FvH4_7g25350

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003416
Physical Location & Seq
Reverse (-)
19379143 .. 19382440
3298 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g25350.t1

Sequence Viewer

Length: 2331 bp
ATGGATAGCCGGAACCACATTTCCGACACGCTTTGTGGTTGCCTGATTAGTAAAGGGCACCGCAATTCGCATAAGAGGAGAGAAAAATGGAAGAGTGGTAGTATACATTTAAACAATTCGTTGATCTTGCTTCCCCTGTTGTTCTTGAGTGCATTGATCTCACCGGTTAGCGGTGAGACTTGGGACGGCGTTATAGTCACAGCAGCCGACTACCAATCCCTGCAGGCGTTCAAGCACGAGCTGGATGACCCTAAGGGGTTTCTGAAGAGCTGGAACGACAGCGGCTACGGTGCCTGCTCCGGCGGCTGGGCTGGGATCAAGTGTGCTCAAGGGCAGGTCATTGTGATTCAGCTTCCTTGGAAGGGCTTAGGTGGCAGAATCACTGGGAAGATTGGACAGCTTCAAGGGTTGAGGAAGCTTAGCCTCCATGATAATCACATTGGGGGGTCAATTCCTGCTTCATTGGGCTTGCTTCCAAGCCTTAGAGGTGTGCAGTTGTTTAATAATATGCTTTCAGGTTCAATCCCACCTTCATTGGGTTTTAGTCCTCTGCTTCAGAACCTAGACCTTACTAATAACTCTCTCACTGGGTCAATCCCTGATAGTCTTGCTAACTCTACCAAGCTTTATAGGGTTGCTTTGAGCTTCAATTCGTTATCTGGGTCTTTCCCTATTAGTCTTACTCACTCACCTTCCCTGACTTTCCTTTTGATTAGTCACAATAATCTATCAGGAAACATACCAAATGAAATTGGGTCTCTTTCGAATCTTAGGATACTAGATTTGTCTAGCAATGCCATCCATGGAAGCTTGCCTGCTAGTATTGGTGCATTGCAAAACCTCTCTATTCTCAACTTGAGCAGTAACCAAATTCAGGGTCCAATCCCAACCACTCTGGGAAACATTTCCACTCTTGCACAGCTTGACTTGTCACTAAACAATCTCAGTGGTGCAATTCCGGCTGCTCTAGCTGATCTTCCGCATCTTGGTTTCCTTAATGTCTCTTACAACAACCTCACTGGTTCTGTCCCAACTCTTCTCTCCCAGAAGTTCAACGAAAGCTCTTTCGTTGGGAACATGCAGCTATGTGGGTACAGTGCTTTAACCACATGCCCTTCTGAAGCACCTTCCCAAAGTGTAGCTCCAGCACCTGAGGTAGCAAAACGTCGTCGACGCAGACTTAGCACCAAAGACAGAATTCTCATTGCAGCAGCAGTACTTCTAGTAGTTTTATTTTTACTCTGTTGCATACTGCTTTGCTGTTTGATCAGAAAAAGAGCTGCATCCAAATCAAAGGATGGCGGGGGAGCTGGCGCCGGTGCTGCTAGAACCGAAAAGGGCATTGCAGCTGGTGGGGGTGAAATTGAGTCTGGTGGAGAGGCTGGAGGAAAACTAGTCCATTTTGATGGGCCGATGGCATTTACAGCAGATGATCTTTTGTGTGCAACAGCAGAGATTATGGGGAAAAGCACCTTTGGGACAGTCTACAAGGCCACACTGGAGGATGGGAGTGAAGTTGCAGTGAAGAGATTGAGAGAAAAGATTACTAAGAATCAAAGAGAATTCGAAACAGAAGTGAATATCATTGGGAAAATCAGACATCCAAACCTCTTGGCTTTGAGGGCTTATTACTTGGGACCTAAAGGAGAAAAGCTTCTGGTTTTCGACTATATGCCTAAAGGAAGTCTGGCTGCATTTCTCCATGCTCGCGGACCTGATACACCTATCGATTGGCCTACAAGAATGAGAATTGCAAAGGGCATGGCACGAGGCTTGTCCTATCTCCACGCCAATGAGAACATCATACATGGGAACCTTACATCTAGCAATGTTCTACTTGATGAGCAAACTGATTCTAAGATTGCAGATTATGGTCTGTCTAAGCTTATGACGGCTGCTGCCAATTCAAATGTGATTGCAACAGCTGGGGCACTAGGGTACCGAGCACCCGAGCTCTCAAAGCTGAAGAAGGCCAACACGAAAACTGATGTGTACAGCCTTGGTGTGATCATATTGGAACTCCTAACAGGAAAGTCTCCCGGAGAGCCAATGAATGGTTTCGATTTGCCTCAGTGGGTTGCCTCCATTGTCAAAGAGGAGTGGACGAATGAAGTTTTTGACCTGGAATTGATGAGGGACGCGTCTGTCATCGGGGATGAGTTGCTAAACACATTGAAACTGGCTTTGCATTGTGTTGATCCTTCCCCATCAGCAAGGCCAGAAGTACAACAGGTTCTGCAGCAACTCGAAGAAATTAGACCCGAAACAGCTGCCAGTTCAGCTGATGATGGAGCTGCAGAAGCGTCAATCCCTTCAACAACAAGCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

777

Amino Acids

82.65

Weight (kDa)

7.91

Isoelectric Point (pI)

38.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 69 - 108 4e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 233 - 339 3.1e-10 Leucine-rich repeat region
LRR_8 PF13855 280 - 339 1.8e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 484 - 749 2.7e-42 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 484 - 748 2.8e-39 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2144
Acc36I ACCTGC 1 cut(s) 325
Acc65I GGTACC 1 cut(s) 1938
AccB1I GGYRCC 4 cut(s) 57, 290, 1313, 1938
AccB7I CCANNNNNTGG 1 cut(s) 2054
AccI GTMKAC 3 cut(s) 103, 1171, 1485
AccII CGCG 2 cut(s) 1710, 2141
AciI CCGC 7 cut(s) 61, 171, 282, 303, 980, 1302, 1710
AclWI GGATC 2 cut(s) 323, 2192
AcsI RAATTY 3 cut(s) 870, 1197, 1562
AcuI CTGAAG 4 cut(s) 284, 539, 1140, 1985
AcyI GRCGYC 1 cut(s) 1314
AfaI GTAC 5 cut(s) 1094, 1218, 1940, 1994, 2226
AfiI CCNNNNNNNGG 7 cut(s) 170, 306, 362, 536, 874, 986, 2054
AflIII ACRYGT 1 cut(s) 2139
AgeI ACCGGT 1 cut(s) 163
AgsI TTSAA 8 cut(s) 232, 404, 522, 649, 1054, 1908, 2176, 2316
AhlI ACTAGT 1 cut(s) 1393
AjnI CCWGG 1 cut(s) 2121
AloI GAACNNNNNNTCC 1 cut(s) 37
Alw21I GWGCWC 3 cut(s) 328, 1948, 1956
Alw26I GTCTC 4 cut(s) 170, 762, 1006, 2040
AlwI GGATC 2 cut(s) 323, 2192
AlwNI CAGNNNCTG 2 cut(s) 1151, 2236
Ama87I CYCGRG 1 cut(s) 1949
AoxI GGCC 5 cut(s) 1409, 1491, 1733, 1971, 2216
ApoI RAATTY 3 cut(s) 870, 1197, 1562
ArsI GACNNNNNNTTYG 4 cut(s) 691, 723, 862, 894
AsiGI ACCGGT 1 cut(s) 163
Asp700I GAANNNNTTC 3 cut(s) 904, 1653, 2057
Asp718I GGTACC 1 cut(s) 1938
AspLEI GCGC 1 cut(s) 1316
AspS9I GGNCC 4 cut(s) 878, 1409, 1637, 1712
AsuC2I CCSGG 1 cut(s) 2040
AsuHPI GGTGA 4 cut(s) 153, 185, 681, 1370
AsuII TTCGAA 2 cut(s) 764, 1566
AvaI CYCGRG 1 cut(s) 1949
AvaII GGWCC 3 cut(s) 878, 1637, 1712
AxyI CCTNAGG 2 cut(s) 252, 1152
BaeGI GKGCMC 2 cut(s) 60, 1933
BaeI ACNNNNGTAYC 2 cut(s) 1922, 1955
BanI GGYRCC 4 cut(s) 57, 290, 1313, 1938
BanII GRGCYC 1 cut(s) 1956
BauI CACGAG 2 cut(s) 236, 1767
Bbv12I GWGCWC 3 cut(s) 328, 1948, 1956
BccI CCATC 7 cut(s) 806, 1292, 1400, 1408, 1499, 2215, 2282
BceAI ACGGC 2 cut(s) 202, 1908
BcgI CGANNNNNNTGC 2 cut(s) 2252, 2286
BciT130I CCWGG 1 cut(s) 2123
BciVI GTATCC 1 cut(s) 768
BclI TGATCA 2 cut(s) 1266, 2007
BcnI CCSGG 1 cut(s) 2040
BcoDI GTCTC 4 cut(s) 170, 762, 1006, 2040
BcuI ACTAGT 1 cut(s) 1393
BfmI CTRYAG 3 cut(s) 221, 2237, 2295
BfoI RGCGCY 1 cut(s) 1317
BfuAI ACCTGC 1 cut(s) 325
BfuI GTATCC 1 cut(s) 768
BlpI GCTNAGC 1 cut(s) 419
BmcAI AGTACT 1 cut(s) 1218
Bme1390I CCNGG 2 cut(s) 2040, 2123
Bme18I GGWCC 3 cut(s) 878, 1637, 1712
BmeT110I CYCGRG 1 cut(s) 1949
BmgT120I GGNCC 4 cut(s) 878, 1409, 1637, 1712
BmiI GGNNCC 8 cut(s) 14, 59, 292, 879, 1315, 1638, 1814, 1940
BmrFI CCNGG 2 cut(s) 2040, 2123
BmrI ACTGGG 2 cut(s) 393, 597
BmsI GCATC 2 cut(s) 991, 1292
BmuI ACTGGG 2 cut(s) 393, 597
BpmI CTGGAG 3 cut(s) 1128, 1404, 1520
Bpu10I CCTNAGC 1 cut(s) 367
Bpu1102I GCTNAGC 1 cut(s) 419
Bpu14I TTCGAA 2 cut(s) 764, 1566
BpuEI CTTGAG 3 cut(s) 166, 312, 877
BpuMI CCSGG 1 cut(s) 2040
Bsa29I ATCGAT 1 cut(s) 1728
BsaHI GRCGYC 1 cut(s) 1314
BsaI GGTCTC 1 cut(s) 762
BsaJI CCNNGG 3 cut(s) 356, 802, 1999
BsaWI WCCGGW 1 cut(s) 163
Bsc4I CCNNNNNNNGG 7 cut(s) 170, 306, 362, 536, 874, 986, 2054
Bse118I RCCGGY 2 cut(s) 163, 1316
Bse1I ACTGG 6 cut(s) 388, 592, 1024, 1503, 2184, 2274
Bse21I CCTNAGG 2 cut(s) 252, 1152
Bse3DI GCAATG 5 cut(s) 799, 830, 1203, 1341, 1834
BseBI CCWGG 1 cut(s) 2123
BseCI ATCGAT 1 cut(s) 1728
BseDI CCNNGG 3 cut(s) 356, 802, 1999
BseGI GGATG 7 cut(s) 250, 798, 1283, 1303, 1510, 1600, 2161
BseLI CCNNNNNNNGG 7 cut(s) 170, 306, 362, 536, 874, 986, 2054
BseMI GCAATG 5 cut(s) 799, 830, 1203, 1341, 1834
BseMII CTCAG 3 cut(s) 958, 1143, 2084
BseNI ACTGG 6 cut(s) 388, 592, 1024, 1503, 2184, 2274
BseRI GAGGAG 2 cut(s) 91, 2111
BseSI GKGCMC 2 cut(s) 60, 1933
BseYI CCCAGC 3 cut(s) 306, 311, 1925
BsgI GTGCAG 1 cut(s) 512
Bsh1236I CGCG 2 cut(s) 1710, 2141
BshFI GGCC 5 cut(s) 1411, 1493, 1735, 1973, 2218
BshNI GGYRCC 4 cut(s) 57, 290, 1313, 1938
BshTI ACCGGT 1 cut(s) 163
BshVI ATCGAT 1 cut(s) 1728
BsiHKAI GWGCWC 3 cut(s) 328, 1948, 1956
BsiHKCI CYCGRG 1 cut(s) 1949
BsiSI CCGG 6 cut(s) 10, 164, 300, 959, 1317, 2040
BslFI GGGAC 5 cut(s) 197, 1013, 1492, 1650, 2150
BslI CCNNNNNNNGG 7 cut(s) 170, 306, 362, 536, 874, 986, 2054
BsmAI GTCTC 4 cut(s) 170, 762, 1006, 2040
BsmFI GGGAC 5 cut(s) 197, 1013, 1492, 1650, 2150
BsnI GGCC 5 cut(s) 1411, 1493, 1735, 1973, 2218
Bso31I GGTCTC 1 cut(s) 762
BsoBI CYCGRG 1 cut(s) 1949
Bsp119I TTCGAA 2 cut(s) 764, 1566
Bsp1286I GDGCHC 5 cut(s) 60, 328, 1933, 1948, 1956
Bsp1407I TGTACA 1 cut(s) 1992
Bsp143I GATC 8 cut(s) 123, 156, 315, 973, 1266, 1432, 2007, 2197
Bsp1720I GCTNAGC 1 cut(s) 419
Bsp19I CCATGG 1 cut(s) 802
BspACI CCGC 7 cut(s) 61, 171, 282, 303, 980, 1302, 1710
BspANI GGCC 5 cut(s) 1411, 1493, 1735, 1973, 2218
BspCNI CTCAG 3 cut(s) 957, 1144, 2083
BspDI ATCGAT 1 cut(s) 1728
BspFNI CGCG 2 cut(s) 1710, 2141
BspLI GGNNCC 8 cut(s) 14, 59, 292, 879, 1315, 1638, 1814, 1940
BspMAI CTGCAG 3 cut(s) 225, 2241, 2299
BspMI ACCTGC 1 cut(s) 325
BspPI GGATC 2 cut(s) 323, 2192
BspQI GCTCTTC 1 cut(s) 260
BspT104I TTCGAA 2 cut(s) 764, 1566
BspT107I GGYRCC 4 cut(s) 57, 290, 1313, 1938
BspTNI GGTCTC 1 cut(s) 762
BsrDI GCAATG 5 cut(s) 799, 830, 1203, 1341, 1834
BsrFI RCCGGY 2 cut(s) 163, 1316
BsrGI TGTACA 1 cut(s) 1992
BsrI ACTGG 6 cut(s) 388, 592, 1024, 1503, 2184, 2274
BssAI RCCGGY 2 cut(s) 163, 1316
BssECI CCNNGG 3 cut(s) 356, 802, 1999
BssMI GATC 8 cut(s) 123, 156, 315, 973, 1266, 1432, 2007, 2197
BssNAI GTATAC 1 cut(s) 104
BssNI GRCGYC 1 cut(s) 1314
BssSI CACGAG 2 cut(s) 236, 1767
BssT1I CCWWGG 3 cut(s) 356, 802, 1999
Bst1107I GTATAC 1 cut(s) 104
Bst2BI CACGAG 2 cut(s) 236, 1767
Bst2UI CCWGG 1 cut(s) 2123
Bst4CI ACNGT 3 cut(s) 290, 1097, 1483
Bst6I CTCTTC 4 cut(s) 86, 260, 1041, 1520
BstACI GRCGYC 1 cut(s) 1314
BstAUI TGTACA 1 cut(s) 1992
BstBI TTCGAA 2 cut(s) 764, 1566
BstC8I GCNNGC 7 cut(s) 225, 295, 470, 812, 816, 1312, 1708
BstDSI CCRYGG 1 cut(s) 802
BstF5I GGATG 7 cut(s) 250, 798, 1283, 1303, 1510, 1600, 2161
BstFNI CGCG 2 cut(s) 1710, 2141
BstH2I RGCGCY 1 cut(s) 1317
BstHHI GCGC 1 cut(s) 1316
BstKTI GATC 8 cut(s) 126, 159, 318, 976, 1269, 1435, 2010, 2200
BstMAI GTCTC 4 cut(s) 170, 762, 1006, 2040
BstMBI GATC 8 cut(s) 123, 156, 315, 973, 1266, 1432, 2007, 2197
BstNI CCWGG 1 cut(s) 2123
BstNSI RCATGY 2 cut(s) 1081, 1113
BstSCI CCNGG 2 cut(s) 2038, 2121
BstSFI CTRYAG 3 cut(s) 221, 2237, 2295
BstSLI GKGCMC 2 cut(s) 60, 1933
BstUI CGCG 2 cut(s) 1710, 2141
BstXI CCANNNNNNTGG 1 cut(s) 1406
BstZ17I GTATAC 1 cut(s) 104
Bsu15I ATCGAT 1 cut(s) 1728
Bsu36I CCTNAGG 2 cut(s) 252, 1152
BsuI GTATCC 1 cut(s) 768
BsuRI GGCC 5 cut(s) 1411, 1493, 1735, 1973, 2218
BsuTUI ATCGAT 1 cut(s) 1728
BtgI CCRYGG 1 cut(s) 802
BtsCI GGATG 7 cut(s) 250, 798, 1283, 1303, 1510, 1600, 2161
BtsI GCAGTG 1 cut(s) 1527
BtsIMutI CAGTG 8 cut(s) 381, 585, 952, 1017, 1102, 1496, 1527, 2078
BveI ACCTGC 1 cut(s) 325
Cac8I GCNNGC 7 cut(s) 225, 295, 470, 812, 816, 1312, 1708
CaiI CAGNNNCTG 2 cut(s) 1151, 2236
CfoI GCGC 1 cut(s) 1316
Cfr10I RCCGGY 2 cut(s) 163, 1316
Cfr13I GGNCC 4 cut(s) 878, 1409, 1637, 1712
ClaI ATCGAT 1 cut(s) 1728
CseI GACGC 4 cut(s) 1182, 2130, 2147, 2292
Csp6I GTAC 5 cut(s) 1093, 1217, 1939, 1993, 2225
CspAI ACCGGT 1 cut(s) 163
CspCI CAANNNNNGTGG 4 cut(s) 516, 551, 928, 963
CviAII CATG 7 cut(s) 428, 803, 1078, 1110, 1703, 1762, 1808
CviQI GTAC 5 cut(s) 1093, 1217, 1939, 1993, 2225
DinI GGCGCC 1 cut(s) 1315
DpnI GATC 8 cut(s) 125, 158, 317, 975, 1268, 1434, 2009, 2199
DpnII GATC 8 cut(s) 123, 156, 315, 973, 1266, 1432, 2007, 2197
DraI TTTAAA 1 cut(s) 111
DrdI GACNNNNNNGTC 1 cut(s) 2144
DseDI GACNNNNNNGTC 1 cut(s) 2144
Eam1104I CTCTTC 4 cut(s) 86, 260, 1041, 1520
EarI CTCTTC 4 cut(s) 86, 260, 1041, 1520
Ecl136II GAGCTC 1 cut(s) 1954
Eco130I CCWWGG 3 cut(s) 356, 802, 1999
Eco24I GRGCYC 1 cut(s) 1956
Eco31I GGTCTC 1 cut(s) 762
Eco47I GGWCC 3 cut(s) 878, 1637, 1712
Eco53kI GAGCTC 1 cut(s) 1954
Eco57I CTGAAG 4 cut(s) 284, 539, 1140, 1985
Eco81I CCTNAGG 2 cut(s) 252, 1152
Eco88I CYCGRG 1 cut(s) 1949
EcoICRI GAGCTC 1 cut(s) 1954
EcoO109I RGGNCCY 1 cut(s) 1637
EcoRI GAATTC 2 cut(s) 1197, 1562
EcoRII CCWGG 1 cut(s) 2121
EcoT14I CCWWGG 3 cut(s) 356, 802, 1999
EcoT38I GRGCYC 1 cut(s) 1956
EgeI GGCGCC 1 cut(s) 1315
EheI GGCGCC 1 cut(s) 1315
ErhI CCWWGG 3 cut(s) 356, 802, 1999
FaeI CATG 7 cut(s) 431, 806, 1081, 1113, 1706, 1765, 1811
FaqI GGGAC 5 cut(s) 197, 1013, 1492, 1650, 2150
FatI CATG 7 cut(s) 427, 802, 1077, 1109, 1702, 1761, 1807
FauI CCCGC 1 cut(s) 1295
FbaI TGATCA 2 cut(s) 1266, 2007
FblI GTMKAC 3 cut(s) 103, 1171, 1485
FokI GGATG 7 cut(s) 257, 785, 1270, 1310, 1517, 1587, 2168
FriOI GRGCYC 1 cut(s) 1956
GlaI GCGC 1 cut(s) 1315
GsaI CCCAGC 3 cut(s) 310, 315, 1929
GsuI CTGGAG 3 cut(s) 1128, 1404, 1520
HaeII RGCGCY 1 cut(s) 1317
HaeIII GGCC 5 cut(s) 1411, 1493, 1735, 1973, 2218
HapII CCGG 6 cut(s) 10, 164, 300, 959, 1317, 2040
HgaI GACGC 4 cut(s) 1182, 2130, 2147, 2292
HhaI GCGC 1 cut(s) 1316
Hin1I GRCGYC 1 cut(s) 1314
Hin1II CATG 7 cut(s) 431, 806, 1081, 1113, 1706, 1765, 1811
Hin6I GCGC 1 cut(s) 1314
HinP1I GCGC 1 cut(s) 1314
HincII GTYRAC 1 cut(s) 1172
HindII GTYRAC 1 cut(s) 1172
HindIII AAGCTT 5 cut(s) 416, 623, 808, 1652, 1883
HinfI GANTC 6 cut(s) 346, 378, 766, 1367, 1552, 1853
HpaII CCGG 6 cut(s) 10, 164, 300, 959, 1317, 2040
HphI GGTGA 4 cut(s) 153, 185, 681, 1370
Hpy166II GTNNAC 5 cut(s) 104, 1172, 1486, 1993, 2103
Hpy188I TCNGA 6 cut(s) 25, 264, 558, 1120, 1271, 1598
Hpy188III TCNNGA 2 cut(s) 145, 732
Hpy8I GTNNAC 5 cut(s) 104, 1172, 1486, 1993, 2103
Hpy99I CGWCG 3 cut(s) 1170, 1173, 1176
HpyAV CCTTC 8 cut(s) 355, 540, 702, 1125, 1137, 1963, 2211, 2322
HpyCH4III ACNGT 3 cut(s) 290, 1097, 1483
HpyCH4IV ACGT 1 cut(s) 1165
HpySE526I ACGT 1 cut(s) 1165
Hsp92I GRCGYC 1 cut(s) 1314
Hsp92II CATG 7 cut(s) 431, 806, 1081, 1113, 1706, 1765, 1811
HspAI GCGC 1 cut(s) 1314
KasI GGCGCC 1 cut(s) 1313
KpnI GGTACC 1 cut(s) 1942
Ksp22I TGATCA 2 cut(s) 1266, 2007
Kzo9I GATC 8 cut(s) 123, 156, 315, 973, 1266, 1432, 2007, 2197
LguI GCTCTTC 1 cut(s) 260
LmnI GCTCC 4 cut(s) 302, 1147, 1307, 2291
LweI GCATC 2 cut(s) 991, 1292
MaeII ACGT 1 cut(s) 1165
MaeIII GTNAC 4 cut(s) 196, 716, 863, 930
MalI GATC 8 cut(s) 125, 158, 317, 975, 1268, 1434, 2009, 2199
MboI GATC 8 cut(s) 123, 156, 315, 973, 1266, 1432, 2007, 2197
MboII GAAGA 8 cut(s) 103, 277, 400, 968, 1028, 1537, 1978, 2261
MhlI GDGCHC 5 cut(s) 60, 328, 1933, 1948, 1956
MluI ACGCGT 1 cut(s) 2139
Mly113I GGCGCC 1 cut(s) 1314
MlyI GAGTC 1 cut(s) 1376
MmeI TCCRAC 1 cut(s) 48
MroXI GAANNNNTTC 3 cut(s) 904, 1653, 2057
MseI TTAA 4 cut(s) 110, 501, 996, 1103
MslI CAYNNNNRTG 2 cut(s) 1404, 1791
MspA1I CMGCKG 5 cut(s) 282, 1349, 1925, 2270, 2282
MspI CCGG 6 cut(s) 10, 164, 300, 959, 1317, 2040
MspR9I CCNGG 2 cut(s) 2040, 2123
MvaI CCWGG 1 cut(s) 2123
MvnI CGCG 2 cut(s) 1710, 2141
NarI GGCGCC 1 cut(s) 1314
NciI CCSGG 1 cut(s) 2040
NcoI CCATGG 1 cut(s) 802
NdeII GATC 8 cut(s) 123, 156, 315, 973, 1266, 1432, 2007, 2197
NlaIII CATG 7 cut(s) 431, 806, 1081, 1113, 1706, 1765, 1811
NlaIV GGNNCC 8 cut(s) 14, 59, 292, 879, 1315, 1638, 1814, 1940
NmuCI GTSAC 3 cut(s) 196, 716, 930
NspI RCATGY 2 cut(s) 1081, 1113
NspV TTCGAA 2 cut(s) 764, 1566
PciSI GCTCTTC 1 cut(s) 260
PdmI GAANNNNTTC 3 cut(s) 904, 1653, 2057
PfeI GAWTC 5 cut(s) 346, 378, 766, 1552, 1853
PflMI CCANNNNNTGG 1 cut(s) 2054
PfoI TCCNGGA 1 cut(s) 2038
PinAI ACCGGT 1 cut(s) 163
PleI GAGTC 1 cut(s) 1375
PluTI GGCGCC 1 cut(s) 1317
PpsI GAGTC 1 cut(s) 1375
PpuMI RGGWCCY 1 cut(s) 1637
Psp124BI GAGCTC 1 cut(s) 1956
Psp5II RGGWCCY 1 cut(s) 1637
Psp6I CCWGG 1 cut(s) 2121
PspFI CCCAGC 3 cut(s) 306, 311, 1925
PspGI CCWGG 1 cut(s) 2121
PspN4I GGNNCC 8 cut(s) 14, 59, 292, 879, 1315, 1638, 1814, 1940
PspPI GGNCC 4 cut(s) 878, 1409, 1637, 1712
PspPPI RGGWCCY 1 cut(s) 1637
PsrI GAACNNNNNNTAC 2 cut(s) 2217, 2249
PstI CTGCAG 3 cut(s) 225, 2241, 2299
PstNI CAGNNNCTG 2 cut(s) 1151, 2236
PvuII CAGCTG 4 cut(s) 1349, 1925, 2270, 2282
RsaI GTAC 5 cut(s) 1094, 1218, 1940, 1994, 2226
RsaNI GTAC 5 cut(s) 1093, 1217, 1939, 1993, 2225
RseI CAYNNNNRTG 2 cut(s) 1404, 1791
SacI GAGCTC 1 cut(s) 1956
SalI GTCGAC 1 cut(s) 1170
SapI GCTCTTC 1 cut(s) 260
SaqAI TTAA 4 cut(s) 110, 501, 996, 1103
Sau3AI GATC 8 cut(s) 123, 156, 315, 973, 1266, 1432, 2007, 2197
Sau96I GGNCC 4 cut(s) 878, 1409, 1637, 1712
SbfI CCTGCAGG 1 cut(s) 225
ScaI AGTACT 1 cut(s) 1218
SchI GAGTC 1 cut(s) 1376
ScrFI CCNGG 2 cut(s) 2040, 2123
SdaI CCTGCAGG 1 cut(s) 225
SduI GDGCHC 5 cut(s) 60, 328, 1933, 1948, 1956
SfaNI GCATC 2 cut(s) 991, 1292
SfcI CTRYAG 3 cut(s) 221, 2237, 2295
SfoI GGCGCC 1 cut(s) 1315
SfuI TTCGAA 2 cut(s) 764, 1566
SgrAI CRCCGGYG 1 cut(s) 1316
SgrDI CGTCGACG 1 cut(s) 1170
SinI GGWCC 3 cut(s) 878, 1637, 1712
SmiMI CAYNNNNRTG 2 cut(s) 1404, 1791
SmlI CTYRAG 3 cut(s) 145, 327, 856
SmoI CTYRAG 3 cut(s) 145, 327, 856
SpeI ACTAGT 1 cut(s) 1393
Sse8387I CCTGCAGG 1 cut(s) 225
SsiI CCGC 7 cut(s) 61, 171, 282, 303, 980, 1302, 1710
SspDI GGCGCC 1 cut(s) 1313
SstI GAGCTC 1 cut(s) 1956
StyD4I CCNGG 2 cut(s) 2038, 2121
StyI CCWWGG 3 cut(s) 356, 802, 1999
TaaI ACNGT 3 cut(s) 290, 1097, 1483
TaiI ACGT 1 cut(s) 1168
TaqI TCGA 7 cut(s) 764, 1171, 1566, 1665, 1728, 2061, 2247
TatI WGTACW 3 cut(s) 1216, 1992, 2224
TauI GCSGC 2 cut(s) 285, 306
TfiI GAWTC 5 cut(s) 346, 378, 766, 1552, 1853
Tru1I TTAA 4 cut(s) 110, 501, 996, 1103
Tru9I TTAA 4 cut(s) 110, 501, 996, 1103
TscAI CASTG 8 cut(s) 388, 592, 952, 1024, 1102, 1503, 1527, 2078
TseFI GTSAC 3 cut(s) 196, 716, 930
Tsp45I GTSAC 3 cut(s) 196, 716, 930
TspDTI ATGAA 5 cut(s) 450, 522, 762, 2066, 2124
TspRI CASTG 8 cut(s) 388, 592, 952, 1024, 1102, 1503, 1527, 2078
Van91I CCANNNNNTGG 1 cut(s) 2054
VpaK11BI GGWCC 3 cut(s) 878, 1637, 1712
XapI RAATTY 3 cut(s) 870, 1197, 1562
XceI RCATGY 2 cut(s) 1081, 1113
XmiI GTMKAC 3 cut(s) 103, 1171, 1485
XmnI GAANNNNTTC 3 cut(s) 904, 1653, 2057
ZrmI AGTACT 1 cut(s) 1218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.