Rorug03G0119000

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001999
Physical Location & Seq
Forward (+)
9641932 .. 9642749
818 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0119000.1

Sequence Viewer

Length: 789 bp
ATGGTACTGCATTCTTCTCTATTTCAAGCAATAACCTCAACGGGATCATTCCAGAATGCAATATGCAATTCACCTTATCTTGAGGTTCTTGATATGTCCAATAATTCTTTCACTGGCACGGTTCCTCAGTGCGTCACTACAATGAGAAAACTTAATGTGCTTAATCTAAGGAGAAACAAGTTTACACATGTTAATTTACCTCAGAATTGCAATTTAAGAAGTCTAGATGTGAGTGCAAATCAGTTAAAAGGTCAGTTTCCAAGATCTCTTGTCAATTGCACAGAGTTGGAGGTTTTAAACCTCGGAAGCAATCAGATAACAGATACATTTCCCTGCTTTTTGAAGAATATATCCACCTTGTGTATCCTTGTTTTACGGTCCAACAACTTTTATGGATCCACTGGTTGTCCAGAGACTAATGGCACCTGGCCAATGCTCCAGATCATAGATCTAGCTTATAACAATTTTGATGGTGAAATACCTGGAAGATCTTTATCAACCTGGAAGGCAATGATGGCAAATGAAGCCGGGGCCGCATCAAATCATCTTAAAATTCAGGGAGATGCAGCTGCAGTTAGTAGCAAAGGTTTACAGATGAACCTGGTAAAGATTTTAACTGTGTTCACCTTGATTGACTTCTCATGCAACAAGTTCAATGGATCAATGCTCGAGGAAATTGGAGAATTCAAATCACTACTAGTCCTCAACTTGTCGAATAATGCTTTCACAGGTGCAATTCCATCATCATTAAGTAATTTGCAAGAAGTCGAGTCTTTAGACCTCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

262

Amino Acids

28.68

Weight (kDa)

5.92

Isoelectric Point (pI)

37.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 71 - 130 6.5e-06 Leucine rich repeat
LRR_4 PF12799 71 - 109 2.5e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 459
AccB1I GGYRCC 1 cut(s) 422
AciI CCGC 1 cut(s) 534
AclWI GGATC 4 cut(s) 52, 390, 403, 667
AcoI YGGCCR 1 cut(s) 428
AcsI RAATTY 2 cut(s) 552, 683
AdeI CACNNNGTG 1 cut(s) 360
AfaI GTAC 1 cut(s) 6
AflIII ACRYGT 1 cut(s) 187
AgsI TTSAA 4 cut(s) 26, 343, 655, 688
AhlI ACTAGT 1 cut(s) 697
AjnI CCWGG 4 cut(s) 425, 481, 500, 600
AjuI GAANNNNNNNTTGG 2 cut(s) 92, 124
AluBI AGCT 2 cut(s) 455, 569
AluI AGCT 2 cut(s) 455, 569
Alw26I GTCTC 1 cut(s) 407
AlwI GGATC 4 cut(s) 52, 390, 403, 667
Ama87I CYCGRG 1 cut(s) 668
AoxI GGCC 2 cut(s) 428, 531
ApeKI GCWGC 2 cut(s) 566, 569
ApoI RAATTY 2 cut(s) 552, 683
AspS9I GGNCC 2 cut(s) 378, 531
AsuC2I CCSGG 1 cut(s) 529
AsuHPI GGTGA 3 cut(s) 63, 485, 616
AvaI CYCGRG 1 cut(s) 668
AvaII GGWCC 1 cut(s) 378
BalI TGGCCA 1 cut(s) 430
BamHI GGATCC 1 cut(s) 395
BanI GGYRCC 1 cut(s) 422
BbvI GCAGC 2 cut(s) 556, 578
BccI CCATC 3 cut(s) 464, 508, 748
BciT130I CCWGG 4 cut(s) 427, 483, 502, 602
BciVI GTATCC 1 cut(s) 374
BcnI CCSGG 1 cut(s) 529
BcoDI GTCTC 1 cut(s) 407
BcuI ACTAGT 1 cut(s) 697
BfaI CTAG 3 cut(s) 224, 452, 698
BfmI CTRYAG 1 cut(s) 570
BfuI GTATCC 1 cut(s) 374
BglII AGATCT 3 cut(s) 263, 448, 488
BisI GCNGC 3 cut(s) 534, 567, 570
BlsI GCNGC 3 cut(s) 535, 568, 571
Bme1390I CCNGG 5 cut(s) 427, 483, 502, 529, 602
Bme18I GGWCC 1 cut(s) 378
BmeT110I CYCGRG 1 cut(s) 668
BmgT120I GGNCC 2 cut(s) 378, 531
BmiI GGNNCC 4 cut(s) 123, 397, 424, 532
BmrFI CCNGG 5 cut(s) 427, 483, 502, 529, 602
BmsI GCATC 2 cut(s) 545, 553
BpmI CTGGAG 1 cut(s) 422
BpuEI CTTGAG 1 cut(s) 101
BpuMI CCSGG 1 cut(s) 529
BsaBI GATNNNNATC 1 cut(s) 493
BsaJI CCNNGG 2 cut(s) 301, 528
Bse1I ACTGG 2 cut(s) 118, 406
Bse3DI GCAATG 1 cut(s) 516
Bse8I GATNNNNATC 1 cut(s) 493
BseBI CCWGG 4 cut(s) 427, 483, 502, 602
BseDI CCNNGG 2 cut(s) 301, 528
BseJI GATNNNNATC 1 cut(s) 493
BseMI GCAATG 1 cut(s) 516
BseMII CTCAG 2 cut(s) 140, 215
BseNI ACTGG 2 cut(s) 118, 406
BseXI GCAGC 2 cut(s) 556, 578
BshFI GGCC 2 cut(s) 430, 533
BshNI GGYRCC 1 cut(s) 422
BsiHKCI CYCGRG 1 cut(s) 668
BsiSI CCGG 1 cut(s) 528
BsmAI GTCTC 1 cut(s) 407
BsmI GAATGC 2 cut(s) 10, 61
BsnI GGCC 2 cut(s) 430, 533
BsoBI CYCGRG 1 cut(s) 668
Bsp143I GATC 7 cut(s) 44, 263, 395, 441, 448, 488, 659
BspACI CCGC 1 cut(s) 534
BspANI GGCC 2 cut(s) 430, 533
BspCNI CTCAG 2 cut(s) 139, 214
BspLI GGNNCC 4 cut(s) 123, 397, 424, 532
BspMAI CTGCAG 1 cut(s) 574
BspPI GGATC 4 cut(s) 52, 390, 403, 667
BspT107I GGYRCC 1 cut(s) 422
BsrDI GCAATG 1 cut(s) 516
BsrI ACTGG 2 cut(s) 118, 406
BssECI CCNNGG 2 cut(s) 301, 528
BssMI GATC 7 cut(s) 44, 263, 395, 441, 448, 488, 659
Bst2UI CCWGG 4 cut(s) 427, 483, 502, 602
Bst4CI ACNGT 3 cut(s) 121, 378, 619
BstDEI CTNAG 3 cut(s) 126, 167, 201
BstKTI GATC 7 cut(s) 47, 266, 398, 444, 451, 491, 662
BstMAI GTCTC 1 cut(s) 407
BstMBI GATC 7 cut(s) 44, 263, 395, 441, 448, 488, 659
BstMWI GCNNNNNNNGC 3 cut(s) 515, 524, 533
BstNI CCWGG 4 cut(s) 427, 483, 502, 602
BstNSI RCATGY 1 cut(s) 191
BstSCI CCNGG 5 cut(s) 425, 481, 500, 527, 600
BstSFI CTRYAG 1 cut(s) 570
BstV1I GCAGC 2 cut(s) 556, 578
BstX2I RGATCY 4 cut(s) 263, 395, 448, 488
BstYI RGATCY 4 cut(s) 263, 395, 448, 488
BsuI GTATCC 1 cut(s) 374
BsuRI GGCC 2 cut(s) 430, 533
BtsIMutI CAGTG 3 cut(s) 111, 134, 399
Cfr13I GGNCC 2 cut(s) 378, 531
CseI GACGC 1 cut(s) 121
CsiI ACCWGGT 1 cut(s) 600
Csp6I GTAC 1 cut(s) 5
CviAII CATG 2 cut(s) 188, 642
CviJI RGCY 5 cut(s) 430, 455, 527, 533, 569
CviKI_1 RGCY 5 cut(s) 430, 455, 527, 533, 569
CviQI GTAC 1 cut(s) 5
DdeI CTNAG 3 cut(s) 126, 167, 201
DpnI GATC 7 cut(s) 46, 265, 397, 443, 450, 490, 661
DpnII GATC 7 cut(s) 44, 263, 395, 441, 448, 488, 659
DraI TTTAAA 1 cut(s) 297
DraIII CACNNNGTG 1 cut(s) 360
EaeI YGGCCR 1 cut(s) 428
Eco47I GGWCC 1 cut(s) 378
Eco88I CYCGRG 1 cut(s) 668
EcoRI GAATTC 1 cut(s) 683
EcoRII CCWGG 4 cut(s) 425, 481, 500, 600
FaeI CATG 2 cut(s) 191, 645
FaiI YATR 9 cut(s) 64, 95, 189, 350, 393, 446, 459, 643, 787
FatI CATG 2 cut(s) 187, 641
Fnu4HI GCNGC 3 cut(s) 534, 567, 570
Fsp4HI GCNGC 3 cut(s) 534, 567, 570
FspBI CTAG 3 cut(s) 224, 452, 698
GluI GCNGC 3 cut(s) 534, 567, 570
GsuI CTGGAG 1 cut(s) 422
HaeIII GGCC 2 cut(s) 430, 533
HapII CCGG 1 cut(s) 528
HgaI GACGC 1 cut(s) 121
Hin1II CATG 2 cut(s) 191, 645
HinfI GANTC 1 cut(s) 770
HpaII CCGG 1 cut(s) 528
HphI GGTGA 3 cut(s) 63, 485, 616
Hpy166II GTNNAC 3 cut(s) 183, 590, 624
Hpy188I TCNGA 3 cut(s) 204, 305, 315
Hpy188III TCNNGA 6 cut(s) 52, 80, 89, 224, 410, 439
Hpy8I GTNNAC 3 cut(s) 183, 590, 624
HpyAV CCTTC 1 cut(s) 499
HpyCH4III ACNGT 3 cut(s) 121, 378, 619
HpyF10VI GCNNNNNNNGC 3 cut(s) 515, 524, 533
HpyF3I CTNAG 3 cut(s) 126, 167, 201
Hsp92II CATG 2 cut(s) 191, 645
Kzo9I GATC 7 cut(s) 44, 263, 395, 441, 448, 488, 659
LmnI GCTCC 1 cut(s) 441
Lsp1109I GCAGC 2 cut(s) 556, 578
LweI GCATC 2 cut(s) 545, 553
MabI ACCWGGT 1 cut(s) 600
MaeI CTAG 3 cut(s) 224, 452, 698
MaeIII GTNAC 1 cut(s) 133
MalI GATC 7 cut(s) 46, 265, 397, 443, 450, 490, 661
MboI GATC 7 cut(s) 44, 263, 395, 441, 448, 488, 659
MboII GAAGA 3 cut(s) 6, 355, 498
MfeI CAATTG 1 cut(s) 274
MflI RGATCY 4 cut(s) 263, 395, 448, 488
MlsI TGGCCA 1 cut(s) 430
MluNI TGGCCA 1 cut(s) 430
MlyI GAGTC 1 cut(s) 779
MmeI TCCRAC 2 cut(s) 267, 405
MnlI CCTC 8 cut(s) 46, 76, 135, 210, 283, 311, 664, 713
Mox20I TGGCCA 1 cut(s) 430
MscI TGGCCA 1 cut(s) 430
MseI TTAA 9 cut(s) 153, 162, 192, 215, 245, 296, 549, 614, 749
MslI CAYNNNNRTG 1 cut(s) 140
Msp20I TGGCCA 1 cut(s) 430
MspA1I CMGCKG 1 cut(s) 569
MspI CCGG 1 cut(s) 528
MspR9I CCNGG 5 cut(s) 427, 483, 502, 529, 602
MunI CAATTG 1 cut(s) 274
Mva1269I GAATGC 2 cut(s) 10, 61
MvaI CCWGG 4 cut(s) 427, 483, 502, 602
MwoI GCNNNNNNNGC 3 cut(s) 515, 524, 533
NciI CCSGG 1 cut(s) 529
NdeII GATC 7 cut(s) 44, 263, 395, 441, 448, 488, 659
NlaIII CATG 2 cut(s) 191, 645
NlaIV GGNNCC 4 cut(s) 123, 397, 424, 532
NmuCI GTSAC 1 cut(s) 133
NspI RCATGY 1 cut(s) 191
PaeR7I CTCGAG 1 cut(s) 668
PciI ACATGT 1 cut(s) 187
PctI GAATGC 2 cut(s) 10, 61
PkrI GCNGC 3 cut(s) 535, 568, 571
PleI GAGTC 1 cut(s) 778
PpsI GAGTC 1 cut(s) 778
PscI ACATGT 1 cut(s) 187
PsiI TTATAA 1 cut(s) 459
Psp6I CCWGG 4 cut(s) 425, 481, 500, 600
PspGI CCWGG 4 cut(s) 425, 481, 500, 600
PspN4I GGNNCC 4 cut(s) 123, 397, 424, 532
PspPI GGNCC 2 cut(s) 378, 531
PspXI VCTCGAGB 1 cut(s) 668
PstI CTGCAG 1 cut(s) 574
PsuI RGATCY 4 cut(s) 263, 395, 448, 488
PvuII CAGCTG 1 cut(s) 569
RsaI GTAC 1 cut(s) 6
RsaNI GTAC 1 cut(s) 5
RseI CAYNNNNRTG 1 cut(s) 140
SaqAI TTAA 9 cut(s) 153, 162, 192, 215, 245, 296, 549, 614, 749
SatI GCNGC 3 cut(s) 534, 567, 570
Sau3AI GATC 7 cut(s) 44, 263, 395, 441, 448, 488, 659
Sau96I GGNCC 2 cut(s) 378, 531
SchI GAGTC 1 cut(s) 779
ScrFI CCNGG 5 cut(s) 427, 483, 502, 529, 602
SexAI ACCWGGT 1 cut(s) 600
SfaNI GCATC 2 cut(s) 545, 553
SfcI CTRYAG 1 cut(s) 570
Sfr274I CTCGAG 1 cut(s) 668
SinI GGWCC 1 cut(s) 378
SlaI CTCGAG 1 cut(s) 668
SmiMI CAYNNNNRTG 1 cut(s) 140
SmlI CTYRAG 2 cut(s) 80, 668
SmoI CTYRAG 2 cut(s) 80, 668
SpeI ACTAGT 1 cut(s) 697
SsiI CCGC 1 cut(s) 534
SspMI CTAG 3 cut(s) 224, 452, 698
StyD4I CCNGG 5 cut(s) 425, 481, 500, 527, 600
TaaI ACNGT 3 cut(s) 121, 378, 619
TaqI TCGA 3 cut(s) 669, 713, 768
TauI GCSGC 1 cut(s) 536
Tru1I TTAA 9 cut(s) 153, 162, 192, 215, 245, 296, 549, 614, 749
Tru9I TTAA 9 cut(s) 153, 162, 192, 215, 245, 296, 549, 614, 749
TscAI CASTG 3 cut(s) 118, 134, 406
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 2 cut(s) 566, 569
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 2 cut(s) 537, 611
TspRI CASTG 3 cut(s) 118, 134, 406
VpaK11BI GGWCC 1 cut(s) 378
XapI RAATTY 2 cut(s) 552, 683
XbaI TCTAGA 1 cut(s) 223
XceI RCATGY 1 cut(s) 191
XhoI CTCGAG 1 cut(s) 668
XspI CTAG 3 cut(s) 224, 452, 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.