RLG00000027655

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002588
Physical Location & Seq
Forward (+)
13629387 .. 13631165
1779 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027655

Sequence Viewer

Length: 1779 bp
ATGACGGTTTGCGGTCCCGGCAGATGGGCCGACACCAAAGGCATGACACTCCAGGTCCTCAACGGCCGGTGGTTCATGGTCTTTGCATGTCTTTTAATCATGTCCGCTGCCGGCGCCACCTACATGTTCAGCCTCTACTCTTCCGACATCAAGACCGTCCTCGGCTACGACCAAAGCACACTCAACCTCCTCAGCTTCTTCAAAGACTTCGGCTCCAACGTCGGCATCCTCTCCGGCCTCCTCAACGAGGTCACGCCACCGTGGGTGGTCCTCTTGGTCGGCGCCGTCCTCAACTTCTTCGGATACTTAATGATCTGGCTCTCCGTCACCAAGAAAGTCGCCAGACCAAAAGTCTGGCAGATGTGCCTCTACATTTGCATCGGCGCCAATTCCCAGTCGTTCGCCAACACCGGCTCGCTGGTCACGTGCGTCAAGAACTTCCCCGAGAGCCGTGGCGTCGTTTTGGGGCTCCTCAAGGGCTACGTCGGCCTCAGCGGCGCCATCATCACCCAGATGTATTTTGCTTTCTACTACAACGACACCAGATCCTTGATTTTGCTCATCGGGTGGCTTCCGGCGGCCATATCGTTCGCCTTTCTGAAGACTATTAGGATCATGAAGGTGATCAGACAAAGCAACGAGGTCAAGGTGTTCTACAACATGTTGTACATCTCTCTCGGGCTCGCTGGCTTTCTGATGATCATGATCATAGTACAGCATAAGGTGGACTTCACTCAAGGGGAGTACGGCGCCAGCACCGCCATGGTGCTCTTCTTGCTCGCCCTCCCCATCGTGGTGGTTGTAATTGAGGAGTACAAGATTTGGGAGAGCAAAGAACTAGAGATGAAGGGTGACGTCAAAATAGTCACTGAAGAGCCCGTCCCTGCTGCCGTCCCAACCGATAAACAATTAGTGGATGAAGCTCCTGAGTCCACCGCGGAACCTACCCAATCTTGTTGGAAAACTTGTTTTAGGCCACCAAATAGAGGGGAGGACTACACAATTCTCCAAGCCCTATTCAGCATTGACATGCTCATACTATTCATGGCGTGCATATGTGGTGTTGGGGGCACATTGACAGCAATTGACAACTTGGGTCAGATTGGGACTTCATTGGGATATGCCAAACGCAGCACGAGTACTTTCGTGTCCCTAGTCAGCATATGGAATTATCTCGGTCGAGTTGTTGCCGGATTCTACTCTGAATTCTTACTAAAACGATACAAATTCCCTCGTCCCCTCATTTTAACCCTAACCCTCTTGCTCTCTTGCGTTGGTCATCTCCTCATTGCCTTCAATGTTAAAAATGGCCTCTACCTGGCCTCAGTCATAATAGGGTTTTGCTTCGGCGCGCAATGGCCTTTGCTCTTCGCCATAATCTCTGAGCTCTTCGGGCTCAAGTACTACTCAACCTTGTACAATTTCGGTTCAGCAGCGAGCCCCGTCGGCCTGTACCTGCTCAACGTGAAACTCACCGGTCATGTGTACGATAAGGAGGCGAGGAAGCAATTGGCGGCTTTAGGGCTCCGTAGGAAAGAAGGGGAGCAGTTGGATTGCAATGGTGGAGAGTGCTTCAAGTTGTCCTTTATTATAATTGCAGCTGCCACATTTTTTGGGGCACTGGTTTCACTGATTTTAGTGATGAGGACAAGGAAGTTTTACCAGAGTGATATATACAAGAAGTTCAGAGAGGAGGCAGAAAAAAATGCTGAGACTGAAATGGCCGTGGGAGAAAATGGTGTCACCAATGGCCATCACCAAACCAATGAAAGATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 Nodulin-like

Protein Analysis

593

Amino Acids

65.74

Weight (kDa)

8.77

Isoelectric Point (pI)

35.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 19 - 168 1.7e-07 Major Facilitator Superfamily
Nodulin-like PF06813 23 - 268 9.6e-94 Nodulin-like
MFS_1 PF07690 256 - 480 3.2e-07 Major Facilitator Superfamily
NFD4_C PF23262 339 - 554 3.8e-19 NFD4, C-terminal domain
MFS_1 PF07690 354 - 550 8.6e-07 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1592
AatII GACGTC 1 cut(s) 858
Acc36I ACCTGC 1 cut(s) 1464
AccB1I GGYRCC 5 cut(s) 113, 281, 383, 497, 749
AccII CGCG 2 cut(s) 938, 1352
AciI CCGC 8 cut(s) 12, 105, 495, 578, 759, 936, 938, 1514
AclWI GGATC 2 cut(s) 540, 620
AcoI YGGCCR 4 cut(s) 64, 579, 1722, 1750
AcsI RAATTY 2 cut(s) 1205, 1226
AcuI CTGAAG 2 cut(s) 620, 891
AcvI CACGTG 1 cut(s) 426
AcyI GRCGYC 7 cut(s) 114, 282, 384, 456, 498, 750, 855
AfaI GTAC 9 cut(s) 668, 714, 746, 815, 1141, 1403, 1418, 1454, 1487
AfiI CCNNNNNNNGG 5 cut(s) 24, 247, 793, 986, 1318
AflIII ACRYGT 2 cut(s) 123, 660
AgeI ACCGGT 1 cut(s) 1475
AgsI TTSAA 3 cut(s) 202, 1297, 1576
AhdI GACNNNNNGTC 1 cut(s) 350
AjnI CCWGG 2 cut(s) 51, 1317
AluBI AGCT 4 cut(s) 195, 923, 1387, 1601
AluI AGCT 4 cut(s) 195, 923, 1387, 1601
Alw21I GWGCWC 2 cut(s) 771, 1389
Alw26I GTCTC 1 cut(s) 1706
AlwI GGATC 2 cut(s) 540, 620
Ama87I CYCGRG 2 cut(s) 443, 677
ApeKI GCWGC 6 cut(s) 107, 887, 1131, 1433, 1598, 1601
ApoI RAATTY 2 cut(s) 1205, 1226
AsiGI ACCGGT 1 cut(s) 1475
AspLEI GCGC 7 cut(s) 116, 284, 386, 500, 752, 1352, 1354
AspS9I GGNCC 4 cut(s) 14, 27, 55, 268
AsuC2I CCSGG 1 cut(s) 18
AsuHPI GGTGA 7 cut(s) 319, 499, 634, 863, 1465, 1735, 1748
AvaI CYCGRG 2 cut(s) 443, 677
AvaII GGWCC 3 cut(s) 14, 55, 268
BaeGI GKGCMC 2 cut(s) 1073, 1621
BalI TGGCCA 1 cut(s) 1752
BanI GGYRCC 5 cut(s) 113, 281, 383, 497, 749
BanII GRGCYC 7 cut(s) 471, 684, 879, 1389, 1398, 1442, 1527
BauI CACGAG 1 cut(s) 1135
BbrPI CACGTG 1 cut(s) 426
BbsI GAAGAC 1 cut(s) 608
Bbv12I GWGCWC 2 cut(s) 771, 1389
BbvCI CCTCAGC 2 cut(s) 191, 491
BbvI GCAGC 6 cut(s) 94, 874, 1143, 1445, 1588, 1610
BccI CCATC 4 cut(s) 18, 509, 797, 1761
BceAI ACGGC 6 cut(s) 79, 269, 435, 763, 875, 1709
BciT130I CCWGG 2 cut(s) 53, 1319
BciVI GTATCC 1 cut(s) 296
BclI TGATCA 3 cut(s) 624, 699, 705
BcnI CCSGG 1 cut(s) 18
BcoDI GTCTC 1 cut(s) 1706
BfaI CTAG 2 cut(s) 839, 1154
BfoI RGCGCY 5 cut(s) 117, 285, 387, 501, 753
BfuAI ACCTGC 1 cut(s) 1464
BfuI GTATCC 1 cut(s) 296
BglI GCCNNNNNGGC 2 cut(s) 495, 1446
BisI GCNGC 9 cut(s) 108, 496, 579, 888, 1132, 1434, 1515, 1599, 1602
BlsI GCNGC 9 cut(s) 109, 497, 580, 889, 1133, 1435, 1516, 1600, 1603
BmcAI AGTACT 2 cut(s) 1141, 1403
Bme1390I CCNGG 3 cut(s) 18, 53, 1319
Bme18I GGWCC 3 cut(s) 14, 55, 268
BmeRI GACNNNNNGTC 1 cut(s) 350
BmeT110I CYCGRG 2 cut(s) 443, 677
BmgT120I GGNCC 4 cut(s) 14, 27, 55, 268
BmrFI CCNGG 3 cut(s) 18, 53, 1319
BmrI ACTGGG 1 cut(s) 388
BmsI GCATC 2 cut(s) 234, 387
BmuI ACTGGG 1 cut(s) 388
BpiI GAAGAC 1 cut(s) 608
BpmI CTGGAG 1 cut(s) 35
Bpu10I CCTNAGC 2 cut(s) 191, 491
BpuEI CTTGAG 3 cut(s) 458, 720, 1382
BpuMI CCSGG 1 cut(s) 18
BsaAI YACGTR 1 cut(s) 426
BsaBI GATNNNNATC 1 cut(s) 704
BsaHI GRCGYC 7 cut(s) 114, 282, 384, 456, 498, 750, 855
BsaJI CCNNGG 6 cut(s) 160, 260, 451, 762, 936, 1725
BsaWI WCCGGW 1 cut(s) 1475
BsaXI ACNNNNNCTCC 4 cut(s) 171, 197, 201, 227
Bsc4I CCNNNNNNNGG 5 cut(s) 24, 247, 793, 986, 1318
Bse118I RCCGGY 4 cut(s) 66, 110, 410, 1475
Bse1I ACTGG 2 cut(s) 394, 1626
Bse3DI GCAATG 3 cut(s) 1287, 1361, 1564
Bse8I GATNNNNATC 1 cut(s) 704
BseBI CCWGG 2 cut(s) 53, 1319
BseDI CCNNGG 6 cut(s) 160, 260, 451, 762, 936, 1725
BseGI GGATG 2 cut(s) 225, 922
BseJI GATNNNNATC 1 cut(s) 704
BseLI CCNNNNNNNGG 5 cut(s) 24, 247, 793, 986, 1318
BseMI GCAATG 3 cut(s) 1287, 1361, 1564
BseMII CTCAG 6 cut(s) 205, 505, 918, 1338, 1374, 1701
BseNI ACTGG 2 cut(s) 394, 1626
BsePI GCGCGC 1 cut(s) 1350
BseRI GAGGAG 6 cut(s) 179, 230, 461, 824, 1274, 1706
BseSI GKGCMC 2 cut(s) 1073, 1621
BseX3I CGGCCG 1 cut(s) 64
BseXI GCAGC 6 cut(s) 94, 874, 1143, 1445, 1588, 1610
Bsh1236I CGCG 2 cut(s) 938, 1352
Bsh1285I CGRYCG 2 cut(s) 67, 1180
BshNI GGYRCC 5 cut(s) 113, 281, 383, 497, 749
BshTI ACCGGT 1 cut(s) 1475
BsiEI CGRYCG 2 cut(s) 67, 1180
BsiHKAI GWGCWC 2 cut(s) 771, 1389
BsiHKCI CYCGRG 2 cut(s) 443, 677
BsiSI CCGG 8 cut(s) 18, 67, 111, 234, 411, 575, 1191, 1476
BslFI GGGAC 5 cut(s) 866, 878, 1120, 1135, 1221
BslI CCNNNNNNNGG 5 cut(s) 24, 247, 793, 986, 1318
BsmAI GTCTC 1 cut(s) 1706
BsmFI GGGAC 5 cut(s) 866, 878, 1120, 1135, 1221
BsoBI CYCGRG 2 cut(s) 443, 677
Bsp1407I TGTACA 2 cut(s) 666, 1416
Bsp143I GATC 7 cut(s) 312, 545, 612, 624, 699, 705, 1772
Bsp19I CCATGG 1 cut(s) 762
BspACI CCGC 8 cut(s) 12, 105, 495, 578, 759, 936, 938, 1514
BspCNI CTCAG 6 cut(s) 204, 504, 919, 1337, 1375, 1702
BspFNI CGCG 2 cut(s) 938, 1352
BspHI TCATGA 2 cut(s) 615, 702
BspMI ACCTGC 1 cut(s) 1464
BspPI GGATC 2 cut(s) 540, 620
BspQI GCTCTTC 4 cut(s) 776, 867, 1373, 1394
BspT107I GGYRCC 5 cut(s) 113, 281, 383, 497, 749
BsrDI GCAATG 3 cut(s) 1287, 1361, 1564
BsrFI RCCGGY 4 cut(s) 66, 110, 410, 1475
BsrGI TGTACA 2 cut(s) 666, 1416
BsrI ACTGG 2 cut(s) 394, 1626
BssAI RCCGGY 4 cut(s) 66, 110, 410, 1475
BssECI CCNNGG 6 cut(s) 160, 260, 451, 762, 936, 1725
BssHII GCGCGC 1 cut(s) 1350
BssMI GATC 7 cut(s) 312, 545, 612, 624, 699, 705, 1772
BssNI GRCGYC 7 cut(s) 114, 282, 384, 456, 498, 750, 855
BssSI CACGAG 1 cut(s) 1135
BssT1I CCWWGG 1 cut(s) 762
Bst2BI CACGAG 1 cut(s) 1135
Bst2UI CCWGG 2 cut(s) 53, 1319
Bst4CI ACNGT 3 cut(s) 7, 157, 261
Bst6I CTCTTC 5 cut(s) 145, 776, 867, 1373, 1394
BstACI GRCGYC 7 cut(s) 114, 282, 384, 456, 498, 750, 855
BstAUI TGTACA 2 cut(s) 666, 1416
BstBAI YACGTR 1 cut(s) 426
BstC8I GCNNGC 9 cut(s) 112, 416, 684, 688, 754, 780, 1051, 1352, 1438
BstDEI CTNAG 6 cut(s) 191, 491, 927, 1324, 1383, 1710
BstDSI CCRYGG 5 cut(s) 260, 451, 762, 936, 1725
BstENI CCTNNNNNAGG 1 cut(s) 245
BstF5I GGATG 2 cut(s) 225, 922
BstFNI CGCG 2 cut(s) 938, 1352
BstH2I RGCGCY 5 cut(s) 117, 285, 387, 501, 753
BstHHI GCGC 7 cut(s) 116, 284, 386, 500, 752, 1352, 1354
BstKTI GATC 7 cut(s) 315, 548, 615, 627, 702, 708, 1775
BstMAI GTCTC 1 cut(s) 1706
BstMBI GATC 7 cut(s) 312, 545, 612, 624, 699, 705, 1772
BstMCI CGRYCG 2 cut(s) 67, 1180
BstMWI GCNNNNNNNGC 8 cut(s) 18, 113, 486, 495, 758, 775, 1393, 1446
BstNI CCWGG 2 cut(s) 53, 1319
BstNSI RCATGY 4 cut(s) 90, 127, 664, 1033
BstSCI CCNGG 3 cut(s) 16, 51, 1317
BstSLI GKGCMC 2 cut(s) 1073, 1621
BstUI CGCG 2 cut(s) 938, 1352
BstV1I GCAGC 6 cut(s) 94, 874, 1143, 1445, 1588, 1610
BstV2I GAAGAC 1 cut(s) 608
BstX2I RGATCY 1 cut(s) 545
BstXI CCANNNNNNTGG 2 cut(s) 354, 796
BstYI RGATCY 1 cut(s) 545
BstZI CGGCCG 1 cut(s) 64
BsuI GTATCC 1 cut(s) 296
BtgI CCRYGG 5 cut(s) 260, 451, 762, 936, 1725
BtsCI GGATG 2 cut(s) 225, 922
BtsIMutI CAGTG 3 cut(s) 867, 1619, 1628
BveI ACCTGC 1 cut(s) 1464
Cac8I GCNNGC 9 cut(s) 112, 416, 684, 688, 754, 780, 1051, 1352, 1438
CciI TCATGA 2 cut(s) 615, 702
CfoI GCGC 7 cut(s) 116, 284, 386, 500, 752, 1352, 1354
Cfr10I RCCGGY 4 cut(s) 66, 110, 410, 1475
Cfr13I GGNCC 4 cut(s) 14, 27, 55, 268
Cfr42I CCGCGG 1 cut(s) 939
CseI GACGC 2 cut(s) 418, 445
Csp6I GTAC 9 cut(s) 667, 713, 745, 814, 1140, 1402, 1417, 1453, 1486
CspAI ACCGGT 1 cut(s) 1475
CspCI CAANNNNNGTGG 2 cut(s) 1594, 1629
CviQI GTAC 9 cut(s) 667, 713, 745, 814, 1140, 1402, 1417, 1453, 1486
DdeI CTNAG 6 cut(s) 191, 491, 927, 1324, 1383, 1710
DinI GGCGCC 5 cut(s) 115, 283, 385, 499, 751
DpnI GATC 7 cut(s) 314, 547, 614, 626, 701, 707, 1774
DpnII GATC 7 cut(s) 312, 545, 612, 624, 699, 705, 1772
DriI GACNNNNNGTC 1 cut(s) 350
EaeI YGGCCR 4 cut(s) 64, 579, 1722, 1750
EagI CGGCCG 1 cut(s) 64
Eam1104I CTCTTC 5 cut(s) 145, 776, 867, 1373, 1394
Eam1105I GACNNNNNGTC 1 cut(s) 350
EarI CTCTTC 5 cut(s) 145, 776, 867, 1373, 1394
Ecl136II GAGCTC 1 cut(s) 1387
EclXI CGGCCG 1 cut(s) 64
Eco130I CCWWGG 1 cut(s) 762
Eco24I GRGCYC 7 cut(s) 471, 684, 879, 1389, 1398, 1442, 1527
Eco47I GGWCC 3 cut(s) 14, 55, 268
Eco52I CGGCCG 1 cut(s) 64
Eco53kI GAGCTC 1 cut(s) 1387
Eco57I CTGAAG 2 cut(s) 620, 891
Eco72I CACGTG 1 cut(s) 426
Eco88I CYCGRG 2 cut(s) 443, 677
EcoICRI GAGCTC 1 cut(s) 1387
EcoNI CCTNNNNNAGG 1 cut(s) 245
EcoO109I RGGNCCY 1 cut(s) 55
EcoRI GAATTC 1 cut(s) 1205
EcoRII CCWGG 2 cut(s) 51, 1317
EcoT14I CCWWGG 1 cut(s) 762
EcoT38I GRGCYC 7 cut(s) 471, 684, 879, 1389, 1398, 1442, 1527
EgeI GGCGCC 5 cut(s) 115, 283, 385, 499, 751
EheI GGCGCC 5 cut(s) 115, 283, 385, 499, 751
ErhI CCWWGG 1 cut(s) 762
FalI AAGNNNNNCTT 4 cut(s) 713, 745, 1568, 1600
FaqI GGGAC 5 cut(s) 866, 878, 1120, 1135, 1221
FauNDI CATATG 2 cut(s) 1055, 1163
FbaI TGATCA 3 cut(s) 624, 699, 705
Fnu4HI GCNGC 9 cut(s) 108, 496, 579, 888, 1132, 1434, 1515, 1599, 1602
FokI GGATG 2 cut(s) 212, 929
FriOI GRGCYC 7 cut(s) 471, 684, 879, 1389, 1398, 1442, 1527
Fsp4HI GCNGC 9 cut(s) 108, 496, 579, 888, 1132, 1434, 1515, 1599, 1602
FspBI CTAG 2 cut(s) 839, 1154
GlaI GCGC 7 cut(s) 115, 283, 385, 499, 751, 1351, 1353
GluI GCNGC 9 cut(s) 108, 496, 579, 888, 1132, 1434, 1515, 1599, 1602
GsuI CTGGAG 1 cut(s) 35
HaeII RGCGCY 5 cut(s) 117, 285, 387, 501, 753
HapII CCGG 8 cut(s) 18, 67, 111, 234, 411, 575, 1191, 1476
HgaI GACGC 2 cut(s) 418, 445
HhaI GCGC 7 cut(s) 116, 284, 386, 500, 752, 1352, 1354
Hin1I GRCGYC 7 cut(s) 114, 282, 384, 456, 498, 750, 855
Hin6I GCGC 7 cut(s) 114, 282, 384, 498, 750, 1350, 1352
HinP1I GCGC 7 cut(s) 114, 282, 384, 498, 750, 1350, 1352
HinfI GANTC 2 cut(s) 929, 1194
HpaII CCGG 8 cut(s) 18, 67, 111, 234, 411, 575, 1191, 1476
HphI GGTGA 7 cut(s) 319, 499, 634, 863, 1465, 1735, 1748
Hpy166II GTNNAC 3 cut(s) 727, 933, 1486
Hpy188I TCNGA 9 cut(s) 145, 302, 600, 629, 696, 1101, 1204, 1384, 1688
Hpy188III TCNNGA 5 cut(s) 151, 433, 616, 703, 926
Hpy8I GTNNAC 3 cut(s) 727, 933, 1486
Hpy99I CGWCG 4 cut(s) 224, 461, 488, 1448
HpyAV CCTTC 4 cut(s) 613, 841, 1303, 1532
HpyCH4III ACNGT 3 cut(s) 7, 157, 261
HpyCH4IV ACGT 5 cut(s) 219, 425, 483, 855, 1464
HpyCH4V TGCA 5 cut(s) 86, 378, 1053, 1557, 1598
HpyF10VI GCNNNNNNNGC 8 cut(s) 18, 113, 486, 495, 758, 775, 1393, 1446
HpyF3I CTNAG 6 cut(s) 191, 491, 927, 1324, 1383, 1710
HpySE526I ACGT 5 cut(s) 219, 425, 483, 855, 1464
Hsp92I GRCGYC 7 cut(s) 114, 282, 384, 456, 498, 750, 855
HspAI GCGC 7 cut(s) 114, 282, 384, 498, 750, 1350, 1352
KasI GGCGCC 5 cut(s) 113, 281, 383, 497, 749
KroI GCCGGC 1 cut(s) 110
KroNI GCCGGC 1 cut(s) 112
Ksp22I TGATCA 3 cut(s) 624, 699, 705
KspI CCGCGG 1 cut(s) 939
Kzo9I GATC 7 cut(s) 312, 545, 612, 624, 699, 705, 1772
LguI GCTCTTC 4 cut(s) 776, 867, 1373, 1394
LmnI GCTCC 5 cut(s) 218, 474, 928, 1530, 1543
Lsp1109I GCAGC 6 cut(s) 94, 874, 1143, 1445, 1588, 1610
LweI GCATC 2 cut(s) 234, 387
MaeI CTAG 2 cut(s) 839, 1154
MaeII ACGT 5 cut(s) 219, 425, 483, 855, 1464
MaeIII GTNAC 6 cut(s) 250, 325, 421, 851, 865, 1741
MalI GATC 7 cut(s) 314, 547, 614, 626, 701, 707, 1774
MboI GATC 7 cut(s) 312, 545, 612, 624, 699, 705, 1772
MboII GAAGA 8 cut(s) 132, 190, 289, 613, 763, 884, 1360, 1381
MfeI CAATTG 2 cut(s) 1083, 1508
MflI RGATCY 1 cut(s) 545
MlsI TGGCCA 1 cut(s) 1752
MluNI TGGCCA 1 cut(s) 1752
Mly113I GGCGCC 5 cut(s) 114, 282, 384, 498, 750
MlyI GAGTC 1 cut(s) 938
MmeI TCCRAC 4 cut(s) 168, 240, 938, 1530
Mox20I TGGCCA 1 cut(s) 1752
MroNI GCCGGC 1 cut(s) 110
MscI TGGCCA 1 cut(s) 1752
MseI TTAA 4 cut(s) 95, 308, 1247, 1302
MslI CAYNNNNRTG 6 cut(s) 122, 512, 620, 761, 794, 1028
Msp20I TGGCCA 1 cut(s) 1752
MspA1I CMGCKG 4 cut(s) 107, 495, 938, 1601
MspI CCGG 8 cut(s) 18, 67, 111, 234, 411, 575, 1191, 1476
MspR9I CCNGG 3 cut(s) 18, 53, 1319
MunI CAATTG 2 cut(s) 1083, 1508
MvaI CCWGG 2 cut(s) 53, 1319
MvnI CGCG 2 cut(s) 938, 1352
MwoI GCNNNNNNNGC 8 cut(s) 18, 113, 486, 495, 758, 775, 1393, 1446
NaeI GCCGGC 1 cut(s) 112
NarI GGCGCC 5 cut(s) 114, 282, 384, 498, 750
NciI CCSGG 1 cut(s) 18
NcoI CCATGG 1 cut(s) 762
NdeI CATATG 2 cut(s) 1055, 1163
NdeII GATC 7 cut(s) 312, 545, 612, 624, 699, 705, 1772
NgoMIV GCCGGC 1 cut(s) 110
NmeAIII GCCGAG 1 cut(s) 141
NmuCI GTSAC 6 cut(s) 250, 325, 421, 851, 865, 1741
NspI RCATGY 4 cut(s) 90, 127, 664, 1033
PagI TCATGA 2 cut(s) 615, 702
PauI GCGCGC 1 cut(s) 1350
PciI ACATGT 2 cut(s) 123, 660
PciSI GCTCTTC 4 cut(s) 776, 867, 1373, 1394
PcsI WCGNNNNNNNCGW 2 cut(s) 216, 422
PdiI GCCGGC 1 cut(s) 112
PfeI GAWTC 1 cut(s) 1194
PinAI ACCGGT 1 cut(s) 1475
PkrI GCNGC 9 cut(s) 109, 497, 580, 889, 1133, 1435, 1516, 1600, 1603
PleI GAGTC 1 cut(s) 937
PluTI GGCGCC 5 cut(s) 117, 285, 387, 501, 753
PmaCI CACGTG 1 cut(s) 426
PmlI CACGTG 1 cut(s) 426
PpsI GAGTC 1 cut(s) 937
Ppu21I YACGTR 1 cut(s) 426
PpuMI RGGWCCY 1 cut(s) 55
PscI ACATGT 2 cut(s) 123, 660
PsiI TTATAA 1 cut(s) 1592
Psp124BI GAGCTC 1 cut(s) 1389
Psp5II RGGWCCY 1 cut(s) 55
Psp6I CCWGG 2 cut(s) 51, 1317
PspCI CACGTG 1 cut(s) 426
PspGI CCWGG 2 cut(s) 51, 1317
PspPI GGNCC 4 cut(s) 14, 27, 55, 268
PspPPI RGGWCCY 1 cut(s) 55
PsuI RGATCY 1 cut(s) 545
PteI GCGCGC 1 cut(s) 1350
PvuII CAGCTG 1 cut(s) 1601
RsaI GTAC 9 cut(s) 668, 714, 746, 815, 1141, 1403, 1418, 1454, 1487
RsaNI GTAC 9 cut(s) 667, 713, 745, 814, 1140, 1402, 1417, 1453, 1486
RseI CAYNNNNRTG 6 cut(s) 122, 512, 620, 761, 794, 1028
SacI GAGCTC 1 cut(s) 1389
SacII CCGCGG 1 cut(s) 939
SapI GCTCTTC 4 cut(s) 776, 867, 1373, 1394
SaqAI TTAA 4 cut(s) 95, 308, 1247, 1302
SatI GCNGC 9 cut(s) 108, 496, 579, 888, 1132, 1434, 1515, 1599, 1602
Sau3AI GATC 7 cut(s) 312, 545, 612, 624, 699, 705, 1772
Sau96I GGNCC 4 cut(s) 14, 27, 55, 268
ScaI AGTACT 2 cut(s) 1141, 1403
SchI GAGTC 1 cut(s) 938
ScrFI CCNGG 3 cut(s) 18, 53, 1319
SfaNI GCATC 2 cut(s) 234, 387
SfoI GGCGCC 5 cut(s) 115, 283, 385, 499, 751
Sfr303I CCGCGG 1 cut(s) 939
SgrBI CCGCGG 1 cut(s) 939
SinI GGWCC 3 cut(s) 14, 55, 268
SmiMI CAYNNNNRTG 6 cut(s) 122, 512, 620, 761, 794, 1028
SmlI CTYRAG 3 cut(s) 473, 735, 1397
SmoI CTYRAG 3 cut(s) 473, 735, 1397
SsiI CCGC 8 cut(s) 12, 105, 495, 578, 759, 936, 938, 1514
SspDI GGCGCC 5 cut(s) 113, 281, 383, 497, 749
SspMI CTAG 2 cut(s) 839, 1154
SstI GAGCTC 1 cut(s) 1389
StyD4I CCNGG 3 cut(s) 16, 51, 1317
StyI CCWWGG 1 cut(s) 762
TaaI ACNGT 3 cut(s) 7, 157, 261
TaiI ACGT 5 cut(s) 222, 428, 486, 858, 1467
TaqI TCGA 1 cut(s) 1180
TaqII GACCGA 1 cut(s) 1166
TatI WGTACW 6 cut(s) 666, 712, 813, 1139, 1401, 1416
TauI GCSGC 3 cut(s) 498, 581, 1517
TfiI GAWTC 1 cut(s) 1194
Tru1I TTAA 4 cut(s) 95, 308, 1247, 1302
Tru9I TTAA 4 cut(s) 95, 308, 1247, 1302
TscAI CASTG 3 cut(s) 874, 1626, 1635
TseFI GTSAC 6 cut(s) 250, 325, 421, 851, 865, 1741
TseI GCWGC 6 cut(s) 107, 887, 1131, 1433, 1598, 1601
Tsp45I GTSAC 6 cut(s) 250, 325, 421, 851, 865, 1741
TspDTI ATGAA 6 cut(s) 64, 632, 860, 933, 1033, 1101
TspGWI ACGGA 2 cut(s) 313, 1517
TspRI CASTG 3 cut(s) 874, 1626, 1635
VpaK11BI GGWCC 3 cut(s) 14, 55, 268
XagI CCTNNNNNAGG 1 cut(s) 245
XapI RAATTY 2 cut(s) 1205, 1226
XceI RCATGY 4 cut(s) 90, 127, 664, 1033
XcmI CCANNNNNNNNNTGG 1 cut(s) 760
XspI CTAG 2 cut(s) 839, 1154
ZraI GACGTC 1 cut(s) 856
ZrmI AGTACT 2 cut(s) 1141, 1403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.