Rorug01G0368900

alpha-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0006739
Physical Location & Seq
Reverse (-)
48368470 .. 48370269
1800 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0368900.1

Sequence Viewer

Length: 1026 bp
ATGAAAAATAGAGGTGGGTGCGGCTGTGTGCTGCGGCACTGTTCGGAAGAGAGAGAATTGAGGGATTGTCAAGAGTCTGCTATTGAGCAGCCTTCTGCAAATCATGTTGAGAATAATGTTGAGGAATCTGCTGGTGTTGGTCAGAACGTGGTAGTGCAGTGTGAGGTGGAGCACACAAACCGGTTCCAGCAATTAGCTGCTGATGGGTTGGATGGAAATAGTAGTGGTGAAGATAATAGTGATGATTCTGCTGAGAGTGAAGAGGAGTTAGAGCAAGTGGCTGCTGCCCATGCTACTTCCTCCAACACAATTGTGGATTGGTATGAAGAGACAAAAAAAACTGCAGAGGCAGATGGTTTCACCGAGATATTGTATTGGAATTTACGAGGCATTGGTAATGTCTCTACTCAAGATGCATTGTCAGAATTTGTTCGTGTTAATAATCCTGAGCTATTGTGCATAGCTGAACCCTTTGTTTCATTGGATGCTATTCCATCTTGCTTTTGGCGTTCCATGGGTATGGTGGCGGTGTGTACTAATGACAGGGCTGCTTTATTGCCTAATTTATGGGTTTTTTGTAAACTTTCTCTTGTACCGTTTGTTCGTGTCCTCTCTATCACAGACCAACAAGTTTCTCTTCAAGTTATGTTTGATTCGGTGAATTGTATTATTACGGCTGTATATGCTTGCACAACAACTGCTAGTCGTCGTCAATTATGGGAAGATATCACTAATGTTAAAGGCAGTTTTGTTTCTGGTCCATGGCTTGTATTTGGTGATTTTAATGCAATTTTAGGGGCTCATGAGAAGAAGGGGGGTGGTTCTGTTTATGCGAGCTCTTGTGCAGAATTTCAGGCAATGTCTGACGTTTGTGAGCTTGTGCATGTGGATACCAAAGGAGCAGAGTTTACCTGGGTTCGTCGGAGGGATCTCCGAGGTAATGTAGAAATAAGGTTGGATCGTAGTCTTGCAAATCTTTCTTGGTTGGATGTTTGGGATCAGTTTGATTGTTGTACATTGCCTTGA

Protein Analysis

341

Amino Acids

37.73

Weight (kDa)

4.51

Isoelectric Point (pI)

47.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 124 - 328 3.9e-06 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 21, 34, 527
AclWI GGATC 3 cut(s) 936, 966, 1005
AcsI RAATTY 3 cut(s) 379, 425, 848
AfaI GTAC 3 cut(s) 535, 594, 1015
AgeI ACCGGT 1 cut(s) 180
AgsI TTSAA 1 cut(s) 641
AjnI CCWGG 1 cut(s) 911
AleI CACNNNNGTG 1 cut(s) 311
AluBI AGCT 5 cut(s) 197, 451, 464, 837, 877
AluI AGCT 5 cut(s) 197, 451, 464, 837, 877
Alw21I GWGCWC 2 cut(s) 174, 839
Alw26I GTCTC 2 cut(s) 323, 406
AlwI GGATC 3 cut(s) 936, 966, 1005
ApeKI GCWGC 6 cut(s) 31, 88, 197, 281, 284, 548
ApoI RAATTY 3 cut(s) 379, 425, 848
AsiGI ACCGGT 1 cut(s) 180
Asp700I GAANNNNTTC 1 cut(s) 429
AspS9I GGNCC 1 cut(s) 758
AsuHPI GGTGA 4 cut(s) 239, 352, 670, 788
AvaII GGWCC 1 cut(s) 758
BanII GRGCYC 2 cut(s) 802, 839
Bbv12I GWGCWC 2 cut(s) 174, 839
BbvI GCAGC 6 cut(s) 18, 100, 184, 268, 271, 535
BccI CCATC 4 cut(s) 197, 206, 347, 502
BceAI ACGGC 1 cut(s) 690
BciT130I CCWGG 1 cut(s) 913
BciVI GTATCC 1 cut(s) 883
BcoDI GTCTC 2 cut(s) 323, 406
BfaI CTAG 1 cut(s) 702
BfmI CTRYAG 1 cut(s) 342
BfuI GTATCC 1 cut(s) 883
BisI GCNGC 8 cut(s) 22, 32, 35, 89, 198, 282, 285, 549
BlsI GCNGC 8 cut(s) 23, 33, 36, 90, 199, 283, 286, 550
Bme1390I CCNGG 1 cut(s) 913
Bme18I GGWCC 1 cut(s) 758
BmgT120I GGNCC 1 cut(s) 758
BmiI GGNNCC 1 cut(s) 185
BmrFI CCNGG 1 cut(s) 913
BmsI GCATC 2 cut(s) 403, 475
Bpu10I CCTNAGC 1 cut(s) 447
BpuEI CTTGAG 1 cut(s) 393
BsaJI CCNNGG 4 cut(s) 513, 761, 912, 934
BsaWI WCCGGW 1 cut(s) 180
Bse118I RCCGGY 1 cut(s) 180
Bse3DI GCAATG 2 cut(s) 864, 1016
BseBI CCWGG 1 cut(s) 913
BseDI CCNNGG 4 cut(s) 513, 761, 912, 934
BseGI GGATG 3 cut(s) 217, 490, 994
BseMI GCAATG 2 cut(s) 864, 1016
BseMII CTCAG 2 cut(s) 243, 438
BseRI GAGGAG 1 cut(s) 278
BseXI GCAGC 6 cut(s) 18, 100, 184, 268, 271, 535
BsgI GTGCAG 2 cut(s) 176, 864
BshTI ACCGGT 1 cut(s) 180
BsiHKAI GWGCWC 2 cut(s) 174, 839
BsiSI CCGG 1 cut(s) 181
BsmAI GTCTC 2 cut(s) 323, 406
Bsp1286I GDGCHC 3 cut(s) 174, 802, 839
Bsp1407I TGTACA 1 cut(s) 1013
Bsp143I GATC 3 cut(s) 928, 958, 997
Bsp19I CCATGG 2 cut(s) 513, 761
BspACI CCGC 3 cut(s) 21, 34, 527
BspCNI CTCAG 2 cut(s) 244, 439
BspHI TCATGA 1 cut(s) 802
BspLI GGNNCC 1 cut(s) 185
BspMAI CTGCAG 1 cut(s) 346
BspPI GGATC 3 cut(s) 936, 966, 1005
BsrDI GCAATG 2 cut(s) 864, 1016
BsrFI RCCGGY 1 cut(s) 180
BsrGI TGTACA 1 cut(s) 1013
BssAI RCCGGY 1 cut(s) 180
BssECI CCNNGG 4 cut(s) 513, 761, 912, 934
BssMI GATC 3 cut(s) 928, 958, 997
BssT1I CCWWGG 2 cut(s) 513, 761
Bst2UI CCWGG 1 cut(s) 913
Bst4CI ACNGT 2 cut(s) 41, 597
Bst6I CTCTTC 4 cut(s) 42, 255, 321, 642
BstAUI TGTACA 1 cut(s) 1013
BstC8I GCNNGC 2 cut(s) 688, 835
BstDEI CTNAG 2 cut(s) 252, 447
BstDSI CCRYGG 2 cut(s) 513, 761
BstF5I GGATG 3 cut(s) 217, 490, 994
BstKTI GATC 3 cut(s) 931, 961, 1000
BstMAI GTCTC 2 cut(s) 323, 406
BstMBI GATC 3 cut(s) 928, 958, 997
BstMWI GCNNNNNNNGC 2 cut(s) 290, 683
BstNI CCWGG 1 cut(s) 913
BstNSI RCATGY 1 cut(s) 887
BstSCI CCNGG 1 cut(s) 911
BstSFI CTRYAG 1 cut(s) 342
BstV1I GCAGC 6 cut(s) 18, 100, 184, 268, 271, 535
BstX2I RGATCY 1 cut(s) 928
BstXI CCANNNNNNTGG 1 cut(s) 520
BstYI RGATCY 1 cut(s) 928
BsuI GTATCC 1 cut(s) 883
BtgI CCRYGG 2 cut(s) 513, 761
BtsCI GGATG 3 cut(s) 217, 490, 994
BtsI GCAGTG 1 cut(s) 164
BtsIMutI CAGTG 2 cut(s) 37, 164
Cac8I GCNNGC 2 cut(s) 688, 835
CciI TCATGA 1 cut(s) 802
Cfr10I RCCGGY 1 cut(s) 180
Cfr13I GGNCC 1 cut(s) 758
Csp6I GTAC 3 cut(s) 534, 593, 1014
CspAI ACCGGT 1 cut(s) 180
CviAII CATG 6 cut(s) 104, 290, 514, 762, 803, 884
CviQI GTAC 3 cut(s) 534, 593, 1014
DdeI CTNAG 2 cut(s) 252, 447
DpnI GATC 3 cut(s) 930, 960, 999
DpnII GATC 3 cut(s) 928, 958, 997
Eam1104I CTCTTC 4 cut(s) 42, 255, 321, 642
EarI CTCTTC 4 cut(s) 42, 255, 321, 642
Ecl136II GAGCTC 1 cut(s) 837
Eco130I CCWWGG 2 cut(s) 513, 761
Eco24I GRGCYC 2 cut(s) 802, 839
Eco32I GATATC 1 cut(s) 727
Eco47I GGWCC 1 cut(s) 758
Eco53kI GAGCTC 1 cut(s) 837
EcoICRI GAGCTC 1 cut(s) 837
EcoRII CCWGG 1 cut(s) 911
EcoRV GATATC 1 cut(s) 727
EcoT14I CCWWGG 2 cut(s) 513, 761
EcoT22I ATGCAT 1 cut(s) 418
EcoT38I GRGCYC 2 cut(s) 802, 839
ErhI CCWWGG 2 cut(s) 513, 761
FaeI CATG 6 cut(s) 107, 293, 517, 765, 806, 887
FalI AAGNNNNNCTT 2 cut(s) 621, 653
FatI CATG 6 cut(s) 103, 289, 513, 761, 802, 883
Fnu4HI GCNGC 8 cut(s) 22, 32, 35, 89, 198, 282, 285, 549
FokI GGATG 3 cut(s) 224, 497, 1001
FriOI GRGCYC 2 cut(s) 802, 839
Fsp4HI GCNGC 8 cut(s) 22, 32, 35, 89, 198, 282, 285, 549
FspBI CTAG 1 cut(s) 702
GluI GCNGC 8 cut(s) 22, 32, 35, 89, 198, 282, 285, 549
HapII CCGG 1 cut(s) 181
Hin1II CATG 6 cut(s) 107, 293, 517, 765, 806, 887
HinfI GANTC 4 cut(s) 74, 125, 245, 653
HpaII CCGG 1 cut(s) 181
HphI GGTGA 4 cut(s) 239, 352, 670, 788
Hpy166II GTNNAC 3 cut(s) 534, 581, 909
Hpy188I TCNGA 6 cut(s) 46, 144, 424, 865, 924, 935
Hpy188III TCNNGA 4 cut(s) 71, 410, 446, 803
Hpy8I GTNNAC 3 cut(s) 534, 581, 909
Hpy99I CGWCG 2 cut(s) 711, 924
HpyAV CCTTC 2 cut(s) 102, 805
HpyCH4III ACNGT 2 cut(s) 41, 597
HpyCH4IV ACGT 2 cut(s) 147, 867
HpyF10VI GCNNNNNNNGC 2 cut(s) 290, 683
HpyF3I CTNAG 2 cut(s) 252, 447
HpySE526I ACGT 2 cut(s) 147, 867
Hsp92II CATG 6 cut(s) 107, 293, 517, 765, 806, 887
Kzo9I GATC 3 cut(s) 928, 958, 997
LmnI GCTCC 2 cut(s) 169, 899
LpnPI CCDG 9 cut(s) 117, 194, 200, 459, 529, 741, 839, 898, 925
Lsp1109I GCAGC 6 cut(s) 18, 100, 184, 268, 271, 535
LweI GCATC 2 cut(s) 403, 475
MaeI CTAG 1 cut(s) 702
MaeII ACGT 2 cut(s) 147, 867
MalI GATC 3 cut(s) 930, 960, 999
MboI GATC 3 cut(s) 928, 958, 997
MboII GAAGA 7 cut(s) 59, 242, 272, 338, 629, 734, 820
MfeI CAATTG 1 cut(s) 309
MflI RGATCY 1 cut(s) 928
MhlI GDGCHC 3 cut(s) 174, 802, 839
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 5 cut(s) 189, 327, 902, 936, 966
Mph1103I ATGCAT 1 cut(s) 418
MroXI GAANNNNTTC 1 cut(s) 429
MseI TTAA 3 cut(s) 438, 738, 783
MslI CAYNNNNRTG 2 cut(s) 311, 518
MspI CCGG 1 cut(s) 181
MspR9I CCNGG 1 cut(s) 913
MunI CAATTG 1 cut(s) 309
MvaI CCWGG 1 cut(s) 913
MwoI GCNNNNNNNGC 2 cut(s) 290, 683
NcoI CCATGG 2 cut(s) 513, 761
NdeII GATC 3 cut(s) 928, 958, 997
NlaIII CATG 6 cut(s) 107, 293, 517, 765, 806, 887
NlaIV GGNNCC 1 cut(s) 185
NsiI ATGCAT 1 cut(s) 418
NspI RCATGY 1 cut(s) 887
OliI CACNNNNGTG 1 cut(s) 311
PagI TCATGA 1 cut(s) 802
PdmI GAANNNNTTC 1 cut(s) 429
PfeI GAWTC 3 cut(s) 125, 245, 653
PinAI ACCGGT 1 cut(s) 180
PkrI GCNGC 8 cut(s) 23, 33, 36, 90, 199, 283, 286, 550
PleI GAGTC 1 cut(s) 82
PpsI GAGTC 1 cut(s) 82
Psp124BI GAGCTC 1 cut(s) 839
Psp6I CCWGG 1 cut(s) 911
PspGI CCWGG 1 cut(s) 911
PspN4I GGNNCC 1 cut(s) 185
PspPI GGNCC 1 cut(s) 758
PsrI GAACNNNNNNTAC 2 cut(s) 585, 617
PstI CTGCAG 1 cut(s) 346
PsuI RGATCY 1 cut(s) 928
RsaI GTAC 3 cut(s) 535, 594, 1015
RsaNI GTAC 3 cut(s) 534, 593, 1014
RseI CAYNNNNRTG 2 cut(s) 311, 518
SacI GAGCTC 1 cut(s) 839
SaqAI TTAA 3 cut(s) 438, 738, 783
SatI GCNGC 8 cut(s) 22, 32, 35, 89, 198, 282, 285, 549
Sau3AI GATC 3 cut(s) 928, 958, 997
Sau96I GGNCC 1 cut(s) 758
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 1 cut(s) 913
SduI GDGCHC 3 cut(s) 174, 802, 839
SfaNI GCATC 2 cut(s) 403, 475
SfcI CTRYAG 1 cut(s) 342
SinI GGWCC 1 cut(s) 758
SmiMI CAYNNNNRTG 2 cut(s) 311, 518
SmlI CTYRAG 1 cut(s) 408
SmoI CTYRAG 1 cut(s) 408
SsiI CCGC 3 cut(s) 21, 34, 527
SspMI CTAG 1 cut(s) 702
SstI GAGCTC 1 cut(s) 839
StyD4I CCNGG 1 cut(s) 911
StyI CCWWGG 2 cut(s) 513, 761
TaaI ACNGT 2 cut(s) 41, 597
TaiI ACGT 2 cut(s) 150, 870
TatI WGTACW 2 cut(s) 533, 1013
TauI GCSGC 2 cut(s) 24, 37
TfiI GAWTC 3 cut(s) 125, 245, 653
Tru1I TTAA 3 cut(s) 438, 738, 783
Tru9I TTAA 3 cut(s) 438, 738, 783
TscAI CASTG 2 cut(s) 44, 164
TseI GCWGC 6 cut(s) 31, 88, 197, 281, 284, 548
TspDTI ATGAA 3 cut(s) 17, 339, 468
TspRI CASTG 2 cut(s) 44, 164
VpaK11BI GGWCC 1 cut(s) 758
XapI RAATTY 3 cut(s) 379, 425, 848
XceI RCATGY 1 cut(s) 887
XcmI CCANNNNNNNNNTGG 3 cut(s) 310, 501, 520
XmnI GAANNNNTTC 1 cut(s) 429
XspI CTAG 1 cut(s) 702
Zsp2I ATGCAT 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.