Rh1CG354300

alpha-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0006739
Physical Location & Seq
Reverse (-)
62960146 .. 62964776
4631 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG354300.1

Sequence Viewer

Length: 1080 bp
ATGGCGAAGAGCAAGATCATCACTTTCTCTGTGTTCACTCTGATTCTCTCTCTGTCAGCTCTGGCTATTGCAATTGCAGGCAGAGTAGCTGTGCCTCTGTTGCAATTCCAGGACAAACCCAGTTTCCTGAGACCAACTTTTAGTCGCATTTACGATACTTCAATGTATGGTGCTCTGCAACTCAATAATGGATTGGCTCAAACGCCTCAAATGGGGTGGAATAGCTGGAATTTCTTTGCTTGTGATATTAATGAAACCGTGATCAAGGAAACAGCTGATGCCCTCATCTCAACCGGATTAGCTGATTTAGGTTATGTGTATGTCAATATAGATGATTGCTGGTCTTTACCAACACGAAATTCTGAGGGTCAACTGGTCCCTGATCCTAAAACATTTCCATCGGGAATTAAAGCTCTTGCCGATTATATACATGGGAAGGGCCTTAAGCTTGGAATATATTCTGATGCCGGGGTTTTTACATGTCAAGTTCGACCAGGTTCAATTTATCATGAAAGTGATGATGCCAAGCTGTTTGCTTCTTGGGACTTAGATTACTTGAAGTATGACAACTGTTACAATCTGGGCATCCCACCAAAAGAACGGTACCCACCCATGCGTGAGGCCCTAAATACATCTGGACGCACAATTTTCTATTCAATTTGTGAATGGGGTGTTGATGATCCTGCTTTATGGGCTGGGAAAGTTGGGAATAGTTGGCGTACAACAGATGATATCAATGATACATGGGCAAGCATGACTACAATTGCTGATTTGAATGACAAGTGGGCATCGTATGCAGGACCTGGTGGATGGAATGATCCAGATATGTTGGAAGTTGGCAATGGAGGCATGAGTTACCAGGAATACCGTGCTCATTTTAGCATCTGGGCTTTGATGAAGGCCCCTCTTTTGATTGGTTGTGATGTTAGAAACATGACTGCAGAAACATACGAAATTCTGAGCAATGAGGAGGTTATTGCTATAAACCAAGACTCTCTTGGGGTTCAGGGAAGGAAAGTGAATGTTTCAGGAACAGATGGGGTCTATCAGGTCCGTCCTCCTCAATACATAAAAAAGTGA

Protein Analysis

359

Amino Acids

39.85

Weight (kDa)

4.87

Isoelectric Point (pI)

24.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Melibiase_2 PF16499 68 - 331 4.4e-80 Alpha galactosidase A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 603
AccB1I GGYRCC 1 cut(s) 603
AclWI GGATC 3 cut(s) 377, 674, 812
AcsI RAATTY 3 cut(s) 229, 358, 954
AfaI GTAC 2 cut(s) 605, 721
AfiI CCNNNNNNNGG 1 cut(s) 212
AflII CTTAAG 1 cut(s) 443
AflIII ACRYGT 1 cut(s) 479
AgsI TTSAA 5 cut(s) 162, 501, 559, 657, 775
AjnI CCWGG 4 cut(s) 108, 493, 802, 858
AjuI GAANNNNNNNTTGG 2 cut(s) 343, 375
AluBI AGCT 8 cut(s) 59, 89, 225, 275, 302, 413, 448, 529
AluI AGCT 8 cut(s) 59, 89, 225, 275, 302, 413, 448, 529
Alw21I GWGCWC 2 cut(s) 175, 874
Alw26I GTCTC 1 cut(s) 124
AlwI GGATC 3 cut(s) 377, 674, 812
AlwNI CAGNNNCTG 1 cut(s) 803
AoxI GGCC 3 cut(s) 439, 621, 900
ApoI RAATTY 3 cut(s) 229, 358, 954
AseI ATTAAT 1 cut(s) 249
Asp700I GAANNNNTTC 1 cut(s) 457
Asp718I GGTACC 1 cut(s) 603
AspS9I GGNCC 6 cut(s) 376, 439, 622, 800, 901, 1051
AsuC2I CCSGG 1 cut(s) 469
AvaII GGWCC 3 cut(s) 376, 800, 1051
BanI GGYRCC 1 cut(s) 603
Bbv12I GWGCWC 2 cut(s) 175, 874
BccI CCATC 3 cut(s) 406, 804, 1031
BciT130I CCWGG 4 cut(s) 110, 495, 804, 860
BclI TGATCA 1 cut(s) 261
BcnI CCSGG 1 cut(s) 469
BcoDI GTCTC 1 cut(s) 124
BfmI CTRYAG 1 cut(s) 939
BfrI CTTAAG 1 cut(s) 443
Bme1390I CCNGG 5 cut(s) 110, 469, 495, 804, 860
Bme18I GGWCC 3 cut(s) 376, 800, 1051
BmgT120I GGNCC 6 cut(s) 376, 439, 622, 800, 901, 1051
BmiI GGNNCC 3 cut(s) 378, 605, 903
BmrFI CCNGG 5 cut(s) 110, 469, 495, 804, 860
BmrI ACTGGG 1 cut(s) 114
BmsI GCATC 6 cut(s) 268, 454, 511, 594, 797, 891
BmuI ACTGGG 1 cut(s) 114
BpuMI CCSGG 1 cut(s) 469
BsaI GGTCTC 1 cut(s) 124
BsaJI CCNNGG 1 cut(s) 468
BsaWI WCCGGW 1 cut(s) 293
Bsc4I CCNNNNNNNGG 1 cut(s) 212
Bse1I ACTGG 2 cut(s) 120, 378
Bse3DI GCAATG 2 cut(s) 847, 970
BseBI CCWGG 4 cut(s) 110, 495, 804, 860
BseDI CCNNGG 1 cut(s) 468
BseGI GGATG 2 cut(s) 585, 815
BseLI CCNNNNNNNGG 1 cut(s) 212
BseMI GCAATG 2 cut(s) 847, 970
BseMII CTCAG 3 cut(s) 119, 354, 950
BseNI ACTGG 2 cut(s) 120, 378
BseRI GAGGAG 2 cut(s) 983, 1050
BseYI CCCAGC 1 cut(s) 695
BshFI GGCC 3 cut(s) 441, 623, 902
BshNI GGYRCC 1 cut(s) 603
BsiHKAI GWGCWC 2 cut(s) 175, 874
BsiSI CCGG 2 cut(s) 294, 468
BslFI GGGAC 2 cut(s) 362, 557
BslI CCNNNNNNNGG 1 cut(s) 212
BsmAI GTCTC 1 cut(s) 124
BsmFI GGGAC 2 cut(s) 362, 557
BsnI GGCC 3 cut(s) 441, 623, 902
Bso31I GGTCTC 1 cut(s) 124
Bsp1286I GDGCHC 2 cut(s) 175, 874
Bsp143I GATC 5 cut(s) 15, 261, 382, 679, 817
BspANI GGCC 3 cut(s) 441, 623, 902
BspCNI CTCAG 3 cut(s) 120, 355, 951
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 3 cut(s) 378, 605, 903
BspMAI CTGCAG 1 cut(s) 943
BspPI GGATC 3 cut(s) 377, 674, 812
BspQI GCTCTTC 1 cut(s) 2
BspT107I GGYRCC 1 cut(s) 603
BspTI CTTAAG 1 cut(s) 443
BspTNI GGTCTC 1 cut(s) 124
BsrDI GCAATG 2 cut(s) 847, 970
BsrI ACTGG 2 cut(s) 120, 378
BssECI CCNNGG 1 cut(s) 468
BssMI GATC 5 cut(s) 15, 261, 382, 679, 817
Bst2UI CCWGG 4 cut(s) 110, 495, 804, 860
Bst4CI ACNGT 4 cut(s) 259, 572, 603, 869
Bst6I CTCTTC 1 cut(s) 2
BstAFI CTTAAG 1 cut(s) 443
BstAPI GCANNNNNTGC 1 cut(s) 794
BstC8I GCNNGC 2 cut(s) 79, 751
BstDEI CTNAG 4 cut(s) 128, 363, 547, 959
BstF5I GGATG 2 cut(s) 585, 815
BstKTI GATC 5 cut(s) 18, 264, 385, 682, 820
BstMAI GTCTC 1 cut(s) 124
BstMBI GATC 5 cut(s) 15, 261, 382, 679, 817
BstMWI GCNNNNNNNGC 4 cut(s) 100, 692, 794, 846
BstNI CCWGG 4 cut(s) 110, 495, 804, 860
BstNSI RCATGY 1 cut(s) 483
BstSCI CCNGG 5 cut(s) 108, 467, 493, 802, 858
BstSFI CTRYAG 1 cut(s) 939
BsuRI GGCC 3 cut(s) 441, 623, 902
BtsCI GGATG 2 cut(s) 585, 815
Cac8I GCNNGC 2 cut(s) 79, 751
CaiI CAGNNNCTG 1 cut(s) 803
CciI TCATGA 1 cut(s) 508
Cfr13I GGNCC 6 cut(s) 376, 439, 622, 800, 901, 1051
CseI GACGC 1 cut(s) 648
CsiI ACCWGGT 2 cut(s) 493, 802
Csp6I GTAC 2 cut(s) 604, 720
CspCI CAANNNNNGTGG 2 cut(s) 197, 232
CviAII CATG 8 cut(s) 431, 480, 509, 613, 744, 754, 850, 934
CviQI GTAC 2 cut(s) 604, 720
DdeI CTNAG 4 cut(s) 128, 363, 547, 959
DpnI GATC 5 cut(s) 17, 263, 384, 681, 819
DpnII GATC 5 cut(s) 15, 261, 382, 679, 817
Eam1104I CTCTTC 1 cut(s) 2
EarI CTCTTC 1 cut(s) 2
Eco31I GGTCTC 1 cut(s) 124
Eco32I GATATC 1 cut(s) 733
Eco47I GGWCC 3 cut(s) 376, 800, 1051
EcoO109I RGGNCCY 4 cut(s) 439, 622, 800, 901
EcoRII CCWGG 4 cut(s) 108, 493, 802, 858
EcoRV GATATC 1 cut(s) 733
FaeI CATG 8 cut(s) 434, 483, 512, 616, 747, 757, 853, 937
FalI AAGNNNNNCTT 2 cut(s) 981, 1013
FaqI GGGAC 2 cut(s) 362, 557
FatI CATG 8 cut(s) 430, 479, 508, 612, 743, 753, 849, 933
FbaI TGATCA 1 cut(s) 261
FokI GGATG 2 cut(s) 572, 822
GsaI CCCAGC 1 cut(s) 699
HaeIII GGCC 3 cut(s) 441, 623, 902
HapII CCGG 2 cut(s) 294, 468
HgaI GACGC 1 cut(s) 648
Hin1II CATG 8 cut(s) 434, 483, 512, 616, 747, 757, 853, 937
HincII GTYRAC 1 cut(s) 371
HindII GTYRAC 1 cut(s) 371
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 2 cut(s) 43, 992
HpaII CCGG 2 cut(s) 294, 468
Hpy166II GTNNAC 2 cut(s) 36, 371
Hpy188I TCNGA 4 cut(s) 42, 364, 463, 960
Hpy188III TCNNGA 6 cut(s) 127, 402, 509, 636, 821, 1029
Hpy8I GTNNAC 2 cut(s) 36, 371
HpyAV CCTTC 3 cut(s) 430, 892, 1005
HpyCH4III ACNGT 4 cut(s) 259, 572, 603, 869
HpyCH4V TGCA 6 cut(s) 71, 77, 103, 178, 797, 941
HpyF10VI GCNNNNNNNGC 4 cut(s) 100, 692, 794, 846
HpyF3I CTNAG 4 cut(s) 128, 363, 547, 959
Hsp92II CATG 8 cut(s) 434, 483, 512, 616, 747, 757, 853, 937
KpnI GGTACC 1 cut(s) 607
Ksp22I TGATCA 1 cut(s) 261
Kzo9I GATC 5 cut(s) 15, 261, 382, 679, 817
LguI GCTCTTC 1 cut(s) 2
LweI GCATC 6 cut(s) 268, 454, 511, 594, 797, 891
MabI ACCWGGT 2 cut(s) 493, 802
MaeIII GTNAC 2 cut(s) 572, 854
MalI GATC 5 cut(s) 17, 263, 384, 681, 819
MboI GATC 5 cut(s) 15, 261, 382, 679, 817
MboII GAAGA 1 cut(s) 19
MfeI CAATTG 2 cut(s) 72, 762
MhlI GDGCHC 2 cut(s) 175, 874
MlyI GAGTC 1 cut(s) 986
MmeI TCCRAC 1 cut(s) 810
MroXI GAANNNNTTC 1 cut(s) 457
MseI TTAA 3 cut(s) 249, 408, 444
MslI CAYNNNNRTG 1 cut(s) 513
MspA1I CMGCKG 1 cut(s) 275
MspCI CTTAAG 1 cut(s) 443
MspI CCGG 2 cut(s) 294, 468
MspR9I CCNGG 5 cut(s) 110, 469, 495, 804, 860
MunI CAATTG 2 cut(s) 72, 762
MvaI CCWGG 4 cut(s) 110, 495, 804, 860
MwoI GCNNNNNNNGC 4 cut(s) 100, 692, 794, 846
NciI CCSGG 1 cut(s) 469
NdeII GATC 5 cut(s) 15, 261, 382, 679, 817
NlaIII CATG 8 cut(s) 434, 483, 512, 616, 747, 757, 853, 937
NlaIV GGNNCC 3 cut(s) 378, 605, 903
NspI RCATGY 1 cut(s) 483
PagI TCATGA 1 cut(s) 508
PciI ACATGT 1 cut(s) 479
PciSI GCTCTTC 1 cut(s) 2
PdmI GAANNNNTTC 1 cut(s) 457
PfeI GAWTC 1 cut(s) 43
PfoI TCCNGGA 1 cut(s) 108
PleI GAGTC 1 cut(s) 986
PpsI GAGTC 1 cut(s) 986
PpuMI RGGWCCY 1 cut(s) 800
PscI ACATGT 1 cut(s) 479
PshBI ATTAAT 1 cut(s) 249
Psp5II RGGWCCY 1 cut(s) 800
Psp6I CCWGG 4 cut(s) 108, 493, 802, 858
PspFI CCCAGC 1 cut(s) 695
PspGI CCWGG 4 cut(s) 108, 493, 802, 858
PspN4I GGNNCC 3 cut(s) 378, 605, 903
PspPI GGNCC 6 cut(s) 376, 439, 622, 800, 901, 1051
PspPPI RGGWCCY 1 cut(s) 800
PstI CTGCAG 1 cut(s) 943
PstNI CAGNNNCTG 1 cut(s) 803
PvuII CAGCTG 1 cut(s) 275
RsaI GTAC 2 cut(s) 605, 721
RsaNI GTAC 2 cut(s) 604, 720
RseI CAYNNNNRTG 1 cut(s) 513
SapI GCTCTTC 1 cut(s) 2
SaqAI TTAA 3 cut(s) 249, 408, 444
Sau3AI GATC 5 cut(s) 15, 261, 382, 679, 817
Sau96I GGNCC 6 cut(s) 376, 439, 622, 800, 901, 1051
SchI GAGTC 1 cut(s) 986
ScrFI CCNGG 5 cut(s) 110, 469, 495, 804, 860
SduI GDGCHC 2 cut(s) 175, 874
SexAI ACCWGGT 2 cut(s) 493, 802
SfaNI GCATC 6 cut(s) 268, 454, 511, 594, 797, 891
SfcI CTRYAG 1 cut(s) 939
SinI GGWCC 3 cut(s) 376, 800, 1051
SmiMI CAYNNNNRTG 1 cut(s) 513
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
StyD4I CCNGG 5 cut(s) 108, 467, 493, 802, 858
TaaI ACNGT 4 cut(s) 259, 572, 603, 869
TaqI TCGA 1 cut(s) 490
TfiI GAWTC 1 cut(s) 43
Tru1I TTAA 3 cut(s) 249, 408, 444
Tru9I TTAA 3 cut(s) 249, 408, 444
TspDTI ATGAA 3 cut(s) 267, 525, 911
TspGWI ACGGA 1 cut(s) 1043
Vha464I CTTAAG 1 cut(s) 443
VpaK11BI GGWCC 3 cut(s) 376, 800, 1051
VspI ATTAAT 1 cut(s) 249
XapI RAATTY 3 cut(s) 229, 358, 954
XceI RCATGY 1 cut(s) 483
XcmI CCANNNNNNNNNTGG 1 cut(s) 995
XmnI GAANNNNTTC 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.