Rroxscaffold_4G00316390

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001999
Physical Location & Seq
Reverse (-)
41217921 .. 41226857
8937 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00316390.1

Sequence Viewer

Length: 1581 bp
ATGCTTCCGAATCACTTGCTCCTCCAAGAGCCTATCAGAATGGCCTCCATCCTCGAGCCTTCTAAGCCTACATTCTTTCCTGCATTGACAAAGATAGTTGGAACACTTGGTCCAAATTCCCGATCCGTTGAGATAATCTCCGGTTGCCTGGAGGCAGGAATGTCTGTGGCACGGTTTGACTTCTCATGGGGCGATACTGATTTTCACCAAGAGACATTGGAAAATCTGAGGATTGCCATCAAGAGAACCAAGAAGCTGTGTGCAGTTATGCTAGACACAGTGGGTCCAGAGTTGCAGGTAGTGAATAAAACTCAGTGCCCCATTTCTCTTGAGGCAGAGAACGTGGTTGTCCTAACACCTGATCAAGACAAAGATGCCACTCCAAAACTCTTACCCATAAATTTTAGTGGGCTTGCAAAGGCCGTGCAGAAAGGTGACACCATTTTTATTGGTCAATACCTCTTTACAGGAGGTGAAACTACTTCTGTATGGCTGGAGGTCACTGAGGTGATTGGTGAGGATGTGGTTTGTCTCATAAAGAACTCTGCTATCTTGGCAGGGTCGATGTATACTCTGCATGTTTCTCAAATTCGTATTGATCAGCCTACACTAACCGATCTAGATAAGGAGGTCATAAGAACGTGGGGTGTTCAAAACAATATTGACTTCCTCTCATTGTCATACACCCGTCATGCTGAAGATGTTCGCCAGGCCCGTGAATTTCTTTCGAAGTTAGACATGAACCAAACTCAGATTTTTGCTAAAATCGAAAATGTTGAGGGATTAAAACATTTTGATGATATCTTACAAGAAGCTGATGGAATTATCCTCGCTCGCGGAAATTTGGGAATAGATGTTCCACCGGAGAAAGTGTTCTTGTTTCAAAAGGCTGCTGTTCACAAGTGCAACATGGCTGGAAAGCCAGCAGTGGTGACTCGAGTTGTAGACAGTATGACAGACAACCTAAGACCTACTCGTGCTGAAGCAACTGATGTTGCCAATATAGTACTGGATGGAAGTGACGCAATTCTTCTAGGTGCAGAGACCCTGCGGGGTTTGTACCCTGTCGAAACCATCTCCATTGTTGGAAAGATTTGTGCCGAGGCTGAGAAAGTCTTCAACCAAGACCTGTATTTTAAGAGGGCTGTCAAATATATTGGAGAGCCAATGACCCACATGGAATCTATTGCTTCCTCTGCGGTACGTGCAGCCATTAAGGTGAAGGCCTCAGTGATTATATGCTTCACTTCATCTGGAAGAGCTGCAAGGTTGATCTCAAAGTACAGACCAACAATGCCTGTGCTTTCTGTTGTCATCCCTCGGGTTAAGACAAATCAGCTCCGATGGACATTTTCTGGTGCTTATGAGGCAAGGCAATCACTTATTGTCAGAGGTCTTTTCCCAATGCTTGCAGATCCTCGACATCCCGCCGAGGCCACCGGTGCAGCAAATGAGTCAATTCTAAAGGTCGCTTTGGATTTTGGCAAAGCATCTGGTGTTATAAAGCCTCATGATCGTGTTGTAGTATGCCAGAAAGTTGCTGATGCATCTGTGGTGAAGATACTTGAGCTGGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

526

Amino Acids

57.75

Weight (kDa)

6.21

Isoelectric Point (pI)

28.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 30 - 359 1.1e-86 Pyruvate kinase, barrel domain
PK_C PF02887 394 - 514 1.1e-21 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1502
Acc36I ACCTGC 1 cut(s) 286
AccI GTMKAC 2 cut(s) 569, 945
AccII CGCG 1 cut(s) 837
AciI CCGC 4 cut(s) 837, 1051, 1199, 1428
AclWI GGATC 2 cut(s) 117, 1409
AcsI RAATTY 5 cut(s) 115, 400, 588, 719, 841
AcuI CTGAAG 2 cut(s) 717, 1002
AfaI GTAC 4 cut(s) 1008, 1061, 1203, 1283
AgeI ACCGGT 1 cut(s) 1439
AgsI TTSAA 3 cut(s) 653, 884, 1120
AjnI CCWGG 2 cut(s) 147, 708
AleI CACNNNNGTG 1 cut(s) 506
AloI GAACNNNNNNTCC 6 cut(s) 94, 126, 840, 857, 872, 889
AluBI AGCT 5 cut(s) 256, 815, 1262, 1339, 1570
AluI AGCT 5 cut(s) 256, 815, 1262, 1339, 1570
Alw26I GTCTC 3 cut(s) 206, 536, 1037
AlwI GGATC 2 cut(s) 117, 1409
Ama87I CYCGRG 3 cut(s) 53, 936, 1320
AoxI GGCC 5 cut(s) 42, 420, 711, 1224, 1434
ApeKI GCWGC 4 cut(s) 890, 1208, 1262, 1445
ApoI RAATTY 5 cut(s) 115, 400, 588, 719, 841
AsiGI ACCGGT 1 cut(s) 1439
Asp700I GAANNNNTTC 3 cut(s) 702, 872, 1115
AspS9I GGNCC 3 cut(s) 110, 284, 712
AsuHPI GGTGA 8 cut(s) 197, 446, 485, 520, 527, 943, 1231, 1567
AsuII TTCGAA 1 cut(s) 728
AvaI CYCGRG 3 cut(s) 53, 936, 1320
AvaII GGWCC 2 cut(s) 110, 284
BaeGI GKGCMC 1 cut(s) 320
BauI CACGAG 1 cut(s) 975
BbsI GAAGAC 1 cut(s) 1108
BbvI GCAGC 4 cut(s) 877, 1220, 1249, 1457
BccI CCATC 6 cut(s) 56, 245, 812, 1007, 1082, 1338
BceAI ACGGC 1 cut(s) 407
BcgI CGANNNNNNTGC 1 cut(s) 32
BciT130I CCWGG 2 cut(s) 149, 710
BclI TGATCA 2 cut(s) 361, 598
BcoDI GTCTC 3 cut(s) 206, 536, 1037
BfaI CTAG 3 cut(s) 272, 620, 1034
BfuAI ACCTGC 1 cut(s) 286
BisI GCNGC 4 cut(s) 891, 1209, 1263, 1446
BlsI GCNGC 4 cut(s) 892, 1210, 1264, 1447
BmcAI AGTACT 1 cut(s) 1008
Bme1390I CCNGG 2 cut(s) 149, 710
Bme18I GGWCC 2 cut(s) 110, 284
BmeT110I CYCGRG 3 cut(s) 53, 936, 1320
BmgT120I GGNCC 3 cut(s) 110, 284, 712
BmiI GGNNCC 1 cut(s) 285
BmrFI CCNGG 2 cut(s) 149, 710
BmsI GCATC 4 cut(s) 364, 1499, 1534, 1556
BpiI GAAGAC 1 cut(s) 1108
BplI GAGNNNNNCTC 2 cut(s) 122, 154
BpmI CTGGAG 2 cut(s) 170, 515
Bpu14I TTCGAA 1 cut(s) 728
BpuEI CTTGAG 1 cut(s) 350
BsaAI YACGTR 1 cut(s) 1205
BsaBI GATNNNNATC 1 cut(s) 236
BsaI GGTCTC 1 cut(s) 1037
BsaJI CCNNGG 3 cut(s) 1101, 1319, 1431
BsaWI WCCGGW 3 cut(s) 140, 862, 1439
BsaXI ACNNNNNCTCC 2 cut(s) 857, 887
Bse118I RCCGGY 1 cut(s) 1439
Bse1I ACTGG 1 cut(s) 1014
Bse8I GATNNNNATC 1 cut(s) 236
BseBI CCWGG 2 cut(s) 149, 710
BseDI CCNNGG 3 cut(s) 1101, 1319, 1431
BseGI GGATG 5 cut(s) 48, 526, 1018, 1314, 1423
BseJI GATNNNNATC 1 cut(s) 236
BseMII CTCAG 6 cut(s) 218, 326, 495, 764, 1098, 1242
BseNI ACTGG 1 cut(s) 1014
BseRI GAGGAG 1 cut(s) 11
BseSI GKGCMC 1 cut(s) 320
BseXI GCAGC 4 cut(s) 877, 1220, 1249, 1457
BsgI GTGCAG 5 cut(s) 282, 446, 1059, 1227, 1464
Bsh1236I CGCG 1 cut(s) 837
BshFI GGCC 5 cut(s) 44, 422, 713, 1226, 1436
BshTI ACCGGT 1 cut(s) 1439
BsiHKCI CYCGRG 3 cut(s) 53, 936, 1320
BsiSI CCGG 3 cut(s) 141, 863, 1440
BsmAI GTCTC 3 cut(s) 206, 536, 1037
BsnI GGCC 5 cut(s) 44, 422, 713, 1226, 1436
Bso31I GGTCTC 1 cut(s) 1037
BsoBI CYCGRG 3 cut(s) 53, 936, 1320
Bsp119I TTCGAA 1 cut(s) 728
Bsp1286I GDGCHC 1 cut(s) 320
Bsp143I GATC 7 cut(s) 122, 361, 598, 616, 1272, 1414, 1513
BspACI CCGC 4 cut(s) 837, 1051, 1199, 1428
BspANI GGCC 5 cut(s) 44, 422, 713, 1226, 1436
BspCNI CTCAG 6 cut(s) 219, 325, 496, 763, 1099, 1241
BspFNI CGCG 1 cut(s) 837
BspHI TCATGA 1 cut(s) 1510
BspLI GGNNCC 1 cut(s) 285
BspMI ACCTGC 1 cut(s) 286
BspPI GGATC 2 cut(s) 117, 1409
BspQI GCTCTTC 1 cut(s) 1252
BspT104I TTCGAA 1 cut(s) 728
BspTNI GGTCTC 1 cut(s) 1037
BsrFI RCCGGY 1 cut(s) 1439
BsrI ACTGG 1 cut(s) 1014
BssAI RCCGGY 1 cut(s) 1439
BssECI CCNNGG 3 cut(s) 1101, 1319, 1431
BssMI GATC 7 cut(s) 122, 361, 598, 616, 1272, 1414, 1513
BssNAI GTATAC 1 cut(s) 570
BssSI CACGAG 1 cut(s) 975
Bst1107I GTATAC 1 cut(s) 570
Bst2BI CACGAG 1 cut(s) 975
Bst2UI CCWGG 2 cut(s) 149, 710
Bst4CI ACNGT 3 cut(s) 174, 280, 950
Bst6I CTCTTC 2 cut(s) 1252, 1569
BstBAI YACGTR 1 cut(s) 1205
BstBI TTCGAA 1 cut(s) 728
BstC8I GCNNGC 4 cut(s) 414, 835, 924, 1410
BstDEI CTNAG 8 cut(s) 63, 227, 312, 504, 750, 965, 1107, 1228
BstF5I GGATG 5 cut(s) 48, 526, 1018, 1314, 1423
BstFNI CGCG 1 cut(s) 837
BstKTI GATC 7 cut(s) 125, 364, 601, 619, 1275, 1417, 1516
BstMAI GTCTC 3 cut(s) 206, 536, 1037
BstMBI GATC 7 cut(s) 122, 361, 598, 616, 1272, 1414, 1513
BstMWI GCNNNNNNNGC 6 cut(s) 64, 554, 1196, 1205, 1367, 1442
BstNI CCWGG 2 cut(s) 149, 710
BstNSI RCATGY 1 cut(s) 581
BstSCI CCNGG 2 cut(s) 147, 708
BstSLI GKGCMC 1 cut(s) 320
BstUI CGCG 1 cut(s) 837
BstV1I GCAGC 4 cut(s) 877, 1220, 1249, 1457
BstV2I GAAGAC 1 cut(s) 1108
BstX2I RGATCY 1 cut(s) 1414
BstYI RGATCY 1 cut(s) 1414
BstZ17I GTATAC 1 cut(s) 570
BsuRI GGCC 5 cut(s) 44, 422, 713, 1226, 1436
BtsCI GGATG 5 cut(s) 48, 526, 1018, 1314, 1423
BtsI GCAGTG 1 cut(s) 933
BtsIMutI CAGTG 5 cut(s) 285, 320, 501, 933, 1236
BveI ACCTGC 1 cut(s) 286
Cac8I GCNNGC 4 cut(s) 414, 835, 924, 1410
CciI TCATGA 1 cut(s) 1510
Cfr10I RCCGGY 1 cut(s) 1439
Cfr13I GGNCC 3 cut(s) 110, 284, 712
CseI GACGC 1 cut(s) 1031
Csp6I GTAC 4 cut(s) 1007, 1060, 1202, 1282
CspAI ACCGGT 1 cut(s) 1439
CviAII CATG 7 cut(s) 186, 578, 692, 739, 910, 1177, 1511
CviQI GTAC 4 cut(s) 1007, 1060, 1202, 1282
DdeI CTNAG 8 cut(s) 63, 227, 312, 504, 750, 965, 1107, 1228
DpnI GATC 7 cut(s) 124, 363, 600, 618, 1274, 1416, 1515
DpnII GATC 7 cut(s) 122, 361, 598, 616, 1272, 1414, 1513
Eam1104I CTCTTC 2 cut(s) 1252, 1569
EarI CTCTTC 2 cut(s) 1252, 1569
Eco147I AGGCCT 1 cut(s) 1226
Eco31I GGTCTC 1 cut(s) 1037
Eco32I GATATC 1 cut(s) 802
Eco47I GGWCC 2 cut(s) 110, 284
Eco57I CTGAAG 2 cut(s) 717, 1002
Eco88I CYCGRG 3 cut(s) 53, 936, 1320
EcoRII CCWGG 2 cut(s) 147, 708
EcoRV GATATC 1 cut(s) 802
EcoT22I ATGCAT 1 cut(s) 1549
FaeI CATG 7 cut(s) 189, 581, 695, 742, 913, 1180, 1514
FatI CATG 7 cut(s) 185, 577, 691, 738, 909, 1176, 1510
FauI CCCGC 2 cut(s) 1044, 1435
FbaI TGATCA 2 cut(s) 361, 598
FblI GTMKAC 2 cut(s) 569, 945
Fnu4HI GCNGC 4 cut(s) 891, 1209, 1263, 1446
FokI GGATG 5 cut(s) 35, 533, 1025, 1301, 1410
Fsp4HI GCNGC 4 cut(s) 891, 1209, 1263, 1446
FspBI CTAG 3 cut(s) 272, 620, 1034
GluI GCNGC 4 cut(s) 891, 1209, 1263, 1446
GsuI CTGGAG 2 cut(s) 170, 515
HaeIII GGCC 5 cut(s) 44, 422, 713, 1226, 1436
HapII CCGG 3 cut(s) 141, 863, 1440
HgaI GACGC 1 cut(s) 1031
Hin1II CATG 7 cut(s) 189, 581, 695, 742, 913, 1180, 1514
HinfI GANTC 4 cut(s) 10, 934, 1181, 1454
HpaII CCGG 3 cut(s) 141, 863, 1440
HphI GGTGA 8 cut(s) 197, 446, 485, 520, 527, 943, 1231, 1567
Hpy166II GTNNAC 3 cut(s) 570, 898, 946
Hpy188I TCNGA 6 cut(s) 9, 38, 228, 753, 1343, 1391
Hpy188III TCNNGA 8 cut(s) 120, 241, 287, 329, 365, 620, 1254, 1511
Hpy8I GTNNAC 3 cut(s) 570, 898, 946
HpyAV CCTTC 2 cut(s) 69, 1216
HpyCH4III ACNGT 3 cut(s) 174, 280, 950
HpyCH4IV ACGT 3 cut(s) 342, 641, 1204
HpyF10VI GCNNNNNNNGC 6 cut(s) 64, 554, 1196, 1205, 1367, 1442
HpyF3I CTNAG 8 cut(s) 63, 227, 312, 504, 750, 965, 1107, 1228
HpySE526I ACGT 3 cut(s) 342, 641, 1204
Hsp92II CATG 7 cut(s) 189, 581, 695, 742, 913, 1180, 1514
Ksp22I TGATCA 2 cut(s) 361, 598
Kzo9I GATC 7 cut(s) 122, 361, 598, 616, 1272, 1414, 1513
LguI GCTCTTC 1 cut(s) 1252
LmnI GCTCC 2 cut(s) 24, 1344
Lsp1109I GCAGC 4 cut(s) 877, 1220, 1249, 1457
LweI GCATC 4 cut(s) 364, 1499, 1534, 1556
MaeI CTAG 3 cut(s) 272, 620, 1034
MaeII ACGT 3 cut(s) 342, 641, 1204
MaeIII GTNAC 4 cut(s) 434, 499, 931, 1019
MalI GATC 7 cut(s) 124, 363, 600, 618, 1274, 1416, 1515
MboI GATC 7 cut(s) 122, 361, 598, 616, 1272, 1414, 1513
MboII GAAGA 5 cut(s) 710, 1022, 1108, 1269, 1570
MflI RGATCY 1 cut(s) 1414
MhlI GDGCHC 1 cut(s) 320
MluCI AATT 8 cut(s) 115, 400, 588, 719, 822, 841, 1026, 1458
MlyI GAGTC 2 cut(s) 928, 1463
MmeI TCCRAC 2 cut(s) 79, 1066
Mph1103I ATGCAT 1 cut(s) 1549
MroXI GAANNNNTTC 3 cut(s) 702, 872, 1115
MseI TTAA 4 cut(s) 785, 1137, 1215, 1326
MslI CAYNNNNRTG 4 cut(s) 506, 795, 1217, 1515
MspI CCGG 3 cut(s) 141, 863, 1440
MspR9I CCNGG 2 cut(s) 149, 710
MvaI CCWGG 2 cut(s) 149, 710
MvnI CGCG 1 cut(s) 837
MwoI GCNNNNNNNGC 6 cut(s) 64, 554, 1196, 1205, 1367, 1442
NdeII GATC 7 cut(s) 122, 361, 598, 616, 1272, 1414, 1513
NlaIII CATG 7 cut(s) 189, 581, 695, 742, 913, 1180, 1514
NlaIV GGNNCC 1 cut(s) 285
NmeAIII GCCGAG 2 cut(s) 1126, 1456
NmuCI GTSAC 4 cut(s) 434, 499, 931, 1019
NsiI ATGCAT 1 cut(s) 1549
NspI RCATGY 1 cut(s) 581
NspV TTCGAA 1 cut(s) 728
OliI CACNNNNGTG 1 cut(s) 506
PaeR7I CTCGAG 2 cut(s) 53, 936
PagI TCATGA 1 cut(s) 1510
PceI AGGCCT 1 cut(s) 1226
PciSI GCTCTTC 1 cut(s) 1252
PcsI WCGNNNNNNNCGW 1 cut(s) 712
PdmI GAANNNNTTC 3 cut(s) 702, 872, 1115
PfeI GAWTC 2 cut(s) 10, 1181
PinAI ACCGGT 1 cut(s) 1439
PkrI GCNGC 4 cut(s) 892, 1210, 1264, 1447
PleI GAGTC 2 cut(s) 928, 1462
PpsI GAGTC 2 cut(s) 928, 1462
Ppu21I YACGTR 1 cut(s) 1205
PsiI TTATAA 1 cut(s) 1502
Psp6I CCWGG 2 cut(s) 147, 708
PspGI CCWGG 2 cut(s) 147, 708
PspN4I GGNNCC 1 cut(s) 285
PspPI GGNCC 3 cut(s) 110, 284, 712
PspXI VCTCGAGB 2 cut(s) 53, 936
PsuI RGATCY 1 cut(s) 1414
RsaI GTAC 4 cut(s) 1008, 1061, 1203, 1283
RsaNI GTAC 4 cut(s) 1007, 1060, 1202, 1282
RseI CAYNNNNRTG 4 cut(s) 506, 795, 1217, 1515
SapI GCTCTTC 1 cut(s) 1252
SaqAI TTAA 4 cut(s) 785, 1137, 1215, 1326
SatI GCNGC 4 cut(s) 891, 1209, 1263, 1446
Sau3AI GATC 7 cut(s) 122, 361, 598, 616, 1272, 1414, 1513
Sau96I GGNCC 3 cut(s) 110, 284, 712
ScaI AGTACT 1 cut(s) 1008
SchI GAGTC 2 cut(s) 928, 1463
ScrFI CCNGG 2 cut(s) 149, 710
SduI GDGCHC 1 cut(s) 320
SfaNI GCATC 4 cut(s) 364, 1499, 1534, 1556
Sfr274I CTCGAG 2 cut(s) 53, 936
SfuI TTCGAA 1 cut(s) 728
SgrAI CRCCGGYG 1 cut(s) 1439
SinI GGWCC 2 cut(s) 110, 284
SlaI CTCGAG 2 cut(s) 53, 936
SmiMI CAYNNNNRTG 4 cut(s) 506, 795, 1217, 1515
SmlI CTYRAG 4 cut(s) 53, 329, 936, 1565
SmoI CTYRAG 4 cut(s) 53, 329, 936, 1565
Sse9I AATT 8 cut(s) 115, 400, 588, 719, 822, 841, 1026, 1458
SseBI AGGCCT 1 cut(s) 1226
SsiI CCGC 4 cut(s) 837, 1051, 1199, 1428
SspI AATATT 1 cut(s) 661
SspMI CTAG 3 cut(s) 272, 620, 1034
StuI AGGCCT 1 cut(s) 1226
StyD4I CCNGG 2 cut(s) 147, 708
TaaI ACNGT 3 cut(s) 174, 280, 950
TaiI ACGT 3 cut(s) 345, 644, 1207
TaqI TCGA 7 cut(s) 54, 563, 728, 768, 937, 1068, 1420
TasI AATT 8 cut(s) 115, 400, 588, 719, 822, 841, 1026, 1458
TatI WGTACW 2 cut(s) 1006, 1281
TfiI GAWTC 2 cut(s) 10, 1181
Tru1I TTAA 4 cut(s) 785, 1137, 1215, 1326
Tru9I TTAA 4 cut(s) 785, 1137, 1215, 1326
TscAI CASTG 5 cut(s) 285, 320, 508, 933, 1236
TseFI GTSAC 4 cut(s) 434, 499, 931, 1019
TseI GCWGC 4 cut(s) 890, 1208, 1262, 1445
Tsp45I GTSAC 4 cut(s) 434, 499, 931, 1019
TspDTI ATGAA 2 cut(s) 755, 1239
TspGWI ACGGA 1 cut(s) 115
TspRI CASTG 5 cut(s) 285, 320, 508, 933, 1236
VpaK11BI GGWCC 2 cut(s) 110, 284
XapI RAATTY 5 cut(s) 115, 400, 588, 719, 841
XbaI TCTAGA 1 cut(s) 619
XceI RCATGY 1 cut(s) 581
XcmI CCANNNNNNNNNTGG 1 cut(s) 1006
XhoI CTCGAG 2 cut(s) 53, 936
XmiI GTMKAC 2 cut(s) 569, 945
XmnI GAANNNNTTC 3 cut(s) 702, 872, 1115
XspI CTAG 3 cut(s) 272, 620, 1034
ZrmI AGTACT 1 cut(s) 1008
Zsp2I ATGCAT 1 cut(s) 1549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.