Rh1BG281500

synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0004698
Physical Location & Seq
Reverse (-)
41835116 .. 41840199
5084 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG281500.1

Sequence Viewer

Length: 801 bp
ATGATTGCTTTGAATAAGCCAGGCGGGCTGCAAGTTTTACCTGGAGGTCTTTTTCAGCAACGGACAGTTCTAACACAGCTCCTATGGCGGCAAACTCAGACTCACTTAGCATGCCAAGAATCACATCCGGTTCCTGTTCATCGCCTAGGAAGGGGCACTTCAGGAATACTGCTATGTGCAAAGACGAAGCATGCCAAAACTCAACTTGCAGCATATTTTGCTGATGGGACATCCCATATTGGAGATGACAGTGACACCAACATGAAGCTGCATACAATAAGGAAAATCTCAAAGATATACCGGGCGCTAGTGACCGGGATACTTTGTGAGGACGAGGTGATTGTTAAGCAACCAATTGGAACTGTGCGATATCCTGGTGTGGCTAAAGGGCTGTATGTTGCTTCTCCCACAGGAAAACCAGCTCTGAGCAGAGTAGAAGTTCTTGAGAGGGACATACAGAAAAATCACACAATCGTACAGGTAGAGATTCAGTCAGGGAGACCACATCAAATACGCATTCATCTTTCTTTCATAGGACATCCTTTGCTAGGAGATCCTCTATATGTTGCTGGTGGACAACCAAAGTGCTTGGGATCTGACTTTGTGGATGAAAGTTTTGCAGATGATGGAGGGTTCAAGAGGCCTACGAAGCCTGTTCCTGGAGATTGTGGTTATAACTTGCATGCTCATCAAGTGGTTCTCTCTCACCCATCATCCAATGAGGGAATCAAAATTACAGCACCTCTCCCACTTGTCCTCCGGACTCGAGAAGAAATCGAAGAACTTAGTAGCGTTAGGTGA

Protein Analysis

266

Amino Acids

29.07

Weight (kDa)

9.08

Isoelectric Point (pI)

35.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 2 - 176 2e-22 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 675
AccIII TCCGGA 1 cut(s) 759
AciI CCGC 2 cut(s) 24, 88
AclWI GGATC 2 cut(s) 548, 601
AcuI CTGAAG 1 cut(s) 144
AfaI GTAC 1 cut(s) 477
AfiI CCNNNNNNNGG 3 cut(s) 151, 548, 659
AgsI TTSAA 2 cut(s) 13, 637
AjnI CCWGG 4 cut(s) 19, 40, 373, 658
AluBI AGCT 3 cut(s) 79, 268, 422
AluI AGCT 3 cut(s) 79, 268, 422
Alw26I GTCTC 1 cut(s) 493
AlwI GGATC 2 cut(s) 548, 601
Ama87I CYCGRG 1 cut(s) 765
Aor13HI TCCGGA 1 cut(s) 759
AoxI GGCC 1 cut(s) 641
ApeKI GCWGC 3 cut(s) 28, 209, 268
AspA2I CCTAGG 1 cut(s) 145
AspLEI GCGC 1 cut(s) 307
AsuC2I CCSGG 2 cut(s) 302, 316
AsuHPI GGTGA 2 cut(s) 349, 698
AvaI CYCGRG 1 cut(s) 765
AvrII CCTAGG 1 cut(s) 145
BaeGI GKGCMC 1 cut(s) 158
BbvI GCAGC 3 cut(s) 15, 221, 255
BccI CCATC 3 cut(s) 218, 620, 718
BciT130I CCWGG 4 cut(s) 21, 42, 375, 660
BciVI GTATCC 1 cut(s) 312
BcnI CCSGG 2 cut(s) 302, 316
BcoDI GTCTC 1 cut(s) 493
BfaI CTAG 3 cut(s) 146, 308, 548
BfoI RGCGCY 1 cut(s) 308
BfuI GTATCC 1 cut(s) 312
BglI GCCNNNNNGGC 1 cut(s) 25
BisI GCNGC 4 cut(s) 29, 89, 210, 269
BlnI CCTAGG 1 cut(s) 145
BlsI GCNGC 4 cut(s) 30, 90, 211, 270
Bme1390I CCNGG 6 cut(s) 21, 42, 302, 316, 375, 660
BmeT110I CYCGRG 1 cut(s) 765
BmiI GGNNCC 1 cut(s) 132
BmrFI CCNGG 6 cut(s) 21, 42, 302, 316, 375, 660
BpmI CTGGAG 2 cut(s) 63, 681
BpuEI CTTGAG 1 cut(s) 464
BpuMI CCSGG 2 cut(s) 302, 316
BsaI GGTCTC 1 cut(s) 493
BsaJI CCNNGG 1 cut(s) 145
BsaWI WCCGGW 2 cut(s) 127, 759
BsaXI ACNNNNNCTCC 2 cut(s) 741, 771
Bsc4I CCNNNNNNNGG 3 cut(s) 151, 548, 659
BseAI TCCGGA 1 cut(s) 759
BseBI CCWGG 4 cut(s) 21, 42, 375, 660
BseDI CCNNGG 1 cut(s) 145
BseGI GGATG 5 cut(s) 124, 230, 538, 613, 713
BseLI CCNNNNNNNGG 3 cut(s) 151, 548, 659
BseMII CTCAG 2 cut(s) 110, 416
BseSI GKGCMC 1 cut(s) 158
BseXI GCAGC 3 cut(s) 15, 221, 255
BshFI GGCC 1 cut(s) 643
BsiHKCI CYCGRG 1 cut(s) 765
BsiSI CCGG 4 cut(s) 128, 301, 315, 760
BslFI GGGAC 2 cut(s) 241, 464
BslI CCNNNNNNNGG 3 cut(s) 151, 548, 659
BsmAI GTCTC 1 cut(s) 493
BsmFI GGGAC 2 cut(s) 241, 464
BsmI GAATGC 1 cut(s) 516
BsnI GGCC 1 cut(s) 643
Bso31I GGTCTC 1 cut(s) 493
BsoBI CYCGRG 1 cut(s) 765
Bsp1286I GDGCHC 1 cut(s) 158
Bsp13I TCCGGA 1 cut(s) 759
Bsp143I GATC 2 cut(s) 553, 593
BspACI CCGC 2 cut(s) 24, 88
BspANI GGCC 1 cut(s) 643
BspCNI CTCAG 2 cut(s) 109, 417
BspEI TCCGGA 1 cut(s) 759
BspLI GGNNCC 1 cut(s) 132
BspPI GGATC 2 cut(s) 548, 601
BspTNI GGTCTC 1 cut(s) 493
BssECI CCNNGG 1 cut(s) 145
BssMI GATC 2 cut(s) 553, 593
BssT1I CCWWGG 1 cut(s) 145
Bst2UI CCWGG 4 cut(s) 21, 42, 375, 660
Bst4CI ACNGT 3 cut(s) 67, 251, 364
BstAPI GCANNNNNTGC 1 cut(s) 218
BstC8I GCNNGC 4 cut(s) 26, 112, 192, 684
BstDEI CTNAG 4 cut(s) 96, 106, 425, 785
BstENI CCTNNNNNAGG 1 cut(s) 546
BstF5I GGATG 5 cut(s) 124, 230, 538, 613, 713
BstH2I RGCGCY 1 cut(s) 308
BstHHI GCGC 1 cut(s) 307
BstKTI GATC 2 cut(s) 556, 596
BstMAI GTCTC 1 cut(s) 493
BstMBI GATC 2 cut(s) 553, 593
BstMWI GCNNNNNNNGC 4 cut(s) 25, 85, 218, 649
BstNI CCWGG 4 cut(s) 21, 42, 375, 660
BstNSI RCATGY 3 cut(s) 114, 194, 686
BstSCI CCNGG 6 cut(s) 19, 40, 300, 314, 373, 658
BstSLI GKGCMC 1 cut(s) 158
BstV1I GCAGC 3 cut(s) 15, 221, 255
BstX2I RGATCY 2 cut(s) 553, 593
BstYI RGATCY 2 cut(s) 553, 593
BsuI GTATCC 1 cut(s) 312
BsuRI GGCC 1 cut(s) 643
BtgZI GCGATG 1 cut(s) 125
BtsCI GGATG 5 cut(s) 124, 230, 538, 613, 713
BtsIMutI CAGTG 1 cut(s) 256
Cac8I GCNNGC 4 cut(s) 26, 112, 192, 684
CfoI GCGC 1 cut(s) 307
Csp6I GTAC 1 cut(s) 476
CviAII CATG 4 cut(s) 111, 191, 262, 683
CviJI RGCY 9 cut(s) 19, 28, 79, 268, 383, 391, 422, 643, 652
CviKI_1 RGCY 9 cut(s) 19, 28, 79, 268, 383, 391, 422, 643, 652
CviQI GTAC 1 cut(s) 476
DdeI CTNAG 4 cut(s) 96, 106, 425, 785
DpnI GATC 2 cut(s) 555, 595
DpnII GATC 2 cut(s) 553, 593
Eco130I CCWWGG 1 cut(s) 145
Eco147I AGGCCT 1 cut(s) 643
Eco31I GGTCTC 1 cut(s) 493
Eco32I GATATC 1 cut(s) 371
Eco57I CTGAAG 1 cut(s) 144
Eco88I CYCGRG 1 cut(s) 765
EcoNI CCTNNNNNAGG 1 cut(s) 546
EcoRII CCWGG 4 cut(s) 19, 40, 373, 658
EcoRV GATATC 1 cut(s) 371
EcoT14I CCWWGG 1 cut(s) 145
ErhI CCWWGG 1 cut(s) 145
FaeI CATG 4 cut(s) 114, 194, 265, 686
FalI AAGNNNNNCTT 2 cut(s) 142, 174
FaqI GGGAC 2 cut(s) 241, 464
FatI CATG 4 cut(s) 110, 190, 261, 682
FauI CCCGC 1 cut(s) 17
Fnu4HI GCNGC 4 cut(s) 29, 89, 210, 269
FokI GGATG 5 cut(s) 111, 217, 525, 620, 700
Fsp4HI GCNGC 4 cut(s) 29, 89, 210, 269
FspBI CTAG 3 cut(s) 146, 308, 548
GlaI GCGC 1 cut(s) 306
GluI GCNGC 4 cut(s) 29, 89, 210, 269
GsuI CTGGAG 2 cut(s) 63, 681
HaeII RGCGCY 1 cut(s) 308
HaeIII GGCC 1 cut(s) 643
HapII CCGG 4 cut(s) 128, 301, 315, 760
HhaI GCGC 1 cut(s) 307
Hin1II CATG 4 cut(s) 114, 194, 265, 686
Hin6I GCGC 1 cut(s) 305
HinP1I GCGC 1 cut(s) 305
HinfI GANTC 5 cut(s) 100, 119, 487, 726, 763
HpaII CCGG 4 cut(s) 128, 301, 315, 760
HphI GGTGA 2 cut(s) 349, 698
Hpy166II GTNNAC 1 cut(s) 575
Hpy188I TCNGA 3 cut(s) 99, 426, 598
Hpy188III TCNNGA 5 cut(s) 162, 443, 637, 760, 767
Hpy8I GTNNAC 1 cut(s) 575
HpyAV CCTTC 1 cut(s) 144
HpyCH4III ACNGT 3 cut(s) 67, 251, 364
HpyCH4V TGCA 6 cut(s) 31, 179, 209, 271, 620, 682
HpyF10VI GCNNNNNNNGC 4 cut(s) 25, 85, 218, 649
HpyF3I CTNAG 4 cut(s) 96, 106, 425, 785
Hsp92II CATG 4 cut(s) 114, 194, 265, 686
HspAI GCGC 1 cut(s) 305
Kpn2I TCCGGA 1 cut(s) 759
Kzo9I GATC 2 cut(s) 553, 593
LmnI GCTCC 1 cut(s) 84
Lsp1109I GCAGC 3 cut(s) 15, 221, 255
MaeI CTAG 3 cut(s) 146, 308, 548
MaeIII GTNAC 2 cut(s) 251, 310
MalI GATC 2 cut(s) 555, 595
MboI GATC 2 cut(s) 553, 593
MboII GAAGA 2 cut(s) 782, 791
MfeI CAATTG 1 cut(s) 354
MflI RGATCY 2 cut(s) 553, 593
MhlI GDGCHC 1 cut(s) 158
MluCI AATT 2 cut(s) 354, 732
MlyI GAGTC 2 cut(s) 94, 757
MroI TCCGGA 1 cut(s) 759
MseI TTAA 1 cut(s) 345
MslI CAYNNNNRTG 1 cut(s) 260
MspI CCGG 4 cut(s) 128, 301, 315, 760
MspR9I CCNGG 6 cut(s) 21, 42, 302, 316, 375, 660
MunI CAATTG 1 cut(s) 354
Mva1269I GAATGC 1 cut(s) 516
MvaI CCWGG 4 cut(s) 21, 42, 375, 660
MwoI GCNNNNNNNGC 4 cut(s) 25, 85, 218, 649
NciI CCSGG 2 cut(s) 302, 316
NdeII GATC 2 cut(s) 553, 593
NlaIII CATG 4 cut(s) 114, 194, 265, 686
NlaIV GGNNCC 1 cut(s) 132
NmuCI GTSAC 2 cut(s) 251, 310
NspI RCATGY 3 cut(s) 114, 194, 686
PaeI GCATGC 3 cut(s) 114, 194, 686
PaeR7I CTCGAG 1 cut(s) 765
PceI AGGCCT 1 cut(s) 643
PctI GAATGC 1 cut(s) 516
PfeI GAWTC 3 cut(s) 119, 487, 726
PfoI TCCNGGA 1 cut(s) 658
PkrI GCNGC 4 cut(s) 30, 90, 211, 270
PleI GAGTC 2 cut(s) 94, 757
PpsI GAGTC 2 cut(s) 94, 757
PsiI TTATAA 1 cut(s) 675
Psp6I CCWGG 4 cut(s) 19, 40, 373, 658
PspGI CCWGG 4 cut(s) 19, 40, 373, 658
PspN4I GGNNCC 1 cut(s) 132
PsuI RGATCY 2 cut(s) 553, 593
RsaI GTAC 1 cut(s) 477
RsaNI GTAC 1 cut(s) 476
RseI CAYNNNNRTG 1 cut(s) 260
SaqAI TTAA 1 cut(s) 345
SatI GCNGC 4 cut(s) 29, 89, 210, 269
Sau3AI GATC 2 cut(s) 553, 593
SchI GAGTC 2 cut(s) 94, 757
ScrFI CCNGG 6 cut(s) 21, 42, 302, 316, 375, 660
SduI GDGCHC 1 cut(s) 158
SetI ASST 9 cut(s) 43, 49, 81, 270, 339, 424, 483, 745, 800
Sfr274I CTCGAG 1 cut(s) 765
SlaI CTCGAG 1 cut(s) 765
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 2 cut(s) 443, 765
SmoI CTYRAG 2 cut(s) 443, 765
SphI GCATGC 3 cut(s) 114, 194, 686
Sse9I AATT 2 cut(s) 354, 732
SseBI AGGCCT 1 cut(s) 643
SsiI CCGC 2 cut(s) 24, 88
SspMI CTAG 3 cut(s) 146, 308, 548
StuI AGGCCT 1 cut(s) 643
StyD4I CCNGG 6 cut(s) 19, 40, 300, 314, 373, 658
StyI CCWWGG 1 cut(s) 145
TaaI ACNGT 3 cut(s) 67, 251, 364
TaqI TCGA 2 cut(s) 766, 777
TasI AATT 2 cut(s) 354, 732
TauI GCSGC 1 cut(s) 91
TfiI GAWTC 3 cut(s) 119, 487, 726
Tru1I TTAA 1 cut(s) 345
Tru9I TTAA 1 cut(s) 345
TscAI CASTG 1 cut(s) 256
TseFI GTSAC 2 cut(s) 251, 310
TseI GCWGC 3 cut(s) 28, 209, 268
Tsp45I GTSAC 2 cut(s) 251, 310
TspDTI ATGAA 5 cut(s) 128, 278, 509, 520, 624
TspGWI ACGGA 1 cut(s) 76
TspRI CASTG 1 cut(s) 256
XagI CCTNNNNNAGG 1 cut(s) 546
XceI RCATGY 3 cut(s) 114, 194, 686
XhoI CTCGAG 1 cut(s) 765
XmaJI CCTAGG 1 cut(s) 145
XspI CTAG 3 cut(s) 146, 308, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.