RchiOBHm_Chr5g0046841

synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0004698
Physical Location & Seq
Reverse (-)
43075348 .. 43081561
6214 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32481

Sequence Viewer

Length: 672 bp
ATGAGGTCTTCCACTCGGTTGGACTCTGCTCTGTTCCAACTCACACCCACTCGGACTAGCAATCATTGCTTTGTTGGTCTACAGATAACAGTTGATGGAGAAGTTATTAGAGACTCGAATACGATCCTCAGGGTTGGTTCACAATTGGTCTATCATAGGCTTCCGTGGAGAGAGCCGGATGCGCCATACTTGCTGGAGGTTCTGTATGAGGATGATGATATGCTTGCTTTGAATAAGCCAGGCGGGCTACAAGTTTTACCTGGAGGTCTTTTCCAGCAACATATAGTTCTAACACAGCTCCTATGGCGGCAAACTCAGAAGATCCATCACTTAGCATGCCAAAAATCACATCTTGTTCATGTTCATCGCCTAGGAAGGGGCACTTCAGGAATACTGCTAGGTGCAAAGATAAATCATGCCAAAAAGCAACTTGCAGCATATTTTGCTGATGGGACATCCCATATTGGAGATGACAGGAAGATCTCAAAGATATACCGGGCCCTAGGGACTGGGATACTTTGTGAGGAGGAGGTAATACTATCTCATGAGACTCTGAAGTTTGTGTGTGACTATGTCTTCATGTGCAATCTTAATGTTGGTATCTCAGAGACCATTTCTGTCCCATGTTTCTATAGCTTCTTTGATCTAGAAAGTTGCATACTTGGTTTTTAG

Protein Analysis

223

Amino Acids

25.1

Weight (kDa)

6.82

Isoelectric Point (pI)

37.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 74 - 170 2e-09 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 79
AciI CCGC 2 cut(s) 243, 307
AclWI GGATC 2 cut(s) 118, 316
AcuI CTGAAG 2 cut(s) 369, 575
AfiI CCNNNNNNNGG 1 cut(s) 376
AgsI TTSAA 1 cut(s) 232
AjnI CCWGG 2 cut(s) 238, 259
AluBI AGCT 2 cut(s) 298, 636
AluI AGCT 2 cut(s) 298, 636
Alw26I GTCTC 3 cut(s) 105, 542, 602
AlwI GGATC 2 cut(s) 118, 316
AoxI GGCC 1 cut(s) 498
ApaI GGGCCC 1 cut(s) 502
ApeKI GCWGC 1 cut(s) 434
AspA2I CCTAGG 2 cut(s) 370, 502
AspLEI GCGC 1 cut(s) 184
AspS9I GGNCC 2 cut(s) 498, 499
AsuC2I CCSGG 1 cut(s) 497
AvrII CCTAGG 2 cut(s) 370, 502
AxyI CCTNAGG 1 cut(s) 128
BaeGI GKGCMC 2 cut(s) 383, 502
BanII GRGCYC 1 cut(s) 502
BbsI GAAGAC 1 cut(s) 568
BbvI GCAGC 1 cut(s) 446
BccI CCATC 3 cut(s) 89, 333, 443
BciT130I CCWGG 2 cut(s) 240, 261
BciVI GTATCC 1 cut(s) 507
BcnI CCSGG 1 cut(s) 497
BcoDI GTCTC 3 cut(s) 105, 542, 602
BfaI CTAG 5 cut(s) 57, 371, 398, 503, 647
BfmI CTRYAG 2 cut(s) 80, 631
BfuI GTATCC 1 cut(s) 507
BglI GCCNNNNNGGC 1 cut(s) 244
BglII AGATCT 1 cut(s) 480
BisI GCNGC 2 cut(s) 308, 435
BlnI CCTAGG 2 cut(s) 370, 502
BlsI GCNGC 2 cut(s) 309, 436
Bme1390I CCNGG 3 cut(s) 240, 261, 497
BmgT120I GGNCC 2 cut(s) 498, 499
BmiI GGNNCC 1 cut(s) 500
BmrFI CCNGG 3 cut(s) 240, 261, 497
BmrI ACTGGG 1 cut(s) 519
BmsI GCATC 1 cut(s) 169
BmuI ACTGGG 1 cut(s) 519
BpiI GAAGAC 1 cut(s) 568
BpmI CTGGAG 2 cut(s) 215, 282
BpuMI CCSGG 1 cut(s) 497
BsaI GGTCTC 1 cut(s) 602
BsaJI CCNNGG 3 cut(s) 164, 370, 502
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bsc4I CCNNNNNNNGG 1 cut(s) 376
Bse1I ACTGG 1 cut(s) 514
Bse21I CCTNAGG 1 cut(s) 128
Bse3DI GCAATG 1 cut(s) 64
BseBI CCWGG 2 cut(s) 240, 261
BseDI CCNNGG 3 cut(s) 164, 370, 502
BseGI GGATG 3 cut(s) 184, 217, 455
BseLI CCNNNNNNNGG 1 cut(s) 376
BseMI GCAATG 1 cut(s) 64
BseMII CTCAG 3 cut(s) 142, 329, 618
BseNI ACTGG 1 cut(s) 514
BseRI GAGGAG 2 cut(s) 539, 542
BseSI GKGCMC 2 cut(s) 383, 502
BseXI GCAGC 1 cut(s) 446
BshFI GGCC 1 cut(s) 500
BsiSI CCGG 2 cut(s) 176, 496
BslFI GGGAC 3 cut(s) 466, 520, 605
BslI CCNNNNNNNGG 1 cut(s) 376
BsmAI GTCTC 3 cut(s) 105, 542, 602
BsmFI GGGAC 3 cut(s) 466, 520, 605
BsnI GGCC 1 cut(s) 500
Bso31I GGTCTC 1 cut(s) 602
Bsp120I GGGCCC 1 cut(s) 498
Bsp1286I GDGCHC 2 cut(s) 383, 502
Bsp143I GATC 4 cut(s) 123, 321, 480, 643
BspACI CCGC 2 cut(s) 243, 307
BspANI GGCC 1 cut(s) 500
BspCNI CTCAG 3 cut(s) 141, 328, 617
BspHI TCATGA 1 cut(s) 544
BspLI GGNNCC 1 cut(s) 500
BspPI GGATC 2 cut(s) 118, 316
BspTNI GGTCTC 1 cut(s) 602
BsrDI GCAATG 1 cut(s) 64
BsrI ACTGG 1 cut(s) 514
BssECI CCNNGG 3 cut(s) 164, 370, 502
BssMI GATC 4 cut(s) 123, 321, 480, 643
BssT1I CCWWGG 2 cut(s) 370, 502
Bst2UI CCWGG 2 cut(s) 240, 261
Bst4CI ACNGT 1 cut(s) 91
BstAPI GCANNNNNTGC 2 cut(s) 66, 443
BstC8I GCNNGC 3 cut(s) 225, 245, 337
BstDEI CTNAG 4 cut(s) 128, 315, 331, 604
BstDSI CCRYGG 1 cut(s) 164
BstF5I GGATG 3 cut(s) 184, 217, 455
BstHHI GCGC 1 cut(s) 184
BstKTI GATC 4 cut(s) 126, 324, 483, 646
BstMAI GTCTC 3 cut(s) 105, 542, 602
BstMBI GATC 4 cut(s) 123, 321, 480, 643
BstMWI GCNNNNNNNGC 6 cut(s) 66, 181, 190, 244, 304, 443
BstNI CCWGG 2 cut(s) 240, 261
BstNSI RCATGY 1 cut(s) 339
BstSCI CCNGG 3 cut(s) 238, 259, 495
BstSFI CTRYAG 2 cut(s) 80, 631
BstSLI GKGCMC 2 cut(s) 383, 502
BstV1I GCAGC 1 cut(s) 446
BstV2I GAAGAC 1 cut(s) 568
BstX2I RGATCY 2 cut(s) 321, 480
BstXI CCANNNNNNTGG 1 cut(s) 19
BstYI RGATCY 2 cut(s) 321, 480
Bsu36I CCTNAGG 1 cut(s) 128
BsuI GTATCC 1 cut(s) 507
BsuRI GGCC 1 cut(s) 500
BtgI CCRYGG 1 cut(s) 164
BtgZI GCGATG 1 cut(s) 350
BtsCI GGATG 3 cut(s) 184, 217, 455
Cac8I GCNNGC 3 cut(s) 225, 245, 337
CciI TCATGA 1 cut(s) 544
CfoI GCGC 1 cut(s) 184
Cfr13I GGNCC 2 cut(s) 498, 499
CviAII CATG 6 cut(s) 336, 359, 416, 545, 580, 624
CviJI RGCY 7 cut(s) 160, 175, 238, 247, 298, 500, 636
CviKI_1 RGCY 7 cut(s) 160, 175, 238, 247, 298, 500, 636
DdeI CTNAG 4 cut(s) 128, 315, 331, 604
DpnI GATC 4 cut(s) 125, 323, 482, 645
DpnII GATC 4 cut(s) 123, 321, 480, 643
Eco130I CCWWGG 2 cut(s) 370, 502
Eco24I GRGCYC 1 cut(s) 502
Eco31I GGTCTC 1 cut(s) 602
Eco57I CTGAAG 2 cut(s) 369, 575
Eco81I CCTNAGG 1 cut(s) 128
EcoO109I RGGNCCY 1 cut(s) 499
EcoRII CCWGG 2 cut(s) 238, 259
EcoT14I CCWWGG 2 cut(s) 370, 502
EcoT38I GRGCYC 1 cut(s) 502
ErhI CCWWGG 2 cut(s) 370, 502
FaeI CATG 6 cut(s) 339, 362, 419, 548, 583, 627
FalI AAGNNNNNCTT 2 cut(s) 367, 399
FaqI GGGAC 3 cut(s) 466, 520, 605
FatI CATG 6 cut(s) 335, 358, 415, 544, 579, 623
FauI CCCGC 1 cut(s) 236
FblI GTMKAC 1 cut(s) 79
Fnu4HI GCNGC 2 cut(s) 308, 435
FokI GGATG 3 cut(s) 191, 224, 442
FriOI GRGCYC 1 cut(s) 502
Fsp4HI GCNGC 2 cut(s) 308, 435
FspBI CTAG 5 cut(s) 57, 371, 398, 503, 647
GlaI GCGC 1 cut(s) 183
GluI GCNGC 2 cut(s) 308, 435
GsuI CTGGAG 2 cut(s) 215, 282
HaeIII GGCC 1 cut(s) 500
HapII CCGG 2 cut(s) 176, 496
HhaI GCGC 1 cut(s) 184
Hin1II CATG 6 cut(s) 339, 362, 419, 548, 583, 627
Hin6I GCGC 1 cut(s) 182
HinP1I GCGC 1 cut(s) 182
HinfI GANTC 3 cut(s) 23, 113, 550
HpaII CCGG 2 cut(s) 176, 496
Hpy166II GTNNAC 2 cut(s) 80, 140
Hpy188I TCNGA 4 cut(s) 54, 318, 555, 607
Hpy188III TCNNGA 3 cut(s) 387, 545, 647
Hpy8I GTNNAC 2 cut(s) 80, 140
HpyAV CCTTC 1 cut(s) 369
HpyCH4III ACNGT 1 cut(s) 91
HpyCH4V TGCA 4 cut(s) 404, 434, 585, 657
HpyF10VI GCNNNNNNNGC 6 cut(s) 66, 181, 190, 244, 304, 443
HpyF3I CTNAG 4 cut(s) 128, 315, 331, 604
Hsp92II CATG 6 cut(s) 339, 362, 419, 548, 583, 627
HspAI GCGC 1 cut(s) 182
Kzo9I GATC 4 cut(s) 123, 321, 480, 643
LmnI GCTCC 1 cut(s) 303
Lsp1109I GCAGC 1 cut(s) 446
LweI GCATC 1 cut(s) 169
MaeI CTAG 5 cut(s) 57, 371, 398, 503, 647
MaeIII GTNAC 1 cut(s) 566
MalI GATC 4 cut(s) 125, 323, 482, 645
MboI GATC 4 cut(s) 123, 321, 480, 643
MboII GAAGA 3 cut(s) 331, 490, 568
MfeI CAATTG 1 cut(s) 143
MflI RGATCY 2 cut(s) 321, 480
MhlI GDGCHC 2 cut(s) 383, 502
MluCI AATT 1 cut(s) 143
MlyI GAGTC 3 cut(s) 17, 107, 544
MmeI TCCRAC 1 cut(s) 61
MnlI CCTC 7 cut(s) 137, 190, 202, 257, 517, 520, 523
MseI TTAA 1 cut(s) 591
MspI CCGG 2 cut(s) 176, 496
MspR9I CCNGG 3 cut(s) 240, 261, 497
MunI CAATTG 1 cut(s) 143
MvaI CCWGG 2 cut(s) 240, 261
MwoI GCNNNNNNNGC 6 cut(s) 66, 181, 190, 244, 304, 443
NciI CCSGG 1 cut(s) 497
NdeII GATC 4 cut(s) 123, 321, 480, 643
NlaIII CATG 6 cut(s) 339, 362, 419, 548, 583, 627
NlaIV GGNNCC 1 cut(s) 500
NmuCI GTSAC 1 cut(s) 566
NspI RCATGY 1 cut(s) 339
PaeI GCATGC 1 cut(s) 339
PagI TCATGA 1 cut(s) 544
PflFI GACNNNGTC 1 cut(s) 572
PkrI GCNGC 2 cut(s) 309, 436
PleI GAGTC 3 cut(s) 17, 107, 544
PpsI GAGTC 3 cut(s) 17, 107, 544
Psp6I CCWGG 2 cut(s) 238, 259
PspGI CCWGG 2 cut(s) 238, 259
PspN4I GGNNCC 1 cut(s) 500
PspOMI GGGCCC 1 cut(s) 498
PspPI GGNCC 2 cut(s) 498, 499
PsuI RGATCY 2 cut(s) 321, 480
PsyI GACNNNGTC 1 cut(s) 572
SaqAI TTAA 1 cut(s) 591
SatI GCNGC 2 cut(s) 308, 435
Sau3AI GATC 4 cut(s) 123, 321, 480, 643
Sau96I GGNCC 2 cut(s) 498, 499
SchI GAGTC 3 cut(s) 17, 107, 544
ScrFI CCNGG 3 cut(s) 240, 261, 497
SduI GDGCHC 2 cut(s) 383, 502
SetI ASST 8 cut(s) 8, 201, 262, 268, 300, 403, 534, 638
SfaNI GCATC 1 cut(s) 169
SfcI CTRYAG 2 cut(s) 80, 631
SphI GCATGC 1 cut(s) 339
Sse9I AATT 1 cut(s) 143
SsiI CCGC 2 cut(s) 243, 307
SspMI CTAG 5 cut(s) 57, 371, 398, 503, 647
StyD4I CCNGG 3 cut(s) 238, 259, 495
StyI CCWWGG 2 cut(s) 370, 502
TaaI ACNGT 1 cut(s) 91
TaqI TCGA 1 cut(s) 116
TasI AATT 1 cut(s) 143
TauI GCSGC 1 cut(s) 310
Tru1I TTAA 1 cut(s) 591
Tru9I TTAA 1 cut(s) 591
TseFI GTSAC 1 cut(s) 566
TseI GCWGC 1 cut(s) 434
Tsp45I GTSAC 1 cut(s) 566
TspDTI ATGAA 3 cut(s) 347, 353, 568
TspGWI ACGGA 1 cut(s) 153
Tth111I GACNNNGTC 1 cut(s) 572
XbaI TCTAGA 1 cut(s) 646
XceI RCATGY 1 cut(s) 339
XmaJI CCTAGG 2 cut(s) 370, 502
XmiI GTMKAC 1 cut(s) 79
XspI CTAG 5 cut(s) 57, 371, 398, 503, 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.