FvH4_7g25770

alpha-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006739
Physical Location & Seq
Reverse (-)
19648520 .. 19653357
4838 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g25770.t3

Sequence Viewer

Length: 1116 bp
ATGTTCTCGGATTTGCTTGGTTTATGGTGGAATAGCTGGAATTTCTTTGCTTGTGATATTAATGAAACTGTGATCAAGGAAACAGCTGATGCACTCATCTCAACCGGATTAGCTGATCTAGGTTATGTGTATGTCAATATAGATGATTGCTGGTCTATCCCAATACGAAATTCTGAGGGTCAACTTGTCCCTGATCCTAAAACATTTCCATCGGGAATTAAAGCTCTTGCCGATTATATACATGGGAAGGGCCTGAAGCTTGGAATCTATTCTGATGCTGGGGTTTTCACATGTCAAGTTCGACCAGGCTCACTTTATCATGAAAGTGATGATGCAAAGCTGTTTGCTTCTTGGGATGTAGATTATTTGAAGTATGACAACTGTTACAATCTGGGTATCCCACCAAAAGAACGGTACCCTCCCATGCGCGAGGCCTTAAATACATCTGGACGGACAATTTTCTATTCAGTTTGTGAATGGGGTGTTGATGATCCTGCTTTATGGGCCGGGAAGGTTGGGAATAGTTGGCGTACAACAGATGATATTAATGATTCATGGGCAAGCATGACTACAATTGCTGATTTGAATGACAAGTGGGCGTCGTATGCAGGACCTGGTGGATGGAACGATCCGGATATGTTGGAAGTTGGCAATGGAGGCATGAGTTACCAGGAATACCGTGCTCATTTTAGCATCTGGGCTTTGATGAAGGCCCCTCTTTTGATTGGTTGTGATGTTAGAAACATGACTGCAGAAACATACGAGATTCTAAGCAATGAGGAGGTTATTGCTGTAAACCAAGATTCTCTTGGGGTTCAGGGAAGAAAAGTGAATGTTTCAGGAACAGATGGGGTCTATCAGGTTTGGGCAGGTCCTCTATCTGGACATCGTTTGGCTGTTGCTCTTTGGAATCGGGGTTCAACAGCGAAGACTATAACAGTTAAATGGGAAGAACTTGGACTTCAGTCCAGCATTAGTGTTTCAGTGAGAGATTTGTGGCAGCACAAACTAGTTACGGATAATGCCGTATCATCATTTGGTGCTCTGGTTGAGGCTCACGACTGTCACCTGTACCTCTTCACTCCCCAGACTGAGTCATTAAGAAATGTATTTTAG

Protein Analysis

372

Amino Acids

41.27

Weight (kDa)

4.71

Isoelectric Point (pI)

25.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Melibiase_2 PF16499 66 - 328 2.2e-80 Alpha galactosidase A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 860
Acc65I GGTACC 1 cut(s) 414
AccB1I GGYRCC 1 cut(s) 414
AccII CGCG 1 cut(s) 429
AccIII TCCGGA 1 cut(s) 631
AclWI GGATC 3 cut(s) 188, 485, 623
AcsI RAATTY 2 cut(s) 40, 169
AcuI CTGAAG 2 cut(s) 275, 947
AcyI GRCGYC 1 cut(s) 599
AfaI GTAC 3 cut(s) 416, 532, 1073
AfiI CCNNNNNNNGG 1 cut(s) 881
AflIII ACRYGT 1 cut(s) 290
AgsI TTSAA 3 cut(s) 370, 586, 921
AhlI ACTAGT 1 cut(s) 1009
AjnI CCWGG 3 cut(s) 304, 613, 669
AjuI GAANNNNNNNTTGG 2 cut(s) 154, 186
AloI GAACNNNNNNTCC 2 cut(s) 901, 933
AluBI AGCT 6 cut(s) 36, 86, 113, 224, 259, 340
AluI AGCT 6 cut(s) 36, 86, 113, 224, 259, 340
Alw21I GWGCWC 2 cut(s) 685, 1045
AlwI GGATC 3 cut(s) 188, 485, 623
AlwNI CAGNNNCTG 1 cut(s) 614
Aor13HI TCCGGA 1 cut(s) 631
AoxI GGCC 4 cut(s) 250, 432, 504, 711
ApeKI GCWGC 1 cut(s) 1000
ApoI RAATTY 2 cut(s) 40, 169
AseI ATTAAT 2 cut(s) 60, 546
Asp700I GAANNNNTTC 1 cut(s) 268
Asp718I GGTACC 1 cut(s) 414
AspLEI GCGC 1 cut(s) 429
AspS9I GGNCC 5 cut(s) 250, 504, 611, 712, 872
AsuC2I CCSGG 1 cut(s) 508
AsuHPI GGTGA 1 cut(s) 1058
AvaII GGWCC 2 cut(s) 611, 872
BanI GGYRCC 1 cut(s) 414
BbsI GAAGAC 1 cut(s) 935
Bbv12I GWGCWC 2 cut(s) 685, 1045
BbvI GCAGC 1 cut(s) 1012
BccI CCATC 3 cut(s) 217, 615, 842
BceAI ACGGC 1 cut(s) 1010
BciT130I CCWGG 3 cut(s) 306, 615, 671
BciVI GTATCC 1 cut(s) 407
BclI TGATCA 1 cut(s) 72
BcnI CCSGG 1 cut(s) 508
BcuI ACTAGT 1 cut(s) 1009
BfaI CTAG 2 cut(s) 119, 1010
BfmI CTRYAG 1 cut(s) 750
BfuAI ACCTGC 1 cut(s) 860
BfuI GTATCC 1 cut(s) 407
BisI GCNGC 1 cut(s) 1001
BlsI GCNGC 1 cut(s) 1002
Bme1390I CCNGG 4 cut(s) 306, 508, 615, 671
Bme18I GGWCC 2 cut(s) 611, 872
BmgT120I GGNCC 5 cut(s) 250, 504, 611, 712, 872
BmiI GGNNCC 2 cut(s) 416, 714
BmrFI CCNGG 4 cut(s) 306, 508, 615, 671
BmsI GCATC 4 cut(s) 79, 265, 322, 702
BoxI GACNNNNGTC 1 cut(s) 964
BpiI GAAGAC 1 cut(s) 935
BpuMI CCSGG 1 cut(s) 508
BsaHI GRCGYC 1 cut(s) 599
BsaWI WCCGGW 2 cut(s) 104, 631
Bsc4I CCNNNNNNNGG 1 cut(s) 881
Bse3DI GCAATG 2 cut(s) 658, 781
BseAI TCCGGA 1 cut(s) 631
BseBI CCWGG 3 cut(s) 306, 615, 671
BseGI GGATG 2 cut(s) 361, 626
BseLI CCNNNNNNNGG 1 cut(s) 881
BseMI GCAATG 2 cut(s) 658, 781
BseMII CTCAG 2 cut(s) 165, 1083
BseRI GAGGAG 1 cut(s) 794
BseXI GCAGC 1 cut(s) 1012
BseYI CCCAGC 1 cut(s) 278
Bsh1236I CGCG 1 cut(s) 429
BshFI GGCC 4 cut(s) 252, 434, 506, 713
BshNI GGYRCC 1 cut(s) 414
BsiHKAI GWGCWC 2 cut(s) 685, 1045
BsiSI CCGG 3 cut(s) 105, 507, 632
BslFI GGGAC 1 cut(s) 173
BslI CCNNNNNNNGG 1 cut(s) 881
BsmFI GGGAC 1 cut(s) 173
BsnI GGCC 4 cut(s) 252, 434, 506, 713
Bsp1286I GDGCHC 2 cut(s) 685, 1045
Bsp13I TCCGGA 1 cut(s) 631
Bsp143I GATC 5 cut(s) 72, 115, 193, 490, 628
BspANI GGCC 4 cut(s) 252, 434, 506, 713
BspCNI CTCAG 2 cut(s) 166, 1084
BspEI TCCGGA 1 cut(s) 631
BspFNI CGCG 1 cut(s) 429
BspHI TCATGA 1 cut(s) 319
BspLI GGNNCC 2 cut(s) 416, 714
BspMAI CTGCAG 1 cut(s) 754
BspMI ACCTGC 1 cut(s) 860
BspPI GGATC 3 cut(s) 188, 485, 623
BspT107I GGYRCC 1 cut(s) 414
BsrDI GCAATG 2 cut(s) 658, 781
BssMI GATC 5 cut(s) 72, 115, 193, 490, 628
BssNI GRCGYC 1 cut(s) 599
Bst2UI CCWGG 3 cut(s) 306, 615, 671
Bst4CI ACNGT 6 cut(s) 70, 383, 414, 680, 940, 1064
Bst6I CTCTTC 1 cut(s) 1082
BstACI GRCGYC 1 cut(s) 599
BstC8I GCNNGC 1 cut(s) 562
BstDEI CTNAG 3 cut(s) 174, 770, 1092
BstF5I GGATG 2 cut(s) 361, 626
BstFNI CGCG 1 cut(s) 429
BstHHI GCGC 1 cut(s) 429
BstKTI GATC 5 cut(s) 75, 118, 196, 493, 631
BstMBI GATC 5 cut(s) 72, 115, 193, 490, 628
BstMWI GCNNNNNNNGC 3 cut(s) 503, 605, 657
BstNI CCWGG 3 cut(s) 306, 615, 671
BstNSI RCATGY 1 cut(s) 294
BstPAI GACNNNNGTC 1 cut(s) 964
BstSCI CCNGG 4 cut(s) 304, 506, 613, 669
BstSFI CTRYAG 1 cut(s) 750
BstUI CGCG 1 cut(s) 429
BstV1I GCAGC 1 cut(s) 1012
BstV2I GAAGAC 1 cut(s) 935
BsuI GTATCC 1 cut(s) 407
BsuRI GGCC 4 cut(s) 252, 434, 506, 713
BtsCI GGATG 2 cut(s) 361, 626
BtsIMutI CAGTG 1 cut(s) 990
BveI ACCTGC 1 cut(s) 860
Cac8I GCNNGC 1 cut(s) 562
CaiI CAGNNNCTG 1 cut(s) 614
CciI TCATGA 1 cut(s) 319
CfoI GCGC 1 cut(s) 429
Cfr13I GGNCC 5 cut(s) 250, 504, 611, 712, 872
CseI GACGC 1 cut(s) 588
CsiI ACCWGGT 1 cut(s) 613
Csp6I GTAC 3 cut(s) 415, 531, 1072
CviAII CATG 8 cut(s) 242, 291, 320, 424, 555, 565, 661, 745
CviQI GTAC 3 cut(s) 415, 531, 1072
DdeI CTNAG 3 cut(s) 174, 770, 1092
DpnI GATC 5 cut(s) 74, 117, 195, 492, 630
DpnII GATC 5 cut(s) 72, 115, 193, 490, 628
Eam1104I CTCTTC 1 cut(s) 1082
EarI CTCTTC 1 cut(s) 1082
Eco147I AGGCCT 1 cut(s) 434
Eco47I GGWCC 2 cut(s) 611, 872
Eco57I CTGAAG 2 cut(s) 275, 947
EcoO109I RGGNCCY 4 cut(s) 250, 611, 712, 872
EcoRII CCWGG 3 cut(s) 304, 613, 669
FaeI CATG 8 cut(s) 245, 294, 323, 427, 558, 568, 664, 748
FalI AAGNNNNNCTT 2 cut(s) 792, 824
FaqI GGGAC 1 cut(s) 173
FatI CATG 8 cut(s) 241, 290, 319, 423, 554, 564, 660, 744
FbaI TGATCA 1 cut(s) 72
Fnu4HI GCNGC 1 cut(s) 1001
FokI GGATG 2 cut(s) 368, 633
Fsp4HI GCNGC 1 cut(s) 1001
FspBI CTAG 2 cut(s) 119, 1010
GlaI GCGC 1 cut(s) 428
GluI GCNGC 1 cut(s) 1001
GsaI CCCAGC 1 cut(s) 282
HaeIII GGCC 4 cut(s) 252, 434, 506, 713
HapII CCGG 3 cut(s) 105, 507, 632
HgaI GACGC 1 cut(s) 588
HhaI GCGC 1 cut(s) 429
Hin1I GRCGYC 1 cut(s) 599
Hin1II CATG 8 cut(s) 245, 294, 323, 427, 558, 568, 664, 748
Hin6I GCGC 1 cut(s) 427
HinP1I GCGC 1 cut(s) 427
HincII GTYRAC 1 cut(s) 182
HindII GTYRAC 1 cut(s) 182
HindIII AAGCTT 1 cut(s) 257
HinfI GANTC 6 cut(s) 264, 551, 766, 803, 910, 1094
HpaII CCGG 3 cut(s) 105, 507, 632
HphI GGTGA 1 cut(s) 1058
Hpy166II GTNNAC 2 cut(s) 182, 796
Hpy188I TCNGA 3 cut(s) 10, 175, 274
Hpy188III TCNNGA 7 cut(s) 213, 320, 447, 632, 840, 882, 1058
Hpy8I GTNNAC 2 cut(s) 182, 796
Hpy99I CGWCG 1 cut(s) 604
HpyAV CCTTC 3 cut(s) 241, 505, 703
HpyCH4III ACNGT 6 cut(s) 70, 383, 414, 680, 940, 1064
HpyCH4V TGCA 4 cut(s) 92, 335, 608, 752
HpyF10VI GCNNNNNNNGC 3 cut(s) 503, 605, 657
HpyF3I CTNAG 3 cut(s) 174, 770, 1092
Hsp92I GRCGYC 1 cut(s) 599
Hsp92II CATG 8 cut(s) 245, 294, 323, 427, 558, 568, 664, 748
HspAI GCGC 1 cut(s) 427
Kpn2I TCCGGA 1 cut(s) 631
KpnI GGTACC 1 cut(s) 418
Ksp22I TGATCA 1 cut(s) 72
Kzo9I GATC 5 cut(s) 72, 115, 193, 490, 628
Lsp1109I GCAGC 1 cut(s) 1012
LweI GCATC 4 cut(s) 79, 265, 322, 702
MabI ACCWGGT 1 cut(s) 613
MaeI CTAG 2 cut(s) 119, 1010
MaeIII GTNAC 4 cut(s) 383, 665, 1012, 1064
MalI GATC 5 cut(s) 74, 117, 195, 492, 630
MboI GATC 5 cut(s) 72, 115, 193, 490, 628
MboII GAAGA 4 cut(s) 834, 940, 962, 1069
MfeI CAATTG 1 cut(s) 573
MhlI GDGCHC 2 cut(s) 685, 1045
MluCI AATT 5 cut(s) 40, 169, 216, 456, 573
MlyI GAGTC 1 cut(s) 1103
MmeI TCCRAC 1 cut(s) 621
MroI TCCGGA 1 cut(s) 631
MroXI GAANNNNTTC 1 cut(s) 268
MseI TTAA 6 cut(s) 60, 219, 437, 546, 942, 1100
MslI CAYNNNNRTG 1 cut(s) 324
MspA1I CMGCKG 1 cut(s) 86
MspI CCGG 3 cut(s) 105, 507, 632
MspR9I CCNGG 4 cut(s) 306, 508, 615, 671
MunI CAATTG 1 cut(s) 573
MvaI CCWGG 3 cut(s) 306, 615, 671
MvnI CGCG 1 cut(s) 429
MwoI GCNNNNNNNGC 3 cut(s) 503, 605, 657
NciI CCSGG 1 cut(s) 508
NdeII GATC 5 cut(s) 72, 115, 193, 490, 628
NlaIII CATG 8 cut(s) 245, 294, 323, 427, 558, 568, 664, 748
NlaIV GGNNCC 2 cut(s) 416, 714
NmuCI GTSAC 1 cut(s) 1064
NspI RCATGY 1 cut(s) 294
PagI TCATGA 1 cut(s) 319
PceI AGGCCT 1 cut(s) 434
PciI ACATGT 1 cut(s) 290
PdmI GAANNNNTTC 1 cut(s) 268
PfeI GAWTC 5 cut(s) 264, 551, 766, 803, 910
PflFI GACNNNGTC 1 cut(s) 1093
PkrI GCNGC 1 cut(s) 1002
PleI GAGTC 1 cut(s) 1102
PpsI GAGTC 1 cut(s) 1102
PpuMI RGGWCCY 2 cut(s) 611, 872
PscI ACATGT 1 cut(s) 290
PshAI GACNNNNGTC 1 cut(s) 964
PshBI ATTAAT 2 cut(s) 60, 546
Psp5II RGGWCCY 2 cut(s) 611, 872
Psp6I CCWGG 3 cut(s) 304, 613, 669
PspFI CCCAGC 1 cut(s) 278
PspGI CCWGG 3 cut(s) 304, 613, 669
PspN4I GGNNCC 2 cut(s) 416, 714
PspPI GGNCC 5 cut(s) 250, 504, 611, 712, 872
PspPPI RGGWCCY 2 cut(s) 611, 872
PstI CTGCAG 1 cut(s) 754
PstNI CAGNNNCTG 1 cut(s) 614
PsyI GACNNNGTC 1 cut(s) 1093
PvuII CAGCTG 1 cut(s) 86
RsaI GTAC 3 cut(s) 416, 532, 1073
RsaNI GTAC 3 cut(s) 415, 531, 1072
RseI CAYNNNNRTG 1 cut(s) 324
SaqAI TTAA 6 cut(s) 60, 219, 437, 546, 942, 1100
SatI GCNGC 1 cut(s) 1001
Sau3AI GATC 5 cut(s) 72, 115, 193, 490, 628
Sau96I GGNCC 5 cut(s) 250, 504, 611, 712, 872
SchI GAGTC 1 cut(s) 1103
ScrFI CCNGG 4 cut(s) 306, 508, 615, 671
SduI GDGCHC 2 cut(s) 685, 1045
SexAI ACCWGGT 1 cut(s) 613
SfaNI GCATC 4 cut(s) 79, 265, 322, 702
SfcI CTRYAG 1 cut(s) 750
SinI GGWCC 2 cut(s) 611, 872
SmiMI CAYNNNNRTG 1 cut(s) 324
SpeI ACTAGT 1 cut(s) 1009
Sse9I AATT 5 cut(s) 40, 169, 216, 456, 573
SseBI AGGCCT 1 cut(s) 434
SspMI CTAG 2 cut(s) 119, 1010
StuI AGGCCT 1 cut(s) 434
StyD4I CCNGG 4 cut(s) 304, 506, 613, 669
TaaI ACNGT 6 cut(s) 70, 383, 414, 680, 940, 1064
TaqI TCGA 1 cut(s) 301
TasI AATT 5 cut(s) 40, 169, 216, 456, 573
TfiI GAWTC 5 cut(s) 264, 551, 766, 803, 910
Tru1I TTAA 6 cut(s) 60, 219, 437, 546, 942, 1100
Tru9I TTAA 6 cut(s) 60, 219, 437, 546, 942, 1100
TscAI CASTG 1 cut(s) 990
TseFI GTSAC 1 cut(s) 1064
TseI GCWGC 1 cut(s) 1000
Tsp45I GTSAC 1 cut(s) 1064
TspDTI ATGAA 4 cut(s) 78, 336, 543, 722
TspGWI ACGGA 2 cut(s) 466, 1031
TspRI CASTG 1 cut(s) 990
Tth111I GACNNNGTC 1 cut(s) 1093
VpaK11BI GGWCC 2 cut(s) 611, 872
VspI ATTAAT 2 cut(s) 60, 546
XapI RAATTY 2 cut(s) 40, 169
XceI RCATGY 1 cut(s) 294
XcmI CCANNNNNNNNNTGG 1 cut(s) 806
XmnI GAANNNNTTC 1 cut(s) 268
XspI CTAG 2 cut(s) 119, 1010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.