Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0002588

Physical Location & Seq

Genomic Coordinates Reverse (-)

24253626 .. 24255422

Gene Structure 1797 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1168000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1797 bp

ATGTTGGTCTCTGGACCTGGTGGAGGAGGGGGATGGAGCAACACCAAAACCCTAGCCCTCCAAGTCCTCTATGGGAGGTGGTTCATGATGTTTGCTTCCCTCTTAATCATGTCAGCCGCTGGCGCCACCTACATGTTTGGTCTCTACTCCGGTGACATCAAAGATGTGCTGGGGTATGACCAAAGTACCCTCAATCTTCTCAGCTTCTTCAAGGACTTGGGGTCTAATGTTGGTGTCCTTTCTGGTCTAATCAACGAGGTAACACCCCCATGGGTGGTCCTATCAGTGGGTGGAGTCCTCAATTTTTTTGGTTACTTCATGATTTGGATGGCCGTGACCAAAAGAATTGCAAGGCCTCAGGTTTGGCAGATGTGCCTGTACATCTGTATAGGTGCAAATTCTCAGTCTTTTGCCAATACCGGATCACTTGTCACTTGTGTCAAAAACTTCCCGGAGAGCCGTGGCGCGGTTTTGGGAATCTTGAAGGGATATGTTGGCCTAAGTGGTGCTATATTCACTCAATTATACTCTGCTTTTTATTATAATGACACCAAGGCTTTGATTTTGTTAATTGCGTGGCTTCCTGCTGCCATTTCTTTTGTTTTTCTTAGAATAATTCGGATTATGAAGGTCATTAGACATCCCAATGAAGTCAAAGTGTTTTATAATATGCTTTATATCTCACTTGGGCTTGCTGGATTTTTAATGGTTATGATTATAATCCAGAAGAATGTTCATTTCACTCAAGGCGAGTATGGTGCTAGTGCTGCCATGGTTATTTTCTTGCTTGTCCTCCCACTTGCCGTGGTTATTATTGAGGAATTTAAGATCTTGGAAGGCAAGAAGCTCGAGTTGAATGGCAGTTCGGACTTGAAGATAGTTACCGAAAAGCCTAAATCAGAAGAAGAAAGCGTTCCATCCGATATGTCACGACCCAGGGAAGCGCTCGCCACCACTACCACAGAGACTGAACCGTCGTGCTGGAAAACTATGTTTAGGCCACCAGATAGAGGTGAGGACTACACCATACTCCAAGCACTTTTCAGCATTGACATGCTCATTCTTTTCATTGCATGCATATGTGGTGTTGGTGGCACATTGACCGCAATAGACAACTTGGGTCAGATTGGAGCTTCTTTAGGGTACCCTAAACAAAGCATAAGCACTTTTGTGTCCCTAGTAAGCATATGGAATTATTTGGGTCGTGTCGTGTCGGGTTTCGGCTCCGAAATCTTGTTGAAAAGATACAAGTTCCCTCGTCCTCTGATGTTAACCCTAACCCTCTTGCTTTCTTGTGTTGGCCACCTTCTCGTTGCCTTCAATGTCAAAAATGGTCTCTACCTAGCCTCCGTGATCATAGGGTTTTGTTTCGGTGCCCAATGGCCTCTACTTTTTGCCATAATTTCTGAGCTTTTCGGCCTCAAATACTACTCCACTTTGTATAATTTTGGGTCGTCTGCTAGCCCGGTTGGACTGTACTTGCTCAACGTGAGACTCACCGGGCACGTATATGACAAAGAAGCCAAAAAGCAGCTTGCAGCAAAAGGGCTTGTAAGACAAGCAGGCAAGGAGTTGACCTGCGTTGGGGGAGAGTGCTTCAAATTGTCCTTCATTATAATTGCAGCAGCCACATTATTTGGCACGTTCGTGTCACTGATTTTGGTGATGAGAACGAGAAAGTTTTACCAGAGTGATATATACAAGAAGTTCAGACAGGAAACAGAAGCTGCTGAGAGAGAGATGGCCACGGTAGATCAAAATGGTGCCAAACCATTGGAAACCAATGGAAGATCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MFS_1 Nodulin-like

Protein Analysis

599

Amino Acids

65.87

Weight (kDa)

9.1

Isoelectric Point (pI)

33.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MFS_1	PF07690	19 - 172	6e-06	Major Facilitator Superfamily
Nodulin-like	PF06813	26 - 271	3.2e-95	Nodulin-like
MFS_1	PF07690	309 - 487	1e-08	Major Facilitator Superfamily
NFD4_C	PF23262	347 - 562	2.1e-16	NFD4, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002588)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39210
fragaria_vesca	FvH4_6g16630 FvH4_7g18510
malus_domestica	MD01G1101400.v1.1 MD04G1090400.v1.1 MD07G1168000.v1.1
prunus_persica	Prupe.2G206600_v2.0.a1 Prupe.6G220200_v2.0.a1 Prupe.7G127100_v2.0.a1
pyrus_communis	pycom07g16250 pycom12g10870
rosa_chinensis	RchiOBHm_Chr1g0334871 RchiOBHm_Chr1g0362291 RchiOBHm_Chr3g0470591
rosa_laevigata	RLG00000024257 RLG00000027655 RLG00000029413
rosa_multiflora	Rmu_co8175752.1_g000001 Rmu_sc0000574.1_g000047 Rmu_sc0002115.1_g000014 Rmu_sc0007125.1_g000010 Rmu_ssc0000009.1_g000029
rosa_roxburghii	Rroxscaffold_4G00294280 Rroxscaffold_6G00410710
rosa_rugosa	Rorug01G0117400 Rorug01G0297200 Rorug03G0110100.1
rosa_samantha	Rh1AG306000 Rh1BG108600 Rh1BG269500 Rh1CG287000 Rh1DG145500 Rh1DG299600 Rh3AG160300 Rh3BG184800 Rh3CG175300 Rh3DG189900
rosa_wichuraiana	Rw1G011680 Rw1G027150 Rw3G015160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	543, 666, 719, 1616
AasI	GACNNNNNNGTC	1 cut(s)	973
Acc36I	ACCTGC	1 cut(s)	1586
Acc65I	GGTACC	1 cut(s)	1143
AccB1I	GGYRCC	4 cut(s)	122, 1143, 1373, 1763
AccII	CGCG	1 cut(s)	467
AciI	CCGC	3 cut(s)	117, 467, 1104
AclWI	GGATC	1 cut(s)	430
AcoI	YGGCCR	3 cut(s)	330, 1300, 1743
AcsI	RAATTY	2 cut(s)	397, 821
AcyI	GRCGYC	1 cut(s)	123
AfaI	GTAC	4 cut(s)	187, 380, 1145, 1478
AfeI	AGCGCT	1 cut(s)	945
AfiI	CCNNNNNNNGG	6 cut(s)	23, 274, 286, 466, 1010, 1584
AflIII	ACRYGT	1 cut(s)	132
AgsI	TTSAA	7 cut(s)	211, 484, 856, 874, 1240, 1321, 1600
AhdI	GACNNNNNGTC	1 cut(s)	220
AjnI	CCWGG	2 cut(s)	16, 935
AleI	CACNNNNGTG	2 cut(s)	1169, 1646
AluBI	AGCT	6 cut(s)	204, 847, 1133, 1411, 1534, 1727
AluI	AGCT	6 cut(s)	204, 847, 1133, 1411, 1534, 1727
Alw26I	GTCTC	5 cut(s)	13, 146, 959, 1340, 1486
AlwI	GGATC	1 cut(s)	430
AlwNI	CAGNNNCTG	3 cut(s)	119, 968, 1727
Ama87I	CYCGRG	1 cut(s)	848
Aor51HI	AGCGCT	1 cut(s)	945
AoxI	GGCC	8 cut(s)	330, 353, 496, 998, 1300, 1382, 1417, 1743
ApeKI	GCWGC	7 cut(s)	587, 767, 1531, 1538, 1622, 1625, 1727
ApoI	RAATTY	2 cut(s)	397, 821
ArsI	GACNNNNNNTTYG	2 cut(s)	389, 421
Asp700I	GAANNNNTTC	1 cut(s)	912
Asp718I	GGTACC	1 cut(s)	1143
AspLEI	GCGC	3 cut(s)	125, 467, 946
AspS9I	GGNCC	2 cut(s)	14, 277
AsuC2I	CCSGG	3 cut(s)	452, 1466, 1501
AsuHPI	GGTGA	4 cut(s)	164, 1025, 1489, 1675
AsuNHI	GCTAGC	1 cut(s)	1460
AvaI	CYCGRG	1 cut(s)	848
AvaII	GGWCC	2 cut(s)	14, 277
AxyI	CCTNAGG	1 cut(s)	357
BaeGI	GKGCMC	2 cut(s)	1378, 1506
BaeI	ACNNNNGTAYC	2 cut(s)	169, 202
BalI	TGGCCA	2 cut(s)	1302, 1745
BanI	GGYRCC	4 cut(s)	122, 1143, 1373, 1763
BbvI	GCAGC	7 cut(s)	574, 754, 1543, 1550, 1634, 1637, 1714
BccI	CCATC	4 cut(s)	27, 322, 925, 1735
BceAI	ACGGC	3 cut(s)	317, 444, 788
BcgI	CGANNNNNNTGC	4 cut(s)	740, 774, 829, 863
BciT130I	CCWGG	2 cut(s)	18, 937
BclI	TGATCA	1 cut(s)	1353
BcnI	CCSGG	3 cut(s)	452, 1466, 1501
BcoDI	GTCTC	5 cut(s)	13, 146, 959, 1340, 1486
BfaI	CTAG	5 cut(s)	53, 762, 1178, 1343, 1461
BfoI	RGCGCY	2 cut(s)	126, 947
BfuAI	ACCTGC	1 cut(s)	1586
BglII	AGATCT	1 cut(s)	828
BisI	GCNGC	8 cut(s)	117, 588, 768, 1532, 1539, 1623, 1626, 1728
BlsI	GCNGC	8 cut(s)	118, 589, 769, 1533, 1540, 1624, 1627, 1729
Bme1390I	CCNGG	5 cut(s)	18, 452, 937, 1466, 1501
Bme18I	GGWCC	2 cut(s)	14, 277
BmeRI	GACNNNNNGTC	1 cut(s)	220
BmeT110I	CYCGRG	1 cut(s)	848
BmgT120I	GGNCC	2 cut(s)	14, 277
BmiI	GGNNCC	5 cut(s)	124, 1145, 1225, 1375, 1765
BmrFI	CCNGG	5 cut(s)	18, 452, 937, 1466, 1501
BmtI	GCTAGC	1 cut(s)	1464
BpuEI	CTTGAG	1 cut(s)	729
BpuMI	CCSGG	3 cut(s)	452, 1466, 1501
BsaAI	YACGTR	1 cut(s)	1507
BsaBI	GATNNNNATC	1 cut(s)	719
BsaHI	GRCGYC	1 cut(s)	123
BsaI	GGTCTC	3 cut(s)	13, 146, 1340
BsaJI	CCNNGG	8 cut(s)	269, 460, 552, 771, 804, 935, 936, 1746
BsaWI	WCCGGW	2 cut(s)	149, 419
BsaXI	ACNNNNNCTCC	2 cut(s)	1331, 1361
Bsc4I	CCNNNNNNNGG	6 cut(s)	23, 274, 286, 466, 1010, 1584
Bse21I	CCTNAGG	1 cut(s)	357
Bse3DI	GCAATG	1 cut(s)	1068
Bse8I	GATNNNNATC	1 cut(s)	719
BseBI	CCWGG	2 cut(s)	18, 937
BseDI	CCNNGG	8 cut(s)	269, 460, 552, 771, 804, 935, 936, 1746
BseGI	GGATG	4 cut(s)	38, 333, 640, 917
BseJI	GATNNNNATC	1 cut(s)	719
BseLI	CCNNNNNNNGG	6 cut(s)	23, 274, 286, 466, 1010, 1584
BseMI	GCAATG	1 cut(s)	1068
BseMII	CTCAG	5 cut(s)	214, 371, 416, 1398, 1722
BseRI	GAGGAG	1 cut(s)	39
BseSI	GKGCMC	2 cut(s)	1378, 1506
BseXI	GCAGC	7 cut(s)	574, 754, 1543, 1550, 1634, 1637, 1714
BseYI	CCCAGC	1 cut(s)	169
Bsh1236I	CGCG	1 cut(s)	467
BshFI	GGCC	8 cut(s)	332, 355, 498, 1000, 1302, 1384, 1419, 1745
BshNI	GGYRCC	4 cut(s)	122, 1143, 1373, 1763
BsiHKCI	CYCGRG	1 cut(s)	848
BsiSI	CCGG	5 cut(s)	150, 420, 452, 1466, 1500
BslFI	GGGAC	1 cut(s)	1159
BslI	CCNNNNNNNGG	6 cut(s)	23, 274, 286, 466, 1010, 1584
BsmAI	GTCTC	5 cut(s)	13, 146, 959, 1340, 1486
BsmFI	GGGAC	1 cut(s)	1159
BsnI	GGCC	8 cut(s)	332, 355, 498, 1000, 1302, 1384, 1419, 1745
Bso31I	GGTCTC	3 cut(s)	13, 146, 1340
BsoBI	CYCGRG	1 cut(s)	848
Bsp1286I	GDGCHC	2 cut(s)	1378, 1506
Bsp1407I	TGTACA	1 cut(s)	378
Bsp143I	GATC	5 cut(s)	422, 828, 1353, 1753, 1790
Bsp19I	CCATGG	2 cut(s)	269, 771
BspACI	CCGC	3 cut(s)	117, 467, 1104
BspANI	GGCC	8 cut(s)	332, 355, 498, 1000, 1302, 1384, 1419, 1745
BspCNI	CTCAG	5 cut(s)	213, 370, 415, 1399, 1723
BspFNI	CGCG	1 cut(s)	467
BspHI	TCATGA	2 cut(s)	84, 318
BspLI	GGNNCC	5 cut(s)	124, 1145, 1225, 1375, 1765
BspMI	ACCTGC	1 cut(s)	1586
BspOI	GCTAGC	1 cut(s)	1464
BspPI	GGATC	1 cut(s)	430
BspT107I	GGYRCC	4 cut(s)	122, 1143, 1373, 1763
BspTNI	GGTCTC	3 cut(s)	13, 146, 1340
BsrDI	GCAATG	1 cut(s)	1068
BsrGI	TGTACA	1 cut(s)	378
BssECI	CCNNGG	8 cut(s)	269, 460, 552, 771, 804, 935, 936, 1746
BssMI	GATC	5 cut(s)	422, 828, 1353, 1753, 1790
BssNI	GRCGYC	1 cut(s)	123
BssT1I	CCWWGG	3 cut(s)	269, 552, 771
Bst2UI	CCWGG	2 cut(s)	18, 937
Bst4CI	ACNGT	3 cut(s)	975, 1476, 1750
BstACI	GRCGYC	1 cut(s)	123
BstAUI	TGTACA	1 cut(s)	378
BstBAI	YACGTR	1 cut(s)	1507
BstC8I	GCNNGC	7 cut(s)	121, 693, 948, 1075, 1462, 1536, 1564
BstDEI	CTNAG	7 cut(s)	200, 357, 402, 500, 608, 1407, 1731
BstDSI	CCRYGG	5 cut(s)	269, 460, 771, 804, 1746
BstENI	CCTNNNNNAGG	1 cut(s)	21
BstF5I	GGATG	4 cut(s)	38, 333, 640, 917
BstFNI	CGCG	1 cut(s)	467
BstH2I	RGCGCY	2 cut(s)	126, 947
BstHHI	GCGC	3 cut(s)	125, 467, 946
BstKTI	GATC	5 cut(s)	425, 831, 1356, 1756, 1793
BstMAI	GTCTC	5 cut(s)	13, 146, 959, 1340, 1486
BstMBI	GATC	5 cut(s)	422, 828, 1353, 1753, 1790
BstMWI	GCNNNNNNNGC	2 cut(s)	122, 767
BstNI	CCWGG	2 cut(s)	18, 937
BstNSI	RCATGY	3 cut(s)	136, 1057, 1077
BstSCI	CCNGG	5 cut(s)	16, 450, 935, 1464, 1499
BstSLI	GKGCMC	2 cut(s)	1378, 1506
BstUI	CGCG	1 cut(s)	467
BstV1I	GCAGC	7 cut(s)	574, 754, 1543, 1550, 1634, 1637, 1714
BstX2I	RGATCY	1 cut(s)	828
BstXI	CCANNNNNNTGG	1 cut(s)	1774
BstYI	RGATCY	1 cut(s)	828
Bsu36I	CCTNAGG	1 cut(s)	357
BsuRI	GGCC	8 cut(s)	332, 355, 498, 1000, 1302, 1384, 1419, 1745
BtgI	CCRYGG	5 cut(s)	269, 460, 771, 804, 1746
BtsCI	GGATG	4 cut(s)	38, 333, 640, 917
BtsIMutI	CAGTG	2 cut(s)	291, 1652
BveI	ACCTGC	1 cut(s)	1586
Cac8I	GCNNGC	7 cut(s)	121, 693, 948, 1075, 1462, 1536, 1564
CaiI	CAGNNNCTG	3 cut(s)	119, 968, 1727
CciI	TCATGA	2 cut(s)	84, 318
CfoI	GCGC	3 cut(s)	125, 467, 946
Cfr13I	GGNCC	2 cut(s)	14, 277
CsiI	ACCWGGT	1 cut(s)	16
Csp6I	GTAC	4 cut(s)	186, 379, 1144, 1477
CspCI	CAANNNNNGTGG	2 cut(s)	1618, 1653
CviAII	CATG	8 cut(s)	85, 109, 133, 270, 319, 772, 1054, 1074
CviQI	GTAC	4 cut(s)	186, 379, 1144, 1477
DdeI	CTNAG	7 cut(s)	200, 357, 402, 500, 608, 1407, 1731
DinI	GGCGCC	1 cut(s)	124
DpnI	GATC	5 cut(s)	424, 830, 1355, 1755, 1792
DpnII	GATC	5 cut(s)	422, 828, 1353, 1753, 1790
DrdI	GACNNNNNNGTC	1 cut(s)	973
DriI	GACNNNNNGTC	1 cut(s)	220
DseDI	GACNNNNNNGTC	1 cut(s)	973
EaeI	YGGCCR	3 cut(s)	330, 1300, 1743
Eam1105I	GACNNNNNGTC	1 cut(s)	220
Eco130I	CCWWGG	3 cut(s)	269, 552, 771
Eco147I	AGGCCT	1 cut(s)	355
Eco31I	GGTCTC	3 cut(s)	13, 146, 1340
Eco47I	GGWCC	2 cut(s)	14, 277
Eco47III	AGCGCT	1 cut(s)	945
Eco81I	CCTNAGG	1 cut(s)	357
Eco88I	CYCGRG	1 cut(s)	848
EcoNI	CCTNNNNNAGG	1 cut(s)	21
EcoRII	CCWGG	2 cut(s)	16, 935
EcoT14I	CCWWGG	3 cut(s)	269, 552, 771
EcoT22I	ATGCAT	1 cut(s)	1079
EgeI	GGCGCC	1 cut(s)	124
EheI	GGCGCC	1 cut(s)	124
ErhI	CCWWGG	3 cut(s)	269, 552, 771
FaeI	CATG	8 cut(s)	88, 112, 136, 273, 322, 775, 1057, 1077
FaqI	GGGAC	1 cut(s)	1159
FatI	CATG	8 cut(s)	84, 108, 132, 269, 318, 771, 1053, 1073
FauNDI	CATATG	2 cut(s)	1079, 1187
FbaI	TGATCA	1 cut(s)	1353
Fnu4HI	GCNGC	8 cut(s)	117, 588, 768, 1532, 1539, 1623, 1626, 1728
FokI	GGATG	4 cut(s)	45, 340, 627, 904
Fsp4HI	GCNGC	8 cut(s)	117, 588, 768, 1532, 1539, 1623, 1626, 1728
FspBI	CTAG	5 cut(s)	53, 762, 1178, 1343, 1461
GlaI	GCGC	3 cut(s)	124, 466, 945
GluI	GCNGC	8 cut(s)	117, 588, 768, 1532, 1539, 1623, 1626, 1728
GsaI	CCCAGC	1 cut(s)	173
HaeII	RGCGCY	2 cut(s)	126, 947
HaeIII	GGCC	8 cut(s)	332, 355, 498, 1000, 1302, 1384, 1419, 1745
HapII	CCGG	5 cut(s)	150, 420, 452, 1466, 1500
HhaI	GCGC	3 cut(s)	125, 467, 946
Hin1I	GRCGYC	1 cut(s)	123
Hin1II	CATG	8 cut(s)	88, 112, 136, 273, 322, 775, 1057, 1077
Hin6I	GCGC	3 cut(s)	123, 465, 944
HinP1I	GCGC	3 cut(s)	123, 465, 944
HincII	GTYRAC	2 cut(s)	1272, 1575
HindII	GTYRAC	2 cut(s)	1272, 1575
HinfI	GANTC	3 cut(s)	294, 477, 1494
HpaI	GTTAAC	1 cut(s)	1272
HpaII	CCGG	5 cut(s)	150, 420, 452, 1466, 1500
HphI	GGTGA	4 cut(s)	164, 1025, 1489, 1675
Hpy166II	GTNNAC	2 cut(s)	1272, 1575
Hpy188I	TCNGA	9 cut(s)	621, 868, 901, 922, 1125, 1228, 1266, 1408, 1712
Hpy188III	TCNNGA	6 cut(s)	12, 85, 319, 481, 724, 930
Hpy8I	GTNNAC	2 cut(s)	1272, 1575
Hpy99I	CGWCG	1 cut(s)	979
HpyAV	CCTTC	6 cut(s)	478, 622, 830, 1316, 1327, 1618
HpyCH4III	ACNGT	3 cut(s)	975, 1476, 1750
HpyCH4IV	ACGT	3 cut(s)	1488, 1506, 1643
HpyCH4V	TGCA	6 cut(s)	350, 395, 1073, 1077, 1538, 1622
HpyF10VI	GCNNNNNNNGC	2 cut(s)	122, 767
HpyF3I	CTNAG	7 cut(s)	200, 357, 402, 500, 608, 1407, 1731
HpySE526I	ACGT	3 cut(s)	1488, 1506, 1643
Hsp92I	GRCGYC	1 cut(s)	123
Hsp92II	CATG	8 cut(s)	88, 112, 136, 273, 322, 775, 1057, 1077
HspAI	GCGC	3 cut(s)	123, 465, 944
KasI	GGCGCC	1 cut(s)	122
KpnI	GGTACC	1 cut(s)	1147
Ksp22I	TGATCA	1 cut(s)	1353
KspAI	GTTAAC	1 cut(s)	1272
Kzo9I	GATC	5 cut(s)	422, 828, 1353, 1753, 1790
LmnI	GCTCC	3 cut(s)	36, 1130, 1229
Lsp1109I	GCAGC	7 cut(s)	574, 754, 1543, 1550, 1634, 1637, 1714
MabI	ACCWGGT	1 cut(s)	16
MaeI	CTAG	5 cut(s)	53, 762, 1178, 1343, 1461
MaeII	ACGT	3 cut(s)	1488, 1506, 1643
MaeIII	GTNAC	8 cut(s)	152, 259, 311, 334, 430, 880, 927, 1650
MalI	GATC	5 cut(s)	424, 830, 1355, 1755, 1792
MboI	GATC	5 cut(s)	422, 828, 1353, 1753, 1790
MboII	GAAGA	6 cut(s)	188, 199, 739, 886, 914, 917
MflI	RGATCY	1 cut(s)	828
MhlI	GDGCHC	2 cut(s)	1378, 1506
MlsI	TGGCCA	2 cut(s)	1302, 1745
MluNI	TGGCCA	2 cut(s)	1302, 1745
Mly113I	GGCGCC	1 cut(s)	123
MlyI	GAGTC	2 cut(s)	303, 1488
MmeI	TCCRAC	1 cut(s)	1450
Mox20I	TGGCCA	2 cut(s)	1302, 1745
Mph1103I	ATGCAT	1 cut(s)	1079
MroXI	GAANNNNTTC	1 cut(s)	912
MscI	TGGCCA	2 cut(s)	1302, 1745
MseI	TTAA	5 cut(s)	104, 569, 704, 825, 1271
MslI	CAYNNNNRTG	8 cut(s)	131, 268, 645, 1052, 1078, 1169, 1509, 1646
Msp20I	TGGCCA	2 cut(s)	1302, 1745
MspA1I	CMGCKG	1 cut(s)	119
MspI	CCGG	5 cut(s)	150, 420, 452, 1466, 1500
MspR9I	CCNGG	5 cut(s)	18, 452, 937, 1466, 1501
MvaI	CCWGG	2 cut(s)	18, 937
MvnI	CGCG	1 cut(s)	467
MwoI	GCNNNNNNNGC	2 cut(s)	122, 767
NarI	GGCGCC	1 cut(s)	123
NciI	CCSGG	3 cut(s)	452, 1466, 1501
NcoI	CCATGG	2 cut(s)	269, 771
NdeI	CATATG	2 cut(s)	1079, 1187
NdeII	GATC	5 cut(s)	422, 828, 1353, 1753, 1790
NheI	GCTAGC	1 cut(s)	1460
NlaIII	CATG	8 cut(s)	88, 112, 136, 273, 322, 775, 1057, 1077
NlaIV	GGNNCC	5 cut(s)	124, 1145, 1225, 1375, 1765
NmuCI	GTSAC	5 cut(s)	152, 334, 430, 927, 1650
NsiI	ATGCAT	1 cut(s)	1079
NspI	RCATGY	3 cut(s)	136, 1057, 1077
OliI	CACNNNNGTG	2 cut(s)	1169, 1646
PaeI	GCATGC	1 cut(s)	1077
PaeR7I	CTCGAG	1 cut(s)	848
PagI	TCATGA	2 cut(s)	84, 318
PasI	CCCWGGG	1 cut(s)	936
PceI	AGGCCT	1 cut(s)	355
PciI	ACATGT	1 cut(s)	132
PdmI	GAANNNNTTC	1 cut(s)	912
PfeI	GAWTC	1 cut(s)	477
PfoI	TCCNGGA	1 cut(s)	450
PkrI	GCNGC	8 cut(s)	118, 589, 769, 1533, 1540, 1624, 1627, 1729
PleI	GAGTC	2 cut(s)	302, 1488
PluTI	GGCGCC	1 cut(s)	126
PpsI	GAGTC	2 cut(s)	302, 1488
Ppu21I	YACGTR	1 cut(s)	1507
PscI	ACATGT	1 cut(s)	132
PsiI	TTATAA	4 cut(s)	543, 666, 719, 1616
Psp6I	CCWGG	2 cut(s)	16, 935
PspFI	CCCAGC	1 cut(s)	169
PspGI	CCWGG	2 cut(s)	16, 935
PspN4I	GGNNCC	5 cut(s)	124, 1145, 1225, 1375, 1765
PspPI	GGNCC	2 cut(s)	14, 277
PspXI	VCTCGAGB	1 cut(s)	848
PstNI	CAGNNNCTG	3 cut(s)	119, 968, 1727
PsuI	RGATCY	1 cut(s)	828
RsaI	GTAC	4 cut(s)	187, 380, 1145, 1478
RsaNI	GTAC	4 cut(s)	186, 379, 1144, 1477
RseI	CAYNNNNRTG	8 cut(s)	131, 268, 645, 1052, 1078, 1169, 1509, 1646
SaqAI	TTAA	5 cut(s)	104, 569, 704, 825, 1271
SatI	GCNGC	8 cut(s)	117, 588, 768, 1532, 1539, 1623, 1626, 1728
Sau3AI	GATC	5 cut(s)	422, 828, 1353, 1753, 1790
Sau96I	GGNCC	2 cut(s)	14, 277
SchI	GAGTC	2 cut(s)	303, 1488
ScrFI	CCNGG	5 cut(s)	18, 452, 937, 1466, 1501
SduI	GDGCHC	2 cut(s)	1378, 1506
SexAI	ACCWGGT	1 cut(s)	16
SfoI	GGCGCC	1 cut(s)	124
Sfr274I	CTCGAG	1 cut(s)	848
SinI	GGWCC	2 cut(s)	14, 277
SlaI	CTCGAG	1 cut(s)	848
SmiMI	CAYNNNNRTG	8 cut(s)	131, 268, 645, 1052, 1078, 1169, 1509, 1646
SmlI	CTYRAG	2 cut(s)	744, 848
SmoI	CTYRAG	2 cut(s)	744, 848
SphI	GCATGC	1 cut(s)	1077
SseBI	AGGCCT	1 cut(s)	355
SsiI	CCGC	3 cut(s)	117, 467, 1104
SspDI	GGCGCC	1 cut(s)	122
SspMI	CTAG	5 cut(s)	53, 762, 1178, 1343, 1461
StuI	AGGCCT	1 cut(s)	355
StyD4I	CCNGG	5 cut(s)	16, 450, 935, 1464, 1499
StyI	CCWWGG	3 cut(s)	269, 552, 771
TaaI	ACNGT	3 cut(s)	975, 1476, 1750
TaiI	ACGT	3 cut(s)	1491, 1509, 1646
TaqI	TCGA	1 cut(s)	849
TatI	WGTACW	2 cut(s)	378, 1476
TauI	GCSGC	1 cut(s)	119
TfiI	GAWTC	1 cut(s)	477
Tru1I	TTAA	5 cut(s)	104, 569, 704, 825, 1271
Tru9I	TTAA	5 cut(s)	104, 569, 704, 825, 1271
TscAI	CASTG	2 cut(s)	291, 1659
TseFI	GTSAC	5 cut(s)	152, 334, 430, 927, 1650
TseI	GCWGC	7 cut(s)	587, 767, 1531, 1538, 1622, 1625, 1727
Tsp45I	GTSAC	5 cut(s)	152, 334, 430, 927, 1650
TspDTI	ATGAA	7 cut(s)	73, 307, 641, 663, 725, 1057, 1600
TspGWI	ACGGA	1 cut(s)	1339
TspRI	CASTG	2 cut(s)	291, 1659
VpaK11BI	GGWCC	2 cut(s)	14, 277
XagI	CCTNNNNNAGG	1 cut(s)	21
XapI	RAATTY	2 cut(s)	397, 821
XceI	RCATGY	3 cut(s)	136, 1057, 1077
XcmI	CCANNNNNNNNNTGG	1 cut(s)	68
XhoI	CTCGAG	1 cut(s)	848
XmnI	GAANNNNTTC	1 cut(s)	912
XspI	CTAG	5 cut(s)	53, 762, 1178, 1343, 1461
Zsp2I	ATGCAT	1 cut(s)	1079

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.