MD04G1166400.v1.1
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003416
Physical Location & Seq
Reverse (-)
25673118 .. 25675497
2380 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1166400.v1.1.491

Sequence Viewer

Length: 1926 bp
ATGTTCATGGAAAATAAGTTGTTGTTTGTCATCCATGCCTTGCTCTTTCTTCAGCTATTAATTGTCGCTTACCAGCCTGTTTCGGGCCAGCGAGGGAATGATGGAGTGATTGTGACTCAGTCCGATTACCAAGCTCTTCGAGCATTCAAGCGCGAGCTTGTTGATTTCACCGGTGTACTGCGCAGCTGGAATGACAGCGGACATGGAGCCTGCTTGGGCTGGTGGGCAGGGATCAAGTGTGTGAACGGACAGGTCATTGCAATCCAGCTTCCGTGGAGGAGACTCGGGGGCCGAATATCTGAAAAGATTGGGCAGCTGCAAGCGCTTCGGAAGCTCAGCCTGCACGACAATGTCTTGGCTGGCCCTGTCCCTTTGTCTCTTGGCTTCCTTCCCAATCTCAGAGGGGTTTACCTCTTCAACAACCGGCTTTCGGGTTCTGTCCCGCCTTCGATTGGTAACTGCCCTCTTCTTCAAACTCTTGATTTGAGCAATAATTCGCTTACTGGTACAATCCCTTCTAGTCTTGCAAATTCTACCAAGCTGTACAGACTTAATCTGAGCTTCAATTCGCTTTCGGGTTTCATCCCAACTAGTCTCACCAAGTTCCCTTCTCTCACCATCCTTGCCCTCCAACACAACAACCTCTCTGGTCCTATTCCAAGCACTTGGGGTGCTGCAAATCGAAATCATAGTTACAAACTTACGATCCTGACCCTTGATAATAATCTGATTTCCGGAACTATTCCAAGTTCTCTGAGCAAATTGGGTTTTCTTGAAGAGATTTATTTGAACAACAACCAGATTTCCGGGACCATTCCCGATGAAATAGGGGAGCTCACAAGGCTCCAAAAGCTAGACTTATCCAACAATGCCATCAATGGCAGCTTCCCTTCTAGCTTCTCCAACCTCTCCTCCCTTGTTTCGTTGAGCCTCGAGGGCAACCGCCTCAATAACCAAATCCCGGAAGGCCTGGAAAGGTTGCAGAACCTCTCAGTACTTAACTTGAAGAAAAACAATTTCATGGGCAAAATTCCTGCTTCACTTTCCAGCTTAGACAACCTCACTTCCTTCAATGTTTCTCACAACAATCTCTCTGGCCCCGTCCCTTCTCTCCTCTCGAAAAAGTTCAATTCTAGCTCTTTTGCGGGCAATCTTCAGCTGTGCGGGTACAGCGCTTCAACTCCGTGTTCTTCTCCCCCGCCTCAGATTCTTCCATCTTCTCCGCCAGCAGAGCAGCCCTTGAAGAAAAAGCATCACCACAAATTTAGTACGAAGGACAAAATTCTCATAGCGGCGGGTGCCCTCCTGGCGGTTCTGCTTCTACTCTGCTGCATTTTGCTTGTTTGTTTGGTGAAAAGGTCTGCTTCAAAAGGAATGAATGGTACAACGGCTAAGCAGGCCGCTGATGCGGGGATCGCTGCCAAGGCAGTTCCTGGTAGTGCCGGAGTTGAATATGGCGGTGAAGCTGGTGGGAAGCTTGTTCACTTTGATGGGCCTTTCGTTTTCACAGCTGATGACCTGCTGTGTGCCACTGCTGAAATAATGGGGAAGAGCACATATGGGACTGCATACAAGGCAACATTAGAGGAAGGACATGAAGTTGCAGTGAAGAGATTGAGGGAAAAGACAACGAAAGGTCAGAAGGAATTCGAAACTGAAGCTGCAGCAATTGGGAAGATTCGCCACCCGAATCTCTTGGCTCTGAGGGCTTATTACTTGGGACCTAAGGGAGAGAAGCTTCTCGTCTTCGATTACATGTCTAAGGGTAGCCTTGCATCCTTCCTTCATGCTCGAGGGCCAGACACCATCATTGATTGGCCAACAAGGATGAACATAGCGATCTGGCATCACTCGGGGACTATGCTATCTCCACAACGACGAGAACATTGTACATGGCAATCTCACATCCAGCAACATTCTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

642

Amino Acids

69.46

Weight (kDa)

9.44

Isoelectric Point (pI)

37.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 40 - 80 5.5e-07 Leucine rich repeat N-terminal domain
LRR_4 PF12799 131 - 169 2.7e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 156 - 215 7.5e-09 Leucine rich repeat
LRR_14 PF23598 199 - 363 1.8e-11 Leucine-rich repeat region
LRR_8 PF13855 233 - 292 9.3e-09 Leucine rich repeat
LRR_8 PF13855 278 - 316 1.1e-07 Leucine rich repeat
Pkinase PF00069 512 - 598 4.3e-08 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 513 - 606 3e-12 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 932
Acc16I TGCGCA 1 cut(s) 182
Acc36I ACCTGC 1 cut(s) 1527
AccB1I GGYRCC 1 cut(s) 1298
AccII CGCG 1 cut(s) 153
AccIII TCCGGA 1 cut(s) 734
AclWI GGATC 3 cut(s) 239, 700, 1421
AcoI YGGCCR 1 cut(s) 1817
AcsI RAATTY 5 cut(s) 529, 1029, 1262, 1281, 1646
AcuI CTGAAG 3 cut(s) 35, 1139, 1677
AfaI GTAC 8 cut(s) 177, 508, 545, 996, 1169, 1270, 1384, 1891
AfeI AGCGCT 2 cut(s) 324, 1174
AfiI CCNNNNNNNGG 5 cut(s) 83, 430, 452, 961, 1309
AflIII ACRYGT 1 cut(s) 1755
AgeI ACCGGT 1 cut(s) 170
AhlI ACTAGT 1 cut(s) 590
AjnI CCWGG 3 cut(s) 969, 1305, 1432
Alw21I GWGCWC 2 cut(s) 837, 1556
Alw26I GTCTC 3 cut(s) 274, 381, 599
AlwI GGATC 3 cut(s) 239, 700, 1421
AlwNI CAGNNNCTG 1 cut(s) 1433
Ama87I CYCGRG 4 cut(s) 284, 932, 1791, 1852
Aor13HI TCCGGA 1 cut(s) 734
Aor51HI AGCGCT 2 cut(s) 324, 1174
AoxI GGCC 9 cut(s) 85, 289, 361, 967, 1096, 1398, 1493, 1796, 1817
ApoI RAATTY 5 cut(s) 529, 1029, 1262, 1281, 1646
AseI ATTAAT 1 cut(s) 59
AsiGI ACCGGT 1 cut(s) 170
Asp700I GAANNNNTTC 2 cut(s) 1124, 1646
AspLEI GCGC 4 cut(s) 153, 183, 325, 1175
AspS9I GGNCC 9 cut(s) 85, 289, 362, 650, 810, 1097, 1493, 1721, 1796
AsuC2I CCSGG 2 cut(s) 808, 962
AsuHPI GGTGA 6 cut(s) 160, 589, 607, 1247, 1363, 1472
AsuII TTCGAA 1 cut(s) 1650
AvaI CYCGRG 4 cut(s) 284, 932, 1791, 1852
AvaII GGWCC 3 cut(s) 650, 810, 1721
AxyI CCTNAGG 1 cut(s) 1725
BaeGI GKGCMC 1 cut(s) 1303
BalI TGGCCA 1 cut(s) 1819
BanI GGYRCC 1 cut(s) 1298
BanII GRGCYC 1 cut(s) 837
BarI GAAGNNNNNNTAC 2 cut(s) 499, 531
BbsI GAAGAC 1 cut(s) 1738
Bbv12I GWGCWC 2 cut(s) 837, 1556
BccI CCATC 6 cut(s) 95, 626, 881, 1222, 1484, 1814
BceAI ACGGC 1 cut(s) 1404
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BciT130I CCWGG 3 cut(s) 971, 1307, 1434
BcnI CCSGG 2 cut(s) 808, 962
BcoDI GTCTC 3 cut(s) 274, 381, 599
BcuI ACTAGT 1 cut(s) 590
BfaI CTAG 5 cut(s) 519, 591, 854, 894, 1134
BfmI CTRYAG 1 cut(s) 1662
BfoI RGCGCY 2 cut(s) 326, 1176
BfuAI ACCTGC 1 cut(s) 1527
BglI GCCNNNNNGGC 2 cut(s) 936, 1307
BlpI GCTNAGC 2 cut(s) 335, 1392
BmcAI AGTACT 1 cut(s) 996
Bme1390I CCNGG 5 cut(s) 808, 962, 971, 1307, 1434
Bme18I GGWCC 3 cut(s) 650, 810, 1721
BmeT110I CYCGRG 4 cut(s) 284, 932, 1791, 1852
BmgT120I GGNCC 9 cut(s) 85, 289, 362, 650, 810, 1097, 1493, 1721, 1796
BmiI GGNNCC 7 cut(s) 208, 290, 811, 845, 1099, 1300, 1722
BmrFI CCNGG 5 cut(s) 808, 962, 971, 1307, 1434
BmsI GCATC 4 cut(s) 1261, 1396, 1784, 1855
BpiI GAAGAC 1 cut(s) 1738
Bpu1102I GCTNAGC 2 cut(s) 335, 1392
Bpu14I TTCGAA 1 cut(s) 1650
BpuMI CCSGG 2 cut(s) 808, 962
BsaBI GATNNNNATC 1 cut(s) 723
BsaJI CCNNGG 2 cut(s) 272, 1422
BsaWI WCCGGW 2 cut(s) 170, 734
BsaXI ACNNNNNCTCC 4 cut(s) 96, 126, 896, 926
Bsc4I CCNNNNNNNGG 5 cut(s) 83, 430, 452, 961, 1309
Bse118I RCCGGY 2 cut(s) 170, 423
Bse1I ACTGG 1 cut(s) 508
Bse21I CCTNAGG 1 cut(s) 1725
Bse3DI GCAATG 1 cut(s) 255
Bse8I GATNNNNATC 1 cut(s) 723
BseAI TCCGGA 1 cut(s) 734
BseBI CCWGG 3 cut(s) 971, 1307, 1434
BseDI CCNNGG 2 cut(s) 272, 1422
BseGI GGATG 6 cut(s) 30, 582, 618, 1775, 1833, 1905
BseJI GATNNNNATC 1 cut(s) 723
BseLI CCNNNNNNNGG 5 cut(s) 83, 430, 452, 961, 1309
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 8 cut(s) 131, 349, 412, 548, 746, 1005, 1217, 1694
BseNI ACTGG 1 cut(s) 508
BseRI GAGGAG 3 cut(s) 292, 901, 1103
BseSI GKGCMC 1 cut(s) 1303
BsgI GTGCAG 1 cut(s) 326
Bsh1236I CGCG 1 cut(s) 153
BshFI GGCC 9 cut(s) 87, 291, 363, 969, 1098, 1400, 1495, 1798, 1819
BshNI GGYRCC 1 cut(s) 1298
BshTI ACCGGT 1 cut(s) 170
BsiHKAI GWGCWC 2 cut(s) 837, 1556
BsiHKCI CYCGRG 4 cut(s) 284, 932, 1791, 1852
BsiSI CCGG 6 cut(s) 171, 424, 735, 807, 962, 1443
BslFI GGGAC 7 cut(s) 353, 425, 823, 1088, 1576, 1734, 1870
BslI CCNNNNNNNGG 5 cut(s) 83, 430, 452, 961, 1309
BsmAI GTCTC 3 cut(s) 274, 381, 599
BsmFI GGGAC 7 cut(s) 353, 425, 823, 1088, 1576, 1734, 1870
BsmI GAATGC 1 cut(s) 143
BsnI GGCC 9 cut(s) 87, 291, 363, 969, 1098, 1400, 1495, 1798, 1819
BsoBI CYCGRG 4 cut(s) 284, 932, 1791, 1852
Bsp119I TTCGAA 1 cut(s) 1650
Bsp1286I GDGCHC 3 cut(s) 837, 1303, 1556
Bsp13I TCCGGA 1 cut(s) 734
Bsp1407I TGTACA 2 cut(s) 543, 1889
Bsp143I GATC 4 cut(s) 231, 705, 1413, 1839
Bsp1720I GCTNAGC 2 cut(s) 335, 1392
BspANI GGCC 9 cut(s) 87, 291, 363, 969, 1098, 1400, 1495, 1798, 1819
BspCNI CTCAG 8 cut(s) 130, 348, 411, 549, 747, 1004, 1216, 1695
BspEI TCCGGA 1 cut(s) 734
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 7 cut(s) 208, 290, 811, 845, 1099, 1300, 1722
BspMAI CTGCAG 1 cut(s) 1666
BspMI ACCTGC 1 cut(s) 1527
BspPI GGATC 3 cut(s) 239, 700, 1421
BspQI GCTCTTC 2 cut(s) 141, 1544
BspT104I TTCGAA 1 cut(s) 1650
BspT107I GGYRCC 1 cut(s) 1298
BsrDI GCAATG 1 cut(s) 255
BsrFI RCCGGY 2 cut(s) 170, 423
BsrGI TGTACA 2 cut(s) 543, 1889
BsrI ACTGG 1 cut(s) 508
BssAI RCCGGY 2 cut(s) 170, 423
BssECI CCNNGG 2 cut(s) 272, 1422
BssMI GATC 4 cut(s) 231, 705, 1413, 1839
BssT1I CCWWGG 1 cut(s) 1422
Bst2UI CCWGG 3 cut(s) 971, 1307, 1434
Bst6I CTCTTC 6 cut(s) 141, 419, 471, 771, 1544, 1604
BstAUI TGTACA 2 cut(s) 543, 1889
BstBI TTCGAA 1 cut(s) 1650
BstC8I GCNNGC 9 cut(s) 89, 155, 211, 321, 341, 361, 1147, 1227, 1398
BstDSI CCRYGG 1 cut(s) 272
BstF5I GGATG 6 cut(s) 30, 582, 618, 1775, 1833, 1905
BstFNI CGCG 1 cut(s) 153
BstH2I RGCGCY 2 cut(s) 326, 1176
BstHHI GCGC 4 cut(s) 153, 183, 325, 1175
BstKTI GATC 4 cut(s) 234, 708, 1416, 1842
BstMAI GTCTC 3 cut(s) 274, 381, 599
BstMBI GATC 4 cut(s) 231, 705, 1413, 1839
BstNI CCWGG 3 cut(s) 971, 1307, 1434
BstNSI RCATGY 1 cut(s) 1759
BstSCI CCNGG 5 cut(s) 806, 960, 969, 1305, 1432
BstSFI CTRYAG 1 cut(s) 1662
BstSLI GKGCMC 1 cut(s) 1303
BstUI CGCG 1 cut(s) 153
BstV2I GAAGAC 1 cut(s) 1738
BstXI CCANNNNNNTGG 1 cut(s) 666
Bsu36I CCTNAGG 1 cut(s) 1725
BsuRI GGCC 9 cut(s) 87, 291, 363, 969, 1098, 1400, 1495, 1798, 1819
BtgI CCRYGG 1 cut(s) 272
BtsCI GGATG 6 cut(s) 30, 582, 618, 1775, 1833, 1905
BtsI GCAGTG 2 cut(s) 1530, 1611
BtsIMutI CAGTG 2 cut(s) 1530, 1611
BveI ACCTGC 1 cut(s) 1527
Cac8I GCNNGC 9 cut(s) 89, 155, 211, 321, 341, 361, 1147, 1227, 1398
CaiI CAGNNNCTG 1 cut(s) 1433
CfoI GCGC 4 cut(s) 153, 183, 325, 1175
Cfr10I RCCGGY 2 cut(s) 170, 423
Cfr13I GGNCC 9 cut(s) 85, 289, 362, 650, 810, 1097, 1493, 1721, 1796
Csp6I GTAC 8 cut(s) 176, 507, 544, 995, 1168, 1269, 1383, 1890
CspAI ACCGGT 1 cut(s) 170
CviAII CATG 8 cut(s) 7, 35, 203, 1021, 1595, 1756, 1787, 1893
CviQI GTAC 8 cut(s) 176, 507, 544, 995, 1168, 1269, 1383, 1890
DpnI GATC 4 cut(s) 233, 707, 1415, 1841
DpnII GATC 4 cut(s) 231, 705, 1413, 1839
EaeI YGGCCR 1 cut(s) 1817
Eam1104I CTCTTC 6 cut(s) 141, 419, 471, 771, 1544, 1604
EarI CTCTTC 6 cut(s) 141, 419, 471, 771, 1544, 1604
EciI GGCGGA 1 cut(s) 1212
Ecl136II GAGCTC 1 cut(s) 835
Eco130I CCWWGG 1 cut(s) 1422
Eco147I AGGCCT 1 cut(s) 969
Eco24I GRGCYC 1 cut(s) 837
Eco47I GGWCC 3 cut(s) 650, 810, 1721
Eco47III AGCGCT 2 cut(s) 324, 1174
Eco53kI GAGCTC 1 cut(s) 835
Eco57I CTGAAG 3 cut(s) 35, 1139, 1677
Eco81I CCTNAGG 1 cut(s) 1725
Eco88I CYCGRG 4 cut(s) 284, 932, 1791, 1852
EcoICRI GAGCTC 1 cut(s) 835
EcoO109I RGGNCCY 1 cut(s) 1721
EcoRI GAATTC 1 cut(s) 1646
EcoRII CCWGG 3 cut(s) 969, 1305, 1432
EcoT14I CCWWGG 1 cut(s) 1422
EcoT38I GRGCYC 1 cut(s) 837
ErhI CCWWGG 1 cut(s) 1422
FaeI CATG 8 cut(s) 10, 38, 206, 1024, 1598, 1759, 1790, 1896
FalI AAGNNNNNCTT 4 cut(s) 842, 874, 1348, 1380
FaqI GGGAC 7 cut(s) 353, 425, 823, 1088, 1576, 1734, 1870
FatI CATG 8 cut(s) 6, 34, 202, 1020, 1594, 1755, 1786, 1892
FauI CCCGC 6 cut(s) 450, 1138, 1157, 1206, 1288, 1402
FauNDI CATATG 1 cut(s) 1558
FokI GGATG 6 cut(s) 17, 569, 605, 1762, 1840, 1892
FriOI GRGCYC 1 cut(s) 837
FspBI CTAG 5 cut(s) 519, 591, 854, 894, 1134
FspI TGCGCA 1 cut(s) 182
GlaI GCGC 4 cut(s) 152, 182, 324, 1174
HaeII RGCGCY 2 cut(s) 326, 1176
HaeIII GGCC 9 cut(s) 87, 291, 363, 969, 1098, 1400, 1495, 1798, 1819
HapII CCGG 6 cut(s) 171, 424, 735, 807, 962, 1443
HhaI GCGC 4 cut(s) 153, 183, 325, 1175
Hin1II CATG 8 cut(s) 10, 38, 206, 1024, 1598, 1759, 1790, 1896
Hin6I GCGC 4 cut(s) 151, 181, 323, 1173
HinP1I GCGC 4 cut(s) 151, 181, 323, 1173
HindIII AAGCTT 2 cut(s) 1475, 1736
HinfI GANTC 5 cut(s) 115, 282, 1207, 1678, 1690
HpaII CCGG 6 cut(s) 171, 424, 735, 807, 962, 1443
HphI GGTGA 6 cut(s) 160, 589, 607, 1247, 1363, 1472
Hpy166II GTNNAC 4 cut(s) 176, 244, 409, 1483
Hpy188III TCNNGA 6 cut(s) 479, 709, 735, 773, 818, 1117
Hpy8I GTNNAC 4 cut(s) 176, 244, 409, 1483
Hpy99I CGWCG 1 cut(s) 1881
Hsp92II CATG 8 cut(s) 10, 38, 206, 1024, 1598, 1759, 1790, 1896
HspAI GCGC 4 cut(s) 151, 181, 323, 1173
Kpn2I TCCGGA 1 cut(s) 734
Kzo9I GATC 4 cut(s) 231, 705, 1413, 1839
LguI GCTCTTC 2 cut(s) 141, 1544
LmnI GCTCC 3 cut(s) 206, 832, 849
LweI GCATC 4 cut(s) 1261, 1396, 1784, 1855
MaeI CTAG 5 cut(s) 519, 591, 854, 894, 1134
MaeIII GTNAC 3 cut(s) 112, 455, 692
MalI GATC 4 cut(s) 233, 707, 1415, 1841
MboI GATC 4 cut(s) 231, 705, 1413, 1839
MfeI CAATTG 1 cut(s) 1668
MhlI GDGCHC 3 cut(s) 837, 1303, 1556
MlsI TGGCCA 1 cut(s) 1819
MluNI TGGCCA 1 cut(s) 1819
MlyI GAGTC 2 cut(s) 109, 276
MmeI TCCRAC 3 cut(s) 655, 888, 927
Mox20I TGGCCA 1 cut(s) 1819
MroI TCCGGA 1 cut(s) 734
MroXI GAANNNNTTC 2 cut(s) 1124, 1646
MscI TGGCCA 1 cut(s) 1819
MseI TTAA 3 cut(s) 59, 552, 999
MslI CAYNNNNRTG 2 cut(s) 348, 1488
Msp20I TGGCCA 1 cut(s) 1819
MspA1I CMGCKG 6 cut(s) 186, 198, 316, 1159, 1403, 1511
MspI CCGG 6 cut(s) 171, 424, 735, 807, 962, 1443
MspR9I CCNGG 5 cut(s) 808, 962, 971, 1307, 1434
MunI CAATTG 1 cut(s) 1668
Mva1269I GAATGC 1 cut(s) 143
MvaI CCWGG 3 cut(s) 971, 1307, 1434
MvnI CGCG 1 cut(s) 153
NciI CCSGG 2 cut(s) 808, 962
NdeI CATATG 1 cut(s) 1558
NdeII GATC 4 cut(s) 231, 705, 1413, 1839
NlaIII CATG 8 cut(s) 10, 38, 206, 1024, 1598, 1759, 1790, 1896
NlaIV GGNNCC 7 cut(s) 208, 290, 811, 845, 1099, 1300, 1722
NmuCI GTSAC 1 cut(s) 112
NsbI TGCGCA 1 cut(s) 182
NspI RCATGY 1 cut(s) 1759
NspV TTCGAA 1 cut(s) 1650
PaeR7I CTCGAG 2 cut(s) 932, 1791
PceI AGGCCT 1 cut(s) 969
PciI ACATGT 1 cut(s) 1755
PciSI GCTCTTC 2 cut(s) 141, 1544
PctI GAATGC 1 cut(s) 143
PdmI GAANNNNTTC 2 cut(s) 1124, 1646
PfeI GAWTC 3 cut(s) 1207, 1678, 1690
PflFI GACNNNGTC 2 cut(s) 118, 350
PfoI TCCNGGA 2 cut(s) 806, 960
PinAI ACCGGT 1 cut(s) 170
PleI GAGTC 2 cut(s) 109, 276
PpsI GAGTC 2 cut(s) 109, 276
PpuMI RGGWCCY 1 cut(s) 1721
PscI ACATGT 1 cut(s) 1755
PshBI ATTAAT 1 cut(s) 59
Psp124BI GAGCTC 1 cut(s) 837
Psp5II RGGWCCY 1 cut(s) 1721
Psp6I CCWGG 3 cut(s) 969, 1305, 1432
PspGI CCWGG 3 cut(s) 969, 1305, 1432
PspN4I GGNNCC 7 cut(s) 208, 290, 811, 845, 1099, 1300, 1722
PspPI GGNCC 9 cut(s) 85, 289, 362, 650, 810, 1097, 1493, 1721, 1796
PspPPI RGGWCCY 1 cut(s) 1721
PspXI VCTCGAGB 2 cut(s) 932, 1791
PstI CTGCAG 1 cut(s) 1666
PstNI CAGNNNCTG 1 cut(s) 1433
PsyI GACNNNGTC 2 cut(s) 118, 350
PvuII CAGCTG 4 cut(s) 186, 316, 1159, 1511
RsaI GTAC 8 cut(s) 177, 508, 545, 996, 1169, 1270, 1384, 1891
RsaNI GTAC 8 cut(s) 176, 507, 544, 995, 1168, 1269, 1383, 1890
RseI CAYNNNNRTG 2 cut(s) 348, 1488
SacI GAGCTC 1 cut(s) 837
SapI GCTCTTC 2 cut(s) 141, 1544
SaqAI TTAA 3 cut(s) 59, 552, 999
Sau3AI GATC 4 cut(s) 231, 705, 1413, 1839
Sau96I GGNCC 9 cut(s) 85, 289, 362, 650, 810, 1097, 1493, 1721, 1796
ScaI AGTACT 1 cut(s) 996
SchI GAGTC 2 cut(s) 109, 276
ScrFI CCNGG 5 cut(s) 808, 962, 971, 1307, 1434
SduI GDGCHC 3 cut(s) 837, 1303, 1556
SfaNI GCATC 4 cut(s) 1261, 1396, 1784, 1855
SfcI CTRYAG 1 cut(s) 1662
Sfr274I CTCGAG 2 cut(s) 932, 1791
SfuI TTCGAA 1 cut(s) 1650
SgrAI CRCCGGYG 1 cut(s) 170
SinI GGWCC 3 cut(s) 650, 810, 1721
SlaI CTCGAG 2 cut(s) 932, 1791
SmiMI CAYNNNNRTG 2 cut(s) 348, 1488
SmlI CTYRAG 2 cut(s) 932, 1791
SmoI CTYRAG 2 cut(s) 932, 1791
SpeI ACTAGT 1 cut(s) 590
SseBI AGGCCT 1 cut(s) 969
SspMI CTAG 5 cut(s) 519, 591, 854, 894, 1134
SstI GAGCTC 1 cut(s) 837
StuI AGGCCT 1 cut(s) 969
StyD4I CCNGG 5 cut(s) 806, 960, 969, 1305, 1432
StyI CCWWGG 1 cut(s) 1422
TaqI TCGA 8 cut(s) 139, 449, 682, 933, 1118, 1650, 1749, 1792
TatI WGTACW 4 cut(s) 175, 543, 994, 1889
TauI GCSGC 2 cut(s) 1295, 1403
TfiI GAWTC 3 cut(s) 1207, 1678, 1690
Tru1I TTAA 3 cut(s) 59, 552, 999
Tru9I TTAA 3 cut(s) 59, 552, 999
TscAI CASTG 2 cut(s) 1537, 1611
TseFI GTSAC 1 cut(s) 112
Tsp45I GTSAC 1 cut(s) 112
TspDTI ATGAA 7 cut(s) 571, 837, 1009, 1391, 1611, 1775, 1844
TspGWI ACGGA 3 cut(s) 261, 261, 1173
TspRI CASTG 2 cut(s) 1537, 1611
Tth111I GACNNNGTC 2 cut(s) 118, 350
VpaK11BI GGWCC 3 cut(s) 650, 810, 1721
VspI ATTAAT 1 cut(s) 59
XapI RAATTY 5 cut(s) 529, 1029, 1262, 1281, 1646
XceI RCATGY 1 cut(s) 1759
XhoI CTCGAG 2 cut(s) 932, 1791
XmnI GAANNNNTTC 2 cut(s) 1124, 1646
XspI CTAG 5 cut(s) 519, 591, 854, 894, 1134
ZrmI AGTACT 1 cut(s) 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.