Rh3AG170600

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0001999
Physical Location & Seq
Reverse (-)
15795104 .. 15801792
6689 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG170600.1

Sequence Viewer

Length: 1218 bp
ATGCATTCGAATCATCTGCTTCTGGAGGAACCGATAAGGATGGCTTCGATACTGGAGCCGTCGAAGGCGAGTTTTTTCCCTGCAATGACGAAGATTGTGGGGACGCTGGGGCCGAAATCCCGATCTGCGGAGGTCATCTCTTCTTGCCTCAATGCTGGAATGTCTGTGGCCCGATTCGATTTCTCGTGGGGCAACCCGGAGTATCACCAGGAGACTTTGGACAATCTCAAGGCGGCTGTTAAGAGTACCAAGAAGCTTTGCGCGGTTATGTTGGACACTGTAGGTCCCGAATTGCAGGTTGTAAACAGAGGCGAGAAGATCATTGAACTCAAGGGAGATGGCTTGGTTATTCTTACCCCTGATCAGGGACAAGAACCATCTTCGGAGCTTTTGCCCATCAATTTTGATGGATTGTCAAAGGCAGTGACAAAGGGAGATACCGTTTTCATAGGCCAGTACCTGTTCACTGGAAGTGAAACTACTTCTGCATGGTTGGAGGTGTCTGAAGTGAAAGGAGATGATGTTGTTTGTGTGGTAAAGAATTCTGCAACATTGGCCGGTTCTTTGTTCACTTTGCATATCTCTCAAGTTCATATTGACTTGCCTACTCTCTCTGATAAAGATAAGCAGGTTATCAGTAGTTGGGGACTTAAAAACAAAATTGATTTCCTCTCACTGTCATATACACGACATGCAGAAGATGTTCGCCAAGCCCGCGAGTTCCTTTCTAAGTTGGGTGACCTCAGTCAGACACAAATTTTCGCTAAGATTGAAAATATGGAGGGATTAACCCATTTTGACGAGATTCTACAAGAAGCTGACGGTATTATCCTATCCCGTGGAAATTTGGGCATTGACCTGCCACCTGAGAAGGTGTTTTTATTTCAAAAGGCTGCCCTTTACAAGTGTAACATGGCTGGAAAGCCTGCTGTGGTTACTCGTGTTGTGGATAGTATGACCGACAACTTGAGGCCAACTCGTGCAGAAGCAACTGATGTTGCAAATGCTGTATTAGACGGAAGCGATGCAATTCTTCTCGGTGCTGAGACTCTTCGTGGGTTATACCCTGTTGATACCATCTCTACTGTTGGCAAAATTTGTGCTGAGGCAGAGAAGGTTTTTAACCAGGACTTGTATTTCAAGAGGACAGTCAAATATGTTGGAGAGCCAATGACACACTTGGAATCTATTGCATCCTCTGCGGTATTGACTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

405

Amino Acids

44.17

Weight (kDa)

5.24

Isoelectric Point (pI)

33.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 30 - 360 1.2e-87 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1216
Acc36I ACCTGC 3 cut(s) 286, 619, 867
AccII CGCG 2 cut(s) 263, 717
AciI CCGC 5 cut(s) 128, 233, 263, 715, 1202
AcoI YGGCCR 1 cut(s) 555
AcsI RAATTY 4 cut(s) 541, 756, 844, 1095
AcuI CTGAAG 1 cut(s) 525
AfaI GTAC 2 cut(s) 247, 458
AfiI CCNNNNNNNGG 3 cut(s) 127, 364, 365
AgsI TTSAA 4 cut(s) 326, 773, 887, 1141
AjnI CCWGG 2 cut(s) 207, 1125
AluBI AGCT 3 cut(s) 256, 388, 818
AluI AGCT 3 cut(s) 256, 388, 818
Alw26I GTCTC 2 cut(s) 206, 1040
AlwNI CAGNNNCTG 1 cut(s) 460
AoxI GGCC 5 cut(s) 110, 168, 451, 555, 971
ApeKI GCWGC 1 cut(s) 893
ApoI RAATTY 4 cut(s) 541, 756, 844, 1095
Asp700I GAANNNNTTC 1 cut(s) 702
AspLEI GCGC 1 cut(s) 263
AspS9I GGNCC 3 cut(s) 110, 169, 284
AsuC2I CCSGG 1 cut(s) 197
AsuHPI GGTGA 2 cut(s) 197, 749
AsuII TTCGAA 1 cut(s) 8
AvaII GGWCC 1 cut(s) 284
BarI GAAGNNNNNNTAC 2 cut(s) 463, 495
BauI CACGAG 3 cut(s) 184, 939, 978
BbvCI CCTCAGC 1 cut(s) 1104
BbvI GCAGC 1 cut(s) 880
BccI CCATC 6 cut(s) 34, 332, 385, 401, 404, 1085
BceAI ACGGC 1 cut(s) 43
BcgI CGANNNNNNTGC 1 cut(s) 32
BciT130I CCWGG 2 cut(s) 209, 1127
BclI TGATCA 1 cut(s) 361
BcnI CCSGG 1 cut(s) 197
BcoDI GTCTC 2 cut(s) 206, 1040
BfmI CTRYAG 1 cut(s) 279
BfuAI ACCTGC 3 cut(s) 286, 619, 867
BisI GCNGC 2 cut(s) 234, 894
BlsI GCNGC 2 cut(s) 235, 895
Bme1390I CCNGG 3 cut(s) 197, 209, 1127
Bme18I GGWCC 1 cut(s) 284
BmgT120I GGNCC 3 cut(s) 110, 169, 284
BmiI GGNNCC 4 cut(s) 30, 57, 111, 286
BmrFI CCNGG 3 cut(s) 197, 209, 1127
BmsI GCATC 2 cut(s) 1015, 1202
BoxI GACNNNNGTC 1 cut(s) 744
BplI GAGNNNNNCTC 4 cut(s) 122, 154, 961, 993
BpmI CTGGAG 2 cut(s) 44, 74
Bpu10I CCTNAGC 1 cut(s) 1104
Bpu14I TTCGAA 1 cut(s) 8
BpuEI CTTGAG 4 cut(s) 212, 314, 570, 988
BpuMI CCSGG 1 cut(s) 197
BsaJI CCNNGG 1 cut(s) 838
BsaXI ACNNNNNCTCC 4 cut(s) 426, 456, 507, 537
Bsc4I CCNNNNNNNGG 3 cut(s) 127, 364, 365
Bse118I RCCGGY 1 cut(s) 557
Bse1I ACTGG 3 cut(s) 57, 454, 472
Bse3DI GCAATG 1 cut(s) 90
BseBI CCWGG 2 cut(s) 209, 1127
BseDI CCNNGG 1 cut(s) 838
BseGI GGATG 2 cut(s) 45, 1193
BseLI CCNNNNNNNGG 3 cut(s) 127, 364, 365
BseMI GCAATG 1 cut(s) 90
BseMII CTCAG 4 cut(s) 757, 858, 1035, 1095
BseNI ACTGG 3 cut(s) 57, 454, 472
BseXI GCAGC 1 cut(s) 880
BseYI CCCAGC 1 cut(s) 106
BsgI GTGCAG 1 cut(s) 1002
Bsh1236I CGCG 2 cut(s) 263, 717
BshFI GGCC 5 cut(s) 112, 170, 453, 557, 973
BsiSI CCGG 2 cut(s) 197, 558
BslFI GGGAC 4 cut(s) 115, 270, 381, 660
BslI CCNNNNNNNGG 3 cut(s) 127, 364, 365
BsmAI GTCTC 2 cut(s) 206, 1040
BsmFI GGGAC 4 cut(s) 115, 270, 381, 660
BsmI GAATGC 1 cut(s) 4
BsnI GGCC 5 cut(s) 112, 170, 453, 557, 973
Bsp119I TTCGAA 1 cut(s) 8
Bsp143I GATC 3 cut(s) 122, 318, 361
BspACI CCGC 5 cut(s) 128, 233, 263, 715, 1202
BspANI GGCC 5 cut(s) 112, 170, 453, 557, 973
BspCNI CTCAG 4 cut(s) 756, 859, 1036, 1096
BspFNI CGCG 2 cut(s) 263, 717
BspLI GGNNCC 4 cut(s) 30, 57, 111, 286
BspMI ACCTGC 3 cut(s) 286, 619, 867
BspT104I TTCGAA 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 90
BsrFI RCCGGY 1 cut(s) 557
BsrI ACTGG 3 cut(s) 57, 454, 472
BssAI RCCGGY 1 cut(s) 557
BssECI CCNNGG 1 cut(s) 838
BssMI GATC 3 cut(s) 122, 318, 361
BssSI CACGAG 3 cut(s) 184, 939, 978
Bst2BI CACGAG 3 cut(s) 184, 939, 978
Bst2UI CCWGG 2 cut(s) 209, 1127
Bst4CI ACNGT 6 cut(s) 280, 442, 678, 824, 1087, 1150
Bst6I CTCTTC 2 cut(s) 145, 1056
BstAPI GCANNNNNTGC 1 cut(s) 1199
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 2 cut(s) 715, 927
BstDEI CTNAG 6 cut(s) 729, 743, 765, 867, 1044, 1104
BstDSI CCRYGG 1 cut(s) 838
BstEII GGTNACC 1 cut(s) 737
BstF5I GGATG 2 cut(s) 45, 1193
BstFNI CGCG 2 cut(s) 263, 717
BstHHI GCGC 1 cut(s) 263
BstKTI GATC 3 cut(s) 125, 321, 364
BstMAI GTCTC 2 cut(s) 206, 1040
BstMBI GATC 3 cut(s) 122, 318, 361
BstMWI GCNNNNNNNGC 3 cut(s) 554, 714, 1199
BstNI CCWGG 2 cut(s) 209, 1127
BstNSI RCATGY 1 cut(s) 695
BstPAI GACNNNNGTC 1 cut(s) 744
BstPI GGTNACC 1 cut(s) 737
BstSCI CCNGG 3 cut(s) 195, 207, 1125
BstSFI CTRYAG 1 cut(s) 279
BstUI CGCG 2 cut(s) 263, 717
BstV1I GCAGC 1 cut(s) 880
BsuRI GGCC 5 cut(s) 112, 170, 453, 557, 973
BtgI CCRYGG 1 cut(s) 838
BtgZI GCGATG 1 cut(s) 1038
BtsCI GGATG 2 cut(s) 45, 1193
BtsI GCAGTG 1 cut(s) 429
BtsIMutI CAGTG 4 cut(s) 276, 429, 465, 674
BveI ACCTGC 3 cut(s) 286, 619, 867
Cac8I GCNNGC 2 cut(s) 715, 927
CaiI CAGNNNCTG 1 cut(s) 460
CfoI GCGC 1 cut(s) 263
Cfr10I RCCGGY 1 cut(s) 557
Cfr13I GGNCC 3 cut(s) 110, 169, 284
CseI GACGC 1 cut(s) 112
Csp6I GTAC 2 cut(s) 246, 457
CviAII CATG 3 cut(s) 489, 692, 913
CviQI GTAC 2 cut(s) 246, 457
DdeI CTNAG 6 cut(s) 729, 743, 765, 867, 1044, 1104
DpnI GATC 3 cut(s) 124, 320, 363
DpnII GATC 3 cut(s) 122, 318, 361
EaeI YGGCCR 1 cut(s) 555
Eam1104I CTCTTC 2 cut(s) 145, 1056
EarI CTCTTC 2 cut(s) 145, 1056
Eco47I GGWCC 1 cut(s) 284
Eco57I CTGAAG 1 cut(s) 525
Eco91I GGTNACC 1 cut(s) 737
EcoO109I RGGNCCY 1 cut(s) 284
EcoO65I GGTNACC 1 cut(s) 737
EcoRI GAATTC 1 cut(s) 541
EcoRII CCWGG 2 cut(s) 207, 1125
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 3 cut(s) 492, 695, 916
FalI AAGNNNNNCTT 2 cut(s) 28, 60
FaqI GGGAC 4 cut(s) 115, 270, 381, 660
FatI CATG 3 cut(s) 488, 691, 912
FauI CCCGC 1 cut(s) 722
FbaI TGATCA 1 cut(s) 361
Fnu4HI GCNGC 2 cut(s) 234, 894
FokI GGATG 2 cut(s) 52, 1180
Fsp4HI GCNGC 2 cut(s) 234, 894
GlaI GCGC 1 cut(s) 262
GluI GCNGC 2 cut(s) 234, 894
GsaI CCCAGC 1 cut(s) 110
GsuI CTGGAG 2 cut(s) 44, 74
HaeIII GGCC 5 cut(s) 112, 170, 453, 557, 973
HapII CCGG 2 cut(s) 197, 558
HgaI GACGC 1 cut(s) 112
HhaI GCGC 1 cut(s) 263
Hin1II CATG 3 cut(s) 492, 695, 916
Hin6I GCGC 1 cut(s) 261
HinP1I GCGC 1 cut(s) 261
HindIII AAGCTT 1 cut(s) 254
HinfI GANTC 5 cut(s) 10, 174, 805, 1048, 1184
HpaII CCGG 2 cut(s) 197, 558
HphI GGTGA 2 cut(s) 197, 749
Hpy166II GTNNAC 3 cut(s) 304, 465, 570
Hpy188I TCNGA 4 cut(s) 385, 505, 616, 750
Hpy188III TCNNGA 4 cut(s) 23, 120, 287, 1141
Hpy8I GTNNAC 3 cut(s) 304, 465, 570
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 3 cut(s) 58, 865, 1108
HpyCH4III ACNGT 6 cut(s) 280, 442, 678, 824, 1087, 1150
HpyF10VI GCNNNNNNNGC 3 cut(s) 554, 714, 1199
HpyF3I CTNAG 6 cut(s) 729, 743, 765, 867, 1044, 1104
Hsp92II CATG 3 cut(s) 492, 695, 916
HspAI GCGC 1 cut(s) 261
Ksp22I TGATCA 1 cut(s) 361
Kzo9I GATC 3 cut(s) 122, 318, 361
LmnI GCTCC 2 cut(s) 55, 385
Lsp1109I GCAGC 1 cut(s) 880
LweI GCATC 2 cut(s) 1015, 1202
MaeIII GTNAC 4 cut(s) 424, 737, 908, 934
MalI GATC 3 cut(s) 124, 320, 363
MboI GATC 3 cut(s) 122, 318, 361
MboII GAAGA 7 cut(s) 103, 132, 328, 372, 710, 1025, 1043
MluCI AATT 8 cut(s) 290, 400, 541, 660, 756, 844, 1029, 1095
MlyI GAGTC 1 cut(s) 1042
MmeI TCCRAC 3 cut(s) 252, 474, 1141
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 702
MseI TTAA 4 cut(s) 240, 651, 788, 1122
MspI CCGG 2 cut(s) 197, 558
MspR9I CCNGG 3 cut(s) 197, 209, 1127
Mva1269I GAATGC 1 cut(s) 4
MvaI CCWGG 2 cut(s) 209, 1127
MvnI CGCG 2 cut(s) 263, 717
MwoI GCNNNNNNNGC 3 cut(s) 554, 714, 1199
NciI CCSGG 1 cut(s) 197
NdeII GATC 3 cut(s) 122, 318, 361
NlaIII CATG 3 cut(s) 492, 695, 916
NlaIV GGNNCC 4 cut(s) 30, 57, 111, 286
NmuCI GTSAC 2 cut(s) 424, 737
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 695
NspV TTCGAA 1 cut(s) 8
PcsI WCGNNNNNNNCGW 1 cut(s) 110
PctI GAATGC 1 cut(s) 4
PdmI GAANNNNTTC 1 cut(s) 702
PfeI GAWTC 4 cut(s) 10, 174, 805, 1184
PkrI GCNGC 2 cut(s) 235, 895
PleI GAGTC 1 cut(s) 1042
PpsI GAGTC 1 cut(s) 1042
PpuMI RGGWCCY 1 cut(s) 284
PshAI GACNNNNGTC 1 cut(s) 744
PsiI TTATAA 1 cut(s) 1216
Psp5II RGGWCCY 1 cut(s) 284
Psp6I CCWGG 2 cut(s) 207, 1125
PspEI GGTNACC 1 cut(s) 737
PspFI CCCAGC 1 cut(s) 106
PspGI CCWGG 2 cut(s) 207, 1125
PspN4I GGNNCC 4 cut(s) 30, 57, 111, 286
PspPI GGNCC 3 cut(s) 110, 169, 284
PspPPI RGGWCCY 1 cut(s) 284
PstNI CAGNNNCTG 1 cut(s) 460
RsaI GTAC 2 cut(s) 247, 458
RsaNI GTAC 2 cut(s) 246, 457
SaqAI TTAA 4 cut(s) 240, 651, 788, 1122
SatI GCNGC 2 cut(s) 234, 894
Sau3AI GATC 3 cut(s) 122, 318, 361
Sau96I GGNCC 3 cut(s) 110, 169, 284
SchI GAGTC 1 cut(s) 1042
ScrFI CCNGG 3 cut(s) 197, 209, 1127
SfaNI GCATC 2 cut(s) 1015, 1202
SfcI CTRYAG 1 cut(s) 279
SfuI TTCGAA 1 cut(s) 8
SinI GGWCC 1 cut(s) 284
SmlI CTYRAG 4 cut(s) 227, 329, 585, 967
SmoI CTYRAG 4 cut(s) 227, 329, 585, 967
Sse9I AATT 8 cut(s) 290, 400, 541, 660, 756, 844, 1029, 1095
SsiI CCGC 5 cut(s) 128, 233, 263, 715, 1202
StyD4I CCNGG 3 cut(s) 195, 207, 1125
TaaI ACNGT 6 cut(s) 280, 442, 678, 824, 1087, 1150
TaqI TCGA 4 cut(s) 8, 47, 62, 177
TaqII GACCGA 1 cut(s) 974
TasI AATT 8 cut(s) 290, 400, 541, 660, 756, 844, 1029, 1095
TauI GCSGC 1 cut(s) 236
TfiI GAWTC 4 cut(s) 10, 174, 805, 1184
Tru1I TTAA 4 cut(s) 240, 651, 788, 1122
Tru9I TTAA 4 cut(s) 240, 651, 788, 1122
TscAI CASTG 4 cut(s) 283, 429, 472, 681
TseFI GTSAC 2 cut(s) 424, 737
TseI GCWGC 1 cut(s) 893
Tsp45I GTSAC 2 cut(s) 424, 737
TspDTI ATGAA 2 cut(s) 436, 581
TspGWI ACGGA 1 cut(s) 1032
TspRI CASTG 4 cut(s) 283, 429, 472, 681
VpaK11BI GGWCC 1 cut(s) 284
XapI RAATTY 4 cut(s) 541, 756, 844, 1095
XceI RCATGY 1 cut(s) 695
XmnI GAANNNNTTC 1 cut(s) 702
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.