Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003416

Physical Location & Seq

Genomic Coordinates Reverse (-)

25672407 .. 25673116

Gene Structure 710 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1166300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 441 bp

ATGACTGCGGCCGCCAACACCACTGTGATCGCCACTGCAGGAACTCTCGGCTACAACGCACCTGAGCTCTCAAAGTCCAAGAAGGCCACAACAAAGACTGATGTGTACAGCCTTGGGGTGCTCATACTGGAGCTGCTGACAGGAAAATCCCCCGGGGAACCGATGAACAGCATGGATTTGCCCCAATGGGTGGCATCAATTGTGAAAGAGGAGTGGACAAATGAAGTTTTCGACTTGGAGCTCATGAGGGATGTGCCAACCATAGGTGACCTGCTGCTTAACACATTGAAGTTGGCTCTGCATTGTGTGGATCCATCGCCGGCTGCGCGGCCGGAAGCTCAGCTGGTTCTGCAGCAGCTGGAGGAGATCAAGCCTGATCAGGCAACTGGTGGTTCTGCAGCTGAAGGAGGAGCAGAAGTACCGCCACAAGAAGCTGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

147

Amino Acids

15.48

Weight (kDa)

4.42

Isoelectric Point (pI)

31.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	11 - 118	7.4e-11	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	17 - 118	1.3e-07	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003416)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51740
fragaria_vesca	FvH4_6g10640 FvH4_6g10640 FvH4_7g25350
malus_domestica	MD01G1159600.v1.1 MD04G1166300.v1.1 MD04G1166400.v1.1 MD07G1228100.v1.1
prunus_persica	Prupe.2G257600_v2.0.a1 Prupe.6G284600_v2.0.a1
pyrus_communis	pycom04g14780 pycom07g20650 pycom12g16950
rosa_chinensis	RchiOBHm_Chr1g0371261 RchiOBHm_Chr1g0371271 RchiOBHm_Chr3g0461661
rosa_laevigata	RLG00000024909 RLG00000026975
rosa_multiflora	Rmu_sc0001978.1_g000006 Rmu_sc0012406.1_g000007
rosa_roxburghii	Rroxscaffold_4G00285830 Rroxscaffold_6G00418060
rosa_rugosa	Rorug01G0362500 Rorug03G0052700
rosa_samantha	Rh1AG372600 Rh1BG336100 Rh1CG349500 Rh1DG367200 Rh3AG111000 Rh3AG111100 Rh3BG114000 Rh3CG116000 Rh3CG116100 Rh3DG115900
rosa_wichuraiana	Rw1G032770 Rw3G009390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	279
AccB7I	CCANNNNNTGG	1 cut(s)	190
AccII	CGCG	1 cut(s)	328
AciI	CCGC	4 cut(s)	8, 12, 328, 422
AclWI	GGATC	2 cut(s)	305, 318
AcoI	YGGCCR	2 cut(s)	9, 329
AcuI	CTGAAG	1 cut(s)	423
AfaI	GTAC	2 cut(s)	107, 420
AfiI	CCNNNNNNNGG	2 cut(s)	190, 263
AgsI	TTSAA	1 cut(s)	289
AleI	CACNNNNGTG	1 cut(s)	23
AluBI	AGCT	8 cut(s)	67, 133, 241, 338, 343, 358, 401, 434
AluI	AGCT	8 cut(s)	67, 133, 241, 338, 343, 358, 401, 434
Alw21I	GWGCWC	3 cut(s)	69, 123, 243
AlwI	GGATC	2 cut(s)	305, 318
AlwNI	CAGNNNCTG	1 cut(s)	358
Ama87I	CYCGRG	1 cut(s)	152
AoxI	GGCC	3 cut(s)	9, 84, 329
ApeKI	GCWGC	6 cut(s)	133, 274, 323, 352, 355, 398
AspLEI	GCGC	1 cut(s)	328
AsuC2I	CCSGG	2 cut(s)	153, 154
AsuHPI	GGTGA	1 cut(s)	278
AvaI	CYCGRG	1 cut(s)	152
BamHI	GGATCC	1 cut(s)	310
BanII	GRGCYC	2 cut(s)	69, 243
Bbv12I	GWGCWC	3 cut(s)	69, 123, 243
BbvI	GCAGC	6 cut(s)	120, 261, 310, 364, 367, 410
BccI	CCATC	1 cut(s)	322
BclI	TGATCA	1 cut(s)	376
BcnI	CCSGG	2 cut(s)	153, 154
BfmI	CTRYAG	3 cut(s)	36, 350, 396
BfuAI	ACCTGC	1 cut(s)	279
BisI	GCNGC	9 cut(s)	9, 12, 134, 275, 324, 329, 353, 356, 399
BlpI	GCTNAGC	1 cut(s)	339
BlsI	GCNGC	9 cut(s)	10, 13, 135, 276, 325, 330, 354, 357, 400
Bme1390I	CCNGG	2 cut(s)	153, 154
BmeT110I	CYCGRG	1 cut(s)	152
BmiI	GGNNCC	2 cut(s)	159, 312
BmrFI	CCNGG	2 cut(s)	153, 154
BmsI	GCATC	1 cut(s)	203
BpmI	CTGGAG	2 cut(s)	149, 380
Bpu10I	CCTNAGC	1 cut(s)	63
Bpu1102I	GCTNAGC	1 cut(s)	339
BpuMI	CCSGG	2 cut(s)	153, 154
BsaJI	CCNNGG	4 cut(s)	112, 151, 152, 153
BsaXI	ACNNNNNCTCC	2 cut(s)	402, 432
Bsc4I	CCNNNNNNNGG	2 cut(s)	190, 263
Bse118I	RCCGGY	1 cut(s)	319
Bse1I	ACTGG	2 cut(s)	132, 391
BseDI	CCNNGG	4 cut(s)	112, 151, 152, 153
BseGI	GGATG	1 cut(s)	256
BseLI	CCNNNNNNNGG	2 cut(s)	190, 263
BseMII	CTCAG	3 cut(s)	54, 353, 426
BseNI	ACTGG	2 cut(s)	132, 391
BseRI	GAGGAG	3 cut(s)	224, 377, 423
BseX3I	CGGCCG	2 cut(s)	9, 329
BseXI	GCAGC	6 cut(s)	120, 261, 310, 364, 367, 410
Bsh1236I	CGCG	1 cut(s)	328
Bsh1285I	CGRYCG	2 cut(s)	12, 332
BshFI	GGCC	3 cut(s)	11, 86, 331
BsiEI	CGRYCG	2 cut(s)	12, 332
BsiHKAI	GWGCWC	3 cut(s)	69, 123, 243
BsiHKCI	CYCGRG	1 cut(s)	152
BsiSI	CCGG	3 cut(s)	153, 320, 332
BslI	CCNNNNNNNGG	2 cut(s)	190, 263
BsnI	GGCC	3 cut(s)	11, 86, 331
BsoBI	CYCGRG	1 cut(s)	152
Bsp1286I	GDGCHC	3 cut(s)	69, 123, 243
Bsp1407I	TGTACA	1 cut(s)	105
Bsp143I	GATC	4 cut(s)	27, 310, 366, 376
Bsp1720I	GCTNAGC	1 cut(s)	339
BspACI	CCGC	4 cut(s)	8, 12, 328, 422
BspANI	GGCC	3 cut(s)	11, 86, 331
BspCNI	CTCAG	3 cut(s)	55, 352, 427
BspFNI	CGCG	1 cut(s)	328
BspHI	TCATGA	1 cut(s)	243
BspLI	GGNNCC	2 cut(s)	159, 312
BspMAI	CTGCAG	3 cut(s)	40, 354, 400
BspMI	ACCTGC	1 cut(s)	279
BspPI	GGATC	2 cut(s)	305, 318
BsrFI	RCCGGY	1 cut(s)	319
BsrGI	TGTACA	1 cut(s)	105
BsrI	ACTGG	2 cut(s)	132, 391
BssAI	RCCGGY	1 cut(s)	319
BssECI	CCNNGG	4 cut(s)	112, 151, 152, 153
BssMI	GATC	4 cut(s)	27, 310, 366, 376
BssT1I	CCWWGG	1 cut(s)	112
Bst4CI	ACNGT	1 cut(s)	25
BstAUI	TGTACA	1 cut(s)	105
BstC8I	GCNNGC	1 cut(s)	321
BstDEI	CTNAG	3 cut(s)	63, 339, 435
BstEII	GGTNACC	1 cut(s)	266
BstF5I	GGATG	1 cut(s)	256
BstFNI	CGCG	1 cut(s)	328
BstHHI	GCGC	1 cut(s)	328
BstKTI	GATC	4 cut(s)	30, 313, 369, 379
BstMBI	GATC	4 cut(s)	27, 310, 366, 376
BstMCI	CGRYCG	2 cut(s)	12, 332
BstMWI	GCNNNNNNNGC	2 cut(s)	325, 349
BstPI	GGTNACC	1 cut(s)	266
BstSCI	CCNGG	2 cut(s)	151, 152
BstSFI	CTRYAG	3 cut(s)	36, 350, 396
BstUI	CGCG	1 cut(s)	328
BstV1I	GCAGC	6 cut(s)	120, 261, 310, 364, 367, 410
BstX2I	RGATCY	1 cut(s)	310
BstYI	RGATCY	1 cut(s)	310
BstZI	CGGCCG	2 cut(s)	9, 329
BsuRI	GGCC	3 cut(s)	11, 86, 331
BtgZI	GCGATG	1 cut(s)	300
BtsCI	GGATG	1 cut(s)	256
BtsI	GCAGTG	1 cut(s)	33
BtsIMutI	CAGTG	2 cut(s)	21, 33
BveI	ACCTGC	1 cut(s)	279
Cac8I	GCNNGC	1 cut(s)	321
CaiI	CAGNNNCTG	1 cut(s)	358
CciI	TCATGA	1 cut(s)	243
CciNI	GCGGCCGC	1 cut(s)	9
CfoI	GCGC	1 cut(s)	328
Cfr10I	RCCGGY	1 cut(s)	319
Cfr9I	CCCGGG	1 cut(s)	152
Csp6I	GTAC	2 cut(s)	106, 419
CviAII	CATG	2 cut(s)	172, 244
CviQI	GTAC	2 cut(s)	106, 419
DdeI	CTNAG	3 cut(s)	63, 339, 435
DpnI	GATC	4 cut(s)	29, 312, 368, 378
DpnII	GATC	4 cut(s)	27, 310, 366, 376
EaeI	YGGCCR	2 cut(s)	9, 329
EagI	CGGCCG	2 cut(s)	9, 329
Ecl136II	GAGCTC	2 cut(s)	67, 241
EclXI	CGGCCG	2 cut(s)	9, 329
Eco130I	CCWWGG	1 cut(s)	112
Eco24I	GRGCYC	2 cut(s)	69, 243
Eco52I	CGGCCG	2 cut(s)	9, 329
Eco53kI	GAGCTC	2 cut(s)	67, 241
Eco57I	CTGAAG	1 cut(s)	423
Eco88I	CYCGRG	1 cut(s)	152
Eco91I	GGTNACC	1 cut(s)	266
EcoICRI	GAGCTC	2 cut(s)	67, 241
EcoO65I	GGTNACC	1 cut(s)	266
EcoT14I	CCWWGG	1 cut(s)	112
EcoT38I	GRGCYC	2 cut(s)	69, 243
ErhI	CCWWGG	1 cut(s)	112
FaeI	CATG	2 cut(s)	175, 247
FaiI	YATR	4 cut(s)	125, 173, 245, 263
FatI	CATG	2 cut(s)	171, 243
FbaI	TGATCA	1 cut(s)	376
Fnu4HI	GCNGC	9 cut(s)	9, 12, 134, 275, 324, 329, 353, 356, 399
FokI	GGATG	1 cut(s)	263
FriOI	GRGCYC	2 cut(s)	69, 243
Fsp4HI	GCNGC	9 cut(s)	9, 12, 134, 275, 324, 329, 353, 356, 399
GlaI	GCGC	1 cut(s)	327
GluI	GCNGC	9 cut(s)	9, 12, 134, 275, 324, 329, 353, 356, 399
GsuI	CTGGAG	2 cut(s)	149, 380
HaeIII	GGCC	3 cut(s)	11, 86, 331
HapII	CCGG	3 cut(s)	153, 320, 332
HhaI	GCGC	1 cut(s)	328
Hin1II	CATG	2 cut(s)	175, 247
Hin6I	GCGC	1 cut(s)	326
HinP1I	GCGC	1 cut(s)	326
HpaII	CCGG	3 cut(s)	153, 320, 332
HphI	GGTGA	1 cut(s)	278
Hpy166II	GTNNAC	2 cut(s)	106, 216
Hpy188III	TCNNGA	1 cut(s)	244
Hpy8I	GTNNAC	2 cut(s)	106, 216
HpyAV	CCTTC	2 cut(s)	76, 398
HpyCH4III	ACNGT	1 cut(s)	25
HpyCH4V	TGCA	4 cut(s)	38, 301, 352, 398
HpyF10VI	GCNNNNNNNGC	2 cut(s)	325, 349
HpyF3I	CTNAG	3 cut(s)	63, 339, 435
Hsp92II	CATG	2 cut(s)	175, 247
HspAI	GCGC	1 cut(s)	326
KroI	GCCGGC	1 cut(s)	319
KroNI	GCCGGC	1 cut(s)	321
Ksp22I	TGATCA	1 cut(s)	376
Kzo9I	GATC	4 cut(s)	27, 310, 366, 376
LmnI	GCTCC	3 cut(s)	130, 238, 410
Lsp1109I	GCAGC	6 cut(s)	120, 261, 310, 364, 367, 410
LweI	GCATC	1 cut(s)	203
MaeIII	GTNAC	1 cut(s)	266
MalI	GATC	4 cut(s)	29, 312, 368, 378
MboI	GATC	4 cut(s)	27, 310, 366, 376
MfeI	CAATTG	1 cut(s)	198
MflI	RGATCY	1 cut(s)	310
MhlI	GDGCHC	3 cut(s)	69, 123, 243
MluCI	AATT	1 cut(s)	198
MnlI	CCTC	4 cut(s)	202, 240, 355, 401
MroNI	GCCGGC	1 cut(s)	319
MseI	TTAA	1 cut(s)	279
MslI	CAYNNNNRTG	1 cut(s)	23
MspA1I	CMGCKG	3 cut(s)	343, 358, 401
MspI	CCGG	3 cut(s)	153, 320, 332
MspR9I	CCNGG	2 cut(s)	153, 154
MunI	CAATTG	1 cut(s)	198
MvnI	CGCG	1 cut(s)	328
MwoI	GCNNNNNNNGC	2 cut(s)	325, 349
NaeI	GCCGGC	1 cut(s)	321
NciI	CCSGG	2 cut(s)	153, 154
NdeII	GATC	4 cut(s)	27, 310, 366, 376
NgoMIV	GCCGGC	1 cut(s)	319
NlaIII	CATG	2 cut(s)	175, 247
NlaIV	GGNNCC	2 cut(s)	159, 312
NmeAIII	GCCGAG	1 cut(s)	27
NmuCI	GTSAC	1 cut(s)	266
NotI	GCGGCCGC	1 cut(s)	9
OliI	CACNNNNGTG	1 cut(s)	23
PagI	TCATGA	1 cut(s)	243
PdiI	GCCGGC	1 cut(s)	321
PflMI	CCANNNNNTGG	1 cut(s)	190
PkrI	GCNGC	9 cut(s)	10, 13, 135, 276, 325, 330, 354, 357, 400
Psp124BI	GAGCTC	2 cut(s)	69, 243
PspEI	GGTNACC	1 cut(s)	266
PspN4I	GGNNCC	2 cut(s)	159, 312
PstI	CTGCAG	3 cut(s)	40, 354, 400
PstNI	CAGNNNCTG	1 cut(s)	358
PsuI	RGATCY	1 cut(s)	310
PvuII	CAGCTG	3 cut(s)	343, 358, 401
RsaI	GTAC	2 cut(s)	107, 420
RsaNI	GTAC	2 cut(s)	106, 419
RseI	CAYNNNNRTG	1 cut(s)	23
SacI	GAGCTC	2 cut(s)	69, 243
SaqAI	TTAA	1 cut(s)	279
SatI	GCNGC	9 cut(s)	9, 12, 134, 275, 324, 329, 353, 356, 399
Sau3AI	GATC	4 cut(s)	27, 310, 366, 376
ScrFI	CCNGG	2 cut(s)	153, 154
SduI	GDGCHC	3 cut(s)	69, 123, 243
SfaNI	GCATC	1 cut(s)	203
SfcI	CTRYAG	3 cut(s)	36, 350, 396
SmaI	CCCGGG	1 cut(s)	154
SmiMI	CAYNNNNRTG	1 cut(s)	23
Sse9I	AATT	1 cut(s)	198
SsiI	CCGC	4 cut(s)	8, 12, 328, 422
SstI	GAGCTC	2 cut(s)	69, 243
StyD4I	CCNGG	2 cut(s)	151, 152
StyI	CCWWGG	1 cut(s)	112
TaaI	ACNGT	1 cut(s)	25
TaqI	TCGA	1 cut(s)	231
TasI	AATT	1 cut(s)	198
TatI	WGTACW	1 cut(s)	105
TauI	GCSGC	3 cut(s)	11, 14, 331
Tru1I	TTAA	1 cut(s)	279
Tru9I	TTAA	1 cut(s)	279
TscAI	CASTG	2 cut(s)	28, 40
TseFI	GTSAC	1 cut(s)	266
TseI	GCWGC	6 cut(s)	133, 274, 323, 352, 355, 398
Tsp45I	GTSAC	1 cut(s)	266
TspDTI	ATGAA	2 cut(s)	179, 237
TspMI	CCCGGG	1 cut(s)	152
TspRI	CASTG	2 cut(s)	28, 40
Van91I	CCANNNNNTGG	1 cut(s)	190
XmaI	CCCGGG	1 cut(s)	152

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.