Rw5G039760

synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004698
Physical Location & Seq
Forward (+)
70284275 .. 70293000
8726 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G039760.1

Sequence Viewer

Length: 870 bp
ATGCCACTCCCTTCATCGTTAACCCAGATCTGGATTGCATCACAGCCACAAACCCGAGCACCATCCTCAGCCCCCCCCGATCCGTGCAGAAACCACCTTCGACGGAAACTTCCCACATGGGTTCGCCGAGTGGGTCGAGGCCCACGGCTTGATGGAGAAGTTATTAGAGACTCAAATATGATCCTCAGGGTTGGTTCACAATTGGTCTATCATAGGCTTCCGTGGAGAGAGCCGGATGCGCCATACTTGCTGGAGATTGCTTTGAATAAGCCAGGCAGGCTACAAGTTTTACCTGGAGGTCTTTTCCAGCAACGGACAGTTCTAACACAACTCCTATGGCGGCAAACTCAAAAGATCCATCACTTAGCATGCCAAAAATCACATCTTGTTCCTGTTCATCGCCTAGGAAGGGGCACTTCAGGAATACTGCTATGTGCAAAGATAAATCATGCCAAAAAGCAACTTGCAGCATATTTTGCTGATGGGACATCCCATATTGGAGATGACAGGAAAATCTCAAAGATATACAGGGCCCTAGGGACTGGGATACTTTGTGAGGAGGAGGTAATACTATCTCATGAGACTCTGAAGCAAGTGATTGGCATCTTGAAGGAGCTAGGAGCATTTCATATATTCTACAGCCAAGCCACAGCAGGAGAAGAAAAAACAAATGTTCAAGCTCTAGATCCAGTGGCCATTTCTATGTATGAGATGATGTCAAAACTCATTGTATCCAAAAATTTCTGTATGGTCTCAAACGGTCACCTCGTGCTTGGTTTGGAAGATTTTCACAGTTCATGCGGAAAGTTGGTTACAGGTAGAGCAACTCAGACCATACCTTGTTTCTCAAGCATCAACAAGGGAAGGTAA

Protein Analysis

289

Amino Acids

32.25

Weight (kDa)

9.82

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 86 - 188 6.5e-09 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 340, 801
AclWI GGATC 4 cut(s) 74, 175, 349, 680
AcoI YGGCCR 1 cut(s) 693
AcsI RAATTY 1 cut(s) 739
AcuI CTGAAG 2 cut(s) 402, 608
AdeI CACNNNGTG 1 cut(s) 769
AfiI CCNNNNNNNGG 2 cut(s) 30, 409
AgsI TTSAA 3 cut(s) 265, 610, 677
AjnI CCWGG 2 cut(s) 271, 292
AluBI AGCT 2 cut(s) 616, 680
AluI AGCT 2 cut(s) 616, 680
Alw21I GWGCWC 1 cut(s) 61
Alw26I GTCTC 3 cut(s) 162, 575, 757
AlwI GGATC 4 cut(s) 74, 175, 349, 680
Ama87I CYCGRG 1 cut(s) 54
AoxI GGCC 3 cut(s) 139, 531, 693
ApaI GGGCCC 1 cut(s) 535
ApeKI GCWGC 1 cut(s) 467
ApoI RAATTY 1 cut(s) 739
Asp700I GAANNNNTTC 1 cut(s) 786
AspA2I CCTAGG 2 cut(s) 403, 535
AspLEI GCGC 1 cut(s) 241
AspS9I GGNCC 3 cut(s) 140, 531, 532
AsuHPI GGTGA 1 cut(s) 755
AvaI CYCGRG 1 cut(s) 54
AvrII CCTAGG 2 cut(s) 403, 535
AxyI CCTNAGG 1 cut(s) 185
BaeGI GKGCMC 2 cut(s) 416, 535
BalI TGGCCA 1 cut(s) 695
BanII GRGCYC 1 cut(s) 535
BauI CACGAG 1 cut(s) 767
Bbv12I GWGCWC 1 cut(s) 61
BbvCI CCTCAGC 1 cut(s) 67
BbvI GCAGC 1 cut(s) 479
BccI CCATC 4 cut(s) 70, 146, 366, 476
BceAI ACGGC 1 cut(s) 161
BciT130I CCWGG 2 cut(s) 273, 294
BciVI GTATCC 2 cut(s) 540, 742
BcoDI GTCTC 3 cut(s) 162, 575, 757
BfaI CTAG 4 cut(s) 404, 536, 617, 683
BfmI CTRYAG 1 cut(s) 637
BfuI GTATCC 2 cut(s) 540, 742
BglI GCCNNNNNGGC 1 cut(s) 277
BglII AGATCT 1 cut(s) 27
BisI GCNGC 2 cut(s) 341, 468
BlnI CCTAGG 2 cut(s) 403, 535
BlsI GCNGC 2 cut(s) 342, 469
Bme1390I CCNGG 2 cut(s) 273, 294
BmeT110I CYCGRG 1 cut(s) 54
BmgT120I GGNCC 3 cut(s) 140, 531, 532
BmiI GGNNCC 1 cut(s) 533
BmrFI CCNGG 2 cut(s) 273, 294
BmrI ACTGGG 1 cut(s) 552
BmsI GCATC 4 cut(s) 47, 226, 612, 861
BmuI ACTGGG 1 cut(s) 552
BpmI CTGGAG 2 cut(s) 272, 315
Bpu10I CCTNAGC 1 cut(s) 67
BpuEI CTTGAG 1 cut(s) 832
BsaBI GATNNNNATC 1 cut(s) 602
BsaI GGTCTC 1 cut(s) 757
BsaJI CCNNGG 4 cut(s) 143, 221, 403, 535
Bsc4I CCNNNNNNNGG 2 cut(s) 30, 409
Bse1I ACTGG 2 cut(s) 547, 689
Bse21I CCTNAGG 1 cut(s) 185
Bse8I GATNNNNATC 1 cut(s) 602
BseBI CCWGG 2 cut(s) 273, 294
BseDI CCNNGG 4 cut(s) 143, 221, 403, 535
BseGI GGATG 3 cut(s) 62, 241, 488
BseJI GATNNNNATC 1 cut(s) 602
BseLI CCNNNNNNNGG 2 cut(s) 30, 409
BseMII CTCAG 3 cut(s) 81, 199, 842
BseNI ACTGG 2 cut(s) 547, 689
BseRI GAGGAG 2 cut(s) 572, 575
BseSI GKGCMC 2 cut(s) 416, 535
BseXI GCAGC 1 cut(s) 479
BsgI GTGCAG 1 cut(s) 106
BshFI GGCC 3 cut(s) 141, 533, 695
BsiHKAI GWGCWC 1 cut(s) 61
BsiHKCI CYCGRG 1 cut(s) 54
BsiSI CCGG 1 cut(s) 233
BslFI GGGAC 2 cut(s) 499, 553
BslI CCNNNNNNNGG 2 cut(s) 30, 409
BsmAI GTCTC 3 cut(s) 162, 575, 757
BsmFI GGGAC 2 cut(s) 499, 553
BsnI GGCC 3 cut(s) 141, 533, 695
Bso31I GGTCTC 1 cut(s) 757
BsoBI CYCGRG 1 cut(s) 54
Bsp120I GGGCCC 1 cut(s) 531
Bsp1286I GDGCHC 3 cut(s) 61, 416, 535
Bsp143I GATC 5 cut(s) 27, 79, 180, 354, 685
BspACI CCGC 2 cut(s) 340, 801
BspANI GGCC 3 cut(s) 141, 533, 695
BspCNI CTCAG 3 cut(s) 80, 198, 841
BspHI TCATGA 1 cut(s) 577
BspLI GGNNCC 1 cut(s) 533
BspPI GGATC 4 cut(s) 74, 175, 349, 680
BspTNI GGTCTC 1 cut(s) 757
BsrI ACTGG 2 cut(s) 547, 689
BssECI CCNNGG 4 cut(s) 143, 221, 403, 535
BssMI GATC 5 cut(s) 27, 79, 180, 354, 685
BssSI CACGAG 1 cut(s) 767
BssT1I CCWWGG 2 cut(s) 403, 535
Bst2BI CACGAG 1 cut(s) 767
Bst2UI CCWGG 2 cut(s) 273, 294
Bst4CI ACNGT 3 cut(s) 319, 761, 794
BstAPI GCANNNNNTGC 1 cut(s) 476
BstC8I GCNNGC 2 cut(s) 278, 370
BstDEI CTNAG 4 cut(s) 67, 185, 364, 828
BstDSI CCRYGG 2 cut(s) 143, 221
BstEII GGTNACC 1 cut(s) 761
BstF5I GGATG 3 cut(s) 62, 241, 488
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 5 cut(s) 30, 82, 183, 357, 688
BstMAI GTCTC 3 cut(s) 162, 575, 757
BstMBI GATC 5 cut(s) 27, 79, 180, 354, 685
BstMWI GCNNNNNNNGC 4 cut(s) 238, 247, 277, 476
BstNI CCWGG 2 cut(s) 273, 294
BstNSI RCATGY 1 cut(s) 372
BstPI GGTNACC 1 cut(s) 761
BstSCI CCNGG 2 cut(s) 271, 292
BstSFI CTRYAG 1 cut(s) 637
BstSLI GKGCMC 2 cut(s) 416, 535
BstV1I GCAGC 1 cut(s) 479
BstX2I RGATCY 3 cut(s) 27, 354, 685
BstYI RGATCY 3 cut(s) 27, 354, 685
Bsu36I CCTNAGG 1 cut(s) 185
BsuI GTATCC 2 cut(s) 540, 742
BsuRI GGCC 3 cut(s) 141, 533, 695
BtgI CCRYGG 2 cut(s) 143, 221
BtgZI GCGATG 1 cut(s) 383
BtsCI GGATG 3 cut(s) 62, 241, 488
BtsIMutI CAGTG 1 cut(s) 696
Cac8I GCNNGC 2 cut(s) 278, 370
CciI TCATGA 1 cut(s) 577
CfoI GCGC 1 cut(s) 241
Cfr13I GGNCC 3 cut(s) 140, 531, 532
CviAII CATG 5 cut(s) 117, 369, 449, 578, 798
DdeI CTNAG 4 cut(s) 67, 185, 364, 828
DpnI GATC 5 cut(s) 29, 81, 182, 356, 687
DpnII GATC 5 cut(s) 27, 79, 180, 354, 685
DraIII CACNNNGTG 1 cut(s) 769
EaeI YGGCCR 1 cut(s) 693
Eco130I CCWWGG 2 cut(s) 403, 535
Eco24I GRGCYC 1 cut(s) 535
Eco31I GGTCTC 1 cut(s) 757
Eco57I CTGAAG 2 cut(s) 402, 608
Eco81I CCTNAGG 1 cut(s) 185
Eco88I CYCGRG 1 cut(s) 54
Eco91I GGTNACC 1 cut(s) 761
EcoO109I RGGNCCY 2 cut(s) 531, 532
EcoO65I GGTNACC 1 cut(s) 761
EcoRII CCWGG 2 cut(s) 271, 292
EcoT14I CCWWGG 2 cut(s) 403, 535
EcoT38I GRGCYC 1 cut(s) 535
ErhI CCWWGG 2 cut(s) 403, 535
FaeI CATG 5 cut(s) 120, 372, 452, 581, 801
FalI AAGNNNNNCTT 2 cut(s) 400, 432
FaqI GGGAC 2 cut(s) 499, 553
FatI CATG 5 cut(s) 116, 368, 448, 577, 797
Fnu4HI GCNGC 2 cut(s) 341, 468
FokI GGATG 3 cut(s) 49, 248, 475
FriOI GRGCYC 1 cut(s) 535
Fsp4HI GCNGC 2 cut(s) 341, 468
FspBI CTAG 4 cut(s) 404, 536, 617, 683
GlaI GCGC 1 cut(s) 240
GluI GCNGC 2 cut(s) 341, 468
GsuI CTGGAG 2 cut(s) 272, 315
HaeIII GGCC 3 cut(s) 141, 533, 695
HapII CCGG 1 cut(s) 233
HhaI GCGC 1 cut(s) 241
Hin1II CATG 5 cut(s) 120, 372, 452, 581, 801
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HincII GTYRAC 1 cut(s) 21
HindII GTYRAC 1 cut(s) 21
HinfI GANTC 2 cut(s) 170, 583
HpaI GTTAAC 1 cut(s) 21
HpaII CCGG 1 cut(s) 233
HphI GGTGA 1 cut(s) 755
Hpy166II GTNNAC 2 cut(s) 21, 197
Hpy188I TCNGA 2 cut(s) 588, 831
Hpy188III TCNNGA 5 cut(s) 31, 420, 578, 607, 683
Hpy8I GTNNAC 2 cut(s) 21, 197
Hpy99I CGWCG 1 cut(s) 105
HpyAV CCTTC 5 cut(s) 21, 107, 402, 604, 858
HpyCH4III ACNGT 3 cut(s) 319, 761, 794
HpyCH4V TGCA 4 cut(s) 38, 87, 437, 467
HpyF10VI GCNNNNNNNGC 4 cut(s) 238, 247, 277, 476
HpyF3I CTNAG 4 cut(s) 67, 185, 364, 828
Hsp92II CATG 5 cut(s) 120, 372, 452, 581, 801
HspAI GCGC 1 cut(s) 239
KspAI GTTAAC 1 cut(s) 21
Kzo9I GATC 5 cut(s) 27, 79, 180, 354, 685
LmnI GCTCC 2 cut(s) 613, 620
Lsp1109I GCAGC 1 cut(s) 479
LweI GCATC 4 cut(s) 47, 226, 612, 861
MaeI CTAG 4 cut(s) 404, 536, 617, 683
MaeIII GTNAC 2 cut(s) 761, 811
MalI GATC 5 cut(s) 29, 81, 182, 356, 687
MboI GATC 5 cut(s) 27, 79, 180, 354, 685
MboII GAAGA 2 cut(s) 671, 794
MfeI CAATTG 1 cut(s) 200
MflI RGATCY 3 cut(s) 27, 354, 685
MhlI GDGCHC 3 cut(s) 61, 416, 535
MlsI TGGCCA 1 cut(s) 695
MluCI AATT 2 cut(s) 200, 739
MluNI TGGCCA 1 cut(s) 695
MlyI GAGTC 2 cut(s) 164, 577
MnlI CCTC 8 cut(s) 76, 131, 194, 290, 550, 553, 556, 776
Mox20I TGGCCA 1 cut(s) 695
MroXI GAANNNNTTC 1 cut(s) 786
MscI TGGCCA 1 cut(s) 695
MseI TTAA 1 cut(s) 20
MslI CAYNNNNRTG 1 cut(s) 701
Msp20I TGGCCA 1 cut(s) 695
MspI CCGG 1 cut(s) 233
MspR9I CCNGG 2 cut(s) 273, 294
MunI CAATTG 1 cut(s) 200
MvaI CCWGG 2 cut(s) 273, 294
MwoI GCNNNNNNNGC 4 cut(s) 238, 247, 277, 476
NdeII GATC 5 cut(s) 27, 79, 180, 354, 685
NlaIII CATG 5 cut(s) 120, 372, 452, 581, 801
NlaIV GGNNCC 1 cut(s) 533
NmeAIII GCCGAG 1 cut(s) 152
NmuCI GTSAC 1 cut(s) 761
NspI RCATGY 1 cut(s) 372
PaeI GCATGC 1 cut(s) 372
PagI TCATGA 1 cut(s) 577
PcsI WCGNNNNNNNCGW 1 cut(s) 765
PdmI GAANNNNTTC 1 cut(s) 786
PkrI GCNGC 2 cut(s) 342, 469
PleI GAGTC 2 cut(s) 164, 577
PpsI GAGTC 2 cut(s) 164, 577
Psp6I CCWGG 2 cut(s) 271, 292
PspEI GGTNACC 1 cut(s) 761
PspGI CCWGG 2 cut(s) 271, 292
PspN4I GGNNCC 1 cut(s) 533
PspOMI GGGCCC 1 cut(s) 531
PspPI GGNCC 3 cut(s) 140, 531, 532
PsuI RGATCY 3 cut(s) 27, 354, 685
RseI CAYNNNNRTG 1 cut(s) 701
SaqAI TTAA 1 cut(s) 20
SatI GCNGC 2 cut(s) 341, 468
Sau3AI GATC 5 cut(s) 27, 79, 180, 354, 685
Sau96I GGNCC 3 cut(s) 140, 531, 532
SchI GAGTC 2 cut(s) 164, 577
ScrFI CCNGG 2 cut(s) 273, 294
SduI GDGCHC 3 cut(s) 61, 416, 535
SfaNI GCATC 4 cut(s) 47, 226, 612, 861
SfcI CTRYAG 1 cut(s) 637
SmiMI CAYNNNNRTG 1 cut(s) 701
SmlI CTYRAG 1 cut(s) 847
SmoI CTYRAG 1 cut(s) 847
SphI GCATGC 1 cut(s) 372
Sse9I AATT 2 cut(s) 200, 739
SsiI CCGC 2 cut(s) 340, 801
SspMI CTAG 4 cut(s) 404, 536, 617, 683
StyD4I CCNGG 2 cut(s) 271, 292
StyI CCWWGG 2 cut(s) 403, 535
TaaI ACNGT 3 cut(s) 319, 761, 794
TaqI TCGA 2 cut(s) 100, 136
TasI AATT 2 cut(s) 200, 739
TauI GCSGC 1 cut(s) 343
Tru1I TTAA 1 cut(s) 20
Tru9I TTAA 1 cut(s) 20
TscAI CASTG 1 cut(s) 696
TseFI GTSAC 1 cut(s) 761
TseI GCWGC 1 cut(s) 467
Tsp45I GTSAC 1 cut(s) 761
TspDTI ATGAA 3 cut(s) 386, 617, 786
TspGWI ACGGA 4 cut(s) 72, 118, 210, 328
TspRI CASTG 1 cut(s) 696
XapI RAATTY 1 cut(s) 739
XbaI TCTAGA 1 cut(s) 682
XceI RCATGY 1 cut(s) 372
XmaJI CCTAGG 2 cut(s) 403, 535
XmnI GAANNNNTTC 1 cut(s) 786
XspI CTAG 4 cut(s) 404, 536, 617, 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.