Rh4CG111400

synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0004698
Physical Location & Seq
Reverse (-)
20930758 .. 20935781
5024 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG111400.1

Sequence Viewer

Length: 300 bp
ATGGTGACTGTGCTTGACATATCATTGCTAATATGTGTTCAGATTGCTTTGAATAAGCCAGGCAGGCTACAAGTTTTACCTGGAGATCTTTTCCAGCAACGGACAGTTCTAACACAACTCCTATGGCGGCAAACTCAAAAGATCCATCACTTAGCATGCCAAAAATCACATCTTGTTCCTGTTCATCGCCTAGGAAGGGGCACTTCAGGAATACTGCTATGTGCAAAGATAAATCATGCCAAAAAGCAACTTGCAGCATATTTTGCTGATGGGACATCCATATTGGAGATGACAGGTTAA

Protein Analysis

99

Amino Acids

10.98

Weight (kDa)

9.94

Isoelectric Point (pI)

27.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 15 - 90 2.3e-07 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 127
AclWI GGATC 1 cut(s) 136
AcuI CTGAAG 1 cut(s) 189
AfiI CCNNNNNNNGG 1 cut(s) 196
AgsI TTSAA 1 cut(s) 52
AjnI CCWGG 2 cut(s) 58, 79
AlwI GGATC 1 cut(s) 136
ApeKI GCWGC 1 cut(s) 254
AspA2I CCTAGG 1 cut(s) 190
AsuHPI GGTGA 1 cut(s) 16
AvrII CCTAGG 1 cut(s) 190
BaeGI GKGCMC 1 cut(s) 203
BbvI GCAGC 1 cut(s) 266
BccI CCATC 2 cut(s) 153, 263
BciT130I CCWGG 2 cut(s) 60, 81
BfaI CTAG 1 cut(s) 191
BglI GCCNNNNNGGC 1 cut(s) 64
BglII AGATCT 1 cut(s) 85
BisI GCNGC 2 cut(s) 128, 255
BlnI CCTAGG 1 cut(s) 190
BlsI GCNGC 2 cut(s) 129, 256
Bme1390I CCNGG 2 cut(s) 60, 81
BmrFI CCNGG 2 cut(s) 60, 81
BpmI CTGGAG 1 cut(s) 102
BsaJI CCNNGG 1 cut(s) 190
Bsc4I CCNNNNNNNGG 1 cut(s) 196
Bse3DI GCAATG 1 cut(s) 23
BseBI CCWGG 2 cut(s) 60, 81
BseDI CCNNGG 1 cut(s) 190
BseGI GGATG 1 cut(s) 275
BseLI CCNNNNNNNGG 1 cut(s) 196
BseMI GCAATG 1 cut(s) 23
BseSI GKGCMC 1 cut(s) 203
BseXI GCAGC 1 cut(s) 266
BslFI GGGAC 1 cut(s) 286
BslI CCNNNNNNNGG 1 cut(s) 196
BsmFI GGGAC 1 cut(s) 286
Bsp1286I GDGCHC 1 cut(s) 203
Bsp143I GATC 2 cut(s) 85, 141
BspACI CCGC 1 cut(s) 127
BspPI GGATC 1 cut(s) 136
BsrDI GCAATG 1 cut(s) 23
BssECI CCNNGG 1 cut(s) 190
BssMI GATC 2 cut(s) 85, 141
BssT1I CCWWGG 1 cut(s) 190
Bst2UI CCWGG 2 cut(s) 60, 81
Bst4CI ACNGT 2 cut(s) 10, 106
BstAPI GCANNNNNTGC 1 cut(s) 263
BstC8I GCNNGC 2 cut(s) 65, 157
BstDEI CTNAG 1 cut(s) 151
BstF5I GGATG 1 cut(s) 275
BstKTI GATC 2 cut(s) 88, 144
BstMBI GATC 2 cut(s) 85, 141
BstMWI GCNNNNNNNGC 2 cut(s) 64, 263
BstNI CCWGG 2 cut(s) 60, 81
BstNSI RCATGY 1 cut(s) 159
BstSCI CCNGG 2 cut(s) 58, 79
BstSLI GKGCMC 1 cut(s) 203
BstV1I GCAGC 1 cut(s) 266
BstX2I RGATCY 2 cut(s) 85, 141
BstYI RGATCY 2 cut(s) 85, 141
BtgZI GCGATG 1 cut(s) 170
BtsCI GGATG 1 cut(s) 275
Cac8I GCNNGC 2 cut(s) 65, 157
CviAII CATG 2 cut(s) 156, 236
CviJI RGCY 2 cut(s) 58, 67
CviKI_1 RGCY 2 cut(s) 58, 67
DdeI CTNAG 1 cut(s) 151
DpnI GATC 2 cut(s) 87, 143
DpnII GATC 2 cut(s) 85, 141
Eco130I CCWWGG 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 189
EcoRII CCWGG 2 cut(s) 58, 79
EcoT14I CCWWGG 1 cut(s) 190
ErhI CCWWGG 1 cut(s) 190
FaeI CATG 2 cut(s) 159, 239
FaiI YATR 8 cut(s) 20, 34, 124, 157, 220, 237, 259, 281
FalI AAGNNNNNCTT 2 cut(s) 187, 219
FaqI GGGAC 1 cut(s) 286
FatI CATG 2 cut(s) 155, 235
Fnu4HI GCNGC 2 cut(s) 128, 255
FokI GGATG 1 cut(s) 262
Fsp4HI GCNGC 2 cut(s) 128, 255
FspBI CTAG 1 cut(s) 191
GluI GCNGC 2 cut(s) 128, 255
GsuI CTGGAG 1 cut(s) 102
Hin1II CATG 2 cut(s) 159, 239
HphI GGTGA 1 cut(s) 16
Hpy188I TCNGA 1 cut(s) 42
Hpy188III TCNNGA 1 cut(s) 207
HpyAV CCTTC 1 cut(s) 189
HpyCH4III ACNGT 2 cut(s) 10, 106
HpyCH4V TGCA 2 cut(s) 224, 254
HpyF10VI GCNNNNNNNGC 2 cut(s) 64, 263
HpyF3I CTNAG 1 cut(s) 151
Hsp92II CATG 2 cut(s) 159, 239
Kzo9I GATC 2 cut(s) 85, 141
LpnPI CCDG 9 cut(s) 45, 49, 66, 72, 93, 107, 192, 192, 279
Lsp1109I GCAGC 1 cut(s) 266
MaeI CTAG 1 cut(s) 191
MaeIII GTNAC 1 cut(s) 4
MalI GATC 2 cut(s) 87, 143
MboI GATC 2 cut(s) 85, 141
MflI RGATCY 2 cut(s) 85, 141
MhlI GDGCHC 1 cut(s) 203
MseI TTAA 1 cut(s) 298
MspR9I CCNGG 2 cut(s) 60, 81
MvaI CCWGG 2 cut(s) 60, 81
MwoI GCNNNNNNNGC 2 cut(s) 64, 263
NdeII GATC 2 cut(s) 85, 141
NlaIII CATG 2 cut(s) 159, 239
NmuCI GTSAC 1 cut(s) 4
NspI RCATGY 1 cut(s) 159
PaeI GCATGC 1 cut(s) 159
PkrI GCNGC 2 cut(s) 129, 256
Psp6I CCWGG 2 cut(s) 58, 79
PspGI CCWGG 2 cut(s) 58, 79
PsuI RGATCY 2 cut(s) 85, 141
SaqAI TTAA 1 cut(s) 298
SatI GCNGC 2 cut(s) 128, 255
Sau3AI GATC 2 cut(s) 85, 141
ScrFI CCNGG 2 cut(s) 60, 81
SduI GDGCHC 1 cut(s) 203
SetI ASST 2 cut(s) 82, 298
SphI GCATGC 1 cut(s) 159
SsiI CCGC 1 cut(s) 127
SspMI CTAG 1 cut(s) 191
StyD4I CCNGG 2 cut(s) 58, 79
StyI CCWWGG 1 cut(s) 190
TaaI ACNGT 2 cut(s) 10, 106
TauI GCSGC 1 cut(s) 130
Tru1I TTAA 1 cut(s) 298
Tru9I TTAA 1 cut(s) 298
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 1 cut(s) 254
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 1 cut(s) 173
TspGWI ACGGA 1 cut(s) 115
XceI RCATGY 1 cut(s) 159
XmaJI CCTAGG 1 cut(s) 190
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.