Rh1CG297900

synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0004698
Physical Location & Seq
Reverse (-)
57267949 .. 57272598
4650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG297900.1

Sequence Viewer

Length: 1128 bp
ATGTCGACCTCTTCGCTATCACCTCAGACTTTCGGATTGCCGTGGCCCGATCTCAACGACGGCTTGTCATACAACGACGTCGTTAAACCCTCCGATTCCGAATTGACACTGATAGAGTTCTACTCTAGCAAATACAAGAGCTCAGCTCCGTTGCAAGGTTGGCTGCAGAGAATCCGAAACGGACAGATAACAGTTGATGGAGAAGTAGTTAGAGATTCGAATACGATTCTCAGGGTTGGTTCGGAATTGGTCTATCATAGGCTTGCATGGAGAGAGCCGGATGCGCCATACTTGCTCGAGGTTCTATATGAGGATGATGATATGATTGCTTTGAATAAGCCAGGCGGGCTGCAAGTTTTACCTGGAGGTCTTTTTCAGCAACGGACAGTTCTAACACAGCTCCTATGGCGGCAAACTCAGAAGATCCATCACTTAGCATGCCAAGAATCACATCCAGTTCCTGTTCATCGCCTAGGAAGGGGCACTTCAGGAATACTGCTATGTGCAAAGACGAAGCATGCCAAAACTCAACTTGCAGCATATTTTGCTGATGGGACATCCCATATTGGAGATGACAGTGACACCAACATGAAGCTGCATACAATAAGGAAAATCTCAAAGATATACCGGGCGCTAGTGACCGGGATACTTTGTGAGGACAAGGTGATTGTTAAGCAACCAATTGGAACTGTGCGATATCCTGGTGTGGCTAAAGGGCTGTATGTTGCTTCTCCCACAGGAAAACCAGCTCTGAGCAAAGTAGAAGTTCTTGAGAGGGACATACAGAAAAATCACACAATCGTACAGGTAGAGATTCAGTCAGGGAGACCACATCAAATACGCATTCATCTTTCTTTCATAGGACATCCCTTGCTAGGAGATCTTCTATATGTTGCTGGTGGACAACCAAAGTGCTTGGGATCTGACTTTGTAGATGAAAGTTTTGCAGATGACGGAGGGTTCAAGAGGCCTACGAAGCCTGTTCCTGGAGATTGTGGTTATAACTTGCATGCTCATCAAGTGGTTCTCTCTCACCCATCATCCAATGAGGTAATCAAAATTACAGCACCTCTCCCACTTGTCCTCCGGACTCGAGAAGAAATCGAAGAACTTAGTAGCGTTATGTGA

Protein Analysis

375

Amino Acids

41.5

Weight (kDa)

7.26

Isoelectric Point (pI)

40.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 108 - 285 7.3e-22 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1002
AatII GACGTC 1 cut(s) 81
AccI GTMKAC 1 cut(s) 5
AccIII TCCGGA 1 cut(s) 1086
AciI CCGC 2 cut(s) 345, 409
AclWI GGATC 2 cut(s) 418, 928
AcuI CTGAAG 1 cut(s) 471
AcyI GRCGYC 1 cut(s) 78
AfaI GTAC 1 cut(s) 804
AfiI CCNNNNNNNGG 4 cut(s) 155, 478, 875, 986
AgsI TTSAA 2 cut(s) 334, 964
AhdI GACNNNNNGTC 1 cut(s) 64
AjnI CCWGG 4 cut(s) 340, 361, 700, 985
AluBI AGCT 5 cut(s) 141, 146, 400, 595, 749
AluI AGCT 5 cut(s) 141, 146, 400, 595, 749
Alw21I GWGCWC 1 cut(s) 143
Alw26I GTCTC 1 cut(s) 820
AlwI GGATC 2 cut(s) 418, 928
AlwNI CAGNNNCTG 1 cut(s) 461
Ama87I CYCGRG 2 cut(s) 296, 1092
Aor13HI TCCGGA 1 cut(s) 1086
AoxI GGCC 2 cut(s) 44, 968
ApeKI GCWGC 4 cut(s) 163, 349, 536, 595
AspA2I CCTAGG 1 cut(s) 472
AspLEI GCGC 2 cut(s) 286, 634
AspS9I GGNCC 1 cut(s) 45
AsuC2I CCSGG 2 cut(s) 629, 643
AsuHPI GGTGA 3 cut(s) 12, 676, 1025
AsuII TTCGAA 1 cut(s) 218
AvaI CYCGRG 2 cut(s) 296, 1092
AvrII CCTAGG 1 cut(s) 472
BaeGI GKGCMC 1 cut(s) 485
BanII GRGCYC 1 cut(s) 143
Bbv12I GWGCWC 1 cut(s) 143
BbvI GCAGC 4 cut(s) 150, 336, 548, 582
BccI CCATC 4 cut(s) 191, 435, 545, 1045
BceAI ACGGC 2 cut(s) 25, 76
BciT130I CCWGG 4 cut(s) 342, 363, 702, 987
BciVI GTATCC 1 cut(s) 639
BcnI CCSGG 2 cut(s) 629, 643
BcoDI GTCTC 1 cut(s) 820
BfaI CTAG 4 cut(s) 126, 473, 635, 875
BfmI CTRYAG 1 cut(s) 164
BfoI RGCGCY 1 cut(s) 635
BfuI GTATCC 1 cut(s) 639
BglI GCCNNNNNGGC 1 cut(s) 346
BglII AGATCT 1 cut(s) 880
BisI GCNGC 5 cut(s) 164, 350, 410, 537, 596
BlnI CCTAGG 1 cut(s) 472
BlpI GCTNAGC 1 cut(s) 142
BlsI GCNGC 5 cut(s) 165, 351, 411, 538, 597
Bme1390I CCNGG 6 cut(s) 342, 363, 629, 643, 702, 987
BmeRI GACNNNNNGTC 1 cut(s) 64
BmeT110I CYCGRG 2 cut(s) 296, 1092
BmgT120I GGNCC 1 cut(s) 45
BmrFI CCNGG 6 cut(s) 342, 363, 629, 643, 702, 987
BmsI GCATC 1 cut(s) 271
BplI GAGNNNNNCTC 4 cut(s) 107, 139, 130, 162
BpmI CTGGAG 2 cut(s) 384, 1008
Bpu1102I GCTNAGC 1 cut(s) 142
Bpu14I TTCGAA 1 cut(s) 218
BpuEI CTTGAG 1 cut(s) 791
BpuMI CCSGG 2 cut(s) 629, 643
BsaHI GRCGYC 1 cut(s) 78
BsaI GGTCTC 1 cut(s) 820
BsaJI CCNNGG 2 cut(s) 41, 472
BsaWI WCCGGW 1 cut(s) 1086
BsaXI ACNNNNNCTCC 4 cut(s) 192, 222, 1068, 1098
Bsc4I CCNNNNNNNGG 4 cut(s) 155, 478, 875, 986
Bse1I ACTGG 1 cut(s) 455
BseAI TCCGGA 1 cut(s) 1086
BseBI CCWGG 4 cut(s) 342, 363, 702, 987
BseDI CCNNGG 2 cut(s) 41, 472
BseGI GGATG 6 cut(s) 286, 319, 451, 557, 865, 1040
BseLI CCNNNNNNNGG 4 cut(s) 155, 478, 875, 986
BseMII CTCAG 5 cut(s) 38, 156, 244, 431, 743
BseNI ACTGG 1 cut(s) 455
BseSI GKGCMC 1 cut(s) 485
BseXI GCAGC 4 cut(s) 150, 336, 548, 582
BshFI GGCC 2 cut(s) 46, 970
BsiHKAI GWGCWC 1 cut(s) 143
BsiHKCI CYCGRG 2 cut(s) 296, 1092
BsiSI CCGG 4 cut(s) 278, 628, 642, 1087
BslFI GGGAC 2 cut(s) 568, 791
BslI CCNNNNNNNGG 4 cut(s) 155, 478, 875, 986
BsmAI GTCTC 1 cut(s) 820
BsmFI GGGAC 2 cut(s) 568, 791
BsmI GAATGC 1 cut(s) 843
BsnI GGCC 2 cut(s) 46, 970
Bso31I GGTCTC 1 cut(s) 820
BsoBI CYCGRG 2 cut(s) 296, 1092
Bsp119I TTCGAA 1 cut(s) 218
Bsp1286I GDGCHC 2 cut(s) 143, 485
Bsp13I TCCGGA 1 cut(s) 1086
Bsp143I GATC 4 cut(s) 49, 423, 880, 920
Bsp1720I GCTNAGC 1 cut(s) 142
BspACI CCGC 2 cut(s) 345, 409
BspANI GGCC 2 cut(s) 46, 970
BspCNI CTCAG 5 cut(s) 37, 155, 243, 430, 744
BspEI TCCGGA 1 cut(s) 1086
BspMAI CTGCAG 1 cut(s) 168
BspPI GGATC 2 cut(s) 418, 928
BspT104I TTCGAA 1 cut(s) 218
BspTNI GGTCTC 1 cut(s) 820
BsrI ACTGG 1 cut(s) 455
BssECI CCNNGG 2 cut(s) 41, 472
BssMI GATC 4 cut(s) 49, 423, 880, 920
BssNI GRCGYC 1 cut(s) 78
BssT1I CCWWGG 1 cut(s) 472
Bst2UI CCWGG 4 cut(s) 342, 363, 702, 987
Bst4CI ACNGT 4 cut(s) 193, 388, 578, 691
Bst6I CTCTTC 1 cut(s) 16
BstACI GRCGYC 1 cut(s) 78
BstAPI GCANNNNNTGC 1 cut(s) 545
BstBI TTCGAA 1 cut(s) 218
BstC8I GCNNGC 5 cut(s) 264, 347, 439, 519, 1011
BstDEI CTNAG 7 cut(s) 24, 142, 230, 417, 433, 752, 1112
BstDSI CCRYGG 1 cut(s) 41
BstF5I GGATG 6 cut(s) 286, 319, 451, 557, 865, 1040
BstH2I RGCGCY 1 cut(s) 635
BstHHI GCGC 2 cut(s) 286, 634
BstKTI GATC 4 cut(s) 52, 426, 883, 923
BstMAI GTCTC 1 cut(s) 820
BstMBI GATC 4 cut(s) 49, 423, 880, 920
BstMWI GCNNNNNNNGC 7 cut(s) 160, 283, 292, 346, 406, 545, 976
BstNI CCWGG 4 cut(s) 342, 363, 702, 987
BstNSI RCATGY 3 cut(s) 441, 521, 1013
BstSCI CCNGG 6 cut(s) 340, 361, 627, 641, 700, 985
BstSFI CTRYAG 1 cut(s) 164
BstSLI GKGCMC 1 cut(s) 485
BstV1I GCAGC 4 cut(s) 150, 336, 548, 582
BstX2I RGATCY 3 cut(s) 423, 880, 920
BstYI RGATCY 3 cut(s) 423, 880, 920
BsuI GTATCC 1 cut(s) 639
BsuRI GGCC 2 cut(s) 46, 970
BtgI CCRYGG 1 cut(s) 41
BtgZI GCGATG 1 cut(s) 452
BtsCI GGATG 6 cut(s) 286, 319, 451, 557, 865, 1040
BtsIMutI CAGTG 2 cut(s) 107, 583
Cac8I GCNNGC 5 cut(s) 264, 347, 439, 519, 1011
CaiI CAGNNNCTG 1 cut(s) 461
CfoI GCGC 2 cut(s) 286, 634
Cfr13I GGNCC 1 cut(s) 45
Csp6I GTAC 1 cut(s) 803
CviAII CATG 5 cut(s) 267, 438, 518, 589, 1010
CviQI GTAC 1 cut(s) 803
DdeI CTNAG 7 cut(s) 24, 142, 230, 417, 433, 752, 1112
DpnI GATC 4 cut(s) 51, 425, 882, 922
DpnII GATC 4 cut(s) 49, 423, 880, 920
DriI GACNNNNNGTC 1 cut(s) 64
Eam1104I CTCTTC 1 cut(s) 16
Eam1105I GACNNNNNGTC 1 cut(s) 64
EarI CTCTTC 1 cut(s) 16
Ecl136II GAGCTC 1 cut(s) 141
Eco130I CCWWGG 1 cut(s) 472
Eco147I AGGCCT 1 cut(s) 970
Eco24I GRGCYC 1 cut(s) 143
Eco31I GGTCTC 1 cut(s) 820
Eco32I GATATC 1 cut(s) 698
Eco53kI GAGCTC 1 cut(s) 141
Eco57I CTGAAG 1 cut(s) 471
Eco88I CYCGRG 2 cut(s) 296, 1092
EcoICRI GAGCTC 1 cut(s) 141
EcoRII CCWGG 4 cut(s) 340, 361, 700, 985
EcoRV GATATC 1 cut(s) 698
EcoT14I CCWWGG 1 cut(s) 472
EcoT38I GRGCYC 1 cut(s) 143
ErhI CCWWGG 1 cut(s) 472
FaeI CATG 5 cut(s) 270, 441, 521, 592, 1013
FalI AAGNNNNNCTT 2 cut(s) 469, 501
FaqI GGGAC 2 cut(s) 568, 791
FatI CATG 5 cut(s) 266, 437, 517, 588, 1009
FauI CCCGC 1 cut(s) 338
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 5 cut(s) 164, 350, 410, 537, 596
FokI GGATG 6 cut(s) 293, 326, 438, 544, 852, 1027
FriOI GRGCYC 1 cut(s) 143
Fsp4HI GCNGC 5 cut(s) 164, 350, 410, 537, 596
FspBI CTAG 4 cut(s) 126, 473, 635, 875
GlaI GCGC 2 cut(s) 285, 633
GluI GCNGC 5 cut(s) 164, 350, 410, 537, 596
GsuI CTGGAG 2 cut(s) 384, 1008
HaeII RGCGCY 1 cut(s) 635
HaeIII GGCC 2 cut(s) 46, 970
HapII CCGG 4 cut(s) 278, 628, 642, 1087
HhaI GCGC 2 cut(s) 286, 634
Hin1I GRCGYC 1 cut(s) 78
Hin1II CATG 5 cut(s) 270, 441, 521, 592, 1013
Hin6I GCGC 2 cut(s) 284, 632
HinP1I GCGC 2 cut(s) 284, 632
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 7 cut(s) 95, 171, 215, 226, 446, 814, 1090
HpaII CCGG 4 cut(s) 278, 628, 642, 1087
HphI GGTGA 3 cut(s) 12, 676, 1025
Hpy166II GTNNAC 2 cut(s) 6, 902
Hpy188I TCNGA 9 cut(s) 27, 35, 94, 100, 176, 244, 420, 753, 925
Hpy188III TCNNGA 5 cut(s) 489, 770, 964, 1087, 1094
Hpy8I GTNNAC 2 cut(s) 6, 902
Hpy99I CGWCG 3 cut(s) 62, 80, 83
HpyAV CCTTC 1 cut(s) 471
HpyCH4III ACNGT 4 cut(s) 193, 388, 578, 691
HpyCH4IV ACGT 1 cut(s) 78
HpyCH4V TGCA 9 cut(s) 154, 166, 266, 352, 506, 536, 598, 947, 1009
HpyF10VI GCNNNNNNNGC 7 cut(s) 160, 283, 292, 346, 406, 545, 976
HpyF3I CTNAG 7 cut(s) 24, 142, 230, 417, 433, 752, 1112
HpySE526I ACGT 1 cut(s) 78
Hsp92I GRCGYC 1 cut(s) 78
Hsp92II CATG 5 cut(s) 270, 441, 521, 592, 1013
HspAI GCGC 2 cut(s) 284, 632
Kpn2I TCCGGA 1 cut(s) 1086
Kzo9I GATC 4 cut(s) 49, 423, 880, 920
LmnI GCTCC 2 cut(s) 151, 405
Lsp1109I GCAGC 4 cut(s) 150, 336, 548, 582
LweI GCATC 1 cut(s) 271
MaeI CTAG 4 cut(s) 126, 473, 635, 875
MaeII ACGT 1 cut(s) 78
MaeIII GTNAC 2 cut(s) 578, 637
MalI GATC 4 cut(s) 51, 425, 882, 922
MboI GATC 4 cut(s) 49, 423, 880, 920
MboII GAAGA 5 cut(s) 3, 433, 875, 1109, 1118
MfeI CAATTG 1 cut(s) 681
MflI RGATCY 3 cut(s) 423, 880, 920
MhlI GDGCHC 2 cut(s) 143, 485
MluCI AATT 4 cut(s) 101, 245, 681, 1059
MlyI GAGTC 1 cut(s) 1084
MroI TCCGGA 1 cut(s) 1086
MseI TTAA 2 cut(s) 84, 672
MslI CAYNNNNRTG 1 cut(s) 587
MspI CCGG 4 cut(s) 278, 628, 642, 1087
MspR9I CCNGG 6 cut(s) 342, 363, 629, 643, 702, 987
MunI CAATTG 1 cut(s) 681
Mva1269I GAATGC 1 cut(s) 843
MvaI CCWGG 4 cut(s) 342, 363, 702, 987
MwoI GCNNNNNNNGC 7 cut(s) 160, 283, 292, 346, 406, 545, 976
NciI CCSGG 2 cut(s) 629, 643
NdeII GATC 4 cut(s) 49, 423, 880, 920
NlaIII CATG 5 cut(s) 270, 441, 521, 592, 1013
NmuCI GTSAC 2 cut(s) 578, 637
NspI RCATGY 3 cut(s) 441, 521, 1013
NspV TTCGAA 1 cut(s) 218
PaeI GCATGC 3 cut(s) 441, 521, 1013
PaeR7I CTCGAG 2 cut(s) 296, 1092
PceI AGGCCT 1 cut(s) 970
PctI GAATGC 1 cut(s) 843
PfeI GAWTC 6 cut(s) 95, 171, 215, 226, 446, 814
PfoI TCCNGGA 1 cut(s) 985
PkrI GCNGC 5 cut(s) 165, 351, 411, 538, 597
PleI GAGTC 1 cut(s) 1084
PpsI GAGTC 1 cut(s) 1084
PsiI TTATAA 1 cut(s) 1002
Psp124BI GAGCTC 1 cut(s) 143
Psp6I CCWGG 4 cut(s) 340, 361, 700, 985
PspGI CCWGG 4 cut(s) 340, 361, 700, 985
PspPI GGNCC 1 cut(s) 45
PspXI VCTCGAGB 1 cut(s) 296
PstI CTGCAG 1 cut(s) 168
PstNI CAGNNNCTG 1 cut(s) 461
PsuI RGATCY 3 cut(s) 423, 880, 920
RsaI GTAC 1 cut(s) 804
RsaNI GTAC 1 cut(s) 803
RseI CAYNNNNRTG 1 cut(s) 587
SacI GAGCTC 1 cut(s) 143
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 2 cut(s) 84, 672
SatI GCNGC 5 cut(s) 164, 350, 410, 537, 596
Sau3AI GATC 4 cut(s) 49, 423, 880, 920
Sau96I GGNCC 1 cut(s) 45
SchI GAGTC 1 cut(s) 1084
ScrFI CCNGG 6 cut(s) 342, 363, 629, 643, 702, 987
SduI GDGCHC 2 cut(s) 143, 485
SfaNI GCATC 1 cut(s) 271
SfcI CTRYAG 1 cut(s) 164
Sfr274I CTCGAG 2 cut(s) 296, 1092
SfuI TTCGAA 1 cut(s) 218
SlaI CTCGAG 2 cut(s) 296, 1092
SmiMI CAYNNNNRTG 1 cut(s) 587
SmlI CTYRAG 3 cut(s) 296, 770, 1092
SmoI CTYRAG 3 cut(s) 296, 770, 1092
SphI GCATGC 3 cut(s) 441, 521, 1013
Sse9I AATT 4 cut(s) 101, 245, 681, 1059
SseBI AGGCCT 1 cut(s) 970
SsiI CCGC 2 cut(s) 345, 409
SspMI CTAG 4 cut(s) 126, 473, 635, 875
SstI GAGCTC 1 cut(s) 143
StuI AGGCCT 1 cut(s) 970
StyD4I CCNGG 6 cut(s) 340, 361, 627, 641, 700, 985
StyI CCWWGG 1 cut(s) 472
TaaI ACNGT 4 cut(s) 193, 388, 578, 691
TaiI ACGT 1 cut(s) 81
TaqI TCGA 5 cut(s) 5, 218, 297, 1093, 1104
TasI AATT 4 cut(s) 101, 245, 681, 1059
TauI GCSGC 1 cut(s) 412
TfiI GAWTC 6 cut(s) 95, 171, 215, 226, 446, 814
Tru1I TTAA 2 cut(s) 84, 672
Tru9I TTAA 2 cut(s) 84, 672
TscAI CASTG 2 cut(s) 114, 583
TseFI GTSAC 2 cut(s) 578, 637
TseI GCWGC 4 cut(s) 163, 349, 536, 595
Tsp45I GTSAC 2 cut(s) 578, 637
TspDTI ATGAA 5 cut(s) 455, 605, 836, 847, 951
TspGWI ACGGA 4 cut(s) 138, 195, 397, 969
TspRI CASTG 2 cut(s) 114, 583
XceI RCATGY 3 cut(s) 441, 521, 1013
XhoI CTCGAG 2 cut(s) 296, 1092
XmaJI CCTAGG 1 cut(s) 472
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 4 cut(s) 126, 473, 635, 875
ZraI GACGTC 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.