Rh1CG133000

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0001999
Physical Location & Seq
Forward (+)
29194060 .. 29202636
8577 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG133000.1

Sequence Viewer

Length: 1383 bp
ATGCTTCCGAATCACTTGCTCCTCGAAGAGCCTATCAGGATGGCCTCCATCCTCGAGCCTTCTAAGCCTACATTCTTTCCTGCATTGACAAAGATAGTTGGAACACTTGGTCCAAATTCCCGATCCGTTGAGACAATCTCCGATTGCCTGGAGGCAGGAATGTCTGTGGCACGGTTTGACTTCTCATGGGGCGATACTGATTTTCACCAAGAGACATTGGAAAATCTGAGGATTGCCATCAAGAGGACCAAGAAGCTGTGTGCAGTTATGCTAGACACAGTGGGTCCAGAGTTGCAGGTAGTGAATAAAACTCAGTGCCCCATTTCTCTTGAGGCAGAGAACGTGGTTGTCCTAACACCTGATCAAGATAAAGATGCCACTCCAAAACTCTTACCCATAAATTTTAGTGGGCTTGCAAAGGCCGTGCAGAAAGGTGACACCATTTTTATTGGTCAATACCTCTTTACAGGAAGTGAAACTACTTCTGTATGGCTGGAGGTCACTGAGGTGATTGGTGAGGATGTGGTTTGTCTCATAAAGAACTCTGCTATCTTGGCAGGGTCGATGTATACTCTGCATGTTTCTCAAATTCGTATTGATCAGCCTACACTGACCGATCTAGATAAGGAGGTCATAAGAACGTGGGGTGTTCGTAACAATATTGACTTCCTCTCATTGTCATACACCCGTCATGCTGAAGATGTTCGCCAGGCCCGTGAATTTCTTTCGAAGTTAGGGGACATGAACCAAACTCAGATTTTTGCTAAAATTGAAAATGTTGAGGGATTAAAGCATTTTGATGATATCTTACAAGAAGCTGATGGAATTATCCTCGCTCGCGGAAATTTGGGAATAGATGTTCCACCGGAGAAGGTGTTCTTGTTTCAAAAGGCTGCTGTTCACAAGTGCAACATGGCTGGAAAGCCAGCAGTGGTGACTCGAGTTGTAGACAGTATGACAGACAACCTAAGACCTACTCGTGCTGAAGCAACTGATGTTGCCAATATAGTACTGGATGGAAGTGACGCAATTCTTCTAGGTGCAGAGACCCTGCGGGGTTTGTACCCTGTCGAAACCATCTCCATTGTTGGAAAGATTTGTGCCGAGGCTGAGAAAGTCTTCAACCAAGATCTGTATTATAAGAGGGCTGTCAAATATATTGGAGAGCCAATGACCCACTTGGAATCTATTGCTTCCTCTGCGGTACGTGCAGCCATTAAGGTGAAGGCCTCAGTGATTATATGCTTCACTTCATCTGGAAGAGCTGCAAGGTTGATCTCAAAGTACAGACCAACAATGCCTGTGCTTTCTGTTGTCATCCCTCGGGTTAAGACAAATCAGCTCCGATGGACATTTTCTGGTGCTTATGAGGTTTGTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

460

Amino Acids

50.82

Weight (kDa)

5.91

Isoelectric Point (pI)

29.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 30 - 360 3.9e-87 Pyruvate kinase, barrel domain
PK_C PF02887 395 - 459 4.2e-14 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1140
Acc36I ACCTGC 1 cut(s) 286
AccI GTMKAC 2 cut(s) 569, 948
AccII CGCG 1 cut(s) 840
AciI CCGC 3 cut(s) 840, 1054, 1202
AclWI GGATC 1 cut(s) 117
AcsI RAATTY 5 cut(s) 115, 400, 588, 719, 844
AcuI CTGAAG 2 cut(s) 717, 1005
AfaI GTAC 4 cut(s) 1011, 1064, 1206, 1286
AfiI CCNNNNNNNGG 1 cut(s) 243
AgsI TTSAA 3 cut(s) 773, 887, 1123
AjnI CCWGG 2 cut(s) 147, 708
AleI CACNNNNGTG 1 cut(s) 506
AloI GAACNNNNNNTCC 6 cut(s) 94, 126, 843, 860, 875, 892
AluBI AGCT 4 cut(s) 256, 818, 1265, 1342
AluI AGCT 4 cut(s) 256, 818, 1265, 1342
Alw26I GTCTC 4 cut(s) 125, 206, 536, 1040
AlwI GGATC 1 cut(s) 117
Ama87I CYCGRG 3 cut(s) 53, 939, 1323
AoxI GGCC 4 cut(s) 42, 420, 711, 1227
ApeKI GCWGC 3 cut(s) 893, 1211, 1265
ApoI RAATTY 5 cut(s) 115, 400, 588, 719, 844
Asp700I GAANNNNTTC 3 cut(s) 702, 875, 1118
AspS9I GGNCC 4 cut(s) 110, 246, 284, 712
AsuHPI GGTGA 6 cut(s) 197, 446, 520, 527, 946, 1234
AsuII TTCGAA 1 cut(s) 728
AvaI CYCGRG 3 cut(s) 53, 939, 1323
AvaII GGWCC 3 cut(s) 110, 246, 284
BaeGI GKGCMC 1 cut(s) 320
BarI GAAGNNNNNNTAC 2 cut(s) 463, 495
BauI CACGAG 1 cut(s) 978
BbsI GAAGAC 1 cut(s) 1111
BbvI GCAGC 3 cut(s) 880, 1223, 1252
BccI CCATC 7 cut(s) 34, 56, 245, 815, 1010, 1085, 1341
BceAI ACGGC 1 cut(s) 407
BcgI CGANNNNNNTGC 1 cut(s) 32
BciT130I CCWGG 2 cut(s) 149, 710
BclI TGATCA 2 cut(s) 361, 598
BcoDI GTCTC 4 cut(s) 125, 206, 536, 1040
BfaI CTAG 3 cut(s) 272, 620, 1037
BfuAI ACCTGC 1 cut(s) 286
BglII AGATCT 1 cut(s) 1129
BisI GCNGC 3 cut(s) 894, 1212, 1266
BlsI GCNGC 3 cut(s) 895, 1213, 1267
BmcAI AGTACT 1 cut(s) 1011
Bme1390I CCNGG 2 cut(s) 149, 710
Bme18I GGWCC 3 cut(s) 110, 246, 284
BmeT110I CYCGRG 3 cut(s) 53, 939, 1323
BmgT120I GGNCC 4 cut(s) 110, 246, 284, 712
BmiI GGNNCC 1 cut(s) 285
BmrFI CCNGG 2 cut(s) 149, 710
BmsI GCATC 1 cut(s) 364
BpiI GAAGAC 1 cut(s) 1111
BplI GAGNNNNNCTC 2 cut(s) 122, 154
BpmI CTGGAG 2 cut(s) 170, 515
Bpu14I TTCGAA 1 cut(s) 728
BpuEI CTTGAG 1 cut(s) 350
BsaAI YACGTR 1 cut(s) 1208
BsaBI GATNNNNATC 1 cut(s) 236
BsaI GGTCTC 1 cut(s) 1040
BsaJI CCNNGG 2 cut(s) 1104, 1322
BsaWI WCCGGW 1 cut(s) 865
BsaXI ACNNNNNCTCC 2 cut(s) 860, 890
Bsc4I CCNNNNNNNGG 1 cut(s) 243
Bse1I ACTGG 1 cut(s) 1017
Bse8I GATNNNNATC 1 cut(s) 236
BseBI CCWGG 2 cut(s) 149, 710
BseDI CCNNGG 2 cut(s) 1104, 1322
BseGI GGATG 5 cut(s) 45, 48, 526, 1021, 1317
BseJI GATNNNNATC 1 cut(s) 236
BseLI CCNNNNNNNGG 1 cut(s) 243
BseMII CTCAG 6 cut(s) 218, 326, 495, 767, 1101, 1245
BseNI ACTGG 1 cut(s) 1017
BseRI GAGGAG 1 cut(s) 11
BseSI GKGCMC 1 cut(s) 320
BseXI GCAGC 3 cut(s) 880, 1223, 1252
BsgI GTGCAG 4 cut(s) 282, 446, 1062, 1230
Bsh1236I CGCG 1 cut(s) 840
BshFI GGCC 4 cut(s) 44, 422, 713, 1229
BsiHKCI CYCGRG 3 cut(s) 53, 939, 1323
BsiSI CCGG 1 cut(s) 866
BslFI GGGAC 1 cut(s) 752
BslI CCNNNNNNNGG 1 cut(s) 243
BsmAI GTCTC 4 cut(s) 125, 206, 536, 1040
BsmFI GGGAC 1 cut(s) 752
BsnI GGCC 4 cut(s) 44, 422, 713, 1229
Bso31I GGTCTC 1 cut(s) 1040
BsoBI CYCGRG 3 cut(s) 53, 939, 1323
Bsp119I TTCGAA 1 cut(s) 728
Bsp1286I GDGCHC 1 cut(s) 320
Bsp143I GATC 6 cut(s) 122, 361, 598, 616, 1129, 1275
BspACI CCGC 3 cut(s) 840, 1054, 1202
BspANI GGCC 4 cut(s) 44, 422, 713, 1229
BspCNI CTCAG 6 cut(s) 219, 325, 496, 766, 1102, 1244
BspFNI CGCG 1 cut(s) 840
BspLI GGNNCC 1 cut(s) 285
BspMI ACCTGC 1 cut(s) 286
BspPI GGATC 1 cut(s) 117
BspQI GCTCTTC 2 cut(s) 21, 1255
BspT104I TTCGAA 1 cut(s) 728
BspTNI GGTCTC 1 cut(s) 1040
BsrI ACTGG 1 cut(s) 1017
BssECI CCNNGG 2 cut(s) 1104, 1322
BssMI GATC 6 cut(s) 122, 361, 598, 616, 1129, 1275
BssNAI GTATAC 1 cut(s) 570
BssSI CACGAG 1 cut(s) 978
Bst1107I GTATAC 1 cut(s) 570
Bst2BI CACGAG 1 cut(s) 978
Bst2UI CCWGG 2 cut(s) 149, 710
Bst4CI ACNGT 3 cut(s) 174, 280, 953
Bst6I CTCTTC 2 cut(s) 21, 1255
BstBAI YACGTR 1 cut(s) 1208
BstBI TTCGAA 1 cut(s) 728
BstC8I GCNNGC 3 cut(s) 414, 838, 927
BstDEI CTNAG 8 cut(s) 63, 227, 312, 504, 753, 968, 1110, 1231
BstF5I GGATG 5 cut(s) 45, 48, 526, 1021, 1317
BstFNI CGCG 1 cut(s) 840
BstKTI GATC 6 cut(s) 125, 364, 601, 619, 1132, 1278
BstMAI GTCTC 4 cut(s) 125, 206, 536, 1040
BstMBI GATC 6 cut(s) 122, 361, 598, 616, 1129, 1275
BstMWI GCNNNNNNNGC 4 cut(s) 64, 554, 1199, 1208
BstNI CCWGG 2 cut(s) 149, 710
BstNSI RCATGY 1 cut(s) 581
BstSCI CCNGG 2 cut(s) 147, 708
BstSLI GKGCMC 1 cut(s) 320
BstUI CGCG 1 cut(s) 840
BstV1I GCAGC 3 cut(s) 880, 1223, 1252
BstV2I GAAGAC 1 cut(s) 1111
BstX2I RGATCY 1 cut(s) 1129
BstYI RGATCY 1 cut(s) 1129
BstZ17I GTATAC 1 cut(s) 570
BsuRI GGCC 4 cut(s) 44, 422, 713, 1229
BtsCI GGATG 5 cut(s) 45, 48, 526, 1021, 1317
BtsI GCAGTG 1 cut(s) 936
BtsIMutI CAGTG 6 cut(s) 285, 320, 501, 608, 936, 1239
BveI ACCTGC 1 cut(s) 286
Cac8I GCNNGC 3 cut(s) 414, 838, 927
Cfr13I GGNCC 4 cut(s) 110, 246, 284, 712
CseI GACGC 1 cut(s) 1034
Csp6I GTAC 4 cut(s) 1010, 1063, 1205, 1285
CviAII CATG 5 cut(s) 186, 578, 692, 742, 913
CviQI GTAC 4 cut(s) 1010, 1063, 1205, 1285
DdeI CTNAG 8 cut(s) 63, 227, 312, 504, 753, 968, 1110, 1231
DpnI GATC 6 cut(s) 124, 363, 600, 618, 1131, 1277
DpnII GATC 6 cut(s) 122, 361, 598, 616, 1129, 1275
Eam1104I CTCTTC 2 cut(s) 21, 1255
EarI CTCTTC 2 cut(s) 21, 1255
Eco147I AGGCCT 1 cut(s) 1229
Eco31I GGTCTC 1 cut(s) 1040
Eco32I GATATC 1 cut(s) 805
Eco47I GGWCC 3 cut(s) 110, 246, 284
Eco57I CTGAAG 2 cut(s) 717, 1005
Eco88I CYCGRG 3 cut(s) 53, 939, 1323
EcoRII CCWGG 2 cut(s) 147, 708
EcoRV GATATC 1 cut(s) 805
FaeI CATG 5 cut(s) 189, 581, 695, 745, 916
FalI AAGNNNNNCTT 2 cut(s) 863, 895
FaqI GGGAC 1 cut(s) 752
FatI CATG 5 cut(s) 185, 577, 691, 741, 912
FauI CCCGC 1 cut(s) 1047
FbaI TGATCA 2 cut(s) 361, 598
FblI GTMKAC 2 cut(s) 569, 948
Fnu4HI GCNGC 3 cut(s) 894, 1212, 1266
FokI GGATG 5 cut(s) 35, 52, 533, 1028, 1304
Fsp4HI GCNGC 3 cut(s) 894, 1212, 1266
FspBI CTAG 3 cut(s) 272, 620, 1037
GluI GCNGC 3 cut(s) 894, 1212, 1266
GsuI CTGGAG 2 cut(s) 170, 515
HaeIII GGCC 4 cut(s) 44, 422, 713, 1229
HapII CCGG 1 cut(s) 866
HgaI GACGC 1 cut(s) 1034
Hin1II CATG 5 cut(s) 189, 581, 695, 745, 916
HinfI GANTC 3 cut(s) 10, 937, 1184
HpaII CCGG 1 cut(s) 866
HphI GGTGA 6 cut(s) 197, 446, 520, 527, 946, 1234
Hpy166II GTNNAC 3 cut(s) 570, 901, 949
Hpy188I TCNGA 5 cut(s) 9, 142, 228, 756, 1346
Hpy188III TCNNGA 8 cut(s) 37, 120, 241, 287, 329, 365, 620, 1257
Hpy8I GTNNAC 3 cut(s) 570, 901, 949
HpyAV CCTTC 3 cut(s) 69, 865, 1219
HpyCH4III ACNGT 3 cut(s) 174, 280, 953
HpyCH4IV ACGT 3 cut(s) 342, 641, 1207
HpyF10VI GCNNNNNNNGC 4 cut(s) 64, 554, 1199, 1208
HpyF3I CTNAG 8 cut(s) 63, 227, 312, 504, 753, 968, 1110, 1231
HpySE526I ACGT 3 cut(s) 342, 641, 1207
Hsp92II CATG 5 cut(s) 189, 581, 695, 745, 916
Ksp22I TGATCA 2 cut(s) 361, 598
Kzo9I GATC 6 cut(s) 122, 361, 598, 616, 1129, 1275
LguI GCTCTTC 2 cut(s) 21, 1255
LmnI GCTCC 2 cut(s) 24, 1347
Lsp1109I GCAGC 3 cut(s) 880, 1223, 1252
LweI GCATC 1 cut(s) 364
MaeI CTAG 3 cut(s) 272, 620, 1037
MaeII ACGT 3 cut(s) 342, 641, 1207
MaeIII GTNAC 6 cut(s) 434, 499, 653, 934, 1022, 1376
MalI GATC 6 cut(s) 124, 363, 600, 618, 1131, 1277
MboI GATC 6 cut(s) 122, 361, 598, 616, 1129, 1275
MboII GAAGA 5 cut(s) 38, 710, 1025, 1111, 1272
MflI RGATCY 1 cut(s) 1129
MhlI GDGCHC 1 cut(s) 320
MluCI AATT 8 cut(s) 115, 400, 588, 719, 768, 825, 844, 1029
MlyI GAGTC 1 cut(s) 931
MmeI TCCRAC 2 cut(s) 79, 1069
MroXI GAANNNNTTC 3 cut(s) 702, 875, 1118
MseI TTAA 3 cut(s) 788, 1218, 1329
MslI CAYNNNNRTG 3 cut(s) 506, 798, 1220
MspI CCGG 1 cut(s) 866
MspR9I CCNGG 2 cut(s) 149, 710
MvaI CCWGG 2 cut(s) 149, 710
MvnI CGCG 1 cut(s) 840
MwoI GCNNNNNNNGC 4 cut(s) 64, 554, 1199, 1208
NdeII GATC 6 cut(s) 122, 361, 598, 616, 1129, 1275
NlaIII CATG 5 cut(s) 189, 581, 695, 745, 916
NlaIV GGNNCC 1 cut(s) 285
NmeAIII GCCGAG 1 cut(s) 1129
NmuCI GTSAC 4 cut(s) 434, 499, 934, 1022
NspI RCATGY 1 cut(s) 581
NspV TTCGAA 1 cut(s) 728
OliI CACNNNNGTG 1 cut(s) 506
PaeR7I CTCGAG 2 cut(s) 53, 939
PceI AGGCCT 1 cut(s) 1229
PciSI GCTCTTC 2 cut(s) 21, 1255
PcsI WCGNNNNNNNCGW 1 cut(s) 712
PdmI GAANNNNTTC 3 cut(s) 702, 875, 1118
PfeI GAWTC 2 cut(s) 10, 1184
PkrI GCNGC 3 cut(s) 895, 1213, 1267
PleI GAGTC 1 cut(s) 931
PpsI GAGTC 1 cut(s) 931
Ppu21I YACGTR 1 cut(s) 1208
PsiI TTATAA 1 cut(s) 1140
Psp6I CCWGG 2 cut(s) 147, 708
PspGI CCWGG 2 cut(s) 147, 708
PspN4I GGNNCC 1 cut(s) 285
PspPI GGNCC 4 cut(s) 110, 246, 284, 712
PspXI VCTCGAGB 2 cut(s) 53, 939
PsuI RGATCY 1 cut(s) 1129
RsaI GTAC 4 cut(s) 1011, 1064, 1206, 1286
RsaNI GTAC 4 cut(s) 1010, 1063, 1205, 1285
RseI CAYNNNNRTG 3 cut(s) 506, 798, 1220
SapI GCTCTTC 2 cut(s) 21, 1255
SaqAI TTAA 3 cut(s) 788, 1218, 1329
SatI GCNGC 3 cut(s) 894, 1212, 1266
Sau3AI GATC 6 cut(s) 122, 361, 598, 616, 1129, 1275
Sau96I GGNCC 4 cut(s) 110, 246, 284, 712
ScaI AGTACT 1 cut(s) 1011
SchI GAGTC 1 cut(s) 931
ScrFI CCNGG 2 cut(s) 149, 710
SduI GDGCHC 1 cut(s) 320
SfaNI GCATC 1 cut(s) 364
Sfr274I CTCGAG 2 cut(s) 53, 939
SfuI TTCGAA 1 cut(s) 728
SinI GGWCC 3 cut(s) 110, 246, 284
SlaI CTCGAG 2 cut(s) 53, 939
SmiMI CAYNNNNRTG 3 cut(s) 506, 798, 1220
SmlI CTYRAG 3 cut(s) 53, 329, 939
SmoI CTYRAG 3 cut(s) 53, 329, 939
Sse9I AATT 8 cut(s) 115, 400, 588, 719, 768, 825, 844, 1029
SseBI AGGCCT 1 cut(s) 1229
SsiI CCGC 3 cut(s) 840, 1054, 1202
SspI AATATT 1 cut(s) 661
SspMI CTAG 3 cut(s) 272, 620, 1037
StuI AGGCCT 1 cut(s) 1229
StyD4I CCNGG 2 cut(s) 147, 708
TaaI ACNGT 3 cut(s) 174, 280, 953
TaiI ACGT 3 cut(s) 345, 644, 1210
TaqI TCGA 6 cut(s) 24, 54, 563, 728, 940, 1071
TaqII GACCGA 1 cut(s) 629
TasI AATT 8 cut(s) 115, 400, 588, 719, 768, 825, 844, 1029
TatI WGTACW 2 cut(s) 1009, 1284
TfiI GAWTC 2 cut(s) 10, 1184
Tru1I TTAA 3 cut(s) 788, 1218, 1329
Tru9I TTAA 3 cut(s) 788, 1218, 1329
TscAI CASTG 6 cut(s) 285, 320, 508, 615, 936, 1239
TseFI GTSAC 4 cut(s) 434, 499, 934, 1022
TseI GCWGC 3 cut(s) 893, 1211, 1265
Tsp45I GTSAC 4 cut(s) 434, 499, 934, 1022
TspDTI ATGAA 2 cut(s) 758, 1242
TspGWI ACGGA 1 cut(s) 115
TspRI CASTG 6 cut(s) 285, 320, 508, 615, 936, 1239
VpaK11BI GGWCC 3 cut(s) 110, 246, 284
XapI RAATTY 5 cut(s) 115, 400, 588, 719, 844
XbaI TCTAGA 1 cut(s) 619
XceI RCATGY 1 cut(s) 581
XcmI CCANNNNNNNNNTGG 1 cut(s) 1009
XhoI CTCGAG 2 cut(s) 53, 939
XmiI GTMKAC 2 cut(s) 569, 948
XmnI GAANNNNTTC 3 cut(s) 702, 875, 1118
XspI CTAG 3 cut(s) 272, 620, 1037
ZrmI AGTACT 1 cut(s) 1011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.