Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3D

Orthogroup ID

OG0001999

Physical Location & Seq

Genomic Coordinates Forward (+)

15902476 .. 15908657

Gene Structure 6182 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3DG178700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1641 bp

ATGCATTCGAATCATCTGCTTCTGGAGGAGCCGATAAGGATGGCTTCGATATTGGAGCCGTCGAAGGCGAGTTTTTTCCCTGCAATGACGAAGATTGTGGGGACGCTGGGGCCGAAATCCCGATCTGTGGAGGTCATCTCTTCTTGCCTCAATGCTGGAATGTCTGTGGCCCGATTCGATTTCTCGTGGGGCAACCCGGAGTATCACCAGGAGACTTTGGACAATCTCAAGGCGGCTGTTAAGAGTACCAAGAAGCTTTGCGCGGTTATGTTGGACACTGTAGGTCCCGAATTGCAGGTTGTAAACAGAGGCGAGAAGATCATTGAACTCAAGGGAGATGGCTTGGTTATTCTTACCCCTGATCAGGGACAAGAACCATCTTCGGAGCTTTTGCCCATCAATTTTGATGGATTGTCAAAGGCAGTGACAAAGGGAGATACCGTTTTCATAGGCCAGTACCTGTTCACTGGAAGTGAAACTACTTCTGCATGGTTGGAGGTGTCTGAAGTGAAAGGAGATGATGTTGTTTGTGTGGTAAAGAATTCTGCAACATTGGCCGGTTCTTTGTTCACTTTGCATATCTCTCAAGTTCATATTGACTTGCCTACTCTCTCTGATAAAGATAAGCAGGTTATCAGTAGTTGGGGACTTAAAAACAAAATTGATTTCCTCTCACTGTCATATACACGACATGCAGAAGATGTTCGCCAAGCCCGCGAGTTCCTTTCTAAGTTGGGTGACCTCAGTCAGACACAAATTTTCGCTAAGATTGAAAATATGGAGGGATTAACCCATTTTGACGAGATTCTACAAGAAGCTGACGGTATTATCCTATCCCGTGGAAATTTGGGCATTGACCTGCCACCTGAGAAGGTGTTTTTATTTCAAAAGGCTGCCCTTTACAAGTGTAACATGGCTGGAAAGCCTGCTGTGGTTACTCGTGTTGTGGATAGTATGACTGACAATTTGAGGCCAACTCGTGCAGAAGCAACTGATGTTGCAAATGCTGTATTAGATGGAAGTGATGCAATTCTTCTCGGTGCTGAGACTCTTCGTGGGTTATACCCTGTTGATACCATCTCTACTGTTGGCAAAATTTGTGCTGAGGCAGAGAAGGTCTTTAACCAGGACTTGTATTTCAAGAGGACAGTCAAATATGTTGGAGAGCCAATGACACACTTGGAATCTATTGCATCCTCTGCGGTTCGGGCAGCCATTAAGGTGAAGGCATCTGTTATTATTTGCTTCACTTCTTCAGGACGGGCTGCAAGATTGATTGCTAAGTACCGACCCACAATGCCAGTTCTTTCAGTCGTCATCCCCCGGCTCAAGACAAATCAGCTTAAGTGGAGCTTTACTGGAGCCTTTGAGGAAGCTCATGAAAATTCTAGGTCTCTTGCAGATTTTAACGTTGACGTTAATGCTCAGGCAAGGCAATCACTCTTAGTCAGGGGTCTTTACCCTATGCTTGCTGATCCTCGACACCCTGCCGAGTCCACCAGTTCAACAAACGAATCAGTTCTAAAGGTTGCTCTTGATCATGGAAAGGCATCAGGAGTTGTAAAGTCGCACGACCGAGTAGTTGTTTGCCAGAAAGTTGGTGATGCCTCTGTTGTCAAGATTATTGAGCTTGAAGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230

Pfam Domains

Protein Families

PK PK_C

Protein Analysis

546

Amino Acids

59.53

Weight (kDa)

6.11

Isoelectric Point (pI)

30.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK	PF00224	30 - 360	1.3e-87	Pyruvate kinase, barrel domain
PK_C	PF02887	395 - 534	3.8e-23	Pyruvate kinase, alpha/beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001999)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36580 AT3G52990 AT3G52990
fragaria_vesca	FvH4_6g17480 FvH4_6g17480 FvH4_6g17480 FvH4_6g17480 FvH4_7g06490 FvH4_7g06490 FvH4_7g06490 FvH4_7g06490 FvH4_7g06490
malus_domestica	MD02G1244000.v1.1 MD07G1072900.v1.1 MD07G1073000.v1.1 MD12G1096900.v1.1 MD14G1094200.v1.1
prunus_persica	Prupe.2G091200_v2.0.a1 Prupe.2G091200_v2.0.a1 Prupe.2G091200_v2.0.a1 Prupe.7G120700_v2.0.a1 Prupe.7G120700_v2.0.a1
pyrus_communis	pycom07g05600 pycom07g05630 pycom12g09800 pycom14g09200
rosa_chinensis	RchiOBHm_Chr1g0334861 RchiOBHm_Chr3g0471721
rosa_laevigata	RLG00000024139 RLG00000029414
rosa_multiflora	Rmu_sc0000574.1_g000046 Rmu_sc0005631.1_g000026 Rmu_sc0026537.1_g000003
rosa_roxburghii	Rroxscaffold_4G00316390 Rroxscaffold_6G00409610
rosa_rugosa	Rorug01G0117400 Rorug03G0119000 Rorug03G0119100 Rorug03G0119200
rosa_samantha	Rh1CG133000 Rh3AG170600 Rh3BG196300 Rh3DG178700
rosa_wichuraiana	Rw0G009040 Rw1G011670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	286, 619, 867
AccII	CGCG	2 cut(s)	263, 717
AciI	CCGC	4 cut(s)	233, 263, 715, 1202
AclI	AACGTT	1 cut(s)	1410
AclWI	GGATC	1 cut(s)	1469
AcoI	YGGCCR	1 cut(s)	555
AcsI	RAATTY	5 cut(s)	541, 756, 844, 1095, 1384
AcuI	CTGAAG	2 cut(s)	525, 1239
AfaI	GTAC	3 cut(s)	247, 458, 1286
AfiI	CCNNNNNNNGG	3 cut(s)	127, 364, 365
AflII	CTTAAG	1 cut(s)	1343
AgsI	TTSAA	6 cut(s)	326, 773, 887, 1141, 1506, 1634
AjnI	CCWGG	2 cut(s)	207, 1125
AluBI	AGCT	7 cut(s)	256, 388, 818, 1342, 1353, 1376, 1630
AluI	AGCT	7 cut(s)	256, 388, 818, 1342, 1353, 1376, 1630
Alw26I	GTCTC	3 cut(s)	206, 1040, 1398
AlwI	GGATC	1 cut(s)	1469
AlwNI	CAGNNNCTG	1 cut(s)	460
AoxI	GGCC	5 cut(s)	110, 168, 451, 555, 971
ApeKI	GCWGC	3 cut(s)	893, 1211, 1265
ApoI	RAATTY	5 cut(s)	541, 756, 844, 1095, 1384
ArsI	GACNNNNNNTTYG	2 cut(s)	949, 981
Asp700I	GAANNNNTTC	2 cut(s)	702, 1518
AspLEI	GCGC	1 cut(s)	263
AspS9I	GGNCC	3 cut(s)	110, 169, 284
AsuC2I	CCSGG	2 cut(s)	197, 1324
AsuHPI	GGTGA	4 cut(s)	197, 749, 1234, 1613
AsuII	TTCGAA	1 cut(s)	8
AvaII	GGWCC	1 cut(s)	284
BarI	GAAGNNNNNNTAC	2 cut(s)	463, 495
BauI	CACGAG	3 cut(s)	184, 939, 978
BbvCI	CCTCAGC	1 cut(s)	1104
BbvI	GCAGC	3 cut(s)	880, 1223, 1252
BccI	CCATC	7 cut(s)	34, 332, 385, 401, 404, 1010, 1085
BceAI	ACGGC	1 cut(s)	43
BcgI	CGANNNNNNTGC	1 cut(s)	32
BciT130I	CCWGG	2 cut(s)	209, 1127
BclI	TGATCA	2 cut(s)	361, 1537
BcnI	CCSGG	2 cut(s)	197, 1324
BcoDI	GTCTC	3 cut(s)	206, 1040, 1398
BfaI	CTAG	1 cut(s)	1389
BfmI	CTRYAG	1 cut(s)	279
BfrI	CTTAAG	1 cut(s)	1343
BfuAI	ACCTGC	3 cut(s)	286, 619, 867
BisI	GCNGC	4 cut(s)	234, 894, 1212, 1266
BlsI	GCNGC	4 cut(s)	235, 895, 1213, 1267
Bme1390I	CCNGG	4 cut(s)	197, 209, 1127, 1324
Bme18I	GGWCC	1 cut(s)	284
BmgT120I	GGNCC	3 cut(s)	110, 169, 284
BmiI	GGNNCC	5 cut(s)	30, 57, 111, 286, 1363
BmrFI	CCNGG	4 cut(s)	197, 209, 1127, 1324
BmsI	GCATC	5 cut(s)	1015, 1202, 1238, 1559, 1594
BoxI	GACNNNNGTC	1 cut(s)	744
BplI	GAGNNNNNCTC	4 cut(s)	122, 154, 961, 993
BpmI	CTGGAG	2 cut(s)	44, 1380
Bpu10I	CCTNAGC	2 cut(s)	1104, 1425
Bpu14I	TTCGAA	1 cut(s)	8
BpuEI	CTTGAG	4 cut(s)	212, 314, 570, 1313
BpuMI	CCSGG	2 cut(s)	197, 1324
BsaI	GGTCTC	1 cut(s)	1398
BsaJI	CCNNGG	2 cut(s)	838, 1322
BsaXI	ACNNNNNCTCC	4 cut(s)	426, 456, 507, 537
Bsc4I	CCNNNNNNNGG	3 cut(s)	127, 364, 365
Bse118I	RCCGGY	1 cut(s)	557
Bse1I	ACTGG	5 cut(s)	454, 472, 1301, 1363, 1500
Bse3DI	GCAATG	1 cut(s)	90
BseBI	CCWGG	2 cut(s)	209, 1127
BseDI	CCNNGG	2 cut(s)	838, 1322
BseGI	GGATG	3 cut(s)	45, 1193, 1317
BseLI	CCNNNNNNNGG	3 cut(s)	127, 364, 365
BseMI	GCAATG	1 cut(s)	90
BseMII	CTCAG	5 cut(s)	757, 858, 1035, 1095, 1439
BseNI	ACTGG	5 cut(s)	454, 472, 1301, 1363, 1500
BseRI	GAGGAG	1 cut(s)	41
BseXI	GCAGC	3 cut(s)	880, 1223, 1252
BseYI	CCCAGC	1 cut(s)	106
BsgI	GTGCAG	1 cut(s)	1002
Bsh1236I	CGCG	2 cut(s)	263, 717
Bsh1285I	CGRYCG	1 cut(s)	1576
BshFI	GGCC	5 cut(s)	112, 170, 453, 557, 973
BsiEI	CGRYCG	1 cut(s)	1576
BsiSI	CCGG	3 cut(s)	197, 558, 1324
BslFI	GGGAC	4 cut(s)	115, 270, 381, 660
BslI	CCNNNNNNNGG	3 cut(s)	127, 364, 365
BsmAI	GTCTC	3 cut(s)	206, 1040, 1398
BsmFI	GGGAC	4 cut(s)	115, 270, 381, 660
BsmI	GAATGC	1 cut(s)	4
BsnI	GGCC	5 cut(s)	112, 170, 453, 557, 973
Bso31I	GGTCTC	1 cut(s)	1398
Bsp119I	TTCGAA	1 cut(s)	8
Bsp143I	GATC	5 cut(s)	122, 318, 361, 1474, 1537
BspACI	CCGC	4 cut(s)	233, 263, 715, 1202
BspANI	GGCC	5 cut(s)	112, 170, 453, 557, 973
BspCNI	CTCAG	5 cut(s)	756, 859, 1036, 1096, 1438
BspFNI	CGCG	2 cut(s)	263, 717
BspHI	TCATGA	1 cut(s)	1378
BspLI	GGNNCC	5 cut(s)	30, 57, 111, 286, 1363
BspMI	ACCTGC	3 cut(s)	286, 619, 867
BspPI	GGATC	1 cut(s)	1469
BspT104I	TTCGAA	1 cut(s)	8
BspTI	CTTAAG	1 cut(s)	1343
BspTNI	GGTCTC	1 cut(s)	1398
BsrDI	GCAATG	1 cut(s)	90
BsrFI	RCCGGY	1 cut(s)	557
BsrI	ACTGG	5 cut(s)	454, 472, 1301, 1363, 1500
BssAI	RCCGGY	1 cut(s)	557
BssECI	CCNNGG	2 cut(s)	838, 1322
BssMI	GATC	5 cut(s)	122, 318, 361, 1474, 1537
BssSI	CACGAG	3 cut(s)	184, 939, 978
Bst2BI	CACGAG	3 cut(s)	184, 939, 978
Bst2UI	CCWGG	2 cut(s)	209, 1127
Bst4CI	ACNGT	6 cut(s)	280, 442, 678, 824, 1087, 1150
Bst6I	CTCTTC	2 cut(s)	145, 1056
BstAFI	CTTAAG	1 cut(s)	1343
BstAPI	GCANNNNNTGC	1 cut(s)	1199
BstBI	TTCGAA	1 cut(s)	8
BstC8I	GCNNGC	3 cut(s)	715, 927, 1470
BstDEI	CTNAG	9 cut(s)	729, 743, 765, 867, 1044, 1104, 1281, 1425, 1444
BstDSI	CCRYGG	1 cut(s)	838
BstEII	GGTNACC	1 cut(s)	737
BstF5I	GGATG	3 cut(s)	45, 1193, 1317
BstFNI	CGCG	2 cut(s)	263, 717
BstHHI	GCGC	1 cut(s)	263
BstKTI	GATC	5 cut(s)	125, 321, 364, 1477, 1540
BstMAI	GTCTC	3 cut(s)	206, 1040, 1398
BstMBI	GATC	5 cut(s)	122, 318, 361, 1474, 1537
BstMCI	CGRYCG	1 cut(s)	1576
BstMWI	GCNNNNNNNGC	4 cut(s)	554, 714, 1199, 1208
BstNI	CCWGG	2 cut(s)	209, 1127
BstNSI	RCATGY	1 cut(s)	695
BstPAI	GACNNNNGTC	1 cut(s)	744
BstPI	GGTNACC	1 cut(s)	737
BstSCI	CCNGG	4 cut(s)	195, 207, 1125, 1322
BstSFI	CTRYAG	1 cut(s)	279
BstUI	CGCG	2 cut(s)	263, 717
BstV1I	GCAGC	3 cut(s)	880, 1223, 1252
BstXI	CCANNNNNNTGG	1 cut(s)	1598
BsuRI	GGCC	5 cut(s)	112, 170, 453, 557, 973
BtgI	CCRYGG	1 cut(s)	838
BtsCI	GGATG	3 cut(s)	45, 1193, 1317
BtsI	GCAGTG	1 cut(s)	429
BtsIMutI	CAGTG	4 cut(s)	276, 429, 465, 674
BveI	ACCTGC	3 cut(s)	286, 619, 867
Cac8I	GCNNGC	3 cut(s)	715, 927, 1470
CaiI	CAGNNNCTG	1 cut(s)	460
CciI	TCATGA	1 cut(s)	1378
CfoI	GCGC	1 cut(s)	263
Cfr10I	RCCGGY	1 cut(s)	557
Cfr13I	GGNCC	3 cut(s)	110, 169, 284
CseI	GACGC	1 cut(s)	112
Csp6I	GTAC	3 cut(s)	246, 457, 1285
CviAII	CATG	5 cut(s)	489, 692, 913, 1379, 1541
CviQI	GTAC	3 cut(s)	246, 457, 1285
DdeI	CTNAG	9 cut(s)	729, 743, 765, 867, 1044, 1104, 1281, 1425, 1444
DpnI	GATC	5 cut(s)	124, 320, 363, 1476, 1539
DpnII	GATC	5 cut(s)	122, 318, 361, 1474, 1537
EaeI	YGGCCR	1 cut(s)	555
Eam1104I	CTCTTC	2 cut(s)	145, 1056
EarI	CTCTTC	2 cut(s)	145, 1056
Eco31I	GGTCTC	1 cut(s)	1398
Eco47I	GGWCC	1 cut(s)	284
Eco57I	CTGAAG	2 cut(s)	525, 1239
Eco91I	GGTNACC	1 cut(s)	737
EcoO109I	RGGNCCY	1 cut(s)	284
EcoO65I	GGTNACC	1 cut(s)	737
EcoRI	GAATTC	1 cut(s)	541
EcoRII	CCWGG	2 cut(s)	207, 1125
EcoT22I	ATGCAT	1 cut(s)	6
FaeI	CATG	5 cut(s)	492, 695, 916, 1382, 1544
FalI	AAGNNNNNCTT	4 cut(s)	28, 60, 1337, 1369
FaqI	GGGAC	4 cut(s)	115, 270, 381, 660
FatI	CATG	5 cut(s)	488, 691, 912, 1378, 1540
FauI	CCCGC	1 cut(s)	722
FbaI	TGATCA	2 cut(s)	361, 1537
Fnu4HI	GCNGC	4 cut(s)	234, 894, 1212, 1266
FokI	GGATG	3 cut(s)	52, 1180, 1304
Fsp4HI	GCNGC	4 cut(s)	234, 894, 1212, 1266
FspBI	CTAG	1 cut(s)	1389
GlaI	GCGC	1 cut(s)	262
GluI	GCNGC	4 cut(s)	234, 894, 1212, 1266
GsaI	CCCAGC	1 cut(s)	110
GsuI	CTGGAG	2 cut(s)	44, 1380
HaeIII	GGCC	5 cut(s)	112, 170, 453, 557, 973
HapII	CCGG	3 cut(s)	197, 558, 1324
HgaI	GACGC	1 cut(s)	112
HhaI	GCGC	1 cut(s)	263
Hin1II	CATG	5 cut(s)	492, 695, 916, 1382, 1544
Hin6I	GCGC	1 cut(s)	261
HinP1I	GCGC	1 cut(s)	261
HincII	GTYRAC	1 cut(s)	1414
HindII	GTYRAC	1 cut(s)	1414
HindIII	AAGCTT	1 cut(s)	254
HinfI	GANTC	7 cut(s)	10, 174, 805, 1048, 1184, 1493, 1514
HpaII	CCGG	3 cut(s)	197, 558, 1324
HphI	GGTGA	4 cut(s)	197, 749, 1234, 1613
Hpy166II	GTNNAC	5 cut(s)	304, 465, 570, 1414, 1497
Hpy188I	TCNGA	4 cut(s)	385, 505, 616, 750
Hpy8I	GTNNAC	5 cut(s)	304, 465, 570, 1414, 1497
Hpy99I	CGWCG	1 cut(s)	64
HpyAV	CCTTC	4 cut(s)	58, 865, 1108, 1219
HpyCH4III	ACNGT	6 cut(s)	280, 442, 678, 824, 1087, 1150
HpyCH4IV	ACGT	2 cut(s)	1410, 1416
HpyF10VI	GCNNNNNNNGC	4 cut(s)	554, 714, 1199, 1208
HpyF3I	CTNAG	9 cut(s)	729, 743, 765, 867, 1044, 1104, 1281, 1425, 1444
HpySE526I	ACGT	2 cut(s)	1410, 1416
Hsp92II	CATG	5 cut(s)	492, 695, 916, 1382, 1544
HspAI	GCGC	1 cut(s)	261
Ksp22I	TGATCA	2 cut(s)	361, 1537
Kzo9I	GATC	5 cut(s)	122, 318, 361, 1474, 1537
LmnI	GCTCC	5 cut(s)	28, 55, 385, 1350, 1361
Lsp1109I	GCAGC	3 cut(s)	880, 1223, 1252
LweI	GCATC	5 cut(s)	1015, 1202, 1238, 1559, 1594
MaeI	CTAG	1 cut(s)	1389
MaeII	ACGT	2 cut(s)	1410, 1416
MaeIII	GTNAC	4 cut(s)	424, 737, 908, 934
MalI	GATC	5 cut(s)	124, 320, 363, 1476, 1539
MboI	GATC	5 cut(s)	122, 318, 361, 1474, 1537
MboII	GAAGA	8 cut(s)	103, 132, 328, 372, 710, 1025, 1043, 1245
MlyI	GAGTC	2 cut(s)	1042, 1502
MmeI	TCCRAC	3 cut(s)	252, 474, 1141
Mph1103I	ATGCAT	1 cut(s)	6
MroXI	GAANNNNTTC	2 cut(s)	702, 1518
MseI	TTAA	8 cut(s)	240, 651, 788, 1122, 1218, 1344, 1407, 1419
MslI	CAYNNNNRTG	1 cut(s)	1220
MspCI	CTTAAG	1 cut(s)	1343
MspI	CCGG	3 cut(s)	197, 558, 1324
MspR9I	CCNGG	4 cut(s)	197, 209, 1127, 1324
Mva1269I	GAATGC	1 cut(s)	4
MvaI	CCWGG	2 cut(s)	209, 1127
MvnI	CGCG	2 cut(s)	263, 717
MwoI	GCNNNNNNNGC	4 cut(s)	554, 714, 1199, 1208
NciI	CCSGG	2 cut(s)	197, 1324
NdeII	GATC	5 cut(s)	122, 318, 361, 1474, 1537
NlaIII	CATG	5 cut(s)	492, 695, 916, 1382, 1544
NlaIV	GGNNCC	5 cut(s)	30, 57, 111, 286, 1363
NmeAIII	GCCGAG	1 cut(s)	1516
NmuCI	GTSAC	2 cut(s)	424, 737
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	1 cut(s)	695
NspV	TTCGAA	1 cut(s)	8
PagI	TCATGA	1 cut(s)	1378
PcsI	WCGNNNNNNNCGW	1 cut(s)	110
PctI	GAATGC	1 cut(s)	4
PdmI	GAANNNNTTC	2 cut(s)	702, 1518
PfeI	GAWTC	5 cut(s)	10, 174, 805, 1184, 1514
PkrI	GCNGC	4 cut(s)	235, 895, 1213, 1267
PleI	GAGTC	2 cut(s)	1042, 1501
PpsI	GAGTC	2 cut(s)	1042, 1501
PpuMI	RGGWCCY	1 cut(s)	284
PshAI	GACNNNNGTC	1 cut(s)	744
Psp1406I	AACGTT	1 cut(s)	1410
Psp5II	RGGWCCY	1 cut(s)	284
Psp6I	CCWGG	2 cut(s)	207, 1125
PspEI	GGTNACC	1 cut(s)	737
PspFI	CCCAGC	1 cut(s)	106
PspGI	CCWGG	2 cut(s)	207, 1125
PspN4I	GGNNCC	5 cut(s)	30, 57, 111, 286, 1363
PspPI	GGNCC	3 cut(s)	110, 169, 284
PspPPI	RGGWCCY	1 cut(s)	284
PstNI	CAGNNNCTG	1 cut(s)	460
RsaI	GTAC	3 cut(s)	247, 458, 1286
RsaNI	GTAC	3 cut(s)	246, 457, 1285
RseI	CAYNNNNRTG	1 cut(s)	1220
SaqAI	TTAA	8 cut(s)	240, 651, 788, 1122, 1218, 1344, 1407, 1419
SatI	GCNGC	4 cut(s)	234, 894, 1212, 1266
Sau3AI	GATC	5 cut(s)	122, 318, 361, 1474, 1537
Sau96I	GGNCC	3 cut(s)	110, 169, 284
SchI	GAGTC	2 cut(s)	1042, 1502
ScrFI	CCNGG	4 cut(s)	197, 209, 1127, 1324
SfaNI	GCATC	5 cut(s)	1015, 1202, 1238, 1559, 1594
SfcI	CTRYAG	1 cut(s)	279
SfuI	TTCGAA	1 cut(s)	8
SinI	GGWCC	1 cut(s)	284
SmiMI	CAYNNNNRTG	1 cut(s)	1220
SmlI	CTYRAG	5 cut(s)	227, 329, 585, 1328, 1343
SmoI	CTYRAG	5 cut(s)	227, 329, 585, 1328, 1343
SsiI	CCGC	4 cut(s)	233, 263, 715, 1202
SspMI	CTAG	1 cut(s)	1389
StyD4I	CCNGG	4 cut(s)	195, 207, 1125, 1322
TaaI	ACNGT	6 cut(s)	280, 442, 678, 824, 1087, 1150
TaiI	ACGT	2 cut(s)	1413, 1419
TaqI	TCGA	5 cut(s)	8, 47, 62, 177, 1480
TaqII	GACCGA	1 cut(s)	1590
TauI	GCSGC	1 cut(s)	236
TfiI	GAWTC	5 cut(s)	10, 174, 805, 1184, 1514
Tru1I	TTAA	8 cut(s)	240, 651, 788, 1122, 1218, 1344, 1407, 1419
Tru9I	TTAA	8 cut(s)	240, 651, 788, 1122, 1218, 1344, 1407, 1419
TscAI	CASTG	4 cut(s)	283, 429, 472, 681
TseFI	GTSAC	2 cut(s)	424, 737
TseI	GCWGC	3 cut(s)	893, 1211, 1265
Tsp45I	GTSAC	2 cut(s)	424, 737
TspDTI	ATGAA	3 cut(s)	436, 581, 1395
TspRI	CASTG	4 cut(s)	283, 429, 472, 681
Vha464I	CTTAAG	1 cut(s)	1343
VpaK11BI	GGWCC	1 cut(s)	284
XapI	RAATTY	5 cut(s)	541, 756, 844, 1095, 1384
XceI	RCATGY	1 cut(s)	695
XmnI	GAANNNNTTC	2 cut(s)	702, 1518
XspI	CTAG	1 cut(s)	1389
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.