Rroxscaffold_6G00418060
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0003416
Physical Location & Seq
Reverse (-)
39612365 .. 39615355
2991 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00418060.1

Sequence Viewer

Length: 2445 bp
ATGGACAATAAGTTCATCCATGCCTTACTCTTTGTTCAGCTACTGATTCTCACTTGTAGACCTGTTTCGGGAGGAGGGGATGGAGTAGCTGTCTCCCGAGCAGATTATCTAGCACTTCGAGCATTCAAGCAAGAGCTTATCGATTTCAAAGGCTTATTGCGCAGCTGGAATGACAGTGGCTATGGAGTTTGCTCAGGTGGGTGGGCAGGAATCAAGTGTGTCAAGGGGCAGGTCATTGCAATCCAGCTTCCATGGAAGAGACTTGGGGGGCGAATCTCCGAGAAGATTGGGCAGCTTCAAGGGCTTCGAAAGCTTAGCGTGCATGACAATGTCTTAGGTGGCTCTGTCCCTGCGGCTCTGGGATTCCTCCCCAATCTGAGAGGGGTTTACCTCTTCAATAACCGGCTTTCGGGTTCTGTCCCTGCGTCAATCGGTAACTGTCCTCTTCTTCAAACTCTTGATTTGAGCAACAATGCACTTAGTGGTTCTATTCCTAGTCTTTCAAACTCCACTAGGTTGCTTAGGCTTAATGTGAGCTTCAATTCGCTTTCTGGTTCAATCCCAAGTAGTCTCACTAGGTCAAGTTCCCTAATTTTCCTTGCTCTACAACACAACAACCTTTCTGGTTCTATTCCAAGTACTTGGGGTGGAACAAACAGAACTTGCCAGCTTAAGTTCTTGACCCTAGACAATAATCTCATCTCTGGAACTATCCCAAGCTCTCTGAGCAAGTTGGGTTTTCTTGAAGAGATTTCTCTGAGCAATAACCAGATTACAGGAACCATACCCAATGAAATAGGGGAACTCACAAGGCTCCAAAAGCTAGACCTATCCAACAATGCCATCAATGGAAGCATTCCTGCTACCTTTTCCAATCTGTCCTCCATTGTGACATTGAATCTAGAAGGCAATCGCCTTGATAACCAAATCCCAGAAGTCTTGGAAAGAATGCAGAACCTCTCAGTACTTAATCTGAGGAGTAATAAATTCAGTGGTCATATTCCAGCTTCTATTGGGAATATCTCTGGGATTAACCAAGTTGATTTATCCGAAAACAATTTCACTGGAGAAATTCCTGCTTCACTTTCCAGCCTAGCCAATCTCAATTCCTTCAATGTTTCTTACAACAATCTGTCTGGCTTAGTCCCATCTCTCCTATCCAAAAAGTTCAATTCCAGCTCTTTTGTGGGGAATCTTCAGCTATGTGGGTATAGTGCTTCAACTAGATGTTCTTCTCCGCCATCTCAGAATATTACACTTCCAACGAAAGAGCCTTTGAAGAAAAAGCATCACCACAGACTGAGTACAAAGGACATAATTCTCATAGCAGCAGGTGTCGTCCTAGCGGTTCTGCTTCTACTCTGCTGTTCTTTGCTTCTATGTTTGATCAGGAAAAGGTCTGTATTGAAAGGAAAAAATAGTAAAACCGCCAAGGAACCGGCTGCTGGGAGCATTGACAAGGCAGTTCCTGCAGGCGCTGCGGTTTCATCTGGAGGCGAAGCTGGTGGGAAGCTTGTTCACTTTGATGGGCCGTTTGTGTTTACTGCTGATGATCTATTGTGTGCCACTGCCGAGATAATGGGGAAAAGTACATATGGGACAGCATACAAGGCAACATTGGAGGAGGGTAATCAAGTTGCAGTGAAGAGGTTGAGGGAAAAGACTACAAAGGGTCACAAGGAGTTCGAAACTGAAGCTGCAGCAATTGGGAAGATTCGGCACCCGAATCTCCTTGCTCTTAGGGCCTATTACTTGGGTCCCAAGGGAGAGAAGCTCCTTGTCTTTGATTTCATGCCTAAGGGTAGTCTTGCATCATTCCTTCATGCTCGCGGACCAGAAATGATCGTTGATTGGCCAACAAGGATGAACATAGCCATTGGAGTCACAAGAGGACTATGCCACCTCCACGACCAGGAGAACATCATACATGGGAATCTCACATCCAGCAACATACTACTTGATGAGCAGACCAACGCTCACATTGCAGACTATGGCCTTTCGAGCCTCATGACCCCCGCTGCCAACACCAATGTAATTGCCACTGCAGGAACCCTCGGCTACAACGCGCCAGAGCTCTCAAAGACCAAGAAGAGCACAGAAAAGACTGATGTCTACAGCCTTGGGGTAATCATATTGGAGCTCCTAACAGGAAAATCACCCGGAGAGCCAATGAATGGCATGGACTTGCCGCAGTGGGTGGCGTCGATTGTTAAAGAGGAGTGGACTAATGAAGTTTTTGATTTGGAGCTTATAAGGGATGTTCCCATCATAGGTGATCAGCTGCTTAACACACTGAAATTGTCTCTGCATTGTGTGGATCCCTCACCGGCCGCAAGACCAGAGGCTCAGCAAGTTCTGCAGCAGCTAGAAGAGATTAAGCCGCCTGAGGTAAATGTTGGTTCTGCTGACGAAGGAACAGGAGTACCAGCATCAGCAACTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

814

Amino Acids

87.13

Weight (kDa)

8.62

Isoelectric Point (pI)

35.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 34 - 73 1.1e-06 Leucine rich repeat N-terminal domain
LRR_8 PF13855 101 - 160 4.5e-06 Leucine rich repeat
LRR_4 PF12799 124 - 168 6.3e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 220 - 377 2.1e-11 Leucine-rich repeat region
LRR_8 PF13855 223 - 282 3.7e-08 Leucine rich repeat
LRR_4 PF12799 248 - 282 2.2e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 269 - 306 5.9e-07 Leucine rich repeat
Pkinase PF00069 524 - 788 8.5e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 525 - 789 2.4e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2252
AarI CACCTGC 1 cut(s) 1322
Acc16I TGCGCA 1 cut(s) 161
Acc36I ACCTGC 2 cut(s) 220, 1322
AccB1I GGYRCC 1 cut(s) 1719
AccB7I CCANNNNNTGG 1 cut(s) 2174
AccI GTMKAC 2 cut(s) 58, 2112
AccII CGCG 2 cut(s) 1830, 2066
AclWI GGATC 2 cut(s) 2312, 2325
AcoI YGGCCR 2 cut(s) 1853, 2328
AcsI RAATTY 2 cut(s) 986, 1071
AcuI CTGAAG 2 cut(s) 1181, 1713
AcyI GRCGYC 1 cut(s) 2201
AdeI CACNNNGTG 1 cut(s) 482
AfaI GTAC 5 cut(s) 640, 966, 1306, 1591, 2424
AfiI CCNNNNNNNGG 6 cut(s) 68, 409, 1445, 1912, 2174, 2270
AflII CTTAAG 1 cut(s) 671
AjnI CCWGG 1 cut(s) 1911
AloI GAACNNNNNNTCC 1 cut(s) 28
Alw21I GWGCWC 3 cut(s) 2076, 2096, 2142
Alw26I GTCTC 4 cut(s) 97, 253, 575, 2307
AlwI GGATC 2 cut(s) 2312, 2325
AlwNI CAGNNNCTG 4 cut(s) 43, 1469, 1478, 2438
Ama87I CYCGRG 1 cut(s) 96
AoxI GGCC 5 cut(s) 1529, 1743, 1853, 1993, 2328
ApoI RAATTY 2 cut(s) 986, 1071
AspLEI GCGC 3 cut(s) 162, 1478, 2068
AspS9I GGNCC 4 cut(s) 1529, 1743, 1757, 1832
AsuC2I CCSGG 1 cut(s) 2160
AsuHPI GGTGA 4 cut(s) 1283, 2148, 2285, 2316
AsuII TTCGAA 2 cut(s) 307, 1686
AvaI CYCGRG 1 cut(s) 96
AvaII GGWCC 2 cut(s) 1757, 1832
AxyI CCTNAGG 2 cut(s) 1797, 2385
BaeI ACNNNNGTAYC 2 cut(s) 2406, 2439
BalI TGGCCA 1 cut(s) 1855
BamHI GGATCC 1 cut(s) 2317
BanI GGYRCC 1 cut(s) 1719
BanII GRGCYC 2 cut(s) 2076, 2142
Bbv12I GWGCWC 3 cut(s) 2076, 2096, 2142
BccI CCATC 6 cut(s) 74, 851, 1156, 1249, 1520, 2273
BceAI ACGGC 1 cut(s) 1516
BciT130I CCWGG 1 cut(s) 1913
BclI TGATCA 2 cut(s) 1386, 2275
BcnI CCSGG 1 cut(s) 2160
BcoDI GTCTC 4 cut(s) 97, 253, 575, 2307
BfmI CTRYAG 5 cut(s) 1470, 1698, 2043, 2113, 2357
BfoI RGCGCY 1 cut(s) 1479
BfrI CTTAAG 1 cut(s) 671
BfuAI ACCTGC 2 cut(s) 220, 1322
BlpI GCTNAGC 2 cut(s) 314, 2346
BmcAI AGTACT 2 cut(s) 640, 966
Bme1390I CCNGG 2 cut(s) 1913, 2160
Bme18I GGWCC 2 cut(s) 1757, 1832
BmeT110I CYCGRG 1 cut(s) 96
BmgT120I GGNCC 4 cut(s) 1529, 1743, 1757, 1832
BmiI GGNNCC 8 cut(s) 781, 815, 1437, 1721, 1758, 1759, 2050, 2319
BmrFI CCNGG 2 cut(s) 1913, 2160
BmsI GCATC 3 cut(s) 1297, 1820, 2438
BoxI GACNNNNGTC 1 cut(s) 2108
BplI GAGNNNNNCTC 2 cut(s) 1758, 1790
BpmI CTGGAG 2 cut(s) 1086, 1512
Bpu10I CCTNAGC 2 cut(s) 193, 521
Bpu1102I GCTNAGC 2 cut(s) 314, 2346
Bpu14I TTCGAA 2 cut(s) 307, 1686
BpuMI CCSGG 1 cut(s) 2160
Bsa29I ATCGAT 1 cut(s) 141
BsaHI GRCGYC 1 cut(s) 2201
BsaJI CCNNGG 5 cut(s) 251, 1431, 1761, 2053, 2119
BsaXI ACNNNNNCTCC 2 cut(s) 75, 105
Bsc4I CCNNNNNNNGG 6 cut(s) 68, 409, 1445, 1912, 2174, 2270
Bse118I RCCGGY 3 cut(s) 402, 1438, 2326
Bse1I ACTGG 1 cut(s) 1069
Bse21I CCTNAGG 2 cut(s) 1797, 2385
Bse3DI GCAATG 2 cut(s) 234, 1980
BseBI CCWGG 1 cut(s) 1913
BseCI ATCGAT 1 cut(s) 141
BseDI CCNNGG 5 cut(s) 251, 1431, 1761, 2053, 2119
BseGI GGATG 5 cut(s) 15, 85, 1869, 1940, 2263
BseLI CCNNNNNNNGG 6 cut(s) 68, 409, 1445, 1912, 2174, 2270
BseMI GCAATG 2 cut(s) 234, 1980
BseNI ACTGG 1 cut(s) 1069
BseRI GAGGAG 4 cut(s) 87, 991, 1637, 2231
BseX3I CGGCCG 1 cut(s) 2328
BseYI CCCAGC 1 cut(s) 1445
Bsh1236I CGCG 2 cut(s) 1830, 2066
Bsh1285I CGRYCG 1 cut(s) 2331
BshFI GGCC 5 cut(s) 1531, 1745, 1855, 1995, 2330
BshNI GGYRCC 1 cut(s) 1719
BshVI ATCGAT 1 cut(s) 141
BsiEI CGRYCG 1 cut(s) 2331
BsiHKAI GWGCWC 3 cut(s) 2076, 2096, 2142
BsiHKCI CYCGRG 1 cut(s) 96
BsiSI CCGG 4 cut(s) 403, 1439, 2160, 2327
BslFI GGGAC 5 cut(s) 332, 404, 1130, 1612, 1743
BslI CCNNNNNNNGG 6 cut(s) 68, 409, 1445, 1912, 2174, 2270
BsmAI GTCTC 4 cut(s) 97, 253, 575, 2307
BsmFI GGGAC 5 cut(s) 332, 404, 1130, 1612, 1743
BsmI GAATGC 3 cut(s) 122, 855, 954
BsnI GGCC 5 cut(s) 1531, 1745, 1855, 1995, 2330
BsoBI CYCGRG 1 cut(s) 96
Bsp119I TTCGAA 2 cut(s) 307, 1686
Bsp1286I GDGCHC 3 cut(s) 2076, 2096, 2142
Bsp143I GATC 5 cut(s) 1386, 1552, 1842, 2275, 2317
Bsp1720I GCTNAGC 2 cut(s) 314, 2346
Bsp19I CCATGG 1 cut(s) 251
BspANI GGCC 5 cut(s) 1531, 1745, 1855, 1995, 2330
BspDI ATCGAT 1 cut(s) 141
BspFNI CGCG 2 cut(s) 1830, 2066
BspHI TCATGA 1 cut(s) 2007
BspLI GGNNCC 8 cut(s) 781, 815, 1437, 1721, 1758, 1759, 2050, 2319
BspMAI CTGCAG 4 cut(s) 1474, 1702, 2047, 2361
BspMI ACCTGC 2 cut(s) 220, 1322
BspPI GGATC 2 cut(s) 2312, 2325
BspQI GCTCTTC 1 cut(s) 2084
BspT104I TTCGAA 2 cut(s) 307, 1686
BspT107I GGYRCC 1 cut(s) 1719
BspTI CTTAAG 1 cut(s) 671
BsrDI GCAATG 2 cut(s) 234, 1980
BsrFI RCCGGY 3 cut(s) 402, 1438, 2326
BsrI ACTGG 1 cut(s) 1069
BssAI RCCGGY 3 cut(s) 402, 1438, 2326
BssECI CCNNGG 5 cut(s) 251, 1431, 1761, 2053, 2119
BssMI GATC 5 cut(s) 1386, 1552, 1842, 2275, 2317
BssNI GRCGYC 1 cut(s) 2201
BssT1I CCWWGG 4 cut(s) 251, 1431, 1761, 2119
Bst2UI CCWGG 1 cut(s) 1913
Bst4CI ACNGT 2 cut(s) 176, 440
Bst6I CTCTTC 7 cut(s) 251, 398, 450, 741, 1640, 2084, 2364
BstACI GRCGYC 1 cut(s) 2201
BstAFI CTTAAG 1 cut(s) 671
BstAPI GCANNNNNTGC 3 cut(s) 1469, 1478, 2356
BstBI TTCGAA 2 cut(s) 307, 1686
BstC8I GCNNGC 4 cut(s) 320, 668, 1474, 1828
BstDSI CCRYGG 1 cut(s) 251
BstF5I GGATG 5 cut(s) 15, 85, 1869, 1940, 2263
BstFNI CGCG 2 cut(s) 1830, 2066
BstH2I RGCGCY 1 cut(s) 1479
BstHHI GCGC 3 cut(s) 162, 1478, 2068
BstKTI GATC 5 cut(s) 1389, 1555, 1845, 2278, 2320
BstMAI GTCTC 4 cut(s) 97, 253, 575, 2307
BstMBI GATC 5 cut(s) 1386, 1552, 1842, 2275, 2317
BstMCI CGRYCG 1 cut(s) 2331
BstNI CCWGG 1 cut(s) 1913
BstPAI GACNNNNGTC 1 cut(s) 2108
BstSCI CCNGG 2 cut(s) 1911, 2158
BstSFI CTRYAG 5 cut(s) 1470, 1698, 2043, 2113, 2357
BstUI CGCG 2 cut(s) 1830, 2066
BstX2I RGATCY 1 cut(s) 2317
BstXI CCANNNNNNTGG 1 cut(s) 642
BstYI RGATCY 1 cut(s) 2317
BstZI CGGCCG 1 cut(s) 2328
Bsu15I ATCGAT 1 cut(s) 141
Bsu36I CCTNAGG 2 cut(s) 1797, 2385
BsuRI GGCC 5 cut(s) 1531, 1745, 1855, 1995, 2330
BsuTUI ATCGAT 1 cut(s) 141
BtgI CCRYGG 1 cut(s) 251
BtsCI GGATG 5 cut(s) 15, 85, 1869, 1940, 2263
BtsI GCAGTG 4 cut(s) 1566, 1647, 2040, 2198
BtsIMutI CAGTG 8 cut(s) 181, 997, 1062, 1566, 1647, 2040, 2198, 2291
BveI ACCTGC 2 cut(s) 220, 1322
Cac8I GCNNGC 4 cut(s) 320, 668, 1474, 1828
CaiI CAGNNNCTG 4 cut(s) 43, 1469, 1478, 2438
CciI TCATGA 1 cut(s) 2007
CfoI GCGC 3 cut(s) 162, 1478, 2068
Cfr10I RCCGGY 3 cut(s) 402, 1438, 2326
Cfr13I GGNCC 4 cut(s) 1529, 1743, 1757, 1832
ClaI ATCGAT 1 cut(s) 141
CseI GACGC 2 cut(s) 414, 2190
Csp6I GTAC 5 cut(s) 639, 965, 1305, 1590, 2423
CviAII CATG 8 cut(s) 20, 252, 323, 1792, 1823, 1928, 2008, 2179
CviQI GTAC 5 cut(s) 639, 965, 1305, 1590, 2423
DpnI GATC 5 cut(s) 1388, 1554, 1844, 2277, 2319
DpnII GATC 5 cut(s) 1386, 1552, 1842, 2275, 2317
DraIII CACNNNGTG 1 cut(s) 482
EaeI YGGCCR 2 cut(s) 1853, 2328
EagI CGGCCG 1 cut(s) 2328
Eam1104I CTCTTC 7 cut(s) 251, 398, 450, 741, 1640, 2084, 2364
EarI CTCTTC 7 cut(s) 251, 398, 450, 741, 1640, 2084, 2364
EciI GGCGGA 1 cut(s) 1227
Ecl136II GAGCTC 2 cut(s) 2074, 2140
EclXI CGGCCG 1 cut(s) 2328
Eco130I CCWWGG 4 cut(s) 251, 1431, 1761, 2119
Eco24I GRGCYC 2 cut(s) 2076, 2142
Eco47I GGWCC 2 cut(s) 1757, 1832
Eco52I CGGCCG 1 cut(s) 2328
Eco53kI GAGCTC 2 cut(s) 2074, 2140
Eco57I CTGAAG 2 cut(s) 1181, 1713
Eco81I CCTNAGG 2 cut(s) 1797, 2385
Eco88I CYCGRG 1 cut(s) 96
EcoICRI GAGCTC 2 cut(s) 2074, 2140
EcoO109I RGGNCCY 2 cut(s) 1743, 1757
EcoRII CCWGG 1 cut(s) 1911
EcoT14I CCWWGG 4 cut(s) 251, 1431, 1761, 2119
EcoT38I GRGCYC 2 cut(s) 2076, 2142
ErhI CCWWGG 4 cut(s) 251, 1431, 1761, 2119
FaeI CATG 8 cut(s) 23, 255, 326, 1795, 1826, 1931, 2011, 2182
FaqI GGGAC 5 cut(s) 332, 404, 1130, 1612, 1743
FatI CATG 8 cut(s) 19, 251, 322, 1791, 1822, 1927, 2007, 2178
FauI CCCGC 1 cut(s) 2023
FauNDI CATATG 1 cut(s) 1594
FbaI TGATCA 2 cut(s) 1386, 2275
FblI GTMKAC 2 cut(s) 58, 2112
FokI GGATG 5 cut(s) 2, 92, 1876, 1927, 2270
FriOI GRGCYC 2 cut(s) 2076, 2142
FspI TGCGCA 1 cut(s) 161
GlaI GCGC 3 cut(s) 161, 1477, 2067
GsaI CCCAGC 1 cut(s) 1449
GsuI CTGGAG 2 cut(s) 1086, 1512
HaeII RGCGCY 1 cut(s) 1479
HaeIII GGCC 5 cut(s) 1531, 1745, 1855, 1995, 2330
HapII CCGG 4 cut(s) 403, 1439, 2160, 2327
HgaI GACGC 2 cut(s) 414, 2190
HhaI GCGC 3 cut(s) 162, 1478, 2068
Hin1I GRCGYC 1 cut(s) 2201
Hin1II CATG 8 cut(s) 23, 255, 326, 1795, 1826, 1931, 2011, 2182
Hin6I GCGC 3 cut(s) 160, 1476, 2066
HinP1I GCGC 3 cut(s) 160, 1476, 2066
HindIII AAGCTT 2 cut(s) 311, 1511
HpaII CCGG 4 cut(s) 403, 1439, 2160, 2327
HphI GGTGA 4 cut(s) 1283, 2148, 2285, 2316
Hpy166II GTNNAC 6 cut(s) 59, 388, 1519, 1542, 2113, 2223
Hpy188I TCNGA 7 cut(s) 280, 378, 726, 759, 975, 1051, 1248
Hpy8I GTNNAC 6 cut(s) 59, 388, 1519, 1542, 2113, 2223
Hpy99I CGWCG 1 cut(s) 2206
HpyAV CCTTC 4 cut(s) 899, 1120, 1829, 2405
HpyCH4III ACNGT 2 cut(s) 176, 440
Hsp92I GRCGYC 1 cut(s) 2201
Hsp92II CATG 8 cut(s) 23, 255, 326, 1795, 1826, 1931, 2011, 2182
HspAI GCGC 3 cut(s) 160, 1476, 2066
KflI GGGWCCC 1 cut(s) 1757
Ksp22I TGATCA 2 cut(s) 1386, 2275
Kzo9I GATC 5 cut(s) 1386, 1552, 1842, 2275, 2317
LguI GCTCTTC 1 cut(s) 2084
LmnI GCTCC 6 cut(s) 819, 1449, 1779, 2137, 2145, 2245
LweI GCATC 3 cut(s) 1297, 1820, 2438
MaeIII GTNAC 4 cut(s) 434, 889, 1673, 1882
MalI GATC 5 cut(s) 1388, 1554, 1844, 2277, 2319
MboI GATC 5 cut(s) 1386, 1552, 1842, 2275, 2317
MfeI CAATTG 1 cut(s) 1704
MflI RGATCY 1 cut(s) 2317
MhlI GDGCHC 3 cut(s) 2076, 2096, 2142
MlsI TGGCCA 1 cut(s) 1855
MluNI TGGCCA 1 cut(s) 1855
MlyI GAGTC 1 cut(s) 1890
MmeI TCCRAC 2 cut(s) 858, 1286
Mox20I TGGCCA 1 cut(s) 1855
MscI TGGCCA 1 cut(s) 1855
MseI TTAA 7 cut(s) 528, 672, 969, 1032, 2211, 2286, 2376
MslI CAYNNNNRTG 2 cut(s) 327, 1524
Msp20I TGGCCA 1 cut(s) 1855
MspA1I CMGCKG 3 cut(s) 165, 2018, 2281
MspCI CTTAAG 1 cut(s) 671
MspI CCGG 4 cut(s) 403, 1439, 2160, 2327
MspR9I CCNGG 2 cut(s) 1913, 2160
MunI CAATTG 1 cut(s) 1704
Mva1269I GAATGC 3 cut(s) 122, 855, 954
MvaI CCWGG 1 cut(s) 1913
MvnI CGCG 2 cut(s) 1830, 2066
NciI CCSGG 1 cut(s) 2160
NcoI CCATGG 1 cut(s) 251
NdeI CATATG 1 cut(s) 1594
NdeII GATC 5 cut(s) 1386, 1552, 1842, 2275, 2317
NlaIII CATG 8 cut(s) 23, 255, 326, 1795, 1826, 1931, 2011, 2182
NlaIV GGNNCC 8 cut(s) 781, 815, 1437, 1721, 1758, 1759, 2050, 2319
NmeAIII GCCGAG 2 cut(s) 1597, 2034
NmuCI GTSAC 3 cut(s) 889, 1673, 1882
NsbI TGCGCA 1 cut(s) 161
NspV TTCGAA 2 cut(s) 307, 1686
PagI TCATGA 1 cut(s) 2007
PaqCI CACCTGC 1 cut(s) 1322
PciSI GCTCTTC 1 cut(s) 2084
PctI GAATGC 3 cut(s) 122, 855, 954
PfeI GAWTC 9 cut(s) 46, 210, 273, 363, 898, 1192, 1714, 1726, 1933
PflFI GACNNNGTC 1 cut(s) 329
PflMI CCANNNNNTGG 1 cut(s) 2174
PleI GAGTC 1 cut(s) 1889
PpsI GAGTC 1 cut(s) 1889
PpuMI RGGWCCY 1 cut(s) 1757
PshAI GACNNNNGTC 1 cut(s) 2108
PsiI TTATAA 1 cut(s) 2252
Psp124BI GAGCTC 2 cut(s) 2076, 2142
Psp5II RGGWCCY 1 cut(s) 1757
Psp6I CCWGG 1 cut(s) 1911
PspFI CCCAGC 1 cut(s) 1445
PspGI CCWGG 1 cut(s) 1911
PspN4I GGNNCC 8 cut(s) 781, 815, 1437, 1721, 1758, 1759, 2050, 2319
PspPI GGNCC 4 cut(s) 1529, 1743, 1757, 1832
PspPPI RGGWCCY 1 cut(s) 1757
PstI CTGCAG 4 cut(s) 1474, 1702, 2047, 2361
PstNI CAGNNNCTG 4 cut(s) 43, 1469, 1478, 2438
PsuI RGATCY 1 cut(s) 2317
PsyI GACNNNGTC 1 cut(s) 329
PvuII CAGCTG 2 cut(s) 165, 2281
RsaI GTAC 5 cut(s) 640, 966, 1306, 1591, 2424
RsaNI GTAC 5 cut(s) 639, 965, 1305, 1590, 2423
RseI CAYNNNNRTG 2 cut(s) 327, 1524
SacI GAGCTC 2 cut(s) 2076, 2142
SapI GCTCTTC 1 cut(s) 2084
SaqAI TTAA 7 cut(s) 528, 672, 969, 1032, 2211, 2286, 2376
Sau3AI GATC 5 cut(s) 1386, 1552, 1842, 2275, 2317
Sau96I GGNCC 4 cut(s) 1529, 1743, 1757, 1832
SbfI CCTGCAGG 1 cut(s) 1474
ScaI AGTACT 2 cut(s) 640, 966
SchI GAGTC 1 cut(s) 1890
ScrFI CCNGG 2 cut(s) 1913, 2160
SdaI CCTGCAGG 1 cut(s) 1474
SduI GDGCHC 3 cut(s) 2076, 2096, 2142
SfaNI GCATC 3 cut(s) 1297, 1820, 2438
SfcI CTRYAG 5 cut(s) 1470, 1698, 2043, 2113, 2357
SfuI TTCGAA 2 cut(s) 307, 1686
SinI GGWCC 2 cut(s) 1757, 1832
SmiMI CAYNNNNRTG 2 cut(s) 327, 1524
SmlI CTYRAG 1 cut(s) 671
SmoI CTYRAG 1 cut(s) 671
Sse8387I CCTGCAGG 1 cut(s) 1474
SspI AATATT 1 cut(s) 1252
SstI GAGCTC 2 cut(s) 2076, 2142
StyD4I CCNGG 2 cut(s) 1911, 2158
StyI CCWWGG 4 cut(s) 251, 1431, 1761, 2119
TaaI ACNGT 2 cut(s) 176, 440
TaqI TCGA 6 cut(s) 118, 141, 307, 1686, 2000, 2204
TatI WGTACW 4 cut(s) 638, 964, 1304, 1589
TauI GCSGC 4 cut(s) 356, 2191, 2333, 2383
TfiI GAWTC 9 cut(s) 46, 210, 273, 363, 898, 1192, 1714, 1726, 1933
Tru1I TTAA 7 cut(s) 528, 672, 969, 1032, 2211, 2286, 2376
Tru9I TTAA 7 cut(s) 528, 672, 969, 1032, 2211, 2286, 2376
TscAI CASTG 8 cut(s) 181, 997, 1069, 1573, 1647, 2047, 2198, 2298
TseFI GTSAC 3 cut(s) 889, 1673, 1882
Tsp45I GTSAC 3 cut(s) 889, 1673, 1882
TspDTI ATGAA 8 cut(s) 4, 807, 1476, 1780, 1811, 1880, 2186, 2244
TspRI CASTG 8 cut(s) 181, 997, 1069, 1573, 1647, 2047, 2198, 2298
Tth111I GACNNNGTC 1 cut(s) 329
Van91I CCANNNNNTGG 1 cut(s) 2174
Vha464I CTTAAG 1 cut(s) 671
VpaK11BI GGWCC 2 cut(s) 1757, 1832
XapI RAATTY 2 cut(s) 986, 1071
XbaI TCTAGA 1 cut(s) 901
XcmI CCANNNNNNNNNTGG 1 cut(s) 1183
XmiI GTMKAC 2 cut(s) 58, 2112
ZrmI AGTACT 2 cut(s) 640, 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.