MD01G1101400.v1.1

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002588
Physical Location & Seq
Reverse (-)
21436265 .. 21438061
1797 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1101400.v1.1.491

Sequence Viewer

Length: 1797 bp
ATGTTGGTTTCTGGACCTGGTGGAGGATGGGAGTGGAGTAACACCAAAACCCTAACCCTCCAAGTCCTCAATGGGCGGTGGTTCACGGTGTTTGCTTCCCTCTTAATCATGTCAGCCTCTGGCGCCACCTACATGTTTGGTCTTTACTCCAGTGACATCAAATCTGTGCTGGGATATGACCAAAGTACCCTCAACCTTCTCAGCTTGTTCAAGGACTTCGGGTCCAATGTTGGTGTCCTTTCTGGTCTGATTAACGAGGTCACATCCCCATGGGTGGTCCTATCAGTGGGTGGAGTCCTCAATTTTTTTGGTTACTTCATGATTTGGATGGCCGTGACCAAAAAAATTGCTAAGCCTCAAGTTTGGCACATGTGCATGTACATTTGTATGGGTGCAACTTCTCAGTCTTTTGCCAATACTGGATCACTTATCACTAGTGTCAAGAACTTCCCGGAGAGCCGCGGCTCCGTTTTGGGGTTCTTGAAGGGATATGCTGGCCTAAGTGGTGCTATATTCACACAGTTATACTCTGCTTTTTATTATGACGACACCAAGGATTTGATTTTGTTGATTGGGTGGCTTCCTACTGCCGTTTCTTTTGTTTCTCTTAGAACAATTCGGATTATGAAGGTCATTAGGCACCCCAATGAGCTCAAAGTGTTTTATAATATGCTTTATATCTCACTTGGGCTTGCTGGATTTTTAATGGTTATGATTATAATCCAGAAGAATGTTCATTTCACTCAAGGTGAGTACGGTGCTAGTGCTGCCATGGTTATTTTCTTGCTTGTCCTCCCACTTGCTGTGGTTATTTTTGAGGAATTTAAGATCTTGGAAGGCAAGAAGCTCGAGTTGAATGGCGGTTCGGACTTGAAAGTAGTTACCGAAAAGCCAAAATCAGAAGAAGTTGGCGTTACATCTGATCTGTCACGGCCCAAGGAAGCGCTTGCCACCACTACCAGAGAGACTGAACCGTCATGCTGGAAAACTATGTTTAGGCCACCAGAGAGAGGTGAGGACTACACCATACTCCAAGCACTTTTCAGCATTGACATGCTCATTCTTTTCGTAGCATGCATTTGTGGTGTTGGAGGCGCATTTACAGCAATAGACAACTTGGGTCAGATTGGAGCTTCTTTAGGGTACCATAAACACAGCATAAGCACTTTCGTGTCACTAGTAAGCATATGGAATTATTTGGGTCGGGTGGTGTCGGGTTTCGGCTCCGAAATCTTGTTAAAAAGATACAAATTCCCTCGTCCTCTAATGCTAACCCTAACCCTCTTGCTCTCTTGTGCTGGTCACCTTCTCATTGCCTTCAATGTCAAAAATGGTCTCTACGTAGCCTCCGTGATAATAGGGTTTTGTTTAGGTGCCTTATGGCCTCTACTTTTCGCCATAATTTCTGAGCTTTTCGGCCTCAAATACTACTCGACTTTGTTCAATTTTGGGGCATGCGCTAGCCCGGTTGGACTATACTTGCTCAATGTGAGACTCGCCGGGCACATATATGACAAAGAAGCCAATAAGCAGCTTGCAGCAAAAGGGCTTGTGAGAGAAGTAGGTGAGGAGCTGACTTGCGTTGGGGGAGAGTGCTTCAAATTGTCCTTCATCGTGATTGCAGCAGTTACATTATTTGGCACATTTGTGTCGCTGGTTTTGGTGATGAGAACGAGAAAGTTTTACCAGAGTGACATATACAAGAAGTTCAGAGAGAAAACAGAAGCTGCTGAGGGAGAGATGGCCATGGCTGGTGAAAATGGTGTCAAACCTTTGGCAACCAATGGAAGATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 Nodulin-like

Protein Analysis

599

Amino Acids

65.7

Weight (kDa)

8.93

Isoelectric Point (pI)

33.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 21 - 172 6.9e-06 Major Facilitator Superfamily
Nodulin-like PF06813 26 - 271 6.5e-89 Nodulin-like
MFS_1 PF07690 322 - 485 1.5e-06 Major Facilitator Superfamily
NFD4_C PF23262 347 - 562 1.1e-17 NFD4, C-terminal domain
MFS_1 PF07690 374 - 559 1.2e-07 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 666, 719
AasI GACNNNNNNGTC 1 cut(s) 973
Acc65I GGTACC 1 cut(s) 1143
AccB1I GGYRCC 4 cut(s) 122, 639, 1143, 1373
AccII CGCG 1 cut(s) 462
AciI CCGC 4 cut(s) 76, 460, 462, 861
AclWI GGATC 1 cut(s) 430
AcoI YGGCCR 2 cut(s) 330, 1743
AcsI RAATTY 2 cut(s) 821, 1250
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 4 cut(s) 187, 380, 755, 1145
AfeI AGCGCT 1 cut(s) 945
AfiI CCNNNNNNNGG 5 cut(s) 23, 274, 286, 474, 1010
AflIII ACRYGT 2 cut(s) 132, 369
AgsI TTSAA 7 cut(s) 211, 484, 856, 874, 1321, 1444, 1600
AhdI GACNNNNNGTC 1 cut(s) 220
AhlI ACTAGT 2 cut(s) 434, 1177
AjnI CCWGG 1 cut(s) 16
AleI CACNNNNGTG 2 cut(s) 1169, 1646
AluBI AGCT 8 cut(s) 204, 652, 847, 1133, 1411, 1534, 1573, 1727
AluI AGCT 8 cut(s) 204, 652, 847, 1133, 1411, 1534, 1573, 1727
Alw21I GWGCWC 1 cut(s) 654
Alw26I GTCTC 3 cut(s) 959, 1340, 1486
AlwI GGATC 1 cut(s) 430
AlwNI CAGNNNCTG 2 cut(s) 119, 1727
Ama87I CYCGRG 1 cut(s) 848
Aor51HI AGCGCT 1 cut(s) 945
AoxI GGCC 7 cut(s) 330, 496, 932, 998, 1382, 1417, 1743
ApeKI GCWGC 5 cut(s) 767, 1531, 1538, 1622, 1727
ApoI RAATTY 2 cut(s) 821, 1250
Asp718I GGTACC 1 cut(s) 1143
AspLEI GCGC 4 cut(s) 125, 946, 1097, 1460
AspS9I GGNCC 4 cut(s) 14, 222, 277, 933
AsuC2I CCSGG 3 cut(s) 452, 1466, 1501
AsuHPI GGTGA 6 cut(s) 761, 1025, 1295, 1577, 1675, 1766
AsuNHI GCTAGC 1 cut(s) 1460
AvaI CYCGRG 1 cut(s) 848
AvaII GGWCC 3 cut(s) 14, 222, 277
BaeGI GKGCMC 1 cut(s) 1506
BaeI ACNNNNGTAYC 2 cut(s) 169, 202
BalI TGGCCA 1 cut(s) 1745
BanI GGYRCC 4 cut(s) 122, 639, 1143, 1373
BanII GRGCYC 1 cut(s) 654
Bbv12I GWGCWC 1 cut(s) 654
BbvCI CCTCAGC 1 cut(s) 1731
BbvI GCAGC 5 cut(s) 754, 1543, 1550, 1634, 1714
BccI CCATC 3 cut(s) 21, 322, 1735
BceAI ACGGC 3 cut(s) 317, 575, 947
BcgI CGANNNNNNTGC 2 cut(s) 829, 863
BciT130I CCWGG 1 cut(s) 18
BcnI CCSGG 3 cut(s) 452, 1466, 1501
BcoDI GTCTC 3 cut(s) 959, 1340, 1486
BcuI ACTAGT 2 cut(s) 434, 1177
BfaI CTAG 4 cut(s) 435, 762, 1178, 1461
BfoI RGCGCY 2 cut(s) 126, 947
BglII AGATCT 1 cut(s) 828
BisI GCNGC 7 cut(s) 460, 463, 768, 1532, 1539, 1623, 1728
BlpI GCTNAGC 1 cut(s) 351
BlsI GCNGC 7 cut(s) 461, 464, 769, 1533, 1540, 1624, 1729
Bme1390I CCNGG 4 cut(s) 18, 452, 1466, 1501
Bme18I GGWCC 3 cut(s) 14, 222, 277
BmeRI GACNNNNNGTC 1 cut(s) 220
BmeT110I CYCGRG 1 cut(s) 848
BmgT120I GGNCC 4 cut(s) 14, 222, 277, 933
BmiI GGNNCC 7 cut(s) 124, 223, 466, 641, 1145, 1225, 1375
BmrFI CCNGG 4 cut(s) 18, 452, 1466, 1501
BmtI GCTAGC 1 cut(s) 1464
BpmI CTGGAG 1 cut(s) 133
Bpu10I CCTNAGC 1 cut(s) 1731
Bpu1102I GCTNAGC 1 cut(s) 351
BpuEI CTTGAG 2 cut(s) 342, 729
BpuMI CCSGG 3 cut(s) 452, 1466, 1501
BsaAI YACGTR 1 cut(s) 1342
BsaBI GATNNNNATC 1 cut(s) 719
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 1340
BsaJI CCNNGG 6 cut(s) 269, 460, 552, 771, 936, 1746
BsaXI ACNNNNNCTCC 2 cut(s) 1331, 1361
Bsc4I CCNNNNNNNGG 5 cut(s) 23, 274, 286, 474, 1010
Bse1I ACTGG 2 cut(s) 150, 424
Bse3DI GCAATG 1 cut(s) 1311
Bse8I GATNNNNATC 1 cut(s) 719
BseBI CCWGG 1 cut(s) 18
BseDI CCNNGG 6 cut(s) 269, 460, 552, 771, 936, 1746
BseGI GGATG 3 cut(s) 32, 263, 333
BseJI GATNNNNATC 1 cut(s) 719
BseLI CCNNNNNNNGG 5 cut(s) 23, 274, 286, 474, 1010
BseMI GCAATG 1 cut(s) 1311
BseMII CTCAG 4 cut(s) 214, 416, 1398, 1722
BseNI ACTGG 2 cut(s) 150, 424
BseRI GAGGAG 1 cut(s) 1583
BseSI GKGCMC 1 cut(s) 1506
BseXI GCAGC 5 cut(s) 754, 1543, 1550, 1634, 1714
BseYI CCCAGC 1 cut(s) 169
Bsh1236I CGCG 1 cut(s) 462
BshFI GGCC 7 cut(s) 332, 498, 934, 1000, 1384, 1419, 1745
BshNI GGYRCC 4 cut(s) 122, 639, 1143, 1373
BsiHKAI GWGCWC 1 cut(s) 654
BsiHKCI CYCGRG 1 cut(s) 848
BsiSI CCGG 3 cut(s) 452, 1466, 1500
BslI CCNNNNNNNGG 5 cut(s) 23, 274, 286, 474, 1010
BsmAI GTCTC 3 cut(s) 959, 1340, 1486
BsnI GGCC 7 cut(s) 332, 498, 934, 1000, 1384, 1419, 1745
Bso31I GGTCTC 1 cut(s) 1340
BsoBI CYCGRG 1 cut(s) 848
Bsp1286I GDGCHC 2 cut(s) 654, 1506
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 4 cut(s) 422, 828, 922, 1790
Bsp1720I GCTNAGC 1 cut(s) 351
Bsp19I CCATGG 3 cut(s) 269, 771, 1746
BspACI CCGC 4 cut(s) 76, 460, 462, 861
BspANI GGCC 7 cut(s) 332, 498, 934, 1000, 1384, 1419, 1745
BspCNI CTCAG 4 cut(s) 213, 415, 1399, 1723
BspFNI CGCG 1 cut(s) 462
BspHI TCATGA 1 cut(s) 318
BspLI GGNNCC 7 cut(s) 124, 223, 466, 641, 1145, 1225, 1375
BspOI GCTAGC 1 cut(s) 1464
BspPI GGATC 1 cut(s) 430
BspT107I GGYRCC 4 cut(s) 122, 639, 1143, 1373
BspTNI GGTCTC 1 cut(s) 1340
BsrDI GCAATG 1 cut(s) 1311
BsrGI TGTACA 1 cut(s) 378
BsrI ACTGG 2 cut(s) 150, 424
BssECI CCNNGG 6 cut(s) 269, 460, 552, 771, 936, 1746
BssMI GATC 4 cut(s) 422, 828, 922, 1790
BssNI GRCGYC 1 cut(s) 123
BssT1I CCWWGG 5 cut(s) 269, 552, 771, 936, 1746
Bst2UI CCWGG 1 cut(s) 18
Bst4CI ACNGT 4 cut(s) 88, 522, 758, 975
BstACI GRCGYC 1 cut(s) 123
BstAUI TGTACA 1 cut(s) 378
BstBAI YACGTR 1 cut(s) 1342
BstC8I GCNNGC 7 cut(s) 496, 693, 948, 1075, 1456, 1462, 1536
BstDEI CTNAG 7 cut(s) 200, 351, 402, 500, 608, 1407, 1731
BstDSI CCRYGG 4 cut(s) 269, 460, 771, 1746
BstEII GGTNACC 1 cut(s) 1301
BstENI CCTNNNNNAGG 1 cut(s) 21
BstF5I GGATG 3 cut(s) 32, 263, 333
BstFNI CGCG 1 cut(s) 462
BstH2I RGCGCY 2 cut(s) 126, 947
BstHHI GCGC 4 cut(s) 125, 946, 1097, 1460
BstKTI GATC 4 cut(s) 425, 831, 925, 1793
BstMAI GTCTC 3 cut(s) 959, 1340, 1486
BstMBI GATC 4 cut(s) 422, 828, 922, 1790
BstMWI GCNNNNNNNGC 3 cut(s) 122, 767, 1103
BstNI CCWGG 1 cut(s) 18
BstNSI RCATGY 6 cut(s) 136, 373, 379, 1057, 1077, 1458
BstPI GGTNACC 1 cut(s) 1301
BstSCI CCNGG 4 cut(s) 16, 450, 1464, 1499
BstSLI GKGCMC 1 cut(s) 1506
BstSNI TACGTA 1 cut(s) 1342
BstUI CGCG 1 cut(s) 462
BstV1I GCAGC 5 cut(s) 754, 1543, 1550, 1634, 1714
BstX2I RGATCY 1 cut(s) 828
BstYI RGATCY 1 cut(s) 828
BsuRI GGCC 7 cut(s) 332, 498, 934, 1000, 1384, 1419, 1745
BtgI CCRYGG 4 cut(s) 269, 460, 771, 1746
BtsCI GGATG 3 cut(s) 32, 263, 333
BtsIMutI CAGTG 2 cut(s) 157, 291
Cac8I GCNNGC 7 cut(s) 496, 693, 948, 1075, 1456, 1462, 1536
CaiI CAGNNNCTG 2 cut(s) 119, 1727
CciI TCATGA 1 cut(s) 318
CfoI GCGC 4 cut(s) 125, 946, 1097, 1460
Cfr13I GGNCC 4 cut(s) 14, 222, 277, 933
Cfr42I CCGCGG 1 cut(s) 463
CsiI ACCWGGT 1 cut(s) 16
Csp6I GTAC 4 cut(s) 186, 379, 754, 1144
CviQI GTAC 4 cut(s) 186, 379, 754, 1144
DdeI CTNAG 7 cut(s) 200, 351, 402, 500, 608, 1407, 1731
DinI GGCGCC 1 cut(s) 124
DpnI GATC 4 cut(s) 424, 830, 924, 1792
DpnII GATC 4 cut(s) 422, 828, 922, 1790
DrdI GACNNNNNNGTC 1 cut(s) 973
DriI GACNNNNNGTC 1 cut(s) 220
DseDI GACNNNNNNGTC 1 cut(s) 973
EaeI YGGCCR 2 cut(s) 330, 1743
Eam1105I GACNNNNNGTC 1 cut(s) 220
Ecl136II GAGCTC 1 cut(s) 652
Eco105I TACGTA 1 cut(s) 1342
Eco130I CCWWGG 5 cut(s) 269, 552, 771, 936, 1746
Eco24I GRGCYC 1 cut(s) 654
Eco31I GGTCTC 1 cut(s) 1340
Eco47I GGWCC 3 cut(s) 14, 222, 277
Eco47III AGCGCT 1 cut(s) 945
Eco53kI GAGCTC 1 cut(s) 652
Eco88I CYCGRG 1 cut(s) 848
Eco91I GGTNACC 1 cut(s) 1301
EcoICRI GAGCTC 1 cut(s) 652
EcoNI CCTNNNNNAGG 1 cut(s) 21
EcoO65I GGTNACC 1 cut(s) 1301
EcoRII CCWGG 1 cut(s) 16
EcoT14I CCWWGG 5 cut(s) 269, 552, 771, 936, 1746
EcoT22I ATGCAT 1 cut(s) 1079
EcoT38I GRGCYC 1 cut(s) 654
EgeI GGCGCC 1 cut(s) 124
EheI GGCGCC 1 cut(s) 124
ErhI CCWWGG 5 cut(s) 269, 552, 771, 936, 1746
FauNDI CATATG 1 cut(s) 1187
Fnu4HI GCNGC 7 cut(s) 460, 463, 768, 1532, 1539, 1623, 1728
FokI GGATG 3 cut(s) 39, 250, 340
FriOI GRGCYC 1 cut(s) 654
Fsp4HI GCNGC 7 cut(s) 460, 463, 768, 1532, 1539, 1623, 1728
FspBI CTAG 4 cut(s) 435, 762, 1178, 1461
GlaI GCGC 4 cut(s) 124, 945, 1096, 1459
GluI GCNGC 7 cut(s) 460, 463, 768, 1532, 1539, 1623, 1728
GsaI CCCAGC 1 cut(s) 173
GsuI CTGGAG 1 cut(s) 133
HaeII RGCGCY 2 cut(s) 126, 947
HaeIII GGCC 7 cut(s) 332, 498, 934, 1000, 1384, 1419, 1745
HapII CCGG 3 cut(s) 452, 1466, 1500
HhaI GCGC 4 cut(s) 125, 946, 1097, 1460
Hin1I GRCGYC 1 cut(s) 123
Hin6I GCGC 4 cut(s) 123, 944, 1095, 1458
HinP1I GCGC 4 cut(s) 123, 944, 1095, 1458
HinfI GANTC 2 cut(s) 294, 1494
HpaII CCGG 3 cut(s) 452, 1466, 1500
HphI GGTGA 6 cut(s) 761, 1025, 1295, 1577, 1675, 1766
Hpy166II GTNNAC 1 cut(s) 84
Hpy188I TCNGA 9 cut(s) 249, 621, 868, 901, 922, 1125, 1228, 1408, 1712
Hpy188III TCNNGA 6 cut(s) 12, 319, 442, 481, 724, 1615
Hpy8I GTNNAC 1 cut(s) 84
HpyAV CCTTC 7 cut(s) 206, 478, 622, 830, 1316, 1327, 1618
HpyCH4III ACNGT 4 cut(s) 88, 522, 758, 975
HpyCH4IV ACGT 1 cut(s) 1341
HpyCH4V TGCA 5 cut(s) 375, 395, 1077, 1538, 1622
HpyF10VI GCNNNNNNNGC 3 cut(s) 122, 767, 1103
HpyF3I CTNAG 7 cut(s) 200, 351, 402, 500, 608, 1407, 1731
HpySE526I ACGT 1 cut(s) 1341
Hsp92I GRCGYC 1 cut(s) 123
HspAI GCGC 4 cut(s) 123, 944, 1095, 1458
KasI GGCGCC 1 cut(s) 122
KpnI GGTACC 1 cut(s) 1147
KspI CCGCGG 1 cut(s) 463
Kzo9I GATC 4 cut(s) 422, 828, 922, 1790
LmnI GCTCC 4 cut(s) 470, 1130, 1229, 1570
Lsp1109I GCAGC 5 cut(s) 754, 1543, 1550, 1634, 1714
MabI ACCWGGT 1 cut(s) 16
MaeI CTAG 4 cut(s) 435, 762, 1178, 1461
MaeII ACGT 1 cut(s) 1341
MalI GATC 4 cut(s) 424, 830, 924, 1792
MboI GATC 4 cut(s) 422, 828, 922, 1790
MboII GAAGA 2 cut(s) 739, 914
MflI RGATCY 1 cut(s) 828
MhlI GDGCHC 2 cut(s) 654, 1506
MlsI TGGCCA 1 cut(s) 1745
MluCI AATT 9 cut(s) 301, 345, 615, 821, 1192, 1250, 1401, 1444, 1601
MluNI TGGCCA 1 cut(s) 1745
Mly113I GGCGCC 1 cut(s) 123
MlyI GAGTC 2 cut(s) 303, 1488
MmeI TCCRAC 2 cut(s) 1069, 1450
Mox20I TGGCCA 1 cut(s) 1745
Mph1103I ATGCAT 1 cut(s) 1079
MscI TGGCCA 1 cut(s) 1745
MseI TTAA 5 cut(s) 104, 252, 704, 825, 1238
MslI CAYNNNNRTG 9 cut(s) 131, 268, 374, 386, 645, 1052, 1169, 1509, 1646
Msp20I TGGCCA 1 cut(s) 1745
MspA1I CMGCKG 1 cut(s) 462
MspI CCGG 3 cut(s) 452, 1466, 1500
MspR9I CCNGG 4 cut(s) 18, 452, 1466, 1501
MvaI CCWGG 1 cut(s) 18
MvnI CGCG 1 cut(s) 462
MwoI GCNNNNNNNGC 3 cut(s) 122, 767, 1103
NarI GGCGCC 1 cut(s) 123
NciI CCSGG 3 cut(s) 452, 1466, 1501
NcoI CCATGG 3 cut(s) 269, 771, 1746
NdeI CATATG 1 cut(s) 1187
NdeII GATC 4 cut(s) 422, 828, 922, 1790
NheI GCTAGC 1 cut(s) 1460
NlaIV GGNNCC 7 cut(s) 124, 223, 466, 641, 1145, 1225, 1375
NmuCI GTSAC 7 cut(s) 152, 259, 334, 927, 1173, 1301, 1691
NsiI ATGCAT 1 cut(s) 1079
NspI RCATGY 6 cut(s) 136, 373, 379, 1057, 1077, 1458
OliI CACNNNNGTG 2 cut(s) 1169, 1646
PaeI GCATGC 2 cut(s) 1077, 1458
PaeR7I CTCGAG 1 cut(s) 848
PagI TCATGA 1 cut(s) 318
PciI ACATGT 2 cut(s) 132, 369
PcsI WCGNNNNNNNCGW 1 cut(s) 1347
PfoI TCCNGGA 1 cut(s) 450
PkrI GCNGC 7 cut(s) 461, 464, 769, 1533, 1540, 1624, 1729
PleI GAGTC 2 cut(s) 302, 1488
PluTI GGCGCC 1 cut(s) 126
PpsI GAGTC 2 cut(s) 302, 1488
Ppu21I YACGTR 1 cut(s) 1342
PscI ACATGT 2 cut(s) 132, 369
PsiI TTATAA 2 cut(s) 666, 719
Psp124BI GAGCTC 1 cut(s) 654
Psp6I CCWGG 1 cut(s) 16
PspEI GGTNACC 1 cut(s) 1301
PspFI CCCAGC 1 cut(s) 169
PspGI CCWGG 1 cut(s) 16
PspN4I GGNNCC 7 cut(s) 124, 223, 466, 641, 1145, 1225, 1375
PspPI GGNCC 4 cut(s) 14, 222, 277, 933
PspXI VCTCGAGB 1 cut(s) 848
PstNI CAGNNNCTG 2 cut(s) 119, 1727
PsuI RGATCY 1 cut(s) 828
RsaI GTAC 4 cut(s) 187, 380, 755, 1145
RsaNI GTAC 4 cut(s) 186, 379, 754, 1144
RseI CAYNNNNRTG 9 cut(s) 131, 268, 374, 386, 645, 1052, 1169, 1509, 1646
SacI GAGCTC 1 cut(s) 654
SacII CCGCGG 1 cut(s) 463
SaqAI TTAA 5 cut(s) 104, 252, 704, 825, 1238
SatI GCNGC 7 cut(s) 460, 463, 768, 1532, 1539, 1623, 1728
Sau3AI GATC 4 cut(s) 422, 828, 922, 1790
Sau96I GGNCC 4 cut(s) 14, 222, 277, 933
SchI GAGTC 2 cut(s) 303, 1488
ScrFI CCNGG 4 cut(s) 18, 452, 1466, 1501
SduI GDGCHC 2 cut(s) 654, 1506
SexAI ACCWGGT 1 cut(s) 16
SfoI GGCGCC 1 cut(s) 124
Sfr274I CTCGAG 1 cut(s) 848
Sfr303I CCGCGG 1 cut(s) 463
SgrBI CCGCGG 1 cut(s) 463
SinI GGWCC 3 cut(s) 14, 222, 277
SlaI CTCGAG 1 cut(s) 848
SmiMI CAYNNNNRTG 9 cut(s) 131, 268, 374, 386, 645, 1052, 1169, 1509, 1646
SmlI CTYRAG 3 cut(s) 357, 744, 848
SmoI CTYRAG 3 cut(s) 357, 744, 848
SnaBI TACGTA 1 cut(s) 1342
SpeI ACTAGT 2 cut(s) 434, 1177
SphI GCATGC 2 cut(s) 1077, 1458
Sse9I AATT 9 cut(s) 301, 345, 615, 821, 1192, 1250, 1401, 1444, 1601
SsiI CCGC 4 cut(s) 76, 460, 462, 861
SspDI GGCGCC 1 cut(s) 122
SspMI CTAG 4 cut(s) 435, 762, 1178, 1461
SstI GAGCTC 1 cut(s) 654
StyD4I CCNGG 4 cut(s) 16, 450, 1464, 1499
StyI CCWWGG 5 cut(s) 269, 552, 771, 936, 1746
TaaI ACNGT 4 cut(s) 88, 522, 758, 975
TaiI ACGT 1 cut(s) 1344
TaqI TCGA 2 cut(s) 849, 1433
TasI AATT 9 cut(s) 301, 345, 615, 821, 1192, 1250, 1401, 1444, 1601
TatI WGTACW 1 cut(s) 378
TauI GCSGC 2 cut(s) 462, 465
Tru1I TTAA 5 cut(s) 104, 252, 704, 825, 1238
Tru9I TTAA 5 cut(s) 104, 252, 704, 825, 1238
TscAI CASTG 2 cut(s) 157, 291
TseFI GTSAC 7 cut(s) 152, 259, 334, 927, 1173, 1301, 1691
TseI GCWGC 5 cut(s) 767, 1531, 1538, 1622, 1727
Tsp45I GTSAC 7 cut(s) 152, 259, 334, 927, 1173, 1301, 1691
TspDTI ATGAA 4 cut(s) 307, 641, 725, 1600
TspGWI ACGGA 2 cut(s) 457, 1339
TspRI CASTG 2 cut(s) 157, 291
VpaK11BI GGWCC 3 cut(s) 14, 222, 277
XagI CCTNNNNNAGG 1 cut(s) 21
XapI RAATTY 2 cut(s) 821, 1250
XceI RCATGY 6 cut(s) 136, 373, 379, 1057, 1077, 1458
XcmI CCANNNNNNNNNTGG 1 cut(s) 68
XhoI CTCGAG 1 cut(s) 848
XspI CTAG 4 cut(s) 435, 762, 1178, 1461
Zsp2I ATGCAT 1 cut(s) 1079
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.