FvH4_7g19860

synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004698
Physical Location & Seq
Reverse (-)
16297110 .. 16303116
6007 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g19860.t1

Sequence Viewer

Length: 1128 bp
ATGTCAATGTCGACCTCATCGCTCTCAACTCAAACGTTCGGTTTGCCGTGGCCCGATCTCAACGACGGCTTGTCGTACAACGACGTCGTCAAATCCTCCGATTCCGAATTGACACTGATCGAGTTCTACTCCAGCAAGTACAAGAGCTCAGCTCCGTTGCAAGGTTGGTTGCAGAGAATCCGAAACGGACAGATAACAGTTGATGGGGAAGTTGTTAGAGATTCGGATACGATTCTCAGGGTTGGTTCGGAATTGGTATATCATAGACTTCCGTGGGGAGAGCCGGATGCGCCATACTTGCTTGAGGTTTTGTATGAGGATGATGATATGATTGCTTTGAATAAGCCGGGCGGGCTGCAAGTTTTACCTGGAGGTCTTTTCCAGCAACGGACAGTTCTAACACAGCTCCTATGGCGGGAGTCTCAGAAATTCGATCACTTAGCATGCCAAAAATCACATCTTGTTCCTGTTCATCGCCTAGGAAGGGGGACTTCAGGAATACTGCTATGTGCAAAGACGAAGCATGCCAAAACTCAACTTGCCGCATATTTTGCTGATGGGACATCCCACATTGGAGATGACAGCACCACCAACACGAAGCTGCATACAACCAGGAAAATCACAAAGATATACCGGGCACTAGTAACTGGGATAATTTGTGAGGATGAGGTGATTGTCAAGCAGCCAATTGGAACTGTGCGATATCCTGGTGTGGCTAAAGGGCTGTATATTGCTTCTCCGGCAGGAAAACCAGCTCTGAGCAAAGTAGAAGTTCTTGACAGGGACATACAGAAAAACCACACAATTGTACAGGTTGAGATTCAGTCAGGGAGACCACATCAAATTCGCATTCATCTGTCTTTCATAGGACATCCCTTGCTAGGAGATCCTCTATATGTTGCTGGTGGACAACCAAAGTGCTTGGGATCTGACTTAGTTGATGAAAGTTTTGCAGATGACGGAGGGTTCAAGAGGCCTACAAAGCCTGTTCCTGGAGATTGTGGTTATAATCTGCATGCTCATCAAGTGGTACTCTCTCACCCATCATCCAATGAGGTAATCAAAATCACAGCACCTCTCCCACTTGTCCTTCGGACTCGAGAAGAGATTGAAGAACTTAGGCAGTAG

Protein Analysis

376

Amino Acids

41.41

Weight (kDa)

6.7

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 110 - 287 8.5e-22 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1008
AatII GACGTC 1 cut(s) 87
AccI GTMKAC 1 cut(s) 11
AciI CCGC 3 cut(s) 351, 415, 543
AclI AACGTT 1 cut(s) 35
AclWI GGATC 2 cut(s) 881, 934
AcsI RAATTY 2 cut(s) 428, 843
AcuI CTGAAG 1 cut(s) 477
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 4 cut(s) 77, 140, 810, 1032
AfiI CCNNNNNNNGG 5 cut(s) 161, 415, 484, 881, 992
AgsI TTSAA 3 cut(s) 340, 970, 1112
AhdI GACNNNNNGTC 1 cut(s) 70
AhlI ACTAGT 1 cut(s) 640
AjnI CCWGG 4 cut(s) 367, 611, 706, 991
AluBI AGCT 5 cut(s) 147, 152, 406, 601, 755
AluI AGCT 5 cut(s) 147, 152, 406, 601, 755
Alw21I GWGCWC 1 cut(s) 149
Alw26I GTCTC 2 cut(s) 426, 826
AlwI GGATC 2 cut(s) 881, 934
Ama87I CYCGRG 1 cut(s) 1098
AoxI GGCC 2 cut(s) 50, 974
ApeKI GCWGC 3 cut(s) 355, 601, 682
ApoI RAATTY 2 cut(s) 428, 843
AspA2I CCTAGG 1 cut(s) 478
AspLEI GCGC 1 cut(s) 292
AspS9I GGNCC 1 cut(s) 51
AsuC2I CCSGG 2 cut(s) 348, 635
AsuHPI GGTGA 2 cut(s) 682, 1031
AvaI CYCGRG 1 cut(s) 1098
AvrII CCTAGG 1 cut(s) 478
BaeGI GKGCMC 1 cut(s) 640
BanII GRGCYC 1 cut(s) 149
Bbv12I GWGCWC 1 cut(s) 149
BbvI GCAGC 3 cut(s) 342, 588, 694
BccI CCATC 3 cut(s) 197, 551, 1051
BceAI ACGGC 2 cut(s) 31, 82
BciT130I CCWGG 4 cut(s) 369, 613, 708, 993
BciVI GTATCC 1 cut(s) 220
BcnI CCSGG 2 cut(s) 348, 635
BcoDI GTCTC 2 cut(s) 426, 826
BcuI ACTAGT 1 cut(s) 640
BfaI CTAG 3 cut(s) 479, 641, 881
BfuI GTATCC 1 cut(s) 220
BglI GCCNNNNNGGC 1 cut(s) 352
BisI GCNGC 4 cut(s) 356, 543, 602, 683
BlnI CCTAGG 1 cut(s) 478
BlpI GCTNAGC 1 cut(s) 148
BlsI GCNGC 4 cut(s) 357, 544, 603, 684
Bme1390I CCNGG 6 cut(s) 348, 369, 613, 635, 708, 993
BmeRI GACNNNNNGTC 1 cut(s) 70
BmeT110I CYCGRG 1 cut(s) 1098
BmgT120I GGNCC 1 cut(s) 51
BmrFI CCNGG 6 cut(s) 348, 369, 613, 635, 708, 993
BmrI ACTGGG 1 cut(s) 657
BmsI GCATC 1 cut(s) 277
BmuI ACTGGG 1 cut(s) 657
BplI GAGNNNNNCTC 4 cut(s) 113, 145, 136, 168
BpmI CTGGAG 3 cut(s) 115, 390, 1014
Bpu1102I GCTNAGC 1 cut(s) 148
BpuEI CTTGAG 1 cut(s) 323
BpuMI CCSGG 2 cut(s) 348, 635
BsaHI GRCGYC 1 cut(s) 84
BsaI GGTCTC 1 cut(s) 826
BsaJI CCNNGG 3 cut(s) 47, 272, 478
Bsc4I CCNNNNNNNGG 5 cut(s) 161, 415, 484, 881, 992
Bse1I ACTGG 1 cut(s) 652
BseBI CCWGG 4 cut(s) 369, 613, 708, 993
BseDI CCNNGG 3 cut(s) 47, 272, 478
BseGI GGATG 6 cut(s) 292, 325, 563, 670, 871, 1046
BseLI CCNNNNNNNGG 5 cut(s) 161, 415, 484, 881, 992
BseMII CTCAG 4 cut(s) 162, 250, 437, 749
BseNI ACTGG 1 cut(s) 652
BseSI GKGCMC 1 cut(s) 640
BseXI GCAGC 3 cut(s) 342, 588, 694
BshFI GGCC 2 cut(s) 52, 976
BsiHKAI GWGCWC 1 cut(s) 149
BsiHKCI CYCGRG 1 cut(s) 1098
BsiSI CCGG 4 cut(s) 284, 347, 634, 740
BslFI GGGAC 3 cut(s) 502, 574, 797
BslI CCNNNNNNNGG 5 cut(s) 161, 415, 484, 881, 992
BsmAI GTCTC 2 cut(s) 426, 826
BsmFI GGGAC 3 cut(s) 502, 574, 797
BsmI GAATGC 1 cut(s) 849
BsnI GGCC 2 cut(s) 52, 976
Bso31I GGTCTC 1 cut(s) 826
BsoBI CYCGRG 1 cut(s) 1098
Bsp1286I GDGCHC 2 cut(s) 149, 640
Bsp1407I TGTACA 1 cut(s) 808
Bsp143I GATC 5 cut(s) 55, 117, 433, 886, 926
Bsp1720I GCTNAGC 1 cut(s) 148
BspACI CCGC 3 cut(s) 351, 415, 543
BspANI GGCC 2 cut(s) 52, 976
BspCNI CTCAG 4 cut(s) 161, 249, 436, 750
BspPI GGATC 2 cut(s) 881, 934
BspTNI GGTCTC 1 cut(s) 826
BsrGI TGTACA 1 cut(s) 808
BsrI ACTGG 1 cut(s) 652
BssECI CCNNGG 3 cut(s) 47, 272, 478
BssMI GATC 5 cut(s) 55, 117, 433, 886, 926
BssNI GRCGYC 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 478
Bst2UI CCWGG 4 cut(s) 369, 613, 708, 993
Bst4CI ACNGT 3 cut(s) 199, 394, 697
Bst6I CTCTTC 1 cut(s) 1098
BstACI GRCGYC 1 cut(s) 84
BstAPI GCANNNNNTGC 1 cut(s) 551
BstAUI TGTACA 1 cut(s) 808
BstC8I GCNNGC 4 cut(s) 353, 445, 525, 1017
BstDEI CTNAG 7 cut(s) 148, 236, 423, 439, 758, 934, 1118
BstDSI CCRYGG 2 cut(s) 47, 272
BstF5I GGATG 6 cut(s) 292, 325, 563, 670, 871, 1046
BstHHI GCGC 1 cut(s) 292
BstKTI GATC 5 cut(s) 58, 120, 436, 889, 929
BstMAI GTCTC 2 cut(s) 426, 826
BstMBI GATC 5 cut(s) 55, 117, 433, 886, 926
BstMWI GCNNNNNNNGC 7 cut(s) 289, 298, 352, 412, 551, 740, 982
BstNI CCWGG 4 cut(s) 369, 613, 708, 993
BstNSI RCATGY 3 cut(s) 447, 527, 1019
BstSCI CCNGG 6 cut(s) 346, 367, 611, 633, 706, 991
BstSLI GKGCMC 1 cut(s) 640
BstV1I GCAGC 3 cut(s) 342, 588, 694
BstX2I RGATCY 2 cut(s) 886, 926
BstYI RGATCY 2 cut(s) 886, 926
BsuI GTATCC 1 cut(s) 220
BsuRI GGCC 2 cut(s) 52, 976
BtgI CCRYGG 2 cut(s) 47, 272
BtgZI GCGATG 2 cut(s) 3, 458
BtsCI GGATG 6 cut(s) 292, 325, 563, 670, 871, 1046
BtsIMutI CAGTG 1 cut(s) 113
Cac8I GCNNGC 4 cut(s) 353, 445, 525, 1017
CfoI GCGC 1 cut(s) 292
Cfr13I GGNCC 1 cut(s) 51
Csp6I GTAC 4 cut(s) 76, 139, 809, 1031
CviAII CATG 3 cut(s) 444, 524, 1016
CviQI GTAC 4 cut(s) 76, 139, 809, 1031
DdeI CTNAG 7 cut(s) 148, 236, 423, 439, 758, 934, 1118
DpnI GATC 5 cut(s) 57, 119, 435, 888, 928
DpnII GATC 5 cut(s) 55, 117, 433, 886, 926
DriI GACNNNNNGTC 1 cut(s) 70
Eam1104I CTCTTC 1 cut(s) 1098
Eam1105I GACNNNNNGTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 1098
Ecl136II GAGCTC 1 cut(s) 147
Eco130I CCWWGG 1 cut(s) 478
Eco147I AGGCCT 1 cut(s) 976
Eco24I GRGCYC 1 cut(s) 149
Eco31I GGTCTC 1 cut(s) 826
Eco32I GATATC 1 cut(s) 704
Eco53kI GAGCTC 1 cut(s) 147
Eco57I CTGAAG 1 cut(s) 477
Eco88I CYCGRG 1 cut(s) 1098
EcoICRI GAGCTC 1 cut(s) 147
EcoRII CCWGG 4 cut(s) 367, 611, 706, 991
EcoRV GATATC 1 cut(s) 704
EcoT14I CCWWGG 1 cut(s) 478
EcoT38I GRGCYC 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 478
FaeI CATG 3 cut(s) 447, 527, 1019
FalI AAGNNNNNCTT 2 cut(s) 475, 507
FaqI GGGAC 3 cut(s) 502, 574, 797
FatI CATG 3 cut(s) 443, 523, 1015
FauI CCCGC 2 cut(s) 344, 408
FblI GTMKAC 1 cut(s) 11
Fnu4HI GCNGC 4 cut(s) 356, 543, 602, 683
FokI GGATG 6 cut(s) 299, 332, 550, 677, 858, 1033
FriOI GRGCYC 1 cut(s) 149
Fsp4HI GCNGC 4 cut(s) 356, 543, 602, 683
FspBI CTAG 3 cut(s) 479, 641, 881
GlaI GCGC 1 cut(s) 291
GluI GCNGC 4 cut(s) 356, 543, 602, 683
GsuI CTGGAG 3 cut(s) 115, 390, 1014
HaeIII GGCC 2 cut(s) 52, 976
HapII CCGG 4 cut(s) 284, 347, 634, 740
HhaI GCGC 1 cut(s) 292
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 3 cut(s) 447, 527, 1019
Hin6I GCGC 1 cut(s) 290
HinP1I GCGC 1 cut(s) 290
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HinfI GANTC 7 cut(s) 101, 177, 221, 232, 419, 820, 1096
HpaII CCGG 4 cut(s) 284, 347, 634, 740
HphI GGTGA 2 cut(s) 682, 1031
Hpy166II GTNNAC 2 cut(s) 12, 908
Hpy188I TCNGA 9 cut(s) 100, 106, 182, 226, 250, 426, 759, 931, 1095
Hpy188III TCNNGA 4 cut(s) 495, 776, 970, 1100
Hpy8I GTNNAC 2 cut(s) 12, 908
Hpy99I CGWCG 3 cut(s) 68, 86, 89
HpyAV CCTTC 2 cut(s) 477, 1100
HpyCH4III ACNGT 3 cut(s) 199, 394, 697
HpyCH4IV ACGT 2 cut(s) 35, 84
HpyCH4V TGCA 7 cut(s) 160, 172, 358, 512, 604, 953, 1015
HpyF10VI GCNNNNNNNGC 7 cut(s) 289, 298, 352, 412, 551, 740, 982
HpyF3I CTNAG 7 cut(s) 148, 236, 423, 439, 758, 934, 1118
HpySE526I ACGT 2 cut(s) 35, 84
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 3 cut(s) 447, 527, 1019
HspAI GCGC 1 cut(s) 290
Kzo9I GATC 5 cut(s) 55, 117, 433, 886, 926
LmnI GCTCC 2 cut(s) 157, 411
Lsp1109I GCAGC 3 cut(s) 342, 588, 694
LweI GCATC 1 cut(s) 277
MaeI CTAG 3 cut(s) 479, 641, 881
MaeII ACGT 2 cut(s) 35, 84
MaeIII GTNAC 1 cut(s) 643
MalI GATC 5 cut(s) 57, 119, 435, 888, 928
MboI GATC 5 cut(s) 55, 117, 433, 886, 926
MboII GAAGA 2 cut(s) 1115, 1124
MfeI CAATTG 2 cut(s) 687, 804
MflI RGATCY 2 cut(s) 886, 926
MhlI GDGCHC 2 cut(s) 149, 640
MluCI AATT 7 cut(s) 107, 251, 428, 654, 687, 804, 843
MlyI GAGTC 2 cut(s) 428, 1090
MspI CCGG 4 cut(s) 284, 347, 634, 740
MspR9I CCNGG 6 cut(s) 348, 369, 613, 635, 708, 993
MunI CAATTG 2 cut(s) 687, 804
Mva1269I GAATGC 1 cut(s) 849
MvaI CCWGG 4 cut(s) 369, 613, 708, 993
MwoI GCNNNNNNNGC 7 cut(s) 289, 298, 352, 412, 551, 740, 982
NciI CCSGG 2 cut(s) 348, 635
NdeII GATC 5 cut(s) 55, 117, 433, 886, 926
NlaIII CATG 3 cut(s) 447, 527, 1019
NspI RCATGY 3 cut(s) 447, 527, 1019
PaeI GCATGC 3 cut(s) 447, 527, 1019
PaeR7I CTCGAG 1 cut(s) 1098
PceI AGGCCT 1 cut(s) 976
PctI GAATGC 1 cut(s) 849
PfeI GAWTC 5 cut(s) 101, 177, 221, 232, 820
PflFI GACNNNGTC 1 cut(s) 86
PfoI TCCNGGA 1 cut(s) 991
PkrI GCNGC 4 cut(s) 357, 544, 603, 684
PleI GAGTC 2 cut(s) 427, 1090
PpsI GAGTC 2 cut(s) 427, 1090
PsiI TTATAA 1 cut(s) 1008
Psp124BI GAGCTC 1 cut(s) 149
Psp1406I AACGTT 1 cut(s) 35
Psp6I CCWGG 4 cut(s) 367, 611, 706, 991
PspGI CCWGG 4 cut(s) 367, 611, 706, 991
PspPI GGNCC 1 cut(s) 51
PsuI RGATCY 2 cut(s) 886, 926
PsyI GACNNNGTC 1 cut(s) 86
RsaI GTAC 4 cut(s) 77, 140, 810, 1032
RsaNI GTAC 4 cut(s) 76, 139, 809, 1031
SacI GAGCTC 1 cut(s) 149
SalI GTCGAC 1 cut(s) 10
SatI GCNGC 4 cut(s) 356, 543, 602, 683
Sau3AI GATC 5 cut(s) 55, 117, 433, 886, 926
Sau96I GGNCC 1 cut(s) 51
SchI GAGTC 2 cut(s) 428, 1090
ScrFI CCNGG 6 cut(s) 348, 369, 613, 635, 708, 993
SduI GDGCHC 2 cut(s) 149, 640
SfaNI GCATC 1 cut(s) 277
Sfr274I CTCGAG 1 cut(s) 1098
SlaI CTCGAG 1 cut(s) 1098
SmlI CTYRAG 2 cut(s) 302, 1098
SmoI CTYRAG 2 cut(s) 302, 1098
SpeI ACTAGT 1 cut(s) 640
SphI GCATGC 3 cut(s) 447, 527, 1019
Sse9I AATT 7 cut(s) 107, 251, 428, 654, 687, 804, 843
SseBI AGGCCT 1 cut(s) 976
SsiI CCGC 3 cut(s) 351, 415, 543
SspMI CTAG 3 cut(s) 479, 641, 881
SstI GAGCTC 1 cut(s) 149
StuI AGGCCT 1 cut(s) 976
StyD4I CCNGG 6 cut(s) 346, 367, 611, 633, 706, 991
StyI CCWWGG 1 cut(s) 478
TaaI ACNGT 3 cut(s) 199, 394, 697
TaiI ACGT 2 cut(s) 38, 87
TaqI TCGA 4 cut(s) 11, 120, 432, 1099
TasI AATT 7 cut(s) 107, 251, 428, 654, 687, 804, 843
TatI WGTACW 2 cut(s) 138, 808
TauI GCSGC 1 cut(s) 545
TfiI GAWTC 5 cut(s) 101, 177, 221, 232, 820
TscAI CASTG 1 cut(s) 120
TseI GCWGC 3 cut(s) 355, 601, 682
TspDTI ATGAA 4 cut(s) 461, 842, 853, 957
TspGWI ACGGA 5 cut(s) 144, 201, 261, 403, 975
TspRI CASTG 1 cut(s) 120
Tth111I GACNNNGTC 1 cut(s) 86
XapI RAATTY 2 cut(s) 428, 843
XceI RCATGY 3 cut(s) 447, 527, 1019
XhoI CTCGAG 1 cut(s) 1098
XmaJI CCTAGG 1 cut(s) 478
XmiI GTMKAC 1 cut(s) 11
XspI CTAG 3 cut(s) 479, 641, 881
ZraI GACGTC 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.