RchiOBHm_Chr2g0110391

synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004698
Physical Location & Seq
Forward (+)
21864557 .. 21866638
2082 bp
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UTR
Exon/CDS
Intron
PRQ48407

Sequence Viewer

Length: 582 bp
ATGAACATCTGCTTTGGAAATTTACGGCATTGCTTTGTTGGTCTACAGATAACAGTTGATGGAGAAGTTATTAGAGACTTGAATATGATCCTCAGGGTTGGTTCACAATTGGTCTATCATAAGCTTCCGTGGAGAGAGCCGGATGCGCCATACTTGCTGGAGGTTCTGTATGAGGATGATGATATGCAACGGACAGTTCTAACACAGCTCTTATGGCGGCAAACTCAGAAGATCCATCACTTAGCATGCCAAAAATCACATCTTGTTCCTGTTCATCGCCTAGGAAGGGGCACTTCAGGAATACTGCTAGGTGCAAAGATAAATCATGCCAAAAAGCAACTTGCAGCATATTTTGCTGATGGGACATCCCATATTGGAGATGACAGGAAAATCTCAAAGATATATCGGGCCCTAGGGACTGGGATACTTTGTGAGGAGGAGGTAATGCTATCTCATGAGACTCTGAAGTTTGTGAGTGACTATGTCTTCATGTGCAATCTTAATGTTGGTATCTCAAAGACCATTTCTGTCCCATGTTTCTATAGCTTCTTTGATCTAGAAAGTTGCATACTTGGTTTTTAG

Protein Analysis

193

Amino Acids

21.97

Weight (kDa)

7.1

Isoelectric Point (pI)

33.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 84 - 140 1.8e-07 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 43
AciI CCGC 1 cut(s) 217
AclWI GGATC 2 cut(s) 82, 226
AcsI RAATTY 1 cut(s) 19
AcuI CTGAAG 2 cut(s) 279, 485
AfiI CCNNNNNNNGG 1 cut(s) 286
AgsI TTSAA 1 cut(s) 82
AluBI AGCT 3 cut(s) 124, 208, 546
AluI AGCT 3 cut(s) 124, 208, 546
Alw26I GTCTC 2 cut(s) 69, 452
AlwI GGATC 2 cut(s) 82, 226
AoxI GGCC 1 cut(s) 408
ApaI GGGCCC 1 cut(s) 412
ApeKI GCWGC 1 cut(s) 344
ApoI RAATTY 1 cut(s) 19
AspA2I CCTAGG 2 cut(s) 280, 412
AspLEI GCGC 1 cut(s) 148
AspS9I GGNCC 2 cut(s) 408, 409
AvrII CCTAGG 2 cut(s) 280, 412
AxyI CCTNAGG 1 cut(s) 92
BaeGI GKGCMC 2 cut(s) 293, 412
BanII GRGCYC 1 cut(s) 412
BbsI GAAGAC 1 cut(s) 478
BbvI GCAGC 1 cut(s) 356
BccI CCATC 3 cut(s) 53, 243, 353
BceAI ACGGC 1 cut(s) 41
BciVI GTATCC 1 cut(s) 417
BcoDI GTCTC 2 cut(s) 69, 452
BfaI CTAG 4 cut(s) 281, 308, 413, 557
BfmI CTRYAG 2 cut(s) 44, 541
BfuI GTATCC 1 cut(s) 417
BisI GCNGC 2 cut(s) 218, 345
BlnI CCTAGG 2 cut(s) 280, 412
BlsI GCNGC 2 cut(s) 219, 346
BmgT120I GGNCC 2 cut(s) 408, 409
BmiI GGNNCC 1 cut(s) 410
BmrI ACTGGG 1 cut(s) 429
BmsI GCATC 1 cut(s) 133
BmuI ACTGGG 1 cut(s) 429
BpiI GAAGAC 1 cut(s) 478
BpmI CTGGAG 1 cut(s) 179
BsaJI CCNNGG 3 cut(s) 128, 280, 412
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 1 cut(s) 286
Bse1I ACTGG 1 cut(s) 424
Bse21I CCTNAGG 1 cut(s) 92
Bse3DI GCAATG 1 cut(s) 28
BseDI CCNNGG 3 cut(s) 128, 280, 412
BseGI GGATG 3 cut(s) 148, 181, 365
BseLI CCNNNNNNNGG 1 cut(s) 286
BseMI GCAATG 1 cut(s) 28
BseMII CTCAG 2 cut(s) 106, 239
BseNI ACTGG 1 cut(s) 424
BseRI GAGGAG 2 cut(s) 449, 452
BseSI GKGCMC 2 cut(s) 293, 412
BseXI GCAGC 1 cut(s) 356
BshFI GGCC 1 cut(s) 410
BsiSI CCGG 1 cut(s) 140
BslFI GGGAC 3 cut(s) 376, 430, 515
BslI CCNNNNNNNGG 1 cut(s) 286
BsmAI GTCTC 2 cut(s) 69, 452
BsmFI GGGAC 3 cut(s) 376, 430, 515
BsnI GGCC 1 cut(s) 410
Bsp120I GGGCCC 1 cut(s) 408
Bsp1286I GDGCHC 2 cut(s) 293, 412
Bsp143I GATC 3 cut(s) 87, 231, 553
BspACI CCGC 1 cut(s) 217
BspANI GGCC 1 cut(s) 410
BspCNI CTCAG 2 cut(s) 105, 238
BspHI TCATGA 1 cut(s) 454
BspLI GGNNCC 1 cut(s) 410
BspPI GGATC 2 cut(s) 82, 226
BsrDI GCAATG 1 cut(s) 28
BsrI ACTGG 1 cut(s) 424
BssECI CCNNGG 3 cut(s) 128, 280, 412
BssMI GATC 3 cut(s) 87, 231, 553
BssT1I CCWWGG 2 cut(s) 280, 412
Bst4CI ACNGT 2 cut(s) 55, 196
BstAPI GCANNNNNTGC 1 cut(s) 353
BstC8I GCNNGC 1 cut(s) 247
BstDEI CTNAG 3 cut(s) 92, 225, 241
BstDSI CCRYGG 1 cut(s) 128
BstF5I GGATG 3 cut(s) 148, 181, 365
BstHHI GCGC 1 cut(s) 148
BstKTI GATC 3 cut(s) 90, 234, 556
BstMAI GTCTC 2 cut(s) 69, 452
BstMBI GATC 3 cut(s) 87, 231, 553
BstMWI GCNNNNNNNGC 4 cut(s) 145, 154, 214, 353
BstNSI RCATGY 1 cut(s) 249
BstSFI CTRYAG 2 cut(s) 44, 541
BstSLI GKGCMC 2 cut(s) 293, 412
BstV1I GCAGC 1 cut(s) 356
BstV2I GAAGAC 1 cut(s) 478
BstX2I RGATCY 1 cut(s) 231
BstYI RGATCY 1 cut(s) 231
Bsu36I CCTNAGG 1 cut(s) 92
BsuI GTATCC 1 cut(s) 417
BsuRI GGCC 1 cut(s) 410
BtgI CCRYGG 1 cut(s) 128
BtgZI GCGATG 1 cut(s) 260
BtsCI GGATG 3 cut(s) 148, 181, 365
Cac8I GCNNGC 1 cut(s) 247
CciI TCATGA 1 cut(s) 454
CfoI GCGC 1 cut(s) 148
Cfr13I GGNCC 2 cut(s) 408, 409
CviAII CATG 5 cut(s) 246, 326, 455, 490, 534
CviJI RGCY 5 cut(s) 124, 139, 208, 410, 546
CviKI_1 RGCY 5 cut(s) 124, 139, 208, 410, 546
DdeI CTNAG 3 cut(s) 92, 225, 241
DpnI GATC 3 cut(s) 89, 233, 555
DpnII GATC 3 cut(s) 87, 231, 553
Eco130I CCWWGG 2 cut(s) 280, 412
Eco24I GRGCYC 1 cut(s) 412
Eco57I CTGAAG 2 cut(s) 279, 485
Eco81I CCTNAGG 1 cut(s) 92
EcoO109I RGGNCCY 1 cut(s) 409
EcoT14I CCWWGG 2 cut(s) 280, 412
EcoT38I GRGCYC 1 cut(s) 412
ErhI CCWWGG 2 cut(s) 280, 412
FaeI CATG 5 cut(s) 249, 329, 458, 493, 537
FalI AAGNNNNNCTT 2 cut(s) 277, 309
FaqI GGGAC 3 cut(s) 376, 430, 515
FatI CATG 5 cut(s) 245, 325, 454, 489, 533
FblI GTMKAC 1 cut(s) 43
Fnu4HI GCNGC 2 cut(s) 218, 345
FokI GGATG 3 cut(s) 155, 188, 352
FriOI GRGCYC 1 cut(s) 412
Fsp4HI GCNGC 2 cut(s) 218, 345
FspBI CTAG 4 cut(s) 281, 308, 413, 557
GlaI GCGC 1 cut(s) 147
GluI GCNGC 2 cut(s) 218, 345
GsuI CTGGAG 1 cut(s) 179
HaeIII GGCC 1 cut(s) 410
HapII CCGG 1 cut(s) 140
HhaI GCGC 1 cut(s) 148
Hin1II CATG 5 cut(s) 249, 329, 458, 493, 537
Hin6I GCGC 1 cut(s) 146
HinP1I GCGC 1 cut(s) 146
HindIII AAGCTT 1 cut(s) 122
HinfI GANTC 1 cut(s) 460
HpaII CCGG 1 cut(s) 140
Hpy166II GTNNAC 2 cut(s) 44, 104
Hpy188I TCNGA 2 cut(s) 228, 465
Hpy188III TCNNGA 3 cut(s) 297, 455, 557
Hpy8I GTNNAC 2 cut(s) 44, 104
HpyAV CCTTC 1 cut(s) 279
HpyCH4III ACNGT 2 cut(s) 55, 196
HpyCH4V TGCA 5 cut(s) 187, 314, 344, 495, 567
HpyF10VI GCNNNNNNNGC 4 cut(s) 145, 154, 214, 353
HpyF3I CTNAG 3 cut(s) 92, 225, 241
Hsp92II CATG 5 cut(s) 249, 329, 458, 493, 537
HspAI GCGC 1 cut(s) 146
Kzo9I GATC 3 cut(s) 87, 231, 553
LpnPI CCDG 7 cut(s) 79, 143, 153, 282, 282, 370, 405
Lsp1109I GCAGC 1 cut(s) 356
LweI GCATC 1 cut(s) 133
MaeI CTAG 4 cut(s) 281, 308, 413, 557
MaeIII GTNAC 1 cut(s) 476
MalI GATC 3 cut(s) 89, 233, 555
MboI GATC 3 cut(s) 87, 231, 553
MboII GAAGA 2 cut(s) 241, 478
MfeI CAATTG 1 cut(s) 107
MflI RGATCY 1 cut(s) 231
MhlI GDGCHC 2 cut(s) 293, 412
MluCI AATT 2 cut(s) 19, 107
MlyI GAGTC 1 cut(s) 454
MnlI CCTC 6 cut(s) 101, 154, 166, 427, 430, 433
MseI TTAA 1 cut(s) 501
MspI CCGG 1 cut(s) 140
MunI CAATTG 1 cut(s) 107
MwoI GCNNNNNNNGC 4 cut(s) 145, 154, 214, 353
NdeII GATC 3 cut(s) 87, 231, 553
NlaIII CATG 5 cut(s) 249, 329, 458, 493, 537
NlaIV GGNNCC 1 cut(s) 410
NmuCI GTSAC 1 cut(s) 476
NspI RCATGY 1 cut(s) 249
PaeI GCATGC 1 cut(s) 249
PagI TCATGA 1 cut(s) 454
PflFI GACNNNGTC 1 cut(s) 482
PkrI GCNGC 2 cut(s) 219, 346
PleI GAGTC 1 cut(s) 454
PpsI GAGTC 1 cut(s) 454
PspN4I GGNNCC 1 cut(s) 410
PspOMI GGGCCC 1 cut(s) 408
PspPI GGNCC 2 cut(s) 408, 409
PsuI RGATCY 1 cut(s) 231
PsyI GACNNNGTC 1 cut(s) 482
SaqAI TTAA 1 cut(s) 501
SatI GCNGC 2 cut(s) 218, 345
Sau3AI GATC 3 cut(s) 87, 231, 553
Sau96I GGNCC 2 cut(s) 408, 409
SchI GAGTC 1 cut(s) 454
SduI GDGCHC 2 cut(s) 293, 412
SetI ASST 6 cut(s) 126, 165, 210, 313, 444, 548
SfaNI GCATC 1 cut(s) 133
SfcI CTRYAG 2 cut(s) 44, 541
SphI GCATGC 1 cut(s) 249
Sse9I AATT 2 cut(s) 19, 107
SsiI CCGC 1 cut(s) 217
SspMI CTAG 4 cut(s) 281, 308, 413, 557
StyI CCWWGG 2 cut(s) 280, 412
TaaI ACNGT 2 cut(s) 55, 196
TasI AATT 2 cut(s) 19, 107
TauI GCSGC 1 cut(s) 220
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TseFI GTSAC 1 cut(s) 476
TseI GCWGC 1 cut(s) 344
Tsp45I GTSAC 1 cut(s) 476
TspDTI ATGAA 3 cut(s) 17, 263, 478
TspGWI ACGGA 2 cut(s) 117, 205
Tth111I GACNNNGTC 1 cut(s) 482
XapI RAATTY 1 cut(s) 19
XbaI TCTAGA 1 cut(s) 556
XceI RCATGY 1 cut(s) 249
XmaJI CCTAGG 2 cut(s) 280, 412
XmiI GTMKAC 1 cut(s) 43
XspI CTAG 4 cut(s) 281, 308, 413, 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.