Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0003416

Physical Location & Seq

Genomic Coordinates Reverse (-)

60556840 .. 60558583

Gene Structure 1744 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ59535

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1278 bp

ATGTTCTCAATCCAGTGGACAATGAAGCTATGTGGCTACAGTGCTTTAACTTCATGCCCTTCTGAAGCACCTTCTCAAAGTGGTGTAGCTCCAGCACCTGAGGTAGCAAAACGTCGCCGACGCAGACTTAGTACCAAAGACAGAATTCTCATAGCAGCAGCAGTACTTCTAGTAGTTTTGTTTTTACTTTGCTGCATACTACTTTGCTGTTTGATCCGAAAAAGAGCTGCATCAAAATCAAAGGATGGTGGGGTGGCTGGGGCAGGTGCTGCTAGAACCGAAAAGGGGGTTCCGGTTGGTGGGGTTGAAATCGAATCTGGTGGAGAGGCAGGAGGAAAACTAGTGCATTTTGATGGGCCGATGGCTTTTACAGCAGATGATCTTTTGTGTGCAACAGCAGAGATTATGGGGAAAAGCACTTTCGGGACAGTCTACAAGGCCACACTGGAGGATGGGAGTGAAGTTGCAGTGAAGAGATTGAGAGAGAAGATTACTAAGAATCAGAGGGAGTTCGAAGCAGAAGTGAATATACTTGGGAAAATCAGACATCCAAACCTGTTGGCTTTGAGGGCTTATTACTTGGGACCTAAAGGAGAAAAGCTTCTGGTTTTCGATTACATGCCTAAAGGAAGTCTTGCTGCATTTCTCCATGCTCGTGGACCTGATACACCGATTGATTGGCCAACAAGAATGAGAATCGCAAAGGGCATGGCGCGAGGGCTGTCCTATCTCCACAGCAATGAGAACATCATACATGGGAACCTTACATCCAGCAATGTCCTACTTGATGAGCAAACTGATTCTAAGATTGCAGATTATGGTCTCTCTAAGCTTATGACAGCTGCTGCCAATTCCAATGTGATTGCAACAGCTGGGGCACTAGGGTACCGAGCACCCGAGCTCTCAAAGCTCAAGAAGGCCAACACGAAAACTGATGTGTACAGCCTTGGTGTGATCATATTGGAACTCCTAACAGGAAAGTCTCCCGGCGAGCCAATGAACGGCTTGGATTTGCCTCAGTGGGTTGCCTCCATTGTCAAAGAGGAGTGGACGAACGAAGTTTTTGACCTGGAATTGATGAGGGACGCGTCTGTCATCGGGAATGAGTTGCTAAACACATTGAAACTGGCTTTGCACTGTGTTGATCCTTCCCCATCAGCAAGACCGGAAGTTCTGCAGGTTCTGCAGCAACTCGAAGAGATTAGACCCGAAACAGCTGCCAGTTCAGCCGATGATGGAGGTGCAGAACCCTCAATCCCTTCAACAACAAGCGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

425

Amino Acids

45.66

Weight (kDa)

7.01

Isoelectric Point (pI)

40.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	133 - 398	1.1e-42	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	133 - 396	8.8e-40	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003416)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51740
fragaria_vesca	FvH4_6g10640 FvH4_6g10640 FvH4_7g25350
malus_domestica	MD01G1159600.v1.1 MD04G1166300.v1.1 MD04G1166400.v1.1 MD07G1228100.v1.1
prunus_persica	Prupe.2G257600_v2.0.a1 Prupe.6G284600_v2.0.a1
pyrus_communis	pycom04g14780 pycom07g20650 pycom12g16950
rosa_chinensis	RchiOBHm_Chr1g0371261 RchiOBHm_Chr1g0371271 RchiOBHm_Chr3g0461661
rosa_laevigata	RLG00000024909 RLG00000026975
rosa_multiflora	Rmu_sc0001978.1_g000006 Rmu_sc0012406.1_g000007
rosa_roxburghii	Rroxscaffold_4G00285830 Rroxscaffold_6G00418060
rosa_rugosa	Rorug01G0362500 Rorug03G0052700
rosa_samantha	Rh1AG372600 Rh1BG336100 Rh1CG349500 Rh1DG367200 Rh3AG111000 Rh3AG111100 Rh3BG114000 Rh3CG116000 Rh3CG116100 Rh3DG115900
rosa_wichuraiana	Rw1G032770 Rw3G009390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	254
AasI	GACNNNNNNGTC	1 cut(s)	1091
Acc36I	ACCTGC	2 cut(s)	254, 1168
Acc65I	GGTACC	1 cut(s)	885
AccB1I	GGYRCC	1 cut(s)	885
AccI	GTMKAC	1 cut(s)	432
AccII	CGCG	2 cut(s)	715, 1088
AclWI	GGATC	2 cut(s)	208, 1139
AcoI	YGGCCR	1 cut(s)	680
AcsI	RAATTY	1 cut(s)	144
AcuI	CTGAAG	1 cut(s)	84
AfaI	GTAC	4 cut(s)	133, 165, 887, 941
AfiI	CCNNNNNNNGG	3 cut(s)	285, 299, 1001
AflIII	ACRYGT	1 cut(s)	1086
AgsI	TTSAA	3 cut(s)	308, 1123, 1263
AhlI	ACTAGT	1 cut(s)	340
AjnI	CCWGG	1 cut(s)	1068
AloI	GAACNNNNNNTCC	4 cut(s)	752, 784, 1239, 1271
Alw21I	GWGCWC	2 cut(s)	895, 903
Alw26I	GTCTC	2 cut(s)	827, 987
AlwI	GGATC	2 cut(s)	208, 1139
AlwNI	CAGNNNCTG	4 cut(s)	98, 269, 845, 1183
Ama87I	CYCGRG	1 cut(s)	896
AoxI	GGCC	4 cut(s)	356, 438, 680, 918
ApoI	RAATTY	1 cut(s)	144
Asp700I	GAANNNNTTC	1 cut(s)	600
Asp718I	GGTACC	1 cut(s)	885
AspLEI	GCGC	1 cut(s)	715
AspS9I	GGNCC	3 cut(s)	356, 584, 659
AsuC2I	CCSGG	1 cut(s)	987
AsuII	TTCGAA	1 cut(s)	513
AvaI	CYCGRG	1 cut(s)	896
AvaII	GGWCC	2 cut(s)	584, 659
AxyI	CCTNAGG	1 cut(s)	99
BaeGI	GKGCMC	1 cut(s)	880
BaeI	ACNNNNGTAYC	2 cut(s)	869, 902
BalI	TGGCCA	1 cut(s)	682
BanI	GGYRCC	1 cut(s)	885
BanII	GRGCYC	1 cut(s)	903
BarI	GAAGNNNNNNTAC	2 cut(s)	513, 545
BauI	CACGAG	1 cut(s)	654
Bbv12I	GWGCWC	2 cut(s)	895, 903
BccI	CCATC	6 cut(s)	239, 347, 355, 446, 1162, 1229
BceAI	ACGGC	1 cut(s)	1018
BcgI	CGANNNNNNTGC	2 cut(s)	1199, 1233
BciT130I	CCWGG	1 cut(s)	1070
BclI	TGATCA	1 cut(s)	954
BcnI	CCSGG	1 cut(s)	987
BcoDI	GTCTC	2 cut(s)	827, 987
BcuI	ACTAGT	1 cut(s)	340
BfaI	CTAG	4 cut(s)	170, 273, 341, 881
BfmI	CTRYAG	3 cut(s)	37, 1175, 1184
BfuAI	ACCTGC	2 cut(s)	254, 1168
BmcAI	AGTACT	1 cut(s)	165
Bme1390I	CCNGG	2 cut(s)	987, 1070
Bme18I	GGWCC	2 cut(s)	584, 659
BmeT110I	CYCGRG	1 cut(s)	896
BmgT120I	GGNCC	3 cut(s)	356, 584, 659
BmiI	GGNNCC	4 cut(s)	291, 585, 761, 887
BmrFI	CCNGG	2 cut(s)	987, 1070
BmsI	GCATC	1 cut(s)	239
BpmI	CTGGAG	2 cut(s)	75, 467
Bpu14I	TTCGAA	1 cut(s)	513
BpuEI	CTTGAG	1 cut(s)	896
BpuMI	CCSGG	1 cut(s)	987
BsaI	GGTCTC	1 cut(s)	827
BsaJI	CCNNGG	1 cut(s)	946
BsaWI	WCCGGW	2 cut(s)	292, 1165
Bsc4I	CCNNNNNNNGG	3 cut(s)	285, 299, 1001
Bse1I	ACTGG	4 cut(s)	13, 450, 1131, 1221
Bse21I	CCTNAGG	1 cut(s)	99
Bse3DI	GCAATG	2 cut(s)	745, 781
BseBI	CCWGG	1 cut(s)	1070
BseDI	CCNNGG	1 cut(s)	946
BseGI	GGATG	4 cut(s)	250, 457, 547, 767
BseLI	CCNNNNNNNGG	3 cut(s)	285, 299, 1001
BseMI	GCAATG	2 cut(s)	745, 781
BseMII	CTCAG	2 cut(s)	90, 1031
BseNI	ACTGG	4 cut(s)	13, 450, 1131, 1221
BseRI	GAGGAG	1 cut(s)	1058
BseSI	GKGCMC	1 cut(s)	880
BseYI	CCCAGC	2 cut(s)	257, 872
BsgI	GTGCAG	1 cut(s)	1263
Bsh1236I	CGCG	2 cut(s)	715, 1088
BshFI	GGCC	4 cut(s)	358, 440, 682, 920
BshNI	GGYRCC	1 cut(s)	885
BsiHKAI	GWGCWC	2 cut(s)	895, 903
BsiHKCI	CYCGRG	1 cut(s)	896
BsiSI	CCGG	3 cut(s)	293, 987, 1166
BslFI	GGGAC	3 cut(s)	439, 597, 1097
BslI	CCNNNNNNNGG	3 cut(s)	285, 299, 1001
BsmAI	GTCTC	2 cut(s)	827, 987
BsmFI	GGGAC	3 cut(s)	439, 597, 1097
BsnI	GGCC	4 cut(s)	358, 440, 682, 920
Bso31I	GGTCTC	1 cut(s)	827
BsoBI	CYCGRG	1 cut(s)	896
Bsp119I	TTCGAA	1 cut(s)	513
Bsp1286I	GDGCHC	3 cut(s)	880, 895, 903
Bsp1407I	TGTACA	1 cut(s)	939
Bsp143I	GATC	4 cut(s)	213, 379, 954, 1144
BspANI	GGCC	4 cut(s)	358, 440, 682, 920
BspCNI	CTCAG	2 cut(s)	91, 1030
BspFNI	CGCG	2 cut(s)	715, 1088
BspLI	GGNNCC	4 cut(s)	291, 585, 761, 887
BspMAI	CTGCAG	2 cut(s)	1179, 1188
BspMI	ACCTGC	2 cut(s)	254, 1168
BspPI	GGATC	2 cut(s)	208, 1139
BspT104I	TTCGAA	1 cut(s)	513
BspT107I	GGYRCC	1 cut(s)	885
BspTNI	GGTCTC	1 cut(s)	827
BsrDI	GCAATG	2 cut(s)	745, 781
BsrGI	TGTACA	1 cut(s)	939
BsrI	ACTGG	4 cut(s)	13, 450, 1131, 1221
BssECI	CCNNGG	1 cut(s)	946
BssMI	GATC	4 cut(s)	213, 379, 954, 1144
BssSI	CACGAG	1 cut(s)	654
BssT1I	CCWWGG	1 cut(s)	946
Bst2BI	CACGAG	1 cut(s)	654
Bst2UI	CCWGG	1 cut(s)	1070
Bst4CI	ACNGT	3 cut(s)	41, 430, 1139
Bst6I	CTCTTC	2 cut(s)	467, 1191
BstAPI	GCANNNNNTGC	2 cut(s)	269, 1183
BstAUI	TGTACA	1 cut(s)	939
BstBI	TTCGAA	1 cut(s)	513
BstC8I	GCNNGC	1 cut(s)	992
BstDEI	CTNAG	6 cut(s)	99, 128, 495, 804, 828, 1017
BstF5I	GGATG	4 cut(s)	250, 457, 547, 767
BstFNI	CGCG	2 cut(s)	715, 1088
BstHHI	GCGC	1 cut(s)	715
BstKTI	GATC	4 cut(s)	216, 382, 957, 1147
BstMAI	GTCTC	2 cut(s)	827, 987
BstMBI	GATC	4 cut(s)	213, 379, 954, 1144
BstMWI	GCNNNNNNNGC	6 cut(s)	269, 371, 569, 907, 1183, 1226
BstNI	CCWGG	1 cut(s)	1070
BstNSI	RCATGY	1 cut(s)	622
BstSCI	CCNGG	2 cut(s)	985, 1068
BstSFI	CTRYAG	3 cut(s)	37, 1175, 1184
BstSLI	GKGCMC	1 cut(s)	880
BstUI	CGCG	2 cut(s)	715, 1088
BstXI	CCANNNNNNTGG	1 cut(s)	656
Bsu36I	CCTNAGG	1 cut(s)	99
BsuRI	GGCC	4 cut(s)	358, 440, 682, 920
BtsCI	GGATG	4 cut(s)	250, 457, 547, 767
BtsI	GCAGTG	1 cut(s)	474
BtsIMutI	CAGTG	6 cut(s)	20, 46, 443, 474, 1025, 1135
BveI	ACCTGC	2 cut(s)	254, 1168
Cac8I	GCNNGC	1 cut(s)	992
CaiI	CAGNNNCTG	4 cut(s)	98, 269, 845, 1183
CfoI	GCGC	1 cut(s)	715
Cfr13I	GGNCC	3 cut(s)	356, 584, 659
CseI	GACGC	3 cut(s)	129, 1077, 1094
Csp6I	GTAC	4 cut(s)	132, 164, 886, 940
CviAII	CATG	5 cut(s)	54, 619, 650, 709, 755
CviQI	GTAC	4 cut(s)	132, 164, 886, 940
DdeI	CTNAG	6 cut(s)	99, 128, 495, 804, 828, 1017
DpnI	GATC	4 cut(s)	215, 381, 956, 1146
DpnII	GATC	4 cut(s)	213, 379, 954, 1144
DrdI	GACNNNNNNGTC	1 cut(s)	1091
DseDI	GACNNNNNNGTC	1 cut(s)	1091
EaeI	YGGCCR	1 cut(s)	680
Eam1104I	CTCTTC	2 cut(s)	467, 1191
EarI	CTCTTC	2 cut(s)	467, 1191
Ecl136II	GAGCTC	1 cut(s)	901
Eco130I	CCWWGG	1 cut(s)	946
Eco24I	GRGCYC	1 cut(s)	903
Eco31I	GGTCTC	1 cut(s)	827
Eco47I	GGWCC	2 cut(s)	584, 659
Eco53kI	GAGCTC	1 cut(s)	901
Eco57I	CTGAAG	1 cut(s)	84
Eco81I	CCTNAGG	1 cut(s)	99
Eco88I	CYCGRG	1 cut(s)	896
EcoICRI	GAGCTC	1 cut(s)	901
EcoO109I	RGGNCCY	1 cut(s)	584
EcoRI	GAATTC	1 cut(s)	144
EcoRII	CCWGG	1 cut(s)	1068
EcoT14I	CCWWGG	1 cut(s)	946
EcoT38I	GRGCYC	1 cut(s)	903
ErhI	CCWWGG	1 cut(s)	946
FaeI	CATG	5 cut(s)	57, 622, 653, 712, 758
FalI	AAGNNNNNCTT	2 cut(s)	618, 650
FaqI	GGGAC	3 cut(s)	439, 597, 1097
FatI	CATG	5 cut(s)	53, 618, 649, 708, 754
FbaI	TGATCA	1 cut(s)	954
FblI	GTMKAC	1 cut(s)	432
FokI	GGATG	4 cut(s)	257, 464, 534, 754
FriOI	GRGCYC	1 cut(s)	903
FspBI	CTAG	4 cut(s)	170, 273, 341, 881
GlaI	GCGC	1 cut(s)	714
GsaI	CCCAGC	2 cut(s)	261, 876
GsuI	CTGGAG	2 cut(s)	75, 467
HaeIII	GGCC	4 cut(s)	358, 440, 682, 920
HapII	CCGG	3 cut(s)	293, 987, 1166
HgaI	GACGC	3 cut(s)	129, 1077, 1094
HhaI	GCGC	1 cut(s)	715
Hin1II	CATG	5 cut(s)	57, 622, 653, 712, 758
Hin6I	GCGC	1 cut(s)	713
HinP1I	GCGC	1 cut(s)	713
HindIII	AAGCTT	2 cut(s)	599, 830
HinfI	GANTC	4 cut(s)	314, 499, 696, 800
HpaII	CCGG	3 cut(s)	293, 987, 1166
Hpy166II	GTNNAC	5 cut(s)	18, 433, 659, 940, 1050
Hpy188I	TCNGA	4 cut(s)	64, 218, 504, 545
Hpy188III	TCNNGA	3 cut(s)	424, 913, 1099
Hpy8I	GTNNAC	5 cut(s)	18, 433, 659, 940, 1050
Hpy99I	CGWCG	2 cut(s)	117, 123
HpyAV	CCTTC	5 cut(s)	69, 81, 910, 1158, 1269
HpyCH4III	ACNGT	3 cut(s)	41, 430, 1139
HpyCH4IV	ACGT	1 cut(s)	112
HpyF10VI	GCNNNNNNNGC	6 cut(s)	269, 371, 569, 907, 1183, 1226
HpyF3I	CTNAG	6 cut(s)	99, 128, 495, 804, 828, 1017
HpySE526I	ACGT	1 cut(s)	112
Hsp92II	CATG	5 cut(s)	57, 622, 653, 712, 758
HspAI	GCGC	1 cut(s)	713
KpnI	GGTACC	1 cut(s)	889
Ksp22I	TGATCA	1 cut(s)	954
Kzo9I	GATC	4 cut(s)	213, 379, 954, 1144
LmnI	GCTCC	1 cut(s)	94
LweI	GCATC	1 cut(s)	239
MaeI	CTAG	4 cut(s)	170, 273, 341, 881
MaeII	ACGT	1 cut(s)	112
MalI	GATC	4 cut(s)	215, 381, 956, 1146
MboI	GATC	4 cut(s)	213, 379, 954, 1144
MboII	GAAGA	3 cut(s)	484, 499, 1208
MhlI	GDGCHC	3 cut(s)	880, 895, 903
MlsI	TGGCCA	1 cut(s)	682
MluCI	AATT	3 cut(s)	144, 850, 1073
MluI	ACGCGT	1 cut(s)	1086
MluNI	TGGCCA	1 cut(s)	682
Mox20I	TGGCCA	1 cut(s)	682
MroXI	GAANNNNTTC	1 cut(s)	600
MscI	TGGCCA	1 cut(s)	682
MseI	TTAA	1 cut(s)	47
MslI	CAYNNNNRTG	3 cut(s)	351, 654, 738
Msp20I	TGGCCA	1 cut(s)	682
MspA1I	CMGCKG	3 cut(s)	842, 872, 1217
MspI	CCGG	3 cut(s)	293, 987, 1166
MspR9I	CCNGG	2 cut(s)	987, 1070
MvaI	CCWGG	1 cut(s)	1070
MvnI	CGCG	2 cut(s)	715, 1088
MwoI	GCNNNNNNNGC	6 cut(s)	269, 371, 569, 907, 1183, 1226
NciI	CCSGG	1 cut(s)	987
NdeII	GATC	4 cut(s)	213, 379, 954, 1144
NlaIII	CATG	5 cut(s)	57, 622, 653, 712, 758
NlaIV	GGNNCC	4 cut(s)	291, 585, 761, 887
NspI	RCATGY	1 cut(s)	622
NspV	TTCGAA	1 cut(s)	513
PaqCI	CACCTGC	1 cut(s)	254
PdmI	GAANNNNTTC	1 cut(s)	600
PfeI	GAWTC	4 cut(s)	314, 499, 696, 800
PpuMI	RGGWCCY	1 cut(s)	584
Psp124BI	GAGCTC	1 cut(s)	903
Psp5II	RGGWCCY	1 cut(s)	584
Psp6I	CCWGG	1 cut(s)	1068
PspFI	CCCAGC	2 cut(s)	257, 872
PspGI	CCWGG	1 cut(s)	1068
PspN4I	GGNNCC	4 cut(s)	291, 585, 761, 887
PspPI	GGNCC	3 cut(s)	356, 584, 659
PspPPI	RGGWCCY	1 cut(s)	584
PstI	CTGCAG	2 cut(s)	1179, 1188
PstNI	CAGNNNCTG	4 cut(s)	98, 269, 845, 1183
PvuII	CAGCTG	3 cut(s)	842, 872, 1217
RsaI	GTAC	4 cut(s)	133, 165, 887, 941
RsaNI	GTAC	4 cut(s)	132, 164, 886, 940
RseI	CAYNNNNRTG	3 cut(s)	351, 654, 738
SacI	GAGCTC	1 cut(s)	903
SaqAI	TTAA	1 cut(s)	47
Sau3AI	GATC	4 cut(s)	213, 379, 954, 1144
Sau96I	GGNCC	3 cut(s)	356, 584, 659
ScaI	AGTACT	1 cut(s)	165
ScrFI	CCNGG	2 cut(s)	987, 1070
SduI	GDGCHC	3 cut(s)	880, 895, 903
SfaNI	GCATC	1 cut(s)	239
SfcI	CTRYAG	3 cut(s)	37, 1175, 1184
SfuI	TTCGAA	1 cut(s)	513
SinI	GGWCC	2 cut(s)	584, 659
SmiMI	CAYNNNNRTG	3 cut(s)	351, 654, 738
SmlI	CTYRAG	1 cut(s)	911
SmoI	CTYRAG	1 cut(s)	911
SpeI	ACTAGT	1 cut(s)	340
Sse9I	AATT	3 cut(s)	144, 850, 1073
SspMI	CTAG	4 cut(s)	170, 273, 341, 881
SstI	GAGCTC	1 cut(s)	903
StyD4I	CCNGG	2 cut(s)	985, 1068
StyI	CCWWGG	1 cut(s)	946
TaaI	ACNGT	3 cut(s)	41, 430, 1139
TaiI	ACGT	1 cut(s)	115
TaqI	TCGA	4 cut(s)	312, 513, 612, 1194
TasI	AATT	3 cut(s)	144, 850, 1073
TatI	WGTACW	2 cut(s)	163, 939
TfiI	GAWTC	4 cut(s)	314, 499, 696, 800
Tru1I	TTAA	1 cut(s)	47
Tru9I	TTAA	1 cut(s)	47
TscAI	CASTG	6 cut(s)	20, 46, 450, 474, 1025, 1142
TspDTI	ATGAA	3 cut(s)	38, 42, 1013
TspRI	CASTG	6 cut(s)	20, 46, 450, 474, 1025, 1142
VpaK11BI	GGWCC	2 cut(s)	584, 659
XapI	RAATTY	1 cut(s)	144
XceI	RCATGY	1 cut(s)	622
XmiI	GTMKAC	1 cut(s)	432
XmnI	GAANNNNTTC	1 cut(s)	600
XspI	CTAG	4 cut(s)	170, 273, 341, 881
ZrmI	AGTACT	1 cut(s)	165

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.