MD01G1159600.v1.1

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003416
Physical Location & Seq
Reverse (-)
26582481 .. 26585115
2635 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1159600.v1.1.491

Sequence Viewer

Length: 2469 bp
ATGGACAGCAAAGATCAAATCTTTCACACATTTTCCGACTACCCATCTCAAAATTGGCGCCGGATTTCCCACAAGAAGAAGGAAAAATGGAAGTTTCTTCCTCTGCAATCCAAATGCGCGCACTTCTTCCTCTTTCTCCACCTCCTCCTCTCCTTCTTCCACCCGGCCCGCCCCGAAATATGGGACGGCGTCATTGTCACGGCGGCGGACTACCAGTCCCTTCAAGCAATCAAGCACGAGCTCGACGACCCAAAGGGGTTCTTAAGGAGCTGGAACGACAGCGGATACGGAGCCTGCTCCGGCGGCTGGGCCGGAATCAAGTGCGCTCAGGGGCAGGTGATTGTGATCCAGCTTCCATGGAAGGGGTTAGGTGGCAGAATCTCCGAAAAAATAGGACAGCTGCAGGCTCTGAGGAAGCTCAGCCTCCATGACAACCAGATTGGAGGGTCAATCCCTCAATCTTTAGGGTACCTTCCAAGCCTCAGGGGAGTTCAATTATTTAGCAATAAACTTTCTGGTTCAATCCCAATTTCTTTAGGGTTCAGTCCTCTGCTTCAAAACCTCGATCTCAGCAACAATTTGCTAGTTGATAAAATCCCTGACAGCATTTTTAACTCCACCAAACTTAACAGCCTCAATCTGAGCTTCAATTCGCTGTCGGGGTCCATCCCAGGTAGTCTCTCTCGCTCACGTTCCCTCGTATTCCTTTCTCTCCACCACAACAATCTTTCCGGCTCGATCCCAGAGTCTTTAGGCAGGTTGAGTGAGCTTCAAGAGCTTGAACTTGGAAATAACCAGTTCAATGGAGCTATATCGAATGAAATAGGGCGGCTTTCGAGGGTTAGAATTTTAGATTTTTCAAACAATGCCATAAATGGAAGCTTGCCTTCTAGCTTCTCCAATCTCTCCTCGCTAATCCAGCTGAATCTAGAAGGAAACAACATCGAAAGCGAAATCCCGGAAGGTCTTGGAAGCTTCAAAAACCTCTCTGTTCTTAACTTGAGGAGGAACAAATTGCAGGGTTCGATTCCTGCAGCTCTTGGCAACATTTCGACGCTCACCCGCCTCGATTTGTCGCTGAATAATGTCAGTGGTGAAATTCCAGCTTCTCTTGCCGGTCTATCACATCTTGGTTTCCTCAATGTTTCATACAACAACCTCTCTGGTGCTGTCCCGGCTCTTCTCTCTCAGAAGTTCAATTCGAGCTCTTTCGCCGGGAATGTTCAGCTGTGCGGGTACAGTGCTTCAACTCCATGCCCTTCCGAGGCACCATCTCAGAGTGTCCCGCCTCCGGGCCCAGAGACTTCGAAGCACCGCCGCAGACTTAGTACCAAAGATAAAATTCTTATAGCAGCAGGGGTTCTTTTGTTAGTGTTGTTTTTACTCTGTTGCATTCTGCTGTGCTGTTTGATAAGAGGAAGAGCTGCATCGAAAGGCACGGATGGCGCAGGCGTGGCGAGGGCTGGAGCTGCGAGGACTGAAAAGGGTGTTCCGGCAGTTGGGGGAGAAGCTGAATCCGGCGGAGAGGCTGGAGGAAAACTGGTACATTTTGACGGGCCAATGGCTTTCGCAGCAGATGATCTTTTGTGTGCAACCGCGGAGATTATGGGGAAAAGCACTTTTGGGACTGTCTACAAGGCGACATTGGAGGATGGGAGTGAAGTTGCAGTGAAGAGATTGAGAGAAAAGATTACGAAAAGCCAGAGGGAATTCGAGACGGAAGTGAACGTGCTTGGGAAAATCAGGCACCCGAATCTTCTGGCTCTGAGGGCTTATTACTTGGGACCTAAGGGAGAAAAGCTTCTGGTTTTCGATTACATGCCGAAAGGGAGTCTTGCAGCATTCCTCCATGCTCGAGGGCCGGAAACTCCGATTGATTGGCCAACACGAATGGGAATAGCAAAGGGCATGGCAAGAGGTCTCTCCTACCTCCACGGCAATGAGAACATTATCCATGGAAACCTCACATCCAGCAATATTCTACTTGATGAGCAAAACAGCGCCAAGATCTCGGACTACGGCCTCTCCCGCCTCATGACAGCCGCTGCCAATTCAAATGTGATTGCAACCGCCAGGGCACTAGGGTACCGAGCCCCGGAGCTCTCAAAGCTCAAGAAGGCCAACACGAAAACTGATGTTTACAGCCTTGGGGTGATCATATTGGAGCTCCTTACAGGGAAATCTCCTGGTGAGCCGATGAACGGTTTGGATTTGCCGCAGTGGGTTGCCTCCATTGTGAAAGAGGAGTGGACTAACGAAGTTTTCGACTTGGAACTGATGAGGGACGCTTCTATAATCGGTGACGAGCTGCTAAACACACTGAAACTAGCTCTGCATTGCGTTGATCCTTCGCCGTCTGCAAGGCCGGAAGTTCATCAGGTTCTGCAGCAGCTAGAGGAGATTAGACCTGAAACAGCCGCCAGTTCCGGCGACGACGGAGCTGGAGCCCCTTCAGCAAGTGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

823

Amino Acids

88.52

Weight (kDa)

8.16

Isoelectric Point (pI)

39.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 69 - 108 1.6e-08 Leucine rich repeat N-terminal domain
LRR_8 PF13855 184 - 243 2.1e-07 Leucine rich repeat
LRR_8 PF13855 256 - 315 2.9e-06 Leucine rich repeat
LRR_14 PF23598 271 - 371 5e-09 Leucine-rich repeat region
LRR_8 PF13855 328 - 387 7.2e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 533 - 798 2.1e-43 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 533 - 797 5e-39 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 325
Acc36I ACCTGC 2 cut(s) 325, 747
Acc65I GGTACC 2 cut(s) 468, 2085
AccB1I GGYRCC 5 cut(s) 57, 468, 1267, 1746, 2085
AccI GTMKAC 1 cut(s) 1632
AccII CGCG 2 cut(s) 119, 1598
AclWI GGATC 3 cut(s) 340, 733, 2339
AcoI YGGCCR 1 cut(s) 1880
AcsI RAATTY 4 cut(s) 846, 1098, 1341, 1709
AcuI CTGAAG 1 cut(s) 2436
AcyI GRCGYC 2 cut(s) 58, 189
AfaI GTAC 5 cut(s) 470, 1238, 1330, 1545, 2087
AfiI CCNNNNNNNGG 9 cut(s) 180, 306, 362, 1264, 1291, 1292, 1499, 2095, 2201
AflII CTTAAG 1 cut(s) 262
AhdI GACNNNNNGTC 1 cut(s) 214
AjnI CCWGG 3 cut(s) 670, 2072, 2185
Alw21I GWGCWC 4 cut(s) 243, 1208, 2103, 2169
Alw26I GTCTC 4 cut(s) 683, 1295, 1709, 1925
AlwI GGATC 3 cut(s) 340, 733, 2339
AlwNI CAGNNNCTG 3 cut(s) 409, 2045, 2383
Ama87I CYCGRG 1 cut(s) 1854
AoxI GGCC 9 cut(s) 165, 309, 1294, 1556, 1859, 1880, 2020, 2118, 2363
ApaI GGGCCC 1 cut(s) 1298
ApoI RAATTY 4 cut(s) 846, 1098, 1341, 1709
Asp700I GAANNNNTTC 1 cut(s) 1800
Asp718I GGTACC 2 cut(s) 468, 2085
AspLEI GCGC 6 cut(s) 60, 119, 121, 326, 1448, 2003
AspS9I GGNCC 8 cut(s) 166, 309, 663, 1294, 1295, 1556, 1784, 1859
AsuC2I CCSGG 6 cut(s) 164, 959, 1175, 1216, 1293, 2096
AsuHPI GGTGA 6 cut(s) 349, 1051, 1106, 2164, 2201, 2312
AsuII TTCGAA 1 cut(s) 1307
AvaI CYCGRG 1 cut(s) 1854
AvaII GGWCC 2 cut(s) 663, 1784
AxyI CCTNAGG 2 cut(s) 482, 1788
BaeGI GKGCMC 2 cut(s) 1298, 2080
BaeI ACNNNNGTAYC 2 cut(s) 2069, 2102
BalI TGGCCA 1 cut(s) 1882
BanI GGYRCC 5 cut(s) 57, 468, 1267, 1746, 2085
BanII GRGCYC 7 cut(s) 243, 1208, 1298, 2095, 2103, 2169, 2449
BauI CACGAG 1 cut(s) 236
Bbv12I GWGCWC 4 cut(s) 243, 1208, 2103, 2169
BccI CCATC 5 cut(s) 52, 674, 1279, 1436, 1646
BceAI ACGGC 5 cut(s) 202, 216, 1951, 2035, 2338
BciT130I CCWGG 3 cut(s) 672, 2074, 2187
BciVI GTATCC 1 cut(s) 278
BclI TGATCA 1 cut(s) 2154
BcnI CCSGG 6 cut(s) 164, 959, 1175, 1216, 1293, 2096
BcoDI GTCTC 4 cut(s) 683, 1295, 1709, 1925
BfaI CTAG 6 cut(s) 584, 891, 929, 2081, 2327, 2393
BfmI CTRYAG 3 cut(s) 401, 1032, 2384
BfoI RGCGCY 2 cut(s) 61, 2004
BfrI CTTAAG 1 cut(s) 262
BfuAI ACCTGC 2 cut(s) 325, 747
BfuI GTATCC 1 cut(s) 278
BglII AGATCT 1 cut(s) 2007
BlpI GCTNAGC 1 cut(s) 419
Bme1390I CCNGG 9 cut(s) 164, 672, 959, 1175, 1216, 1293, 2074, 2096, 2187
Bme18I GGWCC 2 cut(s) 663, 1784
BmeRI GACNNNNNGTC 1 cut(s) 214
BmeT110I CYCGRG 1 cut(s) 1854
BmgT120I GGNCC 8 cut(s) 166, 309, 663, 1294, 1295, 1556, 1784, 1859
BmrFI CCNGG 9 cut(s) 164, 672, 959, 1175, 1216, 1293, 2074, 2096, 2187
BmsI GCATC 1 cut(s) 1436
BpmI CTGGAG 3 cut(s) 1485, 1551, 2463
Bpu10I CCTNAGC 1 cut(s) 327
Bpu1102I GCTNAGC 1 cut(s) 419
Bpu14I TTCGAA 1 cut(s) 1307
BpuEI CTTGAG 2 cut(s) 1021, 2096
BpuMI CCSGG 6 cut(s) 164, 959, 1175, 1216, 1293, 2096
BsaBI GATNNNNATC 1 cut(s) 344
BsaHI GRCGYC 2 cut(s) 58, 189
BsaI GGTCTC 1 cut(s) 1925
BsaJI CCNNGG 9 cut(s) 356, 670, 1263, 1596, 1933, 1954, 2073, 2094, 2146
BsaXI ACNNNNNCTCC 2 cut(s) 2009, 2039
Bsc4I CCNNNNNNNGG 9 cut(s) 180, 306, 362, 1264, 1291, 1292, 1499, 2095, 2201
Bse118I RCCGGY 1 cut(s) 1115
Bse1I ACTGG 4 cut(s) 214, 796, 1545, 2421
Bse21I CCTNAGG 2 cut(s) 482, 1788
Bse3DI GCAATG 2 cut(s) 1945, 2335
Bse8I GATNNNNATC 1 cut(s) 344
BseBI CCWGG 3 cut(s) 672, 2074, 2187
BseDI CCNNGG 9 cut(s) 356, 670, 1263, 1596, 1933, 1954, 2073, 2094, 2146
BseGI GGATG 4 cut(s) 666, 1447, 1657, 1967
BseJI GATNNNNATC 1 cut(s) 344
BseLI CCNNNNNNNGG 9 cut(s) 180, 306, 362, 1264, 1291, 1292, 1499, 2095, 2201
BseMI GCAATG 2 cut(s) 1945, 2335
BseMII CTCAG 9 cut(s) 341, 401, 433, 496, 583, 632, 1202, 1289, 1757
BseNI ACTGG 4 cut(s) 214, 796, 1545, 2421
BsePI GCGCGC 1 cut(s) 117
BseRI GAGGAG 6 cut(s) 134, 137, 898, 1018, 2258, 2411
BseSI GKGCMC 2 cut(s) 1298, 2080
BseYI CCCAGC 1 cut(s) 306
Bsh1236I CGCG 2 cut(s) 119, 1598
BshFI GGCC 9 cut(s) 167, 311, 1296, 1558, 1861, 1882, 2022, 2120, 2365
BshNI GGYRCC 5 cut(s) 57, 468, 1267, 1746, 2085
BsiHKAI GWGCWC 4 cut(s) 243, 1208, 2103, 2169
BsiHKCI CYCGRG 1 cut(s) 1854
BslFI GGGAC 7 cut(s) 197, 202, 1157, 1268, 1639, 1797, 2297
BslI CCNNNNNNNGG 9 cut(s) 180, 306, 362, 1264, 1291, 1292, 1499, 2095, 2201
BsmAI GTCTC 4 cut(s) 683, 1295, 1709, 1925
BsmBI CGTCTC 1 cut(s) 1709
BsmFI GGGAC 7 cut(s) 197, 202, 1157, 1268, 1639, 1797, 2297
BsmI GAATGC 2 cut(s) 1392, 1841
BsnI GGCC 9 cut(s) 167, 311, 1296, 1558, 1861, 1882, 2022, 2120, 2365
Bso31I GGTCTC 1 cut(s) 1925
BsoBI CYCGRG 1 cut(s) 1854
Bsp119I TTCGAA 1 cut(s) 1307
Bsp120I GGGCCC 1 cut(s) 1294
Bsp1286I GDGCHC 8 cut(s) 243, 1208, 1298, 2080, 2095, 2103, 2169, 2449
Bsp143I GATC 8 cut(s) 13, 345, 565, 738, 1579, 2007, 2154, 2344
Bsp1720I GCTNAGC 1 cut(s) 419
Bsp19I CCATGG 2 cut(s) 356, 1954
BspANI GGCC 9 cut(s) 167, 311, 1296, 1558, 1861, 1882, 2022, 2120, 2365
BspCNI CTCAG 9 cut(s) 340, 402, 432, 495, 582, 633, 1201, 1288, 1758
BspFNI CGCG 2 cut(s) 119, 1598
BspHI TCATGA 1 cut(s) 2034
BspMAI CTGCAG 3 cut(s) 405, 1036, 2388
BspMI ACCTGC 2 cut(s) 325, 747
BspPI GGATC 3 cut(s) 340, 733, 2339
BspQI GCTCTTC 2 cut(s) 1185, 1414
BspT104I TTCGAA 1 cut(s) 1307
BspT107I GGYRCC 5 cut(s) 57, 468, 1267, 1746, 2085
BspTI CTTAAG 1 cut(s) 262
BspTNI GGTCTC 1 cut(s) 1925
BsrDI GCAATG 2 cut(s) 1945, 2335
BsrFI RCCGGY 1 cut(s) 1115
BsrI ACTGG 4 cut(s) 214, 796, 1545, 2421
BssAI RCCGGY 1 cut(s) 1115
BssECI CCNNGG 9 cut(s) 356, 670, 1263, 1596, 1933, 1954, 2073, 2094, 2146
BssHII GCGCGC 1 cut(s) 117
BssMI GATC 8 cut(s) 13, 345, 565, 738, 1579, 2007, 2154, 2344
BssNI GRCGYC 2 cut(s) 58, 189
BssSI CACGAG 1 cut(s) 236
BssT1I CCWWGG 3 cut(s) 356, 1954, 2146
Bst2BI CACGAG 1 cut(s) 236
Bst2UI CCWGG 3 cut(s) 672, 2074, 2187
Bst4CI ACNGT 3 cut(s) 1241, 1630, 2204
Bst6I CTCTTC 3 cut(s) 1185, 1414, 1667
BstACI GRCGYC 2 cut(s) 58, 189
BstAFI CTTAAG 1 cut(s) 262
BstBI TTCGAA 1 cut(s) 1307
BstC8I GCNNGC 6 cut(s) 119, 169, 295, 405, 884, 1450
BstDSI CCRYGG 4 cut(s) 356, 1596, 1933, 1954
BstF5I GGATG 4 cut(s) 666, 1447, 1657, 1967
BstFNI CGCG 2 cut(s) 119, 1598
BstH2I RGCGCY 2 cut(s) 61, 2004
BstHHI GCGC 6 cut(s) 60, 119, 121, 326, 1448, 2003
BstKTI GATC 8 cut(s) 16, 348, 568, 741, 1582, 2010, 2157, 2347
BstMAI GTCTC 4 cut(s) 683, 1295, 1709, 1925
BstMBI GATC 8 cut(s) 13, 345, 565, 738, 1579, 2007, 2154, 2344
BstNI CCWGG 3 cut(s) 672, 2074, 2187
BstNSI RCATGY 1 cut(s) 1822
BstSCI CCNGG 9 cut(s) 162, 670, 957, 1173, 1214, 1291, 2072, 2094, 2185
BstSFI CTRYAG 3 cut(s) 401, 1032, 2384
BstSLI GKGCMC 2 cut(s) 1298, 2080
BstUI CGCG 2 cut(s) 119, 1598
BstX2I RGATCY 1 cut(s) 2007
BstXI CCANNNNNNTGG 1 cut(s) 803
BstYI RGATCY 1 cut(s) 2007
Bsu36I CCTNAGG 2 cut(s) 482, 1788
BsuI GTATCC 1 cut(s) 278
BsuRI GGCC 9 cut(s) 167, 311, 1296, 1558, 1861, 1882, 2022, 2120, 2365
BtgI CCRYGG 4 cut(s) 356, 1596, 1933, 1954
BtsCI GGATG 4 cut(s) 666, 1447, 1657, 1967
BtsI GCAGTG 2 cut(s) 1674, 2225
BtsIMutI CAGTG 5 cut(s) 1096, 1246, 1674, 2225, 2318
BveI ACCTGC 2 cut(s) 325, 747
Cac8I GCNNGC 6 cut(s) 119, 169, 295, 405, 884, 1450
CaiI CAGNNNCTG 3 cut(s) 409, 2045, 2383
CciI TCATGA 1 cut(s) 2034
CfoI GCGC 6 cut(s) 60, 119, 121, 326, 1448, 2003
Cfr10I RCCGGY 1 cut(s) 1115
Cfr13I GGNCC 8 cut(s) 166, 309, 663, 1294, 1295, 1556, 1784, 1859
Cfr42I CCGCGG 1 cut(s) 1599
CseI GACGC 3 cut(s) 178, 1063, 2294
Csp6I GTAC 5 cut(s) 469, 1237, 1329, 1544, 2086
CviAII CATG 8 cut(s) 357, 428, 1254, 1819, 1850, 1909, 1955, 2035
CviQI GTAC 5 cut(s) 469, 1237, 1329, 1544, 2086
DinI GGCGCC 1 cut(s) 59
DpnI GATC 8 cut(s) 15, 347, 567, 740, 1581, 2009, 2156, 2346
DpnII GATC 8 cut(s) 13, 345, 565, 738, 1579, 2007, 2154, 2344
DriI GACNNNNNGTC 1 cut(s) 214
EaeI YGGCCR 1 cut(s) 1880
Eam1104I CTCTTC 3 cut(s) 1185, 1414, 1667
Eam1105I GACNNNNNGTC 1 cut(s) 214
EarI CTCTTC 3 cut(s) 1185, 1414, 1667
EciI GGCGGA 2 cut(s) 221, 1536
Ecl136II GAGCTC 4 cut(s) 241, 1206, 2101, 2167
Eco130I CCWWGG 3 cut(s) 356, 1954, 2146
Eco24I GRGCYC 7 cut(s) 243, 1208, 1298, 2095, 2103, 2169, 2449
Eco31I GGTCTC 1 cut(s) 1925
Eco47I GGWCC 2 cut(s) 663, 1784
Eco53kI GAGCTC 4 cut(s) 241, 1206, 2101, 2167
Eco57I CTGAAG 1 cut(s) 2436
Eco81I CCTNAGG 2 cut(s) 482, 1788
Eco88I CYCGRG 1 cut(s) 1854
EcoICRI GAGCTC 4 cut(s) 241, 1206, 2101, 2167
EcoO109I RGGNCCY 1 cut(s) 1784
EcoRI GAATTC 1 cut(s) 1709
EcoRII CCWGG 3 cut(s) 670, 2072, 2185
EcoT14I CCWWGG 3 cut(s) 356, 1954, 2146
EcoT38I GRGCYC 7 cut(s) 243, 1208, 1298, 2095, 2103, 2169, 2449
EgeI GGCGCC 1 cut(s) 59
EheI GGCGCC 1 cut(s) 59
ErhI CCWWGG 3 cut(s) 356, 1954, 2146
Esp3I CGTCTC 1 cut(s) 1709
FaeI CATG 8 cut(s) 360, 431, 1257, 1822, 1853, 1912, 1958, 2038
FalI AAGNNNNNCTT 6 cut(s) 245, 277, 871, 903, 1818, 1850
FaqI GGGAC 7 cut(s) 197, 202, 1157, 1268, 1639, 1797, 2297
FatI CATG 8 cut(s) 356, 427, 1253, 1818, 1849, 1908, 1954, 2034
FauI CCCGC 5 cut(s) 176, 1070, 1226, 1293, 2036
FbaI TGATCA 1 cut(s) 2154
FblI GTMKAC 1 cut(s) 1632
FokI GGATG 4 cut(s) 653, 1454, 1664, 1954
FriOI GRGCYC 7 cut(s) 243, 1208, 1298, 2095, 2103, 2169, 2449
FspBI CTAG 6 cut(s) 584, 891, 929, 2081, 2327, 2393
GlaI GCGC 6 cut(s) 59, 118, 120, 325, 1447, 2002
GsaI CCCAGC 1 cut(s) 310
GsuI CTGGAG 3 cut(s) 1485, 1551, 2463
HaeII RGCGCY 2 cut(s) 61, 2004
HaeIII GGCC 9 cut(s) 167, 311, 1296, 1558, 1861, 1882, 2022, 2120, 2365
HgaI GACGC 3 cut(s) 178, 1063, 2294
HhaI GCGC 6 cut(s) 60, 119, 121, 326, 1448, 2003
Hin1I GRCGYC 2 cut(s) 58, 189
Hin1II CATG 8 cut(s) 360, 431, 1257, 1822, 1853, 1912, 1958, 2038
Hin6I GCGC 6 cut(s) 58, 117, 119, 324, 1446, 2001
HinP1I GCGC 6 cut(s) 58, 117, 119, 324, 1446, 2001
HindIII AAGCTT 3 cut(s) 880, 973, 1799
HinfI GANTC 8 cut(s) 315, 378, 746, 925, 1027, 1514, 1753, 1831
HphI GGTGA 6 cut(s) 349, 1051, 1106, 2164, 2201, 2312
Hpy166II GTNNAC 4 cut(s) 1633, 1726, 2140, 2250
Hpy188III TCNNGA 5 cut(s) 773, 929, 1714, 2035, 2113
Hpy8I GTNNAC 4 cut(s) 1633, 1726, 2140, 2250
Hpy99I CGWCG 4 cut(s) 248, 1057, 2435, 2438
HpyCH4III ACNGT 3 cut(s) 1241, 1630, 2204
HpyCH4IV ACGT 2 cut(s) 691, 1728
HpySE526I ACGT 2 cut(s) 691, 1728
Hsp92I GRCGYC 2 cut(s) 58, 189
Hsp92II CATG 8 cut(s) 360, 431, 1257, 1822, 1853, 1912, 1958, 2038
HspAI GCGC 6 cut(s) 58, 117, 119, 324, 1446, 2001
KasI GGCGCC 1 cut(s) 57
KpnI GGTACC 2 cut(s) 472, 2089
Ksp22I TGATCA 1 cut(s) 2154
KspI CCGCGG 1 cut(s) 1599
Kzo9I GATC 8 cut(s) 13, 345, 565, 738, 1579, 2007, 2154, 2344
LguI GCTCTTC 2 cut(s) 1185, 1414
LweI GCATC 1 cut(s) 1436
MaeI CTAG 6 cut(s) 584, 891, 929, 2081, 2327, 2393
MaeII ACGT 2 cut(s) 691, 1728
MaeIII GTNAC 2 cut(s) 196, 2300
MalI GATC 8 cut(s) 15, 347, 567, 740, 1581, 2009, 2156, 2346
MboI GATC 8 cut(s) 13, 345, 565, 738, 1579, 2007, 2154, 2344
MboII GAAGA 8 cut(s) 88, 89, 118, 148, 1172, 1431, 1684, 1748
MflI RGATCY 1 cut(s) 2007
MhlI GDGCHC 8 cut(s) 243, 1208, 1298, 2080, 2095, 2103, 2169, 2449
MlsI TGGCCA 1 cut(s) 1882
MluNI TGGCCA 1 cut(s) 1882
Mly113I GGCGCC 1 cut(s) 58
MlyI GAGTC 2 cut(s) 755, 1840
MmeI TCCRAC 1 cut(s) 60
Mox20I TGGCCA 1 cut(s) 1882
MroXI GAANNNNTTC 1 cut(s) 1800
MscI TGGCCA 1 cut(s) 1882
MseI TTAA 4 cut(s) 263, 612, 627, 996
MslI CAYNNNNRTG 1 cut(s) 1938
Msp20I TGGCCA 1 cut(s) 1882
MspA1I CMGCKG 6 cut(s) 282, 400, 922, 1228, 1598, 2045
MspCI CTTAAG 1 cut(s) 262
MspR9I CCNGG 9 cut(s) 164, 672, 959, 1175, 1216, 1293, 2074, 2096, 2187
Mva1269I GAATGC 2 cut(s) 1392, 1841
MvaI CCWGG 3 cut(s) 672, 2074, 2187
MvnI CGCG 2 cut(s) 119, 1598
NarI GGCGCC 1 cut(s) 58
NciI CCSGG 6 cut(s) 164, 959, 1175, 1216, 1293, 2096
NcoI CCATGG 2 cut(s) 356, 1954
NdeII GATC 8 cut(s) 13, 345, 565, 738, 1579, 2007, 2154, 2344
NlaIII CATG 8 cut(s) 360, 431, 1257, 1822, 1853, 1912, 1958, 2038
NmuCI GTSAC 2 cut(s) 196, 2300
NspI RCATGY 1 cut(s) 1822
NspV TTCGAA 1 cut(s) 1307
PaeR7I CTCGAG 1 cut(s) 1854
PagI TCATGA 1 cut(s) 2034
PaqCI CACCTGC 1 cut(s) 325
PauI GCGCGC 1 cut(s) 117
PciSI GCTCTTC 2 cut(s) 1185, 1414
PcsI WCGNNNNNNNCGW 2 cut(s) 243, 2262
PctI GAATGC 2 cut(s) 1392, 1841
PdmI GAANNNNTTC 1 cut(s) 1800
PfeI GAWTC 6 cut(s) 315, 378, 925, 1027, 1514, 1753
PflFI GACNNNGTC 1 cut(s) 188
PfoI TCCNGGA 1 cut(s) 957
PleI GAGTC 2 cut(s) 754, 1839
PluTI GGCGCC 1 cut(s) 61
PpsI GAGTC 2 cut(s) 754, 1839
PpuMI RGGWCCY 1 cut(s) 1784
Psp124BI GAGCTC 4 cut(s) 243, 1208, 2103, 2169
Psp5II RGGWCCY 1 cut(s) 1784
Psp6I CCWGG 3 cut(s) 670, 2072, 2185
PspFI CCCAGC 1 cut(s) 306
PspGI CCWGG 3 cut(s) 670, 2072, 2185
PspOMI GGGCCC 1 cut(s) 1294
PspPI GGNCC 8 cut(s) 166, 309, 663, 1294, 1295, 1556, 1784, 1859
PspPPI RGGWCCY 1 cut(s) 1784
PspXI VCTCGAGB 1 cut(s) 1854
PstI CTGCAG 3 cut(s) 405, 1036, 2388
PstNI CAGNNNCTG 3 cut(s) 409, 2045, 2383
PsuI RGATCY 1 cut(s) 2007
PsyI GACNNNGTC 1 cut(s) 188
PteI GCGCGC 1 cut(s) 117
PvuII CAGCTG 3 cut(s) 400, 922, 1228
RsaI GTAC 5 cut(s) 470, 1238, 1330, 1545, 2087
RsaNI GTAC 5 cut(s) 469, 1237, 1329, 1544, 2086
RseI CAYNNNNRTG 1 cut(s) 1938
SacI GAGCTC 4 cut(s) 243, 1208, 2103, 2169
SacII CCGCGG 1 cut(s) 1599
SapI GCTCTTC 2 cut(s) 1185, 1414
SaqAI TTAA 4 cut(s) 263, 612, 627, 996
Sau3AI GATC 8 cut(s) 13, 345, 565, 738, 1579, 2007, 2154, 2344
Sau96I GGNCC 8 cut(s) 166, 309, 663, 1294, 1295, 1556, 1784, 1859
SchI GAGTC 2 cut(s) 755, 1840
ScrFI CCNGG 9 cut(s) 164, 672, 959, 1175, 1216, 1293, 2074, 2096, 2187
SduI GDGCHC 8 cut(s) 243, 1208, 1298, 2080, 2095, 2103, 2169, 2449
SfaNI GCATC 1 cut(s) 1436
SfcI CTRYAG 3 cut(s) 401, 1032, 2384
SfoI GGCGCC 1 cut(s) 59
Sfr274I CTCGAG 1 cut(s) 1854
Sfr303I CCGCGG 1 cut(s) 1599
SfuI TTCGAA 1 cut(s) 1307
SgrBI CCGCGG 1 cut(s) 1599
SinI GGWCC 2 cut(s) 663, 1784
SlaI CTCGAG 1 cut(s) 1854
SmiMI CAYNNNNRTG 1 cut(s) 1938
SmlI CTYRAG 4 cut(s) 262, 1000, 1854, 2111
SmoI CTYRAG 4 cut(s) 262, 1000, 1854, 2111
SspDI GGCGCC 1 cut(s) 57
SspI AATATT 1 cut(s) 1978
SspMI CTAG 6 cut(s) 584, 891, 929, 2081, 2327, 2393
SstI GAGCTC 4 cut(s) 243, 1208, 2103, 2169
StyD4I CCNGG 9 cut(s) 162, 670, 957, 1173, 1214, 1291, 2072, 2094, 2185
StyI CCWWGG 3 cut(s) 356, 1954, 2146
TaaI ACNGT 3 cut(s) 1241, 1630, 2204
TaiI ACGT 2 cut(s) 694, 1731
TauI GCSGC 7 cut(s) 206, 306, 832, 1320, 2045, 2218, 2420
TfiI GAWTC 6 cut(s) 315, 378, 925, 1027, 1514, 1753
Tru1I TTAA 4 cut(s) 263, 612, 627, 996
Tru9I TTAA 4 cut(s) 263, 612, 627, 996
TscAI CASTG 5 cut(s) 1096, 1246, 1674, 2225, 2325
TseFI GTSAC 2 cut(s) 196, 2300
Tsp45I GTSAC 2 cut(s) 196, 2300
TspDTI ATGAA 4 cut(s) 834, 1137, 2213, 2363
TspGWI ACGGA 4 cut(s) 303, 1454, 1733, 2451
TspRI CASTG 5 cut(s) 1096, 1246, 1674, 2225, 2325
Tth111I GACNNNGTC 1 cut(s) 188
Vha464I CTTAAG 1 cut(s) 262
VpaK11BI GGWCC 2 cut(s) 663, 1784
XapI RAATTY 4 cut(s) 846, 1098, 1341, 1709
XbaI TCTAGA 1 cut(s) 928
XceI RCATGY 1 cut(s) 1822
XcmI CCANNNNNNNNNTGG 1 cut(s) 51
XhoI CTCGAG 1 cut(s) 1854
XmiI GTMKAC 1 cut(s) 1632
XmnI GAANNNNTTC 1 cut(s) 1800
XspI CTAG 6 cut(s) 584, 891, 929, 2081, 2327, 2393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.