MD02G1244000.v1.1

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001999
Physical Location & Seq
Reverse (-)
29398666 .. 29407548
8883 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1244000.v1.1.491

Sequence Viewer

Length: 1584 bp
ATGCATTCGAGTCACTTGCTTCTGGAAGAGCCAATCAGGATGGCTTCGATTCTTGAGCCTTCCAATTCTACATTCTTTCCTGCAATGACAAGGATTGTGGGAACACTTGGTCCAAATTCACGATCCGTTGAGACAATCTCTGGTTGCTTGAAGGCAGGAATGTCTGTGGCACGTTTTGATTTTTCATGGGGCGATGCTGAGTTTCACCAAGAGACGTTGGGAAACCTGAAGGTTGCAATCAAGAGTACCAATAAACTTTGTGCAGTAATGCTAGACACAGTGGGTCCAGAGTTGCAGGTAGTGAATAAAAGTGAACATCCCATATCTCTCCAAGAAGATACCCTGGTTGTCCTGACACCTGATCAAGACAAAGAGGCCACCTCAAATCTACTACCCATAAATTTTAGTGGCCTGGCAAAGGCGGTGAAGAAGGGAGATACAATTTTTATTGGTCAATACCTCTTTACGGGAAGTGAAACTACTTCTGTGTGGCTGGAGGTCACCGAGGTGAATGGTGAAGATGTGGTTTGTCTCATAAAGAATTCTGCTACCTTGGCAGGGCCGTTGTATACTTTGCATGTCTCTCAGATTCATATTGATCTGCCTACACTTACCGATAAAGATAAGGAGGTCATAAGAACTTGGGGTGTTTGTAACAACATCGACTTCCTCTCATTATCATATACGCGTCATGCTCAAGATGTTCGCCATGCCCGTGAGTTTCTTTCAAAGTTAGGTGACCTCAAGCAAACTCAGGTTTTTGCAAAAATTGAAAATGTTGAGGGATTACACCATTTTGATGAAATCTTGCAAGAAGCTGATGGTATTATCCTCTCTCGTGGAAATCTGGGGATAGATCTCCCACCAGAGAAGGTATTTTTATTTCAAAAGGCTGCTGTTTACAAGTGCAACATGGCTGGAAAGCCAGCAGTGGTGACTCGGGTTGTGGACAGTATGACTGACAACCTGAGACCTACTCGTGCGGAAGCAACTGATGTTGCCAATGCTGTACTGGATGGAACTGATGCAATTGTTCTAGGTGCAGAGACCCTGCGCGGTTTGTACCCTGTTGAGACGATCTCCATTGTTGGAAAGATTTGCTCTGAGGCGGAGAAAGTTTTCAATCAAGATTTGTATTTTAAGAGGGCTGTCAAATATGTTGGAGAACCAATGACTCACTTGGAATCTATTACTTCCTCTGCGGTACGTGCAGCTATCAAGGTGAAGGCCTCTCTCATTATTTGCTTCACTTCATCTGGAAGAGCAGCAAGGCTGATTGCAAAGTACAGACCAACAATGCCTGTCCTTTCTGTTGTCATTCCTCGGGTCCAGACAAATCAACTCCGATGGAGATTTTCTGGTGCATTTGAGGCAAGGCAATCACTCATTGTTAGAGGCCTGTTTCCCATGCTAGCAGATCCTCGACATCCAGCTGAAGCTACAGGTGCAACAAATGAGTCGATTCTGAAAATCGCTTTGGATCATGGCAGGGTCGCTGGTGTCATAAAATCGCATGATCGTGTCGTAATTTGCCAGAAAGTTGGCGATACTTCTGTGATCAAGATCATCGAGCTTGAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

528

Amino Acids

57.79

Weight (kDa)

6.3

Isoelectric Point (pI)

28.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 30 - 360 2e-87 Pyruvate kinase, barrel domain
PK_C PF02887 395 - 523 4.3e-21 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 286
AccI GTMKAC 1 cut(s) 569
AccII CGCG 2 cut(s) 688, 1056
AciI CCGC 5 cut(s) 422, 983, 1056, 1109, 1202
AclWI GGATC 3 cut(s) 117, 1412, 1488
AcsI RAATTY 3 cut(s) 115, 400, 541
AcuI CTGAAG 2 cut(s) 248, 1455
AfaI GTAC 5 cut(s) 247, 1011, 1064, 1206, 1286
AfiI CCNNNNNNNGG 3 cut(s) 418, 466, 558
AflIII ACRYGT 1 cut(s) 686
AgsI TTSAA 6 cut(s) 151, 729, 773, 887, 1123, 1577
AjnI CCWGG 2 cut(s) 342, 411
AjuI GAANNNNNNNTTGG 4 cut(s) 56, 88, 1460, 1492
AleI CACNNNNGTG 1 cut(s) 506
AloI GAACNNNNNNTCC 2 cut(s) 94, 126
AluBI AGCT 5 cut(s) 818, 1214, 1433, 1439, 1573
AluI AGCT 5 cut(s) 818, 1214, 1433, 1439, 1573
Alw26I GTCTC 7 cut(s) 125, 206, 536, 586, 964, 1040, 1067
AlwI GGATC 3 cut(s) 117, 1412, 1488
Ama87I CYCGRG 2 cut(s) 939, 1323
AoxI GGCC 5 cut(s) 375, 409, 560, 1227, 1396
ApeKI GCWGC 3 cut(s) 893, 1211, 1265
ApoI RAATTY 3 cut(s) 115, 400, 541
Asp700I GAANNNNTTC 1 cut(s) 1118
AspLEI GCGC 1 cut(s) 1056
AspS9I GGNCC 4 cut(s) 110, 284, 560, 1327
AsuHPI GGTGA 8 cut(s) 197, 436, 493, 520, 527, 749, 946, 1234
AsuNHI GCTAGC 1 cut(s) 1411
AvaI CYCGRG 2 cut(s) 939, 1323
AvaII GGWCC 3 cut(s) 110, 284, 1327
BaeI ACNNNNGTAYC 4 cut(s) 330, 363, 1539, 1572
BarI GAAGNNNNNNTAC 2 cut(s) 463, 495
BauI CACGAG 2 cut(s) 837, 978
BbvI GCAGC 3 cut(s) 880, 1223, 1277
BccI CCATC 4 cut(s) 34, 815, 1010, 1341
BceAI ACGGC 1 cut(s) 547
BcgI CGANNNNNNTGC 1 cut(s) 32
BciT130I CCWGG 2 cut(s) 344, 413
BclI TGATCA 2 cut(s) 361, 1557
BcoDI GTCTC 7 cut(s) 125, 206, 536, 586, 964, 1040, 1067
BfaI CTAG 3 cut(s) 272, 1037, 1412
BfmI CTRYAG 1 cut(s) 1440
BfuAI ACCTGC 1 cut(s) 286
BglII AGATCT 1 cut(s) 856
BisI GCNGC 3 cut(s) 894, 1212, 1266
BlsI GCNGC 3 cut(s) 895, 1213, 1267
Bme1390I CCNGG 2 cut(s) 344, 413
Bme18I GGWCC 3 cut(s) 110, 284, 1327
BmeT110I CYCGRG 2 cut(s) 939, 1323
BmgT120I GGNCC 4 cut(s) 110, 284, 560, 1327
BmiI GGNNCC 2 cut(s) 285, 1328
BmrFI CCNGG 2 cut(s) 344, 413
BmsI GCATC 2 cut(s) 184, 1015
BmtI GCTAGC 1 cut(s) 1415
BplI GAGNNNNNCTC 8 cut(s) 122, 154, 365, 397, 961, 993, 1064, 1096
BpmI CTGGAG 1 cut(s) 515
BpuEI CTTGAG 3 cut(s) 74, 681, 728
BsaAI YACGTR 1 cut(s) 1208
BsaBI GATNNNNATC 1 cut(s) 1562
BsaI GGTCTC 2 cut(s) 964, 1040
BsaJI CCNNGG 4 cut(s) 342, 504, 552, 1322
Bsc4I CCNNNNNNNGG 3 cut(s) 418, 466, 558
Bse1I ACTGG 1 cut(s) 1017
Bse3DI GCAATG 1 cut(s) 90
Bse8I GATNNNNATC 1 cut(s) 1562
BseBI CCWGG 2 cut(s) 344, 413
BseDI CCNNGG 4 cut(s) 342, 504, 552, 1322
BseGI GGATG 4 cut(s) 45, 316, 1021, 1426
BseJI GATNNNNATC 1 cut(s) 1562
BseLI CCNNNNNNNGG 3 cut(s) 418, 466, 558
BseMI GCAATG 1 cut(s) 90
BseMII CTCAG 5 cut(s) 189, 599, 767, 959, 1095
BseNI ACTGG 1 cut(s) 1017
BseXI GCAGC 3 cut(s) 880, 1223, 1277
BsgI GTGCAG 3 cut(s) 282, 1062, 1230
Bsh1236I CGCG 2 cut(s) 688, 1056
BshFI GGCC 5 cut(s) 377, 411, 562, 1229, 1398
BsiHKCI CYCGRG 2 cut(s) 939, 1323
BslI CCNNNNNNNGG 3 cut(s) 418, 466, 558
BsmAI GTCTC 7 cut(s) 125, 206, 536, 586, 964, 1040, 1067
BsmBI CGTCTC 2 cut(s) 206, 1067
BsmI GAATGC 1 cut(s) 4
BsnI GGCC 5 cut(s) 377, 411, 562, 1229, 1398
Bso31I GGTCTC 2 cut(s) 964, 1040
BsoBI CYCGRG 2 cut(s) 939, 1323
BspACI CCGC 5 cut(s) 422, 983, 1056, 1109, 1202
BspANI GGCC 5 cut(s) 377, 411, 562, 1229, 1398
BspCNI CTCAG 5 cut(s) 190, 598, 766, 960, 1096
BspFNI CGCG 2 cut(s) 688, 1056
BspLI GGNNCC 2 cut(s) 285, 1328
BspMI ACCTGC 1 cut(s) 286
BspOI GCTAGC 1 cut(s) 1415
BspPI GGATC 3 cut(s) 117, 1412, 1488
BspQI GCTCTTC 2 cut(s) 21, 1255
BspTNI GGTCTC 2 cut(s) 964, 1040
BsrDI GCAATG 1 cut(s) 90
BsrI ACTGG 1 cut(s) 1017
BssECI CCNNGG 4 cut(s) 342, 504, 552, 1322
BssNAI GTATAC 1 cut(s) 570
BssSI CACGAG 2 cut(s) 837, 978
BssT1I CCWWGG 1 cut(s) 552
Bst1107I GTATAC 1 cut(s) 570
Bst2BI CACGAG 2 cut(s) 837, 978
Bst2UI CCWGG 2 cut(s) 344, 413
Bst4CI ACNGT 2 cut(s) 280, 953
Bst6I CTCTTC 2 cut(s) 21, 1255
BstBAI YACGTR 1 cut(s) 1208
BstC8I GCNNGC 2 cut(s) 927, 1413
BstDEI CTNAG 5 cut(s) 198, 585, 753, 968, 1104
BstEII GGTNACC 2 cut(s) 499, 737
BstENI CCTNNNNNAGG 1 cut(s) 416
BstF5I GGATG 4 cut(s) 45, 316, 1021, 1426
BstFNI CGCG 2 cut(s) 688, 1056
BstHHI GCGC 1 cut(s) 1056
BstMAI GTCTC 7 cut(s) 125, 206, 536, 586, 964, 1040, 1067
BstMWI GCNNNNNNNGC 4 cut(s) 554, 1208, 1370, 1445
BstNI CCWGG 2 cut(s) 344, 413
BstNSI RCATGY 1 cut(s) 581
BstPI GGTNACC 2 cut(s) 499, 737
BstSCI CCNGG 2 cut(s) 342, 411
BstSFI CTRYAG 1 cut(s) 1440
BstUI CGCG 2 cut(s) 688, 1056
BstV1I GCAGC 3 cut(s) 880, 1223, 1277
BstX2I RGATCY 2 cut(s) 856, 1417
BstXI CCANNNNNNTGG 1 cut(s) 1541
BstYI RGATCY 2 cut(s) 856, 1417
BstZ17I GTATAC 1 cut(s) 570
BsuRI GGCC 5 cut(s) 377, 411, 562, 1229, 1398
BtgZI GCGATG 1 cut(s) 207
BtsCI GGATG 4 cut(s) 45, 316, 1021, 1426
BtsI GCAGTG 1 cut(s) 936
BtsIMutI CAGTG 2 cut(s) 285, 936
BveI ACCTGC 1 cut(s) 286
Cac8I GCNNGC 2 cut(s) 927, 1413
CfoI GCGC 1 cut(s) 1056
Cfr13I GGNCC 4 cut(s) 110, 284, 560, 1327
CseI GACGC 1 cut(s) 677
Csp6I GTAC 5 cut(s) 246, 1010, 1063, 1205, 1285
CspCI CAANNNNNGTGG 2 cut(s) 78, 113
CviAII CATG 8 cut(s) 186, 578, 692, 710, 913, 1408, 1484, 1514
CviQI GTAC 5 cut(s) 246, 1010, 1063, 1205, 1285
DdeI CTNAG 5 cut(s) 198, 585, 753, 968, 1104
Eam1104I CTCTTC 2 cut(s) 21, 1255
EarI CTCTTC 2 cut(s) 21, 1255
EciI GGCGGA 1 cut(s) 1124
Eco130I CCWWGG 1 cut(s) 552
Eco147I AGGCCT 2 cut(s) 1229, 1398
Eco31I GGTCTC 2 cut(s) 964, 1040
Eco47I GGWCC 3 cut(s) 110, 284, 1327
Eco57I CTGAAG 2 cut(s) 248, 1455
Eco88I CYCGRG 2 cut(s) 939, 1323
Eco91I GGTNACC 2 cut(s) 499, 737
EcoNI CCTNNNNNAGG 1 cut(s) 416
EcoO65I GGTNACC 2 cut(s) 499, 737
EcoRI GAATTC 1 cut(s) 541
EcoRII CCWGG 2 cut(s) 342, 411
EcoT14I CCWWGG 1 cut(s) 552
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 552
Esp3I CGTCTC 2 cut(s) 206, 1067
FaeI CATG 8 cut(s) 189, 581, 695, 713, 916, 1411, 1487, 1517
FatI CATG 8 cut(s) 185, 577, 691, 709, 912, 1407, 1483, 1513
FbaI TGATCA 2 cut(s) 361, 1557
FblI GTMKAC 1 cut(s) 569
Fnu4HI GCNGC 3 cut(s) 894, 1212, 1266
FokI GGATG 4 cut(s) 52, 303, 1028, 1413
Fsp4HI GCNGC 3 cut(s) 894, 1212, 1266
FspBI CTAG 3 cut(s) 272, 1037, 1412
GlaI GCGC 1 cut(s) 1055
GluI GCNGC 3 cut(s) 894, 1212, 1266
GsuI CTGGAG 1 cut(s) 515
HaeIII GGCC 5 cut(s) 377, 411, 562, 1229, 1398
HgaI GACGC 1 cut(s) 677
HhaI GCGC 1 cut(s) 1056
Hin1II CATG 8 cut(s) 189, 581, 695, 713, 916, 1411, 1487, 1517
Hin6I GCGC 1 cut(s) 1054
HinP1I GCGC 1 cut(s) 1054
HinfI GANTC 8 cut(s) 10, 49, 589, 937, 1174, 1184, 1457, 1462
HphI GGTGA 8 cut(s) 197, 436, 493, 520, 527, 749, 946, 1234
Hpy166II GTNNAC 4 cut(s) 314, 570, 901, 949
Hpy188I TCNGA 4 cut(s) 588, 1105, 1346, 1467
Hpy8I GTNNAC 4 cut(s) 314, 570, 901, 949
HpyAV CCTTC 6 cut(s) 69, 145, 223, 424, 865, 1219
HpyCH4III ACNGT 2 cut(s) 280, 953
HpyCH4IV ACGT 3 cut(s) 172, 215, 1207
HpyF10VI GCNNNNNNNGC 4 cut(s) 554, 1208, 1370, 1445
HpyF3I CTNAG 5 cut(s) 198, 585, 753, 968, 1104
HpySE526I ACGT 3 cut(s) 172, 215, 1207
Hsp92II CATG 8 cut(s) 189, 581, 695, 713, 916, 1411, 1487, 1517
HspAI GCGC 1 cut(s) 1054
Ksp22I TGATCA 2 cut(s) 361, 1557
LguI GCTCTTC 2 cut(s) 21, 1255
Lsp1109I GCAGC 3 cut(s) 880, 1223, 1277
LweI GCATC 2 cut(s) 184, 1015
MaeI CTAG 3 cut(s) 272, 1037, 1412
MaeII ACGT 3 cut(s) 172, 215, 1207
MaeIII GTNAC 5 cut(s) 11, 499, 653, 737, 934
MboII GAAGA 5 cut(s) 38, 347, 439, 530, 1272
MfeI CAATTG 1 cut(s) 1029
MflI RGATCY 2 cut(s) 856, 1417
MluCI AATT 8 cut(s) 64, 115, 400, 441, 541, 768, 1029, 1527
MluI ACGCGT 1 cut(s) 686
MlyI GAGTC 4 cut(s) 19, 931, 1168, 1466
MmeI TCCRAC 2 cut(s) 1069, 1141
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 1118
MseI TTAA 1 cut(s) 1140
MslI CAYNNNNRTG 4 cut(s) 506, 714, 798, 1518
MspA1I CMGCKG 1 cut(s) 1433
MspR9I CCNGG 2 cut(s) 344, 413
MunI CAATTG 1 cut(s) 1029
Mva1269I GAATGC 1 cut(s) 4
MvaI CCWGG 2 cut(s) 344, 413
MvnI CGCG 2 cut(s) 688, 1056
MwoI GCNNNNNNNGC 4 cut(s) 554, 1208, 1370, 1445
NheI GCTAGC 1 cut(s) 1411
NlaIII CATG 8 cut(s) 189, 581, 695, 713, 916, 1411, 1487, 1517
NlaIV GGNNCC 2 cut(s) 285, 1328
NmuCI GTSAC 4 cut(s) 11, 499, 737, 934
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 581
OliI CACNNNNGTG 1 cut(s) 506
PceI AGGCCT 2 cut(s) 1229, 1398
PciSI GCTCTTC 2 cut(s) 21, 1255
PcsI WCGNNNNNNNCGW 1 cut(s) 712
PctI GAATGC 1 cut(s) 4
PdmI GAANNNNTTC 1 cut(s) 1118
PfeI GAWTC 4 cut(s) 49, 589, 1184, 1462
PkrI GCNGC 3 cut(s) 895, 1213, 1267
PleI GAGTC 4 cut(s) 18, 931, 1168, 1465
PpsI GAGTC 4 cut(s) 18, 931, 1168, 1465
Ppu21I YACGTR 1 cut(s) 1208
Psp6I CCWGG 2 cut(s) 342, 411
PspEI GGTNACC 2 cut(s) 499, 737
PspGI CCWGG 2 cut(s) 342, 411
PspN4I GGNNCC 2 cut(s) 285, 1328
PspPI GGNCC 4 cut(s) 110, 284, 560, 1327
PsuI RGATCY 2 cut(s) 856, 1417
PvuII CAGCTG 1 cut(s) 1433
RsaI GTAC 5 cut(s) 247, 1011, 1064, 1206, 1286
RsaNI GTAC 5 cut(s) 246, 1010, 1063, 1205, 1285
RseI CAYNNNNRTG 4 cut(s) 506, 714, 798, 1518
SapI GCTCTTC 2 cut(s) 21, 1255
SaqAI TTAA 1 cut(s) 1140
SatI GCNGC 3 cut(s) 894, 1212, 1266
Sau96I GGNCC 4 cut(s) 110, 284, 560, 1327
SchI GAGTC 4 cut(s) 19, 931, 1168, 1466
ScrFI CCNGG 2 cut(s) 344, 413
SfaNI GCATC 2 cut(s) 184, 1015
SfcI CTRYAG 1 cut(s) 1440
SinI GGWCC 3 cut(s) 110, 284, 1327
SmiMI CAYNNNNRTG 4 cut(s) 506, 714, 798, 1518
SmlI CTYRAG 3 cut(s) 53, 696, 743
SmoI CTYRAG 3 cut(s) 53, 696, 743
Sse9I AATT 8 cut(s) 64, 115, 400, 441, 541, 768, 1029, 1527
SseBI AGGCCT 2 cut(s) 1229, 1398
SsiI CCGC 5 cut(s) 422, 983, 1056, 1109, 1202
SspMI CTAG 3 cut(s) 272, 1037, 1412
StuI AGGCCT 2 cut(s) 1229, 1398
StyD4I CCNGG 2 cut(s) 342, 411
StyI CCWWGG 1 cut(s) 552
TaaI ACNGT 2 cut(s) 280, 953
TaiI ACGT 3 cut(s) 175, 218, 1210
TaqI TCGA 6 cut(s) 8, 47, 663, 1423, 1460, 1569
TasI AATT 8 cut(s) 64, 115, 400, 441, 541, 768, 1029, 1527
TatI WGTACW 2 cut(s) 1009, 1284
TfiI GAWTC 4 cut(s) 49, 589, 1184, 1462
Tru1I TTAA 1 cut(s) 1140
Tru9I TTAA 1 cut(s) 1140
TscAI CASTG 2 cut(s) 285, 936
TseFI GTSAC 4 cut(s) 11, 499, 737, 934
TseI GCWGC 3 cut(s) 893, 1211, 1265
Tsp45I GTSAC 4 cut(s) 11, 499, 737, 934
TspDTI ATGAA 4 cut(s) 174, 581, 816, 1242
TspGWI ACGGA 1 cut(s) 115
TspRI CASTG 2 cut(s) 285, 936
VpaK11BI GGWCC 3 cut(s) 110, 284, 1327
XagI CCTNNNNNAGG 1 cut(s) 416
XapI RAATTY 3 cut(s) 115, 400, 541
XceI RCATGY 1 cut(s) 581
XcmI CCANNNNNNNNNTGG 1 cut(s) 1009
XmiI GTMKAC 1 cut(s) 569
XmnI GAANNNNTTC 1 cut(s) 1118
XspI CTAG 3 cut(s) 272, 1037, 1412
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.