI CAGNNNCTG 1 cut(s) 460
AoxI GGCC 4 cut(s) 110, 168, 451, 971
ApeKI GCWGC 3 cut(s) 893, 1211, 1265
ApoI RAATTY 4 cut(s) 541, 756, 844, 1095
Asp700I GAANNNNTTC 2 cut(s) 702, 1118
AspLEI GCGC 1 cut(s) 263
AspS9I GGNCC 3 cut(s) 110, 169, 284
AsuC2I CCSGG 2 cut(s) 197, 1324
AsuHPI GGTGA 4 cut(s) 197, 749, 1234, 1556
AsuII TTCGAA 1 cut(s) 8
AvaII GGWCC 1 cut(s) 284
BanII GRGCYC 1 cut(s) 1575
BarI GAAGNNNNNNTAC 2 cut(s) 463, 495
BauI CACGAG 3 cut(s) 184, 939, 978
BbsI GAAGAC 1 cut(s) 1111
Bbv12I GWGCWC 1 cut(s) 1575
BbvCI CCTCAGC 1 cut(s) 1104
BbvI GCAGC 3 cut(s) 880, 1223, 1252
BccI CCATC 6 cut(s) 34, 332, 385, 401, 404, 1085
BceAI ACGGC 1 cut(s) 43
BcgI CGANNNNNNTGC 1 cut(s) 32
BciT130I CCWGG 2 cut(s) 209, 1127
BclI TGATCA 2 cut(s) 361, 1480
BcnI CCSGG 2 cut(s) 197, 1324
BcoDI GTCTC 3 cut(s) 206, 1040, 1403
BfrI CTTAAG 1 cut(s) 1343
BfuAI ACCTGC 3 cut(s) 286, 619, 867
BglI GCCNNNNNGGC 1 cut(s) 1370
BisI GCNGC 4 cut(s) 234, 894, 1212, 1266
BlsI GCNGC 4 cut(s) 235, 895, 1213, 1267
Bme1390I CCNGG 4 cut(s) 197, 209, 1127, 1324
Bme18I GGWCC 1 cut(s) 284
BmgT120I GGNCC 3 cut(s) 110, 169, 284
BmiI GGNNCC 6 cut(s) 30, 57, 111, 285, 286, 1363
BmrFI CCNGG 4 cut(s) 197, 209, 1127, 1324
BmsI GCATC 5 cut(s) 1015, 1202, 1238, 1502, 1537
BoxI GACNNNNGTC 1 cut(s) 744
BpiI GAAGAC 1 cut(s) 1111
BplI GAGNNNNNCTC 4 cut(s) 122, 154, 961, 993
BpmI CTGGAG 3 cut(s) 44, 74, 1380
Bpu10I CCTNAGC 1 cut(s) 1104
Bpu14I TTCGAA 1 cut(s) 8
BpuEI CTTGAG 5 cut(s) 212, 314, 570, 988, 1313
BpuMI CCSGG 2 cut(s) 197, 1324
BsaI GGTCTC 1 cut(s) 1403
BsaJI CCNNGG 2 cut(s) 838, 1322
BsaXI ACNNNNNCTCC 4 cut(s) 426, 456, 507, 537
Bsc4I CCNNNNNNNGG 4 cut(s) 127, 364, 365, 1540
Bse1I ACTGG 6 cut(s) 57, 454, 472, 1301, 1363, 1443
Bse3DI GCAATG 1 cut(s) 90
BseBI CCWGG 2 cut(s) 209, 1127
BseDI CCNNGG 2 cut(s) 838, 1322
BseGI GGATG 1 cut(s) 45
BseLI CCNNNNNNNGG 4 cut(s) 127, 364, 365, 1540
BseMI GCAATG 1 cut(s) 90
BseMII CTCAG 5 cut(s) 757, 858, 1035, 1095, 1425
BseNI ACTGG 6 cut(s) 57, 454, 472, 1301, 1363, 1443
BseRI GAGGAG 1 cut(s) 41
BseXI GCAGC 3 cut(s) 880, 1223, 1252
BseYI CCCAGC 1 cut(s) 106
BsgI GTGCAG 1 cut(s) 1002
Bsh1236I CGCG 2 cut(s) 263, 717
Bsh1285I CGRYCG 1 cut(s) 1519
BshFI GGCC 4 cut(s) 112, 170, 453, 973
BsiEI CGRYCG 1 cut(s) 1519
BsiHKAI GWGCWC 1 cut(s) 1575
BsiSI CCGG 2 cut(s) 197, 1324
BslFI GGGAC 4 cut(s) 115, 270, 381, 660
BslI CCNNNNNNNGG 4 cut(s) 127, 364, 365, 1540
BsmAI GTCTC 3 cut(s) 206, 1040, 1403
BsmFI GGGAC 4 cut(s) 115, 270, 381, 660
BsmI GAATGC 2 cut(s) 4, 157
BsnI GGCC 4 cut(s) 112, 170, 453, 973
Bso31I GGTCTC 1 cut(s) 1403
Bsp119I TTCGAA 1 cut(s) 8
Bsp1286I GDGCHC 1 cut(s) 1575
Bsp143I GATC 4 cut(s) 122, 361, 1417, 1480
BspACI CCGC 4 cut(s) 233, 263, 715, 1202
BspANI GGCC 4 cut(s) 112, 170, 453, 973
BspCNI CTCAG 5 cut(s) 756, 859, 1036, 1096, 1426
BspFNI CGCG 2 cut(s) 263, 717
BspLI GGNNCC 6 cut(s) 30, 57, 111, 285, 286, 1363
BspMI ACCTGC 3 cut(s) 286, 619, 867
BspPI GGATC 1 cut(s) 1412
BspT104I TTCGAA 1 cut(s) 8
BspTI CTTAAG 1 cut(s) 1343
BspTNI GGTCTC 1 cut(s) 1403
BsrDI GCAATG 1 cut(s) 90
BsrI ACTGG 6 cut(s) 57, 454, 472, 1301, 1363, 1443
BssECI CCNNGG 2 cut(s) 838, 1322
BssMI GATC 4 cut(s) 122, 361, 1417, 1480
BssSI CACGAG 3 cut(s) 184, 939, 978
Bst2BI CACGAG 3 cut(s) 184, 939, 978
Bst2UI CCWGG 2 cut(s) 209, 1127
Bst4CI ACNGT 6 cut(s) 280, 442, 678, 824, 1087, 1150
Bst6I CTCTTC 2 cut(s) 145, 1056
BstAFI CTTAAG 1 cut(s) 1343
BstAPI GCANNNNNTGC 1 cut(s) 1199
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 3 cut(s) 715, 927, 1413
BstDEI CTNAG 8 cut(s) 729, 743, 867, 1044, 1104, 1281, 1387, 1434
BstDSI CCRYGG 1 cut(s) 838
BstEII GGTNACC 1 cut(s) 737
BstF5I GGATG 1 cut(s) 45
BstFNI CGCG 2 cut(s) 263, 717
BstHHI GCGC 1 cut(s) 263
BstKTI GATC 4 cut(s) 125, 364, 1420, 1483
BstMAI GTCTC 3 cut(s) 206, 1040, 1403
BstMBI GATC 4 cut(s) 122, 361, 1417, 1480
BstMCI CGRYCG 1 cut(s) 1519
BstMWI GCNNNNNNNGC 5 cut(s) 554, 714, 1199, 1208, 1370
BstNI CCWGG 2 cut(s) 209, 1127
BstNSI RCATGY 1 cut(s) 695
BstPAI GACNNNNGTC 1 cut(s) 744
BstPI GGTNACC 1 cut(s) 737
BstSCI CCNGG 4 cut(s) 195, 207, 1125, 1322
BstUI CGCG 2 cut(s) 263, 717
BstV1I GCAGC 3 cut(s) 880, 1223, 1252
BstV2I GAAGAC 1 cut(s) 1111
BsuRI GGCC 4 cut(s) 112, 170, 453, 973
BtgI CCRYGG 1 cut(s) 838
BtsCI GGATG 1 cut(s) 45
BtsI GCAGTG 1 cut(s) 429
BtsIMutI CAGTG 4 cut(s) 276, 429, 465, 674
BveI ACCTGC 3 cut(s) 286, 619, 867
Cac8I GCNNGC 3 cut(s) 715, 927, 1413
CaiI CAGNNNCTG 1 cut(s) 460
CfoI GCGC 1 cut(s) 263
Cfr13I GGNCC 3 cut(s) 110, 169, 284
CseI GACGC 1 cut(s) 112
Csp6I GTAC 4 cut(s) 246, 457, 721, 1285
CviAII CATG 4 cut(s) 489, 692, 913, 1484
CviQI GTAC 4 cut(s) 246, 457, 721, 1285
DdeI CTNAG 8 cut(s) 729, 743, 867, 1044, 1104, 1281, 1387, 1434
DpnI GATC 4 cut(s) 124, 363, 1419, 1482
DpnII GATC 4 cut(s) 122, 361, 1417, 1480
Eam1104I CTCTTC 2 cut(s) 145, 1056
EarI CTCTTC 2 cut(s) 145, 1056
Ecl136II GAGCTC 1 cut(s) 1573
Eco24I GRGCYC 1 cut(s) 1575
Eco31I GGTCTC 1 cut(s) 1403
Eco47I GGWCC 1 cut(s) 284
Eco53kI GAGCTC 1 cut(s) 1573
Eco57I CTGAAG 2 cut(s) 525, 1239
Eco91I GGTNACC 1 cut(s) 737
EcoICRI GAGCTC 1 cut(s) 1573
EcoO109I RGGNCCY 1 cut(s) 284
EcoO65I GGTNACC 1 cut(s) 737
EcoRI GAATTC 1 cut(s) 541
EcoRII CCWGG 2 cut(s) 207, 1125
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 1575
FaeI CATG 4 cut(s) 492, 695, 916, 1487
FalI AAGNNNNNCTT 4 cut(s) 28, 60, 1337, 1369
FaqI GGGAC 4 cut(s) 115, 270, 381, 660
FatI CATG 4 cut(s) 488, 691, 912, 1483
FauI CCCGC 1 cut(s) 722
FbaI TGATCA 2 cut(s) 361, 1480
Fnu4HI GCNGC 4 cut(s) 234, 894, 1212, 1266
FokI GGATG 1 cut(s) 52
FriOI GRGCYC 1 cut(s) 1575
Fsp4HI GCNGC 4 cut(s) 234, 894, 1212, 1266
GlaI GCGC 1 cut(s) 262
GluI GCNGC 4 cut(s) 234, 894, 1212, 1266
GsaI CCCAGC 1 cut(s) 110
GsuI CTGGAG 3 cut(s) 44, 74, 1380
HaeIII GGCC 4 cut(s) 112, 170, 453, 973
HapII CCGG 2 cut(s) 197, 1324
HgaI GACGC 1 cut(s) 112
HhaI GCGC 1 cut(s) 263
Hin1II CATG 4 cut(s) 492, 695, 916, 1487
Hin6I GCGC 1 cut(s) 261
HinP1I GCGC 1 cut(s) 261
HindIII AAGCTT 1 cut(s) 254
HinfI GANTC 7 cut(s) 10, 174, 805, 1048, 1184, 1436, 1457
HpaII CCGG 2 cut(s) 197, 1324
HphI GGTGA 4 cut(s) 197, 749, 1234, 1556
Hpy166II GTNNAC 5 cut(s) 246, 304, 465, 570, 1440
Hpy188I TCNGA 4 cut(s) 385, 505, 616, 750
Hpy188III TCNNGA 9 cut(s) 23, 120, 287, 1141, 1257, 1330, 1478, 1497, 1561
Hpy8I GTNNAC 5 cut(s) 246, 304, 465, 570, 1440
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 4 cut(s) 58, 865, 1108, 1219
HpyCH4III ACNGT 6 cut(s) 280, 442, 678, 824, 1087, 1150
HpyCH4IV ACGT 1 cut(s) 1158
HpyF10VI GCNNNNNNNGC 5 cut(s) 554, 714, 1199, 1208, 1370
HpyF3I CTNAG 8 cut(s) 729, 743, 867, 1044, 1104, 1281, 1387, 1434
HpySE526I ACGT 1 cut(s) 1158
Hsp92II CATG 4 cut(s) 492, 695, 916, 1487
HspAI GCGC 1 cut(s) 261
KflI GGGWCCC 1 cut(s) 284
Ksp22I TGATCA 2 cut(s) 361, 1480
Kzo9I GATC 4 cut(s) 122, 361, 1417, 1480
LmnI GCTCC 5 cut(s) 28, 55, 385, 1350, 1361
Lsp1109I GCAGC 3 cut(s) 880, 1223, 1252
LweI GCATC 5 cut(s) 1015, 1202, 1238, 1502, 1537
MaeII ACGT 1 cut(s) 1158
MaeIII GTNAC 4 cut(s) 424, 737, 908, 934
MalI GATC 4 cut(s) 124, 363, 1419, 1482
MboI GATC 4 cut(s) 122, 361, 1417, 1480
MboII GAAGA 8 cut(s) 103, 132, 372, 710, 1025, 1043, 1111, 1245
MhlI GDGCHC 1 cut(s) 1575
MluCI AATT 9 cut(s) 290, 400, 541, 660, 756, 768, 844, 1029, 1095
MlyI GAGTC 3 cut(s) 1042, 1445, 1466
MmeI TCCRAC 3 cut(s) 252, 474, 1141
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 702, 1118
MseI TTAA 4 cut(s) 240, 651, 788, 1344
MslI CAYNNNNRTG 1 cut(s) 1220
MspCI CTTAAG 1 cut(s) 1343
MspI CCGG 2 cut(s) 197, 1324
MspR9I CCNGG 4 cut(s) 197, 209, 1127, 1324
Mva1269I GAATGC 2 cut(s) 4, 157
MvaI CCWGG 2 cut(s) 209, 1127
MvnI CGCG 2 cut(s) 263, 717
MwoI GCNNNNNNNGC 5 cut(s) 554, 714, 1199, 1208, 1370
NciI CCSGG 2 cut(s) 197, 1324
NdeII GATC 4 cut(s) 122, 361, 1417, 1480
NlaIII CATG 4 cut(s) 492, 695, 916, 1487
NlaIV GGNNCC 6 cut(s) 30, 57, 111, 285, 286, 1363
NmuCI GTSAC 2 cut(s) 424, 737
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 695
NspV TTCGAA 1 cut(s) 8
PcsI WCGNNNNNNNCGW 1 cut(s) 110
PctI GAATGC 2 cut(s) 4, 157
PdmI GAANNNNTTC 2 cut(s) 702, 1118
PfeI GAWTC 4 cut(s) 10, 174, 805, 1184
PflMI CCANNNNNTGG 1 cut(s) 1540
PkrI GCNGC 4 cut(s) 235, 895, 1213, 1267
PleI GAGTC 3 cut(s) 1042, 1444, 1465
PpsI GAGTC 3 cut(s) 1042, 1444, 1465
PpuMI RGGWCCY 1 cut(s) 284
PshAI GACNNNNGTC 1 cut(s) 744
Psp124BI GAGCTC 1 cut(s) 1575
Psp5II RGGWCCY 1 cut(s) 284
Psp6I CCWGG 2 cut(s) 207, 1125
PspEI GGTNACC 1 cut(s) 737
PspFI CCCAGC 1 cut(s) 106
PspGI CCWGG 2 cut(s) 207, 1125
PspN4I GGNNCC 6 cut(s) 30, 57, 111, 285, 286, 1363
PspPI GGNCC 3 cut(s) 110, 169, 284
PspPPI RGGWCCY 1 cut(s) 284
PstNI CAGNNNCTG 1 cut(s) 460
RsaI GTAC 4 cut(s) 247, 458, 722, 1286
RsaNI GTAC 4 cut(s) 246, 457, 721, 1285
RseI CAYNNNNRTG 1 cut(s) 1220
SacI GAGCTC 1 cut(s) 1575
SaqAI TTAA 4 cut(s) 240, 651, 788, 1344
SatI GCNGC 4 cut(s) 234, 894, 1212, 1266
Sau3AI GATC 4 cut(s) 122, 361, 1417, 1480
Sau96I GGNCC 3 cut(s) 110, 169, 284
SchI GAGTC 3 cut(s) 1042, 1445, 1466
ScrFI CCNGG 4 cut(s) 197, 209, 1127, 1324
SduI GDGCHC 1 cut(s) 1575
SfaNI GCATC 5 cut(s) 1015, 1202, 1238, 1502, 1537
SfuI TTCGAA 1 cut(s) 8
SinI GGWCC 1 cut(s) 284
SmiMI CAYNNNNRTG 1 cut(s) 1220
SmlI CTYRAG 6 cut(s) 227, 329, 585, 967, 1328, 1343
SmoI CTYRAG 6 cut(s) 227, 329, 585, 967, 1328, 1343
Sse9I AATT 9 cut(s) 290, 400, 541, 660, 756, 768, 844, 1029, 1095
SsiI CCGC 4 cut(s) 233, 263, 715, 1202
SstI GAGCTC 1 cut(s) 1575
StyD4I CCNGG 4 cut(s) 195, 207, 1125, 1322
TaaI ACNGT 6 cut(s) 280, 442, 678, 824, 1087, 1150
TaiI ACGT 1 cut(s) 1161
TaqI TCGA 6 cut(s) 8, 47, 62, 177, 1423, 1575
TaqII GACCGA 2 cut(s) 974, 1533
TasI AATT 9 cut(s) 290, 400, 541, 660, 756, 768, 844, 1029, 1095
TauI GCSGC 1 cut(s) 236
TfiI GAWTC 4 cut(s) 10, 174, 805, 1184
Tru1I TTAA 4 cut(s) 240, 651, 788, 1344
Tru9I TTAA 4 cut(s) 240, 651, 788, 1344
TscAI CASTG 4 cut(s) 283, 429, 472, 681
TseFI GTSAC 2 cut(s) 424, 737
TseI GCWGC 3 cut(s) 893, 1211, 1265
Tsp45I GTSAC 2 cut(s) 424, 737
TspDTI ATGAA 2 cut(s) 436, 581
TspGWI ACGGA 1 cut(s) 1032
TspRI CASTG 4 cut(s) 283, 429, 472, 681
Van91I CCANNNNNTGG 1 cut(s) 1540
Vha464I CTTAAG 1 cut(s) 1343
VpaK11BI GGWCC 1 cut(s) 284
XapI RAATTY 4 cut(s) 541, 756, 844, 1095
XceI RCATGY 1 cut(s) 695
XmnI GAANNNNTTC 2 cut(s) 702, 1118
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.