pycom12g16950
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0003416
Physical Location & Seq
Reverse (-)
19232701 .. 19235304
2604 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g16950.1

Sequence Viewer

Length: 2487 bp
ATGTTTATGGAATATAAGTCCTTCTTCATCCATGCCTTGTTCTTTGTTCATCTTTTGGTTGTCACTTACCGGCCCGTTTCGGGCCAGCGAGGGAATGATGGAATGATTGTGACTCAGTCCGATTACCAAGCTCTTCGAGCTTTCAAGCGTGAGCTCATTGATTTCACCGGCGTTCTGCGCAGCTGGAATGATAGCGGACGTGGAGCCTGCTCAGGCGCGTGGGCAGGGATTAAGTGTGTGAACGGACAGGTTATTGCAATCCAGCTTCCGTGGAGGAGACTCGGGGGCCGAATATCTGAAAAGATTGGGCAGCTGCAAGCGCTTCGGAAGCTCAGCCTGCACGACAATGTCTTGGCTGGCCCTGTCCCCTTGTCTCTCGGCTTCCTTCCCAATCTCAGAGGAGTTTACCTCTTCAACAACCGGCTTTCGGGGTCTGTCCCGCCTTCTATTGGTAACTGTCCTCTTCTCCAAACTCTTGATTTAAGCAACAATTCGCTTACTGGCACGATTCCTTCTAGTCTTGCAAATCCTAAGAAGTTGTATAGACTTAATCTGAGCTTCAACTCGCTTTCGGGTTCCATCCCAACAAGTCTCACCAAATCCCGTTCTCTCACCATCCTTGTACTCCAACACAACAACCTATCTGGTTCCATACCAGGCACTTGGGATGCTGGAAATCGAAATCTAAGTTACCAACTTATGATCCTGACCCTTGATCATAATCTGATTTCCGGAACTATTCCTAGTTCTCTGAGCAAATTGGGTTTTCTTCAAGAGATTTATCTGGACGACAACCAGATTTCCGGGACCATTCCTGAAGAAATAGGGGAGCTCACAAGGCTCCAGAAGCTAGACTTATCCAACAATGCCATCAATGGGAGCTTTCCTTCTAGCTTTTCCAACCTCTCCTCCCTTGTTTCGTTGAACCTCGAGGGCAACCACCTCGATAACCAAATCCCGGAAGGCCTGGAAAGGTTGCAGAACCTCTCAGTGCTCAACCTGAGGAAGAACAATTTCAGTGGCCACATTCCAGCATCTGTGGGGAATATCTCTGGAATCTACCAACTGGATTTATCGGAAAACAAATTCAGTGGTGAAATTCCTGCTTCACTTTCCAGCCTTGCCAATCTCACTTCGTTCAATGTCTCTCACAACAATCTTTCTGGCGCCGTCCCTTCTCTGCTCTCAAAAAAGTTCAATGCCAACTCTTTCGCGGGGAATCTTCAGCTGTGCGGGTACAGTGCTTCAACTCCGTGCTCTTCTCCCCCGCCTCAGATTCTTCCATCTTCTCCGCCAACGGAGCAGCCTTTGAAGAAAAAGCATCACCACAAATTTAGTACAAAGGACAAAATTCTCATAGCGGCGGGTGCCCTCCTGGCGGTTCTGCTTCTACTCTGCTGCATTTTGCTTGTTTGTTTGGTCAGGAAAAGGTCTGCTTCAAGAGGAAAGAATGGTAAAGCCGCCATGCAGGCCGCTGCTCCAGGGGGCGCTGCTAAGGCAGTTCCTGGCGGCGTTGGAGTTGAATATGGCGGTGAGGCTGGTGGGAAGCTTGTTCACTTTGATGGGCCATTTGTTTTTACAGCTGATGATCTCTTATGTGCCACTGCTGAGATAATGGGGAAGAGCACATATGGGACTGCATACAAGGCGACATTAGAGGAAGGAAACCAAGTTGCAGTAAAGAGATTGAGGGAAAAGACGGCGAAAGGTCAGAAGGAGTTTGAAATGGAGGCTGCAGCAATTGGGAAGATTCGCCACCCGAATCTCTTGGCTCTGAGGGCTTATTACTTGGGACCCAAGGGAGAGAAGCTTCTCGTCTTCGATTACATGCCTAAGGGTAGCCTTGCGTCCTTCCTTCATGCTCGAGGGCCGGAAACCATCATTGATTGGCCAACAAGGATGAACATAGTGATTGGCATCACGCGTGGACTATGCCACCTCCACAACGAGGAGAACATTGTGCATGGAAATCTTACATCCGGCAACATTCTGCTTGATGAGCAGACAAATGCTCACATTGCAGACTTTGGCCTTTCACGCCTTATGACTGCGGCCGCCAACACCAATGTGATTGCCACTGCAGGAACCCTCGGCTACAACGCACCTGAGCTCTCAAAGGCCAAGAAGGCCACAACAAAGACTGATGTGTACAGCCTTGGGGTGATCATACTGGAGCTCCTGACAGGAAAATCCCCCGGGGAACCGATGAACGGCATGGATTTGCCACAGTGGGTGGCTTCGATTGTGAAAGAGGAGTGGATAAATGAAGTTTTTGATTTGGAGATCATGAGGGATGTGCCAACCATTGGTGACCTGCTGCTTAACACATTGAAGTTGGCTCTGCATTGTGTGGATCCATCGCCGGCTTTGCGGCCGGAAGCTCAGCAAGTTCTGGAGCAGCTGGAGGAGATTAAGCCTGATCTGCCAACTGGTGGTTCTGCAGAAGAAGGAGCAGAAGTACCACCACCAGCAGGCAGCAGCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

829

Amino Acids

89.06

Weight (kDa)

8.45

Isoelectric Point (pI)

37.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 39 - 79 9.4e-08 Leucine rich repeat N-terminal domain
LRR_4 PF12799 130 - 168 2e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 155 - 214 1.4e-07 Leucine rich repeat
LRR_14 PF23598 250 - 384 1.8e-11 Leucine-rich repeat region
LRR_8 PF13855 257 - 315 6.2e-10 Leucine rich repeat
Pkinase PF00069 536 - 800 6.7e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 537 - 801 6.7e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 929
Acc16I TGCGCA 1 cut(s) 179
Acc36I ACCTGC 1 cut(s) 2322
AccB1I GGYRCC 2 cut(s) 1166, 1367
AccB7I CCANNNNNTGG 2 cut(s) 2306, 2432
AccII CGCG 3 cut(s) 218, 1214, 1924
AccIII TCCGGA 1 cut(s) 731
AclWI GGATC 3 cut(s) 697, 2348, 2361
AcoI YGGCCR 4 cut(s) 1021, 1889, 2052, 2372
AcsI RAATTY 4 cut(s) 1085, 1098, 1331, 1350
AcuI CTGAAG 2 cut(s) 837, 1208
AcyI GRCGYC 1 cut(s) 1167
AfaI GTAC 5 cut(s) 624, 1238, 1339, 2150, 2460
AfeI AGCGCT 1 cut(s) 321
AflIII ACRYGT 1 cut(s) 1922
AjiI CACGTC 1 cut(s) 200
AjnI CCWGG 5 cut(s) 655, 966, 1374, 1480, 1504
AjuI GAANNNNNNNTTGG 2 cut(s) 2419, 2451
AleI CACNNNNGTG 1 cut(s) 2066
Alw21I GWGCWC 7 cut(s) 156, 834, 996, 1259, 1628, 2112, 2178
Alw26I GTCTC 4 cut(s) 271, 378, 596, 1150
AlwI GGATC 3 cut(s) 697, 2348, 2361
AlwNI CAGNNNCTG 3 cut(s) 1037, 1505, 2484
Ama87I CYCGRG 4 cut(s) 281, 929, 1863, 2195
Aor13HI TCCGGA 1 cut(s) 731
Aor51HI AGCGCT 1 cut(s) 321
ApoI RAATTY 4 cut(s) 1085, 1098, 1331, 1350
Asp700I GAANNNNTTC 1 cut(s) 1013
AspLEI GCGC 5 cut(s) 180, 218, 322, 1169, 1490
AspS9I GGNCC 8 cut(s) 72, 82, 286, 359, 807, 1565, 1793, 1868
AsuC2I CCSGG 4 cut(s) 805, 959, 2196, 2197
AsuHPI GGTGA 8 cut(s) 157, 586, 604, 1106, 1316, 1544, 2173, 2321
AvaI CYCGRG 4 cut(s) 281, 929, 1863, 2195
AvaII GGWCC 2 cut(s) 807, 1793
AxyI CCTNAGG 2 cut(s) 1001, 1833
BaeGI GKGCMC 1 cut(s) 1372
BalI TGGCCA 2 cut(s) 1023, 1891
BamHI GGATCC 1 cut(s) 2353
BanI GGYRCC 2 cut(s) 1166, 1367
BanII GRGCYC 4 cut(s) 156, 834, 2112, 2178
BbsI GAAGAC 1 cut(s) 1810
Bbv12I GWGCWC 7 cut(s) 156, 834, 996, 1259, 1628, 2112, 2178
BccI CCATC 8 cut(s) 92, 587, 623, 878, 1291, 1556, 1886, 2365
BceAI ACGGC 3 cut(s) 1154, 1716, 2227
BcgI CGANNNNNNTGC 4 cut(s) 305, 339, 925, 959
BciT130I CCWGG 5 cut(s) 657, 968, 1376, 1482, 1506
BclI TGATCA 2 cut(s) 715, 2163
BcnI CCSGG 4 cut(s) 805, 959, 2196, 2197
BcoDI GTCTC 4 cut(s) 271, 378, 596, 1150
BfaI CTAG 4 cut(s) 516, 744, 851, 891
BfmI CTRYAG 3 cut(s) 1734, 2079, 2439
BfoI RGCGCY 3 cut(s) 323, 1170, 1491
BfuAI ACCTGC 1 cut(s) 2322
BglI GCCNNNNNGGC 3 cut(s) 1376, 1469, 2126
BlpI GCTNAGC 2 cut(s) 332, 2382
Bme1390I CCNGG 9 cut(s) 657, 805, 959, 968, 1376, 1482, 1506, 2196, 2197
Bme18I GGWCC 2 cut(s) 807, 1793
BmeT110I CYCGRG 4 cut(s) 281, 929, 1863, 2195
BmgBI CACGTC 1 cut(s) 200
BmgT120I GGNCC 8 cut(s) 72, 82, 286, 359, 807, 1565, 1793, 1868
BmrFI CCNGG 9 cut(s) 657, 805, 959, 968, 1376, 1482, 1506, 2196, 2197
BmsI GCATC 4 cut(s) 658, 1043, 1330, 1926
BpiI GAAGAC 1 cut(s) 1810
BplI GAGNNNNNCTC 2 cut(s) 393, 425
BpmI CTGGAG 5 cut(s) 827, 1464, 2192, 2414, 2423
Bpu10I CCTNAGC 3 cut(s) 211, 1494, 2106
Bpu1102I GCTNAGC 2 cut(s) 332, 2382
BpuMI CCSGG 4 cut(s) 805, 959, 2196, 2197
BsaBI GATNNNNATC 2 cut(s) 720, 1916
BsaHI GRCGYC 1 cut(s) 1167
BsaJI CCNNGG 8 cut(s) 269, 1481, 1797, 2089, 2155, 2194, 2195, 2196
BsaWI WCCGGW 1 cut(s) 731
BsaXI ACNNNNNCTCC 6 cut(s) 893, 923, 2160, 2190, 2442, 2472
Bse118I RCCGGY 4 cut(s) 69, 167, 420, 2362
Bse1I ACTGG 4 cut(s) 505, 1071, 2175, 2434
Bse21I CCTNAGG 2 cut(s) 1001, 1833
Bse3DI GCAATG 1 cut(s) 2016
Bse8I GATNNNNATC 2 cut(s) 720, 1916
BseAI TCCGGA 1 cut(s) 731
BseBI CCWGG 5 cut(s) 657, 968, 1376, 1482, 1506
BseDI CCNNGG 8 cut(s) 269, 1481, 1797, 2089, 2155, 2194, 2195, 2196
BseGI GGATG 7 cut(s) 27, 579, 615, 673, 1905, 1976, 2299
BseJI GATNNNNATC 2 cut(s) 720, 1916
BseMI GCAATG 1 cut(s) 2016
BseNI ACTGG 4 cut(s) 505, 1071, 2175, 2434
BseRI GAGGAG 6 cut(s) 289, 414, 898, 1964, 2267, 2420
BseSI GKGCMC 1 cut(s) 1372
BseX3I CGGCCG 2 cut(s) 2052, 2372
BsgI GTGCAG 1 cut(s) 323
Bsh1236I CGCG 3 cut(s) 218, 1214, 1924
Bsh1285I CGRYCG 2 cut(s) 2055, 2375
BshNI GGYRCC 2 cut(s) 1166, 1367
BsiEI CGRYCG 2 cut(s) 2055, 2375
BsiHKAI GWGCWC 7 cut(s) 156, 834, 996, 1259, 1628, 2112, 2178
BsiHKCI CYCGRG 4 cut(s) 281, 929, 1863, 2195
BslFI GGGAC 6 cut(s) 350, 422, 820, 1157, 1648, 1806
BsmAI GTCTC 4 cut(s) 271, 378, 596, 1150
BsmFI GGGAC 6 cut(s) 350, 422, 820, 1157, 1648, 1806
BsoBI CYCGRG 4 cut(s) 281, 929, 1863, 2195
Bsp1286I GDGCHC 8 cut(s) 156, 834, 996, 1259, 1372, 1628, 2112, 2178
Bsp13I TCCGGA 1 cut(s) 731
Bsp1407I TGTACA 1 cut(s) 2148
Bsp143I GATC 7 cut(s) 702, 715, 1588, 2163, 2283, 2353, 2419
Bsp1720I GCTNAGC 2 cut(s) 332, 2382
BspEI TCCGGA 1 cut(s) 731
BspFNI CGCG 3 cut(s) 218, 1214, 1924
BspHI TCATGA 1 cut(s) 2286
BspMAI CTGCAG 3 cut(s) 1738, 2083, 2443
BspMI ACCTGC 1 cut(s) 2322
BspPI GGATC 3 cut(s) 697, 2348, 2361
BspQI GCTCTTC 3 cut(s) 138, 1264, 1616
BspT107I GGYRCC 2 cut(s) 1166, 1367
BsrDI GCAATG 1 cut(s) 2016
BsrFI RCCGGY 4 cut(s) 69, 167, 420, 2362
BsrGI TGTACA 1 cut(s) 2148
BsrI ACTGG 4 cut(s) 505, 1071, 2175, 2434
BssAI RCCGGY 4 cut(s) 69, 167, 420, 2362
BssECI CCNNGG 8 cut(s) 269, 1481, 1797, 2089, 2155, 2194, 2195, 2196
BssMI GATC 7 cut(s) 702, 715, 1588, 2163, 2283, 2353, 2419
BssNI GRCGYC 1 cut(s) 1167
BssT1I CCWWGG 2 cut(s) 1797, 2155
Bst2UI CCWGG 5 cut(s) 657, 968, 1376, 1482, 1506
Bst4CI ACNGT 3 cut(s) 458, 1241, 2229
Bst6I CTCTTC 5 cut(s) 138, 416, 468, 1264, 1616
BstACI GRCGYC 1 cut(s) 1167
BstAUI TGTACA 1 cut(s) 2148
BstC8I GCNNGC 8 cut(s) 86, 208, 318, 338, 358, 1470, 2364, 2473
BstDSI CCRYGG 1 cut(s) 269
BstEII GGTNACC 1 cut(s) 2309
BstF5I GGATG 7 cut(s) 27, 579, 615, 673, 1905, 1976, 2299
BstFNI CGCG 3 cut(s) 218, 1214, 1924
BstH2I RGCGCY 3 cut(s) 323, 1170, 1491
BstHHI GCGC 5 cut(s) 180, 218, 322, 1169, 1490
BstKTI GATC 7 cut(s) 705, 718, 1591, 2166, 2286, 2356, 2422
BstMAI GTCTC 4 cut(s) 271, 378, 596, 1150
BstMBI GATC 7 cut(s) 702, 715, 1588, 2163, 2283, 2353, 2419
BstMCI CGRYCG 2 cut(s) 2055, 2375
BstNI CCWGG 5 cut(s) 657, 968, 1376, 1482, 1506
BstNSI RCATGY 1 cut(s) 1831
BstPI GGTNACC 1 cut(s) 2309
BstSCI CCNGG 9 cut(s) 655, 803, 957, 966, 1374, 1480, 1504, 2194, 2195
BstSFI CTRYAG 3 cut(s) 1734, 2079, 2439
BstSLI GKGCMC 1 cut(s) 1372
BstUI CGCG 3 cut(s) 218, 1214, 1924
BstV2I GAAGAC 1 cut(s) 1810
BstX2I RGATCY 1 cut(s) 2353
BstXI CCANNNNNNTGG 1 cut(s) 663
BstYI RGATCY 1 cut(s) 2353
BstZI CGGCCG 2 cut(s) 2052, 2372
Bsu36I CCTNAGG 2 cut(s) 1001, 1833
BtgI CCRYGG 1 cut(s) 269
BtgZI GCGATG 1 cut(s) 2343
BtrI CACGTC 1 cut(s) 200
BtsCI GGATG 7 cut(s) 27, 579, 615, 673, 1905, 1976, 2299
BtsI GCAGTG 2 cut(s) 1602, 2076
BtsIMutI CAGTG 7 cut(s) 996, 1024, 1096, 1246, 1602, 2076, 2234
BveI ACCTGC 1 cut(s) 2322
Cac8I GCNNGC 8 cut(s) 86, 208, 318, 338, 358, 1470, 2364, 2473
CaiI CAGNNNCTG 3 cut(s) 1037, 1505, 2484
CciI TCATGA 1 cut(s) 2286
CciNI GCGGCCGC 1 cut(s) 2052
CfoI GCGC 5 cut(s) 180, 218, 322, 1169, 1490
Cfr10I RCCGGY 4 cut(s) 69, 167, 420, 2362
Cfr13I GGNCC 8 cut(s) 72, 82, 286, 359, 807, 1565, 1793, 1868
Cfr9I CCCGGG 1 cut(s) 2195
CseI GACGC 1 cut(s) 1836
Csp6I GTAC 5 cut(s) 623, 1237, 1338, 2149, 2459
CspCI CAANNNNNGTGG 4 cut(s) 1000, 1035, 1072, 1107
CviAII CATG 7 cut(s) 32, 1465, 1828, 1859, 1964, 2215, 2287
CviQI GTAC 5 cut(s) 623, 1237, 1338, 2149, 2459
DinI GGCGCC 1 cut(s) 1168
DpnI GATC 7 cut(s) 704, 717, 1590, 2165, 2285, 2355, 2421
DpnII GATC 7 cut(s) 702, 715, 1588, 2163, 2283, 2353, 2419
EaeI YGGCCR 4 cut(s) 1021, 1889, 2052, 2372
EagI CGGCCG 2 cut(s) 2052, 2372
Eam1104I CTCTTC 5 cut(s) 138, 416, 468, 1264, 1616
EarI CTCTTC 5 cut(s) 138, 416, 468, 1264, 1616
EciI GGCGGA 1 cut(s) 1281
Ecl136II GAGCTC 4 cut(s) 154, 832, 2110, 2176
EclXI CGGCCG 2 cut(s) 2052, 2372
Eco130I CCWWGG 2 cut(s) 1797, 2155
Eco147I AGGCCT 1 cut(s) 966
Eco24I GRGCYC 4 cut(s) 156, 834, 2112, 2178
Eco47I GGWCC 2 cut(s) 807, 1793
Eco47III AGCGCT 1 cut(s) 321
Eco52I CGGCCG 2 cut(s) 2052, 2372
Eco53kI GAGCTC 4 cut(s) 154, 832, 2110, 2176
Eco57I CTGAAG 2 cut(s) 837, 1208
Eco81I CCTNAGG 2 cut(s) 1001, 1833
Eco88I CYCGRG 4 cut(s) 281, 929, 1863, 2195
Eco91I GGTNACC 1 cut(s) 2309
EcoICRI GAGCTC 4 cut(s) 154, 832, 2110, 2176
EcoO109I RGGNCCY 1 cut(s) 1793
EcoO65I GGTNACC 1 cut(s) 2309
EcoRII CCWGG 5 cut(s) 655, 966, 1374, 1480, 1504
EcoT14I CCWWGG 2 cut(s) 1797, 2155
EcoT38I GRGCYC 4 cut(s) 156, 834, 2112, 2178
EgeI GGCGCC 1 cut(s) 1168
EheI GGCGCC 1 cut(s) 1168
ErhI CCWWGG 2 cut(s) 1797, 2155
FaeI CATG 7 cut(s) 35, 1468, 1831, 1862, 1967, 2218, 2290
FalI AAGNNNNNCTT 6 cut(s) 8, 40, 839, 871, 1420, 1452
FaqI GGGAC 6 cut(s) 350, 422, 820, 1157, 1648, 1806
FatI CATG 7 cut(s) 31, 1464, 1827, 1858, 1963, 2214, 2286
FauI CCCGC 5 cut(s) 447, 1207, 1226, 1275, 1357
FauNDI CATATG 1 cut(s) 1630
FbaI TGATCA 2 cut(s) 715, 2163
FokI GGATG 7 cut(s) 14, 566, 602, 680, 1912, 1963, 2306
FriOI GRGCYC 4 cut(s) 156, 834, 2112, 2178
FspBI CTAG 4 cut(s) 516, 744, 851, 891
FspI TGCGCA 1 cut(s) 179
GlaI GCGC 5 cut(s) 179, 217, 321, 1168, 1489
GsuI CTGGAG 5 cut(s) 827, 1464, 2192, 2414, 2423
HaeII RGCGCY 3 cut(s) 323, 1170, 1491
HgaI GACGC 1 cut(s) 1836
HhaI GCGC 5 cut(s) 180, 218, 322, 1169, 1490
Hin1I GRCGYC 1 cut(s) 1167
Hin1II CATG 7 cut(s) 35, 1468, 1831, 1862, 1967, 2218, 2290
Hin6I GCGC 5 cut(s) 178, 216, 320, 1167, 1488
HinP1I GCGC 5 cut(s) 178, 216, 320, 1167, 1488
HindIII AAGCTT 2 cut(s) 1547, 1808
HinfI GANTC 8 cut(s) 112, 279, 508, 1056, 1219, 1276, 1750, 1762
HphI GGTGA 8 cut(s) 157, 586, 604, 1106, 1316, 1544, 2173, 2321
Hpy166II GTNNAC 5 cut(s) 241, 406, 1555, 1928, 2149
Hpy8I GTNNAC 5 cut(s) 241, 406, 1555, 1928, 2149
HpyCH4III ACNGT 3 cut(s) 458, 1241, 2229
HpyCH4IV ACGT 1 cut(s) 199
HpySE526I ACGT 1 cut(s) 199
Hsp92I GRCGYC 1 cut(s) 1167
Hsp92II CATG 7 cut(s) 35, 1468, 1831, 1862, 1967, 2218, 2290
HspAI GCGC 5 cut(s) 178, 216, 320, 1167, 1488
KasI GGCGCC 1 cut(s) 1166
KflI GGGWCCC 1 cut(s) 1793
Kpn2I TCCGGA 1 cut(s) 731
KroI GCCGGC 1 cut(s) 2362
KroNI GCCGGC 1 cut(s) 2364
Ksp22I TGATCA 2 cut(s) 715, 2163
Kzo9I GATC 7 cut(s) 702, 715, 1588, 2163, 2283, 2353, 2419
LguI GCTCTTC 3 cut(s) 138, 1264, 1616
LweI GCATC 4 cut(s) 658, 1043, 1330, 1926
MaeI CTAG 4 cut(s) 516, 744, 851, 891
MaeII ACGT 1 cut(s) 199
MaeIII GTNAC 5 cut(s) 61, 109, 452, 689, 2309
MalI GATC 7 cut(s) 704, 717, 1590, 2165, 2285, 2355, 2421
MboI GATC 7 cut(s) 702, 715, 1588, 2163, 2283, 2353, 2419
MfeI CAATTG 1 cut(s) 1740
MflI RGATCY 1 cut(s) 2353
MhlI GDGCHC 8 cut(s) 156, 834, 996, 1259, 1372, 1628, 2112, 2178
MlsI TGGCCA 2 cut(s) 1023, 1891
MluCI AATT 8 cut(s) 490, 758, 1012, 1085, 1098, 1331, 1350, 1740
MluI ACGCGT 1 cut(s) 1922
MluNI TGGCCA 2 cut(s) 1023, 1891
Mly113I GGCGCC 1 cut(s) 1167
MlyI GAGTC 2 cut(s) 106, 273
MmeI TCCRAC 4 cut(s) 652, 885, 924, 1495
Mox20I TGGCCA 2 cut(s) 1023, 1891
MroI TCCGGA 1 cut(s) 731
MroNI GCCGGC 1 cut(s) 2362
MroXI GAANNNNTTC 1 cut(s) 1013
MscI TGGCCA 2 cut(s) 1023, 1891
MseI TTAA 5 cut(s) 231, 482, 549, 2322, 2412
MslI CAYNNNNRTG 3 cut(s) 345, 1560, 2066
Msp20I TGGCCA 2 cut(s) 1023, 1891
MspA1I CMGCKG 6 cut(s) 183, 313, 1228, 1475, 1583, 2401
MspR9I CCNGG 9 cut(s) 657, 805, 959, 968, 1376, 1482, 1506, 2196, 2197
MunI CAATTG 1 cut(s) 1740
MvaI CCWGG 5 cut(s) 657, 968, 1376, 1482, 1506
MvnI CGCG 3 cut(s) 218, 1214, 1924
NaeI GCCGGC 1 cut(s) 2364
NarI GGCGCC 1 cut(s) 1167
NciI CCSGG 4 cut(s) 805, 959, 2196, 2197
NdeI CATATG 1 cut(s) 1630
NdeII GATC 7 cut(s) 702, 715, 1588, 2163, 2283, 2353, 2419
NgoMIV GCCGGC 1 cut(s) 2362
NlaIII CATG 7 cut(s) 35, 1468, 1831, 1862, 1967, 2218, 2290
NmeAIII GCCGAG 2 cut(s) 357, 2070
NmuCI GTSAC 3 cut(s) 61, 109, 2309
NotI GCGGCCGC 1 cut(s) 2052
NsbI TGCGCA 1 cut(s) 179
NspI RCATGY 1 cut(s) 1831
OliI CACNNNNGTG 1 cut(s) 2066
PaeR7I CTCGAG 2 cut(s) 929, 1863
PagI TCATGA 1 cut(s) 2286
PceI AGGCCT 1 cut(s) 966
PciSI GCTCTTC 3 cut(s) 138, 1264, 1616
PdiI GCCGGC 1 cut(s) 2364
PdmI GAANNNNTTC 1 cut(s) 1013
PfeI GAWTC 6 cut(s) 508, 1056, 1219, 1276, 1750, 1762
PflFI GACNNNGTC 2 cut(s) 115, 347
PflMI CCANNNNNTGG 2 cut(s) 2306, 2432
PfoI TCCNGGA 2 cut(s) 803, 957
PleI GAGTC 2 cut(s) 106, 273
PluTI GGCGCC 1 cut(s) 1170
PpsI GAGTC 2 cut(s) 106, 273
PpuMI RGGWCCY 1 cut(s) 1793
Psp124BI GAGCTC 4 cut(s) 156, 834, 2112, 2178
Psp5II RGGWCCY 1 cut(s) 1793
Psp6I CCWGG 5 cut(s) 655, 966, 1374, 1480, 1504
PspEI GGTNACC 1 cut(s) 2309
PspGI CCWGG 5 cut(s) 655, 966, 1374, 1480, 1504
PspPI GGNCC 8 cut(s) 72, 82, 286, 359, 807, 1565, 1793, 1868
PspPPI RGGWCCY 1 cut(s) 1793
PspXI VCTCGAGB 2 cut(s) 929, 1863
PstI CTGCAG 3 cut(s) 1738, 2083, 2443
PstNI CAGNNNCTG 3 cut(s) 1037, 1505, 2484
PsuI RGATCY 1 cut(s) 2353
PsyI GACNNNGTC 2 cut(s) 115, 347
PvuII CAGCTG 5 cut(s) 183, 313, 1228, 1583, 2401
RsaI GTAC 5 cut(s) 624, 1238, 1339, 2150, 2460
RsaNI GTAC 5 cut(s) 623, 1237, 1338, 2149, 2459
RseI CAYNNNNRTG 3 cut(s) 345, 1560, 2066
SacI GAGCTC 4 cut(s) 156, 834, 2112, 2178
SapI GCTCTTC 3 cut(s) 138, 1264, 1616
SaqAI TTAA 5 cut(s) 231, 482, 549, 2322, 2412
Sau3AI GATC 7 cut(s) 702, 715, 1588, 2163, 2283, 2353, 2419
Sau96I GGNCC 8 cut(s) 72, 82, 286, 359, 807, 1565, 1793, 1868
SchI GAGTC 2 cut(s) 106, 273
ScrFI CCNGG 9 cut(s) 657, 805, 959, 968, 1376, 1482, 1506, 2196, 2197
SduI GDGCHC 8 cut(s) 156, 834, 996, 1259, 1372, 1628, 2112, 2178
SfaNI GCATC 4 cut(s) 658, 1043, 1330, 1926
SfcI CTRYAG 3 cut(s) 1734, 2079, 2439
SfiI GGCCNNNNNGGCC 1 cut(s) 2126
SfoI GGCGCC 1 cut(s) 1168
Sfr274I CTCGAG 2 cut(s) 929, 1863
SgrAI CRCCGGYG 1 cut(s) 167
SinI GGWCC 2 cut(s) 807, 1793
SlaI CTCGAG 2 cut(s) 929, 1863
SmaI CCCGGG 1 cut(s) 2197
SmiMI CAYNNNNRTG 3 cut(s) 345, 1560, 2066
SmlI CTYRAG 2 cut(s) 929, 1863
SmoI CTYRAG 2 cut(s) 929, 1863
Sse9I AATT 8 cut(s) 490, 758, 1012, 1085, 1098, 1331, 1350, 1740
SseBI AGGCCT 1 cut(s) 966
SspDI GGCGCC 1 cut(s) 1166
SspMI CTAG 4 cut(s) 516, 744, 851, 891
SstI GAGCTC 4 cut(s) 156, 834, 2112, 2178
StuI AGGCCT 1 cut(s) 966
StyD4I CCNGG 9 cut(s) 655, 803, 957, 966, 1374, 1480, 1504, 2194, 2195
StyI CCWWGG 2 cut(s) 1797, 2155
TaaI ACNGT 3 cut(s) 458, 1241, 2229
TaiI ACGT 1 cut(s) 202
TaqI TCGA 7 cut(s) 136, 679, 930, 945, 1821, 1864, 2240
TasI AATT 8 cut(s) 490, 758, 1012, 1085, 1098, 1331, 1350, 1740
TatI WGTACW 3 cut(s) 622, 1337, 2148
TauI GCSGC 7 cut(s) 1364, 1463, 1475, 1512, 2054, 2057, 2374
TfiI GAWTC 6 cut(s) 508, 1056, 1219, 1276, 1750, 1762
Tru1I TTAA 5 cut(s) 231, 482, 549, 2322, 2412
Tru9I TTAA 5 cut(s) 231, 482, 549, 2322, 2412
TscAI CASTG 7 cut(s) 996, 1024, 1096, 1246, 1609, 2083, 2234
TseFI GTSAC 3 cut(s) 61, 109, 2309
Tsp45I GTSAC 3 cut(s) 61, 109, 2309
TspDTI ATGAA 6 cut(s) 16, 38, 1847, 1916, 2222, 2280
TspGWI ACGGA 4 cut(s) 258, 258, 1242, 1313
TspMI CCCGGG 1 cut(s) 2195
TspRI CASTG 7 cut(s) 996, 1024, 1096, 1246, 1609, 2083, 2234
Tth111I GACNNNGTC 2 cut(s) 115, 347
Van91I CCANNNNNTGG 2 cut(s) 2306, 2432
VpaK11BI GGWCC 2 cut(s) 807, 1793
XapI RAATTY 4 cut(s) 1085, 1098, 1331, 1350
XceI RCATGY 1 cut(s) 1831
XhoI CTCGAG 2 cut(s) 929, 1863
XmaI CCCGGG 1 cut(s) 2195
XmnI GAANNNNTTC 1 cut(s) 1013
XspI CTAG 4 cut(s) 516, 744, 851, 891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.