MD04G1090400.v1.1

transmembrane transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002588
Physical Location & Seq
Reverse (-)
16050262 .. 16050825
564 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1090400.v1.1.491

Sequence Viewer

Length: 564 bp
ATGCTGATCTTGTTCCTTGCCACAAGTTGTGGTGTAGGCTGCACTTTAACCTCCATCGACAACTTGGGTCAGATTGGGAGCTCATTAAGCTACCCAACTGACAGCACAAGCACTTTCATATTTCTGGTGAGTATGTGGAATTACCTCGGAAGAGTCACTTCCGGCTTCTCCTCAGAAGTGCTTTTGAAAAAATACAATTTCCCTCGCCCCCTCATGCTCACTTTTGTGCTTCTCTTGTCATGCGTTGGCCATCTTCTCATAGCCTTTCGCGTCCCCAATTCTCTTTACTTTGCCTCGGTGATCATCGGGTTCTATTTTGGGGCACAATGGCCATTGATTTTCACCATCATATCGGAAATCTTTGGCCTCAAGTACTACGCCACTCTGATCAATGTTGGTGGTGCTGCTAGCCCTATTGGGGCTTACCTTGTCAATGTTAAAGTGGCTGGAAATTTATACGACGCGGAGGCCTTGAAGCAAATGAAGGCTTTGGGAAGGACTAGAAAACCATGGGAGGGCTTAAGTTGTGTGGGAGTGCGCAATGCTACAAATTGGCTTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 Nodulin-like

Protein Analysis

188

Amino Acids

20.45

Weight (kDa)

9.25

Isoelectric Point (pI)

30.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NFD4_C PF23262 10 - 165 7.4e-10 NFD4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 539
AccII CGCG 2 cut(s) 270, 464
AciI CCGC 1 cut(s) 464
AcoI YGGCCR 2 cut(s) 247, 329
AcsI RAATTY 1 cut(s) 451
AfaI GTAC 1 cut(s) 374
AfiI CCNNNNNNNGG 2 cut(s) 418, 515
AflII CTTAAG 1 cut(s) 520
AgsI TTSAA 2 cut(s) 187, 475
AleI CACNNNNGTG 1 cut(s) 224
AluBI AGCT 2 cut(s) 81, 90
AluI AGCT 2 cut(s) 81, 90
Alw21I GWGCWC 1 cut(s) 83
AoxI GGCC 4 cut(s) 247, 329, 364, 468
ApeKI GCWGC 2 cut(s) 39, 404
ApoI RAATTY 1 cut(s) 451
AspLEI GCGC 1 cut(s) 540
AsuHPI GGTGA 3 cut(s) 139, 310, 334
AsuNHI GCTAGC 1 cut(s) 407
BaeGI GKGCMC 1 cut(s) 325
BalI TGGCCA 2 cut(s) 249, 331
BanII GRGCYC 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 83
BbvI GCAGC 2 cut(s) 26, 391
BccI CCATC 3 cut(s) 62, 258, 353
BclI TGATCA 2 cut(s) 300, 387
BfaI CTAG 2 cut(s) 408, 501
BfrI CTTAAG 1 cut(s) 520
BisI GCNGC 2 cut(s) 40, 405
BlsI GCNGC 2 cut(s) 41, 406
BmcAI AGTACT 1 cut(s) 374
BmtI GCTAGC 1 cut(s) 411
BpuEI CTTGAG 1 cut(s) 353
BsaJI CCNNGG 3 cut(s) 145, 294, 509
Bsc4I CCNNNNNNNGG 2 cut(s) 418, 515
Bse3DI GCAATG 1 cut(s) 547
BseDI CCNNGG 3 cut(s) 145, 294, 509
BseLI CCNNNNNNNGG 2 cut(s) 418, 515
BseMI GCAATG 1 cut(s) 547
BseMII CTCAG 1 cut(s) 186
BseRI GAGGAG 1 cut(s) 160
BseSI GKGCMC 1 cut(s) 325
BseXI GCAGC 2 cut(s) 26, 391
BsgI GTGCAG 1 cut(s) 25
Bsh1236I CGCG 2 cut(s) 270, 464
BshFI GGCC 4 cut(s) 249, 331, 366, 470
BsiHKAI GWGCWC 1 cut(s) 83
BsiSI CCGG 1 cut(s) 162
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 2 cut(s) 418, 515
BsmFI GGGAC 1 cut(s) 257
BsnI GGCC 4 cut(s) 249, 331, 366, 470
Bsp1286I GDGCHC 2 cut(s) 83, 325
Bsp143I GATC 3 cut(s) 6, 300, 387
Bsp19I CCATGG 1 cut(s) 509
BspACI CCGC 1 cut(s) 464
BspANI GGCC 4 cut(s) 249, 331, 366, 470
BspCNI CTCAG 1 cut(s) 185
BspFNI CGCG 2 cut(s) 270, 464
BspOI GCTAGC 1 cut(s) 411
BspTI CTTAAG 1 cut(s) 520
BsrDI GCAATG 1 cut(s) 547
BssECI CCNNGG 3 cut(s) 145, 294, 509
BssMI GATC 3 cut(s) 6, 300, 387
BssT1I CCWWGG 1 cut(s) 509
Bst6I CTCTTC 1 cut(s) 145
BstAFI CTTAAG 1 cut(s) 520
BstC8I GCNNGC 1 cut(s) 409
BstDEI CTNAG 1 cut(s) 172
BstDSI CCRYGG 1 cut(s) 509
BstFNI CGCG 2 cut(s) 270, 464
BstHHI GCGC 1 cut(s) 540
BstKTI GATC 3 cut(s) 9, 303, 390
BstMBI GATC 3 cut(s) 6, 300, 387
BstMWI GCNNNNNNNGC 1 cut(s) 87
BstSLI GKGCMC 1 cut(s) 325
BstUI CGCG 2 cut(s) 270, 464
BstV1I GCAGC 2 cut(s) 26, 391
BsuRI GGCC 4 cut(s) 249, 331, 366, 470
BtgI CCRYGG 1 cut(s) 509
Cac8I GCNNGC 1 cut(s) 409
CfoI GCGC 1 cut(s) 540
CseI GACGC 2 cut(s) 259, 470
Csp6I GTAC 1 cut(s) 373
CspCI CAANNNNNGTGG 2 cut(s) 379, 414
CviAII CATG 3 cut(s) 214, 240, 510
CviQI GTAC 1 cut(s) 373
DdeI CTNAG 1 cut(s) 172
DpnI GATC 3 cut(s) 8, 302, 389
DpnII GATC 3 cut(s) 6, 300, 387
EaeI YGGCCR 2 cut(s) 247, 329
Eam1104I CTCTTC 1 cut(s) 145
EarI CTCTTC 1 cut(s) 145
Ecl136II GAGCTC 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 509
Eco147I AGGCCT 1 cut(s) 470
Eco24I GRGCYC 1 cut(s) 83
Eco53kI GAGCTC 1 cut(s) 81
EcoICRI GAGCTC 1 cut(s) 81
EcoT14I CCWWGG 1 cut(s) 509
EcoT38I GRGCYC 1 cut(s) 83
ErhI CCWWGG 1 cut(s) 509
FaeI CATG 3 cut(s) 217, 243, 513
FaiI YATR 9 cut(s) 119, 134, 215, 241, 260, 350, 457, 511, 562
FalI AAGNNNNNCTT 2 cut(s) 142, 174
FaqI GGGAC 1 cut(s) 257
FatI CATG 3 cut(s) 213, 239, 509
FbaI TGATCA 2 cut(s) 300, 387
Fnu4HI GCNGC 2 cut(s) 40, 405
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 2 cut(s) 40, 405
FspBI CTAG 2 cut(s) 408, 501
FspI TGCGCA 1 cut(s) 539
GlaI GCGC 1 cut(s) 539
GluI GCNGC 2 cut(s) 40, 405
HaeIII GGCC 4 cut(s) 249, 331, 366, 470
HapII CCGG 1 cut(s) 162
HgaI GACGC 2 cut(s) 259, 470
HhaI GCGC 1 cut(s) 540
Hin1II CATG 3 cut(s) 217, 243, 513
Hin6I GCGC 1 cut(s) 538
HinP1I GCGC 1 cut(s) 538
HinfI GANTC 1 cut(s) 153
HpaII CCGG 1 cut(s) 162
HphI GGTGA 3 cut(s) 139, 310, 334
Hpy188I TCNGA 5 cut(s) 72, 149, 175, 355, 387
Hpy99I CGWCG 1 cut(s) 464
HpyAV CCTTC 2 cut(s) 478, 489
HpyCH4V TGCA 1 cut(s) 42
HpyF10VI GCNNNNNNNGC 1 cut(s) 87
HpyF3I CTNAG 1 cut(s) 172
Hsp92II CATG 3 cut(s) 217, 243, 513
HspAI GCGC 1 cut(s) 538
Ksp22I TGATCA 2 cut(s) 300, 387
Kzo9I GATC 3 cut(s) 6, 300, 387
LmnI GCTCC 1 cut(s) 78
LpnPI CCDG 3 cut(s) 110, 175, 432
Lsp1109I GCAGC 2 cut(s) 26, 391
MaeI CTAG 2 cut(s) 408, 501
MaeIII GTNAC 1 cut(s) 154
MalI GATC 3 cut(s) 8, 302, 389
MboI GATC 3 cut(s) 6, 300, 387
MboII GAAGA 2 cut(s) 162, 245
MhlI GDGCHC 2 cut(s) 83, 325
MlsI TGGCCA 2 cut(s) 249, 331
MluCI AATT 5 cut(s) 139, 196, 277, 451, 550
MluNI TGGCCA 2 cut(s) 249, 331
MlyI GAGTC 1 cut(s) 162
MnlI CCTC 9 cut(s) 61, 155, 181, 213, 221, 304, 377, 460, 508
Mox20I TGGCCA 2 cut(s) 249, 331
MscI TGGCCA 2 cut(s) 249, 331
MseI TTAA 4 cut(s) 47, 86, 438, 521
MslI CAYNNNNRTG 1 cut(s) 224
Msp20I TGGCCA 2 cut(s) 249, 331
MspCI CTTAAG 1 cut(s) 520
MspI CCGG 1 cut(s) 162
MvnI CGCG 2 cut(s) 270, 464
MwoI GCNNNNNNNGC 1 cut(s) 87
NcoI CCATGG 1 cut(s) 509
NdeII GATC 3 cut(s) 6, 300, 387
NheI GCTAGC 1 cut(s) 407
NlaIII CATG 3 cut(s) 217, 243, 513
NmuCI GTSAC 1 cut(s) 154
NsbI TGCGCA 1 cut(s) 539
OliI CACNNNNGTG 1 cut(s) 224
PceI AGGCCT 1 cut(s) 470
PkrI GCNGC 2 cut(s) 41, 406
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
Psp124BI GAGCTC 1 cut(s) 83
RsaI GTAC 1 cut(s) 374
RsaNI GTAC 1 cut(s) 373
RseI CAYNNNNRTG 1 cut(s) 224
SacI GAGCTC 1 cut(s) 83
SaqAI TTAA 4 cut(s) 47, 86, 438, 521
SatI GCNGC 2 cut(s) 40, 405
Sau3AI GATC 3 cut(s) 6, 300, 387
ScaI AGTACT 1 cut(s) 374
SchI GAGTC 1 cut(s) 162
SduI GDGCHC 2 cut(s) 83, 325
SetI ASST 5 cut(s) 53, 83, 92, 147, 429
SmiMI CAYNNNNRTG 1 cut(s) 224
SmlI CTYRAG 2 cut(s) 368, 520
SmoI CTYRAG 2 cut(s) 368, 520
Sse9I AATT 5 cut(s) 139, 196, 277, 451, 550
SseBI AGGCCT 1 cut(s) 470
SsiI CCGC 1 cut(s) 464
SspMI CTAG 2 cut(s) 408, 501
SstI GAGCTC 1 cut(s) 83
StuI AGGCCT 1 cut(s) 470
StyI CCWWGG 1 cut(s) 509
TaqI TCGA 1 cut(s) 57
TasI AATT 5 cut(s) 139, 196, 277, 451, 550
TatI WGTACW 1 cut(s) 372
Tru1I TTAA 4 cut(s) 47, 86, 438, 521
Tru9I TTAA 4 cut(s) 47, 86, 438, 521
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 2 cut(s) 39, 404
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 3 cut(s) 106, 497, 549
Vha464I CTTAAG 1 cut(s) 520
XapI RAATTY 1 cut(s) 451
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
XspI CTAG 2 cut(s) 408, 501
ZrmI AGTACT 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.