Rh1DG312800

synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0004698
Physical Location & Seq
Reverse (-)
53999431 .. 54004183
4753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG312800.1

Sequence Viewer

Length: 1218 bp
ATGTCGACCTCTTCGCTATCACCTCAAACTTTCGGATTGCCGTGGCCCGATCTCAACGACGGCTTGTCATACAACGACGTCGTTAAACCCTCCGATTCCGGTACTTCTCTCTTTCTCTCTTCTTCTTCTTCTCTGCGTATTCCGAACGTAGCTGAATCGCTTAACATCGACGCTTTGAACTTGTGTGAATTTACGGAATTGACACTGATCGAGTTCTACTCTAGCAAATACAAGAGCTCAGCTCCGTTGCAAGGTTGGTTGCAGAGAATCCGAAACGGACAGATAACAGTTGATGGAGAAGTTGTTAGAGATTCGAATACGATTCTCAGGGTTGGTTCGGAATTGGTCTATCATAGGCTTGCATGGAGAGAGCCGGATGCGCCATACTTGCTCGAGGTTCTATATGAGGATGATGATATGATTGCTTTGAATAAGCCAGGCGGGCTGCAAGTTTTACCTGGAGGTCTTTTTCAGCAACGGACAGTTCTAACACAGCTCCTATGGCGGCAAACTCAGACTCACTTAGCATGCCAAGAATCACATCCGGTTCCTGTTCATCGCCTAGGAAGGGGCACTTCAGGAATACTGCTATGTGCAAAGACGAAGCATGCCAAAACTCAACTTGCAGCATATTTTGCTGATGGGACATCCCATATTGGAGATGACAGTGACACCAACATGAAGCTGCATACAATAAGGAAAATCTCAAAGATATACCGGGCGCTAGTGACCGGGATACTTTGTGAGGACGAGGTGATTGTTAAGCAACCAATTGGAACTGTGCGATATCCTGGTGTGGCTAAAGGGCTGTATGTTGCTTCTCCCACAGGAAAACCAGCTCTGAGCAGAGTAGAAGTTCTTGAGAGGGACATACAGAAAAATCACACAATCGTACAGGTAGAGATTCAGTCAGGGAGACCACATCAAATACGCATTCATCTTTCTTTCATAGGACATCCTTTGCTAGGAGATCCTCTATATGTTGCTGGTGGACAACCAAAGTGCTTGGGATCTGACTTTGTGGATGAAAGTTTTGCAGATGATGGAGGGTTCAAGAGGCCTACGAAGCCTGTTCCTGGAGATTGTGGTTATAACTTGCATGCTCATCAAGTGGTTCTCTCTCACCCATCATCCAATGAGGGAATCAAAATTACAGCACCTCTCCCACTTGTCCTCCGGACTCGAGAAGAAATCGAAGAACTTAGTAGCGTTAGGTGA

Protein Analysis

405

Amino Acids

44.6

Weight (kDa)

6.4

Isoelectric Point (pI)

42.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 140 - 315 3.1e-22 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1092
AatII GACGTC 1 cut(s) 81
AccI GTMKAC 1 cut(s) 5
AccIII TCCGGA 1 cut(s) 1176
AciI CCGC 2 cut(s) 441, 505
AclWI GGATC 2 cut(s) 965, 1018
AcsI RAATTY 1 cut(s) 188
AcuI CTGAAG 1 cut(s) 561
AcyI GRCGYC 1 cut(s) 78
AfaI GTAC 2 cut(s) 103, 894
AfiI CCNNNNNNNGG 4 cut(s) 251, 568, 965, 1076
AgsI TTSAA 3 cut(s) 178, 430, 1054
AhdI GACNNNNNGTC 1 cut(s) 64
AjnI CCWGG 4 cut(s) 436, 457, 790, 1075
AluBI AGCT 6 cut(s) 152, 237, 242, 496, 685, 839
AluI AGCT 6 cut(s) 152, 237, 242, 496, 685, 839
Alw21I GWGCWC 1 cut(s) 239
Alw26I GTCTC 1 cut(s) 910
AlwI GGATC 2 cut(s) 965, 1018
Ama87I CYCGRG 2 cut(s) 392, 1182
Aor13HI TCCGGA 1 cut(s) 1176
AoxI GGCC 2 cut(s) 44, 1058
ApeKI GCWGC 3 cut(s) 445, 626, 685
ApoI RAATTY 1 cut(s) 188
AspA2I CCTAGG 1 cut(s) 562
AspLEI GCGC 2 cut(s) 382, 724
AspS9I GGNCC 1 cut(s) 45
AsuC2I CCSGG 2 cut(s) 719, 733
AsuHPI GGTGA 3 cut(s) 12, 766, 1115
AsuII TTCGAA 1 cut(s) 314
AvaI CYCGRG 2 cut(s) 392, 1182
AvrII CCTAGG 1 cut(s) 562
BaeGI GKGCMC 1 cut(s) 575
BanII GRGCYC 1 cut(s) 239
Bbv12I GWGCWC 1 cut(s) 239
BbvI GCAGC 3 cut(s) 432, 638, 672
BccI CCATC 4 cut(s) 287, 635, 1037, 1135
BceAI ACGGC 2 cut(s) 25, 76
BciT130I CCWGG 4 cut(s) 438, 459, 792, 1077
BciVI GTATCC 1 cut(s) 729
BcnI CCSGG 2 cut(s) 719, 733
BcoDI GTCTC 1 cut(s) 910
BfaI CTAG 4 cut(s) 222, 563, 725, 965
BfoI RGCGCY 1 cut(s) 725
BfuI GTATCC 1 cut(s) 729
BglI GCCNNNNNGGC 1 cut(s) 442
BisI GCNGC 4 cut(s) 446, 506, 627, 686
BlnI CCTAGG 1 cut(s) 562
BlpI GCTNAGC 1 cut(s) 238
BlsI GCNGC 4 cut(s) 447, 507, 628, 687
Bme1390I CCNGG 6 cut(s) 438, 459, 719, 733, 792, 1077
BmeRI GACNNNNNGTC 1 cut(s) 64
BmeT110I CYCGRG 2 cut(s) 392, 1182
BmgT120I GGNCC 1 cut(s) 45
BmiI GGNNCC 1 cut(s) 549
BmrFI CCNGG 6 cut(s) 438, 459, 719, 733, 792, 1077
BmsI GCATC 1 cut(s) 367
BplI GAGNNNNNCTC 4 cut(s) 203, 235, 226, 258
BpmI CTGGAG 2 cut(s) 480, 1098
Bpu1102I GCTNAGC 1 cut(s) 238
Bpu14I TTCGAA 1 cut(s) 314
BpuEI CTTGAG 1 cut(s) 881
BpuMI CCSGG 2 cut(s) 719, 733
BsaHI GRCGYC 1 cut(s) 78
BsaI GGTCTC 1 cut(s) 910
BsaJI CCNNGG 2 cut(s) 41, 562
BsaWI WCCGGW 3 cut(s) 98, 544, 1176
BsaXI ACNNNNNCTCC 4 cut(s) 288, 318, 1158, 1188
Bsc4I CCNNNNNNNGG 4 cut(s) 251, 568, 965, 1076
BseAI TCCGGA 1 cut(s) 1176
BseBI CCWGG 4 cut(s) 438, 459, 792, 1077
BseDI CCNNGG 2 cut(s) 41, 562
BseGI GGATG 7 cut(s) 382, 415, 541, 647, 955, 1030, 1130
BseLI CCNNNNNNNGG 4 cut(s) 251, 568, 965, 1076
BseMII CTCAG 4 cut(s) 252, 340, 527, 833
BseSI GKGCMC 1 cut(s) 575
BseXI GCAGC 3 cut(s) 432, 638, 672
BshFI GGCC 2 cut(s) 46, 1060
BsiHKAI GWGCWC 1 cut(s) 239
BsiHKCI CYCGRG 2 cut(s) 392, 1182
BsiSI CCGG 6 cut(s) 99, 374, 545, 718, 732, 1177
BslFI GGGAC 2 cut(s) 658, 881
BslI CCNNNNNNNGG 4 cut(s) 251, 568, 965, 1076
BsmAI GTCTC 1 cut(s) 910
BsmFI GGGAC 2 cut(s) 658, 881
BsmI GAATGC 1 cut(s) 933
BsnI GGCC 2 cut(s) 46, 1060
Bso31I GGTCTC 1 cut(s) 910
BsoBI CYCGRG 2 cut(s) 392, 1182
Bsp119I TTCGAA 1 cut(s) 314
Bsp1286I GDGCHC 2 cut(s) 239, 575
Bsp13I TCCGGA 1 cut(s) 1176
Bsp143I GATC 4 cut(s) 49, 207, 970, 1010
Bsp1720I GCTNAGC 1 cut(s) 238
BspACI CCGC 2 cut(s) 441, 505
BspANI GGCC 2 cut(s) 46, 1060
BspCNI CTCAG 4 cut(s) 251, 339, 526, 834
BspEI TCCGGA 1 cut(s) 1176
BspLI GGNNCC 1 cut(s) 549
BspPI GGATC 2 cut(s) 965, 1018
BspT104I TTCGAA 1 cut(s) 314
BspTNI GGTCTC 1 cut(s) 910
BssECI CCNNGG 2 cut(s) 41, 562
BssMI GATC 4 cut(s) 49, 207, 970, 1010
BssNI GRCGYC 1 cut(s) 78
BssT1I CCWWGG 1 cut(s) 562
Bst2UI CCWGG 4 cut(s) 438, 459, 792, 1077
Bst4CI ACNGT 4 cut(s) 289, 484, 668, 781
Bst6I CTCTTC 2 cut(s) 16, 124
BstACI GRCGYC 1 cut(s) 78
BstAPI GCANNNNNTGC 1 cut(s) 635
BstBI TTCGAA 1 cut(s) 314
BstC8I GCNNGC 5 cut(s) 360, 443, 529, 609, 1101
BstDEI CTNAG 6 cut(s) 238, 326, 513, 523, 842, 1202
BstDSI CCRYGG 1 cut(s) 41
BstENI CCTNNNNNAGG 1 cut(s) 963
BstF5I GGATG 7 cut(s) 382, 415, 541, 647, 955, 1030, 1130
BstH2I RGCGCY 1 cut(s) 725
BstHHI GCGC 2 cut(s) 382, 724
BstKTI GATC 4 cut(s) 52, 210, 973, 1013
BstMAI GTCTC 1 cut(s) 910
BstMBI GATC 4 cut(s) 49, 207, 970, 1010
BstMWI GCNNNNNNNGC 6 cut(s) 379, 388, 442, 502, 635, 1066
BstNI CCWGG 4 cut(s) 438, 459, 792, 1077
BstNSI RCATGY 3 cut(s) 531, 611, 1103
BstSCI CCNGG 6 cut(s) 436, 457, 717, 731, 790, 1075
BstSLI GKGCMC 1 cut(s) 575
BstV1I GCAGC 3 cut(s) 432, 638, 672
BstX2I RGATCY 2 cut(s) 970, 1010
BstYI RGATCY 2 cut(s) 970, 1010
BsuI GTATCC 1 cut(s) 729
BsuRI GGCC 2 cut(s) 46, 1060
BtgI CCRYGG 1 cut(s) 41
BtgZI GCGATG 1 cut(s) 542
BtsCI GGATG 7 cut(s) 382, 415, 541, 647, 955, 1030, 1130
BtsIMutI CAGTG 2 cut(s) 203, 673
Cac8I GCNNGC 5 cut(s) 360, 443, 529, 609, 1101
CfoI GCGC 2 cut(s) 382, 724
Cfr13I GGNCC 1 cut(s) 45
CseI GACGC 1 cut(s) 179
Csp6I GTAC 2 cut(s) 102, 893
CviAII CATG 5 cut(s) 363, 528, 608, 679, 1100
CviQI GTAC 2 cut(s) 102, 893
DdeI CTNAG 6 cut(s) 238, 326, 513, 523, 842, 1202
DpnI GATC 4 cut(s) 51, 209, 972, 1012
DpnII GATC 4 cut(s) 49, 207, 970, 1010
DriI GACNNNNNGTC 1 cut(s) 64
Eam1104I CTCTTC 2 cut(s) 16, 124
Eam1105I GACNNNNNGTC 1 cut(s) 64
EarI CTCTTC 2 cut(s) 16, 124
Ecl136II GAGCTC 1 cut(s) 237
Eco130I CCWWGG 1 cut(s) 562
Eco147I AGGCCT 1 cut(s) 1060
Eco24I GRGCYC 1 cut(s) 239
Eco31I GGTCTC 1 cut(s) 910
Eco32I GATATC 1 cut(s) 788
Eco53kI GAGCTC 1 cut(s) 237
Eco57I CTGAAG 1 cut(s) 561
Eco88I CYCGRG 2 cut(s) 392, 1182
EcoICRI GAGCTC 1 cut(s) 237
EcoNI CCTNNNNNAGG 1 cut(s) 963
EcoRII CCWGG 4 cut(s) 436, 457, 790, 1075
EcoRV GATATC 1 cut(s) 788
EcoT14I CCWWGG 1 cut(s) 562
EcoT38I GRGCYC 1 cut(s) 239
ErhI CCWWGG 1 cut(s) 562
FaeI CATG 5 cut(s) 366, 531, 611, 682, 1103
FalI AAGNNNNNCTT 2 cut(s) 559, 591
FaqI GGGAC 2 cut(s) 658, 881
FatI CATG 5 cut(s) 362, 527, 607, 678, 1099
FauI CCCGC 1 cut(s) 434
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 446, 506, 627, 686
FokI GGATG 7 cut(s) 389, 422, 528, 634, 942, 1037, 1117
FriOI GRGCYC 1 cut(s) 239
Fsp4HI GCNGC 4 cut(s) 446, 506, 627, 686
FspBI CTAG 4 cut(s) 222, 563, 725, 965
GlaI GCGC 2 cut(s) 381, 723
GluI GCNGC 4 cut(s) 446, 506, 627, 686
GsuI CTGGAG 2 cut(s) 480, 1098
HaeII RGCGCY 1 cut(s) 725
HaeIII GGCC 2 cut(s) 46, 1060
HapII CCGG 6 cut(s) 99, 374, 545, 718, 732, 1177
HgaI GACGC 1 cut(s) 179
HhaI GCGC 2 cut(s) 382, 724
Hin1I GRCGYC 1 cut(s) 78
Hin1II CATG 5 cut(s) 366, 531, 611, 682, 1103
Hin6I GCGC 2 cut(s) 380, 722
HinP1I GCGC 2 cut(s) 380, 722
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HpaII CCGG 6 cut(s) 99, 374, 545, 718, 732, 1177
HphI GGTGA 3 cut(s) 12, 766, 1115
Hpy166II GTNNAC 2 cut(s) 6, 992
Hpy188I TCNGA 8 cut(s) 35, 94, 144, 272, 340, 516, 843, 1015
Hpy188III TCNNGA 5 cut(s) 579, 860, 1054, 1177, 1184
Hpy8I GTNNAC 2 cut(s) 6, 992
Hpy99I CGWCG 4 cut(s) 62, 80, 83, 173
HpyAV CCTTC 1 cut(s) 561
HpyCH4III ACNGT 4 cut(s) 289, 484, 668, 781
HpyCH4IV ACGT 2 cut(s) 78, 147
HpyCH4V TGCA 9 cut(s) 250, 262, 362, 448, 596, 626, 688, 1037, 1099
HpyF10VI GCNNNNNNNGC 6 cut(s) 379, 388, 442, 502, 635, 1066
HpyF3I CTNAG 6 cut(s) 238, 326, 513, 523, 842, 1202
HpySE526I ACGT 2 cut(s) 78, 147
Hsp92I GRCGYC 1 cut(s) 78
Hsp92II CATG 5 cut(s) 366, 531, 611, 682, 1103
HspAI GCGC 2 cut(s) 380, 722
Kpn2I TCCGGA 1 cut(s) 1176
Kzo9I GATC 4 cut(s) 49, 207, 970, 1010
LmnI GCTCC 2 cut(s) 247, 501
Lsp1109I GCAGC 3 cut(s) 432, 638, 672
LweI GCATC 1 cut(s) 367
MaeI CTAG 4 cut(s) 222, 563, 725, 965
MaeII ACGT 2 cut(s) 78, 147
MaeIII GTNAC 2 cut(s) 668, 727
MalI GATC 4 cut(s) 51, 209, 972, 1012
MboI GATC 4 cut(s) 49, 207, 970, 1010
MboII GAAGA 7 cut(s) 3, 111, 114, 117, 120, 1199, 1208
MfeI CAATTG 1 cut(s) 771
MflI RGATCY 2 cut(s) 970, 1010
MhlI GDGCHC 2 cut(s) 239, 575
MluCI AATT 5 cut(s) 188, 197, 341, 771, 1149
MlyI GAGTC 2 cut(s) 511, 1174
MroI TCCGGA 1 cut(s) 1176
MseI TTAA 3 cut(s) 84, 162, 762
MslI CAYNNNNRTG 1 cut(s) 677
MspI CCGG 6 cut(s) 99, 374, 545, 718, 732, 1177
MspR9I CCNGG 6 cut(s) 438, 459, 719, 733, 792, 1077
MunI CAATTG 1 cut(s) 771
Mva1269I GAATGC 1 cut(s) 933
MvaI CCWGG 4 cut(s) 438, 459, 792, 1077
MwoI GCNNNNNNNGC 6 cut(s) 379, 388, 442, 502, 635, 1066
NciI CCSGG 2 cut(s) 719, 733
NdeII GATC 4 cut(s) 49, 207, 970, 1010
NlaIII CATG 5 cut(s) 366, 531, 611, 682, 1103
NlaIV GGNNCC 1 cut(s) 549
NmuCI GTSAC 2 cut(s) 668, 727
NspI RCATGY 3 cut(s) 531, 611, 1103
NspV TTCGAA 1 cut(s) 314
PaeI GCATGC 3 cut(s) 531, 611, 1103
PaeR7I CTCGAG 2 cut(s) 392, 1182
PceI AGGCCT 1 cut(s) 1060
PctI GAATGC 1 cut(s) 933
PfeI GAWTC 8 cut(s) 95, 155, 267, 311, 322, 536, 904, 1143
PfoI TCCNGGA 1 cut(s) 1075
PkrI GCNGC 4 cut(s) 447, 507, 628, 687
PleI GAGTC 2 cut(s) 511, 1174
PpsI GAGTC 2 cut(s) 511, 1174
PsiI TTATAA 1 cut(s) 1092
Psp124BI GAGCTC 1 cut(s) 239
Psp6I CCWGG 4 cut(s) 436, 457, 790, 1075
PspGI CCWGG 4 cut(s) 436, 457, 790, 1075
PspN4I GGNNCC 1 cut(s) 549
PspPI GGNCC 1 cut(s) 45
PspXI VCTCGAGB 1 cut(s) 392
PsuI RGATCY 2 cut(s) 970, 1010
RsaI GTAC 2 cut(s) 103, 894
RsaNI GTAC 2 cut(s) 102, 893
RseI CAYNNNNRTG 1 cut(s) 677
SacI GAGCTC 1 cut(s) 239
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 3 cut(s) 84, 162, 762
SatI GCNGC 4 cut(s) 446, 506, 627, 686
Sau3AI GATC 4 cut(s) 49, 207, 970, 1010
Sau96I GGNCC 1 cut(s) 45
SchI GAGTC 2 cut(s) 511, 1174
ScrFI CCNGG 6 cut(s) 438, 459, 719, 733, 792, 1077
SduI GDGCHC 2 cut(s) 239, 575
SfaNI GCATC 1 cut(s) 367
Sfr274I CTCGAG 2 cut(s) 392, 1182
SfuI TTCGAA 1 cut(s) 314
SlaI CTCGAG 2 cut(s) 392, 1182
SmiMI CAYNNNNRTG 1 cut(s) 677
SmlI CTYRAG 3 cut(s) 392, 860, 1182
SmoI CTYRAG 3 cut(s) 392, 860, 1182
SphI GCATGC 3 cut(s) 531, 611, 1103
Sse9I AATT 5 cut(s) 188, 197, 341, 771, 1149
SseBI AGGCCT 1 cut(s) 1060
SsiI CCGC 2 cut(s) 441, 505
SspMI CTAG 4 cut(s) 222, 563, 725, 965
SstI GAGCTC 1 cut(s) 239
StuI AGGCCT 1 cut(s) 1060
StyD4I CCNGG 6 cut(s) 436, 457, 717, 731, 790, 1075
StyI CCWWGG 1 cut(s) 562
TaaI ACNGT 4 cut(s) 289, 484, 668, 781
TaiI ACGT 2 cut(s) 81, 150
TaqI TCGA 7 cut(s) 5, 168, 210, 314, 393, 1183, 1194
TasI AATT 5 cut(s) 188, 197, 341, 771, 1149
TauI GCSGC 1 cut(s) 508
TfiI GAWTC 8 cut(s) 95, 155, 267, 311, 322, 536, 904, 1143
Tru1I TTAA 3 cut(s) 84, 162, 762
Tru9I TTAA 3 cut(s) 84, 162, 762
TscAI CASTG 2 cut(s) 210, 673
TseFI GTSAC 2 cut(s) 668, 727
TseI GCWGC 3 cut(s) 445, 626, 685
Tsp45I GTSAC 2 cut(s) 668, 727
TspDTI ATGAA 5 cut(s) 545, 695, 926, 937, 1041
TspGWI ACGGA 4 cut(s) 209, 234, 291, 493
TspRI CASTG 2 cut(s) 210, 673
XagI CCTNNNNNAGG 1 cut(s) 963
XapI RAATTY 1 cut(s) 188
XceI RCATGY 3 cut(s) 531, 611, 1103
XhoI CTCGAG 2 cut(s) 392, 1182
XmaJI CCTAGG 1 cut(s) 562
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 4 cut(s) 222, 563, 725, 965
ZraI GACGTC 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.